ProtNLM2 External predictions
View prediction review YAML · Spcs2-protnlm-predictions-review.yaml · Review status: COMPLETE
Peptidase activity is incorrectly transferred to a short derivative of the noncatalytic SPCS2 subunit. Signal-peptide processing and complex membership remain uncertain for a product lacking the reference membrane-spanning segments.
Source documents: genes/mouse/Spcs2/Spcs2-protnlm-source.json · genes/mouse/Spcs2/Spcs2-notes.md
Review score: 2 = concordant with evidence; 1 = uncertain; 0 = discordant with evidence. This is an assessment score, not a model probability.
NPI — Nonparalog incorrect Review score: 0/2
Prediction method: ProtNLM2 · Version: UniProt API snapshot 2026-09-10 · file:mouse/Spcs2/Spcs2-protnlm-source.json
COMPLEX ACTIVITY TRANSFER
Review rationale: SPCS2 is an accessory subunit; SEC11A/C contain the signal-peptidase catalytic site. The selected product additionally lacks the reference transmembrane architecture. Catalysis by the complex does not establish peptidase activity of this protein.
Supporting Evidence:
- PMID:42173868: "the catalytic SPC subunit (paralog Sec11A or Sec11C, respectively) is joined by SPCS1 (also known as SPC12), SPCS2 (SPC25), and SPCS3 (SPC22/23)."
- file:mouse/Spcs2/Spcs2-bioinformatics/RESULTS.md: "Selected A0A140LHW5 has 74 residues; reference Q9CYN2 has 226 residues."
UNC — Uncertain Review score: 1/2
Prediction method: ProtNLM2 · Version: UniProt API snapshot 2026-09-10 · file:mouse/Spcs2/Spcs2-protnlm-source.json
Review rationale: Full-length SPCS2 contributes to signal peptide processing as a component of the signal peptidase complex. A0A140LHW5 lacks both reference transmembrane helices and has no demonstrated complex incorporation or processing phenotype; the process cannot be transferred confidently to this exact product.
Supporting Evidence:
- PMID:42173868: "the catalytic SPC subunit (paralog Sec11A or Sec11C, respectively) is joined by SPCS1 (also known as SPC12), SPCS2 (SPC25), and SPCS3 (SPC22/23)."
- file:mouse/Spcs2/Spcs2-bioinformatics/RESULTS.md: "Selected A0A140LHW5 has 74 residues; reference Q9CYN2 has 226 residues."
UNC — Uncertain Review score: 1/2
Prediction method: ProtNLM2 · Version: UniProt API snapshot 2026-09-10 · file:mouse/Spcs2/Spcs2-protnlm-source.json
Review rationale: Same-gene identity supports an SPCS2 origin, but the 74-residue selected sequence retains only the N-terminal segment of the 226-residue membrane subunit. Membership in an assembled signal peptidase complex requires evidence that this short product is expressed and incorporated.
Supporting Evidence:
- file:mouse/Spcs2/Spcs2-bioinformatics/RESULTS.md: "Selected A0A140LHW5 has 74 residues; reference Q9CYN2 has 226 residues."
- PMID:42173868: "the catalytic SPC subunit (paralog Sec11A or Sec11C, respectively) is joined by SPCS1 (also known as SPC12), SPCS2 (SPC25), and SPCS3 (SPC22/23)."