id: A0A140LHW5
gene_symbol: Spcs2
taxon:
  id: NCBITaxon:10090
  label: Mus musculus
status: COMPLETE
description: Peptidase activity is incorrectly transferred to a short derivative of the noncatalytic SPCS2
  subunit. Signal-peptide processing and complex membership remain uncertain for a product lacking the
  reference membrane-spanning segments.
source_documents:
- genes/mouse/Spcs2/Spcs2-protnlm-source.json
- genes/mouse/Spcs2/Spcs2-notes.md
references:
- id: PMID:34388369
  title: Structure of the human signal peptidase complex reveals the determinants for signal peptide cleavage.
  findings:
  - statement: Human structural analysis identifies distinct catalytic SEC11 subunits within the four-subunit
      signal peptidase complex.
    supporting_text: "the \nhuman SPC exists in two functional paralogs with distinct proteolytic subunits."
  full_text_unavailable: true
  reference_review:
    relevance: HIGH
    correctness: VERIFIED
    review_notes: Primary report checked against the cached abstract/full text; the evidence scope is
      stated in the finding.
- id: PMID:42173868
  title: Structural basis of signal peptide recognition by the signal peptidase complex.
  findings:
  - statement: The substrate-bound human structure distinguishes SPCS2 from the catalytic SEC11 subunit.
    supporting_text: the catalytic SPC subunit (paralog Sec11A or Sec11C, respectively) is joined by SPCS1
      (also known as SPC12), SPCS2 (SPC25), and SPCS3 (SPC22/23).
  full_text_unavailable: false
  reference_review:
    relevance: HIGH
    correctness: VERIFIED
    review_notes: Primary report checked against the cached abstract/full text; the evidence scope is
      stated in the finding.
- id: file:mouse/Spcs2/Spcs2-bioinformatics/RESULTS.md
  title: Exact Spcs2 product-to-reference sequence mapping
- id: file:mouse/Spcs2/Spcs2-protnlm-source.json
  title: Frozen exact-input ProtNLM output and UniProt sequence for A0A140LHW5
predictions:
- source_method: ProtNLM2
  source_version: UniProt API snapshot 2026-09-10
  source_reference_id: file:mouse/Spcs2/Spcs2-protnlm-source.json
  predicted_term:
    id: GO:0008233
    label: peptidase activity
  predicted_term_type: GO_MF
  review:
    assessment: NPI
    error_type: COMPLEX_ACTIVITY_TRANSFER
    confidence_score: 0
    summary: SPCS2 is an accessory subunit; SEC11A/C contain the signal-peptidase catalytic site. The
      selected product additionally lacks the reference transmembrane architecture. Catalysis by the complex
      does not establish peptidase activity of this protein.
    supported_by:
    - &id001
      reference_id: PMID:42173868
      supporting_text: the catalytic SPC subunit (paralog Sec11A or Sec11C, respectively) is joined by
        SPCS1 (also known as SPC12), SPCS2 (SPC25), and SPCS3 (SPC22/23).
    - &id002
      reference_id: file:mouse/Spcs2/Spcs2-bioinformatics/RESULTS.md
      supporting_text: Selected A0A140LHW5 has 74 residues; reference Q9CYN2 has 226 residues.
- source_method: ProtNLM2
  source_version: UniProt API snapshot 2026-09-10
  source_reference_id: file:mouse/Spcs2/Spcs2-protnlm-source.json
  predicted_term:
    id: GO:0006465
    label: signal peptide processing
  predicted_term_type: GO_BP
  review:
    assessment: UNC
    confidence_score: 1
    summary: Full-length SPCS2 contributes to signal peptide processing as a component of the signal peptidase
      complex. A0A140LHW5 lacks both reference transmembrane helices and has no demonstrated complex incorporation
      or processing phenotype; the process cannot be transferred confidently to this exact product.
    supported_by:
    - *id001
    - *id002
- source_method: ProtNLM2
  source_version: UniProt API snapshot 2026-09-10
  source_reference_id: file:mouse/Spcs2/Spcs2-protnlm-source.json
  predicted_term:
    id: GO:0005787
    label: signal peptidase complex
  predicted_term_type: GO_CC
  review:
    assessment: UNC
    confidence_score: 1
    summary: Same-gene identity supports an SPCS2 origin, but the 74-residue selected sequence retains
      only the N-terminal segment of the 226-residue membrane subunit. Membership in an assembled signal
      peptidase complex requires evidence that this short product is expressed and incorporated.
    supported_by:
    - *id002
    - *id001