id: A0A494B8X4
gene_symbol: Sdhaf2
taxon:
  id: NCBITaxon:10090
  label: Mus musculus
status: COMPLETE
description: Mitochondrial targeting is supported by a retained transit peptide. The shorter Sdhaf2 product’s
  ability to promote SDHA flavinylation and respiratory-complex maturation remains uncertain.
source_documents:
- genes/mouse/Sdhaf2/Sdhaf2-protnlm-source.json
- genes/mouse/Sdhaf2/Sdhaf2-notes.md
references:
- id: GO_REF:0000044
  title: Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping,
    accompanied by conservative changes to GO terms applied by UniProt
  findings: []
- id: GO_REF:0000117
  title: Electronic Gene Ontology annotations created by ARBA machine learning models
  findings: []
- id: PMID:38569044
  title: Drp1 controls complex II assembly and skeletal muscle metabolism by Sdhaf2 action on mitochondria.
  findings:
  - statement: Mouse muscle and myocyte experiments connect mitochondrial Sdhaf2 availability to complex-II
      assembly and activity; they do not isolate the 135-residue alternative product.
    supporting_text: 'Restoration of Sdhaf2 normalized complex II

      activity, lipid oxidation, and insulin action in Drp1-KD myocytes.'
  full_text_unavailable: false
  reference_review:
    relevance: HIGH
    correctness: VERIFIED
    review_notes: Primary report checked against the cached abstract/full text; the evidence scope is
      stated in the finding.
- id: PMID:23983127
  title: Succinate dehydrogenase 5 (SDH5) regulates glycogen synthase kinase 3β-β-catenin-mediated lung
    cancer metastasis.
  findings:
  - statement: The study directly reports a mouse Sdhaf2/SDH5 loss phenotype in lung epithelial cells,
      so the EMT association is not supported solely by an ARBA assertion.
    supporting_text: In SDH5 knock-out mice, lung epithelial cells exhibited elevated mesenchymal markers,
      which is characteristic of EMT.
  - statement: Human lung-cell experiments link SDH5 to GSK3-beta/beta-catenin regulation in a context-dependent
      mechanism, rather than establishing a universal Wnt effect of all products.
    supporting_text: SDH5 functions as a negative regulator of Wnt-β-catenin signaling.
  full_text_unavailable: false
  reference_review:
    relevance: HIGH
    correctness: VERIFIED
    review_notes: Primary report checked against the cached abstract/full text; the evidence scope is
      stated in the finding.
- id: file:mouse/Sdhaf2/Sdhaf2-bioinformatics/RESULTS.md
  title: Exact Sdhaf2 product and mitochondrial-targeting sequence comparison
- id: file:mouse/Sdhaf2/Sdhaf2-protnlm-source.json
  title: Frozen exact-input ProtNLM output and UniProt sequence for A0A494B8X4
predictions:
- source_method: ProtNLM2
  source_version: UniProt API snapshot 2026-09-10
  source_reference_id: file:mouse/Sdhaf2/Sdhaf2-protnlm-source.json
  predicted_term:
    id: GO:0018293
    label: protein-FAD linkage
  predicted_term_type: GO_BP
  review:
    assessment: UNC
    confidence_score: 1
    summary: Protein-FAD linkage is the established maturation role of SDHAF2, but the selected product’s
      altered C-terminal region has not been functionally tested. Preservation of the targeting peptide
      establishes a plausible compartment, not complete SDHA binding or flavinylation.
    supported_by:
    - &id001
      reference_id: PMID:38569044
      supporting_text: 'Restoration of Sdhaf2 normalized complex II

        activity, lipid oxidation, and insulin action in Drp1-KD myocytes.'
    - &id002
      reference_id: file:mouse/Sdhaf2/Sdhaf2-bioinformatics/RESULTS.md
      supporting_text: '| Transit peptide: Mitochondrion | 1–27 | 27 / 27 | 27 |'
- source_method: ProtNLM2
  source_version: UniProt API snapshot 2026-09-10
  source_reference_id: file:mouse/Sdhaf2/Sdhaf2-protnlm-source.json
  predicted_term:
    id: GO:0005759
    label: mitochondrial matrix
  predicted_term_type: GO_CC
  review:
    assessment: CNN
    confidence_score: 2
    summary: The matrix compartment agrees with the gene-level mitochondrial mechanism and is independently
      supported by complete retention of the reference transit peptide. It overlaps the existing GOA compartment,
      without establishing literal training-set membership.
    supported_by:
    - *id001
    - *id002
- source_method: ProtNLM2
  source_version: UniProt API snapshot 2026-09-10
  source_reference_id: file:mouse/Sdhaf2/Sdhaf2-protnlm-source.json
  predicted_term:
    id: GO:0006121
    label: mitochondrial electron transport, succinate to ubiquinone
  predicted_term_type: GO_BP
  review:
    assessment: UNC
    confidence_score: 1
    summary: Sdhaf2 enables assembly of the complex that carries out succinate-to-ubiquinone electron
      transport, but is not itself an electron-transfer subunit. A contribution to the process through
      assembly is plausible; for this altered product, preservation of that assembly function is unresolved.
    supported_by:
    - *id001
    - *id002