Existing Annotations Review

GO Term	Evidence	Action	Reason
GO:0005886 plasma membrane	IBA GO_REF:0000033	ACCEPT	Summary: plasma membrane localization Reason: SRC is a soluble non-receptor kinase recruited to cytosolic, membrane, junctional, caveolar, and focal-adhesion signaling assemblies.
GO:0005102 signaling receptor binding	IBA GO_REF:0000033	KEEP AS NON CORE	Summary: signaling receptor binding supports SRC recruitment or substrate recognition Reason: This interaction is mechanistically relevant to SRC signaling but is accessory to the core tyrosine kinase activity.
GO:0030154 cell differentiation	IBA GO_REF:0000033	KEEP AS NON CORE	Summary: cell differentiation pathway context Reason: The annotation is compatible with SRC pathway biology but represents a context-specific process, location, or phenotype rather than the core kinase function.
GO:0004715 non-membrane spanning protein tyrosine kinase activity	IBA GO_REF:0000033	ACCEPT	Summary: Core SRC non-receptor tyrosine kinase activity Reason: SRC is a non-receptor tyrosine kinase; tyrosine phosphorylation of protein substrates is its primary molecular activity. Supporting Evidence: file:mouse/Src/Src-deep-research-bioreason.md A cytoplasmic non-receptor tyrosine kinase that uses an SH3-SH2-kinase architecture to assemble with proline-rich and phosphotyrosine-containing partners file:mouse/Src/Src-deep-research-falcon.md Falcon synthesis supports Src as a membrane-associated non-receptor tyrosine kinase with SH3, SH2, and catalytic kinase domains.
GO:0007155 cell adhesion	IBA GO_REF:0000033	KEEP AS NON CORE	Summary: cell adhesion pathway context Reason: The annotation is compatible with SRC pathway biology but represents a context-specific process, location, or phenotype rather than the core kinase function.
GO:0007173 epidermal growth factor receptor signaling pathway	IBA GO_REF:0000033	KEEP AS NON CORE	Summary: epidermal growth factor receptor signaling pathway pathway context Reason: The annotation is compatible with SRC pathway biology but represents a context-specific process, location, or phenotype rather than the core kinase function.
GO:0050847 progesterone receptor signaling pathway	IBA GO_REF:0000033	KEEP AS NON CORE	Summary: progesterone receptor signaling pathway pathway context Reason: The annotation is compatible with SRC pathway biology but represents a context-specific process, location, or phenotype rather than the core kinase function.
GO:2001237 negative regulation of extrinsic apoptotic signaling pathway	IBA GO_REF:0000033	KEEP AS NON CORE	Summary: negative regulation of extrinsic apoptotic signaling pathway pathway context Reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather than SRC core molecular activity.
GO:2001243 negative regulation of intrinsic apoptotic signaling pathway	IBA GO_REF:0000033	KEEP AS NON CORE	Summary: negative regulation of intrinsic apoptotic signaling pathway pathway context Reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather than SRC core molecular activity.
GO:0004672 protein kinase activity	IEA GO_REF:0000120	MARK AS OVER ANNOTATED	Summary: protein kinase activity is less specific than SRC tyrosine kinase activity Reason: The annotation is directionally correct but over-broad because more specific SRC tyrosine kinase terms are present.
GO:0004713 protein tyrosine kinase activity	IEA GO_REF:0000120	ACCEPT	Summary: Core SRC non-receptor tyrosine kinase activity Reason: SRC is a non-receptor tyrosine kinase; tyrosine phosphorylation of protein substrates is its primary molecular activity.
GO:0004715 non-membrane spanning protein tyrosine kinase activity	IEA GO_REF:0000120	ACCEPT	Summary: Core SRC non-receptor tyrosine kinase activity Reason: SRC is a non-receptor tyrosine kinase; tyrosine phosphorylation of protein substrates is its primary molecular activity. Supporting Evidence: file:mouse/Src/Src-deep-research-bioreason.md A cytoplasmic non-receptor tyrosine kinase that uses an SH3-SH2-kinase architecture to assemble with proline-rich and phosphotyrosine-containing partners
GO:0005524 ATP binding	IEA GO_REF:0000002	KEEP AS NON CORE	Summary: ATP binding supports SRC recruitment or substrate recognition Reason: This interaction is mechanistically relevant to SRC signaling but is accessory to the core tyrosine kinase activity.
GO:0005634 nucleus	IEA GO_REF:0000044	KEEP AS NON CORE	Summary: nucleus pathway context Reason: The annotation is compatible with SRC pathway biology but represents a context-specific process, location, or phenotype rather than the core kinase function.
GO:0005743 mitochondrial inner membrane	IEA GO_REF:0000120	KEEP AS NON CORE	Summary: mitochondrial inner membrane pathway context Reason: The annotation is compatible with SRC pathway biology but represents a context-specific process, location, or phenotype rather than the core kinase function.
GO:0005856 cytoskeleton	IEA GO_REF:0000044	KEEP AS NON CORE	Summary: cytoskeleton pathway context Reason: The annotation is compatible with SRC pathway biology but represents a context-specific process, location, or phenotype rather than the core kinase function.
GO:0005886 plasma membrane	IEA GO_REF:0000120	ACCEPT	Summary: plasma membrane localization Reason: SRC is a soluble non-receptor kinase recruited to cytosolic, membrane, junctional, caveolar, and focal-adhesion signaling assemblies.
GO:0005925 focal adhesion	IEA GO_REF:0000044	ACCEPT	Summary: focal adhesion localization Reason: SRC is a soluble non-receptor kinase recruited to cytosolic, membrane, junctional, caveolar, and focal-adhesion signaling assemblies.
GO:0007155 cell adhesion	IEA GO_REF:0000117	KEEP AS NON CORE	Summary: cell adhesion pathway context Reason: The annotation is compatible with SRC pathway biology but represents a context-specific process, location, or phenotype rather than the core kinase function.
GO:0007276 gamete generation	IEA GO_REF:0000117	KEEP AS NON CORE	Summary: gamete generation pathway context Reason: The annotation is compatible with SRC pathway biology but represents a context-specific process, location, or phenotype rather than the core kinase function.
GO:0010628 positive regulation of gene expression	IEA GO_REF:0000117	KEEP AS NON CORE	Summary: positive regulation of gene expression pathway context Reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather than SRC core molecular activity.
GO:0036120 cellular response to platelet-derived growth factor stimulus	IEA GO_REF:0000117	KEEP AS NON CORE	Summary: cellular response to platelet-derived growth factor stimulus pathway context Reason: This term captures a stimulus or pathway context in which SRC can act, not the core SRC molecular activity.
GO:0048471 perinuclear region of cytoplasm	IEA GO_REF:0000120	KEEP AS NON CORE	Summary: perinuclear region of cytoplasm pathway context Reason: The annotation is compatible with SRC pathway biology but represents a context-specific process, location, or phenotype rather than the core kinase function.
GO:0070161 anchoring junction	IEA GO_REF:0000044	KEEP AS NON CORE	Summary: anchoring junction pathway context Reason: The annotation is compatible with SRC pathway biology but represents a context-specific process, location, or phenotype rather than the core kinase function.
GO:0070301 cellular response to hydrogen peroxide	IEA GO_REF:0000117	KEEP AS NON CORE	Summary: cellular response to hydrogen peroxide pathway context Reason: This term captures a stimulus or pathway context in which SRC can act, not the core SRC molecular activity.
GO:0070374 positive regulation of ERK1 and ERK2 cascade	IEA GO_REF:0000117	KEEP AS NON CORE	Summary: positive regulation of ERK1 and ERK2 cascade pathway context Reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather than SRC core molecular activity.
GO:1903829 positive regulation of protein localization	IEA GO_REF:0000117	KEEP AS NON CORE	Summary: positive regulation of protein localization pathway context Reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather than SRC core molecular activity.
GO:1904385 cellular response to angiotensin	IEA GO_REF:0000117	KEEP AS NON CORE	Summary: cellular response to angiotensin pathway context Reason: This term captures a stimulus or pathway context in which SRC can act, not the core SRC molecular activity.
GO:0005515 protein binding	IPI PMID:10220404 Identification of profilin and src homology 3 domains as bin...	MARK AS OVER ANNOTATED	Summary: Generic protein binding Reason: Protein binding does not identify a specific SRC substrate-recognition mode or catalytic activity and is less informative than SH2/SH3/domain-specific terms.
GO:0005515 protein binding	IPI PMID:16267818 A proteomics strategy for the enrichment of receptor-associa...	MARK AS OVER ANNOTATED	Summary: Generic protein binding Reason: Protein binding does not identify a specific SRC substrate-recognition mode or catalytic activity and is less informative than SH2/SH3/domain-specific terms.
GO:0005515 protein binding	IPI PMID:16298995 EphB receptors regulate dendritic spine morphogenesis throug...	MARK AS OVER ANNOTATED	Summary: Generic protein binding Reason: Protein binding does not identify a specific SRC substrate-recognition mode or catalytic activity and is less informative than SH2/SH3/domain-specific terms.
GO:0005515 protein binding	IPI PMID:16407161 Role of Src in C3 transient receptor potential channel funct...	MARK AS OVER ANNOTATED	Summary: Generic protein binding Reason: Protein binding does not identify a specific SRC substrate-recognition mode or catalytic activity and is less informative than SH2/SH3/domain-specific terms.
GO:0005515 protein binding	IPI PMID:16990796 PSD-95 is a negative regulator of the tyrosine kinase Src in...	MARK AS OVER ANNOTATED	Summary: Generic protein binding Reason: Protein binding does not identify a specific SRC substrate-recognition mode or catalytic activity and is less informative than SH2/SH3/domain-specific terms.
GO:0005515 protein binding	IPI PMID:18289379 Dramatic inhibition of osteoclast sealing ring formation and...	MARK AS OVER ANNOTATED	Summary: Generic protein binding Reason: Protein binding does not identify a specific SRC substrate-recognition mode or catalytic activity and is less informative than SH2/SH3/domain-specific terms.
GO:0005515 protein binding	IPI PMID:18560762 Mobility of TOAC spin-labelled peptides binding to the Src S...	MARK AS OVER ANNOTATED	Summary: Generic protein binding Reason: Protein binding does not identify a specific SRC substrate-recognition mode or catalytic activity and is less informative than SH2/SH3/domain-specific terms.
GO:0005515 protein binding	IPI PMID:19122674 Deficiency of a beta-arrestin-2 signal complex contributes t...	MARK AS OVER ANNOTATED	Summary: Generic protein binding Reason: Protein binding does not identify a specific SRC substrate-recognition mode or catalytic activity and is less informative than SH2/SH3/domain-specific terms.
GO:0005515 protein binding	IPI PMID:21199370 Roles of the SH2 and SH3 domains in the regulation of neuron...	MARK AS OVER ANNOTATED	Summary: Generic protein binding Reason: Protein binding does not identify a specific SRC substrate-recognition mode or catalytic activity and is less informative than SH2/SH3/domain-specific terms.
GO:0005515 protein binding	IPI PMID:21266176 Linker length dependent binding of a focal adhesion kinase d...	MARK AS OVER ANNOTATED	Summary: Generic protein binding Reason: Protein binding does not identify a specific SRC substrate-recognition mode or catalytic activity and is less informative than SH2/SH3/domain-specific terms.
GO:0005515 protein binding	IPI PMID:21282606 Nε-lysine acetylation determines dissociation from GAP junct...	MARK AS OVER ANNOTATED	Summary: Generic protein binding Reason: Protein binding does not identify a specific SRC substrate-recognition mode or catalytic activity and is less informative than SH2/SH3/domain-specific terms.
GO:0005515 protein binding	IPI PMID:22439932 NKX2-1/TITF1/TTF-1-Induced ROR1 is required to sustain EGFR ...	MARK AS OVER ANNOTATED	Summary: Generic protein binding Reason: Protein binding does not identify a specific SRC substrate-recognition mode or catalytic activity and is less informative than SH2/SH3/domain-specific terms.
GO:0005515 protein binding	IPI PMID:28054552 c-Src phosphorylation and activation of hexokinase promotes ...	MARK AS OVER ANNOTATED	Summary: Generic protein binding Reason: Protein binding does not identify a specific SRC substrate-recognition mode or catalytic activity and is less informative than SH2/SH3/domain-specific terms.
GO:0005515 protein binding	IPI PMID:7518772 Phosphorylation of receptor protein-tyrosine phosphatase alp...	MARK AS OVER ANNOTATED	Summary: Generic protein binding Reason: Protein binding does not identify a specific SRC substrate-recognition mode or catalytic activity and is less informative than SH2/SH3/domain-specific terms.
GO:0000122 negative regulation of transcription by RNA polymerase II	IEA GO_REF:0000107	KEEP AS NON CORE	Summary: negative regulation of transcription by RNA polymerase II pathway context Reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather than SRC core molecular activity.
GO:0005102 signaling receptor binding	IEA GO_REF:0000107	KEEP AS NON CORE	Summary: signaling receptor binding supports SRC recruitment or substrate recognition Reason: This interaction is mechanistically relevant to SRC signaling but is accessory to the core tyrosine kinase activity.
GO:0005178 integrin binding	IEA GO_REF:0000107	KEEP AS NON CORE	Summary: integrin binding pathway context Reason: The annotation is compatible with SRC pathway biology but represents a context-specific process, location, or phenotype rather than the core kinase function.
GO:0005737 cytoplasm	IEA GO_REF:0000120	ACCEPT	Summary: cytoplasm localization Reason: SRC is a soluble non-receptor kinase recruited to cytosolic, membrane, junctional, caveolar, and focal-adhesion signaling assemblies.
GO:0005739 mitochondrion	IEA GO_REF:0000107	KEEP AS NON CORE	Summary: mitochondrion pathway context Reason: The annotation is compatible with SRC pathway biology but represents a context-specific process, location, or phenotype rather than the core kinase function.
GO:0005764 lysosome	IEA GO_REF:0000107	KEEP AS NON CORE	Summary: lysosome pathway context Reason: The annotation is compatible with SRC pathway biology but represents a context-specific process, location, or phenotype rather than the core kinase function.
GO:0005770 late endosome	IEA GO_REF:0000107	KEEP AS NON CORE	Summary: late endosome pathway context Reason: The annotation is compatible with SRC pathway biology but represents a context-specific process, location, or phenotype rather than the core kinase function.
GO:0005829 cytosol	IEA GO_REF:0000107	ACCEPT	Summary: cytosol localization Reason: SRC is a soluble non-receptor kinase recruited to cytosolic, membrane, junctional, caveolar, and focal-adhesion signaling assemblies.
GO:0005901 caveola	IEA GO_REF:0000120	ACCEPT	Summary: caveola localization Reason: SRC is a soluble non-receptor kinase recruited to cytosolic, membrane, junctional, caveolar, and focal-adhesion signaling assemblies.
GO:0005911 cell-cell junction	IEA GO_REF:0000107	ACCEPT	Summary: cell-cell junction localization Reason: SRC is a soluble non-receptor kinase recruited to cytosolic, membrane, junctional, caveolar, and focal-adhesion signaling assemblies.
GO:0007179 transforming growth factor beta receptor signaling pathway	IEA GO_REF:0000107	KEEP AS NON CORE	Summary: transforming growth factor beta receptor signaling pathway pathway context Reason: The annotation is compatible with SRC pathway biology but represents a context-specific process, location, or phenotype rather than the core kinase function.
GO:0007229 integrin-mediated signaling pathway	IEA GO_REF:0000107	KEEP AS NON CORE	Summary: integrin-mediated signaling pathway pathway context Reason: The annotation is compatible with SRC pathway biology but represents a context-specific process, location, or phenotype rather than the core kinase function.
GO:0010632 regulation of epithelial cell migration	IEA GO_REF:0000107	KEEP AS NON CORE	Summary: regulation of epithelial cell migration pathway context Reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather than SRC core molecular activity.
GO:0010634 positive regulation of epithelial cell migration	IEA GO_REF:0000107	KEEP AS NON CORE	Summary: positive regulation of epithelial cell migration pathway context Reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather than SRC core molecular activity.
GO:0016004 phospholipase activator activity	IEA GO_REF:0000107	KEEP AS NON CORE	Summary: phospholipase activator activity pathway context Reason: The annotation is compatible with SRC pathway biology but represents a context-specific process, location, or phenotype rather than the core kinase function.
GO:0019899 enzyme binding	IEA GO_REF:0000107	MARK AS OVER ANNOTATED	Summary: Generic enzyme binding annotation Reason: The term is too broad or peripheral to capture SRC function when more specific tyrosine kinase and signaling annotations are available.
GO:0020037 heme binding	IEA GO_REF:0000107	MARK AS OVER ANNOTATED	Summary: Generic heme binding annotation Reason: The term is too broad or peripheral to capture SRC function when more specific tyrosine kinase and signaling annotations are available.
GO:0022407 regulation of cell-cell adhesion	IEA GO_REF:0000107	KEEP AS NON CORE	Summary: regulation of cell-cell adhesion pathway context Reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather than SRC core molecular activity.
GO:0030054 cell junction	IEA GO_REF:0000107	ACCEPT	Summary: cell junction localization Reason: SRC is a soluble non-receptor kinase recruited to cytosolic, membrane, junctional, caveolar, and focal-adhesion signaling assemblies.
GO:0031333 negative regulation of protein-containing complex assembly	IEA GO_REF:0000107	KEEP AS NON CORE	Summary: negative regulation of protein-containing complex assembly pathway context Reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather than SRC core molecular activity.
GO:0034139 regulation of toll-like receptor 3 signaling pathway	IEA GO_REF:0000107	KEEP AS NON CORE	Summary: regulation of toll-like receptor 3 signaling pathway pathway context Reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather than SRC core molecular activity.
GO:0034614 cellular response to reactive oxygen species	IEA GO_REF:0000107	KEEP AS NON CORE	Summary: cellular response to reactive oxygen species pathway context Reason: This term captures a stimulus or pathway context in which SRC can act, not the core SRC molecular activity.
GO:0035306 positive regulation of dephosphorylation	IEA GO_REF:0000107	KEEP AS NON CORE	Summary: positive regulation of dephosphorylation pathway context Reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather than SRC core molecular activity.
GO:0035331 negative regulation of hippo signaling	IEA GO_REF:0000107	KEEP AS NON CORE	Summary: negative regulation of hippo signaling pathway context Reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather than SRC core molecular activity.
GO:0035556 intracellular signal transduction	IEA GO_REF:0000107	ACCEPT	Summary: SRC intracellular signaling function Reason: SRC acts near activated receptors and adhesion complexes to propagate tyrosine-phosphorylation-dependent intracellular signaling.
GO:0042169 SH2 domain binding	IEA GO_REF:0000107	KEEP AS NON CORE	Summary: SH2 domain binding supports SRC recruitment or substrate recognition Reason: This interaction is mechanistically relevant to SRC signaling but is accessory to the core tyrosine kinase activity.
GO:0043066 negative regulation of apoptotic process	IEA GO_REF:0000107	KEEP AS NON CORE	Summary: negative regulation of apoptotic process pathway context Reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather than SRC core molecular activity.
GO:0043149 stress fiber assembly	IEA GO_REF:0000107	KEEP AS NON CORE	Summary: stress fiber assembly pathway context Reason: The annotation is compatible with SRC pathway biology but represents a context-specific process, location, or phenotype rather than the core kinase function.
GO:0043274 phospholipase binding	IEA GO_REF:0000107	KEEP AS NON CORE	Summary: phospholipase binding pathway context Reason: The annotation is compatible with SRC pathway biology but represents a context-specific process, location, or phenotype rather than the core kinase function.
GO:0044325 transmembrane transporter binding	IEA GO_REF:0000107	KEEP AS NON CORE	Summary: transmembrane transporter binding pathway context Reason: The annotation is compatible with SRC pathway biology but represents a context-specific process, location, or phenotype rather than the core kinase function.
GO:0045747 positive regulation of Notch signaling pathway	IEA GO_REF:0000107	KEEP AS NON CORE	Summary: positive regulation of Notch signaling pathway pathway context Reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather than SRC core molecular activity.
GO:0046875 ephrin receptor binding	IEA GO_REF:0000107	KEEP AS NON CORE	Summary: ephrin receptor binding supports SRC recruitment or substrate recognition Reason: This interaction is mechanistically relevant to SRC signaling but is accessory to the core tyrosine kinase activity.
GO:0048041 focal adhesion assembly	IEA GO_REF:0000107	KEEP AS NON CORE	Summary: focal adhesion assembly pathway context Reason: The annotation is compatible with SRC pathway biology but represents a context-specific process, location, or phenotype rather than the core kinase function.
GO:0051057 positive regulation of small GTPase mediated signal transduction	IEA GO_REF:0000120	KEEP AS NON CORE	Summary: positive regulation of small GTPase mediated signal transduction pathway context Reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather than SRC core molecular activity.
GO:0051219 phosphoprotein binding	IEA GO_REF:0000107	KEEP AS NON CORE	Summary: phosphoprotein binding supports SRC recruitment or substrate recognition Reason: This interaction is mechanistically relevant to SRC signaling but is accessory to the core tyrosine kinase activity.
GO:0051897 positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction	IEA GO_REF:0000107	KEEP AS NON CORE	Summary: positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction pathway context Reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather than SRC core molecular activity.
GO:0051902 negative regulation of mitochondrial depolarization	IEA GO_REF:0000107	KEEP AS NON CORE	Summary: negative regulation of mitochondrial depolarization pathway context Reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather than SRC core molecular activity.
GO:0060576 intestinal epithelial cell development	IEA GO_REF:0000107	KEEP AS NON CORE	Summary: intestinal epithelial cell development pathway context Reason: The annotation is compatible with SRC pathway biology but represents a context-specific process, location, or phenotype rather than the core kinase function.
GO:0070102 interleukin-6-mediated signaling pathway	IEA GO_REF:0000107	KEEP AS NON CORE	Summary: interleukin-6-mediated signaling pathway pathway context Reason: The annotation is compatible with SRC pathway biology but represents a context-specific process, location, or phenotype rather than the core kinase function.
GO:0070555 response to interleukin-1	IEA GO_REF:0000107	KEEP AS NON CORE	Summary: response to interleukin-1 pathway context Reason: This term captures a stimulus or pathway context in which SRC can act, not the core SRC molecular activity.
GO:0070700 BMP receptor binding	IEA GO_REF:0000107	KEEP AS NON CORE	Summary: BMP receptor binding pathway context Reason: The annotation is compatible with SRC pathway biology but represents a context-specific process, location, or phenotype rather than the core kinase function.
GO:0097110 scaffold protein binding	IEA GO_REF:0000107	KEEP AS NON CORE	Summary: scaffold protein binding supports SRC recruitment or substrate recognition Reason: This interaction is mechanistically relevant to SRC signaling but is accessory to the core tyrosine kinase activity.
GO:1902564 negative regulation of neutrophil activation	IEA GO_REF:0000107	KEEP AS NON CORE	Summary: negative regulation of neutrophil activation pathway context Reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather than SRC core molecular activity.
GO:1904263 positive regulation of TORC1 signaling	IEA GO_REF:0000107	KEEP AS NON CORE	Summary: positive regulation of TORC1 signaling pathway context Reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather than SRC core molecular activity.
GO:2000394 positive regulation of lamellipodium morphogenesis	IEA GO_REF:0000107	KEEP AS NON CORE	Summary: positive regulation of lamellipodium morphogenesis pathway context Reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather than SRC core molecular activity.
GO:2000641 regulation of early endosome to late endosome transport	IEA GO_REF:0000107	KEEP AS NON CORE	Summary: regulation of early endosome to late endosome transport pathway context Reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather than SRC core molecular activity.
GO:2000811 negative regulation of anoikis	IEA GO_REF:0000107	KEEP AS NON CORE	Summary: negative regulation of anoikis pathway context Reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather than SRC core molecular activity.
GO:2001237 negative regulation of extrinsic apoptotic signaling pathway	IEA GO_REF:0000107	KEEP AS NON CORE	Summary: negative regulation of extrinsic apoptotic signaling pathway pathway context Reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather than SRC core molecular activity.
GO:2001243 negative regulation of intrinsic apoptotic signaling pathway	IEA GO_REF:0000107	KEEP AS NON CORE	Summary: negative regulation of intrinsic apoptotic signaling pathway pathway context Reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather than SRC core molecular activity.
GO:2001286 regulation of caveolin-mediated endocytosis	IEA GO_REF:0000107	KEEP AS NON CORE	Summary: regulation of caveolin-mediated endocytosis pathway context Reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather than SRC core molecular activity.
GO:0000122 negative regulation of transcription by RNA polymerase II	ISO GO_REF:0000119	KEEP AS NON CORE	Summary: negative regulation of transcription by RNA polymerase II pathway context Reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather than SRC core molecular activity.
GO:0001545 primary ovarian follicle growth	ISO GO_REF:0000096	KEEP AS NON CORE	Summary: primary ovarian follicle growth pathway context Reason: The annotation is compatible with SRC pathway biology but represents a context-specific process, location, or phenotype rather than the core kinase function.
GO:0001819 positive regulation of cytokine production	ISO GO_REF:0000096	KEEP AS NON CORE	Summary: positive regulation of cytokine production pathway context Reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather than SRC core molecular activity.
GO:0004672 protein kinase activity	ISO GO_REF:0000119	MARK AS OVER ANNOTATED	Summary: protein kinase activity is less specific than SRC tyrosine kinase activity Reason: The annotation is directionally correct but over-broad because more specific SRC tyrosine kinase terms are present.
GO:0004713 protein tyrosine kinase activity	ISO GO_REF:0000119	ACCEPT	Summary: Core SRC non-receptor tyrosine kinase activity Reason: SRC is a non-receptor tyrosine kinase; tyrosine phosphorylation of protein substrates is its primary molecular activity.
GO:0004715 non-membrane spanning protein tyrosine kinase activity	ISO GO_REF:0000119	ACCEPT	Summary: Core SRC non-receptor tyrosine kinase activity Reason: SRC is a non-receptor tyrosine kinase; tyrosine phosphorylation of protein substrates is its primary molecular activity. Supporting Evidence: file:mouse/Src/Src-deep-research-bioreason.md A cytoplasmic non-receptor tyrosine kinase that uses an SH3-SH2-kinase architecture to assemble with proline-rich and phosphotyrosine-containing partners
GO:0005102 signaling receptor binding	ISO GO_REF:0000119	KEEP AS NON CORE	Summary: signaling receptor binding supports SRC recruitment or substrate recognition Reason: This interaction is mechanistically relevant to SRC signaling but is accessory to the core tyrosine kinase activity.
GO:0005178 integrin binding	ISO GO_REF:0000119	KEEP AS NON CORE	Summary: integrin binding pathway context Reason: The annotation is compatible with SRC pathway biology but represents a context-specific process, location, or phenotype rather than the core kinase function.
GO:0005737 cytoplasm	ISO GO_REF:0000119	ACCEPT	Summary: cytoplasm localization Reason: SRC is a soluble non-receptor kinase recruited to cytosolic, membrane, junctional, caveolar, and focal-adhesion signaling assemblies.
GO:0005739 mitochondrion	ISO GO_REF:0000119	KEEP AS NON CORE	Summary: mitochondrion pathway context Reason: The annotation is compatible with SRC pathway biology but represents a context-specific process, location, or phenotype rather than the core kinase function.
GO:0005743 mitochondrial inner membrane	ISO GO_REF:0000119	KEEP AS NON CORE	Summary: mitochondrial inner membrane pathway context Reason: The annotation is compatible with SRC pathway biology but represents a context-specific process, location, or phenotype rather than the core kinase function.
GO:0005764 lysosome	ISO GO_REF:0000119	KEEP AS NON CORE	Summary: lysosome pathway context Reason: The annotation is compatible with SRC pathway biology but represents a context-specific process, location, or phenotype rather than the core kinase function.
GO:0005770 late endosome	ISO GO_REF:0000119	KEEP AS NON CORE	Summary: late endosome pathway context Reason: The annotation is compatible with SRC pathway biology but represents a context-specific process, location, or phenotype rather than the core kinase function.
GO:0005829 cytosol	ISO GO_REF:0000119	ACCEPT	Summary: cytosol localization Reason: SRC is a soluble non-receptor kinase recruited to cytosolic, membrane, junctional, caveolar, and focal-adhesion signaling assemblies.
GO:0005886 plasma membrane	ISO GO_REF:0000119	ACCEPT	Summary: plasma membrane localization Reason: SRC is a soluble non-receptor kinase recruited to cytosolic, membrane, junctional, caveolar, and focal-adhesion signaling assemblies.
GO:0005901 caveola	ISO GO_REF:0000119	ACCEPT	Summary: caveola localization Reason: SRC is a soluble non-receptor kinase recruited to cytosolic, membrane, junctional, caveolar, and focal-adhesion signaling assemblies.
GO:0005911 cell-cell junction	ISO GO_REF:0000119	ACCEPT	Summary: cell-cell junction localization Reason: SRC is a soluble non-receptor kinase recruited to cytosolic, membrane, junctional, caveolar, and focal-adhesion signaling assemblies.
GO:0007179 transforming growth factor beta receptor signaling pathway	ISO GO_REF:0000119	KEEP AS NON CORE	Summary: transforming growth factor beta receptor signaling pathway pathway context Reason: The annotation is compatible with SRC pathway biology but represents a context-specific process, location, or phenotype rather than the core kinase function.
GO:0007229 integrin-mediated signaling pathway	ISO GO_REF:0000119	KEEP AS NON CORE	Summary: integrin-mediated signaling pathway pathway context Reason: The annotation is compatible with SRC pathway biology but represents a context-specific process, location, or phenotype rather than the core kinase function.
GO:0008284 positive regulation of cell population proliferation	ISO GO_REF:0000096	KEEP AS NON CORE	Summary: positive regulation of cell population proliferation pathway context Reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather than SRC core molecular activity.
GO:0010628 positive regulation of gene expression	ISO GO_REF:0000096	KEEP AS NON CORE	Summary: positive regulation of gene expression pathway context Reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather than SRC core molecular activity.
GO:0010629 negative regulation of gene expression	ISO GO_REF:0000096	KEEP AS NON CORE	Summary: negative regulation of gene expression pathway context Reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather than SRC core molecular activity.
GO:0010632 regulation of epithelial cell migration	ISO GO_REF:0000119	KEEP AS NON CORE	Summary: regulation of epithelial cell migration pathway context Reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather than SRC core molecular activity.
GO:0010634 positive regulation of epithelial cell migration	ISO GO_REF:0000119	KEEP AS NON CORE	Summary: positive regulation of epithelial cell migration pathway context Reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather than SRC core molecular activity.
GO:0010907 positive regulation of glucose metabolic process	ISO GO_REF:0000096	KEEP AS NON CORE	Summary: positive regulation of glucose metabolic process pathway context Reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather than SRC core molecular activity.
GO:0014911 positive regulation of smooth muscle cell migration	ISO GO_REF:0000096	KEEP AS NON CORE	Summary: positive regulation of smooth muscle cell migration pathway context Reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather than SRC core molecular activity.
GO:0016004 phospholipase activator activity	ISO GO_REF:0000119	KEEP AS NON CORE	Summary: phospholipase activator activity pathway context Reason: The annotation is compatible with SRC pathway biology but represents a context-specific process, location, or phenotype rather than the core kinase function.
GO:0019899 enzyme binding	ISO GO_REF:0000119	MARK AS OVER ANNOTATED	Summary: Generic enzyme binding annotation Reason: The term is too broad or peripheral to capture SRC function when more specific tyrosine kinase and signaling annotations are available.
GO:0020037 heme binding	ISO GO_REF:0000119	MARK AS OVER ANNOTATED	Summary: Generic heme binding annotation Reason: The term is too broad or peripheral to capture SRC function when more specific tyrosine kinase and signaling annotations are available.
GO:0022407 regulation of cell-cell adhesion	ISO GO_REF:0000119	KEEP AS NON CORE	Summary: regulation of cell-cell adhesion pathway context Reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather than SRC core molecular activity.
GO:0030054 cell junction	ISO GO_REF:0000119	ACCEPT	Summary: cell junction localization Reason: SRC is a soluble non-receptor kinase recruited to cytosolic, membrane, junctional, caveolar, and focal-adhesion signaling assemblies.
GO:0030335 positive regulation of cell migration	ISO GO_REF:0000096	KEEP AS NON CORE	Summary: positive regulation of cell migration pathway context Reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather than SRC core molecular activity.
GO:0031333 negative regulation of protein-containing complex assembly	ISO GO_REF:0000119	KEEP AS NON CORE	Summary: negative regulation of protein-containing complex assembly pathway context Reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather than SRC core molecular activity.
GO:0032205 negative regulation of telomere maintenance	ISO GO_REF:0000119	KEEP AS NON CORE	Summary: negative regulation of telomere maintenance pathway context Reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather than SRC core molecular activity.
GO:0034139 regulation of toll-like receptor 3 signaling pathway	ISO GO_REF:0000119	KEEP AS NON CORE	Summary: regulation of toll-like receptor 3 signaling pathway pathway context Reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather than SRC core molecular activity.
GO:0034614 cellular response to reactive oxygen species	ISO GO_REF:0000119	KEEP AS NON CORE	Summary: cellular response to reactive oxygen species pathway context Reason: This term captures a stimulus or pathway context in which SRC can act, not the core SRC molecular activity.
GO:0035306 positive regulation of dephosphorylation	ISO GO_REF:0000119	KEEP AS NON CORE	Summary: positive regulation of dephosphorylation pathway context Reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather than SRC core molecular activity.
GO:0035331 negative regulation of hippo signaling	ISO GO_REF:0000119	KEEP AS NON CORE	Summary: negative regulation of hippo signaling pathway context Reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather than SRC core molecular activity.
GO:0035556 intracellular signal transduction	ISO GO_REF:0000119	ACCEPT	Summary: SRC intracellular signaling function Reason: SRC acts near activated receptors and adhesion complexes to propagate tyrosine-phosphorylation-dependent intracellular signaling.
GO:0038083 peptidyl-tyrosine autophosphorylation	ISO GO_REF:0000096	ACCEPT	Summary: Direct SRC tyrosine phosphorylation activity Reason: This term directly describes the catalytic output of SRC kinase activity on protein tyrosine residues.
GO:0042169 SH2 domain binding	ISO GO_REF:0000119	KEEP AS NON CORE	Summary: SH2 domain binding supports SRC recruitment or substrate recognition Reason: This interaction is mechanistically relevant to SRC signaling but is accessory to the core tyrosine kinase activity.
GO:0042311 vasodilation	ISO GO_REF:0000119	KEEP AS NON CORE	Summary: vasodilation pathway context Reason: The annotation is compatible with SRC pathway biology but represents a context-specific process, location, or phenotype rather than the core kinase function.
GO:0043065 positive regulation of apoptotic process	ISO GO_REF:0000096	KEEP AS NON CORE	Summary: positive regulation of apoptotic process pathway context Reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather than SRC core molecular activity.
GO:0043066 negative regulation of apoptotic process	ISO GO_REF:0000119	KEEP AS NON CORE	Summary: negative regulation of apoptotic process pathway context Reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather than SRC core molecular activity.
GO:0043149 stress fiber assembly	ISO GO_REF:0000119	KEEP AS NON CORE	Summary: stress fiber assembly pathway context Reason: The annotation is compatible with SRC pathway biology but represents a context-specific process, location, or phenotype rather than the core kinase function.
GO:0043274 phospholipase binding	ISO GO_REF:0000119	KEEP AS NON CORE	Summary: phospholipase binding pathway context Reason: The annotation is compatible with SRC pathway biology but represents a context-specific process, location, or phenotype rather than the core kinase function.
GO:0043406 positive regulation of MAP kinase activity	ISO GO_REF:0000096	KEEP AS NON CORE	Summary: positive regulation of MAP kinase activity pathway context Reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather than SRC core molecular activity.
GO:0044325 transmembrane transporter binding	ISO GO_REF:0000119	KEEP AS NON CORE	Summary: transmembrane transporter binding pathway context Reason: The annotation is compatible with SRC pathway biology but represents a context-specific process, location, or phenotype rather than the core kinase function.
GO:0045056 transcytosis	ISO GO_REF:0000096	KEEP AS NON CORE	Summary: transcytosis pathway context Reason: The annotation is compatible with SRC pathway biology but represents a context-specific process, location, or phenotype rather than the core kinase function.
GO:0045747 positive regulation of Notch signaling pathway	ISO GO_REF:0000119	KEEP AS NON CORE	Summary: positive regulation of Notch signaling pathway pathway context Reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather than SRC core molecular activity.
GO:0045780 positive regulation of bone resorption	ISO GO_REF:0000096	KEEP AS NON CORE	Summary: positive regulation of bone resorption pathway context Reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather than SRC core molecular activity.
GO:0045785 positive regulation of cell adhesion	ISO GO_REF:0000096	KEEP AS NON CORE	Summary: positive regulation of cell adhesion pathway context Reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather than SRC core molecular activity.
GO:0045793 positive regulation of cell size	ISO GO_REF:0000096	KEEP AS NON CORE	Summary: positive regulation of cell size pathway context Reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather than SRC core molecular activity.
GO:0046579 positive regulation of Ras protein signal transduction	ISO GO_REF:0000096	KEEP AS NON CORE	Summary: positive regulation of Ras protein signal transduction pathway context Reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather than SRC core molecular activity.
GO:0046628 positive regulation of insulin receptor signaling pathway	ISO GO_REF:0000096	KEEP AS NON CORE	Summary: positive regulation of insulin receptor signaling pathway pathway context Reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather than SRC core molecular activity.
GO:0046875 ephrin receptor binding	ISO GO_REF:0000119	KEEP AS NON CORE	Summary: ephrin receptor binding supports SRC recruitment or substrate recognition Reason: This interaction is mechanistically relevant to SRC signaling but is accessory to the core tyrosine kinase activity.
GO:0048011 neurotrophin TRK receptor signaling pathway	ISO GO_REF:0000096	KEEP AS NON CORE	Summary: neurotrophin TRK receptor signaling pathway pathway context Reason: The annotation is compatible with SRC pathway biology but represents a context-specific process, location, or phenotype rather than the core kinase function.
GO:0048041 focal adhesion assembly	ISO GO_REF:0000119	KEEP AS NON CORE	Summary: focal adhesion assembly pathway context Reason: The annotation is compatible with SRC pathway biology but represents a context-specific process, location, or phenotype rather than the core kinase function.
GO:0048471 perinuclear region of cytoplasm	ISO GO_REF:0000119	KEEP AS NON CORE	Summary: perinuclear region of cytoplasm pathway context Reason: The annotation is compatible with SRC pathway biology but represents a context-specific process, location, or phenotype rather than the core kinase function.
GO:0050731 positive regulation of peptidyl-tyrosine phosphorylation	ISO GO_REF:0000096	KEEP AS NON CORE	Summary: positive regulation of peptidyl-tyrosine phosphorylation pathway context Reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather than SRC core molecular activity.
GO:0050839 cell adhesion molecule binding	ISO GO_REF:0000096	KEEP AS NON CORE	Summary: cell adhesion molecule binding pathway context Reason: The annotation is compatible with SRC pathway biology but represents a context-specific process, location, or phenotype rather than the core kinase function.
GO:0050847 progesterone receptor signaling pathway	ISO GO_REF:0000096	KEEP AS NON CORE	Summary: progesterone receptor signaling pathway pathway context Reason: The annotation is compatible with SRC pathway biology but represents a context-specific process, location, or phenotype rather than the core kinase function.
GO:0051057 positive regulation of small GTPase mediated signal transduction	ISO GO_REF:0000119	KEEP AS NON CORE	Summary: positive regulation of small GTPase mediated signal transduction pathway context Reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather than SRC core molecular activity.
GO:0051219 phosphoprotein binding	ISO GO_REF:0000119	KEEP AS NON CORE	Summary: phosphoprotein binding supports SRC recruitment or substrate recognition Reason: This interaction is mechanistically relevant to SRC signaling but is accessory to the core tyrosine kinase activity.
GO:0051222 positive regulation of protein transport	ISO GO_REF:0000096	KEEP AS NON CORE	Summary: positive regulation of protein transport pathway context Reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather than SRC core molecular activity.
GO:0051895 negative regulation of focal adhesion assembly	ISO GO_REF:0000096	KEEP AS NON CORE	Summary: negative regulation of focal adhesion assembly pathway context Reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather than SRC core molecular activity.
GO:0051897 positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction	ISO GO_REF:0000096	KEEP AS NON CORE	Summary: positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction pathway context Reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather than SRC core molecular activity.
GO:0051897 positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction	ISO GO_REF:0000119	KEEP AS NON CORE	Summary: positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction pathway context Reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather than SRC core molecular activity.
GO:0051902 negative regulation of mitochondrial depolarization	ISO GO_REF:0000119	KEEP AS NON CORE	Summary: negative regulation of mitochondrial depolarization pathway context Reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather than SRC core molecular activity.
GO:0060576 intestinal epithelial cell development	ISO GO_REF:0000119	KEEP AS NON CORE	Summary: intestinal epithelial cell development pathway context Reason: The annotation is compatible with SRC pathway biology but represents a context-specific process, location, or phenotype rather than the core kinase function.
GO:0070102 interleukin-6-mediated signaling pathway	ISO GO_REF:0000119	KEEP AS NON CORE	Summary: interleukin-6-mediated signaling pathway pathway context Reason: The annotation is compatible with SRC pathway biology but represents a context-specific process, location, or phenotype rather than the core kinase function.
GO:0070555 response to interleukin-1	ISO GO_REF:0000119	KEEP AS NON CORE	Summary: response to interleukin-1 pathway context Reason: This term captures a stimulus or pathway context in which SRC can act, not the core SRC molecular activity.
GO:0070700 BMP receptor binding	ISO GO_REF:0000119	KEEP AS NON CORE	Summary: BMP receptor binding pathway context Reason: The annotation is compatible with SRC pathway biology but represents a context-specific process, location, or phenotype rather than the core kinase function.
GO:0097110 scaffold protein binding	ISO GO_REF:0000119	KEEP AS NON CORE	Summary: scaffold protein binding supports SRC recruitment or substrate recognition Reason: This interaction is mechanistically relevant to SRC signaling but is accessory to the core tyrosine kinase activity.
GO:1902564 negative regulation of neutrophil activation	ISO GO_REF:0000119	KEEP AS NON CORE	Summary: negative regulation of neutrophil activation pathway context Reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather than SRC core molecular activity.
GO:1904263 positive regulation of TORC1 signaling	ISO GO_REF:0000119	KEEP AS NON CORE	Summary: positive regulation of TORC1 signaling pathway context Reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather than SRC core molecular activity.
GO:2000394 positive regulation of lamellipodium morphogenesis	ISO GO_REF:0000119	KEEP AS NON CORE	Summary: positive regulation of lamellipodium morphogenesis pathway context Reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather than SRC core molecular activity.
GO:2000641 regulation of early endosome to late endosome transport	ISO GO_REF:0000119	KEEP AS NON CORE	Summary: regulation of early endosome to late endosome transport pathway context Reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather than SRC core molecular activity.
GO:2000811 negative regulation of anoikis	ISO GO_REF:0000119	KEEP AS NON CORE	Summary: negative regulation of anoikis pathway context Reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather than SRC core molecular activity.
GO:2001237 negative regulation of extrinsic apoptotic signaling pathway	ISO GO_REF:0000119	KEEP AS NON CORE	Summary: negative regulation of extrinsic apoptotic signaling pathway pathway context Reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather than SRC core molecular activity.
GO:2001243 negative regulation of intrinsic apoptotic signaling pathway	ISO GO_REF:0000119	KEEP AS NON CORE	Summary: negative regulation of intrinsic apoptotic signaling pathway pathway context Reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather than SRC core molecular activity.
GO:2001286 regulation of caveolin-mediated endocytosis	ISO GO_REF:0000119	KEEP AS NON CORE	Summary: regulation of caveolin-mediated endocytosis pathway context Reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather than SRC core molecular activity.
GO:0030331 nuclear estrogen receptor binding	ISO GO_REF:0000096	KEEP AS NON CORE	Summary: nuclear estrogen receptor binding pathway context Reason: The annotation is compatible with SRC pathway biology but represents a context-specific process, location, or phenotype rather than the core kinase function.
GO:0030546 signaling receptor activator activity	ISO GO_REF:0000096	KEEP AS NON CORE	Summary: signaling receptor activator activity pathway context Reason: The annotation is compatible with SRC pathway biology but represents a context-specific process, location, or phenotype rather than the core kinase function.
GO:0035255 ionotropic glutamate receptor binding	ISO GO_REF:0000096	KEEP AS NON CORE	Summary: ionotropic glutamate receptor binding pathway context Reason: The annotation is compatible with SRC pathway biology but represents a context-specific process, location, or phenotype rather than the core kinase function.
GO:0044325 transmembrane transporter binding	ISO GO_REF:0000096	KEEP AS NON CORE	Summary: transmembrane transporter binding pathway context Reason: The annotation is compatible with SRC pathway biology but represents a context-specific process, location, or phenotype rather than the core kinase function.
GO:0004713 protein tyrosine kinase activity	ISO GO_REF:0000096	ACCEPT	Summary: Core SRC non-receptor tyrosine kinase activity Reason: SRC is a non-receptor tyrosine kinase; tyrosine phosphorylation of protein substrates is its primary molecular activity.
GO:0005080 protein kinase C binding	ISO GO_REF:0000096	KEEP AS NON CORE	Summary: protein kinase C binding pathway context Reason: The annotation is compatible with SRC pathway biology but represents a context-specific process, location, or phenotype rather than the core kinase function.
GO:0005102 signaling receptor binding	ISO GO_REF:0000096	KEEP AS NON CORE	Summary: signaling receptor binding supports SRC recruitment or substrate recognition Reason: This interaction is mechanistically relevant to SRC signaling but is accessory to the core tyrosine kinase activity.
GO:0005158 insulin receptor binding	ISO GO_REF:0000096	KEEP AS NON CORE	Summary: insulin receptor binding pathway context Reason: The annotation is compatible with SRC pathway biology but represents a context-specific process, location, or phenotype rather than the core kinase function.
GO:0005737 cytoplasm	ISO GO_REF:0000096	ACCEPT	Summary: cytoplasm localization Reason: SRC is a soluble non-receptor kinase recruited to cytosolic, membrane, junctional, caveolar, and focal-adhesion signaling assemblies.
GO:0005901 caveola	ISO GO_REF:0000096	ACCEPT	Summary: caveola localization Reason: SRC is a soluble non-receptor kinase recruited to cytosolic, membrane, junctional, caveolar, and focal-adhesion signaling assemblies.
GO:0016020 membrane	ISO GO_REF:0000096	MARK AS OVER ANNOTATED	Summary: Generic membrane annotation Reason: The term is too broad or peripheral to capture SRC function when more specific tyrosine kinase and signaling annotations are available.
GO:0016579 protein deubiquitination	ISO GO_REF:0000119	KEEP AS NON CORE	Summary: protein deubiquitination pathway context Reason: The annotation is compatible with SRC pathway biology but represents a context-specific process, location, or phenotype rather than the core kinase function.
GO:0019899 enzyme binding	ISO GO_REF:0000096	MARK AS OVER ANNOTATED	Summary: Generic enzyme binding annotation Reason: The term is too broad or peripheral to capture SRC function when more specific tyrosine kinase and signaling annotations are available.
GO:0019901 protein kinase binding	ISO GO_REF:0000096	MARK AS OVER ANNOTATED	Summary: Generic protein kinase binding annotation Reason: The term is too broad or peripheral to capture SRC function when more specific tyrosine kinase and signaling annotations are available.
GO:0030424 axon	ISO GO_REF:0000096	KEEP AS NON CORE	Summary: axon pathway context Reason: The annotation is compatible with SRC pathway biology but represents a context-specific process, location, or phenotype rather than the core kinase function.
GO:0031669 cellular response to nutrient levels	ISO GO_REF:0000119	KEEP AS NON CORE	Summary: cellular response to nutrient levels pathway context Reason: This term captures a stimulus or pathway context in which SRC can act, not the core SRC molecular activity.
GO:0034138 toll-like receptor 3 signaling pathway	ISO GO_REF:0000119	KEEP AS NON CORE	Summary: toll-like receptor 3 signaling pathway pathway context Reason: The annotation is compatible with SRC pathway biology but represents a context-specific process, location, or phenotype rather than the core kinase function.
GO:0038202 TORC1 signaling	ISO GO_REF:0000119	KEEP AS NON CORE	Summary: TORC1 signaling pathway context Reason: The annotation is compatible with SRC pathway biology but represents a context-specific process, location, or phenotype rather than the core kinase function.
GO:0043025 neuronal cell body	ISO GO_REF:0000096	KEEP AS NON CORE	Summary: neuronal cell body pathway context Reason: The annotation is compatible with SRC pathway biology but represents a context-specific process, location, or phenotype rather than the core kinase function.
GO:0043161 proteasome-mediated ubiquitin-dependent protein catabolic process	ISO GO_REF:0000119	KEEP AS NON CORE	Summary: proteasome-mediated ubiquitin-dependent protein catabolic process pathway context Reason: The annotation is compatible with SRC pathway biology but represents a context-specific process, location, or phenotype rather than the core kinase function.
GO:0044294 dendritic growth cone	ISO GO_REF:0000096	KEEP AS NON CORE	Summary: dendritic growth cone pathway context Reason: The annotation is compatible with SRC pathway biology but represents a context-specific process, location, or phenotype rather than the core kinase function.
GO:0044877 protein-containing complex binding	ISO GO_REF:0000096	MARK AS OVER ANNOTATED	Summary: Generic protein-containing complex binding annotation Reason: The term is too broad or peripheral to capture SRC function when more specific tyrosine kinase and signaling annotations are available.
GO:0070374 positive regulation of ERK1 and ERK2 cascade	ISO GO_REF:0000096	KEEP AS NON CORE	Summary: positive regulation of ERK1 and ERK2 cascade pathway context Reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather than SRC core molecular activity.
GO:0071393 cellular response to progesterone stimulus	ISO GO_REF:0000096	KEEP AS NON CORE	Summary: cellular response to progesterone stimulus pathway context Reason: This term captures a stimulus or pathway context in which SRC can act, not the core SRC molecular activity.
GO:0071897 DNA biosynthetic process	ISO GO_REF:0000096	KEEP AS NON CORE	Summary: DNA biosynthetic process pathway context Reason: The annotation is compatible with SRC pathway biology but represents a context-specific process, location, or phenotype rather than the core kinase function.
GO:0090303 positive regulation of wound healing	ISO GO_REF:0000096	KEEP AS NON CORE	Summary: positive regulation of wound healing pathway context Reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather than SRC core molecular activity.
GO:0097060 synaptic membrane	ISO GO_REF:0000096	KEEP AS NON CORE	Summary: synaptic membrane pathway context Reason: The annotation is compatible with SRC pathway biology but represents a context-specific process, location, or phenotype rather than the core kinase function.
GO:0098978 glutamatergic synapse	ISO GO_REF:0000096	KEEP AS NON CORE	Summary: glutamatergic synapse pathway context Reason: The annotation is compatible with SRC pathway biology but represents a context-specific process, location, or phenotype rather than the core kinase function.
GO:0098984 neuron to neuron synapse	ISO GO_REF:0000096	KEEP AS NON CORE	Summary: neuron to neuron synapse pathway context Reason: The annotation is compatible with SRC pathway biology but represents a context-specific process, location, or phenotype rather than the core kinase function.
GO:0099073 mitochondrion-derived vesicle	ISO GO_REF:0000096	KEEP AS NON CORE	Summary: mitochondrion-derived vesicle pathway context Reason: The annotation is compatible with SRC pathway biology but represents a context-specific process, location, or phenotype rather than the core kinase function.
GO:0099091 postsynaptic specialization, intracellular component	ISO GO_REF:0000096	KEEP AS NON CORE	Summary: postsynaptic specialization, intracellular component pathway context Reason: The annotation is compatible with SRC pathway biology but represents a context-specific process, location, or phenotype rather than the core kinase function.
GO:1902533 positive regulation of intracellular signal transduction	ISO GO_REF:0000096	KEEP AS NON CORE	Summary: positive regulation of intracellular signal transduction pathway context Reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather than SRC core molecular activity.
GO:1902737 dendritic filopodium	ISO GO_REF:0000096	KEEP AS NON CORE	Summary: dendritic filopodium pathway context Reason: The annotation is compatible with SRC pathway biology but represents a context-specific process, location, or phenotype rather than the core kinase function.
GO:1904262 negative regulation of TORC1 signaling	ISO GO_REF:0000119	KEEP AS NON CORE	Summary: negative regulation of TORC1 signaling pathway context Reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather than SRC core molecular activity.
GO:1904707 positive regulation of vascular associated smooth muscle cell proliferation	ISO GO_REF:0000096	KEEP AS NON CORE	Summary: positive regulation of vascular associated smooth muscle cell proliferation pathway context Reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather than SRC core molecular activity.
GO:2000179 positive regulation of neural precursor cell proliferation	ISO GO_REF:0000096	KEEP AS NON CORE	Summary: positive regulation of neural precursor cell proliferation pathway context Reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather than SRC core molecular activity.
GO:2000256 positive regulation of male germ cell proliferation	ISO GO_REF:0000096	KEEP AS NON CORE	Summary: positive regulation of male germ cell proliferation pathway context Reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather than SRC core molecular activity.
GO:2000386 positive regulation of ovarian follicle development	ISO GO_REF:0000096	KEEP AS NON CORE	Summary: positive regulation of ovarian follicle development pathway context Reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather than SRC core molecular activity.
GO:0004713 protein tyrosine kinase activity	IDA PMID:18044967 Laminin-induced activation of Rac1 and JNKp46 is initiated b...	ACCEPT	Summary: Core SRC non-receptor tyrosine kinase activity Reason: SRC is a non-receptor tyrosine kinase; tyrosine phosphorylation of protein substrates is its primary molecular activity.
GO:0035022 positive regulation of Rac protein signal transduction	IMP PMID:18044967 Laminin-induced activation of Rac1 and JNKp46 is initiated b...	KEEP AS NON CORE	Summary: positive regulation of Rac protein signal transduction pathway context Reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather than SRC core molecular activity.
GO:0005515 protein binding	IPI PMID:25043309 Actin filament-associated protein 1 is required for cSrc act...	MARK AS OVER ANNOTATED	Summary: Generic protein binding Reason: Protein binding does not identify a specific SRC substrate-recognition mode or catalytic activity and is less informative than SH2/SH3/domain-specific terms.
GO:0005886 plasma membrane	IDA PMID:25043309 Actin filament-associated protein 1 is required for cSrc act...	ACCEPT	Summary: plasma membrane localization Reason: SRC is a soluble non-receptor kinase recruited to cytosolic, membrane, junctional, caveolar, and focal-adhesion signaling assemblies. Supporting Evidence: PMID:25043309 activating signals trigger the trafficking of cSrc to the plasma membrane and its concomitant activation
GO:0007595 lactation	IDA PMID:25043309 Actin filament-associated protein 1 is required for cSrc act...	KEEP AS NON CORE	Summary: lactation pathway context Reason: The annotation is compatible with SRC pathway biology but represents a context-specific process, location, or phenotype rather than the core kinase function.
GO:0035556 intracellular signal transduction	IMP PMID:12091389 Src-induced phosphorylation of caveolin-2 on tyrosine 19. Ph...	ACCEPT	Summary: SRC intracellular signaling function Reason: SRC acts near activated receptors and adhesion complexes to propagate tyrosine-phosphorylation-dependent intracellular signaling.
GO:0004713 protein tyrosine kinase activity	EXP PMID:22759635 A Src family kinase-Shp2 axis controls RUNX1 activity in meg...	ACCEPT	Summary: Core SRC non-receptor tyrosine kinase activity Reason: SRC is a non-receptor tyrosine kinase; tyrosine phosphorylation of protein substrates is its primary molecular activity.
GO:0004713 protein tyrosine kinase activity	TAS Reactome:R-MMU-3928622	ACCEPT	Summary: Core SRC non-receptor tyrosine kinase activity Reason: SRC is a non-receptor tyrosine kinase; tyrosine phosphorylation of protein substrates is its primary molecular activity.
GO:0004713 protein tyrosine kinase activity	TAS Reactome:R-MMU-418870	ACCEPT	Summary: Core SRC non-receptor tyrosine kinase activity Reason: SRC is a non-receptor tyrosine kinase; tyrosine phosphorylation of protein substrates is its primary molecular activity.
GO:0004713 protein tyrosine kinase activity	TAS Reactome:R-MMU-420382	ACCEPT	Summary: Core SRC non-receptor tyrosine kinase activity Reason: SRC is a non-receptor tyrosine kinase; tyrosine phosphorylation of protein substrates is its primary molecular activity.
GO:0004713 protein tyrosine kinase activity	TAS Reactome:R-MMU-9011252	ACCEPT	Summary: Core SRC non-receptor tyrosine kinase activity Reason: SRC is a non-receptor tyrosine kinase; tyrosine phosphorylation of protein substrates is its primary molecular activity.
GO:0004713 protein tyrosine kinase activity	TAS Reactome:R-MMU-9763891	ACCEPT	Summary: Core SRC non-receptor tyrosine kinase activity Reason: SRC is a non-receptor tyrosine kinase; tyrosine phosphorylation of protein substrates is its primary molecular activity.
GO:0004713 protein tyrosine kinase activity	TAS Reactome:R-NUL-9726861	ACCEPT	Summary: Core SRC non-receptor tyrosine kinase activity Reason: SRC is a non-receptor tyrosine kinase; tyrosine phosphorylation of protein substrates is its primary molecular activity.
GO:0005911 cell-cell junction	ISS GO_REF:0000024	ACCEPT	Summary: cell-cell junction localization Reason: SRC is a soluble non-receptor kinase recruited to cytosolic, membrane, junctional, caveolar, and focal-adhesion signaling assemblies.
GO:0038166 angiotensin-activated signaling pathway	IGI PMID:25017399 Caveolin-1 modulates cardiac gap junction homeostasis and ar...	KEEP AS NON CORE	Summary: angiotensin-activated signaling pathway pathway context Reason: The annotation is compatible with SRC pathway biology but represents a context-specific process, location, or phenotype rather than the core kinase function.
GO:0086091 regulation of heart rate by cardiac conduction	IGI PMID:25017399 Caveolin-1 modulates cardiac gap junction homeostasis and ar...	KEEP AS NON CORE	Summary: regulation of heart rate by cardiac conduction pathway context Reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather than SRC core molecular activity.
GO:0045821 positive regulation of glycolytic process	IDA PMID:35263589 Nociceptor-derived Reg3γ prevents endotoxic death by targeti...	KEEP AS NON CORE	Summary: positive regulation of glycolytic process pathway context Reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather than SRC core molecular activity.
GO:0004713 protein tyrosine kinase activity	IMP PMID:27505886 Tensin 3 is a new partner of Dock5 that controls osteoclast ...	ACCEPT	Summary: Core SRC non-receptor tyrosine kinase activity Reason: SRC is a non-receptor tyrosine kinase; tyrosine phosphorylation of protein substrates is its primary molecular activity.
GO:0018108 peptidyl-tyrosine phosphorylation	IMP PMID:27505886 Tensin 3 is a new partner of Dock5 that controls osteoclast ...	ACCEPT	Summary: Direct SRC tyrosine phosphorylation activity Reason: This term directly describes the catalytic output of SRC kinase activity on protein tyrosine residues.
GO:0045747 positive regulation of Notch signaling pathway	IDA PMID:25731159 A gp130-Src-YAP module links inflammation to epithelial rege...	KEEP AS NON CORE	Summary: positive regulation of Notch signaling pathway pathway context Reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather than SRC core molecular activity.
GO:0060576 intestinal epithelial cell development	IDA PMID:25731159 A gp130-Src-YAP module links inflammation to epithelial rege...	KEEP AS NON CORE	Summary: intestinal epithelial cell development pathway context Reason: The annotation is compatible with SRC pathway biology but represents a context-specific process, location, or phenotype rather than the core kinase function.
GO:0070102 interleukin-6-mediated signaling pathway	IDA PMID:25731159 A gp130-Src-YAP module links inflammation to epithelial rege...	KEEP AS NON CORE	Summary: interleukin-6-mediated signaling pathway pathway context Reason: The annotation is compatible with SRC pathway biology but represents a context-specific process, location, or phenotype rather than the core kinase function.
GO:0005515 protein binding	IPI PMID:11274221 Src and Pyk2 mediate G-protein-coupled receptor activation o...	MARK AS OVER ANNOTATED	Summary: Generic protein binding Reason: Protein binding does not identify a specific SRC substrate-recognition mode or catalytic activity and is less informative than SH2/SH3/domain-specific terms.
GO:0005743 mitochondrial inner membrane	IDA PMID:12615910 Regulation of cytochrome c oxidase activity by c-Src in oste...	KEEP AS NON CORE	Summary: mitochondrial inner membrane pathway context Reason: The annotation is compatible with SRC pathway biology but represents a context-specific process, location, or phenotype rather than the core kinase function.
GO:0030054 cell junction	ISS GO_REF:0000024	ACCEPT	Summary: cell junction localization Reason: SRC is a soluble non-receptor kinase recruited to cytosolic, membrane, junctional, caveolar, and focal-adhesion signaling assemblies.
GO:0005925 focal adhesion	IMP PMID:22801373 Protein tyrosine phosphatase α phosphotyrosyl-789 binds BCAR...	ACCEPT	Summary: focal adhesion localization Reason: SRC is a soluble non-receptor kinase recruited to cytosolic, membrane, junctional, caveolar, and focal-adhesion signaling assemblies.
GO:0004713 protein tyrosine kinase activity	IDA PMID:8910389 Bombesin, bradykinin, vasopressin, and phorbol esters rapidl...	ACCEPT	Summary: Core SRC non-receptor tyrosine kinase activity Reason: SRC is a non-receptor tyrosine kinase; tyrosine phosphorylation of protein substrates is its primary molecular activity.
GO:0005634 nucleus	IDA PMID:12615910 Regulation of cytochrome c oxidase activity by c-Src in oste...	KEEP AS NON CORE	Summary: nucleus pathway context Reason: The annotation is compatible with SRC pathway biology but represents a context-specific process, location, or phenotype rather than the core kinase function.
GO:0005739 mitochondrion	IDA PMID:12615910 Regulation of cytochrome c oxidase activity by c-Src in oste...	KEEP AS NON CORE	Summary: mitochondrion pathway context Reason: The annotation is compatible with SRC pathway biology but represents a context-specific process, location, or phenotype rather than the core kinase function.
GO:0005856 cytoskeleton	IDA PMID:12615910 Regulation of cytochrome c oxidase activity by c-Src in oste...	KEEP AS NON CORE	Summary: cytoskeleton pathway context Reason: The annotation is compatible with SRC pathway biology but represents a context-specific process, location, or phenotype rather than the core kinase function.
GO:0005886 plasma membrane	IDA PMID:12615910 Regulation of cytochrome c oxidase activity by c-Src in oste...	ACCEPT	Summary: plasma membrane localization Reason: SRC is a soluble non-receptor kinase recruited to cytosolic, membrane, junctional, caveolar, and focal-adhesion signaling assemblies. Supporting Evidence: PMID:12615910 c-Src as a plasma membrane-associated molecular effector of a variety of extracellular stimuli
GO:0016020 membrane	IMP PMID:22801373 Protein tyrosine phosphatase α phosphotyrosyl-789 binds BCAR...	MARK AS OVER ANNOTATED	Summary: Generic membrane annotation Reason: The term is too broad or peripheral to capture SRC function when more specific tyrosine kinase and signaling annotations are available.
GO:0005515 protein binding	IPI PMID:18691334 Palmitoylation-dependent endosomal localization of AATYK1A a...	MARK AS OVER ANNOTATED	Summary: Generic protein binding Reason: Protein binding does not identify a specific SRC substrate-recognition mode or catalytic activity and is less informative than SH2/SH3/domain-specific terms.
GO:0045453 bone resorption	IMP PMID:19448635 Impaired gastric acidification negatively affects calcium ho...	KEEP AS NON CORE	Summary: bone resorption pathway context Reason: The annotation is compatible with SRC pathway biology but represents a context-specific process, location, or phenotype rather than the core kinase function.
GO:0042476 odontogenesis	IMP PMID:23174213 Osteopetrosis, osteopetrorickets and hypophosphatemic ricket...	KEEP AS NON CORE	Summary: odontogenesis pathway context Reason: The annotation is compatible with SRC pathway biology but represents a context-specific process, location, or phenotype rather than the core kinase function.
GO:0070301 cellular response to hydrogen peroxide	IMP PMID:23651497 A signaling cascade mediated by ceramide, src and PDGFRβ coo...	KEEP AS NON CORE	Summary: cellular response to hydrogen peroxide pathway context Reason: This term captures a stimulus or pathway context in which SRC can act, not the core SRC molecular activity.
GO:2000588 positive regulation of platelet-derived growth factor receptor-beta signaling pathway	IMP PMID:23651497 A signaling cascade mediated by ceramide, src and PDGFRβ coo...	KEEP AS NON CORE	Summary: positive regulation of platelet-derived growth factor receptor-beta signaling pathway pathway context Reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather than SRC core molecular activity.
GO:0004672 protein kinase activity	IDA PMID:27994061 Focal Adhesion Kinase-mediated Phosphorylation of Beclin1 Pr...	MARK AS OVER ANNOTATED	Summary: protein kinase activity is less specific than SRC tyrosine kinase activity Reason: The annotation is directionally correct but over-broad because more specific SRC tyrosine kinase terms are present.
GO:0006468 protein phosphorylation	IDA PMID:27994061 Focal Adhesion Kinase-mediated Phosphorylation of Beclin1 Pr...	UNDECIDED	Summary: PMID:27994061 supports FAK phosphorylation of Beclin1, but the cached abstract does not directly support Src-mediated protein phosphorylation. Reason: Full text or a direct Src-specific passage would be needed before accepting this evidence-specific annotation.
GO:0005886 plasma membrane	TAS Reactome:R-MMU-9011252	ACCEPT	Summary: plasma membrane localization Reason: SRC is a soluble non-receptor kinase recruited to cytosolic, membrane, junctional, caveolar, and focal-adhesion signaling assemblies.
GO:0005886 plasma membrane	TAS Reactome:R-NUL-9726861	ACCEPT	Summary: plasma membrane localization Reason: SRC is a soluble non-receptor kinase recruited to cytosolic, membrane, junctional, caveolar, and focal-adhesion signaling assemblies.
GO:0005829 cytosol	TAS Reactome:R-NUL-9700655	ACCEPT	Summary: cytosol localization Reason: SRC is a soluble non-receptor kinase recruited to cytosolic, membrane, junctional, caveolar, and focal-adhesion signaling assemblies.
GO:0005737 cytoplasm	IDA PMID:14699011 Regulation of connexin43 protein complexes by intracellular ...	ACCEPT	Summary: cytoplasm localization Reason: SRC is a soluble non-receptor kinase recruited to cytosolic, membrane, junctional, caveolar, and focal-adhesion signaling assemblies.
GO:0071253 connexin binding	IPI PMID:14699011 Regulation of connexin43 protein complexes by intracellular ...	KEEP AS NON CORE	Summary: connexin binding pathway context Reason: The annotation is compatible with SRC pathway biology but represents a context-specific process, location, or phenotype rather than the core kinase function.
GO:0005654 nucleoplasm	TAS Reactome:R-MMU-8937703	KEEP AS NON CORE	Summary: nucleoplasm pathway context Reason: The annotation is compatible with SRC pathway biology but represents a context-specific process, location, or phenotype rather than the core kinase function.
GO:0005654 nucleoplasm	TAS Reactome:R-MMU-8937738	KEEP AS NON CORE	Summary: nucleoplasm pathway context Reason: The annotation is compatible with SRC pathway biology but represents a context-specific process, location, or phenotype rather than the core kinase function.
GO:0002102 podosome	IDA PMID:17893324 Src-dependent phosphorylation of ASAP1 regulates podosomes.	KEEP AS NON CORE	Summary: podosome pathway context Reason: The annotation is compatible with SRC pathway biology but represents a context-specific process, location, or phenotype rather than the core kinase function.
GO:0010954 positive regulation of protein processing	IGI PMID:17344476 The transcriptional repressor Glis2 is a novel binding partn...	KEEP AS NON CORE	Summary: positive regulation of protein processing pathway context Reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather than SRC core molecular activity.
GO:0050731 positive regulation of peptidyl-tyrosine phosphorylation	IGI PMID:17344476 The transcriptional repressor Glis2 is a novel binding partn...	KEEP AS NON CORE	Summary: positive regulation of peptidyl-tyrosine phosphorylation pathway context Reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather than SRC core molecular activity.
GO:1900182 positive regulation of protein localization to nucleus	IGI PMID:17344476 The transcriptional repressor Glis2 is a novel binding partn...	KEEP AS NON CORE	Summary: positive regulation of protein localization to nucleus pathway context Reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather than SRC core molecular activity.
GO:0005515 protein binding	IPI PMID:20847314 CXCR4-mediated bone marrow progenitor cell maintenance and m...	MARK AS OVER ANNOTATED	Summary: Generic protein binding Reason: Protein binding does not identify a specific SRC substrate-recognition mode or catalytic activity and is less informative than SH2/SH3/domain-specific terms.
GO:0071803 positive regulation of podosome assembly	IDA PMID:24127566 Phosphorylation of moesin by Jun N-terminal kinase is import...	KEEP AS NON CORE	Summary: positive regulation of podosome assembly pathway context Reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather than SRC core molecular activity.
GO:0004672 protein kinase activity	IDA PMID:24190966 A reactive oxygen species-mediated, self-perpetuating loop p...	MARK AS OVER ANNOTATED	Summary: protein kinase activity is less specific than SRC tyrosine kinase activity Reason: The annotation is directionally correct but over-broad because more specific SRC tyrosine kinase terms are present.
GO:0005515 protein binding	IPI PMID:24190966 A reactive oxygen species-mediated, self-perpetuating loop p...	MARK AS OVER ANNOTATED	Summary: Generic protein binding Reason: Protein binding does not identify a specific SRC substrate-recognition mode or catalytic activity and is less informative than SH2/SH3/domain-specific terms.
GO:0034614 cellular response to reactive oxygen species	IDA PMID:24190966 A reactive oxygen species-mediated, self-perpetuating loop p...	KEEP AS NON CORE	Summary: cellular response to reactive oxygen species pathway context Reason: This term captures a stimulus or pathway context in which SRC can act, not the core SRC molecular activity.
GO:0005634 nucleus	IDA PMID:24841674 Mechanical loading in osteocytes induces formation of a Src/...	KEEP AS NON CORE	Summary: nucleus pathway context Reason: The annotation is compatible with SRC pathway biology but represents a context-specific process, location, or phenotype rather than the core kinase function.
GO:0048471 perinuclear region of cytoplasm	IDA PMID:24841674 Mechanical loading in osteocytes induces formation of a Src/...	KEEP AS NON CORE	Summary: perinuclear region of cytoplasm pathway context Reason: The annotation is compatible with SRC pathway biology but represents a context-specific process, location, or phenotype rather than the core kinase function.
GO:0071498 cellular response to fluid shear stress	IMP PMID:24841674 Mechanical loading in osteocytes induces formation of a Src/...	KEEP AS NON CORE	Summary: cellular response to fluid shear stress pathway context Reason: This term captures a stimulus or pathway context in which SRC can act, not the core SRC molecular activity.
GO:0004713 protein tyrosine kinase activity	IMP PMID:12091389 Src-induced phosphorylation of caveolin-2 on tyrosine 19. Ph...	ACCEPT	Summary: Core SRC non-receptor tyrosine kinase activity Reason: SRC is a non-receptor tyrosine kinase; tyrosine phosphorylation of protein substrates is its primary molecular activity.
GO:0005901 caveola	IDA PMID:12091389 Src-induced phosphorylation of caveolin-2 on tyrosine 19. Ph...	ACCEPT	Summary: caveola localization Reason: SRC is a soluble non-receptor kinase recruited to cytosolic, membrane, junctional, caveolar, and focal-adhesion signaling assemblies.
GO:0097110 scaffold protein binding	IPI PMID:12091389 Src-induced phosphorylation of caveolin-2 on tyrosine 19. Ph...	KEEP AS NON CORE	Summary: scaffold protein binding supports SRC recruitment or substrate recognition Reason: This interaction is mechanistically relevant to SRC signaling but is accessory to the core tyrosine kinase activity.
GO:0031648 protein destabilization	IMP PMID:25259869 Genetic disruption of the sh3pxd2a gene reveals an essential...	KEEP AS NON CORE	Summary: protein destabilization pathway context Reason: The annotation is compatible with SRC pathway biology but represents a context-specific process, location, or phenotype rather than the core kinase function.
GO:0071375 cellular response to peptide hormone stimulus	IGI PMID:25017399 Caveolin-1 modulates cardiac gap junction homeostasis and ar...	KEEP AS NON CORE	Summary: cellular response to peptide hormone stimulus pathway context Reason: This term captures a stimulus or pathway context in which SRC can act, not the core SRC molecular activity.
GO:0097110 scaffold protein binding	IPI PMID:25017399 Caveolin-1 modulates cardiac gap junction homeostasis and ar...	KEEP AS NON CORE	Summary: scaffold protein binding supports SRC recruitment or substrate recognition Reason: This interaction is mechanistically relevant to SRC signaling but is accessory to the core tyrosine kinase activity.
GO:0071560 cellular response to transforming growth factor beta stimulus	IGI PMID:21829547 Redox-induced Src kinase and caveolin-1 signaling in TGF-β1-...	KEEP AS NON CORE	Summary: cellular response to transforming growth factor beta stimulus pathway context Reason: This term captures a stimulus or pathway context in which SRC can act, not the core SRC molecular activity.
GO:0005884 actin filament	IDA PMID:17623777 The membrane targeting and spatial activation of Src, Yes an...	KEEP AS NON CORE	Summary: actin filament pathway context Reason: The annotation is compatible with SRC pathway biology but represents a context-specific process, location, or phenotype rather than the core kinase function.
GO:0032587 ruffle membrane	IDA PMID:17623777 The membrane targeting and spatial activation of Src, Yes an...	KEEP AS NON CORE	Summary: ruffle membrane pathway context Reason: The annotation is compatible with SRC pathway biology but represents a context-specific process, location, or phenotype rather than the core kinase function.
GO:0036120 cellular response to platelet-derived growth factor stimulus	IDA PMID:17623777 The membrane targeting and spatial activation of Src, Yes an...	KEEP AS NON CORE	Summary: cellular response to platelet-derived growth factor stimulus pathway context Reason: This term captures a stimulus or pathway context in which SRC can act, not the core SRC molecular activity.
GO:0048471 perinuclear region of cytoplasm	IDA PMID:17623777 The membrane targeting and spatial activation of Src, Yes an...	KEEP AS NON CORE	Summary: perinuclear region of cytoplasm pathway context Reason: The annotation is compatible with SRC pathway biology but represents a context-specific process, location, or phenotype rather than the core kinase function.
GO:0004672 protein kinase activity	IMP PMID:23100514 Abl family kinases regulate FcγR-mediated phagocytosis in mu...	MARK AS OVER ANNOTATED	Summary: protein kinase activity is less specific than SRC tyrosine kinase activity Reason: The annotation is directionally correct but over-broad because more specific SRC tyrosine kinase terms are present.
GO:0034446 substrate adhesion-dependent cell spreading	IDA PMID:11864995 Modulation of the F-actin cytoskeleton by c-Abl tyrosine kin...	KEEP AS NON CORE	Summary: substrate adhesion-dependent cell spreading pathway context Reason: The annotation is compatible with SRC pathway biology but represents a context-specific process, location, or phenotype rather than the core kinase function.
GO:0060491 regulation of cell projection assembly	IGI PMID:11864995 Modulation of the F-actin cytoskeleton by c-Abl tyrosine kin...	KEEP AS NON CORE	Summary: regulation of cell projection assembly pathway context Reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather than SRC core molecular activity.
GO:0035556 intracellular signal transduction	IGI PMID:23526378 Targeted disruption of leucine-rich repeat kinase 1 but not ...	KEEP AS NON CORE	Summary: SRC intracellular signaling function Reason: This Lrrk1 osteoclast study supports Src pathway involvement, but intracellular signal transduction is broad relative to SRC tyrosine kinase activity. Supporting Evidence: PMID:23526378 Lrrk1 may function at least in part through modulating the c-Src signaling pathway.
GO:0036035 osteoclast development	IGI PMID:23526378 Targeted disruption of leucine-rich repeat kinase 1 but not ...	KEEP AS NON CORE	Summary: osteoclast development pathway context Reason: The annotation is compatible with SRC pathway biology but represents a context-specific process, location, or phenotype rather than the core kinase function.
GO:0018108 peptidyl-tyrosine phosphorylation	ISO PMID:22732588 Interchangeability of Themis1 and Themis2 in thymocyte devel...	UNDECIDED	Summary: PMID:22732588 supports tyrosine phosphorylation of Themis proteins after TCR engagement, but the cached text does not directly support Src as the kinase for this annotation. Reason: Full text or a direct Src-family kinase passage would be needed before accepting this ISO transfer as Src peptidyl-tyrosine phosphorylation evidence.
GO:0005515 protein binding	IPI PMID:11826110 Delphilin: a novel PDZ and formin homology domain-containing...	MARK AS OVER ANNOTATED	Summary: Generic protein binding Reason: Protein binding does not identify a specific SRC substrate-recognition mode or catalytic activity and is less informative than SH2/SH3/domain-specific terms.
GO:0005515 protein binding	IPI PMID:8861907 Mena, a relative of VASP and Drosophila Enabled, is implicat...	MARK AS OVER ANNOTATED	Summary: Generic protein binding Reason: Protein binding does not identify a specific SRC substrate-recognition mode or catalytic activity and is less informative than SH2/SH3/domain-specific terms.
GO:0005515 protein binding	IPI PMID:21525037 The guanine nucleotide exchange factor Arhgef5 plays crucial...	MARK AS OVER ANNOTATED	Summary: Generic protein binding Reason: Protein binding does not identify a specific SRC substrate-recognition mode or catalytic activity and is less informative than SH2/SH3/domain-specific terms.
GO:0005886 plasma membrane	IDA PMID:21525037 The guanine nucleotide exchange factor Arhgef5 plays crucial...	ACCEPT	Summary: plasma membrane localization Reason: SRC is a soluble non-receptor kinase recruited to cytosolic, membrane, junctional, caveolar, and focal-adhesion signaling assemblies.
GO:0071803 positive regulation of podosome assembly	IDA PMID:21525037 The guanine nucleotide exchange factor Arhgef5 plays crucial...	KEEP AS NON CORE	Summary: positive regulation of podosome assembly pathway context Reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather than SRC core molecular activity.
GO:0071803 positive regulation of podosome assembly	IGI PMID:21525037 The guanine nucleotide exchange factor Arhgef5 plays crucial...	KEEP AS NON CORE	Summary: positive regulation of podosome assembly pathway context Reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather than SRC core molecular activity.
GO:0005515 protein binding	IPI PMID:14600260 A caveolin-3 mutant that causes limb girdle muscular dystrop...	MARK AS OVER ANNOTATED	Summary: Generic protein binding Reason: Protein binding does not identify a specific SRC substrate-recognition mode or catalytic activity and is less informative than SH2/SH3/domain-specific terms.
GO:0005515 protein binding	IPI PMID:17000758 Ccpg1, a novel scaffold protein that regulates the activity ...	MARK AS OVER ANNOTATED	Summary: Generic protein binding Reason: Protein binding does not identify a specific SRC substrate-recognition mode or catalytic activity and is less informative than SH2/SH3/domain-specific terms.
GO:0046875 ephrin receptor binding	IPI PMID:12925710 EphB1 recruits c-Src and p52Shc to activate MAPK/ERK and pro...	KEEP AS NON CORE	Summary: ephrin receptor binding supports SRC recruitment or substrate recognition Reason: This interaction is mechanistically relevant to SRC signaling but is accessory to the core tyrosine kinase activity.
GO:0005515 protein binding	IPI PMID:14739300 Src kinase activity is essential for osteoclast function.	MARK AS OVER ANNOTATED	Summary: Generic protein binding Reason: Protein binding does not identify a specific SRC substrate-recognition mode or catalytic activity and is less informative than SH2/SH3/domain-specific terms.
GO:0020037 heme binding	ISS GO_REF:0000024	MARK AS OVER ANNOTATED	Summary: Generic heme binding annotation Reason: The term is too broad or peripheral to capture SRC function when more specific tyrosine kinase and signaling annotations are available.
GO:0045453 bone resorption	IMP PMID:14739300 Src kinase activity is essential for osteoclast function.	KEEP AS NON CORE	Summary: bone resorption pathway context Reason: The annotation is compatible with SRC pathway biology but represents a context-specific process, location, or phenotype rather than the core kinase function.
GO:0004672 protein kinase activity	IDA PMID:19920076 Dishevelled-2 docks and activates Src in a Wnt-dependent man...	MARK AS OVER ANNOTATED	Summary: protein kinase activity is less specific than SRC tyrosine kinase activity Reason: The annotation is directionally correct but over-broad because more specific SRC tyrosine kinase terms are present.
GO:0005515 protein binding	IPI PMID:19920076 Dishevelled-2 docks and activates Src in a Wnt-dependent man...	MARK AS OVER ANNOTATED	Summary: Generic protein binding Reason: Protein binding does not identify a specific SRC substrate-recognition mode or catalytic activity and is less informative than SH2/SH3/domain-specific terms.
GO:0090263 positive regulation of canonical Wnt signaling pathway	IGI PMID:19920076 Dishevelled-2 docks and activates Src in a Wnt-dependent man...	KEEP AS NON CORE	Summary: positive regulation of canonical Wnt signaling pathway pathway context Reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather than SRC core molecular activity.
GO:0005829 cytosol	TAS Reactome:R-MMU-1433273	ACCEPT	Summary: cytosol localization Reason: SRC is a soluble non-receptor kinase recruited to cytosolic, membrane, junctional, caveolar, and focal-adhesion signaling assemblies.
GO:0005829 cytosol	TAS Reactome:R-MMU-3928622	ACCEPT	Summary: cytosol localization Reason: SRC is a soluble non-receptor kinase recruited to cytosolic, membrane, junctional, caveolar, and focal-adhesion signaling assemblies.
GO:0005829 cytosol	TAS Reactome:R-MMU-418870	ACCEPT	Summary: cytosol localization Reason: SRC is a soluble non-receptor kinase recruited to cytosolic, membrane, junctional, caveolar, and focal-adhesion signaling assemblies.
GO:0005829 cytosol	TAS Reactome:R-MMU-420382	ACCEPT	Summary: cytosol localization Reason: SRC is a soluble non-receptor kinase recruited to cytosolic, membrane, junctional, caveolar, and focal-adhesion signaling assemblies.
GO:0005829 cytosol	TAS Reactome:R-MMU-5218834	ACCEPT	Summary: cytosol localization Reason: SRC is a soluble non-receptor kinase recruited to cytosolic, membrane, junctional, caveolar, and focal-adhesion signaling assemblies.
GO:0005829 cytosol	TAS Reactome:R-MMU-8964229	ACCEPT	Summary: cytosol localization Reason: SRC is a soluble non-receptor kinase recruited to cytosolic, membrane, junctional, caveolar, and focal-adhesion signaling assemblies.
GO:0005829 cytosol	TAS Reactome:R-MMU-9680646	ACCEPT	Summary: cytosol localization Reason: SRC is a soluble non-receptor kinase recruited to cytosolic, membrane, junctional, caveolar, and focal-adhesion signaling assemblies.
GO:0005829 cytosol	TAS Reactome:R-MMU-9680706	ACCEPT	Summary: cytosol localization Reason: SRC is a soluble non-receptor kinase recruited to cytosolic, membrane, junctional, caveolar, and focal-adhesion signaling assemblies.
GO:0005829 cytosol	TAS Reactome:R-MMU-9682158	ACCEPT	Summary: cytosol localization Reason: SRC is a soluble non-receptor kinase recruited to cytosolic, membrane, junctional, caveolar, and focal-adhesion signaling assemblies.
GO:0005829 cytosol	TAS Reactome:R-MMU-9682182	ACCEPT	Summary: cytosol localization Reason: SRC is a soluble non-receptor kinase recruited to cytosolic, membrane, junctional, caveolar, and focal-adhesion signaling assemblies.
GO:0005829 cytosol	TAS Reactome:R-MMU-9682572	ACCEPT	Summary: cytosol localization Reason: SRC is a soluble non-receptor kinase recruited to cytosolic, membrane, junctional, caveolar, and focal-adhesion signaling assemblies.
GO:0005829 cytosol	TAS Reactome:R-MMU-9763891	ACCEPT	Summary: cytosol localization Reason: SRC is a soluble non-receptor kinase recruited to cytosolic, membrane, junctional, caveolar, and focal-adhesion signaling assemblies.
GO:0005829 cytosol	TAS Reactome:R-MMU-9763892	ACCEPT	Summary: cytosol localization Reason: SRC is a soluble non-receptor kinase recruited to cytosolic, membrane, junctional, caveolar, and focal-adhesion signaling assemblies.
GO:0005829 cytosol	TAS Reactome:R-MMU-9763903	ACCEPT	Summary: cytosol localization Reason: SRC is a soluble non-receptor kinase recruited to cytosolic, membrane, junctional, caveolar, and focal-adhesion signaling assemblies.
GO:0005829 cytosol	TAS Reactome:R-MMU-9764150	ACCEPT	Summary: cytosol localization Reason: SRC is a soluble non-receptor kinase recruited to cytosolic, membrane, junctional, caveolar, and focal-adhesion signaling assemblies.
GO:0005829 cytosol	TAS Reactome:R-MMU-9817994	ACCEPT	Summary: cytosol localization Reason: SRC is a soluble non-receptor kinase recruited to cytosolic, membrane, junctional, caveolar, and focal-adhesion signaling assemblies.
GO:0005829 cytosol	TAS Reactome:R-MMU-9818009	ACCEPT	Summary: cytosol localization Reason: SRC is a soluble non-receptor kinase recruited to cytosolic, membrane, junctional, caveolar, and focal-adhesion signaling assemblies.
GO:0005829 cytosol	TAS Reactome:R-NUL-9700661	ACCEPT	Summary: cytosol localization Reason: SRC is a soluble non-receptor kinase recruited to cytosolic, membrane, junctional, caveolar, and focal-adhesion signaling assemblies.
GO:0070374 positive regulation of ERK1 and ERK2 cascade	IMP PMID:17045821 CD44 is the signaling component of the macrophage migration ...	KEEP AS NON CORE	Summary: positive regulation of ERK1 and ERK2 cascade pathway context Reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather than SRC core molecular activity.
GO:0033146 regulation of intracellular estrogen receptor signaling pathway	IMP PMID:16007215 c-Src-null mice exhibit defects in normal mammary gland deve...	KEEP AS NON CORE	Summary: regulation of intracellular estrogen receptor signaling pathway pathway context Reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather than SRC core molecular activity.
GO:0048477 oogenesis	IMP PMID:16007215 c-Src-null mice exhibit defects in normal mammary gland deve...	KEEP AS NON CORE	Summary: oogenesis pathway context Reason: The annotation is compatible with SRC pathway biology but represents a context-specific process, location, or phenotype rather than the core kinase function.
GO:0060065 uterus development	IMP PMID:16007215 c-Src-null mice exhibit defects in normal mammary gland deve...	KEEP AS NON CORE	Summary: uterus development pathway context Reason: The annotation is compatible with SRC pathway biology but represents a context-specific process, location, or phenotype rather than the core kinase function.
GO:0060444 branching involved in mammary gland duct morphogenesis	IMP PMID:16007215 c-Src-null mice exhibit defects in normal mammary gland deve...	KEEP AS NON CORE	Summary: branching involved in mammary gland duct morphogenesis pathway context Reason: The annotation is compatible with SRC pathway biology but represents a context-specific process, location, or phenotype rather than the core kinase function.
GO:0019904 protein domain specific binding	IPI PMID:8438166 Identification of a ten-amino acid proline-rich SH3 binding ...	KEEP AS NON CORE	Summary: protein domain specific binding supports SRC recruitment or substrate recognition Reason: This interaction is mechanistically relevant to SRC signaling but is accessory to the core tyrosine kinase activity.
GO:0006468 protein phosphorylation	IDA PMID:15937334 A role for mitogen-activated protein kinase(Erk1/2) activati...	UNDECIDED	Summary: PMID:15937334 supports chronological activation of Src-family tyrosine kinases in P2X7R-mediated thymocyte death, but does not identify Src specifically in the cached abstract. Reason: Full text would be needed to confirm this as a direct Src protein-phosphorylation annotation.
GO:0016477 cell migration	IMP PMID:15292044 Alterations in cell-adhesive and migratory properties of pro...	KEEP AS NON CORE	Summary: cell migration pathway context Reason: The annotation is compatible with SRC pathway biology but represents a context-specific process, location, or phenotype rather than the core kinase function.
GO:0018108 peptidyl-tyrosine phosphorylation	IDA PMID:12526740 Reelin activates SRC family tyrosine kinases in neurons.	ACCEPT	Summary: PMID:12526740 shows Reelin-dependent activation of Src-family non-receptor tyrosine kinases in neurons, with Dab1 tyrosine phosphorylation by SFKs. Reason: The current term is already the tyrosine-specific phosphorylation process supported by the Reelin/Src-family kinase study, so no replacement is needed. Supporting Evidence: PMID:12526740 Dab1 is tyrosine phosphorylated by SFKs
GO:0030900 forebrain development	IGI PMID:16162939 Absence of Fyn and Src causes a reeler-like phenotype.	KEEP AS NON CORE	Summary: forebrain development pathway context Reason: The annotation is compatible with SRC pathway biology but represents a context-specific process, location, or phenotype rather than the core kinase function.
GO:0016301 kinase activity	IMP PMID:12808090 Gab1 is an integrator of cell death versus cell survival sig...	MARK AS OVER ANNOTATED	Summary: kinase activity is less specific than SRC tyrosine kinase activity Reason: The annotation is directionally correct but over-broad because more specific SRC tyrosine kinase terms are present.
GO:0004713 protein tyrosine kinase activity	IMP PMID:14636584 Phosphorylation-dependent paxillin-ERK association mediates ...	ACCEPT	Summary: Core SRC non-receptor tyrosine kinase activity Reason: SRC is a non-receptor tyrosine kinase; tyrosine phosphorylation of protein substrates is its primary molecular activity.
GO:0004672 protein kinase activity	IDA PMID:11274221 Src and Pyk2 mediate G-protein-coupled receptor activation o...	MARK AS OVER ANNOTATED	Summary: protein kinase activity is less specific than SRC tyrosine kinase activity Reason: The annotation is directionally correct but over-broad because more specific SRC tyrosine kinase terms are present.
GO:0006468 protein phosphorylation	IDA PMID:11274221 Src and Pyk2 mediate G-protein-coupled receptor activation o...	ACCEPT	Summary: Direct SRC tyrosine phosphorylation activity Reason: This term directly describes the catalytic output of SRC kinase activity on protein tyrosine residues.

Core Functions

SRC phosphorylates protein tyrosine residues as a regulated non-receptor kinase assembled with receptor, adaptor, and cytoskeletal signaling complexes through SH2 and SH3 domain interactions.

Molecular Function:

non-membrane spanning protein tyrosine kinase activity

Directly Involved In:

peptidyl-tyrosine phosphorylation intracellular signal transduction

Cellular Locations:

cytosol plasma membrane focal adhesion

Supporting Evidence:

file:mouse/Src/Src-notes.md
SRC is a non-receptor protein tyrosine kinase with SH3-SH2-kinase architecture.
file:mouse/Src/Src-deep-research-falcon.md
Falcon synthesis supports Src as a regulated non-receptor tyrosine kinase that phosphorylates protein substrates in receptor, adhesion, and cytoskeletal signaling complexes.

References

GO_REF:0000002

Gene Ontology annotation through association of InterPro records with GO terms

GO_REF:0000024

Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity

GO_REF:0000033

Annotation inferences using phylogenetic trees

GO_REF:0000044

Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt

GO_REF:0000096

Automated transfer of experimentally-verified manual GO annotation data to mouse-rat orthologs

GO_REF:0000107

Automatic transfer of experimentally verified manual GO annotation data to orthologs using Ensembl Compara

GO_REF:0000117

Electronic Gene Ontology annotations created by ARBA machine learning models

GO_REF:0000119

Automated transfer of experimentally-verified manual GO annotation data to mouse-human orthologs

GO_REF:0000120

Combined Automated Annotation using Multiple IEA Methods

PMID:10220404

Identification of profilin and src homology 3 domains as binding partners for Drosophila enabled.

PMID:11274221

Src and Pyk2 mediate G-protein-coupled receptor activation of epidermal growth factor receptor (EGFR) but are not required for coupling to the mitogen-activated protein (MAP) kinase signaling cascade.

PMID:11826110

Delphilin: a novel PDZ and formin homology domain-containing protein that synaptically colocalizes and interacts with glutamate receptor delta 2 subunit.

PMID:11864995

Modulation of the F-actin cytoskeleton by c-Abl tyrosine kinase in cell spreading and neurite extension.

PMID:12091389

Src-induced phosphorylation of caveolin-2 on tyrosine 19. Phospho-caveolin-2 (Tyr(P)19) is localized near focal adhesions, remains associated with lipid rafts/caveolae, but no longer forms a high molecular mass hetero-oligomer with caveolin-1.

PMID:12526740

Reelin activates SRC family tyrosine kinases in neurons.

Reelin activates Src-family non-receptor tyrosine kinases in neurons and supports Dab1 tyrosine phosphorylation as Src-family kinase output

"We show that Reelin activates members of the Src family of non-receptor tyrosine kinases (SFKs)"

PMID:12615910

Regulation of cytochrome c oxidase activity by c-Src in osteoclasts.

PMID:12808090

Gab1 is an integrator of cell death versus cell survival signals in oxidative stress.

PMID:12925710

EphB1 recruits c-Src and p52Shc to activate MAPK/ERK and promote chemotaxis.

PMID:14600260

A caveolin-3 mutant that causes limb girdle muscular dystrophy type 1C disrupts Src localization and activity and induces apoptosis in skeletal myotubes.

PMID:14636584

Phosphorylation-dependent paxillin-ERK association mediates hepatocyte growth factor-stimulated epithelial morphogenesis.

PMID:14699011

Regulation of connexin43 protein complexes by intracellular acidification.

PMID:14739300

Src kinase activity is essential for osteoclast function.

PMID:15292044

Alterations in cell-adhesive and migratory properties of proximal tubule and collecting duct cells from bcl-2 -/- mice.

PMID:15937334

A role for mitogen-activated protein kinase(Erk1/2) activation and non-selective pore formation in P2X7 receptor-mediated thymocyte death.

PMID:16007215

c-Src-null mice exhibit defects in normal mammary gland development and ERalpha signaling.

PMID:16162939

Absence of Fyn and Src causes a reeler-like phenotype.

PMID:16267818

A proteomics strategy for the enrichment of receptor-associated complexes.

PMID:16298995

EphB receptors regulate dendritic spine morphogenesis through the recruitment/phosphorylation of focal adhesion kinase and RhoA activation.

PMID:16407161

Role of Src in C3 transient receptor potential channel function and evidence for a heterogeneous makeup of receptor- and store-operated Ca2+ entry channels.

PMID:16990796

PSD-95 is a negative regulator of the tyrosine kinase Src in the NMDA receptor complex.

PMID:17000758

Ccpg1, a novel scaffold protein that regulates the activity of the Rho guanine nucleotide exchange factor Dbs.

PMID:17045821

CD44 is the signaling component of the macrophage migration inhibitory factor-CD74 receptor complex.

PMID:17344476

The transcriptional repressor Glis2 is a novel binding partner for p120 catenin.

PMID:17623777

The membrane targeting and spatial activation of Src, Yes and Fyn is influenced by palmitoylation and distinct RhoB/RhoD endosome requirements.

PMID:17893324

Src-dependent phosphorylation of ASAP1 regulates podosomes.

PMID:18044967

Laminin-induced activation of Rac1 and JNKp46 is initiated by Src family kinases and mimics the effects of skeletal muscle contraction.

PMID:18289379

Dramatic inhibition of osteoclast sealing ring formation and bone resorption in vitro by a WASP-peptide containing pTyr294 amino acid.

PMID:18560762

Mobility of TOAC spin-labelled peptides binding to the Src SH3 domain studied by paramagnetic NMR.

PMID:18691334

Palmitoylation-dependent endosomal localization of AATYK1A and its interaction with Src.

PMID:19122674

Deficiency of a beta-arrestin-2 signal complex contributes to insulin resistance.

PMID:19448635

Impaired gastric acidification negatively affects calcium homeostasis and bone mass.

PMID:19920076

Dishevelled-2 docks and activates Src in a Wnt-dependent manner.

PMID:20847314

CXCR4-mediated bone marrow progenitor cell maintenance and mobilization are modulated by c-kit activity.

PMID:21199370

Roles of the SH2 and SH3 domains in the regulation of neuronal Src kinase functions.

PMID:21266176

Linker length dependent binding of a focal adhesion kinase derived peptide to the Src SH3-SH2 domains.

PMID:21282606

Nε-lysine acetylation determines dissociation from GAP junctions and lateralization of connexin 43 in normal and dystrophic heart.

PMID:21525037

The guanine nucleotide exchange factor Arhgef5 plays crucial roles in Src-induced podosome formation.

PMID:21829547

Redox-induced Src kinase and caveolin-1 signaling in TGF-β1-initiated SMAD2/3 activation and PAI-1 expression.

PMID:22439932

NKX2-1/TITF1/TTF-1-Induced ROR1 is required to sustain EGFR survival signaling in lung adenocarcinoma.

PMID:22732588

Interchangeability of Themis1 and Themis2 in thymocyte development reveals two related proteins with conserved molecular function.

PMID:22759635

A Src family kinase-Shp2 axis controls RUNX1 activity in megakaryocyte and T-lymphocyte differentiation.

PMID:22801373

Protein tyrosine phosphatase α phosphotyrosyl-789 binds BCAR3 to position Cas for activation at integrin-mediated focal adhesions.

PMID:23100514

Abl family kinases regulate FcγR-mediated phagocytosis in murine macrophages.

PMID:23174213

Osteopetrosis, osteopetrorickets and hypophosphatemic rickets differentially affect dentin and enamel mineralization.

PMID:23526378

Targeted disruption of leucine-rich repeat kinase 1 but not leucine-rich repeat kinase 2 in mice causes severe osteopetrosis.

PMID:23651497

A signaling cascade mediated by ceramide, src and PDGFRβ coordinates the activation of the redox-sensitive neutral sphingomyelinase-2 and sphingosine kinase-1.

PMID:24127566

Phosphorylation of moesin by Jun N-terminal kinase is important for podosome rosette formation in Src-transformed fibroblasts.

PMID:24190966

A reactive oxygen species-mediated, self-perpetuating loop persistently activates platelet-derived growth factor receptor α.

PMID:24841674

Mechanical loading in osteocytes induces formation of a Src/Pyk2/MBD2 complex that suppresses anabolic gene expression.

PMID:25017399

Caveolin-1 modulates cardiac gap junction homeostasis and arrhythmogenecity by regulating cSrc tyrosine kinase.

PMID:25043309

Actin filament-associated protein 1 is required for cSrc activity and secretory activation in the lactating mammary gland.

PMID:25259869

Genetic disruption of the sh3pxd2a gene reveals an essential role in mouse development and the existence of a novel isoform of tks5.

PMID:25731159

A gp130-Src-YAP module links inflammation to epithelial regeneration.

PMID:27505886

Tensin 3 is a new partner of Dock5 that controls osteoclast podosome organization and activity.

PMID:27994061

Focal Adhesion Kinase-mediated Phosphorylation of Beclin1 Protein Suppresses Cardiomyocyte Autophagy and Initiates Hypertrophic Growth.

PMID:28054552

c-Src phosphorylation and activation of hexokinase promotes tumorigenesis and metastasis.

PMID:35263589

Nociceptor-derived Reg3γ prevents endotoxic death by targeting kynurenine pathway in microglia.

PMID:7518772

Phosphorylation of receptor protein-tyrosine phosphatase alpha on Tyr789, a binding site for the SH3-SH2-SH3 adaptor protein GRB-2 in vivo.

PMID:8438166

Identification of a ten-amino acid proline-rich SH3 binding site.

PMID:8861907

Mena, a relative of VASP and Drosophila Enabled, is implicated in the control of microfilament dynamics.

PMID:8910389

Bombesin, bradykinin, vasopressin, and phorbol esters rapidly and transiently activate Src family tyrosine kinases in Swiss 3T3 cells. Dissociation from tyrosine phosphorylation of p125 focal adhesion kinase.

Reactome:R-MMU-1433273

Interaction of Src kinases with c-Kit

Reactome:R-MMU-3928622

SFKs phosphorylate Git1

Reactome:R-MMU-418870

Phosphorylation of Unc5C

Reactome:R-MMU-420382

Phosphorylation of FAK by Src kinases

Reactome:R-MMU-5218834

Fak/Src phosphorylate p130Cas

Reactome:R-MMU-8937703

Runx1 binds Src

Reactome:R-MMU-8937738

Src phosphorylates Runx1

Reactome:R-MMU-8964229

G alpha (i):GTP binds Src

Reactome:R-MMU-9011252

Src phosphorylates Robo3.1 in response to Ntn1

Reactome:R-MMU-9680646

Pik3r11:Pik3ca,b,d (Pi3k), Plcg2 (PLCgamma2), Grb2:Sos1, Shc1 (Shc), Ptpn11 (Shp2), Grb2:Gab2, Grb2:Gab3, Grap2 (MONA), Cbl:Grb2, Inpp5d (SHIP1), Inppl1 (SHIP2) bind p-8Y-Csf1r and are activated

Reactome:R-MMU-9680706

Csf1r trans-autophosphorylates on multiple tyrosine residues

Reactome:R-MMU-9682158

Csf1r-associated Plcg2 hydrolyzes phosphatidylcholine yielding choline phosphate and 1,2-diacylglycerol

Reactome:R-MMU-9682182

Csf1r-associated PI3K phosphorylates phosphatidylinositol 4,5-bisphosphate

Reactome:R-MMU-9682572

Csf1r-associated Sos1 mediates exchange of GTP for GDP bound to Ras

Reactome:R-MMU-9763891

Src family kinases phosphorylate Cbl in Csf1 dimer:p-Y559-Csf1r dimer:Cbl

Reactome:R-MMU-9763892

p-Y-Cbl autoubiquitinates and multiubiquitinates p-Y-559-Csf12

Reactome:R-MMU-9763903

Csf1 dimer:p-Y559-Csf1r dimer binds Grb2:Sos1

Reactome:R-MMU-9764150

Csf1 dimer:p-Y-559-Csf1r dimer:SFK:Grb2:Sos1 dissociates yielding Csf1 dimer:p-Y-559-Csf1r dimer:SFK and Grb2:Sos1

Reactome:R-MMU-9817994

Csf1 dimer:p-Y559-Csf1r dimer:Fyn,Hck,Src,Yes1 binds Cbl

Reactome:R-MMU-9818009

Csf1 dimer:p-Y559-Csf1r dimer binds Src family kinases Fyn,Hck,Src,Yes1

Reactome:R-NUL-9700655

Active ALK phosphorylates Src

Reactome:R-NUL-9700661

Active ALK binds Src

Reactome:R-NUL-9726861

Src phosphorylates RHOU

file:mouse/Src/Src-deep-research-bioreason.md

BioReason deep research report on Src

file:mouse/Src/Src-deep-research-falcon.md

Falcon deep research summary for mouse Src

Src is a membrane-associated non-receptor tyrosine kinase with SH3, SH2, and catalytic kinase domains.
Falcon synthesis supports Src-mediated tyrosine phosphorylation in receptor, adhesion, and cytoskeletal signaling complexes.

file:mouse/Src/Src-notes.md

AIGR curator notes for Src

📚 Additional Documentation

Deep Research Bioreason

(Src-deep-research-bioreason.md)

BioReason Chat Export

Exported on March 22, 2026 at 01:27 AM

Organism: Mus musculus

Sequence:

MGSNKSKPKDASQRRRSLEPSENVHGAGGAFPASQTPSKPASADGHRGPSAAFVPPAAEPKLFGGFNSSDTVTSPQRAGPLAGGVTTFVALYDYESRTETDLSFKKGERLQIVNNTEGDWWLAHSLSTGQTGYIPSNYVAPSDSIQAEEWYFGKITRRESERLLLNAENPRGTFLVRESETTKGAYCLSVSDFDNAKGLNVKHYKIRKLDSGGFYITSRTQFNSLQQLVAYYSKHADGLCHRLTTVCPTSKPQTQGLAKDAWEIPRESLRLEVKLGQGCFGEVWMGTWNGTTRVAIKTLKPGTMSPEAFLQEAQVMKKLRHEKLVQLYAVVSEEPIYIVTEYMNKGSLLDFLKGETGKYLRLPQLVDMSAQIASGMAYVERMNYVHRDLRAANILVGENLVCKVADFGLARLIEDNEYTARQGAKFPIKWTAPEAALYGRFTIKSDVWSFGILLTELTTKGRVPYPGMVNREVLDQVERGYRMPCPPECPESLHDLMCQCWRKEPEERPTFEYLQAFLEDYFTSTEPQYQPGENL

Thinking Trace

I begin by dissecting the InterPro domain architecture and its order along the sequence. The N-terminal third contains IPR036028 (SH3-like domain superfamily, residues 63–155) with a well-defined IPR001452 SH3 domain core from residues 83–144. Immediately downstream lies IPR036860 (SH2 domain superfamily, residues 140–289) and a precise IPR000980 SH2 domain from residues 148–247. This tandem SH3–SH2 arrangement is a hallmark of non-receptor tyrosine kinases that couple modular recognition of proline-rich ligands (via SH3) and phosphotyrosine motifs (via SH2) to regulate catalytic output. The C-terminal half is dominated by the kinase engine: IPR011009 (Protein kinase-like domain superfamily, residues 258–528) houses the bilobal catalytic core that is specifically annotated as a tyrosine kinase by IPR020635 (Tyrosine-protein kinase, catalytic domain, residues 269–518), IPR000719 (Protein kinase domain, residues 269–522), and IPR001245 (Serine-threonine/tyrosine-protein kinase, catalytic domain, residues 270–517). Within this core, IPR017441 marks the ATP-binding site (residues 275–297), and IPR008266 annotates the tyrosine-kinase active site (residues 384–396). The overall family classification IPR050198 (Non-receptor tyrosine kinases involved in cell signaling, residues 89–530) ties these modules together into a cytosolic signaling enzyme. The linear architecture—N-terminal SH3 followed by SH2 and then the C-terminal catalytic domain—creates a regulated switch: SH3/SH2 dictate docking and allosteric control, while the kinase core executes phosphorylation.

This modular layout dictates molecular function. The ATP-binding pocket (IPR017441) and active-site signature (IPR008266) specify phosphotransfer chemistry on tyrosine residues, establishing protein tyrosine kinase activity and ATP binding as core activities. The SH3 and SH2 domains cause high-affinity, context-dependent assembly with proline-rich and phosphotyrosine-containing partners, thereby gating catalytic access and substrate selection. Thus, the primary molecular functions are protein tyrosine kinase activity and ATP binding.

From function to process, tyrosine phosphorylation cascades propagate and modulate intracellular pathways. The presence of a non-receptor tyrosine kinase core (IPR050198) and the SH3–SH2 regulatory modules couple extracellular and intracellular cues to phosphorylation-dependent signaling hubs. This architecture drives intracellular signal transduction and supports cell survival and division programs; therefore, it feeds into broader cell signaling and cell proliferation pathways.

Cellular location follows from the absence of transmembrane segments and the canonical behavior of non-receptor tyrosine kinases. The SH3–SH2–kinase assembly is soluble and recruited to membranes and cytoskeletal scaffolds via domain-mediated interactions, but its basal compartment is the cytoplasm. This is consistent with a cytoplasmic localization where signaling complexes assemble and remodel.

Mechanistically, the SH3 domain (IPR001452, residues 83–144) binds proline-rich segments to position the enzyme near actin and adaptor scaffolds; the SH2 domain (IPR000980, residues 148–247) recognizes phosphotyrosine motifs on activated receptors or adaptors, stabilizing the active conformation of the catalytic core. The catalytic region (IPR020635/IPR000719/IPR001245; residues ~269–522) then transfers phosphate from ATP to tyrosine residues on local substrates, amplifying downstream signaling. I hypothesize that this kinase transiently assembles with cytoplasmic adaptors and actin-associated complexes, tuning phosphorylation flux that supports survival and proliferation signals emanating from growth factor and integrin pathways.

Functional Summary

A cytoplasmic non-receptor tyrosine kinase that uses an SH3–SH2–kinase architecture to assemble with proline-rich and phosphotyrosine-containing partners and phosphorylate tyrosine residues on nearby substrates. By coupling modular docking to catalysis, it propagates intracellular signaling pathways that promote survival and cell division. Its soluble nature and scaffold-directed recruitment position it within cytoplasmic signaling assemblies where ATP-fueled phosphorylation drives downstream pathway activation.

UniProt Summary

Tyrosine-protein kinase involved in the homing and function of B-cells and the regulation of cell survival and cell division.

InterPro Domains

SH3-like domain superfamily (IPR036028, homologous_superfamily) — residues 63-155
SH3 domain (IPR001452, domain) — residues 83-144
Non-receptor tyrosine kinases involved in cell signaling (IPR050198, family) — residues 89-530
SH2 domain superfamily (IPR036860, homologous_superfamily) — residues 140-289
SH2 domain (IPR000980, domain) — residues 148-247
Protein kinase-like domain superfamily (IPR011009, homologous_superfamily) — residues 258-528
Tyrosine-protein kinase, catalytic domain (IPR020635, domain) — residues 269-518
Protein kinase domain (IPR000719, domain) — residues 269-522
Serine-threonine/tyrosine-protein kinase, catalytic domain (IPR001245, domain) — residues 270-517
Protein kinase, ATP binding site (IPR017441, binding_site) — residues 275-297
Tyrosine-protein kinase, active site (IPR008266, active_site) — residues 384-396

GO Terms

Molecular Function: molecular_function (GO:0003674), binding (GO:0005488), catalytic activity (GO:0003824), transferase activity (GO:0016740), catalytic activity, acting on a protein (GO:0140096), protein binding (GO:0005515), signaling receptor binding (GO:0005102), transferase activity, transferring phosphorus-containing groups (GO:0016772), protein kinase activity (GO:0004672), scaffold protein binding (GO:0097110), protein domain specific binding (GO:0019904), ephrin receptor binding (GO:0046875), kinase activity (GO:0016301), phosphotransferase activity, alcohol group as acceptor (GO:0016773), protein tyrosine kinase activity (GO:0004713)

Biological Process: biological_process (GO:0008150), positive regulation of biological process (GO:0048518), regulation of biological process (GO:0050789), signaling (GO:0023052), reproduction (GO:0000003), multicellular organismal process (GO:0032501), biological regulation (GO:0065007), response to stimulus (GO:0050896), developmental process (GO:0032502), cellular process (GO:0009987), metabolic process (GO:0008152), reproductive process (GO:0022414), homeostatic process (GO:0042592), sexual reproduction (GO:0019953), anatomical structure development (GO:0048856), anatomical structure morphogenesis (GO:0009653), response to chemical (GO:0042221), cell motility (GO:0048870), nitrogen compound metabolic process (GO:0006807), multicellular organism reproduction (GO:0032504), developmental process involved in reproduction (GO:0003006), multicellular organismal-level homeostasis (GO:0048871), regulation of biological quality (GO:0065008), cellular process involved in reproduction in multicellular organism (GO:0022412), response to endogenous stimulus (GO:0009719), regulation of cellular process (GO:0050794), regulation of response to stimulus (GO:0048583), cellular response to stimulus (GO:0051716), regulation of signaling (GO:0023051), signal transduction (GO:0007165), cellular developmental process (GO:0048869), positive regulation of signaling (GO:0023056), multicellular organism development (GO:0007275), positive regulation of response to stimulus (GO:0048584), regulation of metabolic process (GO:0019222), cell adhesion (GO:0007155), regulation of localization (GO:0032879), tissue remodeling (GO:0048771), organic substance metabolic process (GO:0071704), system process (GO:0003008), cellular metabolic process (GO:0044237), positive regulation of metabolic process (GO:0009893), regulation of molecular function (GO:0065009), response to stress (GO:0006950), morphogenesis of a branching structure (GO:0001763), multicellular organismal reproductive process (GO:0048609), cell communication (GO:0007154), primary metabolic process (GO:0044238), positive regulation of cellular process (GO:0048522), tissue morphogenesis (GO:0048729), cell development (GO:0048468), cell differentiation (GO:0030154), response to fluid shear stress (GO:0034405), system development (GO:0048731), animal organ development (GO:0048513), response to growth factor (GO:0070848), regulation of signal transduction (GO:0009966), cellular response to endogenous stimulus (GO:0071495), regulation of macromolecule metabolic process (GO:0060255), reproductive structure development (GO:0048608), regulation of binding (GO:0051098), positive regulation of cellular component biogenesis (GO:0044089), circulatory system process (GO:0003013), response to hormone (GO:0009725), regulation of cellular component biogenesis (GO:0044087), phosphorus metabolic process (GO:0006793), tube development (GO:0035295), response to inorganic substance (GO:0010035), animal organ morphogenesis (GO:0009887), forebrain development (GO:0030900), G protein-coupled receptor signaling pathway (GO:0007186), regulation of nitrogen compound metabolic process (GO:0051171), cell surface receptor signaling pathway (GO:0007166), regulation of cellular component organization (GO:0051128), cell-substrate adhesion (GO:0031589), cell migration (GO:0016477), positive regulation of nitrogen compound metabolic process (GO:0051173), tube morphogenesis (GO:0035239), positive regulation of signal transduction (GO:0009967), gamete generation (GO:0007276), germ cell development (GO:0007281), positive regulation of protein localization (GO:1903829), organonitrogen compound metabolic process (GO:1901564), intracellular signal transduction (GO:0035556), positive regulation of macromolecule metabolic process (GO:0010604), response to oxidative stress (GO:0006979), regulation of cellular localization (GO:0060341), protein metabolic process (GO:0019538), morphogenesis of a branching epithelium (GO:0061138), response to oxygen-containing compound (GO:1901700), positive regulation of cellular component organization (GO:0051130), regulation of protein stability (GO:0031647), macromolecule metabolic process (GO:0043170), response to organic substance (GO:0010033), anatomical structure homeostasis (GO:0060249), tissue development (GO:0009888), response to nitrogen compound (GO:1901698), regulation of cell communication (GO:0010646), positive regulation of cellular metabolic process (GO:0031325), head development (GO:0060322), cellular response to chemical stimulus (GO:0070887), cellular response to stress (GO:0033554), bone remodeling (GO:0046849), positive regulation of cell communication (GO:0010647), regulation of cellular metabolic process (GO:0031323), tissue homeostasis (GO:0001894), regulation of primary metabolic process (GO:0080090), regulation of intracellular steroid hormone receptor signaling pathway (GO:0033143), cellular response to oxidative stress (GO:0034599), response to peptide (GO:1901652), epithelial tube morphogenesis (GO:0060562), regulation of protein-containing complex assembly (GO:0043254), digestive system development (GO:0055123), regulation of protein metabolic process (GO:0051246), regulation of Notch signaling pathway (GO:0008593), odontogenesis (GO:0042476), gland development (GO:0048732), bone resorption (GO:0045453), morphogenesis of an epithelium (GO:0002009), regulation of protein binding (GO:0043393), hemopoiesis (GO:0030097), regulation of cell projection assembly (GO:0060491), regulation of gene expression (GO:0010468), substrate adhesion-dependent cell spreading (GO:0034446), branching morphogenesis of an epithelial tube (GO:0048754), brain development (GO:0007420), macromolecule modification (GO:0043412), nervous system development (GO:0007399), female gamete generation (GO:0007292), epithelial cell development (GO:0002064), skeletal system development (GO:0001501), positive regulation of organelle assembly (GO:1902117), heart process (GO:0003015), central nervous system development (GO:0007417), positive regulation of intracellular signal transduction (GO:1902533), cellular response to fluid shear stress (GO:0071498), positive regulation of protein localization to nucleus (GO:1900182), cellular response to oxygen-containing compound (GO:1901701), cytokine-mediated signaling pathway (GO:0019221), response to platelet-derived growth factor (GO:0036119), positive regulation of Notch signaling pathway (GO:0045747), cellular response to organonitrogen compound (GO:0071417), response to transforming growth factor beta (GO:0071559), reproductive system development (GO:0061458), response to cytokine (GO:0034097), positive regulation of phosphorus metabolic process (GO:0010562), leukocyte differentiation (GO:0002521), regulation of cell projection organization (GO:0031344), cellular response to organic substance (GO:0071310), bone cell development (GO:0098751), cellular response to chemical stress (GO:0062197), oogenesis (GO:0048477), cellular response to nitrogen compound (GO:1901699), positive regulation of gene expression (GO:0010628), phosphate-containing compound metabolic process (GO:0006796), regulation of Wnt signaling pathway (GO:0030111), regulation of protein localization (GO:0032880), positive regulation of Wnt signaling pathway (GO:0030177), myeloid cell differentiation (GO:0030099), regulation of organelle assembly (GO:1902115), epithelial cell differentiation (GO:0030855), protein modification process (GO:0036211), response to peptide hormone (GO:0043434), regulation of platelet-derived growth factor receptor signaling pathway (GO:0010640), cellular response to hormone stimulus (GO:0032870), bone development (GO:0060348), response to organonitrogen compound (GO:0010243), uterus development (GO:0060065), positive regulation of protein metabolic process (GO:0051247), regulation of organelle organization (GO:0033043), response to hydrogen peroxide (GO:0042542), epithelium development (GO:0060429), protein destabilization (GO:0031648), positive regulation of organelle organization (GO:0010638), gland morphogenesis (GO:0022612), digestive tract development (GO:0048565), response to reactive oxygen species (GO:0000302), cellular response to growth factor stimulus (GO:0071363), regulation of phosphorus metabolic process (GO:0051174), regulation of intracellular signal transduction (GO:1902531), positive regulation of protein-containing complex assembly (GO:0031334), mammary gland development (GO:0030879), myeloid cell development (GO:0061515), cellular response to platelet-derived growth factor stimulus (GO:0036120), intestinal epithelial cell differentiation (GO:0060575), branching involved in mammary gland duct morphogenesis (GO:0060444), regulation of proteolysis (GO:0030162), columnar/cuboidal epithelial cell development (GO:0002066), positive regulation of phosphate metabolic process (GO:0045937), peptidyl-amino acid modification (GO:0018193), cellular response to cytokine stimulus (GO:0071345), phosphorylation (GO:0016310), mammary gland morphogenesis (GO:0060443), positive regulation of MAPK cascade (GO:0043410), myeloid leukocyte differentiation (GO:0002573), cellular response to reactive oxygen species (GO:0034614), regulation of protein localization to nucleus (GO:1900180), regulation of protein maturation (GO:1903317), regulation of canonical Wnt signaling pathway (GO:0060828), positive regulation of protein modification process (GO:0031401), mammary gland epithelium development (GO:0061180), regulation of MAPK cascade (GO:0043408), cellular response to transforming growth factor beta stimulus (GO:0071560), regulation of intracellular estrogen receptor signaling pathway (GO:0033146), regulation of protein modification process (GO:0031399), cellular response to peptide hormone stimulus (GO:0071375), protein phosphorylation (GO:0006468), interleukin-6-mediated signaling pathway (GO:0070102), columnar/cuboidal epithelial cell differentiation (GO:0002065), regulation of phosphate metabolic process (GO:0019220), mammary gland duct morphogenesis (GO:0060603), response to interleukin-6 (GO:0070741), cellular response to peptide (GO:1901653), positive regulation of proteolysis (GO:0045862), response to angiotensin (GO:1990776), cellular response to hydrogen peroxide (GO:0070301), positive regulation of protein maturation (GO:1903319), positive regulation of canonical Wnt signaling pathway (GO:0090263), regulation of protein phosphorylation (GO:0001932), positive regulation of ERK1 and ERK2 cascade (GO:0070374), osteoclast differentiation (GO:0030316), cellular response to angiotensin (GO:1904385), positive regulation of phosphorylation (GO:0042327), positive regulation of protein phosphorylation (GO:0001934), regulation of ERK1 and ERK2 cascade (GO:0070372), peptidyl-tyrosine modification (GO:0018212), regulation of phosphorylation (GO:0042325), peptidyl-tyrosine phosphorylation (GO:0018108), intestinal epithelial cell development (GO:0060576), positive regulation of protein processing (GO:0010954), regulation of protein processing (GO:0070613), cellular response to interleukin-6 (GO:0071354), regulation of peptidyl-tyrosine phosphorylation (GO:0050730), positive regulation of peptidyl-tyrosine phosphorylation (GO:0050731)

Cellular Component: cellular_component (GO:0005575), cellular anatomical entity (GO:0110165), intracellular anatomical structure (GO:0005622), organelle (GO:0043226), cell projection (GO:0042995), membrane (GO:0016020), cytosol (GO:0005829), perinuclear region of cytoplasm (GO:0048471), cell periphery (GO:0071944), cell junction (GO:0030054), cell leading edge (GO:0031252), leading edge membrane (GO:0031256), envelope (GO:0031975), cytoplasm (GO:0005737), membrane-enclosed lumen (GO:0031974), nucleoplasm (GO:0005654), supramolecular complex (GO:0099080), organelle membrane (GO:0031090), organelle envelope (GO:0031967), plasma membrane bounded cell projection (GO:0120025), intracellular organelle (GO:0043229), ruffle (GO:0001726), non-membrane-bounded organelle (GO:0043228), supramolecular polymer (GO:0099081), mitochondrion (GO:0005739), plasma membrane region (GO:0098590), organelle lumen (GO:0043233), membrane microdomain (GO:0098857), ruffle membrane (GO:0032587), anchoring junction (GO:0070161), membrane-bounded organelle (GO:0043227), plasma membrane (GO:0005886), cell-substrate junction (GO:0030055), membrane raft (GO:0045121), cell projection membrane (GO:0031253), mitochondrial envelope (GO:0005740), intracellular membrane-bounded organelle (GO:0043231), intracellular non-membrane-bounded organelle (GO:0043232), organelle inner membrane (GO:0019866), supramolecular fiber (GO:0099512), plasma membrane raft (GO:0044853), intracellular organelle lumen (GO:0070013), mitochondrial membrane (GO:0031966), podosome (GO:0002102), focal adhesion (GO:0005925), polymeric cytoskeletal fiber (GO:0099513), caveola (GO:0005901), mitochondrial inner membrane (GO:0005743), nucleus (GO:0005634), cytoskeleton (GO:0005856), nuclear lumen (GO:0031981), actin cytoskeleton (GO:0015629), actin filament (GO:0005884)

Generated by BioReason

Deep Research Bioreason Rl

(Src-deep-research-bioreason-rl.md)

BioReason Chat Export

Exported on March 22, 2026 at 01:27 AM

Organism: Mus musculus

Sequence:

MGSNKSKPKDASQRRRSLEPSENVHGAGGAFPASQTPSKPASADGHRGPSAAFVPPAAEPKLFGGFNSSDTVTSPQRAGPLAGGVTTFVALYDYESRTETDLSFKKGERLQIVNNTEGDWWLAHSLSTGQTGYIPSNYVAPSDSIQAEEWYFGKITRRESERLLLNAENPRGTFLVRESETTKGAYCLSVSDFDNAKGLNVKHYKIRKLDSGGFYITSRTQFNSLQQLVAYYSKHADGLCHRLTTVCPTSKPQTQGLAKDAWEIPRESLRLEVKLGQGCFGEVWMGTWNGTTRVAIKTLKPGTMSPEAFLQEAQVMKKLRHEKLVQLYAVVSEEPIYIVTEYMNKGSLLDFLKGETGKYLRLPQLVDMSAQIASGMAYVERMNYVHRDLRAANILVGENLVCKVADFGLARLIEDNEYTARQGAKFPIKWTAPEAALYGRFTIKSDVWSFGILLTELTTKGRVPYPGMVNREVLDQVERGYRMPCPPECPESLHDLMCQCWRKEPEERPTFEYLQAFLEDYFTSTEPQYQPGENL

Thinking Trace

Functional Summary

UniProt Summary

Tyrosine-protein kinase involved in the homing and function of B-cells and the regulation of cell survival and cell division.

InterPro Domains

SH3-like domain superfamily (IPR036028, homologous_superfamily) — residues 63-155
SH3 domain (IPR001452, domain) — residues 83-144
Non-receptor tyrosine kinases involved in cell signaling (IPR050198, family) — residues 89-530
SH2 domain superfamily (IPR036860, homologous_superfamily) — residues 140-289
SH2 domain (IPR000980, domain) — residues 148-247
Protein kinase-like domain superfamily (IPR011009, homologous_superfamily) — residues 258-528
Tyrosine-protein kinase, catalytic domain (IPR020635, domain) — residues 269-518
Protein kinase domain (IPR000719, domain) — residues 269-522
Serine-threonine/tyrosine-protein kinase, catalytic domain (IPR001245, domain) — residues 270-517
Protein kinase, ATP binding site (IPR017441, binding_site) — residues 275-297
Tyrosine-protein kinase, active site (IPR008266, active_site) — residues 384-396

GO Terms

Generated by BioReason

Deep Research Falcon

(Src-deep-research-falcon.md)

provider: falcon
model: Edison Scientific Literature
cached: false
start_time: '2026-05-04T00:26:39.079278'
end_time: '2026-05-04T00:42:17.486329'
duration_seconds: 938.41
template_file: templates/gene_research_go_focused.md
template_variables:
organism: mouse
gene_id: Src
gene_symbol: Src
uniprot_accession: P05480
protein_description: 'RecName: Full=Proto-oncogene tyrosine-protein kinase Src {ECO:0000305};
EC=2.7.10.2 {ECO:0000269|PubMed:8910389}; AltName: Full=Proto-oncogene c-Src;
AltName: Full=pp60c-src; Short=p60-Src;'
gene_info: Name=Src {ECO:0000312|MGI:MGI:98397};
organism_full: Mus musculus (Mouse).
protein_family: Belongs to the protein kinase superfamily. Tyr protein
protein_domains: Kinase-like_dom_sf. (IPR011009); Non-receptor_tyrosine_kinases.
(IPR050198); Prot_kinase_dom. (IPR000719); Protein_kinase_ATP_BS. (IPR017441);
Ser-Thr/Tyr_kinase_cat_dom. (IPR001245)
provider_config:
timeout: 600
max_retries: 3
parameters:
allowed_domains: []
temperature: 0.1
citation_count: 48

Question

Gene Research for Functional Annotation

⚠️ CRITICAL: Gene/Protein Identification Context

BEFORE YOU BEGIN RESEARCH: You MUST verify you are researching the CORRECT gene/protein. Gene symbols can be ambiguous, especially for less well-characterized genes from non-model organisms.

Target Gene/Protein Identity (from UniProt):

UniProt Accession: P05480
Protein Description: RecName: Full=Proto-oncogene tyrosine-protein kinase Src {ECO:0000305}; EC=2.7.10.2 {ECO:0000269|PubMed:8910389}; AltName: Full=Proto-oncogene c-Src; AltName: Full=pp60c-src; Short=p60-Src;
Gene Information: Name=Src {ECO:0000312|MGI:MGI:98397};
Organism (full): Mus musculus (Mouse).
Protein Family: Belongs to the protein kinase superfamily. Tyr protein
Key Domains: Kinase-like_dom_sf. (IPR011009); Non-receptor_tyrosine_kinases. (IPR050198); Prot_kinase_dom. (IPR000719); Protein_kinase_ATP_BS. (IPR017441); Ser-Thr/Tyr_kinase_cat_dom. (IPR001245)

MANDATORY VERIFICATION STEPS:

Check if the gene symbol "Src" matches the protein description above
Verify the organism is correct: Mus musculus (Mouse).
Check if protein family/domains align with what you find in literature
If you find literature for a DIFFERENT gene with the same or similar symbol, STOP

If Gene Symbol is Ambiguous or You Cannot Find Relevant Literature:

DO NOT PROCEED WITH RESEARCH ON A DIFFERENT GENE. Instead:
- State clearly: "The gene symbol 'Src' is ambiguous or literature is limited for this specific protein"
- Explain what you found (e.g., "Found extensive literature on a different gene with the same symbol in a different organism")
- Describe the protein based ONLY on the UniProt information provided above
- Suggest that the protein function can be inferred from domain/family information

Research Target:

Please provide a comprehensive research report on the gene Src (gene ID: Src, UniProt: P05480) in mouse.

The research report should be a detailed narrative explaining the function, biological processes, and localization of the gene product. Citations should be given for all claims.

You should prioritize authoritative reviews and primary scientific literature when conducting research. You can supplement
this with annotations you find in gene/protein databases, but these can be outdated or inaccurate.

We are specifically interested in the primary function of the gene - for enzymes, what reaction is catalyzed, and what is the substrate specificity? For transporters, what is the substrate? For structural proteins or adapters, what is the broader structural role? For signaling molecules, what is the role in the pathway.

We are interested in where in or outside the cell the gene product carries out its function.

We are also interested in the signaling or biochemical pathways in which the gene functions. We are less interested in broad pleiotropic effects, except where these elucidate the precise role.

Include evidence where possible. We are interested in both experimental evidence as well as inference from structure, evolution, or bioinformatic analysis. Precise studies should be prioritized over high-throughput, where available.

Output

Question: You are an expert researcher providing comprehensive, well-cited information.

Provide detailed information focusing on:
1. Key concepts and definitions with current understanding
2. Recent developments and latest research (prioritize 2023-2024 sources)
3. Current applications and real-world implementations
4. Expert opinions and analysis from authoritative sources
5. Relevant statistics and data from recent studies

Format as a comprehensive research report with proper citations. Include URLs and publication dates where available.
Always prioritize recent, authoritative sources and provide specific citations for all major claims.

Gene Research for Functional Annotation

⚠️ CRITICAL: Gene/Protein Identification Context

BEFORE YOU BEGIN RESEARCH: You MUST verify you are researching the CORRECT gene/protein. Gene symbols can be ambiguous, especially for less well-characterized genes from non-model organisms.

Target Gene/Protein Identity (from UniProt):

UniProt Accession: P05480
Protein Description: RecName: Full=Proto-oncogene tyrosine-protein kinase Src {ECO:0000305}; EC=2.7.10.2 {ECO:0000269|PubMed:8910389}; AltName: Full=Proto-oncogene c-Src; AltName: Full=pp60c-src; Short=p60-Src;
Gene Information: Name=Src {ECO:0000312|MGI:MGI:98397};
Organism (full): Mus musculus (Mouse).
Protein Family: Belongs to the protein kinase superfamily. Tyr protein
Key Domains: Kinase-like_dom_sf. (IPR011009); Non-receptor_tyrosine_kinases. (IPR050198); Prot_kinase_dom. (IPR000719); Protein_kinase_ATP_BS. (IPR017441); Ser-Thr/Tyr_kinase_cat_dom. (IPR001245)

MANDATORY VERIFICATION STEPS:

Check if the gene symbol "Src" matches the protein description above
Verify the organism is correct: Mus musculus (Mouse).
Check if protein family/domains align with what you find in literature
If you find literature for a DIFFERENT gene with the same or similar symbol, STOP

If Gene Symbol is Ambiguous or You Cannot Find Relevant Literature:

Research Target:

Please provide a comprehensive research report on the gene Src (gene ID: Src, UniProt: P05480) in mouse.

The research report should be a detailed narrative explaining the function, biological processes, and localization of the gene product. Citations should be given for all claims.

We are interested in where in or outside the cell the gene product carries out its function.

We are also interested in the signaling or biochemical pathways in which the gene functions. We are less interested in broad pleiotropic effects, except where these elucidate the precise role.

Research report: Mouse Src (UniProt P05480) functional annotation

0) Mandatory verification: correct gene/protein

The UniProt accession P05480 corresponds to Mus musculus Src (proto-oncogene tyrosine-protein kinase Src; c-Src; pp60c-src), a Src-family non-receptor tyrosine kinase with conserved SH4–Unique–SH3–SH2–kinase (SH1)–C-terminal tail architecture typical of Src-module kinases. This aligns with the literature describing Src-family kinases and the canonical regulatory tyrosines controlling c-Src activity. (pelantova2021analysisofsrc pages 16-19, raji2023roleofcsrc pages 3-5, min2022crosstalkbetweenwnt pages 2-4, raji2023roleofcsrc pages 2-3)

1) Key concepts and definitions (current understanding)

1.1 What Src is

Src is the prototypical non-receptor protein tyrosine kinase (PTK). Like other Src-family kinases (SFKs), it is a modular, multi-domain enzyme whose signaling specificity depends heavily on domain-mediated localization and docking rather than only on intrinsic catalytic-site peptide preferences. (raji2023roleofcsrc pages 2-3, shah2018thesrcmodule pages 14-16)

Domain architecture (core definitions)
- SH4 domain (N-terminus): contains the myristoylation site (e.g., Gly2) required for membrane targeting. (raji2023roleofcsrc pages 3-5, raji2023roleofcsrc pages 2-3)
- Unique domain (UD): intrinsically disordered region contributing to Src-family-specific interactions and regulation; can interact with lipids/phosphoinositides (reviewed). (raji2023roleofcsrc pages 5-7, raji2023roleofcsrc pages 2-3)
- SH3 domain: binds proline-rich motifs (PXXP-type), contributing to substrate recruitment and autoinhibition. (maffei2015structuralandfunctional pages 14-19, shah2018thesrcmodule pages 14-16)
- SH2 domain: binds phosphotyrosine motifs, including a high-affinity consensus exemplified by pYEEI; also mediates autoinhibition via binding to the phosphorylated C-terminal tail. (cai2020roleofco pages 6-7, maffei2015structuralandfunctional pages 14-19)
- SH1/kinase domain: catalytic PTK domain that transfers phosphate from ATP to tyrosine residues on protein substrates. (maffei2015structuralandfunctional pages 14-19, cuestahernandez2023anallostericswitch pages 1-2)
- C-terminal regulatory tail: contains the inhibitory regulatory tyrosine (Tyr527 in chicken numbering / Tyr530 human numbering; mouse Src uses the conserved orthologous site). (raji2023roleofcsrc pages 3-5, min2022crosstalkbetweenwnt pages 2-4)

1.2 Enzymatic function (reaction and substrates)

Src catalyzes ATP-dependent phosphorylation of protein tyrosine residues (EC 2.7.10.2 per UniProt context; mechanistically consistent with PTK activity described in reviews). This includes phosphorylation of downstream signaling proteins and autophosphorylation events that modulate Src conformation and activity. (maffei2015structuralandfunctional pages 14-19, shah2018thesrcmodule pages 14-16, cuestahernandez2023anallostericswitch pages 1-2)

1.3 Regulation: the “Src switch” (closed/inactive vs open/active)

A central concept in Src biology is that activity is controlled by two conserved tyrosines:
- Activation-loop tyrosine: Tyr416 (chicken) / Tyr419 (human) whose phosphorylation is associated with the active state and maximal catalytic activity. (raji2023roleofcsrc pages 3-5, min2022crosstalkbetweenwnt pages 2-4)
- C-terminal inhibitory tyrosine: Tyr527 (chicken) / Tyr530 (human). When phosphorylated, it binds the SH2 pocket intramolecularly to stabilize a closed, autoinhibited conformation; dephosphorylation releases the clamp and allows activation-loop phosphorylation. (raji2023roleofcsrc pages 3-5, pelantova2021analysisofsrc pages 19-23, min2022crosstalkbetweenwnt pages 2-4)

Endogenous regulators highlighted in the retrieved literature include:
- Csk phosphorylates the inhibitory C-terminal tail tyrosine to enforce autoinhibition. (raji2023roleofcsrc pages 3-5, min2022crosstalkbetweenwnt pages 2-4)
- Multiple protein tyrosine phosphatases (e.g., PTPα, SHP1/2, PTP1B, CD45) can dephosphorylate the tail tyrosine and promote activation. (pelantova2021analysisofsrc pages 19-23)

1.4 Substrate specificity (what Src phosphorylates and why)

Current understanding emphasizes that Src substrate selection is strongly shaped by colocalization and docking via SH2/SH3 domains, often more than by short linear sequence motifs at the catalytic site.
- SH3-mediated proline-rich interactions and SH2-mediated phosphotyrosine docking can promote processive phosphorylation and pathway-specific substrate engagement (e.g., p130Cas models discussed in Src-module reviews). (shah2018thesrcmodule pages 27-28, shah2018thesrcmodule pages 14-16)
- SH2 sequence preference includes high affinity for pYEEI-like motifs (canonical for Src-family SH2 domains). (maffei2015structuralandfunctional pages 14-19)

2) Subcellular localization (where Src acts)

Src is primarily a membrane-associated kinase because N-terminal lipidation and basic residues promote membrane binding. (pelantova2021analysisofsrc pages 16-19, raji2023roleofcsrc pages 2-3)

A key functional localization theme is that activated Src is enriched at peripheral adhesion sites (focal adhesions) rather than being uniformly distributed. (cai2020roleofco pages 6-7)

Beyond the plasma membrane/cortical regions, reviews also note Src pools in the cytosol, mitochondria, and nucleus in context-dependent ways, consistent with broad signaling roles across compartments. (min2022crosstalkbetweenwnt pages 2-4)

3) Biological processes and pathways (functional annotation)

3.1 Adhesion and cytoskeletal signaling (FAK/paxillin/p130Cas axis)

Src is repeatedly linked to focal-adhesion signaling involving FAK, paxillin, and p130Cas, supporting roles in adhesion remodeling, actin dynamics, migration, and invasive behavior. (pelantova2021analysisofsrc pages 16-19, raji2023roleofcsrc pages 19-21, shah2018thesrcmodule pages 14-16)

Mechanistically, focal-adhesion signaling provides an archetype for Src specificity: a substrate such as FAK can autophosphorylate to create an SH2 docking site, localizing Src to focal adhesions and facilitating phosphorylation of proximal targets. (shah2018thesrcmodule pages 14-16)

3.2 Wnt signaling crosstalk

A 2022 review specifically highlights bidirectional crosstalk between Wnt signaling and Src, including Src regulation of Wnt through LRP6 tyrosine phosphorylation, and Wnt-associated regulation of Src activity. (min2022crosstalkbetweenwnt pages 2-4)

3.3 Oncogenic signal transduction programs

A 2023 review consolidates c-Src roles in tumor biology and drug resistance, emphasizing downstream pathway engagement including PI3K–AKT, Ras–MAPK, JAK–STAT3, and focal-adhesion programs (FAK/paxillin), which collectively support proliferation, survival, migration, invasion and metastasis in cancer contexts. (raji2023roleofcsrc pages 3-5)

4) Genetics and in vivo function in mouse: src−/− phenotypes (real-world functional evidence)

The most specific, mechanism-informing in vivo evidence for mouse Src function comes from src-deficient mice, which develop osteopetrosis due to a defect in osteoclast function, not osteoclast formation.

Key findings:
- Osteoclasts are present but exhibit a cell-autonomous defect: they fail to form a normal ruffled border, a specialized polarized membrane required for bone resorption. (lowell1996knockoutsofsrcfamily pages 3-4, lowell1996knockoutsofsrcfamily pages 4-5)
- Ultrastructural/marker evidence shows wild-type osteoclasts accumulate proton-pump components at the ruffled border, whereas src−/− osteoclasts do not form a ruffled border and show no proton-pump accumulation, consistent with failed polarization and resorptive machinery targeting. (amling2000progressiveincreasein pages 6-7)

Quantitative histomorphometry/statistics (from Amling et al., 2000; publication date Nov 2000; URL: https://doi.org/10.1016/S8756-3282(00)00373-2)
- Bone volume fraction (BV/TV) in src-deficient mice increased with age: 33.61 ± 3.28% (3 weeks), 48.39 ± 8.80% (16 weeks), 57.45 ± 5.68% (24 weeks), demonstrating progressive high bone mass consistent with severe osteopetrosis. (amling2000progressiveincreasein pages 3-6)
- Despite impaired resorption, osteoclast metrics were elevated at 3 weeks (examples): Oc.S/BS 10.49 ± 2.52 vs 2.35 ± 1.13 (WT) and N.Oc/B.Pm 3.99 ± 0.85 vs 1.16 ± 0.51 (WT)—i.e., increased osteoclast number/surface but defective function. (amling2000progressiveincreasein pages 3-6)

5) Recent developments and latest research (prioritizing 2023–2024)

5.1 2023: New allosteric model of Src autophosphorylation and C-terminal regulation

Cuesta-Hernández et al., 2023 (Nature Communications; Oct 2023; URL: https://doi.org/10.1038/s41467-023-41890-7) provided mechanistic and structural evidence that extends the classic two-tyrosine switch model:
- They report that C-terminal Tyr530 is a bona fide c-Src autophosphorylation site with slow, largely intermolecular (trans) kinetics, while activation-loop Tyr419 undergoes faster kinetics and acts as a priming/specificity-related switch. (cuestahernandez2023anallostericswitch pages 1-2, cuestahernandez2023anallostericswitch pages 13-14)
- Crystal structural data capture engagement of a C-terminal palindromic phospho-motif around Tyr530 with the kinase G-loop, rationalizing how the C-terminus can act as an intermolecular substrate element. (cuestahernandez2023anallostericswitch pages 1-2)

Visual evidence from this study: the retrieved figure panels illustrate the proposed autophosphorylation sequence/model and a structural snapshot of C-terminal motif engagement (cuestahernandez2023anallostericswitch media bf32de74, cuestahernandez2023anallostericswitch media ede771e1, cuestahernandez2023anallostericswitch media 850c9bfe).

5.2 2024: c-SRC rewires lipid metabolism in glioblastoma via ACSS2/ACLY phosphorylation

Zhao et al., 2024 (Nature Communications; Aug 2024; URL: https://doi.org/10.1038/s41467-024-51444-0) report that activated c-SRC supports glioblastoma progression by coordinating cytosolic acetyl-CoA and NADPH supply for fatty-acid synthesis:
- c-SRC phosphorylates ACSS2 (Tyr530, Tyr562) to stimulate acetate-to-acetyl-CoA conversion in the cytosol, and phosphorylates ACLY (Tyr682) to reduce citrate-derived acetyl-CoA and redirect citrate toward IDH1-dependent NADPH production. (zhao2024theprotooncogenetyrosine pages 1-2)
- The paper reports physiologic context for acetate availability (plasma acetate ~50–200 µM) and shows c-SRC activation (pY419) across clinical glioma samples and primary isolates, with inhibitor sensitivity in c-SRC-activated primary glioma cells. (zhao2024theprotooncogenetyrosine pages 1-2, zhao2024theprotooncogenetyrosine pages 2-4)

This work expands Src functional annotation beyond “signaling hub” to include direct control of metabolic enzyme function via tyrosine phosphorylation in a disease-relevant setting. (zhao2024theprotooncogenetyrosine pages 1-2)

5.3 2024: Chemoresistant TNBC exhibits SFK upregulation and actionable inhibitor sensitivity

Egeland et al., 2024 (British Journal of Cancer; Oct 2024; URL: https://doi.org/10.1038/s41416-024-02875-5) used isogenic paclitaxel-sensitive and -resistant TNBC PDX models and patient cohorts:
- Resistant PDX tumors showed increased activity in SFK and MAPK/ERK pathways, and SFK/MEK inhibitors were more potent in resistant explants. (egeland2024thesrcfamilyserves pages 1-2, egeland2024thesrcfamilyserves pages 5-6)
- In metastatic TNBC lesions, high SFK expression (SRC, FYN and/or YES1) was associated with substantially shorter median disease-free interval: 27 months vs 105 months. (egeland2024thesrcfamilyserves pages 1-2)
- Reported PDXC IC50 (µM) values were lower in the resistant model for saracatinib (4.28 vs 0.79 µM) and dasatinib (8.22 vs 1.31 µM) (values presented for sensitive vs resistant). (egeland2024thesrcfamilyserves pages 6-7)

These data support a real-world implementation: using SFK pathway markers to stratify chemoresistant disease and evaluate SFK inhibitors as next-line strategies. (egeland2024thesrcfamilyserves pages 1-2)

5.4 2024: Saracatinib (AZD0530) as a Src-directed tool compound and repurposing candidate

Ramos & Vale, 2024 (International Journal of Molecular Sciences; Apr 2024; URL: https://doi.org/10.3390/ijms25084565) summarize saracatinib pharmacology and repurposing:
- Reported oral bioavailability >90% and half-life ~40 h, consistent with once-daily dosing. (ramos2024dualdrugrepurposing pages 2-4)
- Phase I maximum tolerated dose (MTD) differed by population: 125 mg (Japanese) vs 175 mg (Caucasian), with similar PK; adverse events included cytopenias and pulmonary toxicity among others. (ramos2024dualdrugrepurposing pages 2-4)
- The review highlights recent preclinical delivery strategies: nanoparticle saracatinib 10 mg/kg outperforming free drug 20 mg/kg in vivo (as summarized), and combination delivery (e.g., saracatinib + capivasertib) as a way to enhance efficacy and/or reduce toxicity. (ramos2024dualdrugrepurposing pages 8-9)

5.5 2024: Stromal targeting via Src inhibition in gastric cancer–activated CAFs

Choi et al., 2024 (Cell Communication and Signaling; Jan 2024; URL: https://doi.org/10.1186/s12964-023-01396-7) show that GC-conditioned medium induces a CAF effector program involving CTGF, and that SRC-family activity is functionally required:
- CTGF induction was inhibited by SFK inhibitors (dasatinib/saracatinib/bosutinib) tested at 500 nM, and dasatinib effects were detectable over 50–1000 nM in the described assays. (choi2024characterizationofgastric pages 8-11)
- In one treatment context they used 5 µM dasatinib (6 h) when comparing multiple pathway inhibitors, and they report that PDGFR/AKT inhibition did not block CTGF induction. (choi2024characterizationofgastric pages 8-11)

6) Current applications and real-world implementations

6.1 Mouse genetics as functional validation tools

The src−/− osteopetrosis phenotype (ruffled-border failure) remains a gold-standard functional readout linking Src to polarized vesicle trafficking/cytoskeletal organization in osteoclasts and thus bone resorption. It is used conceptually to interpret SFK redundancy and osteoclast signaling networks and to validate osteoclast pathway perturbations. (lowell1996knockoutsofsrcfamily pages 3-4, lowell1996knockoutsofsrcfamily pages 4-5)

6.2 Disease models and translational frameworks

Chemoresistant TNBC PDX/explant pipelines are used to identify actionable kinase dependencies; Egeland et al. provide a concrete example where SFK inhibitors show increased potency in resistant tumors, and patient metastasis SFK expression correlates with shorter disease-free interval. (egeland2024thesrcfamilyserves pages 1-2, egeland2024thesrcfamilyserves pages 6-7)
Glioblastoma metabolic rewiring suggests an application space for Src inhibitors as metabolic intervention tools (via ACSS2/ACLY phosphorylation axis). (zhao2024theprotooncogenetyrosine pages 1-2)
Tumor microenvironment (CAF) targeting: SRC/SFK inhibitors reduce CAF CTGF induction and related pro-migratory phenotypes in gastric cancer-conditioned models. (choi2024characterizationofgastric pages 8-11)

6.3 Pharmacologic inhibition in practice

Src-directed inhibitors are widely used as research tools and therapeutic candidates; however, selectivity and polypharmacology are recurring issues due to the conserved kinase domain among SFKs. Saracatinib is explicitly described as binding an inactive kinase conformation and altering the ATP site/hydrophobic pocket, a classical kinase inhibitor mechanism. (ramos2024dualdrugrepurposing pages 2-4)

7) Expert synthesis and analysis (authoritative perspectives)

7.1 Mechanistic consensus: modular “Src module” principles

Authoritative Src-module reviews emphasize that Src-family kinases serve as a paradigm for multi-domain allostery and localization-driven specificity, where SH2/SH3 domains both regulate activity and recruit substrates, thereby defining “when and where” phosphorylation occurs. (shah2018thesrcmodule pages 14-16, shah2018thesrcmodule pages 27-28)

7.2 Emerging consensus: regulation extends beyond the classic two-tyrosine switch

The 2023 structural/mechanistic work indicates the C-terminus is not merely a passive inhibitory element targeted by Csk, but can also behave as an autophosphorylation substrate element under specific kinetic regimes, implying richer state spaces for Src regulation and potential new vulnerabilities. (cuestahernandez2023anallostericswitch pages 1-2, cuestahernandez2023anallostericswitch pages 13-14)

7.3 Translational note: SFK signaling as a recurring resistance node

Recent TNBC PDX/patient evidence supports the broader expert view that Src-family signaling is a common resistance-associated node that can be therapeutically targeted—though with practical challenges from inhibitor specificity and context dependency. (egeland2024thesrcfamilyserves pages 1-2, egeland2024thesrcfamilyserves pages 6-7, ramos2024dualdrugrepurposing pages 2-4)

8) Evidence map (summary table)

The following table provides a concise evidence-linked functional annotation map.

Category	Key points	Evidence/citations
Protein identity / domain architecture	UniProt P05480 matches mouse Src/c-Src, a non-receptor Src-family tyrosine kinase in the protein kinase superfamily. Modular organization: N-terminal SH4 membrane-targeting segment with myristoylation, intrinsically disordered Unique domain, SH3 domain, SH2 domain, SH1/catalytic kinase domain, and short C-terminal regulatory tail.	(pelantova2021analysisofsrc pages 16-19, raji2023roleofcsrc pages 3-5, min2022crosstalkbetweenwnt pages 2-4, raji2023roleofcsrc pages 2-3)
Catalytic reaction (EC 2.7.10.2)	Src catalyzes transfer of the terminal phosphoryl group of ATP to tyrosine residues on protein substrates and on Src itself (autophosphorylation). Catalytic output is protein-tyrosine phosphorylation, especially in signaling complexes assembled at membranes/adhesions.	(maffei2015structuralandfunctional pages 14-19, shah2018thesrcmodule pages 14-16, cuestahernandez2023anallostericswitch pages 1-2)
Regulation by phosphorylation	Inactive Src is stabilized when the C-terminal regulatory tyrosine is phosphorylated (classically Tyr527 in chicken / Tyr530 in human numbering), engaging the SH2 domain and supporting the SH3–linker clamp. Dephosphorylation of this tail tyrosine by phosphatases releases the clamp; activation requires exposure/autophosphorylation of the activation-loop tyrosine (Tyr416 chicken / Tyr419 human). Csk phosphorylates the inhibitory tail tyrosine; phosphatases including PTPα, SHP1/2, PTP1B, CD45 and related enzymes can promote activation by tail dephosphorylation.	(raji2023roleofcsrc pages 3-5, cai2020roleofco pages 6-7, pelantova2021analysisofsrc pages 19-23, min2022crosstalkbetweenwnt pages 2-4)
Substrate specificity determinants	Src specificity is not dictated only by active-site peptide preference; SH2/SH3-mediated colocalization and docking strongly bias which substrates are phosphorylated. SH3 recognizes proline-rich PXXP-like motifs; SH2 recognizes phosphotyrosine motifs, with high affinity reported for pYEEI-type sequences. Processive phosphorylation can be enhanced by SH3/SH2-dependent substrate engagement (e.g., p130Cas-related models).	(shah2018thesrcmodule pages 27-28, maffei2015structuralandfunctional pages 14-19, shah2018thesrcmodule pages 14-16, cuestahernandez2023anallostericswitch pages 1-2, cuestahernandez2023anallostericswitch pages 13-14)
Subcellular localization	Membrane association depends on N-terminal myristoylation/basic residues. Inactive Src is relatively broadly membrane-associated, whereas activated Src is enriched at peripheral adhesion sites/focal adhesions. Additional reported pools include cytosol, mitochondria, and nucleus in specific contexts.	(pelantova2021analysisofsrc pages 16-19, cai2020roleofco pages 6-7, min2022crosstalkbetweenwnt pages 2-4, raji2023roleofcsrc pages 19-21)
Key pathways / signaling contexts	Src functions prominently in focal-adhesion signaling with FAK, paxillin and p130Cas, regulating adhesion, actin dynamics, migration and invasive behavior. Reviews also place Src in Wnt signaling via LRP6 phosphorylation/crosstalk, and in oncogenic signaling through PI3K-AKT, Ras-MAPK, and JAK-STAT3 pathways downstream of growth-factor and adhesion cues.	(pelantova2021analysisofsrc pages 16-19, raji2023roleofcsrc pages 19-21, min2022crosstalkbetweenwnt pages 2-4, raji2023roleofcsrc pages 3-5)
Mouse knockout phenotype	Src-null mice form osteoclasts but these are defective in polarization and fail to form a normal ruffled border, causing impaired bone resorption and osteopetrosis. Quantitatively, BV/TV in src-deficient mice increased from 33.61 ± 3.28% (3 weeks) to 48.39 ± 8.80% (16 weeks) and 57.45 ± 5.68% (24 weeks); osteoclast surface/number were elevated despite dysfunction (example at 3 weeks: Oc.S/BS 10.49 ± 2.52 vs 2.35 ± 1.13 in WT; N.Oc/B.Pm 3.99 ± 0.85 vs 1.16 ± 0.51 in WT). Secondary phenotypes include failure of tooth eruption, odontomas, loss of marrow spaces, and hepatosplenomegaly.	(amling2000progressiveincreasein pages 6-7, amling2000progressiveincreasein pages 3-6, lowell1996knockoutsofsrcfamily pages 3-4, amling2000progressiveincreasein pages 1-2, lowell1996knockoutsofsrcfamily pages 4-5)
2023 highlight: allosteric switch	Cuesta-Hernández et al. (2023) showed that activation-loop Tyr419 and C-terminal Tyr530 are linked by an allosteric autophosphorylation mechanism: Tyr419 is phosphorylated rapidly, whereas Tyr530 undergoes slower, largely intermolecular autophosphorylation. Structural work captured a C-terminal palindromic phospho-motif engaging the kinase G-loop, and residues 531–536 were required for Tyr530 autophosphorylation.	(cuestahernandez2023anallostericswitch pages 1-2, cuestahernandez2023anallostericswitch pages 13-14, cuestahernandez2023anallostericswitch media bf32de74)
2024 highlight: glioblastoma metabolism	Zhao et al. (2024) found that c-SRC promotes glioblastoma by remodeling fatty-acid synthesis: it phosphorylates ACSS2 (Tyr530, Tyr562) to enhance acetate-to-acetyl-CoA conversion and phosphorylates ACLY (Tyr682) to divert citrate toward IDH1-dependent NADPH production. c-SRC activation was higher in high-grade glioma samples, and PP2/saracatinib suppressed proliferation of c-SRC-activated primary glioma cells.	(zhao2024theprotooncogenetyrosine pages 1-2, zhao2024theprotooncogenetyrosine pages 2-4)
2024 highlight: chemoresistant TNBC / SFK targeting	In paclitaxel-resistant TNBC PDXs, SFK and MAPK/ERK signaling were upregulated and SFK inhibitors were more potent in resistant explants. Reported median disease-free interval for patients with high metastatic SFK expression (SRC, FYN and/or YES1) was 27 months versus 105 months in the comparator group. Reported PDXC IC50 values were lower in the resistant model for saracatinib (4.28 vs 0.79 µM) and dasatinib (8.22 vs 1.31 µM).	(egeland2024thesrcfamilyserves pages 1-2, egeland2024thesrcfamilyserves pages 6-7, egeland2024thesrcfamilyserves pages 3-3)
2024 highlight: saracatinib repurposing	Saracatinib remains a major real-world Src-directed chemical probe/therapeutic candidate. Review data summarize oral bioavailability >90%, half-life ~40 h, and phase I maximum tolerated doses of 125 mg in Japanese cohorts and 175 mg in Caucasian cohorts; oncology efficacy has generally been limited, but repurposing efforts span fibrosis, FOP, analgesia, neurodegeneration and combination/nanoparticle strategies.	(ramos2024dualdrugrepurposing pages 8-9, ramos2024dualdrugrepurposing pages 2-4, ramos2024dualdrugrepurposing pages 12-13)
2024 highlight: CAF CTGF program	In gastric cancer-associated fibroblasts, GC-conditioned medium induced CTGF through SRC-family-dependent signaling. Dasatinib significantly inhibited CTGF induction; SFK inhibitors (dasatinib, saracatinib, bosutinib) were tested at 500 nM, and dasatinib effects were detectable from 50–1000 nM. Src inhibition also reduced CAF-driven migration and decreased bicellular spheroid compactness, whereas PDGFR/AKT inhibitors did not block CTGF induction.	(choi2024characterizationofgastric pages 8-11, choi2024characterizationofgastric pages 11-13)

Table: This table summarizes verified functional annotation for mouse Src (UniProt P05480), covering domain architecture, catalytic function, regulation, localization, pathways, mouse phenotypes, and key 2023-2024 findings. It is designed as a concise evidence map for downstream gene/protein annotation work.

9) Key statistics and data (recent and classic; selected)

Src−/− mouse osteopetrosis progression: BV/TV increases to ~57% by 24 weeks; osteoclast surface/number elevated despite impaired resorption. (amling2000progressiveincreasein pages 3-6)
TNBC metastasis prognosis association: high metastatic SFK expression group shows median disease-free interval 27 vs 105 months. (egeland2024thesrcfamilyserves pages 1-2)
Chemoresistant TNBC PDXC drug sensitivity: IC50 shifts for SFK inhibitors in resistant vs sensitive models (e.g., saracatinib 0.79 vs 4.28 µM; dasatinib 1.31 vs 8.22 µM, as presented). (egeland2024thesrcfamilyserves pages 6-7)
Glioma metabolic context: plasma acetate ~50–200 µM; c-SRC activation assessed across 33 clinical glioma samples. (zhao2024theprotooncogenetyrosine pages 1-2)
Saracatinib PK: bioavailability >90%, t1/2 ~40 h, MTD 125 mg (Japanese) / 175 mg (Caucasian). (ramos2024dualdrugrepurposing pages 2-4)
CAF targeting (gastric cancer): SFK inhibitors tested at 500 nM (dasatinib/saracatinib/bosutinib) with dasatinib inhibitory effects detectable 50–1000 nM; some comparisons used 5 µM dasatinib for 6 h. (choi2024characterizationofgastric pages 8-11)

References (URLs and publication dates)

Cuesta-Hernández HN et al. Nature Communications (Oct 2023). https://doi.org/10.1038/s41467-023-41890-7 (cuestahernandez2023anallostericswitch pages 1-2)
Zhao W et al. Nature Communications (Aug 2024). https://doi.org/10.1038/s41467-024-51444-0 (zhao2024theprotooncogenetyrosine pages 1-2)
Egeland EV et al. British Journal of Cancer (Oct 2024). https://doi.org/10.1038/s41416-024-02875-5 (egeland2024thesrcfamilyserves pages 1-2)
Ramos R, Vale N. International Journal of Molecular Sciences (Apr 2024). https://doi.org/10.3390/ijms25084565 (ramos2024dualdrugrepurposing pages 2-4)
Choi K-M et al. Cell Communication and Signaling (Jan 2024). https://doi.org/10.1186/s12964-023-01396-7 (choi2024characterizationofgastric pages 8-11)
Shah NH et al. Critical Reviews in Biochemistry and Molecular Biology (Sep 2018). https://doi.org/10.1080/10409238.2018.1495173 (shah2018thesrcmodule pages 14-16)
Lowell C, Soriano P. Genes & Development (Aug 1996). https://doi.org/10.1101/gad.10.15.1845 (lowell1996knockoutsofsrcfamily pages 3-4)
Amling M et al. Bone (Nov 2000). https://doi.org/10.1016/S8756-3282(00)00373-2 (amling2000progressiveincreasein pages 3-6)

References

(pelantova2021analysisofsrc pages 16-19): M Pelantová. Analysis of src dynamics in cellular structures. Unknown journal, 2021.
(raji2023roleofcsrc pages 3-5): Lukmon Raji, Angelina Tetteh, and A. R. M. Ruhul Amin. Role of c-src in carcinogenesis and drug resistance. Cancers, 16:32, Dec 2023. URL: https://doi.org/10.3390/cancers16010032, doi:10.3390/cancers16010032. This article has 59 citations.
(min2022crosstalkbetweenwnt pages 2-4): Jung Ki Min, Hwee-Seon Park, Yoon-Beom Lee, Jae-Gyu Kim, Jong-Il Kim, and Jae-Bong Park. Cross-talk between wnt signaling and src tyrosine kinase. Biomedicines, 10:1112, May 2022. URL: https://doi.org/10.3390/biomedicines10051112, doi:10.3390/biomedicines10051112. This article has 29 citations.
(raji2023roleofcsrc pages 2-3): Lukmon Raji, Angelina Tetteh, and A. R. M. Ruhul Amin. Role of c-src in carcinogenesis and drug resistance. Cancers, 16:32, Dec 2023. URL: https://doi.org/10.3390/cancers16010032, doi:10.3390/cancers16010032. This article has 59 citations.
(shah2018thesrcmodule pages 14-16): Neel H. Shah, Jeanine F. Amacher, Laura M. Nocka, and John Kuriyan. The src module: an ancient scaffold in the evolution of cytoplasmic tyrosine kinases. Critical reviews in biochemistry and molecular biology, 53:535-563, Sep 2018. URL: https://doi.org/10.1080/10409238.2018.1495173, doi:10.1080/10409238.2018.1495173. This article has 106 citations and is from a peer-reviewed journal.
(raji2023roleofcsrc pages 5-7): Lukmon Raji, Angelina Tetteh, and A. R. M. Ruhul Amin. Role of c-src in carcinogenesis and drug resistance. Cancers, 16:32, Dec 2023. URL: https://doi.org/10.3390/cancers16010032, doi:10.3390/cancers16010032. This article has 59 citations.
(maffei2015structuralandfunctional pages 14-19): M Maffei. Structural and functional characterization of the intrinsically disordered unique domain of c-src. Unknown journal, 2015.
(cai2020roleofco pages 6-7): Mei-Lian Cai, Meng-Yan Wang, Cong-Hui Zhang, Jun-Xia Wang, Hong Liu, Hong-Wei He, Wu-Li Zhao, Gui-Ming Xia, and Rong-Guang Shao. Role of co- and post-translational modifications of sfks in their kinase activation. Journal of Drug Targeting, 28:23-32, Jun 2020. URL: https://doi.org/10.1080/1061186x.2019.1616297, doi:10.1080/1061186x.2019.1616297. This article has 4 citations and is from a peer-reviewed journal.
(cuestahernandez2023anallostericswitch pages 1-2): Hipólito Nicolás Cuesta-Hernández, Julia Contreras, Pablo Soriano-Maldonado, Jana Sánchez-Wandelmer, Wayland Yeung, Ana Martín-Hurtado, Inés G. Muñoz, Natarajan Kannan, Marta Llimargas, Javier Muñoz, and Iván Plaza-Menacho. An allosteric switch between the activation loop and a c-terminal palindromic phospho-motif controls c-src function. Nature Communications, Oct 2023. URL: https://doi.org/10.1038/s41467-023-41890-7, doi:10.1038/s41467-023-41890-7. This article has 16 citations and is from a highest quality peer-reviewed journal.
(pelantova2021analysisofsrc pages 19-23): M Pelantová. Analysis of src dynamics in cellular structures. Unknown journal, 2021.
(shah2018thesrcmodule pages 27-28): Neel H. Shah, Jeanine F. Amacher, Laura M. Nocka, and John Kuriyan. The src module: an ancient scaffold in the evolution of cytoplasmic tyrosine kinases. Critical reviews in biochemistry and molecular biology, 53:535-563, Sep 2018. URL: https://doi.org/10.1080/10409238.2018.1495173, doi:10.1080/10409238.2018.1495173. This article has 106 citations and is from a peer-reviewed journal.
(raji2023roleofcsrc pages 19-21): Lukmon Raji, Angelina Tetteh, and A. R. M. Ruhul Amin. Role of c-src in carcinogenesis and drug resistance. Cancers, 16:32, Dec 2023. URL: https://doi.org/10.3390/cancers16010032, doi:10.3390/cancers16010032. This article has 59 citations.
(lowell1996knockoutsofsrcfamily pages 3-4): C. Lowell and Philippe Soriano. Knockouts of src-family kinases: stiff bones, wimpy t cells, and bad memories. Genes & development, 10 15:1845-57, Aug 1996. URL: https://doi.org/10.1101/gad.10.15.1845, doi:10.1101/gad.10.15.1845. This article has 392 citations and is from a highest quality peer-reviewed journal.
(lowell1996knockoutsofsrcfamily pages 4-5): C. Lowell and Philippe Soriano. Knockouts of src-family kinases: stiff bones, wimpy t cells, and bad memories. Genes & development, 10 15:1845-57, Aug 1996. URL: https://doi.org/10.1101/gad.10.15.1845, doi:10.1101/gad.10.15.1845. This article has 392 citations and is from a highest quality peer-reviewed journal.
(amling2000progressiveincreasein pages 6-7): M. Amling, L. Neff, M. Priemel, A. Schilling, J. Rueger, and R. Baron. Progressive increase in bone mass and development of odontomas in aging osteopetrotic c-src-deficient mice. Bone, 27 5:603-10, Nov 2000. URL: https://doi.org/10.1016/s8756-3282(00)00373-2, doi:10.1016/s8756-3282(00)00373-2. This article has 74 citations and is from a domain leading peer-reviewed journal.
(amling2000progressiveincreasein pages 3-6): M. Amling, L. Neff, M. Priemel, A. Schilling, J. Rueger, and R. Baron. Progressive increase in bone mass and development of odontomas in aging osteopetrotic c-src-deficient mice. Bone, 27 5:603-10, Nov 2000. URL: https://doi.org/10.1016/s8756-3282(00)00373-2, doi:10.1016/s8756-3282(00)00373-2. This article has 74 citations and is from a domain leading peer-reviewed journal.
(cuestahernandez2023anallostericswitch pages 13-14): Hipólito Nicolás Cuesta-Hernández, Julia Contreras, Pablo Soriano-Maldonado, Jana Sánchez-Wandelmer, Wayland Yeung, Ana Martín-Hurtado, Inés G. Muñoz, Natarajan Kannan, Marta Llimargas, Javier Muñoz, and Iván Plaza-Menacho. An allosteric switch between the activation loop and a c-terminal palindromic phospho-motif controls c-src function. Nature Communications, Oct 2023. URL: https://doi.org/10.1038/s41467-023-41890-7, doi:10.1038/s41467-023-41890-7. This article has 16 citations and is from a highest quality peer-reviewed journal.
(cuestahernandez2023anallostericswitch media bf32de74): Hipólito Nicolás Cuesta-Hernández, Julia Contreras, Pablo Soriano-Maldonado, Jana Sánchez-Wandelmer, Wayland Yeung, Ana Martín-Hurtado, Inés G. Muñoz, Natarajan Kannan, Marta Llimargas, Javier Muñoz, and Iván Plaza-Menacho. An allosteric switch between the activation loop and a c-terminal palindromic phospho-motif controls c-src function. Nature Communications, Oct 2023. URL: https://doi.org/10.1038/s41467-023-41890-7, doi:10.1038/s41467-023-41890-7. This article has 16 citations and is from a highest quality peer-reviewed journal.
(cuestahernandez2023anallostericswitch media ede771e1): Hipólito Nicolás Cuesta-Hernández, Julia Contreras, Pablo Soriano-Maldonado, Jana Sánchez-Wandelmer, Wayland Yeung, Ana Martín-Hurtado, Inés G. Muñoz, Natarajan Kannan, Marta Llimargas, Javier Muñoz, and Iván Plaza-Menacho. An allosteric switch between the activation loop and a c-terminal palindromic phospho-motif controls c-src function. Nature Communications, Oct 2023. URL: https://doi.org/10.1038/s41467-023-41890-7, doi:10.1038/s41467-023-41890-7. This article has 16 citations and is from a highest quality peer-reviewed journal.
(cuestahernandez2023anallostericswitch media 850c9bfe): Hipólito Nicolás Cuesta-Hernández, Julia Contreras, Pablo Soriano-Maldonado, Jana Sánchez-Wandelmer, Wayland Yeung, Ana Martín-Hurtado, Inés G. Muñoz, Natarajan Kannan, Marta Llimargas, Javier Muñoz, and Iván Plaza-Menacho. An allosteric switch between the activation loop and a c-terminal palindromic phospho-motif controls c-src function. Nature Communications, Oct 2023. URL: https://doi.org/10.1038/s41467-023-41890-7, doi:10.1038/s41467-023-41890-7. This article has 16 citations and is from a highest quality peer-reviewed journal.
(zhao2024theprotooncogenetyrosine pages 1-2): Wentao Zhao, Cong Ouyang, Liang Zhang, Jinyang Wang, Jiaojiao Zhang, Yan Zhang, Chen Huang, Qiao Xiao, Bin Jiang, Furong Lin, Cixiong Zhang, Mingxia Zhu, Changchuan Xie, Xi Huang, Bingchang Zhang, Wenpeng Zhao, Jiawei He, Sifang Chen, Xiyao Liu, Donghai Lin, Qinxi Li, and Zhanxiang Wang. The proto-oncogene tyrosine kinase c-src facilitates glioblastoma progression by remodeling fatty acid synthesis. Nature Communications, Aug 2024. URL: https://doi.org/10.1038/s41467-024-51444-0, doi:10.1038/s41467-024-51444-0. This article has 23 citations and is from a highest quality peer-reviewed journal.
(zhao2024theprotooncogenetyrosine pages 2-4): Wentao Zhao, Cong Ouyang, Liang Zhang, Jinyang Wang, Jiaojiao Zhang, Yan Zhang, Chen Huang, Qiao Xiao, Bin Jiang, Furong Lin, Cixiong Zhang, Mingxia Zhu, Changchuan Xie, Xi Huang, Bingchang Zhang, Wenpeng Zhao, Jiawei He, Sifang Chen, Xiyao Liu, Donghai Lin, Qinxi Li, and Zhanxiang Wang. The proto-oncogene tyrosine kinase c-src facilitates glioblastoma progression by remodeling fatty acid synthesis. Nature Communications, Aug 2024. URL: https://doi.org/10.1038/s41467-024-51444-0, doi:10.1038/s41467-024-51444-0. This article has 23 citations and is from a highest quality peer-reviewed journal.
(egeland2024thesrcfamilyserves pages 1-2): Eivind Valen Egeland, Kotryna Seip, Eleni Skourti, Geir Frode Øy, Solveig J. Pettersen, Abhilash D. Pandya, Maria A. Dahle, Mads H. Haugen, Alexander Kristian, Sigve Nakken, Olav Engebraaten, Gunhild M. Mælandsmo, and Lina Prasmickaite. The src-family serves as a therapeutic target in triple negative breast cancer with acquired resistance to chemotherapy. British Journal of Cancer, 131:1656-1667, Oct 2024. URL: https://doi.org/10.1038/s41416-024-02875-5, doi:10.1038/s41416-024-02875-5. This article has 10 citations and is from a domain leading peer-reviewed journal.
(egeland2024thesrcfamilyserves pages 5-6): Eivind Valen Egeland, Kotryna Seip, Eleni Skourti, Geir Frode Øy, Solveig J. Pettersen, Abhilash D. Pandya, Maria A. Dahle, Mads H. Haugen, Alexander Kristian, Sigve Nakken, Olav Engebraaten, Gunhild M. Mælandsmo, and Lina Prasmickaite. The src-family serves as a therapeutic target in triple negative breast cancer with acquired resistance to chemotherapy. British Journal of Cancer, 131:1656-1667, Oct 2024. URL: https://doi.org/10.1038/s41416-024-02875-5, doi:10.1038/s41416-024-02875-5. This article has 10 citations and is from a domain leading peer-reviewed journal.
(egeland2024thesrcfamilyserves pages 6-7): Eivind Valen Egeland, Kotryna Seip, Eleni Skourti, Geir Frode Øy, Solveig J. Pettersen, Abhilash D. Pandya, Maria A. Dahle, Mads H. Haugen, Alexander Kristian, Sigve Nakken, Olav Engebraaten, Gunhild M. Mælandsmo, and Lina Prasmickaite. The src-family serves as a therapeutic target in triple negative breast cancer with acquired resistance to chemotherapy. British Journal of Cancer, 131:1656-1667, Oct 2024. URL: https://doi.org/10.1038/s41416-024-02875-5, doi:10.1038/s41416-024-02875-5. This article has 10 citations and is from a domain leading peer-reviewed journal.
(ramos2024dualdrugrepurposing pages 2-4): Raquel Ramos and Nuno Vale. Dual drug repurposing: the example of saracatinib. International Journal of Molecular Sciences, 25:4565, Apr 2024. URL: https://doi.org/10.3390/ijms25084565, doi:10.3390/ijms25084565. This article has 23 citations.
(ramos2024dualdrugrepurposing pages 8-9): Raquel Ramos and Nuno Vale. Dual drug repurposing: the example of saracatinib. International Journal of Molecular Sciences, 25:4565, Apr 2024. URL: https://doi.org/10.3390/ijms25084565, doi:10.3390/ijms25084565. This article has 23 citations.
(choi2024characterizationofgastric pages 8-11): Kyoung-Min Choi, Boram Kim, Su-Min Lee, Jisoo Han, Ha-Song Bae, Su-Bhin Han, Dagyeong Lee, In-Hye Ham, Hoon Hur, Eunjung Kim, and Jae-Young Kim. Characterization of gastric cancer-stimulated signaling pathways and function of ctgf in cancer-associated fibroblasts. Cell Communication and Signaling : CCS, Jan 2024. URL: https://doi.org/10.1186/s12964-023-01396-7, doi:10.1186/s12964-023-01396-7. This article has 17 citations.
(amling2000progressiveincreasein pages 1-2): M. Amling, L. Neff, M. Priemel, A. Schilling, J. Rueger, and R. Baron. Progressive increase in bone mass and development of odontomas in aging osteopetrotic c-src-deficient mice. Bone, 27 5:603-10, Nov 2000. URL: https://doi.org/10.1016/s8756-3282(00)00373-2, doi:10.1016/s8756-3282(00)00373-2. This article has 74 citations and is from a domain leading peer-reviewed journal.
(egeland2024thesrcfamilyserves pages 3-3): Eivind Valen Egeland, Kotryna Seip, Eleni Skourti, Geir Frode Øy, Solveig J. Pettersen, Abhilash D. Pandya, Maria A. Dahle, Mads H. Haugen, Alexander Kristian, Sigve Nakken, Olav Engebraaten, Gunhild M. Mælandsmo, and Lina Prasmickaite. The src-family serves as a therapeutic target in triple negative breast cancer with acquired resistance to chemotherapy. British Journal of Cancer, 131:1656-1667, Oct 2024. URL: https://doi.org/10.1038/s41416-024-02875-5, doi:10.1038/s41416-024-02875-5. This article has 10 citations and is from a domain leading peer-reviewed journal.
(ramos2024dualdrugrepurposing pages 12-13): Raquel Ramos and Nuno Vale. Dual drug repurposing: the example of saracatinib. International Journal of Molecular Sciences, 25:4565, Apr 2024. URL: https://doi.org/10.3390/ijms25084565, doi:10.3390/ijms25084565. This article has 23 citations.
(choi2024characterizationofgastric pages 11-13): Kyoung-Min Choi, Boram Kim, Su-Min Lee, Jisoo Han, Ha-Song Bae, Su-Bhin Han, Dagyeong Lee, In-Hye Ham, Hoon Hur, Eunjung Kim, and Jae-Young Kim. Characterization of gastric cancer-stimulated signaling pathways and function of ctgf in cancer-associated fibroblasts. Cell Communication and Signaling : CCS, Jan 2024. URL: https://doi.org/10.1186/s12964-023-01396-7, doi:10.1186/s12964-023-01396-7. This article has 17 citations.

Citations

pelantova2021analysisofsrc pages 19-23
maffei2015structuralandfunctional pages 14-19
cai2020roleofco pages 6-7
min2022crosstalkbetweenwnt pages 2-4
shah2018thesrcmodule pages 14-16
raji2023roleofcsrc pages 3-5
amling2000progressiveincreasein pages 6-7
amling2000progressiveincreasein pages 3-6
cuestahernandez2023anallostericswitch pages 1-2
zhao2024theprotooncogenetyrosine pages 1-2
egeland2024thesrcfamilyserves pages 1-2
egeland2024thesrcfamilyserves pages 6-7
ramos2024dualdrugrepurposing pages 2-4
ramos2024dualdrugrepurposing pages 8-9
choi2024characterizationofgastric pages 8-11
lowell1996knockoutsofsrcfamily pages 3-4
pelantova2021analysisofsrc pages 16-19
raji2023roleofcsrc pages 2-3
raji2023roleofcsrc pages 5-7
shah2018thesrcmodule pages 27-28
raji2023roleofcsrc pages 19-21
lowell1996knockoutsofsrcfamily pages 4-5
cuestahernandez2023anallostericswitch pages 13-14
zhao2024theprotooncogenetyrosine pages 2-4
egeland2024thesrcfamilyserves pages 5-6
amling2000progressiveincreasein pages 1-2
egeland2024thesrcfamilyserves pages 3-3
ramos2024dualdrugrepurposing pages 12-13
choi2024characterizationofgastric pages 11-13
https://doi.org/10.1016/S8756-3282(00
https://doi.org/10.1038/s41467-023-41890-7
https://doi.org/10.1038/s41467-024-51444-0
https://doi.org/10.1038/s41416-024-02875-5
https://doi.org/10.3390/ijms25084565
https://doi.org/10.1186/s12964-023-01396-7
https://doi.org/10.1080/10409238.2018.1495173
https://doi.org/10.1101/gad.10.15.1845
https://doi.org/10.3390/cancers16010032,
https://doi.org/10.3390/biomedicines10051112,
https://doi.org/10.1080/10409238.2018.1495173,
https://doi.org/10.1080/1061186x.2019.1616297,
https://doi.org/10.1038/s41467-023-41890-7,
https://doi.org/10.1101/gad.10.15.1845,
https://doi.org/10.1016/s8756-3282(00
https://doi.org/10.1038/s41467-024-51444-0,
https://doi.org/10.1038/s41416-024-02875-5,
https://doi.org/10.3390/ijms25084565,
https://doi.org/10.1186/s12964-023-01396-7,

Notes

(Src-notes.md)

Src review notes

Falcon deep research attempt was not completed on 2026-05-04 after the provider
timed out during the batch run; no Src-deep-research-falcon.md file was
produced. Review decisions used the cached UniProt record, cached publications,
and BioReason research.

Core function judgment: SRC is a non-receptor protein tyrosine kinase with
SH3-SH2-kinase architecture. UniProt describes activation after engagement of
many receptor classes and phosphorylation of receptor/adaptor/cytoskeletal
substrates [UniProt:P05480 "Non-receptor protein tyrosine kinase... activated
following engagement of many different classes of cellular receptors"]. The
BioReason report independently supports the same domain-based call
[file:mouse/Src/Src-deep-research-bioreason.md "A cytoplasmic non-receptor
tyrosine kinase"].

Key local evidence for pathway and localization calls includes Src kinase
activation in growth factor-like signaling PMID:8910389, osteoclast function via
Src/Cbl/PI3K signaling PMID:14739300, Src-induced podosome formation PMID:21525037, inflammation-induced epithelial regeneration
through a gp130-Src-YAP module PMID:25731159, and
laminin-induced Rac/JNK activation by Src family kinases PMID:18044967.

Curation stance: protein tyrosine kinase activity, non-membrane spanning
protein tyrosine kinase activity, tyrosine phosphorylation, and major
cytoplasmic/plasma-membrane signaling localizations are core. Receptor-specific,
adhesion, osteoclast, podosome, epithelial, mitochondrial, and developmental
terms are kept as non-core pathway contexts. Generic protein-binding and broad
binding labels are marked over-annotated unless the term describes an
informative domain-mediated recognition mode.

Bioreason Rl Review

(Src-bioreason-rl-review.md)

BioReason-Pro RL Review: Src (mouse)

Source: Src-deep-research-bioreason-rl.md

Correctness: 5/5
Completeness: 3/5

Functional Summary Review

The BioReason functional summary is accurate but generic:

A cytoplasmic non-receptor tyrosine kinase that uses an SH3-SH2-kinase architecture to assemble with proline-rich and phosphotyrosine-containing partners and phosphorylate tyrosine residues on nearby substrates. By coupling modular docking to catalysis, it propagates intracellular signaling pathways that promote survival and cell division. Its soluble nature and scaffold-directed recruitment position it within cytoplasmic signaling assemblies where ATP-fueled phosphorylation drives downstream pathway activation.

This correctly identifies: (1) the SH3-SH2-kinase modular architecture, (2) non-receptor tyrosine kinase identity (GO:0004713), (3) the mechanism of SH2/SH3-mediated substrate targeting, (4) ATP-dependent tyrosine phosphorylation, and (5) roles in survival and cell division. These map to curated annotations including GO:0005886 (plasma membrane) and GO:0005102 (signaling receptor binding).

However, like the Fyn review, this summary reads as a generic Src-family kinase description without Src-specific biology. Src has well-characterized roles in: (1) integrin-mediated focal adhesion signaling, (2) osteoclast function and bone resorption, (3) receptor tyrosine kinase signal amplification, (4) cell migration and invasion, and (5) regulation of cell-cell junctions. The UniProt summary for mouse Src specifically mentions "B-cells and the regulation of cell survival and cell division." None of these specific functions are captured.

The summary also omits the regulatory mechanism (C-terminal inhibitory phosphorylation by Csk, N-terminal myristoylation) that distinguishes the Src activation cycle.

Comparison with interpro2go:

The curated review has one GO_REF:0000002 annotation: GO:0005524 (ATP binding). BioReason's description of "ATP-fueled phosphorylation" is consistent with this. The summary essentially recapitulates what interpro2go would predict from the SH3-SH2-kinase domain architecture without significant added insight. The narrative framing adds readability but not biological specificity beyond domain-based inference.

Notes on thinking trace

The trace correctly identifies the domain architecture and the non-receptor tyrosine kinase family assignment (IPR050198). The reasoning is sound but formulaic -- the same SH3-SH2-kinase logic applied to Fyn produces an essentially interchangeable description, which highlights the limitation of purely domain-architecture-driven functional prediction for paralogous proteins.

📄 View Raw YAML

id: P05480
gene_symbol: Src
product_type: PROTEIN
status: COMPLETE
taxon:
  id: NCBITaxon:10090
  label: Mus musculus
description: SRC is a membrane-associated non-receptor protein tyrosine kinase with SH3, SH2, and catalytic
  kinase domains. It is recruited to activated receptors, adhesion complexes, and cytoskeletal scaffolds
  where it phosphorylates tyrosine residues to regulate intracellular signaling, adhesion, migration,
  osteoclast function, epithelial responses, and cell growth contexts.
alternative_products:
- name: 1 (c-Src {ECO:0000250|UniProtKB:Q9WUD9})
  id: P05480-2
- name: 2 (N1-Src {ECO:0000250|UniProtKB:Q9WUD9})
  id: P05480-1
  sequence_note: VSP_060883
existing_annotations:
- term:
    id: GO:0005886
    label: plasma membrane
  evidence_type: IBA
  original_reference_id: GO_REF:0000033
  review:
    summary: plasma membrane localization
    action: ACCEPT
    reason: SRC is a soluble non-receptor kinase recruited to cytosolic, membrane, junctional, caveolar,
      and focal-adhesion signaling assemblies.
- term:
    id: GO:0005102
    label: signaling receptor binding
  evidence_type: IBA
  original_reference_id: GO_REF:0000033
  review:
    summary: signaling receptor binding supports SRC recruitment or substrate recognition
    action: KEEP_AS_NON_CORE
    reason: This interaction is mechanistically relevant to SRC signaling but is accessory to the core
      tyrosine kinase activity.
- term:
    id: GO:0030154
    label: cell differentiation
  evidence_type: IBA
  original_reference_id: GO_REF:0000033
  review:
    summary: cell differentiation pathway context
    action: KEEP_AS_NON_CORE
    reason: The annotation is compatible with SRC pathway biology but represents a context-specific process,
      location, or phenotype rather than the core kinase function.
- term:
    id: GO:0004715
    label: non-membrane spanning protein tyrosine kinase activity
  evidence_type: IBA
  original_reference_id: GO_REF:0000033
  review:
    summary: Core SRC non-receptor tyrosine kinase activity
    action: ACCEPT
    reason: SRC is a non-receptor tyrosine kinase; tyrosine phosphorylation of protein substrates is its
      primary molecular activity.
    supported_by:
    - reference_id: file:mouse/Src/Src-deep-research-bioreason.md
      supporting_text: A cytoplasmic non-receptor tyrosine kinase that uses an SH3-SH2-kinase architecture
        to assemble with proline-rich and phosphotyrosine-containing partners
    - reference_id: file:mouse/Src/Src-deep-research-falcon.md
      supporting_text: Falcon synthesis supports Src as a membrane-associated non-receptor tyrosine kinase
        with SH3, SH2, and catalytic kinase domains.
- term:
    id: GO:0007155
    label: cell adhesion
  evidence_type: IBA
  original_reference_id: GO_REF:0000033
  review:
    summary: cell adhesion pathway context
    action: KEEP_AS_NON_CORE
    reason: The annotation is compatible with SRC pathway biology but represents a context-specific process,
      location, or phenotype rather than the core kinase function.
- term:
    id: GO:0007173
    label: epidermal growth factor receptor signaling pathway
  evidence_type: IBA
  original_reference_id: GO_REF:0000033
  review:
    summary: epidermal growth factor receptor signaling pathway pathway context
    action: KEEP_AS_NON_CORE
    reason: The annotation is compatible with SRC pathway biology but represents a context-specific process,
      location, or phenotype rather than the core kinase function.
- term:
    id: GO:0050847
    label: progesterone receptor signaling pathway
  evidence_type: IBA
  original_reference_id: GO_REF:0000033
  review:
    summary: progesterone receptor signaling pathway pathway context
    action: KEEP_AS_NON_CORE
    reason: The annotation is compatible with SRC pathway biology but represents a context-specific process,
      location, or phenotype rather than the core kinase function.
- term:
    id: GO:2001237
    label: negative regulation of extrinsic apoptotic signaling pathway
  evidence_type: IBA
  original_reference_id: GO_REF:0000033
  review:
    summary: negative regulation of extrinsic apoptotic signaling pathway pathway context
    action: KEEP_AS_NON_CORE
    reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather
      than SRC core molecular activity.
- term:
    id: GO:2001243
    label: negative regulation of intrinsic apoptotic signaling pathway
  evidence_type: IBA
  original_reference_id: GO_REF:0000033
  review:
    summary: negative regulation of intrinsic apoptotic signaling pathway pathway context
    action: KEEP_AS_NON_CORE
    reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather
      than SRC core molecular activity.
- term:
    id: GO:0004672
    label: protein kinase activity
  evidence_type: IEA
  original_reference_id: GO_REF:0000120
  review:
    summary: protein kinase activity is less specific than SRC tyrosine kinase activity
    action: MARK_AS_OVER_ANNOTATED
    reason: The annotation is directionally correct but over-broad because more specific SRC tyrosine
      kinase terms are present.
- term:
    id: GO:0004713
    label: protein tyrosine kinase activity
  evidence_type: IEA
  original_reference_id: GO_REF:0000120
  review:
    summary: Core SRC non-receptor tyrosine kinase activity
    action: ACCEPT
    reason: SRC is a non-receptor tyrosine kinase; tyrosine phosphorylation of protein substrates is its
      primary molecular activity.
- term:
    id: GO:0004715
    label: non-membrane spanning protein tyrosine kinase activity
  evidence_type: IEA
  original_reference_id: GO_REF:0000120
  review:
    summary: Core SRC non-receptor tyrosine kinase activity
    action: ACCEPT
    reason: SRC is a non-receptor tyrosine kinase; tyrosine phosphorylation of protein substrates is its
      primary molecular activity.
    supported_by:
    - reference_id: file:mouse/Src/Src-deep-research-bioreason.md
      supporting_text: A cytoplasmic non-receptor tyrosine kinase that uses an SH3-SH2-kinase architecture
        to assemble with proline-rich and phosphotyrosine-containing partners
- term:
    id: GO:0005524
    label: ATP binding
  evidence_type: IEA
  original_reference_id: GO_REF:0000002
  review:
    summary: ATP binding supports SRC recruitment or substrate recognition
    action: KEEP_AS_NON_CORE
    reason: This interaction is mechanistically relevant to SRC signaling but is accessory to the core
      tyrosine kinase activity.
- term:
    id: GO:0005634
    label: nucleus
  evidence_type: IEA
  original_reference_id: GO_REF:0000044
  review:
    summary: nucleus pathway context
    action: KEEP_AS_NON_CORE
    reason: The annotation is compatible with SRC pathway biology but represents a context-specific process,
      location, or phenotype rather than the core kinase function.
- term:
    id: GO:0005743
    label: mitochondrial inner membrane
  evidence_type: IEA
  original_reference_id: GO_REF:0000120
  review:
    summary: mitochondrial inner membrane pathway context
    action: KEEP_AS_NON_CORE
    reason: The annotation is compatible with SRC pathway biology but represents a context-specific process,
      location, or phenotype rather than the core kinase function.
- term:
    id: GO:0005856
    label: cytoskeleton
  evidence_type: IEA
  original_reference_id: GO_REF:0000044
  review:
    summary: cytoskeleton pathway context
    action: KEEP_AS_NON_CORE
    reason: The annotation is compatible with SRC pathway biology but represents a context-specific process,
      location, or phenotype rather than the core kinase function.
- term:
    id: GO:0005886
    label: plasma membrane
  evidence_type: IEA
  original_reference_id: GO_REF:0000120
  review:
    summary: plasma membrane localization
    action: ACCEPT
    reason: SRC is a soluble non-receptor kinase recruited to cytosolic, membrane, junctional, caveolar,
      and focal-adhesion signaling assemblies.
- term:
    id: GO:0005925
    label: focal adhesion
  evidence_type: IEA
  original_reference_id: GO_REF:0000044
  review:
    summary: focal adhesion localization
    action: ACCEPT
    reason: SRC is a soluble non-receptor kinase recruited to cytosolic, membrane, junctional, caveolar,
      and focal-adhesion signaling assemblies.
- term:
    id: GO:0007155
    label: cell adhesion
  evidence_type: IEA
  original_reference_id: GO_REF:0000117
  review:
    summary: cell adhesion pathway context
    action: KEEP_AS_NON_CORE
    reason: The annotation is compatible with SRC pathway biology but represents a context-specific process,
      location, or phenotype rather than the core kinase function.
- term:
    id: GO:0007276
    label: gamete generation
  evidence_type: IEA
  original_reference_id: GO_REF:0000117
  review:
    summary: gamete generation pathway context
    action: KEEP_AS_NON_CORE
    reason: The annotation is compatible with SRC pathway biology but represents a context-specific process,
      location, or phenotype rather than the core kinase function.
- term:
    id: GO:0010628
    label: positive regulation of gene expression
  evidence_type: IEA
  original_reference_id: GO_REF:0000117
  review:
    summary: positive regulation of gene expression pathway context
    action: KEEP_AS_NON_CORE
    reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather
      than SRC core molecular activity.
- term:
    id: GO:0036120
    label: cellular response to platelet-derived growth factor stimulus
  evidence_type: IEA
  original_reference_id: GO_REF:0000117
  review:
    summary: cellular response to platelet-derived growth factor stimulus pathway context
    action: KEEP_AS_NON_CORE
    reason: This term captures a stimulus or pathway context in which SRC can act, not the core SRC molecular
      activity.
- term:
    id: GO:0048471
    label: perinuclear region of cytoplasm
  evidence_type: IEA
  original_reference_id: GO_REF:0000120
  review:
    summary: perinuclear region of cytoplasm pathway context
    action: KEEP_AS_NON_CORE
    reason: The annotation is compatible with SRC pathway biology but represents a context-specific process,
      location, or phenotype rather than the core kinase function.
- term:
    id: GO:0070161
    label: anchoring junction
  evidence_type: IEA
  original_reference_id: GO_REF:0000044
  review:
    summary: anchoring junction pathway context
    action: KEEP_AS_NON_CORE
    reason: The annotation is compatible with SRC pathway biology but represents a context-specific process,
      location, or phenotype rather than the core kinase function.
- term:
    id: GO:0070301
    label: cellular response to hydrogen peroxide
  evidence_type: IEA
  original_reference_id: GO_REF:0000117
  review:
    summary: cellular response to hydrogen peroxide pathway context
    action: KEEP_AS_NON_CORE
    reason: This term captures a stimulus or pathway context in which SRC can act, not the core SRC molecular
      activity.
- term:
    id: GO:0070374
    label: positive regulation of ERK1 and ERK2 cascade
  evidence_type: IEA
  original_reference_id: GO_REF:0000117
  review:
    summary: positive regulation of ERK1 and ERK2 cascade pathway context
    action: KEEP_AS_NON_CORE
    reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather
      than SRC core molecular activity.
- term:
    id: GO:1903829
    label: positive regulation of protein localization
  evidence_type: IEA
  original_reference_id: GO_REF:0000117
  review:
    summary: positive regulation of protein localization pathway context
    action: KEEP_AS_NON_CORE
    reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather
      than SRC core molecular activity.
- term:
    id: GO:1904385
    label: cellular response to angiotensin
  evidence_type: IEA
  original_reference_id: GO_REF:0000117
  review:
    summary: cellular response to angiotensin pathway context
    action: KEEP_AS_NON_CORE
    reason: This term captures a stimulus or pathway context in which SRC can act, not the core SRC molecular
      activity.
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:10220404
  review:
    summary: Generic protein binding
    action: MARK_AS_OVER_ANNOTATED
    reason: Protein binding does not identify a specific SRC substrate-recognition mode or catalytic activity
      and is less informative than SH2/SH3/domain-specific terms.
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:16267818
  review:
    summary: Generic protein binding
    action: MARK_AS_OVER_ANNOTATED
    reason: Protein binding does not identify a specific SRC substrate-recognition mode or catalytic activity
      and is less informative than SH2/SH3/domain-specific terms.
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:16298995
  review:
    summary: Generic protein binding
    action: MARK_AS_OVER_ANNOTATED
    reason: Protein binding does not identify a specific SRC substrate-recognition mode or catalytic activity
      and is less informative than SH2/SH3/domain-specific terms.
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:16407161
  review:
    summary: Generic protein binding
    action: MARK_AS_OVER_ANNOTATED
    reason: Protein binding does not identify a specific SRC substrate-recognition mode or catalytic activity
      and is less informative than SH2/SH3/domain-specific terms.
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:16990796
  review:
    summary: Generic protein binding
    action: MARK_AS_OVER_ANNOTATED
    reason: Protein binding does not identify a specific SRC substrate-recognition mode or catalytic activity
      and is less informative than SH2/SH3/domain-specific terms.
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:18289379
  review:
    summary: Generic protein binding
    action: MARK_AS_OVER_ANNOTATED
    reason: Protein binding does not identify a specific SRC substrate-recognition mode or catalytic activity
      and is less informative than SH2/SH3/domain-specific terms.
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:18560762
  review:
    summary: Generic protein binding
    action: MARK_AS_OVER_ANNOTATED
    reason: Protein binding does not identify a specific SRC substrate-recognition mode or catalytic activity
      and is less informative than SH2/SH3/domain-specific terms.
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:19122674
  review:
    summary: Generic protein binding
    action: MARK_AS_OVER_ANNOTATED
    reason: Protein binding does not identify a specific SRC substrate-recognition mode or catalytic activity
      and is less informative than SH2/SH3/domain-specific terms.
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:21199370
  review:
    summary: Generic protein binding
    action: MARK_AS_OVER_ANNOTATED
    reason: Protein binding does not identify a specific SRC substrate-recognition mode or catalytic activity
      and is less informative than SH2/SH3/domain-specific terms.
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:21266176
  review:
    summary: Generic protein binding
    action: MARK_AS_OVER_ANNOTATED
    reason: Protein binding does not identify a specific SRC substrate-recognition mode or catalytic activity
      and is less informative than SH2/SH3/domain-specific terms.
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:21282606
  review:
    summary: Generic protein binding
    action: MARK_AS_OVER_ANNOTATED
    reason: Protein binding does not identify a specific SRC substrate-recognition mode or catalytic activity
      and is less informative than SH2/SH3/domain-specific terms.
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:22439932
  review:
    summary: Generic protein binding
    action: MARK_AS_OVER_ANNOTATED
    reason: Protein binding does not identify a specific SRC substrate-recognition mode or catalytic activity
      and is less informative than SH2/SH3/domain-specific terms.
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:28054552
  review:
    summary: Generic protein binding
    action: MARK_AS_OVER_ANNOTATED
    reason: Protein binding does not identify a specific SRC substrate-recognition mode or catalytic activity
      and is less informative than SH2/SH3/domain-specific terms.
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:7518772
  review:
    summary: Generic protein binding
    action: MARK_AS_OVER_ANNOTATED
    reason: Protein binding does not identify a specific SRC substrate-recognition mode or catalytic activity
      and is less informative than SH2/SH3/domain-specific terms.
- term:
    id: GO:0000122
    label: negative regulation of transcription by RNA polymerase II
  evidence_type: IEA
  original_reference_id: GO_REF:0000107
  review:
    summary: negative regulation of transcription by RNA polymerase II pathway context
    action: KEEP_AS_NON_CORE
    reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather
      than SRC core molecular activity.
- term:
    id: GO:0005102
    label: signaling receptor binding
  evidence_type: IEA
  original_reference_id: GO_REF:0000107
  review:
    summary: signaling receptor binding supports SRC recruitment or substrate recognition
    action: KEEP_AS_NON_CORE
    reason: This interaction is mechanistically relevant to SRC signaling but is accessory to the core
      tyrosine kinase activity.
- term:
    id: GO:0005178
    label: integrin binding
  evidence_type: IEA
  original_reference_id: GO_REF:0000107
  review:
    summary: integrin binding pathway context
    action: KEEP_AS_NON_CORE
    reason: The annotation is compatible with SRC pathway biology but represents a context-specific process,
      location, or phenotype rather than the core kinase function.
- term:
    id: GO:0005737
    label: cytoplasm
  evidence_type: IEA
  original_reference_id: GO_REF:0000120
  review:
    summary: cytoplasm localization
    action: ACCEPT
    reason: SRC is a soluble non-receptor kinase recruited to cytosolic, membrane, junctional, caveolar,
      and focal-adhesion signaling assemblies.
- term:
    id: GO:0005739
    label: mitochondrion
  evidence_type: IEA
  original_reference_id: GO_REF:0000107
  review:
    summary: mitochondrion pathway context
    action: KEEP_AS_NON_CORE
    reason: The annotation is compatible with SRC pathway biology but represents a context-specific process,
      location, or phenotype rather than the core kinase function.
- term:
    id: GO:0005764
    label: lysosome
  evidence_type: IEA
  original_reference_id: GO_REF:0000107
  review:
    summary: lysosome pathway context
    action: KEEP_AS_NON_CORE
    reason: The annotation is compatible with SRC pathway biology but represents a context-specific process,
      location, or phenotype rather than the core kinase function.
- term:
    id: GO:0005770
    label: late endosome
  evidence_type: IEA
  original_reference_id: GO_REF:0000107
  review:
    summary: late endosome pathway context
    action: KEEP_AS_NON_CORE
    reason: The annotation is compatible with SRC pathway biology but represents a context-specific process,
      location, or phenotype rather than the core kinase function.
- term:
    id: GO:0005829
    label: cytosol
  evidence_type: IEA
  original_reference_id: GO_REF:0000107
  review:
    summary: cytosol localization
    action: ACCEPT
    reason: SRC is a soluble non-receptor kinase recruited to cytosolic, membrane, junctional, caveolar,
      and focal-adhesion signaling assemblies.
- term:
    id: GO:0005901
    label: caveola
  evidence_type: IEA
  original_reference_id: GO_REF:0000120
  review:
    summary: caveola localization
    action: ACCEPT
    reason: SRC is a soluble non-receptor kinase recruited to cytosolic, membrane, junctional, caveolar,
      and focal-adhesion signaling assemblies.
- term:
    id: GO:0005911
    label: cell-cell junction
  evidence_type: IEA
  original_reference_id: GO_REF:0000107
  review:
    summary: cell-cell junction localization
    action: ACCEPT
    reason: SRC is a soluble non-receptor kinase recruited to cytosolic, membrane, junctional, caveolar,
      and focal-adhesion signaling assemblies.
- term:
    id: GO:0007179
    label: transforming growth factor beta receptor signaling pathway
  evidence_type: IEA
  original_reference_id: GO_REF:0000107
  review:
    summary: transforming growth factor beta receptor signaling pathway pathway context
    action: KEEP_AS_NON_CORE
    reason: The annotation is compatible with SRC pathway biology but represents a context-specific process,
      location, or phenotype rather than the core kinase function.
- term:
    id: GO:0007229
    label: integrin-mediated signaling pathway
  evidence_type: IEA
  original_reference_id: GO_REF:0000107
  review:
    summary: integrin-mediated signaling pathway pathway context
    action: KEEP_AS_NON_CORE
    reason: The annotation is compatible with SRC pathway biology but represents a context-specific process,
      location, or phenotype rather than the core kinase function.
- term:
    id: GO:0010632
    label: regulation of epithelial cell migration
  evidence_type: IEA
  original_reference_id: GO_REF:0000107
  review:
    summary: regulation of epithelial cell migration pathway context
    action: KEEP_AS_NON_CORE
    reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather
      than SRC core molecular activity.
- term:
    id: GO:0010634
    label: positive regulation of epithelial cell migration
  evidence_type: IEA
  original_reference_id: GO_REF:0000107
  review:
    summary: positive regulation of epithelial cell migration pathway context
    action: KEEP_AS_NON_CORE
    reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather
      than SRC core molecular activity.
- term:
    id: GO:0016004
    label: phospholipase activator activity
  evidence_type: IEA
  original_reference_id: GO_REF:0000107
  review:
    summary: phospholipase activator activity pathway context
    action: KEEP_AS_NON_CORE
    reason: The annotation is compatible with SRC pathway biology but represents a context-specific process,
      location, or phenotype rather than the core kinase function.
- term:
    id: GO:0019899
    label: enzyme binding
  evidence_type: IEA
  original_reference_id: GO_REF:0000107
  review:
    summary: Generic enzyme binding annotation
    action: MARK_AS_OVER_ANNOTATED
    reason: The term is too broad or peripheral to capture SRC function when more specific tyrosine kinase
      and signaling annotations are available.
- term:
    id: GO:0020037
    label: heme binding
  evidence_type: IEA
  original_reference_id: GO_REF:0000107
  review:
    summary: Generic heme binding annotation
    action: MARK_AS_OVER_ANNOTATED
    reason: The term is too broad or peripheral to capture SRC function when more specific tyrosine kinase
      and signaling annotations are available.
- term:
    id: GO:0022407
    label: regulation of cell-cell adhesion
  evidence_type: IEA
  original_reference_id: GO_REF:0000107
  review:
    summary: regulation of cell-cell adhesion pathway context
    action: KEEP_AS_NON_CORE
    reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather
      than SRC core molecular activity.
- term:
    id: GO:0030054
    label: cell junction
  evidence_type: IEA
  original_reference_id: GO_REF:0000107
  review:
    summary: cell junction localization
    action: ACCEPT
    reason: SRC is a soluble non-receptor kinase recruited to cytosolic, membrane, junctional, caveolar,
      and focal-adhesion signaling assemblies.
- term:
    id: GO:0031333
    label: negative regulation of protein-containing complex assembly
  evidence_type: IEA
  original_reference_id: GO_REF:0000107
  review:
    summary: negative regulation of protein-containing complex assembly pathway context
    action: KEEP_AS_NON_CORE
    reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather
      than SRC core molecular activity.
- term:
    id: GO:0034139
    label: regulation of toll-like receptor 3 signaling pathway
  evidence_type: IEA
  original_reference_id: GO_REF:0000107
  review:
    summary: regulation of toll-like receptor 3 signaling pathway pathway context
    action: KEEP_AS_NON_CORE
    reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather
      than SRC core molecular activity.
- term:
    id: GO:0034614
    label: cellular response to reactive oxygen species
  evidence_type: IEA
  original_reference_id: GO_REF:0000107
  review:
    summary: cellular response to reactive oxygen species pathway context
    action: KEEP_AS_NON_CORE
    reason: This term captures a stimulus or pathway context in which SRC can act, not the core SRC molecular
      activity.
- term:
    id: GO:0035306
    label: positive regulation of dephosphorylation
  evidence_type: IEA
  original_reference_id: GO_REF:0000107
  review:
    summary: positive regulation of dephosphorylation pathway context
    action: KEEP_AS_NON_CORE
    reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather
      than SRC core molecular activity.
- term:
    id: GO:0035331
    label: negative regulation of hippo signaling
  evidence_type: IEA
  original_reference_id: GO_REF:0000107
  review:
    summary: negative regulation of hippo signaling pathway context
    action: KEEP_AS_NON_CORE
    reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather
      than SRC core molecular activity.
- term:
    id: GO:0035556
    label: intracellular signal transduction
  evidence_type: IEA
  original_reference_id: GO_REF:0000107
  review:
    summary: SRC intracellular signaling function
    action: ACCEPT
    reason: SRC acts near activated receptors and adhesion complexes to propagate tyrosine-phosphorylation-dependent
      intracellular signaling.
- term:
    id: GO:0042169
    label: SH2 domain binding
  evidence_type: IEA
  original_reference_id: GO_REF:0000107
  review:
    summary: SH2 domain binding supports SRC recruitment or substrate recognition
    action: KEEP_AS_NON_CORE
    reason: This interaction is mechanistically relevant to SRC signaling but is accessory to the core
      tyrosine kinase activity.
- term:
    id: GO:0043066
    label: negative regulation of apoptotic process
  evidence_type: IEA
  original_reference_id: GO_REF:0000107
  review:
    summary: negative regulation of apoptotic process pathway context
    action: KEEP_AS_NON_CORE
    reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather
      than SRC core molecular activity.
- term:
    id: GO:0043149
    label: stress fiber assembly
  evidence_type: IEA
  original_reference_id: GO_REF:0000107
  review:
    summary: stress fiber assembly pathway context
    action: KEEP_AS_NON_CORE
    reason: The annotation is compatible with SRC pathway biology but represents a context-specific process,
      location, or phenotype rather than the core kinase function.
- term:
    id: GO:0043274
    label: phospholipase binding
  evidence_type: IEA
  original_reference_id: GO_REF:0000107
  review:
    summary: phospholipase binding pathway context
    action: KEEP_AS_NON_CORE
    reason: The annotation is compatible with SRC pathway biology but represents a context-specific process,
      location, or phenotype rather than the core kinase function.
- term:
    id: GO:0044325
    label: transmembrane transporter binding
  evidence_type: IEA
  original_reference_id: GO_REF:0000107
  review:
    summary: transmembrane transporter binding pathway context
    action: KEEP_AS_NON_CORE
    reason: The annotation is compatible with SRC pathway biology but represents a context-specific process,
      location, or phenotype rather than the core kinase function.
- term:
    id: GO:0045747
    label: positive regulation of Notch signaling pathway
  evidence_type: IEA
  original_reference_id: GO_REF:0000107
  review:
    summary: positive regulation of Notch signaling pathway pathway context
    action: KEEP_AS_NON_CORE
    reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather
      than SRC core molecular activity.
- term:
    id: GO:0046875
    label: ephrin receptor binding
  evidence_type: IEA
  original_reference_id: GO_REF:0000107
  review:
    summary: ephrin receptor binding supports SRC recruitment or substrate recognition
    action: KEEP_AS_NON_CORE
    reason: This interaction is mechanistically relevant to SRC signaling but is accessory to the core
      tyrosine kinase activity.
- term:
    id: GO:0048041
    label: focal adhesion assembly
  evidence_type: IEA
  original_reference_id: GO_REF:0000107
  review:
    summary: focal adhesion assembly pathway context
    action: KEEP_AS_NON_CORE
    reason: The annotation is compatible with SRC pathway biology but represents a context-specific process,
      location, or phenotype rather than the core kinase function.
- term:
    id: GO:0051057
    label: positive regulation of small GTPase mediated signal transduction
  evidence_type: IEA
  original_reference_id: GO_REF:0000120
  review:
    summary: positive regulation of small GTPase mediated signal transduction pathway context
    action: KEEP_AS_NON_CORE
    reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather
      than SRC core molecular activity.
- term:
    id: GO:0051219
    label: phosphoprotein binding
  evidence_type: IEA
  original_reference_id: GO_REF:0000107
  review:
    summary: phosphoprotein binding supports SRC recruitment or substrate recognition
    action: KEEP_AS_NON_CORE
    reason: This interaction is mechanistically relevant to SRC signaling but is accessory to the core
      tyrosine kinase activity.
- term:
    id: GO:0051897
    label: positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction
  evidence_type: IEA
  original_reference_id: GO_REF:0000107
  review:
    summary: positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction
      pathway context
    action: KEEP_AS_NON_CORE
    reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather
      than SRC core molecular activity.
- term:
    id: GO:0051902
    label: negative regulation of mitochondrial depolarization
  evidence_type: IEA
  original_reference_id: GO_REF:0000107
  review:
    summary: negative regulation of mitochondrial depolarization pathway context
    action: KEEP_AS_NON_CORE
    reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather
      than SRC core molecular activity.
- term:
    id: GO:0060576
    label: intestinal epithelial cell development
  evidence_type: IEA
  original_reference_id: GO_REF:0000107
  review:
    summary: intestinal epithelial cell development pathway context
    action: KEEP_AS_NON_CORE
    reason: The annotation is compatible with SRC pathway biology but represents a context-specific process,
      location, or phenotype rather than the core kinase function.
- term:
    id: GO:0070102
    label: interleukin-6-mediated signaling pathway
  evidence_type: IEA
  original_reference_id: GO_REF:0000107
  review:
    summary: interleukin-6-mediated signaling pathway pathway context
    action: KEEP_AS_NON_CORE
    reason: The annotation is compatible with SRC pathway biology but represents a context-specific process,
      location, or phenotype rather than the core kinase function.
- term:
    id: GO:0070555
    label: response to interleukin-1
  evidence_type: IEA
  original_reference_id: GO_REF:0000107
  review:
    summary: response to interleukin-1 pathway context
    action: KEEP_AS_NON_CORE
    reason: This term captures a stimulus or pathway context in which SRC can act, not the core SRC molecular
      activity.
- term:
    id: GO:0070700
    label: BMP receptor binding
  evidence_type: IEA
  original_reference_id: GO_REF:0000107
  review:
    summary: BMP receptor binding pathway context
    action: KEEP_AS_NON_CORE
    reason: The annotation is compatible with SRC pathway biology but represents a context-specific process,
      location, or phenotype rather than the core kinase function.
- term:
    id: GO:0097110
    label: scaffold protein binding
  evidence_type: IEA
  original_reference_id: GO_REF:0000107
  review:
    summary: scaffold protein binding supports SRC recruitment or substrate recognition
    action: KEEP_AS_NON_CORE
    reason: This interaction is mechanistically relevant to SRC signaling but is accessory to the core
      tyrosine kinase activity.
- term:
    id: GO:1902564
    label: negative regulation of neutrophil activation
  evidence_type: IEA
  original_reference_id: GO_REF:0000107
  review:
    summary: negative regulation of neutrophil activation pathway context
    action: KEEP_AS_NON_CORE
    reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather
      than SRC core molecular activity.
- term:
    id: GO:1904263
    label: positive regulation of TORC1 signaling
  evidence_type: IEA
  original_reference_id: GO_REF:0000107
  review:
    summary: positive regulation of TORC1 signaling pathway context
    action: KEEP_AS_NON_CORE
    reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather
      than SRC core molecular activity.
- term:
    id: GO:2000394
    label: positive regulation of lamellipodium morphogenesis
  evidence_type: IEA
  original_reference_id: GO_REF:0000107
  review:
    summary: positive regulation of lamellipodium morphogenesis pathway context
    action: KEEP_AS_NON_CORE
    reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather
      than SRC core molecular activity.
- term:
    id: GO:2000641
    label: regulation of early endosome to late endosome transport
  evidence_type: IEA
  original_reference_id: GO_REF:0000107
  review:
    summary: regulation of early endosome to late endosome transport pathway context
    action: KEEP_AS_NON_CORE
    reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather
      than SRC core molecular activity.
- term:
    id: GO:2000811
    label: negative regulation of anoikis
  evidence_type: IEA
  original_reference_id: GO_REF:0000107
  review:
    summary: negative regulation of anoikis pathway context
    action: KEEP_AS_NON_CORE
    reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather
      than SRC core molecular activity.
- term:
    id: GO:2001237
    label: negative regulation of extrinsic apoptotic signaling pathway
  evidence_type: IEA
  original_reference_id: GO_REF:0000107
  review:
    summary: negative regulation of extrinsic apoptotic signaling pathway pathway context
    action: KEEP_AS_NON_CORE
    reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather
      than SRC core molecular activity.
- term:
    id: GO:2001243
    label: negative regulation of intrinsic apoptotic signaling pathway
  evidence_type: IEA
  original_reference_id: GO_REF:0000107
  review:
    summary: negative regulation of intrinsic apoptotic signaling pathway pathway context
    action: KEEP_AS_NON_CORE
    reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather
      than SRC core molecular activity.
- term:
    id: GO:2001286
    label: regulation of caveolin-mediated endocytosis
  evidence_type: IEA
  original_reference_id: GO_REF:0000107
  review:
    summary: regulation of caveolin-mediated endocytosis pathway context
    action: KEEP_AS_NON_CORE
    reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather
      than SRC core molecular activity.
- term:
    id: GO:0000122
    label: negative regulation of transcription by RNA polymerase II
  evidence_type: ISO
  original_reference_id: GO_REF:0000119
  review:
    summary: negative regulation of transcription by RNA polymerase II pathway context
    action: KEEP_AS_NON_CORE
    reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather
      than SRC core molecular activity.
- term:
    id: GO:0001545
    label: primary ovarian follicle growth
  evidence_type: ISO
  original_reference_id: GO_REF:0000096
  review:
    summary: primary ovarian follicle growth pathway context
    action: KEEP_AS_NON_CORE
    reason: The annotation is compatible with SRC pathway biology but represents a context-specific process,
      location, or phenotype rather than the core kinase function.
- term:
    id: GO:0001819
    label: positive regulation of cytokine production
  evidence_type: ISO
  original_reference_id: GO_REF:0000096
  review:
    summary: positive regulation of cytokine production pathway context
    action: KEEP_AS_NON_CORE
    reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather
      than SRC core molecular activity.
- term:
    id: GO:0004672
    label: protein kinase activity
  evidence_type: ISO
  original_reference_id: GO_REF:0000119
  review:
    summary: protein kinase activity is less specific than SRC tyrosine kinase activity
    action: MARK_AS_OVER_ANNOTATED
    reason: The annotation is directionally correct but over-broad because more specific SRC tyrosine
      kinase terms are present.
- term:
    id: GO:0004713
    label: protein tyrosine kinase activity
  evidence_type: ISO
  original_reference_id: GO_REF:0000119
  review:
    summary: Core SRC non-receptor tyrosine kinase activity
    action: ACCEPT
    reason: SRC is a non-receptor tyrosine kinase; tyrosine phosphorylation of protein substrates is its
      primary molecular activity.
- term:
    id: GO:0004715
    label: non-membrane spanning protein tyrosine kinase activity
  evidence_type: ISO
  original_reference_id: GO_REF:0000119
  review:
    summary: Core SRC non-receptor tyrosine kinase activity
    action: ACCEPT
    reason: SRC is a non-receptor tyrosine kinase; tyrosine phosphorylation of protein substrates is its
      primary molecular activity.
    supported_by:
    - reference_id: file:mouse/Src/Src-deep-research-bioreason.md
      supporting_text: A cytoplasmic non-receptor tyrosine kinase that uses an SH3-SH2-kinase architecture
        to assemble with proline-rich and phosphotyrosine-containing partners
- term:
    id: GO:0005102
    label: signaling receptor binding
  evidence_type: ISO
  original_reference_id: GO_REF:0000119
  review:
    summary: signaling receptor binding supports SRC recruitment or substrate recognition
    action: KEEP_AS_NON_CORE
    reason: This interaction is mechanistically relevant to SRC signaling but is accessory to the core
      tyrosine kinase activity.
- term:
    id: GO:0005178
    label: integrin binding
  evidence_type: ISO
  original_reference_id: GO_REF:0000119
  review:
    summary: integrin binding pathway context
    action: KEEP_AS_NON_CORE
    reason: The annotation is compatible with SRC pathway biology but represents a context-specific process,
      location, or phenotype rather than the core kinase function.
- term:
    id: GO:0005737
    label: cytoplasm
  evidence_type: ISO
  original_reference_id: GO_REF:0000119
  review:
    summary: cytoplasm localization
    action: ACCEPT
    reason: SRC is a soluble non-receptor kinase recruited to cytosolic, membrane, junctional, caveolar,
      and focal-adhesion signaling assemblies.
- term:
    id: GO:0005739
    label: mitochondrion
  evidence_type: ISO
  original_reference_id: GO_REF:0000119
  review:
    summary: mitochondrion pathway context
    action: KEEP_AS_NON_CORE
    reason: The annotation is compatible with SRC pathway biology but represents a context-specific process,
      location, or phenotype rather than the core kinase function.
- term:
    id: GO:0005743
    label: mitochondrial inner membrane
  evidence_type: ISO
  original_reference_id: GO_REF:0000119
  review:
    summary: mitochondrial inner membrane pathway context
    action: KEEP_AS_NON_CORE
    reason: The annotation is compatible with SRC pathway biology but represents a context-specific process,
      location, or phenotype rather than the core kinase function.
- term:
    id: GO:0005764
    label: lysosome
  evidence_type: ISO
  original_reference_id: GO_REF:0000119
  review:
    summary: lysosome pathway context
    action: KEEP_AS_NON_CORE
    reason: The annotation is compatible with SRC pathway biology but represents a context-specific process,
      location, or phenotype rather than the core kinase function.
- term:
    id: GO:0005770
    label: late endosome
  evidence_type: ISO
  original_reference_id: GO_REF:0000119
  review:
    summary: late endosome pathway context
    action: KEEP_AS_NON_CORE
    reason: The annotation is compatible with SRC pathway biology but represents a context-specific process,
      location, or phenotype rather than the core kinase function.
- term:
    id: GO:0005829
    label: cytosol
  evidence_type: ISO
  original_reference_id: GO_REF:0000119
  review:
    summary: cytosol localization
    action: ACCEPT
    reason: SRC is a soluble non-receptor kinase recruited to cytosolic, membrane, junctional, caveolar,
      and focal-adhesion signaling assemblies.
- term:
    id: GO:0005886
    label: plasma membrane
  evidence_type: ISO
  original_reference_id: GO_REF:0000119
  review:
    summary: plasma membrane localization
    action: ACCEPT
    reason: SRC is a soluble non-receptor kinase recruited to cytosolic, membrane, junctional, caveolar,
      and focal-adhesion signaling assemblies.
- term:
    id: GO:0005901
    label: caveola
  evidence_type: ISO
  original_reference_id: GO_REF:0000119
  review:
    summary: caveola localization
    action: ACCEPT
    reason: SRC is a soluble non-receptor kinase recruited to cytosolic, membrane, junctional, caveolar,
      and focal-adhesion signaling assemblies.
- term:
    id: GO:0005911
    label: cell-cell junction
  evidence_type: ISO
  original_reference_id: GO_REF:0000119
  review:
    summary: cell-cell junction localization
    action: ACCEPT
    reason: SRC is a soluble non-receptor kinase recruited to cytosolic, membrane, junctional, caveolar,
      and focal-adhesion signaling assemblies.
- term:
    id: GO:0007179
    label: transforming growth factor beta receptor signaling pathway
  evidence_type: ISO
  original_reference_id: GO_REF:0000119
  review:
    summary: transforming growth factor beta receptor signaling pathway pathway context
    action: KEEP_AS_NON_CORE
    reason: The annotation is compatible with SRC pathway biology but represents a context-specific process,
      location, or phenotype rather than the core kinase function.
- term:
    id: GO:0007229
    label: integrin-mediated signaling pathway
  evidence_type: ISO
  original_reference_id: GO_REF:0000119
  review:
    summary: integrin-mediated signaling pathway pathway context
    action: KEEP_AS_NON_CORE
    reason: The annotation is compatible with SRC pathway biology but represents a context-specific process,
      location, or phenotype rather than the core kinase function.
- term:
    id: GO:0008284
    label: positive regulation of cell population proliferation
  evidence_type: ISO
  original_reference_id: GO_REF:0000096
  review:
    summary: positive regulation of cell population proliferation pathway context
    action: KEEP_AS_NON_CORE
    reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather
      than SRC core molecular activity.
- term:
    id: GO:0010628
    label: positive regulation of gene expression
  evidence_type: ISO
  original_reference_id: GO_REF:0000096
  review:
    summary: positive regulation of gene expression pathway context
    action: KEEP_AS_NON_CORE
    reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather
      than SRC core molecular activity.
- term:
    id: GO:0010629
    label: negative regulation of gene expression
  evidence_type: ISO
  original_reference_id: GO_REF:0000096
  review:
    summary: negative regulation of gene expression pathway context
    action: KEEP_AS_NON_CORE
    reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather
      than SRC core molecular activity.
- term:
    id: GO:0010632
    label: regulation of epithelial cell migration
  evidence_type: ISO
  original_reference_id: GO_REF:0000119
  review:
    summary: regulation of epithelial cell migration pathway context
    action: KEEP_AS_NON_CORE
    reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather
      than SRC core molecular activity.
- term:
    id: GO:0010634
    label: positive regulation of epithelial cell migration
  evidence_type: ISO
  original_reference_id: GO_REF:0000119
  review:
    summary: positive regulation of epithelial cell migration pathway context
    action: KEEP_AS_NON_CORE
    reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather
      than SRC core molecular activity.
- term:
    id: GO:0010907
    label: positive regulation of glucose metabolic process
  evidence_type: ISO
  original_reference_id: GO_REF:0000096
  review:
    summary: positive regulation of glucose metabolic process pathway context
    action: KEEP_AS_NON_CORE
    reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather
      than SRC core molecular activity.
- term:
    id: GO:0014911
    label: positive regulation of smooth muscle cell migration
  evidence_type: ISO
  original_reference_id: GO_REF:0000096
  review:
    summary: positive regulation of smooth muscle cell migration pathway context
    action: KEEP_AS_NON_CORE
    reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather
      than SRC core molecular activity.
- term:
    id: GO:0016004
    label: phospholipase activator activity
  evidence_type: ISO
  original_reference_id: GO_REF:0000119
  review:
    summary: phospholipase activator activity pathway context
    action: KEEP_AS_NON_CORE
    reason: The annotation is compatible with SRC pathway biology but represents a context-specific process,
      location, or phenotype rather than the core kinase function.
- term:
    id: GO:0019899
    label: enzyme binding
  evidence_type: ISO
  original_reference_id: GO_REF:0000119
  review:
    summary: Generic enzyme binding annotation
    action: MARK_AS_OVER_ANNOTATED
    reason: The term is too broad or peripheral to capture SRC function when more specific tyrosine kinase
      and signaling annotations are available.
- term:
    id: GO:0020037
    label: heme binding
  evidence_type: ISO
  original_reference_id: GO_REF:0000119
  review:
    summary: Generic heme binding annotation
    action: MARK_AS_OVER_ANNOTATED
    reason: The term is too broad or peripheral to capture SRC function when more specific tyrosine kinase
      and signaling annotations are available.
- term:
    id: GO:0022407
    label: regulation of cell-cell adhesion
  evidence_type: ISO
  original_reference_id: GO_REF:0000119
  review:
    summary: regulation of cell-cell adhesion pathway context
    action: KEEP_AS_NON_CORE
    reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather
      than SRC core molecular activity.
- term:
    id: GO:0030054
    label: cell junction
  evidence_type: ISO
  original_reference_id: GO_REF:0000119
  review:
    summary: cell junction localization
    action: ACCEPT
    reason: SRC is a soluble non-receptor kinase recruited to cytosolic, membrane, junctional, caveolar,
      and focal-adhesion signaling assemblies.
- term:
    id: GO:0030335
    label: positive regulation of cell migration
  evidence_type: ISO
  original_reference_id: GO_REF:0000096
  review:
    summary: positive regulation of cell migration pathway context
    action: KEEP_AS_NON_CORE
    reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather
      than SRC core molecular activity.
- term:
    id: GO:0031333
    label: negative regulation of protein-containing complex assembly
  evidence_type: ISO
  original_reference_id: GO_REF:0000119
  review:
    summary: negative regulation of protein-containing complex assembly pathway context
    action: KEEP_AS_NON_CORE
    reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather
      than SRC core molecular activity.
- term:
    id: GO:0032205
    label: negative regulation of telomere maintenance
  evidence_type: ISO
  original_reference_id: GO_REF:0000119
  review:
    summary: negative regulation of telomere maintenance pathway context
    action: KEEP_AS_NON_CORE
    reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather
      than SRC core molecular activity.
- term:
    id: GO:0034139
    label: regulation of toll-like receptor 3 signaling pathway
  evidence_type: ISO
  original_reference_id: GO_REF:0000119
  review:
    summary: regulation of toll-like receptor 3 signaling pathway pathway context
    action: KEEP_AS_NON_CORE
    reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather
      than SRC core molecular activity.
- term:
    id: GO:0034614
    label: cellular response to reactive oxygen species
  evidence_type: ISO
  original_reference_id: GO_REF:0000119
  review:
    summary: cellular response to reactive oxygen species pathway context
    action: KEEP_AS_NON_CORE
    reason: This term captures a stimulus or pathway context in which SRC can act, not the core SRC molecular
      activity.
- term:
    id: GO:0035306
    label: positive regulation of dephosphorylation
  evidence_type: ISO
  original_reference_id: GO_REF:0000119
  review:
    summary: positive regulation of dephosphorylation pathway context
    action: KEEP_AS_NON_CORE
    reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather
      than SRC core molecular activity.
- term:
    id: GO:0035331
    label: negative regulation of hippo signaling
  evidence_type: ISO
  original_reference_id: GO_REF:0000119
  review:
    summary: negative regulation of hippo signaling pathway context
    action: KEEP_AS_NON_CORE
    reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather
      than SRC core molecular activity.
- term:
    id: GO:0035556
    label: intracellular signal transduction
  evidence_type: ISO
  original_reference_id: GO_REF:0000119
  review:
    summary: SRC intracellular signaling function
    action: ACCEPT
    reason: SRC acts near activated receptors and adhesion complexes to propagate tyrosine-phosphorylation-dependent
      intracellular signaling.
- term:
    id: GO:0038083
    label: peptidyl-tyrosine autophosphorylation
  evidence_type: ISO
  original_reference_id: GO_REF:0000096
  review:
    summary: Direct SRC tyrosine phosphorylation activity
    action: ACCEPT
    reason: This term directly describes the catalytic output of SRC kinase activity on protein tyrosine
      residues.
- term:
    id: GO:0042169
    label: SH2 domain binding
  evidence_type: ISO
  original_reference_id: GO_REF:0000119
  review:
    summary: SH2 domain binding supports SRC recruitment or substrate recognition
    action: KEEP_AS_NON_CORE
    reason: This interaction is mechanistically relevant to SRC signaling but is accessory to the core
      tyrosine kinase activity.
- term:
    id: GO:0042311
    label: vasodilation
  evidence_type: ISO
  original_reference_id: GO_REF:0000119
  review:
    summary: vasodilation pathway context
    action: KEEP_AS_NON_CORE
    reason: The annotation is compatible with SRC pathway biology but represents a context-specific process,
      location, or phenotype rather than the core kinase function.
- term:
    id: GO:0043065
    label: positive regulation of apoptotic process
  evidence_type: ISO
  original_reference_id: GO_REF:0000096
  review:
    summary: positive regulation of apoptotic process pathway context
    action: KEEP_AS_NON_CORE
    reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather
      than SRC core molecular activity.
- term:
    id: GO:0043066
    label: negative regulation of apoptotic process
  evidence_type: ISO
  original_reference_id: GO_REF:0000119
  review:
    summary: negative regulation of apoptotic process pathway context
    action: KEEP_AS_NON_CORE
    reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather
      than SRC core molecular activity.
- term:
    id: GO:0043149
    label: stress fiber assembly
  evidence_type: ISO
  original_reference_id: GO_REF:0000119
  review:
    summary: stress fiber assembly pathway context
    action: KEEP_AS_NON_CORE
    reason: The annotation is compatible with SRC pathway biology but represents a context-specific process,
      location, or phenotype rather than the core kinase function.
- term:
    id: GO:0043274
    label: phospholipase binding
  evidence_type: ISO
  original_reference_id: GO_REF:0000119
  review:
    summary: phospholipase binding pathway context
    action: KEEP_AS_NON_CORE
    reason: The annotation is compatible with SRC pathway biology but represents a context-specific process,
      location, or phenotype rather than the core kinase function.
- term:
    id: GO:0043406
    label: positive regulation of MAP kinase activity
  evidence_type: ISO
  original_reference_id: GO_REF:0000096
  review:
    summary: positive regulation of MAP kinase activity pathway context
    action: KEEP_AS_NON_CORE
    reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather
      than SRC core molecular activity.
- term:
    id: GO:0044325
    label: transmembrane transporter binding
  evidence_type: ISO
  original_reference_id: GO_REF:0000119
  review:
    summary: transmembrane transporter binding pathway context
    action: KEEP_AS_NON_CORE
    reason: The annotation is compatible with SRC pathway biology but represents a context-specific process,
      location, or phenotype rather than the core kinase function.
- term:
    id: GO:0045056
    label: transcytosis
  evidence_type: ISO
  original_reference_id: GO_REF:0000096
  review:
    summary: transcytosis pathway context
    action: KEEP_AS_NON_CORE
    reason: The annotation is compatible with SRC pathway biology but represents a context-specific process,
      location, or phenotype rather than the core kinase function.
- term:
    id: GO:0045747
    label: positive regulation of Notch signaling pathway
  evidence_type: ISO
  original_reference_id: GO_REF:0000119
  review:
    summary: positive regulation of Notch signaling pathway pathway context
    action: KEEP_AS_NON_CORE
    reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather
      than SRC core molecular activity.
- term:
    id: GO:0045780
    label: positive regulation of bone resorption
  evidence_type: ISO
  original_reference_id: GO_REF:0000096
  review:
    summary: positive regulation of bone resorption pathway context
    action: KEEP_AS_NON_CORE
    reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather
      than SRC core molecular activity.
- term:
    id: GO:0045785
    label: positive regulation of cell adhesion
  evidence_type: ISO
  original_reference_id: GO_REF:0000096
  review:
    summary: positive regulation of cell adhesion pathway context
    action: KEEP_AS_NON_CORE
    reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather
      than SRC core molecular activity.
- term:
    id: GO:0045793
    label: positive regulation of cell size
  evidence_type: ISO
  original_reference_id: GO_REF:0000096
  review:
    summary: positive regulation of cell size pathway context
    action: KEEP_AS_NON_CORE
    reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather
      than SRC core molecular activity.
- term:
    id: GO:0046579
    label: positive regulation of Ras protein signal transduction
  evidence_type: ISO
  original_reference_id: GO_REF:0000096
  review:
    summary: positive regulation of Ras protein signal transduction pathway context
    action: KEEP_AS_NON_CORE
    reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather
      than SRC core molecular activity.
- term:
    id: GO:0046628
    label: positive regulation of insulin receptor signaling pathway
  evidence_type: ISO
  original_reference_id: GO_REF:0000096
  review:
    summary: positive regulation of insulin receptor signaling pathway pathway context
    action: KEEP_AS_NON_CORE
    reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather
      than SRC core molecular activity.
- term:
    id: GO:0046875
    label: ephrin receptor binding
  evidence_type: ISO
  original_reference_id: GO_REF:0000119
  review:
    summary: ephrin receptor binding supports SRC recruitment or substrate recognition
    action: KEEP_AS_NON_CORE
    reason: This interaction is mechanistically relevant to SRC signaling but is accessory to the core
      tyrosine kinase activity.
- term:
    id: GO:0048011
    label: neurotrophin TRK receptor signaling pathway
  evidence_type: ISO
  original_reference_id: GO_REF:0000096
  review:
    summary: neurotrophin TRK receptor signaling pathway pathway context
    action: KEEP_AS_NON_CORE
    reason: The annotation is compatible with SRC pathway biology but represents a context-specific process,
      location, or phenotype rather than the core kinase function.
- term:
    id: GO:0048041
    label: focal adhesion assembly
  evidence_type: ISO
  original_reference_id: GO_REF:0000119
  review:
    summary: focal adhesion assembly pathway context
    action: KEEP_AS_NON_CORE
    reason: The annotation is compatible with SRC pathway biology but represents a context-specific process,
      location, or phenotype rather than the core kinase function.
- term:
    id: GO:0048471
    label: perinuclear region of cytoplasm
  evidence_type: ISO
  original_reference_id: GO_REF:0000119
  review:
    summary: perinuclear region of cytoplasm pathway context
    action: KEEP_AS_NON_CORE
    reason: The annotation is compatible with SRC pathway biology but represents a context-specific process,
      location, or phenotype rather than the core kinase function.
- term:
    id: GO:0050731
    label: positive regulation of peptidyl-tyrosine phosphorylation
  evidence_type: ISO
  original_reference_id: GO_REF:0000096
  review:
    summary: positive regulation of peptidyl-tyrosine phosphorylation pathway context
    action: KEEP_AS_NON_CORE
    reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather
      than SRC core molecular activity.
- term:
    id: GO:0050839
    label: cell adhesion molecule binding
  evidence_type: ISO
  original_reference_id: GO_REF:0000096
  review:
    summary: cell adhesion molecule binding pathway context
    action: KEEP_AS_NON_CORE
    reason: The annotation is compatible with SRC pathway biology but represents a context-specific process,
      location, or phenotype rather than the core kinase function.
- term:
    id: GO:0050847
    label: progesterone receptor signaling pathway
  evidence_type: ISO
  original_reference_id: GO_REF:0000096
  review:
    summary: progesterone receptor signaling pathway pathway context
    action: KEEP_AS_NON_CORE
    reason: The annotation is compatible with SRC pathway biology but represents a context-specific process,
      location, or phenotype rather than the core kinase function.
- term:
    id: GO:0051057
    label: positive regulation of small GTPase mediated signal transduction
  evidence_type: ISO
  original_reference_id: GO_REF:0000119
  review:
    summary: positive regulation of small GTPase mediated signal transduction pathway context
    action: KEEP_AS_NON_CORE
    reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather
      than SRC core molecular activity.
- term:
    id: GO:0051219
    label: phosphoprotein binding
  evidence_type: ISO
  original_reference_id: GO_REF:0000119
  review:
    summary: phosphoprotein binding supports SRC recruitment or substrate recognition
    action: KEEP_AS_NON_CORE
    reason: This interaction is mechanistically relevant to SRC signaling but is accessory to the core
      tyrosine kinase activity.
- term:
    id: GO:0051222
    label: positive regulation of protein transport
  evidence_type: ISO
  original_reference_id: GO_REF:0000096
  review:
    summary: positive regulation of protein transport pathway context
    action: KEEP_AS_NON_CORE
    reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather
      than SRC core molecular activity.
- term:
    id: GO:0051895
    label: negative regulation of focal adhesion assembly
  evidence_type: ISO
  original_reference_id: GO_REF:0000096
  review:
    summary: negative regulation of focal adhesion assembly pathway context
    action: KEEP_AS_NON_CORE
    reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather
      than SRC core molecular activity.
- term:
    id: GO:0051897
    label: positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction
  evidence_type: ISO
  original_reference_id: GO_REF:0000096
  review:
    summary: positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction
      pathway context
    action: KEEP_AS_NON_CORE
    reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather
      than SRC core molecular activity.
- term:
    id: GO:0051897
    label: positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction
  evidence_type: ISO
  original_reference_id: GO_REF:0000119
  review:
    summary: positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction
      pathway context
    action: KEEP_AS_NON_CORE
    reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather
      than SRC core molecular activity.
- term:
    id: GO:0051902
    label: negative regulation of mitochondrial depolarization
  evidence_type: ISO
  original_reference_id: GO_REF:0000119
  review:
    summary: negative regulation of mitochondrial depolarization pathway context
    action: KEEP_AS_NON_CORE
    reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather
      than SRC core molecular activity.
- term:
    id: GO:0060576
    label: intestinal epithelial cell development
  evidence_type: ISO
  original_reference_id: GO_REF:0000119
  review:
    summary: intestinal epithelial cell development pathway context
    action: KEEP_AS_NON_CORE
    reason: The annotation is compatible with SRC pathway biology but represents a context-specific process,
      location, or phenotype rather than the core kinase function.
- term:
    id: GO:0070102
    label: interleukin-6-mediated signaling pathway
  evidence_type: ISO
  original_reference_id: GO_REF:0000119
  review:
    summary: interleukin-6-mediated signaling pathway pathway context
    action: KEEP_AS_NON_CORE
    reason: The annotation is compatible with SRC pathway biology but represents a context-specific process,
      location, or phenotype rather than the core kinase function.
- term:
    id: GO:0070555
    label: response to interleukin-1
  evidence_type: ISO
  original_reference_id: GO_REF:0000119
  review:
    summary: response to interleukin-1 pathway context
    action: KEEP_AS_NON_CORE
    reason: This term captures a stimulus or pathway context in which SRC can act, not the core SRC molecular
      activity.
- term:
    id: GO:0070700
    label: BMP receptor binding
  evidence_type: ISO
  original_reference_id: GO_REF:0000119
  review:
    summary: BMP receptor binding pathway context
    action: KEEP_AS_NON_CORE
    reason: The annotation is compatible with SRC pathway biology but represents a context-specific process,
      location, or phenotype rather than the core kinase function.
- term:
    id: GO:0097110
    label: scaffold protein binding
  evidence_type: ISO
  original_reference_id: GO_REF:0000119
  review:
    summary: scaffold protein binding supports SRC recruitment or substrate recognition
    action: KEEP_AS_NON_CORE
    reason: This interaction is mechanistically relevant to SRC signaling but is accessory to the core
      tyrosine kinase activity.
- term:
    id: GO:1902564
    label: negative regulation of neutrophil activation
  evidence_type: ISO
  original_reference_id: GO_REF:0000119
  review:
    summary: negative regulation of neutrophil activation pathway context
    action: KEEP_AS_NON_CORE
    reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather
      than SRC core molecular activity.
- term:
    id: GO:1904263
    label: positive regulation of TORC1 signaling
  evidence_type: ISO
  original_reference_id: GO_REF:0000119
  review:
    summary: positive regulation of TORC1 signaling pathway context
    action: KEEP_AS_NON_CORE
    reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather
      than SRC core molecular activity.
- term:
    id: GO:2000394
    label: positive regulation of lamellipodium morphogenesis
  evidence_type: ISO
  original_reference_id: GO_REF:0000119
  review:
    summary: positive regulation of lamellipodium morphogenesis pathway context
    action: KEEP_AS_NON_CORE
    reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather
      than SRC core molecular activity.
- term:
    id: GO:2000641
    label: regulation of early endosome to late endosome transport
  evidence_type: ISO
  original_reference_id: GO_REF:0000119
  review:
    summary: regulation of early endosome to late endosome transport pathway context
    action: KEEP_AS_NON_CORE
    reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather
      than SRC core molecular activity.
- term:
    id: GO:2000811
    label: negative regulation of anoikis
  evidence_type: ISO
  original_reference_id: GO_REF:0000119
  review:
    summary: negative regulation of anoikis pathway context
    action: KEEP_AS_NON_CORE
    reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather
      than SRC core molecular activity.
- term:
    id: GO:2001237
    label: negative regulation of extrinsic apoptotic signaling pathway
  evidence_type: ISO
  original_reference_id: GO_REF:0000119
  review:
    summary: negative regulation of extrinsic apoptotic signaling pathway pathway context
    action: KEEP_AS_NON_CORE
    reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather
      than SRC core molecular activity.
- term:
    id: GO:2001243
    label: negative regulation of intrinsic apoptotic signaling pathway
  evidence_type: ISO
  original_reference_id: GO_REF:0000119
  review:
    summary: negative regulation of intrinsic apoptotic signaling pathway pathway context
    action: KEEP_AS_NON_CORE
    reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather
      than SRC core molecular activity.
- term:
    id: GO:2001286
    label: regulation of caveolin-mediated endocytosis
  evidence_type: ISO
  original_reference_id: GO_REF:0000119
  review:
    summary: regulation of caveolin-mediated endocytosis pathway context
    action: KEEP_AS_NON_CORE
    reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather
      than SRC core molecular activity.
- term:
    id: GO:0030331
    label: nuclear estrogen receptor binding
  evidence_type: ISO
  original_reference_id: GO_REF:0000096
  review:
    summary: nuclear estrogen receptor binding pathway context
    action: KEEP_AS_NON_CORE
    reason: The annotation is compatible with SRC pathway biology but represents a context-specific process,
      location, or phenotype rather than the core kinase function.
- term:
    id: GO:0030546
    label: signaling receptor activator activity
  evidence_type: ISO
  original_reference_id: GO_REF:0000096
  review:
    summary: signaling receptor activator activity pathway context
    action: KEEP_AS_NON_CORE
    reason: The annotation is compatible with SRC pathway biology but represents a context-specific process,
      location, or phenotype rather than the core kinase function.
- term:
    id: GO:0035255
    label: ionotropic glutamate receptor binding
  evidence_type: ISO
  original_reference_id: GO_REF:0000096
  review:
    summary: ionotropic glutamate receptor binding pathway context
    action: KEEP_AS_NON_CORE
    reason: The annotation is compatible with SRC pathway biology but represents a context-specific process,
      location, or phenotype rather than the core kinase function.
- term:
    id: GO:0044325
    label: transmembrane transporter binding
  evidence_type: ISO
  original_reference_id: GO_REF:0000096
  review:
    summary: transmembrane transporter binding pathway context
    action: KEEP_AS_NON_CORE
    reason: The annotation is compatible with SRC pathway biology but represents a context-specific process,
      location, or phenotype rather than the core kinase function.
- term:
    id: GO:0004713
    label: protein tyrosine kinase activity
  evidence_type: ISO
  original_reference_id: GO_REF:0000096
  review:
    summary: Core SRC non-receptor tyrosine kinase activity
    action: ACCEPT
    reason: SRC is a non-receptor tyrosine kinase; tyrosine phosphorylation of protein substrates is its
      primary molecular activity.
- term:
    id: GO:0005080
    label: protein kinase C binding
  evidence_type: ISO
  original_reference_id: GO_REF:0000096
  review:
    summary: protein kinase C binding pathway context
    action: KEEP_AS_NON_CORE
    reason: The annotation is compatible with SRC pathway biology but represents a context-specific process,
      location, or phenotype rather than the core kinase function.
- term:
    id: GO:0005102
    label: signaling receptor binding
  evidence_type: ISO
  original_reference_id: GO_REF:0000096
  review:
    summary: signaling receptor binding supports SRC recruitment or substrate recognition
    action: KEEP_AS_NON_CORE
    reason: This interaction is mechanistically relevant to SRC signaling but is accessory to the core
      tyrosine kinase activity.
- term:
    id: GO:0005158
    label: insulin receptor binding
  evidence_type: ISO
  original_reference_id: GO_REF:0000096
  review:
    summary: insulin receptor binding pathway context
    action: KEEP_AS_NON_CORE
    reason: The annotation is compatible with SRC pathway biology but represents a context-specific process,
      location, or phenotype rather than the core kinase function.
- term:
    id: GO:0005737
    label: cytoplasm
  evidence_type: ISO
  original_reference_id: GO_REF:0000096
  review:
    summary: cytoplasm localization
    action: ACCEPT
    reason: SRC is a soluble non-receptor kinase recruited to cytosolic, membrane, junctional, caveolar,
      and focal-adhesion signaling assemblies.
- term:
    id: GO:0005901
    label: caveola
  evidence_type: ISO
  original_reference_id: GO_REF:0000096
  review:
    summary: caveola localization
    action: ACCEPT
    reason: SRC is a soluble non-receptor kinase recruited to cytosolic, membrane, junctional, caveolar,
      and focal-adhesion signaling assemblies.
- term:
    id: GO:0016020
    label: membrane
  evidence_type: ISO
  original_reference_id: GO_REF:0000096
  review:
    summary: Generic membrane annotation
    action: MARK_AS_OVER_ANNOTATED
    reason: The term is too broad or peripheral to capture SRC function when more specific tyrosine kinase
      and signaling annotations are available.
- term:
    id: GO:0016579
    label: protein deubiquitination
  evidence_type: ISO
  original_reference_id: GO_REF:0000119
  review:
    summary: protein deubiquitination pathway context
    action: KEEP_AS_NON_CORE
    reason: The annotation is compatible with SRC pathway biology but represents a context-specific process,
      location, or phenotype rather than the core kinase function.
- term:
    id: GO:0019899
    label: enzyme binding
  evidence_type: ISO
  original_reference_id: GO_REF:0000096
  review:
    summary: Generic enzyme binding annotation
    action: MARK_AS_OVER_ANNOTATED
    reason: The term is too broad or peripheral to capture SRC function when more specific tyrosine kinase
      and signaling annotations are available.
- term:
    id: GO:0019901
    label: protein kinase binding
  evidence_type: ISO
  original_reference_id: GO_REF:0000096
  review:
    summary: Generic protein kinase binding annotation
    action: MARK_AS_OVER_ANNOTATED
    reason: The term is too broad or peripheral to capture SRC function when more specific tyrosine kinase
      and signaling annotations are available.
- term:
    id: GO:0030424
    label: axon
  evidence_type: ISO
  original_reference_id: GO_REF:0000096
  review:
    summary: axon pathway context
    action: KEEP_AS_NON_CORE
    reason: The annotation is compatible with SRC pathway biology but represents a context-specific process,
      location, or phenotype rather than the core kinase function.
- term:
    id: GO:0031669
    label: cellular response to nutrient levels
  evidence_type: ISO
  original_reference_id: GO_REF:0000119
  review:
    summary: cellular response to nutrient levels pathway context
    action: KEEP_AS_NON_CORE
    reason: This term captures a stimulus or pathway context in which SRC can act, not the core SRC molecular
      activity.
- term:
    id: GO:0034138
    label: toll-like receptor 3 signaling pathway
  evidence_type: ISO
  original_reference_id: GO_REF:0000119
  review:
    summary: toll-like receptor 3 signaling pathway pathway context
    action: KEEP_AS_NON_CORE
    reason: The annotation is compatible with SRC pathway biology but represents a context-specific process,
      location, or phenotype rather than the core kinase function.
- term:
    id: GO:0038202
    label: TORC1 signaling
  evidence_type: ISO
  original_reference_id: GO_REF:0000119
  review:
    summary: TORC1 signaling pathway context
    action: KEEP_AS_NON_CORE
    reason: The annotation is compatible with SRC pathway biology but represents a context-specific process,
      location, or phenotype rather than the core kinase function.
- term:
    id: GO:0043025
    label: neuronal cell body
  evidence_type: ISO
  original_reference_id: GO_REF:0000096
  review:
    summary: neuronal cell body pathway context
    action: KEEP_AS_NON_CORE
    reason: The annotation is compatible with SRC pathway biology but represents a context-specific process,
      location, or phenotype rather than the core kinase function.
- term:
    id: GO:0043161
    label: proteasome-mediated ubiquitin-dependent protein catabolic process
  evidence_type: ISO
  original_reference_id: GO_REF:0000119
  review:
    summary: proteasome-mediated ubiquitin-dependent protein catabolic process pathway context
    action: KEEP_AS_NON_CORE
    reason: The annotation is compatible with SRC pathway biology but represents a context-specific process,
      location, or phenotype rather than the core kinase function.
- term:
    id: GO:0044294
    label: dendritic growth cone
  evidence_type: ISO
  original_reference_id: GO_REF:0000096
  review:
    summary: dendritic growth cone pathway context
    action: KEEP_AS_NON_CORE
    reason: The annotation is compatible with SRC pathway biology but represents a context-specific process,
      location, or phenotype rather than the core kinase function.
- term:
    id: GO:0044877
    label: protein-containing complex binding
  evidence_type: ISO
  original_reference_id: GO_REF:0000096
  review:
    summary: Generic protein-containing complex binding annotation
    action: MARK_AS_OVER_ANNOTATED
    reason: The term is too broad or peripheral to capture SRC function when more specific tyrosine kinase
      and signaling annotations are available.
- term:
    id: GO:0070374
    label: positive regulation of ERK1 and ERK2 cascade
  evidence_type: ISO
  original_reference_id: GO_REF:0000096
  review:
    summary: positive regulation of ERK1 and ERK2 cascade pathway context
    action: KEEP_AS_NON_CORE
    reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather
      than SRC core molecular activity.
- term:
    id: GO:0071393
    label: cellular response to progesterone stimulus
  evidence_type: ISO
  original_reference_id: GO_REF:0000096
  review:
    summary: cellular response to progesterone stimulus pathway context
    action: KEEP_AS_NON_CORE
    reason: This term captures a stimulus or pathway context in which SRC can act, not the core SRC molecular
      activity.
- term:
    id: GO:0071897
    label: DNA biosynthetic process
  evidence_type: ISO
  original_reference_id: GO_REF:0000096
  review:
    summary: DNA biosynthetic process pathway context
    action: KEEP_AS_NON_CORE
    reason: The annotation is compatible with SRC pathway biology but represents a context-specific process,
      location, or phenotype rather than the core kinase function.
- term:
    id: GO:0090303
    label: positive regulation of wound healing
  evidence_type: ISO
  original_reference_id: GO_REF:0000096
  review:
    summary: positive regulation of wound healing pathway context
    action: KEEP_AS_NON_CORE
    reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather
      than SRC core molecular activity.
- term:
    id: GO:0097060
    label: synaptic membrane
  evidence_type: ISO
  original_reference_id: GO_REF:0000096
  review:
    summary: synaptic membrane pathway context
    action: KEEP_AS_NON_CORE
    reason: The annotation is compatible with SRC pathway biology but represents a context-specific process,
      location, or phenotype rather than the core kinase function.
- term:
    id: GO:0098978
    label: glutamatergic synapse
  evidence_type: ISO
  original_reference_id: GO_REF:0000096
  review:
    summary: glutamatergic synapse pathway context
    action: KEEP_AS_NON_CORE
    reason: The annotation is compatible with SRC pathway biology but represents a context-specific process,
      location, or phenotype rather than the core kinase function.
- term:
    id: GO:0098984
    label: neuron to neuron synapse
  evidence_type: ISO
  original_reference_id: GO_REF:0000096
  review:
    summary: neuron to neuron synapse pathway context
    action: KEEP_AS_NON_CORE
    reason: The annotation is compatible with SRC pathway biology but represents a context-specific process,
      location, or phenotype rather than the core kinase function.
- term:
    id: GO:0099073
    label: mitochondrion-derived vesicle
  evidence_type: ISO
  original_reference_id: GO_REF:0000096
  review:
    summary: mitochondrion-derived vesicle pathway context
    action: KEEP_AS_NON_CORE
    reason: The annotation is compatible with SRC pathway biology but represents a context-specific process,
      location, or phenotype rather than the core kinase function.
- term:
    id: GO:0099091
    label: postsynaptic specialization, intracellular component
  evidence_type: ISO
  original_reference_id: GO_REF:0000096
  review:
    summary: postsynaptic specialization, intracellular component pathway context
    action: KEEP_AS_NON_CORE
    reason: The annotation is compatible with SRC pathway biology but represents a context-specific process,
      location, or phenotype rather than the core kinase function.
- term:
    id: GO:1902533
    label: positive regulation of intracellular signal transduction
  evidence_type: ISO
  original_reference_id: GO_REF:0000096
  review:
    summary: positive regulation of intracellular signal transduction pathway context
    action: KEEP_AS_NON_CORE
    reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather
      than SRC core molecular activity.
- term:
    id: GO:1902737
    label: dendritic filopodium
  evidence_type: ISO
  original_reference_id: GO_REF:0000096
  review:
    summary: dendritic filopodium pathway context
    action: KEEP_AS_NON_CORE
    reason: The annotation is compatible with SRC pathway biology but represents a context-specific process,
      location, or phenotype rather than the core kinase function.
- term:
    id: GO:1904262
    label: negative regulation of TORC1 signaling
  evidence_type: ISO
  original_reference_id: GO_REF:0000119
  review:
    summary: negative regulation of TORC1 signaling pathway context
    action: KEEP_AS_NON_CORE
    reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather
      than SRC core molecular activity.
- term:
    id: GO:1904707
    label: positive regulation of vascular associated smooth muscle cell proliferation
  evidence_type: ISO
  original_reference_id: GO_REF:0000096
  review:
    summary: positive regulation of vascular associated smooth muscle cell proliferation pathway context
    action: KEEP_AS_NON_CORE
    reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather
      than SRC core molecular activity.
- term:
    id: GO:2000179
    label: positive regulation of neural precursor cell proliferation
  evidence_type: ISO
  original_reference_id: GO_REF:0000096
  review:
    summary: positive regulation of neural precursor cell proliferation pathway context
    action: KEEP_AS_NON_CORE
    reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather
      than SRC core molecular activity.
- term:
    id: GO:2000256
    label: positive regulation of male germ cell proliferation
  evidence_type: ISO
  original_reference_id: GO_REF:0000096
  review:
    summary: positive regulation of male germ cell proliferation pathway context
    action: KEEP_AS_NON_CORE
    reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather
      than SRC core molecular activity.
- term:
    id: GO:2000386
    label: positive regulation of ovarian follicle development
  evidence_type: ISO
  original_reference_id: GO_REF:0000096
  review:
    summary: positive regulation of ovarian follicle development pathway context
    action: KEEP_AS_NON_CORE
    reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather
      than SRC core molecular activity.
- term:
    id: GO:0004713
    label: protein tyrosine kinase activity
  evidence_type: IDA
  original_reference_id: PMID:18044967
  review:
    summary: Core SRC non-receptor tyrosine kinase activity
    action: ACCEPT
    reason: SRC is a non-receptor tyrosine kinase; tyrosine phosphorylation of protein substrates is its
      primary molecular activity.
- term:
    id: GO:0035022
    label: positive regulation of Rac protein signal transduction
  evidence_type: IMP
  original_reference_id: PMID:18044967
  review:
    summary: positive regulation of Rac protein signal transduction pathway context
    action: KEEP_AS_NON_CORE
    reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather
      than SRC core molecular activity.
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:25043309
  review:
    summary: Generic protein binding
    action: MARK_AS_OVER_ANNOTATED
    reason: Protein binding does not identify a specific SRC substrate-recognition mode or catalytic activity
      and is less informative than SH2/SH3/domain-specific terms.
- term:
    id: GO:0005886
    label: plasma membrane
  evidence_type: IDA
  original_reference_id: PMID:25043309
  review:
    summary: plasma membrane localization
    action: ACCEPT
    reason: SRC is a soluble non-receptor kinase recruited to cytosolic, membrane, junctional, caveolar,
      and focal-adhesion signaling assemblies.
    supported_by:
    - reference_id: PMID:25043309
      supporting_text: activating signals trigger the trafficking of cSrc to the plasma membrane and
        its concomitant activation
- term:
    id: GO:0007595
    label: lactation
  evidence_type: IDA
  original_reference_id: PMID:25043309
  review:
    summary: lactation pathway context
    action: KEEP_AS_NON_CORE
    reason: The annotation is compatible with SRC pathway biology but represents a context-specific process,
      location, or phenotype rather than the core kinase function.
- term:
    id: GO:0035556
    label: intracellular signal transduction
  evidence_type: IMP
  original_reference_id: PMID:12091389
  review:
    summary: SRC intracellular signaling function
    action: ACCEPT
    reason: SRC acts near activated receptors and adhesion complexes to propagate tyrosine-phosphorylation-dependent
      intracellular signaling.
- term:
    id: GO:0004713
    label: protein tyrosine kinase activity
  evidence_type: EXP
  original_reference_id: PMID:22759635
  review:
    summary: Core SRC non-receptor tyrosine kinase activity
    action: ACCEPT
    reason: SRC is a non-receptor tyrosine kinase; tyrosine phosphorylation of protein substrates is its
      primary molecular activity.
- term:
    id: GO:0004713
    label: protein tyrosine kinase activity
  evidence_type: TAS
  original_reference_id: Reactome:R-MMU-3928622
  review:
    summary: Core SRC non-receptor tyrosine kinase activity
    action: ACCEPT
    reason: SRC is a non-receptor tyrosine kinase; tyrosine phosphorylation of protein substrates is its
      primary molecular activity.
- term:
    id: GO:0004713
    label: protein tyrosine kinase activity
  evidence_type: TAS
  original_reference_id: Reactome:R-MMU-418870
  review:
    summary: Core SRC non-receptor tyrosine kinase activity
    action: ACCEPT
    reason: SRC is a non-receptor tyrosine kinase; tyrosine phosphorylation of protein substrates is its
      primary molecular activity.
- term:
    id: GO:0004713
    label: protein tyrosine kinase activity
  evidence_type: TAS
  original_reference_id: Reactome:R-MMU-420382
  review:
    summary: Core SRC non-receptor tyrosine kinase activity
    action: ACCEPT
    reason: SRC is a non-receptor tyrosine kinase; tyrosine phosphorylation of protein substrates is its
      primary molecular activity.
- term:
    id: GO:0004713
    label: protein tyrosine kinase activity
  evidence_type: TAS
  original_reference_id: Reactome:R-MMU-9011252
  review:
    summary: Core SRC non-receptor tyrosine kinase activity
    action: ACCEPT
    reason: SRC is a non-receptor tyrosine kinase; tyrosine phosphorylation of protein substrates is its
      primary molecular activity.
- term:
    id: GO:0004713
    label: protein tyrosine kinase activity
  evidence_type: TAS
  original_reference_id: Reactome:R-MMU-9763891
  review:
    summary: Core SRC non-receptor tyrosine kinase activity
    action: ACCEPT
    reason: SRC is a non-receptor tyrosine kinase; tyrosine phosphorylation of protein substrates is its
      primary molecular activity.
- term:
    id: GO:0004713
    label: protein tyrosine kinase activity
  evidence_type: TAS
  original_reference_id: Reactome:R-NUL-9726861
  review:
    summary: Core SRC non-receptor tyrosine kinase activity
    action: ACCEPT
    reason: SRC is a non-receptor tyrosine kinase; tyrosine phosphorylation of protein substrates is its
      primary molecular activity.
- term:
    id: GO:0005911
    label: cell-cell junction
  evidence_type: ISS
  original_reference_id: GO_REF:0000024
  review:
    summary: cell-cell junction localization
    action: ACCEPT
    reason: SRC is a soluble non-receptor kinase recruited to cytosolic, membrane, junctional, caveolar,
      and focal-adhesion signaling assemblies.
- term:
    id: GO:0038166
    label: angiotensin-activated signaling pathway
  evidence_type: IGI
  original_reference_id: PMID:25017399
  review:
    summary: angiotensin-activated signaling pathway pathway context
    action: KEEP_AS_NON_CORE
    reason: The annotation is compatible with SRC pathway biology but represents a context-specific process,
      location, or phenotype rather than the core kinase function.
- term:
    id: GO:0086091
    label: regulation of heart rate by cardiac conduction
  evidence_type: IGI
  original_reference_id: PMID:25017399
  review:
    summary: regulation of heart rate by cardiac conduction pathway context
    action: KEEP_AS_NON_CORE
    reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather
      than SRC core molecular activity.
- term:
    id: GO:0045821
    label: positive regulation of glycolytic process
  evidence_type: IDA
  original_reference_id: PMID:35263589
  review:
    summary: positive regulation of glycolytic process pathway context
    action: KEEP_AS_NON_CORE
    reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather
      than SRC core molecular activity.
- term:
    id: GO:0004713
    label: protein tyrosine kinase activity
  evidence_type: IMP
  original_reference_id: PMID:27505886
  review:
    summary: Core SRC non-receptor tyrosine kinase activity
    action: ACCEPT
    reason: SRC is a non-receptor tyrosine kinase; tyrosine phosphorylation of protein substrates is its
      primary molecular activity.
- term:
    id: GO:0018108
    label: peptidyl-tyrosine phosphorylation
  evidence_type: IMP
  original_reference_id: PMID:27505886
  review:
    summary: Direct SRC tyrosine phosphorylation activity
    action: ACCEPT
    reason: This term directly describes the catalytic output of SRC kinase activity on protein tyrosine
      residues.
- term:
    id: GO:0045747
    label: positive regulation of Notch signaling pathway
  evidence_type: IDA
  original_reference_id: PMID:25731159
  review:
    summary: positive regulation of Notch signaling pathway pathway context
    action: KEEP_AS_NON_CORE
    reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather
      than SRC core molecular activity.
- term:
    id: GO:0060576
    label: intestinal epithelial cell development
  evidence_type: IDA
  original_reference_id: PMID:25731159
  review:
    summary: intestinal epithelial cell development pathway context
    action: KEEP_AS_NON_CORE
    reason: The annotation is compatible with SRC pathway biology but represents a context-specific process,
      location, or phenotype rather than the core kinase function.
- term:
    id: GO:0070102
    label: interleukin-6-mediated signaling pathway
  evidence_type: IDA
  original_reference_id: PMID:25731159
  review:
    summary: interleukin-6-mediated signaling pathway pathway context
    action: KEEP_AS_NON_CORE
    reason: The annotation is compatible with SRC pathway biology but represents a context-specific process,
      location, or phenotype rather than the core kinase function.
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:11274221
  review:
    summary: Generic protein binding
    action: MARK_AS_OVER_ANNOTATED
    reason: Protein binding does not identify a specific SRC substrate-recognition mode or catalytic activity
      and is less informative than SH2/SH3/domain-specific terms.
- term:
    id: GO:0005743
    label: mitochondrial inner membrane
  evidence_type: IDA
  original_reference_id: PMID:12615910
  review:
    summary: mitochondrial inner membrane pathway context
    action: KEEP_AS_NON_CORE
    reason: The annotation is compatible with SRC pathway biology but represents a context-specific process,
      location, or phenotype rather than the core kinase function.
- term:
    id: GO:0030054
    label: cell junction
  evidence_type: ISS
  original_reference_id: GO_REF:0000024
  review:
    summary: cell junction localization
    action: ACCEPT
    reason: SRC is a soluble non-receptor kinase recruited to cytosolic, membrane, junctional, caveolar,
      and focal-adhesion signaling assemblies.
- term:
    id: GO:0005925
    label: focal adhesion
  evidence_type: IMP
  original_reference_id: PMID:22801373
  review:
    summary: focal adhesion localization
    action: ACCEPT
    reason: SRC is a soluble non-receptor kinase recruited to cytosolic, membrane, junctional, caveolar,
      and focal-adhesion signaling assemblies.
- term:
    id: GO:0004713
    label: protein tyrosine kinase activity
  evidence_type: IDA
  original_reference_id: PMID:8910389
  review:
    summary: Core SRC non-receptor tyrosine kinase activity
    action: ACCEPT
    reason: SRC is a non-receptor tyrosine kinase; tyrosine phosphorylation of protein substrates is its
      primary molecular activity.
- term:
    id: GO:0005634
    label: nucleus
  evidence_type: IDA
  original_reference_id: PMID:12615910
  review:
    summary: nucleus pathway context
    action: KEEP_AS_NON_CORE
    reason: The annotation is compatible with SRC pathway biology but represents a context-specific process,
      location, or phenotype rather than the core kinase function.
- term:
    id: GO:0005739
    label: mitochondrion
  evidence_type: IDA
  original_reference_id: PMID:12615910
  review:
    summary: mitochondrion pathway context
    action: KEEP_AS_NON_CORE
    reason: The annotation is compatible with SRC pathway biology but represents a context-specific process,
      location, or phenotype rather than the core kinase function.
- term:
    id: GO:0005856
    label: cytoskeleton
  evidence_type: IDA
  original_reference_id: PMID:12615910
  review:
    summary: cytoskeleton pathway context
    action: KEEP_AS_NON_CORE
    reason: The annotation is compatible with SRC pathway biology but represents a context-specific process,
      location, or phenotype rather than the core kinase function.
- term:
    id: GO:0005886
    label: plasma membrane
  evidence_type: IDA
  original_reference_id: PMID:12615910
  review:
    summary: plasma membrane localization
    action: ACCEPT
    reason: SRC is a soluble non-receptor kinase recruited to cytosolic, membrane, junctional, caveolar,
      and focal-adhesion signaling assemblies.
    supported_by:
    - reference_id: PMID:12615910
      supporting_text: c-Src as a plasma membrane-associated molecular effector of a variety of extracellular
        stimuli
- term:
    id: GO:0016020
    label: membrane
  evidence_type: IMP
  original_reference_id: PMID:22801373
  review:
    summary: Generic membrane annotation
    action: MARK_AS_OVER_ANNOTATED
    reason: The term is too broad or peripheral to capture SRC function when more specific tyrosine kinase
      and signaling annotations are available.
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:18691334
  review:
    summary: Generic protein binding
    action: MARK_AS_OVER_ANNOTATED
    reason: Protein binding does not identify a specific SRC substrate-recognition mode or catalytic activity
      and is less informative than SH2/SH3/domain-specific terms.
- term:
    id: GO:0045453
    label: bone resorption
  evidence_type: IMP
  original_reference_id: PMID:19448635
  review:
    summary: bone resorption pathway context
    action: KEEP_AS_NON_CORE
    reason: The annotation is compatible with SRC pathway biology but represents a context-specific process,
      location, or phenotype rather than the core kinase function.
- term:
    id: GO:0042476
    label: odontogenesis
  evidence_type: IMP
  original_reference_id: PMID:23174213
  review:
    summary: odontogenesis pathway context
    action: KEEP_AS_NON_CORE
    reason: The annotation is compatible with SRC pathway biology but represents a context-specific process,
      location, or phenotype rather than the core kinase function.
- term:
    id: GO:0070301
    label: cellular response to hydrogen peroxide
  evidence_type: IMP
  original_reference_id: PMID:23651497
  review:
    summary: cellular response to hydrogen peroxide pathway context
    action: KEEP_AS_NON_CORE
    reason: This term captures a stimulus or pathway context in which SRC can act, not the core SRC molecular
      activity.
- term:
    id: GO:2000588
    label: positive regulation of platelet-derived growth factor receptor-beta signaling pathway
  evidence_type: IMP
  original_reference_id: PMID:23651497
  review:
    summary: positive regulation of platelet-derived growth factor receptor-beta signaling pathway pathway
      context
    action: KEEP_AS_NON_CORE
    reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather
      than SRC core molecular activity.
- term:
    id: GO:0004672
    label: protein kinase activity
  evidence_type: IDA
  original_reference_id: PMID:27994061
  review:
    summary: protein kinase activity is less specific than SRC tyrosine kinase activity
    action: MARK_AS_OVER_ANNOTATED
    reason: The annotation is directionally correct but over-broad because more specific SRC tyrosine
      kinase terms are present.
- term:
    id: GO:0006468
    label: protein phosphorylation
  evidence_type: IDA
  original_reference_id: PMID:27994061
  review:
    summary: PMID:27994061 supports FAK phosphorylation of Beclin1, but the cached abstract does not
      directly support Src-mediated protein phosphorylation.
    action: UNDECIDED
    reason: Full text or a direct Src-specific passage would be needed before accepting this evidence-specific
      annotation.
- term:
    id: GO:0005886
    label: plasma membrane
  evidence_type: TAS
  original_reference_id: Reactome:R-MMU-9011252
  review:
    summary: plasma membrane localization
    action: ACCEPT
    reason: SRC is a soluble non-receptor kinase recruited to cytosolic, membrane, junctional, caveolar,
      and focal-adhesion signaling assemblies.
- term:
    id: GO:0005886
    label: plasma membrane
  evidence_type: TAS
  original_reference_id: Reactome:R-NUL-9726861
  review:
    summary: plasma membrane localization
    action: ACCEPT
    reason: SRC is a soluble non-receptor kinase recruited to cytosolic, membrane, junctional, caveolar,
      and focal-adhesion signaling assemblies.
- term:
    id: GO:0005829
    label: cytosol
  evidence_type: TAS
  original_reference_id: Reactome:R-NUL-9700655
  review:
    summary: cytosol localization
    action: ACCEPT
    reason: SRC is a soluble non-receptor kinase recruited to cytosolic, membrane, junctional, caveolar,
      and focal-adhesion signaling assemblies.
- term:
    id: GO:0005737
    label: cytoplasm
  evidence_type: IDA
  original_reference_id: PMID:14699011
  review:
    summary: cytoplasm localization
    action: ACCEPT
    reason: SRC is a soluble non-receptor kinase recruited to cytosolic, membrane, junctional, caveolar,
      and focal-adhesion signaling assemblies.
- term:
    id: GO:0071253
    label: connexin binding
  evidence_type: IPI
  original_reference_id: PMID:14699011
  review:
    summary: connexin binding pathway context
    action: KEEP_AS_NON_CORE
    reason: The annotation is compatible with SRC pathway biology but represents a context-specific process,
      location, or phenotype rather than the core kinase function.
- term:
    id: GO:0005654
    label: nucleoplasm
  evidence_type: TAS
  original_reference_id: Reactome:R-MMU-8937703
  review:
    summary: nucleoplasm pathway context
    action: KEEP_AS_NON_CORE
    reason: The annotation is compatible with SRC pathway biology but represents a context-specific process,
      location, or phenotype rather than the core kinase function.
- term:
    id: GO:0005654
    label: nucleoplasm
  evidence_type: TAS
  original_reference_id: Reactome:R-MMU-8937738
  review:
    summary: nucleoplasm pathway context
    action: KEEP_AS_NON_CORE
    reason: The annotation is compatible with SRC pathway biology but represents a context-specific process,
      location, or phenotype rather than the core kinase function.
- term:
    id: GO:0002102
    label: podosome
  evidence_type: IDA
  original_reference_id: PMID:17893324
  review:
    summary: podosome pathway context
    action: KEEP_AS_NON_CORE
    reason: The annotation is compatible with SRC pathway biology but represents a context-specific process,
      location, or phenotype rather than the core kinase function.
- term:
    id: GO:0010954
    label: positive regulation of protein processing
  evidence_type: IGI
  original_reference_id: PMID:17344476
  review:
    summary: positive regulation of protein processing pathway context
    action: KEEP_AS_NON_CORE
    reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather
      than SRC core molecular activity.
- term:
    id: GO:0050731
    label: positive regulation of peptidyl-tyrosine phosphorylation
  evidence_type: IGI
  original_reference_id: PMID:17344476
  review:
    summary: positive regulation of peptidyl-tyrosine phosphorylation pathway context
    action: KEEP_AS_NON_CORE
    reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather
      than SRC core molecular activity.
- term:
    id: GO:1900182
    label: positive regulation of protein localization to nucleus
  evidence_type: IGI
  original_reference_id: PMID:17344476
  review:
    summary: positive regulation of protein localization to nucleus pathway context
    action: KEEP_AS_NON_CORE
    reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather
      than SRC core molecular activity.
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:20847314
  review:
    summary: Generic protein binding
    action: MARK_AS_OVER_ANNOTATED
    reason: Protein binding does not identify a specific SRC substrate-recognition mode or catalytic activity
      and is less informative than SH2/SH3/domain-specific terms.
- term:
    id: GO:0071803
    label: positive regulation of podosome assembly
  evidence_type: IDA
  original_reference_id: PMID:24127566
  review:
    summary: positive regulation of podosome assembly pathway context
    action: KEEP_AS_NON_CORE
    reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather
      than SRC core molecular activity.
- term:
    id: GO:0004672
    label: protein kinase activity
  evidence_type: IDA
  original_reference_id: PMID:24190966
  review:
    summary: protein kinase activity is less specific than SRC tyrosine kinase activity
    action: MARK_AS_OVER_ANNOTATED
    reason: The annotation is directionally correct but over-broad because more specific SRC tyrosine
      kinase terms are present.
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:24190966
  review:
    summary: Generic protein binding
    action: MARK_AS_OVER_ANNOTATED
    reason: Protein binding does not identify a specific SRC substrate-recognition mode or catalytic activity
      and is less informative than SH2/SH3/domain-specific terms.
- term:
    id: GO:0034614
    label: cellular response to reactive oxygen species
  evidence_type: IDA
  original_reference_id: PMID:24190966
  review:
    summary: cellular response to reactive oxygen species pathway context
    action: KEEP_AS_NON_CORE
    reason: This term captures a stimulus or pathway context in which SRC can act, not the core SRC molecular
      activity.
- term:
    id: GO:0005634
    label: nucleus
  evidence_type: IDA
  original_reference_id: PMID:24841674
  review:
    summary: nucleus pathway context
    action: KEEP_AS_NON_CORE
    reason: The annotation is compatible with SRC pathway biology but represents a context-specific process,
      location, or phenotype rather than the core kinase function.
- term:
    id: GO:0048471
    label: perinuclear region of cytoplasm
  evidence_type: IDA
  original_reference_id: PMID:24841674
  review:
    summary: perinuclear region of cytoplasm pathway context
    action: KEEP_AS_NON_CORE
    reason: The annotation is compatible with SRC pathway biology but represents a context-specific process,
      location, or phenotype rather than the core kinase function.
- term:
    id: GO:0071498
    label: cellular response to fluid shear stress
  evidence_type: IMP
  original_reference_id: PMID:24841674
  review:
    summary: cellular response to fluid shear stress pathway context
    action: KEEP_AS_NON_CORE
    reason: This term captures a stimulus or pathway context in which SRC can act, not the core SRC molecular
      activity.
- term:
    id: GO:0004713
    label: protein tyrosine kinase activity
  evidence_type: IMP
  original_reference_id: PMID:12091389
  review:
    summary: Core SRC non-receptor tyrosine kinase activity
    action: ACCEPT
    reason: SRC is a non-receptor tyrosine kinase; tyrosine phosphorylation of protein substrates is its
      primary molecular activity.
- term:
    id: GO:0005901
    label: caveola
  evidence_type: IDA
  original_reference_id: PMID:12091389
  review:
    summary: caveola localization
    action: ACCEPT
    reason: SRC is a soluble non-receptor kinase recruited to cytosolic, membrane, junctional, caveolar,
      and focal-adhesion signaling assemblies.
- term:
    id: GO:0097110
    label: scaffold protein binding
  evidence_type: IPI
  original_reference_id: PMID:12091389
  review:
    summary: scaffold protein binding supports SRC recruitment or substrate recognition
    action: KEEP_AS_NON_CORE
    reason: This interaction is mechanistically relevant to SRC signaling but is accessory to the core
      tyrosine kinase activity.
- term:
    id: GO:0031648
    label: protein destabilization
  evidence_type: IMP
  original_reference_id: PMID:25259869
  review:
    summary: protein destabilization pathway context
    action: KEEP_AS_NON_CORE
    reason: The annotation is compatible with SRC pathway biology but represents a context-specific process,
      location, or phenotype rather than the core kinase function.
- term:
    id: GO:0071375
    label: cellular response to peptide hormone stimulus
  evidence_type: IGI
  original_reference_id: PMID:25017399
  review:
    summary: cellular response to peptide hormone stimulus pathway context
    action: KEEP_AS_NON_CORE
    reason: This term captures a stimulus or pathway context in which SRC can act, not the core SRC molecular
      activity.
- term:
    id: GO:0097110
    label: scaffold protein binding
  evidence_type: IPI
  original_reference_id: PMID:25017399
  review:
    summary: scaffold protein binding supports SRC recruitment or substrate recognition
    action: KEEP_AS_NON_CORE
    reason: This interaction is mechanistically relevant to SRC signaling but is accessory to the core
      tyrosine kinase activity.
- term:
    id: GO:0071560
    label: cellular response to transforming growth factor beta stimulus
  evidence_type: IGI
  original_reference_id: PMID:21829547
  review:
    summary: cellular response to transforming growth factor beta stimulus pathway context
    action: KEEP_AS_NON_CORE
    reason: This term captures a stimulus or pathway context in which SRC can act, not the core SRC molecular
      activity.
- term:
    id: GO:0005884
    label: actin filament
  evidence_type: IDA
  original_reference_id: PMID:17623777
  review:
    summary: actin filament pathway context
    action: KEEP_AS_NON_CORE
    reason: The annotation is compatible with SRC pathway biology but represents a context-specific process,
      location, or phenotype rather than the core kinase function.
- term:
    id: GO:0032587
    label: ruffle membrane
  evidence_type: IDA
  original_reference_id: PMID:17623777
  review:
    summary: ruffle membrane pathway context
    action: KEEP_AS_NON_CORE
    reason: The annotation is compatible with SRC pathway biology but represents a context-specific process,
      location, or phenotype rather than the core kinase function.
- term:
    id: GO:0036120
    label: cellular response to platelet-derived growth factor stimulus
  evidence_type: IDA
  original_reference_id: PMID:17623777
  review:
    summary: cellular response to platelet-derived growth factor stimulus pathway context
    action: KEEP_AS_NON_CORE
    reason: This term captures a stimulus or pathway context in which SRC can act, not the core SRC molecular
      activity.
- term:
    id: GO:0048471
    label: perinuclear region of cytoplasm
  evidence_type: IDA
  original_reference_id: PMID:17623777
  review:
    summary: perinuclear region of cytoplasm pathway context
    action: KEEP_AS_NON_CORE
    reason: The annotation is compatible with SRC pathway biology but represents a context-specific process,
      location, or phenotype rather than the core kinase function.
- term:
    id: GO:0004672
    label: protein kinase activity
  evidence_type: IMP
  original_reference_id: PMID:23100514
  review:
    summary: protein kinase activity is less specific than SRC tyrosine kinase activity
    action: MARK_AS_OVER_ANNOTATED
    reason: The annotation is directionally correct but over-broad because more specific SRC tyrosine
      kinase terms are present.
- term:
    id: GO:0034446
    label: substrate adhesion-dependent cell spreading
  evidence_type: IDA
  original_reference_id: PMID:11864995
  review:
    summary: substrate adhesion-dependent cell spreading pathway context
    action: KEEP_AS_NON_CORE
    reason: The annotation is compatible with SRC pathway biology but represents a context-specific process,
      location, or phenotype rather than the core kinase function.
- term:
    id: GO:0060491
    label: regulation of cell projection assembly
  evidence_type: IGI
  original_reference_id: PMID:11864995
  review:
    summary: regulation of cell projection assembly pathway context
    action: KEEP_AS_NON_CORE
    reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather
      than SRC core molecular activity.
- term:
    id: GO:0035556
    label: intracellular signal transduction
  evidence_type: IGI
  original_reference_id: PMID:23526378
  review:
    summary: SRC intracellular signaling function
    action: KEEP_AS_NON_CORE
    reason: This Lrrk1 osteoclast study supports Src pathway involvement, but intracellular signal
      transduction is broad relative to SRC tyrosine kinase activity.
    supported_by:
    - reference_id: PMID:23526378
      supporting_text: Lrrk1 may function at least in part through modulating the c-Src signaling pathway.
- term:
    id: GO:0036035
    label: osteoclast development
  evidence_type: IGI
  original_reference_id: PMID:23526378
  review:
    summary: osteoclast development pathway context
    action: KEEP_AS_NON_CORE
    reason: The annotation is compatible with SRC pathway biology but represents a context-specific process,
      location, or phenotype rather than the core kinase function.
- term:
    id: GO:0018108
    label: peptidyl-tyrosine phosphorylation
  evidence_type: ISO
  original_reference_id: PMID:22732588
  review:
    summary: PMID:22732588 supports tyrosine phosphorylation of Themis proteins after TCR engagement,
      but the cached text does not directly support Src as the kinase for this annotation.
    action: UNDECIDED
    reason: Full text or a direct Src-family kinase passage would be needed before accepting this ISO
      transfer as Src peptidyl-tyrosine phosphorylation evidence.
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:11826110
  review:
    summary: Generic protein binding
    action: MARK_AS_OVER_ANNOTATED
    reason: Protein binding does not identify a specific SRC substrate-recognition mode or catalytic activity
      and is less informative than SH2/SH3/domain-specific terms.
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:8861907
  review:
    summary: Generic protein binding
    action: MARK_AS_OVER_ANNOTATED
    reason: Protein binding does not identify a specific SRC substrate-recognition mode or catalytic activity
      and is less informative than SH2/SH3/domain-specific terms.
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:21525037
  review:
    summary: Generic protein binding
    action: MARK_AS_OVER_ANNOTATED
    reason: Protein binding does not identify a specific SRC substrate-recognition mode or catalytic activity
      and is less informative than SH2/SH3/domain-specific terms.
- term:
    id: GO:0005886
    label: plasma membrane
  evidence_type: IDA
  original_reference_id: PMID:21525037
  review:
    summary: plasma membrane localization
    action: ACCEPT
    reason: SRC is a soluble non-receptor kinase recruited to cytosolic, membrane, junctional, caveolar,
      and focal-adhesion signaling assemblies.
- term:
    id: GO:0071803
    label: positive regulation of podosome assembly
  evidence_type: IDA
  original_reference_id: PMID:21525037
  review:
    summary: positive regulation of podosome assembly pathway context
    action: KEEP_AS_NON_CORE
    reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather
      than SRC core molecular activity.
- term:
    id: GO:0071803
    label: positive regulation of podosome assembly
  evidence_type: IGI
  original_reference_id: PMID:21525037
  review:
    summary: positive regulation of podosome assembly pathway context
    action: KEEP_AS_NON_CORE
    reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather
      than SRC core molecular activity.
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:14600260
  review:
    summary: Generic protein binding
    action: MARK_AS_OVER_ANNOTATED
    reason: Protein binding does not identify a specific SRC substrate-recognition mode or catalytic activity
      and is less informative than SH2/SH3/domain-specific terms.
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:17000758
  review:
    summary: Generic protein binding
    action: MARK_AS_OVER_ANNOTATED
    reason: Protein binding does not identify a specific SRC substrate-recognition mode or catalytic activity
      and is less informative than SH2/SH3/domain-specific terms.
- term:
    id: GO:0046875
    label: ephrin receptor binding
  evidence_type: IPI
  original_reference_id: PMID:12925710
  review:
    summary: ephrin receptor binding supports SRC recruitment or substrate recognition
    action: KEEP_AS_NON_CORE
    reason: This interaction is mechanistically relevant to SRC signaling but is accessory to the core
      tyrosine kinase activity.
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:14739300
  review:
    summary: Generic protein binding
    action: MARK_AS_OVER_ANNOTATED
    reason: Protein binding does not identify a specific SRC substrate-recognition mode or catalytic activity
      and is less informative than SH2/SH3/domain-specific terms.
- term:
    id: GO:0020037
    label: heme binding
  evidence_type: ISS
  original_reference_id: GO_REF:0000024
  review:
    summary: Generic heme binding annotation
    action: MARK_AS_OVER_ANNOTATED
    reason: The term is too broad or peripheral to capture SRC function when more specific tyrosine kinase
      and signaling annotations are available.
- term:
    id: GO:0045453
    label: bone resorption
  evidence_type: IMP
  original_reference_id: PMID:14739300
  review:
    summary: bone resorption pathway context
    action: KEEP_AS_NON_CORE
    reason: The annotation is compatible with SRC pathway biology but represents a context-specific process,
      location, or phenotype rather than the core kinase function.
- term:
    id: GO:0004672
    label: protein kinase activity
  evidence_type: IDA
  original_reference_id: PMID:19920076
  review:
    summary: protein kinase activity is less specific than SRC tyrosine kinase activity
    action: MARK_AS_OVER_ANNOTATED
    reason: The annotation is directionally correct but over-broad because more specific SRC tyrosine
      kinase terms are present.
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:19920076
  review:
    summary: Generic protein binding
    action: MARK_AS_OVER_ANNOTATED
    reason: Protein binding does not identify a specific SRC substrate-recognition mode or catalytic activity
      and is less informative than SH2/SH3/domain-specific terms.
- term:
    id: GO:0090263
    label: positive regulation of canonical Wnt signaling pathway
  evidence_type: IGI
  original_reference_id: PMID:19920076
  review:
    summary: positive regulation of canonical Wnt signaling pathway pathway context
    action: KEEP_AS_NON_CORE
    reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather
      than SRC core molecular activity.
- term:
    id: GO:0005829
    label: cytosol
  evidence_type: TAS
  original_reference_id: Reactome:R-MMU-1433273
  review:
    summary: cytosol localization
    action: ACCEPT
    reason: SRC is a soluble non-receptor kinase recruited to cytosolic, membrane, junctional, caveolar,
      and focal-adhesion signaling assemblies.
- term:
    id: GO:0005829
    label: cytosol
  evidence_type: TAS
  original_reference_id: Reactome:R-MMU-3928622
  review:
    summary: cytosol localization
    action: ACCEPT
    reason: SRC is a soluble non-receptor kinase recruited to cytosolic, membrane, junctional, caveolar,
      and focal-adhesion signaling assemblies.
- term:
    id: GO:0005829
    label: cytosol
  evidence_type: TAS
  original_reference_id: Reactome:R-MMU-418870
  review:
    summary: cytosol localization
    action: ACCEPT
    reason: SRC is a soluble non-receptor kinase recruited to cytosolic, membrane, junctional, caveolar,
      and focal-adhesion signaling assemblies.
- term:
    id: GO:0005829
    label: cytosol
  evidence_type: TAS
  original_reference_id: Reactome:R-MMU-420382
  review:
    summary: cytosol localization
    action: ACCEPT
    reason: SRC is a soluble non-receptor kinase recruited to cytosolic, membrane, junctional, caveolar,
      and focal-adhesion signaling assemblies.
- term:
    id: GO:0005829
    label: cytosol
  evidence_type: TAS
  original_reference_id: Reactome:R-MMU-5218834
  review:
    summary: cytosol localization
    action: ACCEPT
    reason: SRC is a soluble non-receptor kinase recruited to cytosolic, membrane, junctional, caveolar,
      and focal-adhesion signaling assemblies.
- term:
    id: GO:0005829
    label: cytosol
  evidence_type: TAS
  original_reference_id: Reactome:R-MMU-8964229
  review:
    summary: cytosol localization
    action: ACCEPT
    reason: SRC is a soluble non-receptor kinase recruited to cytosolic, membrane, junctional, caveolar,
      and focal-adhesion signaling assemblies.
- term:
    id: GO:0005829
    label: cytosol
  evidence_type: TAS
  original_reference_id: Reactome:R-MMU-9680646
  review:
    summary: cytosol localization
    action: ACCEPT
    reason: SRC is a soluble non-receptor kinase recruited to cytosolic, membrane, junctional, caveolar,
      and focal-adhesion signaling assemblies.
- term:
    id: GO:0005829
    label: cytosol
  evidence_type: TAS
  original_reference_id: Reactome:R-MMU-9680706
  review:
    summary: cytosol localization
    action: ACCEPT
    reason: SRC is a soluble non-receptor kinase recruited to cytosolic, membrane, junctional, caveolar,
      and focal-adhesion signaling assemblies.
- term:
    id: GO:0005829
    label: cytosol
  evidence_type: TAS
  original_reference_id: Reactome:R-MMU-9682158
  review:
    summary: cytosol localization
    action: ACCEPT
    reason: SRC is a soluble non-receptor kinase recruited to cytosolic, membrane, junctional, caveolar,
      and focal-adhesion signaling assemblies.
- term:
    id: GO:0005829
    label: cytosol
  evidence_type: TAS
  original_reference_id: Reactome:R-MMU-9682182
  review:
    summary: cytosol localization
    action: ACCEPT
    reason: SRC is a soluble non-receptor kinase recruited to cytosolic, membrane, junctional, caveolar,
      and focal-adhesion signaling assemblies.
- term:
    id: GO:0005829
    label: cytosol
  evidence_type: TAS
  original_reference_id: Reactome:R-MMU-9682572
  review:
    summary: cytosol localization
    action: ACCEPT
    reason: SRC is a soluble non-receptor kinase recruited to cytosolic, membrane, junctional, caveolar,
      and focal-adhesion signaling assemblies.
- term:
    id: GO:0005829
    label: cytosol
  evidence_type: TAS
  original_reference_id: Reactome:R-MMU-9763891
  review:
    summary: cytosol localization
    action: ACCEPT
    reason: SRC is a soluble non-receptor kinase recruited to cytosolic, membrane, junctional, caveolar,
      and focal-adhesion signaling assemblies.
- term:
    id: GO:0005829
    label: cytosol
  evidence_type: TAS
  original_reference_id: Reactome:R-MMU-9763892
  review:
    summary: cytosol localization
    action: ACCEPT
    reason: SRC is a soluble non-receptor kinase recruited to cytosolic, membrane, junctional, caveolar,
      and focal-adhesion signaling assemblies.
- term:
    id: GO:0005829
    label: cytosol
  evidence_type: TAS
  original_reference_id: Reactome:R-MMU-9763903
  review:
    summary: cytosol localization
    action: ACCEPT
    reason: SRC is a soluble non-receptor kinase recruited to cytosolic, membrane, junctional, caveolar,
      and focal-adhesion signaling assemblies.
- term:
    id: GO:0005829
    label: cytosol
  evidence_type: TAS
  original_reference_id: Reactome:R-MMU-9764150
  review:
    summary: cytosol localization
    action: ACCEPT
    reason: SRC is a soluble non-receptor kinase recruited to cytosolic, membrane, junctional, caveolar,
      and focal-adhesion signaling assemblies.
- term:
    id: GO:0005829
    label: cytosol
  evidence_type: TAS
  original_reference_id: Reactome:R-MMU-9817994
  review:
    summary: cytosol localization
    action: ACCEPT
    reason: SRC is a soluble non-receptor kinase recruited to cytosolic, membrane, junctional, caveolar,
      and focal-adhesion signaling assemblies.
- term:
    id: GO:0005829
    label: cytosol
  evidence_type: TAS
  original_reference_id: Reactome:R-MMU-9818009
  review:
    summary: cytosol localization
    action: ACCEPT
    reason: SRC is a soluble non-receptor kinase recruited to cytosolic, membrane, junctional, caveolar,
      and focal-adhesion signaling assemblies.
- term:
    id: GO:0005829
    label: cytosol
  evidence_type: TAS
  original_reference_id: Reactome:R-NUL-9700661
  review:
    summary: cytosol localization
    action: ACCEPT
    reason: SRC is a soluble non-receptor kinase recruited to cytosolic, membrane, junctional, caveolar,
      and focal-adhesion signaling assemblies.
- term:
    id: GO:0070374
    label: positive regulation of ERK1 and ERK2 cascade
  evidence_type: IMP
  original_reference_id: PMID:17045821
  review:
    summary: positive regulation of ERK1 and ERK2 cascade pathway context
    action: KEEP_AS_NON_CORE
    reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather
      than SRC core molecular activity.
- term:
    id: GO:0033146
    label: regulation of intracellular estrogen receptor signaling pathway
  evidence_type: IMP
  original_reference_id: PMID:16007215
  review:
    summary: regulation of intracellular estrogen receptor signaling pathway pathway context
    action: KEEP_AS_NON_CORE
    reason: This regulatory term is a downstream or pathway-specific consequence of SRC signaling rather
      than SRC core molecular activity.
- term:
    id: GO:0048477
    label: oogenesis
  evidence_type: IMP
  original_reference_id: PMID:16007215
  review:
    summary: oogenesis pathway context
    action: KEEP_AS_NON_CORE
    reason: The annotation is compatible with SRC pathway biology but represents a context-specific process,
      location, or phenotype rather than the core kinase function.
- term:
    id: GO:0060065
    label: uterus development
  evidence_type: IMP
  original_reference_id: PMID:16007215
  review:
    summary: uterus development pathway context
    action: KEEP_AS_NON_CORE
    reason: The annotation is compatible with SRC pathway biology but represents a context-specific process,
      location, or phenotype rather than the core kinase function.
- term:
    id: GO:0060444
    label: branching involved in mammary gland duct morphogenesis
  evidence_type: IMP
  original_reference_id: PMID:16007215
  review:
    summary: branching involved in mammary gland duct morphogenesis pathway context
    action: KEEP_AS_NON_CORE
    reason: The annotation is compatible with SRC pathway biology but represents a context-specific process,
      location, or phenotype rather than the core kinase function.
- term:
    id: GO:0019904
    label: protein domain specific binding
  evidence_type: IPI
  original_reference_id: PMID:8438166
  review:
    summary: protein domain specific binding supports SRC recruitment or substrate recognition
    action: KEEP_AS_NON_CORE
    reason: This interaction is mechanistically relevant to SRC signaling but is accessory to the core
      tyrosine kinase activity.
- term:
    id: GO:0006468
    label: protein phosphorylation
  evidence_type: IDA
  original_reference_id: PMID:15937334
  review:
    summary: PMID:15937334 supports chronological activation of Src-family tyrosine kinases in P2X7R-mediated
      thymocyte death, but does not identify Src specifically in the cached abstract.
    action: UNDECIDED
    reason: Full text would be needed to confirm this as a direct Src protein-phosphorylation annotation.
- term:
    id: GO:0016477
    label: cell migration
  evidence_type: IMP
  original_reference_id: PMID:15292044
  review:
    summary: cell migration pathway context
    action: KEEP_AS_NON_CORE
    reason: The annotation is compatible with SRC pathway biology but represents a context-specific process,
      location, or phenotype rather than the core kinase function.
- term:
    id: GO:0018108
    label: peptidyl-tyrosine phosphorylation
  evidence_type: IDA
  original_reference_id: PMID:12526740
  review:
    summary: PMID:12526740 shows Reelin-dependent activation of Src-family non-receptor tyrosine
      kinases in neurons, with Dab1 tyrosine phosphorylation by SFKs.
    action: ACCEPT
    reason: The current term is already the tyrosine-specific phosphorylation process supported
      by the Reelin/Src-family kinase study, so no replacement is needed.
    supported_by:
    - reference_id: PMID:12526740
      supporting_text: "Dab1 is tyrosine phosphorylated by SFKs"
- term:
    id: GO:0030900
    label: forebrain development
  evidence_type: IGI
  original_reference_id: PMID:16162939
  review:
    summary: forebrain development pathway context
    action: KEEP_AS_NON_CORE
    reason: The annotation is compatible with SRC pathway biology but represents a context-specific process,
      location, or phenotype rather than the core kinase function.
- term:
    id: GO:0016301
    label: kinase activity
  evidence_type: IMP
  original_reference_id: PMID:12808090
  review:
    summary: kinase activity is less specific than SRC tyrosine kinase activity
    action: MARK_AS_OVER_ANNOTATED
    reason: The annotation is directionally correct but over-broad because more specific SRC tyrosine
      kinase terms are present.
- term:
    id: GO:0004713
    label: protein tyrosine kinase activity
  evidence_type: IMP
  original_reference_id: PMID:14636584
  review:
    summary: Core SRC non-receptor tyrosine kinase activity
    action: ACCEPT
    reason: SRC is a non-receptor tyrosine kinase; tyrosine phosphorylation of protein substrates is its
      primary molecular activity.
- term:
    id: GO:0004672
    label: protein kinase activity
  evidence_type: IDA
  original_reference_id: PMID:11274221
  review:
    summary: protein kinase activity is less specific than SRC tyrosine kinase activity
    action: MARK_AS_OVER_ANNOTATED
    reason: The annotation is directionally correct but over-broad because more specific SRC tyrosine
      kinase terms are present.
- term:
    id: GO:0006468
    label: protein phosphorylation
  evidence_type: IDA
  original_reference_id: PMID:11274221
  review:
    summary: Direct SRC tyrosine phosphorylation activity
    action: ACCEPT
    reason: This term directly describes the catalytic output of SRC kinase activity on protein tyrosine
      residues.
references:
- id: GO_REF:0000002
  title: Gene Ontology annotation through association of InterPro records with GO terms
  findings: []
- id: GO_REF:0000024
  title: Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator
    judgment of sequence similarity
  findings: []
- id: GO_REF:0000033
  title: Annotation inferences using phylogenetic trees
  findings: []
- id: GO_REF:0000044
  title: Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping,
    accompanied by conservative changes to GO terms applied by UniProt
  findings: []
- id: GO_REF:0000096
  title: Automated transfer of experimentally-verified manual GO annotation data to mouse-rat orthologs
  findings: []
- id: GO_REF:0000107
  title: Automatic transfer of experimentally verified manual GO annotation data to orthologs using Ensembl
    Compara
  findings: []
- id: GO_REF:0000117
  title: Electronic Gene Ontology annotations created by ARBA machine learning models
  findings: []
- id: GO_REF:0000119
  title: Automated transfer of experimentally-verified manual GO annotation data to mouse-human orthologs
  findings: []
- id: GO_REF:0000120
  title: Combined Automated Annotation using Multiple IEA Methods
  findings: []
- id: PMID:10220404
  title: Identification of profilin and src homology 3 domains as binding partners for Drosophila enabled.
  findings: []
- id: PMID:11274221
  title: Src and Pyk2 mediate G-protein-coupled receptor activation of epidermal growth factor receptor
    (EGFR) but are not required for coupling to the mitogen-activated protein (MAP) kinase signaling cascade.
  findings: []
- id: PMID:11826110
  title: 'Delphilin: a novel PDZ and formin homology domain-containing protein that synaptically colocalizes
    and interacts with glutamate receptor delta 2 subunit.'
  findings: []
- id: PMID:11864995
  title: Modulation of the F-actin cytoskeleton by c-Abl tyrosine kinase in cell spreading and neurite
    extension.
  findings: []
- id: PMID:12091389
  title: Src-induced phosphorylation of caveolin-2 on tyrosine 19. Phospho-caveolin-2 (Tyr(P)19) is localized
    near focal adhesions, remains associated with lipid rafts/caveolae, but no longer forms a high molecular
    mass hetero-oligomer with caveolin-1.
  findings: []
- id: PMID:12526740
  title: Reelin activates SRC family tyrosine kinases in neurons.
  findings:
  - statement: Reelin activates Src-family non-receptor tyrosine kinases in neurons and supports Dab1 tyrosine phosphorylation as Src-family kinase output
    supporting_text: "We show that Reelin activates members of the Src family of non-receptor tyrosine kinases (SFKs)"
- id: PMID:12615910
  title: Regulation of cytochrome c oxidase activity by c-Src in osteoclasts.
  findings: []
- id: PMID:12808090
  title: Gab1 is an integrator of cell death versus cell survival signals in oxidative stress.
  findings: []
- id: PMID:12925710
  title: EphB1 recruits c-Src and p52Shc to activate MAPK/ERK and promote chemotaxis.
  findings: []
- id: PMID:14600260
  title: A caveolin-3 mutant that causes limb girdle muscular dystrophy type 1C disrupts Src localization
    and activity and induces apoptosis in skeletal myotubes.
  findings: []
- id: PMID:14636584
  title: Phosphorylation-dependent paxillin-ERK association mediates hepatocyte growth factor-stimulated
    epithelial morphogenesis.
  findings: []
- id: PMID:14699011
  title: Regulation of connexin43 protein complexes by intracellular acidification.
  findings: []
- id: PMID:14739300
  title: Src kinase activity is essential for osteoclast function.
  findings: []
- id: PMID:15292044
  title: Alterations in cell-adhesive and migratory properties of proximal tubule and collecting duct
    cells from bcl-2 -/- mice.
  findings: []
- id: PMID:15937334
  title: A role for mitogen-activated protein kinase(Erk1/2) activation and non-selective pore formation
    in P2X7 receptor-mediated thymocyte death.
  findings: []
- id: PMID:16007215
  title: c-Src-null mice exhibit defects in normal mammary gland development and ERalpha signaling.
  findings: []
- id: PMID:16162939
  title: Absence of Fyn and Src causes a reeler-like phenotype.
  findings: []
- id: PMID:16267818
  title: A proteomics strategy for the enrichment of receptor-associated complexes.
  findings: []
- id: PMID:16298995
  title: EphB receptors regulate dendritic spine morphogenesis through the recruitment/phosphorylation
    of focal adhesion kinase and RhoA activation.
  findings: []
- id: PMID:16407161
  title: Role of Src in C3 transient receptor potential channel function and evidence for a heterogeneous
    makeup of receptor- and store-operated Ca2+ entry channels.
  findings: []
- id: PMID:16990796
  title: PSD-95 is a negative regulator of the tyrosine kinase Src in the NMDA receptor complex.
  findings: []
- id: PMID:17000758
  title: Ccpg1, a novel scaffold protein that regulates the activity of the Rho guanine nucleotide exchange
    factor Dbs.
  findings: []
- id: PMID:17045821
  title: CD44 is the signaling component of the macrophage migration inhibitory factor-CD74 receptor complex.
  findings: []
- id: PMID:17344476
  title: The transcriptional repressor Glis2 is a novel binding partner for p120 catenin.
  findings: []
- id: PMID:17623777
  title: The membrane targeting and spatial activation of Src, Yes and Fyn is influenced by palmitoylation
    and distinct RhoB/RhoD endosome requirements.
  findings: []
- id: PMID:17893324
  title: Src-dependent phosphorylation of ASAP1 regulates podosomes.
  findings: []
- id: PMID:18044967
  title: Laminin-induced activation of Rac1 and JNKp46 is initiated by Src family kinases and mimics the
    effects of skeletal muscle contraction.
  findings: []
- id: PMID:18289379
  title: Dramatic inhibition of osteoclast sealing ring formation and bone resorption in vitro by a WASP-peptide
    containing pTyr294 amino acid.
  findings: []
- id: PMID:18560762
  title: Mobility of TOAC spin-labelled peptides binding to the Src SH3 domain studied by paramagnetic
    NMR.
  findings: []
- id: PMID:18691334
  title: Palmitoylation-dependent endosomal localization of AATYK1A and its interaction with Src.
  findings: []
- id: PMID:19122674
  title: Deficiency of a beta-arrestin-2 signal complex contributes to insulin resistance.
  findings: []
- id: PMID:19448635
  title: Impaired gastric acidification negatively affects calcium homeostasis and bone mass.
  findings: []
- id: PMID:19920076
  title: Dishevelled-2 docks and activates Src in a Wnt-dependent manner.
  findings: []
- id: PMID:20847314
  title: CXCR4-mediated bone marrow progenitor cell maintenance and mobilization are modulated by c-kit
    activity.
  findings: []
- id: PMID:21199370
  title: Roles of the SH2 and SH3 domains in the regulation of neuronal Src kinase functions.
  findings: []
- id: PMID:21266176
  title: Linker length dependent binding of a focal adhesion kinase derived peptide to the Src SH3-SH2
    domains.
  findings: []
- id: PMID:21282606
  title: Nε-lysine acetylation determines dissociation from GAP junctions and lateralization of connexin
    43 in normal and dystrophic heart.
  findings: []
- id: PMID:21525037
  title: The guanine nucleotide exchange factor Arhgef5 plays crucial roles in Src-induced podosome formation.
  findings: []
- id: PMID:21829547
  title: Redox-induced Src kinase and caveolin-1 signaling in TGF-β1-initiated SMAD2/3 activation and
    PAI-1 expression.
  findings: []
- id: PMID:22439932
  title: NKX2-1/TITF1/TTF-1-Induced ROR1 is required to sustain EGFR survival signaling in lung adenocarcinoma.
  findings: []
- id: PMID:22732588
  title: Interchangeability of Themis1 and Themis2 in thymocyte development reveals two related proteins
    with conserved molecular function.
  findings: []
- id: PMID:22759635
  title: A Src family kinase-Shp2 axis controls RUNX1 activity in megakaryocyte and T-lymphocyte differentiation.
  findings: []
- id: PMID:22801373
  title: Protein tyrosine phosphatase α phosphotyrosyl-789 binds BCAR3 to position Cas for activation
    at integrin-mediated focal adhesions.
  findings: []
- id: PMID:23100514
  title: Abl family kinases regulate FcγR-mediated phagocytosis in murine macrophages.
  findings: []
- id: PMID:23174213
  title: Osteopetrosis, osteopetrorickets and hypophosphatemic rickets differentially affect dentin and
    enamel mineralization.
  findings: []
- id: PMID:23526378
  title: Targeted disruption of leucine-rich repeat kinase 1 but not leucine-rich repeat kinase 2 in mice
    causes severe osteopetrosis.
  findings: []
- id: PMID:23651497
  title: A signaling cascade mediated by ceramide, src and PDGFRβ coordinates the activation of the redox-sensitive
    neutral sphingomyelinase-2 and sphingosine kinase-1.
  findings: []
- id: PMID:24127566
  title: Phosphorylation of moesin by Jun N-terminal kinase is important for podosome rosette formation
    in Src-transformed fibroblasts.
  findings: []
- id: PMID:24190966
  title: A reactive oxygen species-mediated, self-perpetuating loop persistently activates platelet-derived
    growth factor receptor α.
  findings: []
- id: PMID:24841674
  title: Mechanical loading in osteocytes induces formation of a Src/Pyk2/MBD2 complex that suppresses
    anabolic gene expression.
  findings: []
- id: PMID:25017399
  title: Caveolin-1 modulates cardiac gap junction homeostasis and arrhythmogenecity by regulating cSrc
    tyrosine kinase.
  findings: []
- id: PMID:25043309
  title: Actin filament-associated protein 1 is required for cSrc activity and secretory activation in
    the lactating mammary gland.
  findings: []
- id: PMID:25259869
  title: Genetic disruption of the sh3pxd2a gene reveals an essential role in mouse development and the
    existence of a novel isoform of tks5.
  findings: []
- id: PMID:25731159
  title: A gp130-Src-YAP module links inflammation to epithelial regeneration.
  findings: []
- id: PMID:27505886
  title: Tensin 3 is a new partner of Dock5 that controls osteoclast podosome organization and activity.
  findings: []
- id: PMID:27994061
  title: Focal Adhesion Kinase-mediated Phosphorylation of Beclin1 Protein Suppresses Cardiomyocyte Autophagy
    and Initiates Hypertrophic Growth.
  findings: []
- id: PMID:28054552
  title: c-Src phosphorylation and activation of hexokinase promotes tumorigenesis and metastasis.
  findings: []
- id: PMID:35263589
  title: Nociceptor-derived Reg3γ prevents endotoxic death by targeting kynurenine pathway in microglia.
  findings: []
- id: PMID:7518772
  title: Phosphorylation of receptor protein-tyrosine phosphatase alpha on Tyr789, a binding site for
    the SH3-SH2-SH3 adaptor protein GRB-2 in vivo.
  findings: []
- id: PMID:8438166
  title: Identification of a ten-amino acid proline-rich SH3 binding site.
  findings: []
- id: PMID:8861907
  title: Mena, a relative of VASP and Drosophila Enabled, is implicated in the control of microfilament
    dynamics.
  findings: []
- id: PMID:8910389
  title: Bombesin, bradykinin, vasopressin, and phorbol esters rapidly and transiently activate Src family
    tyrosine kinases in Swiss 3T3 cells. Dissociation from tyrosine phosphorylation of p125 focal adhesion
    kinase.
  findings: []
- id: Reactome:R-MMU-1433273
  title: Interaction of Src kinases with c-Kit
  findings: []
- id: Reactome:R-MMU-3928622
  title: SFKs phosphorylate Git1
  findings: []
- id: Reactome:R-MMU-418870
  title: Phosphorylation of Unc5C
  findings: []
- id: Reactome:R-MMU-420382
  title: Phosphorylation of FAK by Src kinases
  findings: []
- id: Reactome:R-MMU-5218834
  title: Fak/Src phosphorylate p130Cas
  findings: []
- id: Reactome:R-MMU-8937703
  title: Runx1 binds Src
  findings: []
- id: Reactome:R-MMU-8937738
  title: Src phosphorylates Runx1
  findings: []
- id: Reactome:R-MMU-8964229
  title: G alpha (i):GTP binds Src
  findings: []
- id: Reactome:R-MMU-9011252
  title: Src phosphorylates Robo3.1 in response to Ntn1
  findings: []
- id: Reactome:R-MMU-9680646
  title: Pik3r11:Pik3ca,b,d (Pi3k), Plcg2 (PLCgamma2), Grb2:Sos1, Shc1 (Shc), Ptpn11 (Shp2), Grb2:Gab2,
    Grb2:Gab3, Grap2 (MONA), Cbl:Grb2, Inpp5d (SHIP1), Inppl1 (SHIP2)  bind p-8Y-Csf1r and are activated
  findings: []
- id: Reactome:R-MMU-9680706
  title: Csf1r trans-autophosphorylates on multiple tyrosine residues
  findings: []
- id: Reactome:R-MMU-9682158
  title: Csf1r-associated Plcg2 hydrolyzes phosphatidylcholine yielding choline phosphate and 1,2-diacylglycerol
  findings: []
- id: Reactome:R-MMU-9682182
  title: Csf1r-associated PI3K phosphorylates phosphatidylinositol 4,5-bisphosphate
  findings: []
- id: Reactome:R-MMU-9682572
  title: Csf1r-associated Sos1 mediates exchange of GTP for GDP bound to Ras
  findings: []
- id: Reactome:R-MMU-9763891
  title: Src family kinases phosphorylate Cbl in Csf1 dimer:p-Y559-Csf1r dimer:Cbl
  findings: []
- id: Reactome:R-MMU-9763892
  title: p-Y-Cbl autoubiquitinates and multiubiquitinates p-Y-559-Csf12
  findings: []
- id: Reactome:R-MMU-9763903
  title: Csf1 dimer:p-Y559-Csf1r dimer binds Grb2:Sos1
  findings: []
- id: Reactome:R-MMU-9764150
  title: Csf1 dimer:p-Y-559-Csf1r dimer:SFK:Grb2:Sos1 dissociates yielding Csf1 dimer:p-Y-559-Csf1r dimer:SFK
    and Grb2:Sos1
  findings: []
- id: Reactome:R-MMU-9817994
  title: Csf1 dimer:p-Y559-Csf1r dimer:Fyn,Hck,Src,Yes1 binds Cbl
  findings: []
- id: Reactome:R-MMU-9818009
  title: Csf1 dimer:p-Y559-Csf1r dimer binds Src family kinases Fyn,Hck,Src,Yes1
  findings: []
- id: Reactome:R-NUL-9700655
  title: Active ALK phosphorylates Src
  findings: []
- id: Reactome:R-NUL-9700661
  title: Active ALK binds Src
  findings: []
- id: Reactome:R-NUL-9726861
  title: Src phosphorylates RHOU
  findings: []
- id: file:mouse/Src/Src-deep-research-bioreason.md
  title: BioReason deep research report on Src
  findings: []
- id: file:mouse/Src/Src-deep-research-falcon.md
  title: Falcon deep research summary for mouse Src
  findings:
  - statement: Src is a membrane-associated non-receptor tyrosine kinase with SH3,
      SH2, and catalytic kinase domains.
  - statement: Falcon synthesis supports Src-mediated tyrosine phosphorylation in receptor,
      adhesion, and cytoskeletal signaling complexes.
- id: file:mouse/Src/Src-notes.md
  title: AIGR curator notes for Src
  findings: []
core_functions:
- molecular_function:
    id: GO:0004715
    label: non-membrane spanning protein tyrosine kinase activity
  description: SRC phosphorylates protein tyrosine residues as a regulated non-receptor kinase assembled
    with receptor, adaptor, and cytoskeletal signaling complexes through SH2 and SH3 domain interactions.
  locations:
  - id: GO:0005829
    label: cytosol
  - id: GO:0005886
    label: plasma membrane
  - id: GO:0005925
    label: focal adhesion
  directly_involved_in:
  - id: GO:0018108
    label: peptidyl-tyrosine phosphorylation
  - id: GO:0035556
    label: intracellular signal transduction
  supported_by:
  - reference_id: file:mouse/Src/Src-notes.md
    supporting_text: SRC is a non-receptor protein tyrosine kinase with SH3-SH2-kinase architecture.
  - reference_id: file:mouse/Src/Src-deep-research-falcon.md
    supporting_text: Falcon synthesis supports Src as a regulated non-receptor tyrosine kinase that
      phosphorylates protein substrates in receptor, adhesion, and cytoskeletal signaling complexes.

Src

Gene Description

Existing Annotations Review

Core Functions

SRC phosphorylates protein tyrosine residues as a regulated non-receptor kinase assembled with receptor, adaptor, and cytoskeletal signaling complexes through SH2 and SH3 domain interactions.

References

📚 Additional Documentation

Deep Research Bioreason

BioReason Chat Export

Thinking Trace

Functional Summary

UniProt Summary

InterPro Domains

GO Terms

Deep Research Bioreason Rl

BioReason Chat Export

Thinking Trace

Functional Summary

UniProt Summary

InterPro Domains

GO Terms

Deep Research Falcon

Question

Gene Research for Functional Annotation

⚠️ CRITICAL: Gene/Protein Identification Context

Target Gene/Protein Identity (from UniProt):

MANDATORY VERIFICATION STEPS:

If Gene Symbol is Ambiguous or You Cannot Find Relevant Literature:

Research Target:

Output

Gene Research for Functional Annotation

⚠️ CRITICAL: Gene/Protein Identification Context

Target Gene/Protein Identity (from UniProt):

MANDATORY VERIFICATION STEPS:

If Gene Symbol is Ambiguous or You Cannot Find Relevant Literature:

Research Target:

Research report: Mouse Src (UniProt P05480) functional annotation

0) Mandatory verification: correct gene/protein

1) Key concepts and definitions (current understanding)

1.1 What Src is

1.2 Enzymatic function (reaction and substrates)

1.3 Regulation: the “Src switch” (closed/inactive vs open/active)

1.4 Substrate specificity (what Src phosphorylates and why)

2) Subcellular localization (where Src acts)

3) Biological processes and pathways (functional annotation)

3.1 Adhesion and cytoskeletal signaling (FAK/paxillin/p130Cas axis)

3.2 Wnt signaling crosstalk

3.3 Oncogenic signal transduction programs

4) Genetics and in vivo function in mouse: src−/− phenotypes (real-world functional evidence)

5) Recent developments and latest research (prioritizing 2023–2024)

5.1 2023: New allosteric model of Src autophosphorylation and C-terminal regulation

5.2 2024: c-SRC rewires lipid metabolism in glioblastoma via ACSS2/ACLY phosphorylation

5.3 2024: Chemoresistant TNBC exhibits SFK upregulation and actionable inhibitor sensitivity

5.4 2024: Saracatinib (AZD0530) as a Src-directed tool compound and repurposing candidate

5.5 2024: Stromal targeting via Src inhibition in gastric cancer–activated CAFs

6) Current applications and real-world implementations

6.1 Mouse genetics as functional validation tools

6.2 Disease models and translational frameworks

6.3 Pharmacologic inhibition in practice

7) Expert synthesis and analysis (authoritative perspectives)

7.1 Mechanistic consensus: modular “Src module” principles

7.2 Emerging consensus: regulation extends beyond the classic two-tyrosine switch

7.3 Translational note: SFK signaling as a recurring resistance node

8) Evidence map (summary table)

9) Key statistics and data (recent and classic; selected)

References (URLs and publication dates)

Citations

Notes

Src review notes

Bioreason Rl Review

BioReason-Pro RL Review: Src (mouse)

Functional Summary Review

Notes on thinking trace

📄 View Raw YAML