Mouse Trp53 encodes tumor suppressor p53, a stress-activated, sequence-specific DNA-binding transcription factor. p53 binds response elements as a tetramer and regulates gene programs controlling cell-cycle arrest, apoptosis, senescence, DNA repair, metabolism, and other stress responses. Its core role is nuclear transcriptional control, with additional context-dependent mitochondrial, cytoplasmic, and tissue-specific outputs.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0000981 DNA-binding transcription factor activity, RNA polymerase II-specific | IBA GO_REF:0000033 | ACCEPT | Summary: DNA-binding transcription factor activity, RNA polymerase II-specific is consistent with the curated mouse p53 biology as a stress-activated DNA-binding transcription factor, transcriptional regulator, or well-supported subcellular context. Reason: The term fits the direct p53 activity or a well-established cellular context for that activity, supported by UniProt and the Falcon literature synthesis. |
| GO:0042771 intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator | IBA GO_REF:0000033 | ACCEPT | Summary: intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator is consistent with the curated mouse p53 biology as a stress-activated DNA-binding transcription factor, transcriptional regulator, or well-supported subcellular context. Reason: The term fits the direct p53 activity or a well-established cellular context for that activity, supported by UniProt and the Falcon literature synthesis. |
| GO:0042981 regulation of apoptotic process | IBA GO_REF:0000033 | KEEP AS NON CORE | Summary: regulation of apoptotic process is a broad downstream p53 cell-fate annotation rather than the core Trp53 molecular function. Reason: p53 can induce apoptotic programs after stress, but broad apoptosis terms should be retained as non-core because the core Trp53 function is sequence-specific DNA-binding transcriptional regulation. Supporting Evidence: file:mouse/Trp53/Trp53-deep-research-falcon.md Canonical apoptotic targets include Bbc3/Puma, Bax, Pmaip1/Noxa, and Apaf1. |
| GO:1990841 promoter-specific chromatin binding | IBA GO_REF:0000033 | ACCEPT | Summary: promoter-specific chromatin binding is consistent with the curated mouse p53 biology as a stress-activated DNA-binding transcription factor, transcriptional regulator, or well-supported subcellular context. Reason: The term fits the direct p53 activity or a well-established cellular context for that activity, supported by UniProt and the Falcon literature synthesis. |
| GO:0005634 nucleus | IBA GO_REF:0000033 | ACCEPT | Summary: nucleus is consistent with the curated mouse p53 biology as a stress-activated DNA-binding transcription factor, transcriptional regulator, or well-supported subcellular context. Reason: The term fits the direct p53 activity or a well-established cellular context for that activity, supported by UniProt and the Falcon literature synthesis. |
| GO:0045944 positive regulation of transcription by RNA polymerase II | IBA GO_REF:0000033 | ACCEPT | Summary: positive regulation of transcription by RNA polymerase II is consistent with the curated mouse p53 biology as a stress-activated DNA-binding transcription factor, transcriptional regulator, or well-supported subcellular context. Reason: The term fits the direct p53 activity or a well-established cellular context for that activity, supported by UniProt and the Falcon literature synthesis. |
| GO:0000785 chromatin | IBA GO_REF:0000033 | ACCEPT | Summary: chromatin is consistent with the curated mouse p53 biology as a stress-activated DNA-binding transcription factor, transcriptional regulator, or well-supported subcellular context. Reason: The term fits the direct p53 activity or a well-established cellular context for that activity, supported by UniProt and the Falcon literature synthesis. |
| GO:0000978 RNA polymerase II cis-regulatory region sequence-specific DNA binding | IBA GO_REF:0000033 | ACCEPT | Summary: RNA polymerase II cis-regulatory region sequence-specific DNA binding is consistent with the curated mouse p53 biology as a stress-activated DNA-binding transcription factor, transcriptional regulator, or well-supported subcellular context. Reason: The term fits the direct p53 activity or a well-established cellular context for that activity, supported by UniProt and the Falcon literature synthesis. |
| GO:0000976 transcription cis-regulatory region binding | IEA GO_REF:0000120 | ACCEPT | Summary: transcription cis-regulatory region binding is consistent with the curated mouse p53 biology as a stress-activated DNA-binding transcription factor, transcriptional regulator, or well-supported subcellular context. Reason: The term fits the direct p53 activity or a well-established cellular context for that activity, supported by UniProt and the Falcon literature synthesis. |
| GO:0003677 DNA binding | IEA GO_REF:0000120 | ACCEPT | Summary: DNA binding is consistent with the curated mouse p53 biology as a stress-activated DNA-binding transcription factor, transcriptional regulator, or well-supported subcellular context. Reason: The term fits the direct p53 activity or a well-established cellular context for that activity, supported by UniProt and the Falcon literature synthesis. |
| GO:0003700 DNA-binding transcription factor activity | IEA GO_REF:0000120 | ACCEPT | Summary: DNA-binding transcription factor activity is consistent with the curated mouse p53 biology as a stress-activated DNA-binding transcription factor, transcriptional regulator, or well-supported subcellular context. Reason: The term fits the direct p53 activity or a well-established cellular context for that activity, supported by UniProt and the Falcon literature synthesis. |
| GO:0005634 nucleus | IEA GO_REF:0000120 | ACCEPT | Summary: nucleus is consistent with the curated mouse p53 biology as a stress-activated DNA-binding transcription factor, transcriptional regulator, or well-supported subcellular context. Reason: The term fits the direct p53 activity or a well-established cellular context for that activity, supported by UniProt and the Falcon literature synthesis. |
| GO:0005737 cytoplasm | IEA GO_REF:0000120 | KEEP AS NON CORE | Summary: cytoplasm is a supported p53-associated context, interaction, localization, or downstream output, but it is not the core Trp53 molecular function. Reason: Retain as non-core because Trp53/p53 is primarily a sequence-specific DNA-binding transcription factor; this term reflects regulatory context, stress response, localization, or a tissue-specific downstream consequence. Supporting Evidence: file:mouse/Trp53/Trp53-deep-research-falcon.md The p53 transcriptional program is tissue-, stress-, and context-dependent; many annotated responses are downstream or specialized outputs rather than the core molecular activity. |
| GO:0005759 mitochondrial matrix | IEA GO_REF:0000044 | KEEP AS NON CORE | Summary: mitochondrial matrix is a supported p53-associated context, interaction, localization, or downstream output, but it is not the core Trp53 molecular function. Reason: Retain as non-core because Trp53/p53 is primarily a sequence-specific DNA-binding transcription factor; this term reflects regulatory context, stress response, localization, or a tissue-specific downstream consequence. Supporting Evidence: file:mouse/Trp53/Trp53-deep-research-falcon.md The p53 transcriptional program is tissue-, stress-, and context-dependent; many annotated responses are downstream or specialized outputs rather than the core molecular activity. |
| GO:0005783 endoplasmic reticulum | IEA GO_REF:0000044 | KEEP AS NON CORE | Summary: endoplasmic reticulum is a supported p53-associated context, interaction, localization, or downstream output, but it is not the core Trp53 molecular function. Reason: Retain as non-core because Trp53/p53 is primarily a sequence-specific DNA-binding transcription factor; this term reflects regulatory context, stress response, localization, or a tissue-specific downstream consequence. Supporting Evidence: file:mouse/Trp53/Trp53-deep-research-falcon.md The p53 transcriptional program is tissue-, stress-, and context-dependent; many annotated responses are downstream or specialized outputs rather than the core molecular activity. |
| GO:0005813 centrosome | IEA GO_REF:0000120 | KEEP AS NON CORE | Summary: centrosome is supported as a context-specific p53 output or phenotype, but it is not the core molecular function of Trp53. Reason: p53 has broad tissue- and stress-specific downstream effects; this annotation can be retained as non-core rather than treated as the primary evolved activity. Supporting Evidence: file:mouse/Trp53/Trp53-deep-research-falcon.md The p53 transcriptional program is tissue-, stress-, and context-dependent; many annotated responses are downstream or specialized outputs rather than the core molecular activity. |
| GO:0006355 regulation of DNA-templated transcription | IEA GO_REF:0000120 | ACCEPT | Summary: regulation of DNA-templated transcription is consistent with the curated mouse p53 biology as a stress-activated DNA-binding transcription factor, transcriptional regulator, or well-supported subcellular context. Reason: The term fits the direct p53 activity or a well-established cellular context for that activity, supported by UniProt and the Falcon literature synthesis. |
| GO:0006915 apoptotic process | IEA GO_REF:0000002 | KEEP AS NON CORE | Summary: apoptotic process is a broad downstream p53 cell-fate annotation rather than the core Trp53 molecular function. Reason: p53 can induce apoptotic programs after stress, but broad apoptosis terms should be retained as non-core because the core Trp53 function is sequence-specific DNA-binding transcriptional regulation. Supporting Evidence: file:mouse/Trp53/Trp53-deep-research-falcon.md Canonical apoptotic targets include Bbc3/Puma, Bax, Pmaip1/Noxa, and Apaf1. |
| GO:0006979 response to oxidative stress | IEA GO_REF:0000117 | KEEP AS NON CORE | Summary: response to oxidative stress is a supported p53-associated context, interaction, localization, or downstream output, but it is not the core Trp53 molecular function. Reason: Retain as non-core because Trp53/p53 is primarily a sequence-specific DNA-binding transcription factor; this term reflects regulatory context, stress response, localization, or a tissue-specific downstream consequence. Supporting Evidence: file:mouse/Trp53/Trp53-deep-research-falcon.md The p53 transcriptional program is tissue-, stress-, and context-dependent; many annotated responses are downstream or specialized outputs rather than the core molecular activity. |
| GO:0009410 response to xenobiotic stimulus | IEA GO_REF:0000117 | KEEP AS NON CORE | Summary: response to xenobiotic stimulus is a supported p53-associated context, interaction, localization, or downstream output, but it is not the core Trp53 molecular function. Reason: Retain as non-core because Trp53/p53 is primarily a sequence-specific DNA-binding transcription factor; this term reflects regulatory context, stress response, localization, or a tissue-specific downstream consequence. Supporting Evidence: file:mouse/Trp53/Trp53-deep-research-falcon.md The p53 transcriptional program is tissue-, stress-, and context-dependent; many annotated responses are downstream or specialized outputs rather than the core molecular activity. |
| GO:0009411 response to UV | IEA GO_REF:0000117 | KEEP AS NON CORE | Summary: response to UV is a supported p53-associated context, interaction, localization, or downstream output, but it is not the core Trp53 molecular function. Reason: Retain as non-core because Trp53/p53 is primarily a sequence-specific DNA-binding transcription factor; this term reflects regulatory context, stress response, localization, or a tissue-specific downstream consequence. Supporting Evidence: file:mouse/Trp53/Trp53-deep-research-falcon.md The p53 transcriptional program is tissue-, stress-, and context-dependent; many annotated responses are downstream or specialized outputs rather than the core molecular activity. |
| GO:0010165 response to X-ray | IEA GO_REF:0000117 | KEEP AS NON CORE | Summary: response to X-ray is a supported p53-associated context, interaction, localization, or downstream output, but it is not the core Trp53 molecular function. Reason: Retain as non-core because Trp53/p53 is primarily a sequence-specific DNA-binding transcription factor; this term reflects regulatory context, stress response, localization, or a tissue-specific downstream consequence. Supporting Evidence: file:mouse/Trp53/Trp53-deep-research-falcon.md The p53 transcriptional program is tissue-, stress-, and context-dependent; many annotated responses are downstream or specialized outputs rather than the core molecular activity. |
| GO:0016605 PML body | IEA GO_REF:0000120 | KEEP AS NON CORE | Summary: PML body is a supported p53-associated context, interaction, localization, or downstream output, but it is not the core Trp53 molecular function. Reason: Retain as non-core because Trp53/p53 is primarily a sequence-specific DNA-binding transcription factor; this term reflects regulatory context, stress response, localization, or a tissue-specific downstream consequence. Supporting Evidence: file:mouse/Trp53/Trp53-deep-research-falcon.md The p53 transcriptional program is tissue-, stress-, and context-dependent; many annotated responses are downstream or specialized outputs rather than the core molecular activity. |
| GO:0048468 cell development | IEA GO_REF:0000117 | KEEP AS NON CORE | Summary: cell development is supported as a context-specific p53 output or phenotype, but it is not the core molecular function of Trp53. Reason: p53 has broad tissue- and stress-specific downstream effects; this annotation can be retained as non-core rather than treated as the primary evolved activity. Supporting Evidence: file:mouse/Trp53/Trp53-deep-research-falcon.md The p53 transcriptional program is tissue-, stress-, and context-dependent; many annotated responses are downstream or specialized outputs rather than the core molecular activity. |
| GO:0051053 negative regulation of DNA metabolic process | IEA GO_REF:0000117 | KEEP AS NON CORE | Summary: negative regulation of DNA metabolic process is a supported p53-associated context, interaction, localization, or downstream output, but it is not the core Trp53 molecular function. Reason: Retain as non-core because Trp53/p53 is primarily a sequence-specific DNA-binding transcription factor; this term reflects regulatory context, stress response, localization, or a tissue-specific downstream consequence. Supporting Evidence: file:mouse/Trp53/Trp53-deep-research-falcon.md The p53 transcriptional program is tissue-, stress-, and context-dependent; many annotated responses are downstream or specialized outputs rather than the core molecular activity. |
| GO:0051262 protein tetramerization | IEA GO_REF:0000002 | ACCEPT | Summary: protein tetramerization is consistent with the curated mouse p53 biology as a stress-activated DNA-binding transcription factor, transcriptional regulator, or well-supported subcellular context. Reason: The term fits the direct p53 activity or a well-established cellular context for that activity, supported by UniProt and the Falcon literature synthesis. |
| GO:0097190 apoptotic signaling pathway | IEA GO_REF:0000117 | ACCEPT | Summary: apoptotic signaling pathway is consistent with the curated mouse p53 biology as a stress-activated DNA-binding transcription factor, transcriptional regulator, or well-supported subcellular context. Reason: The term fits the direct p53 activity or a well-established cellular context for that activity, supported by UniProt and the Falcon literature synthesis. |
| GO:0097371 MDM2/MDM4 family protein binding | IEA GO_REF:0000117 | KEEP AS NON CORE | Summary: MDM2/MDM4 family protein binding is a valid p53 regulatory interaction, but it belongs outside the core function set. Reason: Retain as non-core because MDM2/MDM4 binding controls p53 stability and activity, whereas the core Trp53 function is sequence-specific transcriptional regulation of stress-response targets. Supporting Evidence: file:mouse/Trp53/Trp53-uniprot.txt Interacts with MDM2; leading to ubiquitination and proteasomal degradation of TP53. file:mouse/Trp53/Trp53-deep-research-falcon.md MDM4/MDMX restrains p53 transactivation and forms a heterodimer with MDM2 to enhance p53 degradation. |
| GO:2001242 regulation of intrinsic apoptotic signaling pathway | IEA GO_REF:0000117 | ACCEPT | Summary: regulation of intrinsic apoptotic signaling pathway is consistent with the curated mouse p53 biology as a stress-activated DNA-binding transcription factor, transcriptional regulator, or well-supported subcellular context. Reason: The term fits the direct p53 activity or a well-established cellular context for that activity, supported by UniProt and the Falcon literature synthesis. |
| GO:0005515 protein binding | IPI PMID:10329544 Hepatitis C virus core protein interacts with a human DEAD b... | REMOVE | Summary: protein binding is not a useful retained annotation for mouse p53 in this review, usually because it is too generic, weakly informative, or not directly tied to the curated p53 activity. Reason: The core Trp53 function is sequence-specific transcriptional regulation of stress-response programs; this term does not add a biologically informative or direct GO assertion. Supporting Evidence: file:mouse/Trp53/Trp53-deep-research-falcon.md Mouse p53 is primarily a sequence-specific DNA-binding transcription factor; generic binding and broad phenotype terms do not capture the curated core activity. |
| GO:0005515 protein binding | IPI PMID:11181729 The AKT3 potassium channel protein interacts with the AtPP2C... | REMOVE | Summary: protein binding is not a useful retained annotation for mouse p53 in this review, usually because it is too generic, weakly informative, or not directly tied to the curated p53 activity. Reason: The core Trp53 function is sequence-specific transcriptional regulation of stress-response programs; this term does not add a biologically informative or direct GO assertion. Supporting Evidence: file:mouse/Trp53/Trp53-deep-research-falcon.md Mouse p53 is primarily a sequence-specific DNA-binding transcription factor; generic binding and broad phenotype terms do not capture the curated core activity. |
| GO:0005515 protein binding | IPI PMID:11274052 Naked cuticle targets dishevelled to antagonize Wnt signal t... | REMOVE | Summary: protein binding is not a useful retained annotation for mouse p53 in this review, usually because it is too generic, weakly informative, or not directly tied to the curated p53 activity. Reason: The core Trp53 function is sequence-specific transcriptional regulation of stress-response programs; this term does not add a biologically informative or direct GO assertion. Supporting Evidence: file:mouse/Trp53/Trp53-deep-research-falcon.md Mouse p53 is primarily a sequence-specific DNA-binding transcription factor; generic binding and broad phenotype terms do not capture the curated core activity. |
| GO:0005515 protein binding | IPI PMID:11781573 Design and application of a cytokine-receptor-based interact... | REMOVE | Summary: protein binding is not a useful retained annotation for mouse p53 in this review, usually because it is too generic, weakly informative, or not directly tied to the curated p53 activity. Reason: The core Trp53 function is sequence-specific transcriptional regulation of stress-response programs; this term does not add a biologically informative or direct GO assertion. Supporting Evidence: file:mouse/Trp53/Trp53-deep-research-falcon.md Mouse p53 is primarily a sequence-specific DNA-binding transcription factor; generic binding and broad phenotype terms do not capture the curated core activity. |
| GO:0005515 protein binding | IPI PMID:16151013 PUMA couples the nuclear and cytoplasmic proapoptotic functi... | REMOVE | Summary: protein binding is not a useful retained annotation for mouse p53 in this review, usually because it is too generic, weakly informative, or not directly tied to the curated p53 activity. Reason: The core Trp53 function is sequence-specific transcriptional regulation of stress-response programs; this term does not add a biologically informative or direct GO assertion. Supporting Evidence: file:mouse/Trp53/Trp53-deep-research-falcon.md Mouse p53 is primarily a sequence-specific DNA-binding transcription factor; generic binding and broad phenotype terms do not capture the curated core activity. |
| GO:0005515 protein binding | IPI PMID:16480949 The intracellular domain of amyloid precursor protein intera... | REMOVE | Summary: protein binding is not a useful retained annotation for mouse p53 in this review, usually because it is too generic, weakly informative, or not directly tied to the curated p53 activity. Reason: The core Trp53 function is sequence-specific transcriptional regulation of stress-response programs; this term does not add a biologically informative or direct GO assertion. Supporting Evidence: file:mouse/Trp53/Trp53-deep-research-falcon.md Mouse p53 is primarily a sequence-specific DNA-binding transcription factor; generic binding and broad phenotype terms do not capture the curated core activity. |
| GO:0005515 protein binding | IPI PMID:16697373 Interaction of nucleoside diphosphate kinase and catalases f... | REMOVE | Summary: protein binding is not a useful retained annotation for mouse p53 in this review, usually because it is too generic, weakly informative, or not directly tied to the curated p53 activity. Reason: The core Trp53 function is sequence-specific transcriptional regulation of stress-response programs; this term does not add a biologically informative or direct GO assertion. Supporting Evidence: file:mouse/Trp53/Trp53-deep-research-falcon.md Mouse p53 is primarily a sequence-specific DNA-binding transcription factor; generic binding and broad phenotype terms do not capture the curated core activity. |
| GO:0005515 protein binding | IPI PMID:17535810 Functional similarity between the chloroplast translocon com... | REMOVE | Summary: protein binding is not a useful retained annotation for mouse p53 in this review, usually because it is too generic, weakly informative, or not directly tied to the curated p53 activity. Reason: The core Trp53 function is sequence-specific transcriptional regulation of stress-response programs; this term does not add a biologically informative or direct GO assertion. Supporting Evidence: file:mouse/Trp53/Trp53-deep-research-falcon.md Mouse p53 is primarily a sequence-specific DNA-binding transcription factor; generic binding and broad phenotype terms do not capture the curated core activity. |
| GO:0005515 protein binding | IPI PMID:17936560 Targeted inactivation of Mdm2 RING finger E3 ubiquitin ligas... | REMOVE | Summary: protein binding is not a useful retained annotation for mouse p53 in this review, usually because it is too generic, weakly informative, or not directly tied to the curated p53 activity. Reason: The core Trp53 function is sequence-specific transcriptional regulation of stress-response programs; this term does not add a biologically informative or direct GO assertion. Supporting Evidence: file:mouse/Trp53/Trp53-deep-research-falcon.md Mouse p53 is primarily a sequence-specific DNA-binding transcription factor; generic binding and broad phenotype terms do not capture the curated core activity. |
| GO:0005515 protein binding | IPI PMID:18025262 Interaction between transcription factor, basal transcriptio... | REMOVE | Summary: protein binding is not a useful retained annotation for mouse p53 in this review, usually because it is too generic, weakly informative, or not directly tied to the curated p53 activity. Reason: The core Trp53 function is sequence-specific transcriptional regulation of stress-response programs; this term does not add a biologically informative or direct GO assertion. Supporting Evidence: file:mouse/Trp53/Trp53-deep-research-falcon.md Mouse p53 is primarily a sequence-specific DNA-binding transcription factor; generic binding and broad phenotype terms do not capture the curated core activity. |
| GO:0005515 protein binding | IPI PMID:18303029 BAF60a interacts with p53 to recruit the SWI/SNF complex. | REMOVE | Summary: protein binding is not a useful retained annotation for mouse p53 in this review, usually because it is too generic, weakly informative, or not directly tied to the curated p53 activity. Reason: The core Trp53 function is sequence-specific transcriptional regulation of stress-response programs; this term does not add a biologically informative or direct GO assertion. Supporting Evidence: file:mouse/Trp53/Trp53-deep-research-falcon.md Mouse p53 is primarily a sequence-specific DNA-binding transcription factor; generic binding and broad phenotype terms do not capture the curated core activity. |
| GO:0005515 protein binding | IPI PMID:18359851 Hedgehog signaling overrides p53-mediated tumor suppression ... | REMOVE | Summary: protein binding is not a useful retained annotation for mouse p53 in this review, usually because it is too generic, weakly informative, or not directly tied to the curated p53 activity. Reason: The core Trp53 function is sequence-specific transcriptional regulation of stress-response programs; this term does not add a biologically informative or direct GO assertion. Supporting Evidence: file:mouse/Trp53/Trp53-deep-research-falcon.md Mouse p53 is primarily a sequence-specific DNA-binding transcription factor; generic binding and broad phenotype terms do not capture the curated core activity. |
| GO:0005515 protein binding | IPI PMID:18448518 The nucleocapsid protein of severe acute respiratory syndrom... | REMOVE | Summary: protein binding is not a useful retained annotation for mouse p53 in this review, usually because it is too generic, weakly informative, or not directly tied to the curated p53 activity. Reason: The core Trp53 function is sequence-specific transcriptional regulation of stress-response programs; this term does not add a biologically informative or direct GO assertion. Supporting Evidence: file:mouse/Trp53/Trp53-deep-research-falcon.md Mouse p53 is primarily a sequence-specific DNA-binding transcription factor; generic binding and broad phenotype terms do not capture the curated core activity. |
| GO:0005515 protein binding | IPI PMID:18695251 AIMP2/p38, the scaffold for the multi-tRNA synthetase comple... | REMOVE | Summary: protein binding is not a useful retained annotation for mouse p53 in this review, usually because it is too generic, weakly informative, or not directly tied to the curated p53 activity. Reason: The core Trp53 function is sequence-specific transcriptional regulation of stress-response programs; this term does not add a biologically informative or direct GO assertion. Supporting Evidence: file:mouse/Trp53/Trp53-deep-research-falcon.md Mouse p53 is primarily a sequence-specific DNA-binding transcription factor; generic binding and broad phenotype terms do not capture the curated core activity. |
| GO:0005515 protein binding | IPI PMID:19011633 Bach1 inhibits oxidative stress-induced cellular senescence ... | REMOVE | Summary: protein binding is not a useful retained annotation for mouse p53 in this review, usually because it is too generic, weakly informative, or not directly tied to the curated p53 activity. Reason: The core Trp53 function is sequence-specific transcriptional regulation of stress-response programs; this term does not add a biologically informative or direct GO assertion. Supporting Evidence: file:mouse/Trp53/Trp53-deep-research-falcon.md Mouse p53 is primarily a sequence-specific DNA-binding transcription factor; generic binding and broad phenotype terms do not capture the curated core activity. |
| GO:0005515 protein binding | IPI PMID:19556538 Trim24 targets endogenous p53 for degradation. | REMOVE | Summary: protein binding is not a useful retained annotation for mouse p53 in this review, usually because it is too generic, weakly informative, or not directly tied to the curated p53 activity. Reason: The core Trp53 function is sequence-specific transcriptional regulation of stress-response programs; this term does not add a biologically informative or direct GO assertion. Supporting Evidence: file:mouse/Trp53/Trp53-deep-research-falcon.md Mouse p53 is primarily a sequence-specific DNA-binding transcription factor; generic binding and broad phenotype terms do not capture the curated core activity. |
| GO:0005515 protein binding | IPI PMID:20621096 Deletion of Swm2p selectively impairs trimethylation of snRN... | REMOVE | Summary: protein binding is not a useful retained annotation for mouse p53 in this review, usually because it is too generic, weakly informative, or not directly tied to the curated p53 activity. Reason: The core Trp53 function is sequence-specific transcriptional regulation of stress-response programs; this term does not add a biologically informative or direct GO assertion. Supporting Evidence: file:mouse/Trp53/Trp53-deep-research-falcon.md Mouse p53 is primarily a sequence-specific DNA-binding transcription factor; generic binding and broad phenotype terms do not capture the curated core activity. |
| GO:0005515 protein binding | IPI PMID:20697359 Regulation of MDM4 (MDMX) function by p76(MDM2): a new facet... | REMOVE | Summary: protein binding is not a useful retained annotation for mouse p53 in this review, usually because it is too generic, weakly informative, or not directly tied to the curated p53 activity. Reason: The core Trp53 function is sequence-specific transcriptional regulation of stress-response programs; this term does not add a biologically informative or direct GO assertion. Supporting Evidence: file:mouse/Trp53/Trp53-deep-research-falcon.md Mouse p53 is primarily a sequence-specific DNA-binding transcription factor; generic binding and broad phenotype terms do not capture the curated core activity. |
| GO:0005515 protein binding | IPI PMID:20708612 DJ-1, an oncogene and causative gene for familial Parkinson'... | REMOVE | Summary: protein binding is not a useful retained annotation for mouse p53 in this review, usually because it is too generic, weakly informative, or not directly tied to the curated p53 activity. Reason: The core Trp53 function is sequence-specific transcriptional regulation of stress-response programs; this term does not add a biologically informative or direct GO assertion. Supporting Evidence: file:mouse/Trp53/Trp53-deep-research-falcon.md Mouse p53 is primarily a sequence-specific DNA-binding transcription factor; generic binding and broad phenotype terms do not capture the curated core activity. |
| GO:0005515 protein binding | IPI PMID:20832751 An ARF-independent c-MYC-activated tumor suppression pathway... | REMOVE | Summary: protein binding is not a useful retained annotation for mouse p53 in this review, usually because it is too generic, weakly informative, or not directly tied to the curated p53 activity. Reason: The core Trp53 function is sequence-specific transcriptional regulation of stress-response programs; this term does not add a biologically informative or direct GO assertion. Supporting Evidence: file:mouse/Trp53/Trp53-deep-research-falcon.md Mouse p53 is primarily a sequence-specific DNA-binding transcription factor; generic binding and broad phenotype terms do not capture the curated core activity. |
| GO:0005515 protein binding | IPI PMID:21057544 p85Ξ± mediates p53 K370 acetylation by p300 and regulates its... | REMOVE | Summary: protein binding is not a useful retained annotation for mouse p53 in this review, usually because it is too generic, weakly informative, or not directly tied to the curated p53 activity. Reason: The core Trp53 function is sequence-specific transcriptional regulation of stress-response programs; this term does not add a biologically informative or direct GO assertion. Supporting Evidence: file:mouse/Trp53/Trp53-deep-research-falcon.md Mouse p53 is primarily a sequence-specific DNA-binding transcription factor; generic binding and broad phenotype terms do not capture the curated core activity. |
| GO:0005515 protein binding | IPI PMID:21122074 Interaction of Sesbania mosaic virus movement protein with t... | REMOVE | Summary: protein binding is not a useful retained annotation for mouse p53 in this review, usually because it is too generic, weakly informative, or not directly tied to the curated p53 activity. Reason: The core Trp53 function is sequence-specific transcriptional regulation of stress-response programs; this term does not add a biologically informative or direct GO assertion. Supporting Evidence: file:mouse/Trp53/Trp53-deep-research-falcon.md Mouse p53 is primarily a sequence-specific DNA-binding transcription factor; generic binding and broad phenotype terms do not capture the curated core activity. |
| GO:0005515 protein binding | IPI PMID:21670284 Near-UV cyanobacteriochrome signaling system elicits negativ... | REMOVE | Summary: protein binding is not a useful retained annotation for mouse p53 in this review, usually because it is too generic, weakly informative, or not directly tied to the curated p53 activity. Reason: The core Trp53 function is sequence-specific transcriptional regulation of stress-response programs; this term does not add a biologically informative or direct GO assertion. Supporting Evidence: file:mouse/Trp53/Trp53-deep-research-falcon.md Mouse p53 is primarily a sequence-specific DNA-binding transcription factor; generic binding and broad phenotype terms do not capture the curated core activity. |
| GO:0005515 protein binding | IPI PMID:21741598 A Pin1/mutant p53 axis promotes aggressiveness in breast can... | REMOVE | Summary: protein binding is not a useful retained annotation for mouse p53 in this review, usually because it is too generic, weakly informative, or not directly tied to the curated p53 activity. Reason: The core Trp53 function is sequence-specific transcriptional regulation of stress-response programs; this term does not add a biologically informative or direct GO assertion. Supporting Evidence: file:mouse/Trp53/Trp53-deep-research-falcon.md Mouse p53 is primarily a sequence-specific DNA-binding transcription factor; generic binding and broad phenotype terms do not capture the curated core activity. |
| GO:0005515 protein binding | IPI PMID:21857681 A stress response pathway regulates DNA damage through Ξ²2-ad... | REMOVE | Summary: protein binding is not a useful retained annotation for mouse p53 in this review, usually because it is too generic, weakly informative, or not directly tied to the curated p53 activity. Reason: The core Trp53 function is sequence-specific transcriptional regulation of stress-response programs; this term does not add a biologically informative or direct GO assertion. Supporting Evidence: file:mouse/Trp53/Trp53-deep-research-falcon.md Mouse p53 is primarily a sequence-specific DNA-binding transcription factor; generic binding and broad phenotype terms do not capture the curated core activity. |
| GO:0005515 protein binding | IPI PMID:21909133 Manganese superoxide dismutase is a mitochondrial fidelity p... | REMOVE | Summary: protein binding is not a useful retained annotation for mouse p53 in this review, usually because it is too generic, weakly informative, or not directly tied to the curated p53 activity. Reason: The core Trp53 function is sequence-specific transcriptional regulation of stress-response programs; this term does not add a biologically informative or direct GO assertion. Supporting Evidence: file:mouse/Trp53/Trp53-deep-research-falcon.md Mouse p53 is primarily a sequence-specific DNA-binding transcription factor; generic binding and broad phenotype terms do not capture the curated core activity. |
| GO:0005515 protein binding | IPI PMID:22056770 Structural basis for the molecular evolution of SRP-GTPase a... | REMOVE | Summary: protein binding is not a useful retained annotation for mouse p53 in this review, usually because it is too generic, weakly informative, or not directly tied to the curated p53 activity. Reason: The core Trp53 function is sequence-specific transcriptional regulation of stress-response programs; this term does not add a biologically informative or direct GO assertion. Supporting Evidence: file:mouse/Trp53/Trp53-deep-research-falcon.md Mouse p53 is primarily a sequence-specific DNA-binding transcription factor; generic binding and broad phenotype terms do not capture the curated core activity. |
| GO:0005515 protein binding | IPI PMID:22726440 p53 opens the mitochondrial permeability transition pore to ... | REMOVE | Summary: protein binding is not a useful retained annotation for mouse p53 in this review, usually because it is too generic, weakly informative, or not directly tied to the curated p53 activity. Reason: The core Trp53 function is sequence-specific transcriptional regulation of stress-response programs; this term does not add a biologically informative or direct GO assertion. Supporting Evidence: file:mouse/Trp53/Trp53-deep-research-falcon.md Mouse p53 is primarily a sequence-specific DNA-binding transcription factor; generic binding and broad phenotype terms do not capture the curated core activity. |
| GO:0005515 protein binding | IPI PMID:23123091 Involvement of PTEN in TPA-mediated p53-activation in mouse ... | REMOVE | Summary: protein binding is not a useful retained annotation for mouse p53 in this review, usually because it is too generic, weakly informative, or not directly tied to the curated p53 activity. Reason: The core Trp53 function is sequence-specific transcriptional regulation of stress-response programs; this term does not add a biologically informative or direct GO assertion. Supporting Evidence: file:mouse/Trp53/Trp53-deep-research-falcon.md Mouse p53 is primarily a sequence-specific DNA-binding transcription factor; generic binding and broad phenotype terms do not capture the curated core activity. |
| GO:0005515 protein binding | IPI PMID:23980194 E258K HCM-causing mutation in cardiac MyBP-C reduces contrac... | REMOVE | Summary: protein binding is not a useful retained annotation for mouse p53 in this review, usually because it is too generic, weakly informative, or not directly tied to the curated p53 activity. Reason: The core Trp53 function is sequence-specific transcriptional regulation of stress-response programs; this term does not add a biologically informative or direct GO assertion. Supporting Evidence: file:mouse/Trp53/Trp53-deep-research-falcon.md Mouse p53 is primarily a sequence-specific DNA-binding transcription factor; generic binding and broad phenotype terms do not capture the curated core activity. |
| GO:0005515 protein binding | IPI PMID:24116158 The nuclear envelope protein, LAP1B, is a novel protein phos... | REMOVE | Summary: protein binding is not a useful retained annotation for mouse p53 in this review, usually because it is too generic, weakly informative, or not directly tied to the curated p53 activity. Reason: The core Trp53 function is sequence-specific transcriptional regulation of stress-response programs; this term does not add a biologically informative or direct GO assertion. Supporting Evidence: file:mouse/Trp53/Trp53-deep-research-falcon.md Mouse p53 is primarily a sequence-specific DNA-binding transcription factor; generic binding and broad phenotype terms do not capture the curated core activity. |
| GO:0005515 protein binding | IPI PMID:25117711 Regulation of p53 by Mdm2 E3 ligase function is dispensable ... | REMOVE | Summary: protein binding is not a useful retained annotation for mouse p53 in this review, usually because it is too generic, weakly informative, or not directly tied to the curated p53 activity. Reason: The core Trp53 function is sequence-specific transcriptional regulation of stress-response programs; this term does not add a biologically informative or direct GO assertion. Supporting Evidence: file:mouse/Trp53/Trp53-deep-research-falcon.md Mouse p53 is primarily a sequence-specific DNA-binding transcription factor; generic binding and broad phenotype terms do not capture the curated core activity. |
| GO:0005515 protein binding | IPI PMID:26960425 Yeast Two-Hybrid Screening for Proteins that Interact with t... | REMOVE | Summary: protein binding is not a useful retained annotation for mouse p53 in this review, usually because it is too generic, weakly informative, or not directly tied to the curated p53 activity. Reason: The core Trp53 function is sequence-specific transcriptional regulation of stress-response programs; this term does not add a biologically informative or direct GO assertion. Supporting Evidence: file:mouse/Trp53/Trp53-deep-research-falcon.md Mouse p53 is primarily a sequence-specific DNA-binding transcription factor; generic binding and broad phenotype terms do not capture the curated core activity. |
| GO:0005515 protein binding | IPI PMID:28536627 The identification of human aldo-keto reductase AKR7A2 as a ... | REMOVE | Summary: protein binding is not a useful retained annotation for mouse p53 in this review, usually because it is too generic, weakly informative, or not directly tied to the curated p53 activity. Reason: The core Trp53 function is sequence-specific transcriptional regulation of stress-response programs; this term does not add a biologically informative or direct GO assertion. Supporting Evidence: file:mouse/Trp53/Trp53-deep-research-falcon.md Mouse p53 is primarily a sequence-specific DNA-binding transcription factor; generic binding and broad phenotype terms do not capture the curated core activity. |
| GO:0005515 protein binding | IPI PMID:37025149 Arabidopsis LFR, a SWI/SNF complex component, interacts with... | REMOVE | Summary: protein binding is not a useful retained annotation for mouse p53 in this review, usually because it is too generic, weakly informative, or not directly tied to the curated p53 activity. Reason: The core Trp53 function is sequence-specific transcriptional regulation of stress-response programs; this term does not add a biologically informative or direct GO assertion. Supporting Evidence: file:mouse/Trp53/Trp53-deep-research-falcon.md Mouse p53 is primarily a sequence-specific DNA-binding transcription factor; generic binding and broad phenotype terms do not capture the curated core activity. |
| GO:0005515 protein binding | IPI PMID:9194565 Binding and modulation of p53 by p300/CBP coactivators. | REMOVE | Summary: protein binding is not a useful retained annotation for mouse p53 in this review, usually because it is too generic, weakly informative, or not directly tied to the curated p53 activity. Reason: The core Trp53 function is sequence-specific transcriptional regulation of stress-response programs; this term does not add a biologically informative or direct GO assertion. Supporting Evidence: file:mouse/Trp53/Trp53-deep-research-falcon.md Mouse p53 is primarily a sequence-specific DNA-binding transcription factor; generic binding and broad phenotype terms do not capture the curated core activity. |
| GO:0000122 negative regulation of transcription by RNA polymerase II | IEA GO_REF:0000107 | ACCEPT | Summary: negative regulation of transcription by RNA polymerase II is consistent with the curated mouse p53 biology as a stress-activated DNA-binding transcription factor, transcriptional regulator, or well-supported subcellular context. Reason: The term fits the direct p53 activity or a well-established cellular context for that activity, supported by UniProt and the Falcon literature synthesis. |
| GO:0000785 chromatin | IEA GO_REF:0000107 | ACCEPT | Summary: chromatin is consistent with the curated mouse p53 biology as a stress-activated DNA-binding transcription factor, transcriptional regulator, or well-supported subcellular context. Reason: The term fits the direct p53 activity or a well-established cellular context for that activity, supported by UniProt and the Falcon literature synthesis. |
| GO:0000978 RNA polymerase II cis-regulatory region sequence-specific DNA binding | IEA GO_REF:0000107 | ACCEPT | Summary: RNA polymerase II cis-regulatory region sequence-specific DNA binding is consistent with the curated mouse p53 biology as a stress-activated DNA-binding transcription factor, transcriptional regulator, or well-supported subcellular context. Reason: The term fits the direct p53 activity or a well-established cellular context for that activity, supported by UniProt and the Falcon literature synthesis. |
| GO:0000981 DNA-binding transcription factor activity, RNA polymerase II-specific | IEA GO_REF:0000107 | ACCEPT | Summary: DNA-binding transcription factor activity, RNA polymerase II-specific is consistent with the curated mouse p53 biology as a stress-activated DNA-binding transcription factor, transcriptional regulator, or well-supported subcellular context. Reason: The term fits the direct p53 activity or a well-established cellular context for that activity, supported by UniProt and the Falcon literature synthesis. |
| GO:0000987 cis-regulatory region sequence-specific DNA binding | IEA GO_REF:0000107 | ACCEPT | Summary: cis-regulatory region sequence-specific DNA binding is consistent with the curated mouse p53 biology as a stress-activated DNA-binding transcription factor, transcriptional regulator, or well-supported subcellular context. Reason: The term fits the direct p53 activity or a well-established cellular context for that activity, supported by UniProt and the Falcon literature synthesis. |
| GO:0001046 core promoter sequence-specific DNA binding | IEA GO_REF:0000107 | ACCEPT | Summary: core promoter sequence-specific DNA binding is consistent with the curated mouse p53 biology as a stress-activated DNA-binding transcription factor, transcriptional regulator, or well-supported subcellular context. Reason: The term fits the direct p53 activity or a well-established cellular context for that activity, supported by UniProt and the Falcon literature synthesis. |
| GO:0001094 TFIID-class transcription factor complex binding | IEA GO_REF:0000107 | KEEP AS NON CORE | Summary: TFIID-class transcription factor complex binding is a documented p53 regulatory interaction, but not the core Trp53 molecular function. Reason: Retain as non-core because p53 is primarily curated here as a sequence-specific DNA-binding transcription factor; TFIID/TAF binding is a cofactor interaction that modulates transcriptional regulation. Supporting Evidence: file:mouse/Trp53/Trp53-uniprot.txt Interacts (via C-terminus) with TAF1; when TAF1 is part of the TFIID complex. |
| GO:0001223 transcription coactivator binding | IEA GO_REF:0000107 | KEEP AS NON CORE | Summary: transcription coactivator binding is a supported p53-associated context, interaction, localization, or downstream output, but it is not the core Trp53 molecular function. Reason: Retain as non-core because Trp53/p53 is primarily a sequence-specific DNA-binding transcription factor; this term reflects regulatory context, stress response, localization, or a tissue-specific downstream consequence. Supporting Evidence: file:mouse/Trp53/Trp53-deep-research-falcon.md The p53 transcriptional program is tissue-, stress-, and context-dependent; many annotated responses are downstream or specialized outputs rather than the core molecular activity. |
| GO:0001227 DNA-binding transcription repressor activity, RNA polymerase II-specific | IEA GO_REF:0000107 | ACCEPT | Summary: DNA-binding transcription repressor activity, RNA polymerase II-specific is consistent with the curated mouse p53 biology as a stress-activated DNA-binding transcription factor, transcriptional regulator, or well-supported subcellular context. Reason: The term fits the direct p53 activity or a well-established cellular context for that activity, supported by UniProt and the Falcon literature synthesis. |
| GO:0001228 DNA-binding transcription activator activity, RNA polymerase II-specific | IEA GO_REF:0000107 | ACCEPT | Summary: DNA-binding transcription activator activity, RNA polymerase II-specific is consistent with the curated mouse p53 biology as a stress-activated DNA-binding transcription factor, transcriptional regulator, or well-supported subcellular context. Reason: The term fits the direct p53 activity or a well-established cellular context for that activity, supported by UniProt and the Falcon literature synthesis. |
| GO:0002020 protease binding | IEA GO_REF:0000107 | KEEP AS NON CORE | Summary: protease binding is a supported p53-associated context, interaction, localization, or downstream output, but it is not the core Trp53 molecular function. Reason: Retain as non-core because Trp53/p53 is primarily a sequence-specific DNA-binding transcription factor; this term reflects regulatory context, stress response, localization, or a tissue-specific downstream consequence. Supporting Evidence: file:mouse/Trp53/Trp53-deep-research-falcon.md The p53 transcriptional program is tissue-, stress-, and context-dependent; many annotated responses are downstream or specialized outputs rather than the core molecular activity. |
| GO:0002039 p53 binding | IEA GO_REF:0000107 | KEEP AS NON CORE | Summary: p53 binding is a supported p53-associated context, interaction, localization, or downstream output, but it is not the core Trp53 molecular function. Reason: Retain as non-core because Trp53/p53 is primarily a sequence-specific DNA-binding transcription factor; this term reflects regulatory context, stress response, localization, or a tissue-specific downstream consequence. Supporting Evidence: file:mouse/Trp53/Trp53-deep-research-falcon.md The p53 transcriptional program is tissue-, stress-, and context-dependent; many annotated responses are downstream or specialized outputs rather than the core molecular activity. |
| GO:0002244 hematopoietic progenitor cell differentiation | IEA GO_REF:0000107 | KEEP AS NON CORE | Summary: hematopoietic progenitor cell differentiation is supported as a context-specific p53 output or phenotype, but it is not the core molecular function of Trp53. Reason: p53 has broad tissue- and stress-specific downstream effects; this annotation can be retained as non-core rather than treated as the primary evolved activity. Supporting Evidence: file:mouse/Trp53/Trp53-deep-research-falcon.md The p53 transcriptional program is tissue-, stress-, and context-dependent; many annotated responses are downstream or specialized outputs rather than the core molecular activity. |
| GO:0003682 chromatin binding | IEA GO_REF:0000107 | ACCEPT | Summary: chromatin binding is consistent with the curated mouse p53 biology as a stress-activated DNA-binding transcription factor, transcriptional regulator, or well-supported subcellular context. Reason: The term fits the direct p53 activity or a well-established cellular context for that activity, supported by UniProt and the Falcon literature synthesis. |
| GO:0003730 mRNA 3'-UTR binding | IEA GO_REF:0000107 | KEEP AS NON CORE | Summary: mRNA 3'-UTR binding is a supported p53-associated context, interaction, localization, or downstream output, but it is not the core Trp53 molecular function. Reason: Retain as non-core because Trp53/p53 is primarily a sequence-specific DNA-binding transcription factor; this term reflects regulatory context, stress response, localization, or a tissue-specific downstream consequence. Supporting Evidence: file:mouse/Trp53/Trp53-deep-research-falcon.md The p53 transcriptional program is tissue-, stress-, and context-dependent; many annotated responses are downstream or specialized outputs rather than the core molecular activity. |
| GO:0005507 copper ion binding | IEA GO_REF:0000107 | MODIFY | Summary: Copper ion binding is not supported for mouse p53 by the cited evidence; UniProt documents Zn(2+) binding instead. Reason: Replace the unsupported copper-binding term with GO:0008270 zinc ion binding, the metal cofactor documented for p53. Proposed replacements: zinc ion binding Supporting Evidence: file:mouse/Trp53/Trp53-uniprot.txt Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Note=Binds 1 zinc ion per subunit. |
| GO:0005654 nucleoplasm | IEA GO_REF:0000120 | ACCEPT | Summary: nucleoplasm is consistent with the curated mouse p53 biology as a stress-activated DNA-binding transcription factor, transcriptional regulator, or well-supported subcellular context. Reason: The term fits the direct p53 activity or a well-established cellular context for that activity, supported by UniProt and the Falcon literature synthesis. |
| GO:0005667 transcription regulator complex | IEA GO_REF:0000107 | ACCEPT | Summary: transcription regulator complex is consistent with the curated mouse p53 biology as a stress-activated DNA-binding transcription factor, transcriptional regulator, or well-supported subcellular context. Reason: The term fits the direct p53 activity or a well-established cellular context for that activity, supported by UniProt and the Falcon literature synthesis. |
| GO:0005730 nucleolus | IEA GO_REF:0000107 | KEEP AS NON CORE | Summary: nucleolus is a supported p53-associated context, interaction, localization, or downstream output, but it is not the core Trp53 molecular function. Reason: Retain as non-core because Trp53/p53 is primarily a sequence-specific DNA-binding transcription factor; this term reflects regulatory context, stress response, localization, or a tissue-specific downstream consequence. Supporting Evidence: file:mouse/Trp53/Trp53-deep-research-falcon.md The p53 transcriptional program is tissue-, stress-, and context-dependent; many annotated responses are downstream or specialized outputs rather than the core molecular activity. |
| GO:0005739 mitochondrion | IEA GO_REF:0000107 | KEEP AS NON CORE | Summary: mitochondrion is a supported p53-associated context, interaction, localization, or downstream output, but it is not the core Trp53 molecular function. Reason: Retain as non-core because Trp53/p53 is primarily a sequence-specific DNA-binding transcription factor; this term reflects regulatory context, stress response, localization, or a tissue-specific downstream consequence. Supporting Evidence: file:mouse/Trp53/Trp53-deep-research-falcon.md The p53 transcriptional program is tissue-, stress-, and context-dependent; many annotated responses are downstream or specialized outputs rather than the core molecular activity. |
| GO:0005829 cytosol | IEA GO_REF:0000120 | KEEP AS NON CORE | Summary: cytosol is a supported p53-associated context, interaction, localization, or downstream output, but it is not the core Trp53 molecular function. Reason: Retain as non-core because Trp53/p53 is primarily a sequence-specific DNA-binding transcription factor; this term reflects regulatory context, stress response, localization, or a tissue-specific downstream consequence. Supporting Evidence: file:mouse/Trp53/Trp53-deep-research-falcon.md The p53 transcriptional program is tissue-, stress-, and context-dependent; many annotated responses are downstream or specialized outputs rather than the core molecular activity. |
| GO:0006289 nucleotide-excision repair | IEA GO_REF:0000107 | ACCEPT | Summary: nucleotide-excision repair is consistent with the curated mouse p53 biology as a stress-activated DNA-binding transcription factor, transcriptional regulator, or well-supported subcellular context. Reason: The term fits the direct p53 activity or a well-established cellular context for that activity, supported by UniProt and the Falcon literature synthesis. |
| GO:0006357 regulation of transcription by RNA polymerase II | IEA GO_REF:0000107 | ACCEPT | Summary: regulation of transcription by RNA polymerase II is consistent with the curated mouse p53 biology as a stress-activated DNA-binding transcription factor, transcriptional regulator, or well-supported subcellular context. Reason: The term fits the direct p53 activity or a well-established cellular context for that activity, supported by UniProt and the Falcon literature synthesis. |
| GO:0006914 autophagy | IEA GO_REF:0000107 | KEEP AS NON CORE | Summary: autophagy is a supported p53-associated context, interaction, localization, or downstream output, but it is not the core Trp53 molecular function. Reason: Retain as non-core because Trp53/p53 is primarily a sequence-specific DNA-binding transcription factor; this term reflects regulatory context, stress response, localization, or a tissue-specific downstream consequence. Supporting Evidence: file:mouse/Trp53/Trp53-deep-research-falcon.md The p53 transcriptional program is tissue-, stress-, and context-dependent; many annotated responses are downstream or specialized outputs rather than the core molecular activity. |
| GO:0007265 Ras protein signal transduction | IEA GO_REF:0000107 | MARK AS OVER ANNOTATED | Summary: Ras protein signal transduction is not the direct molecular activity of p53. It may reflect downstream pathway crosstalk from stress and proliferation programs, but it overstates the core p53 function. Reason: p53 is a sequence-specific transcription factor and stress-response regulator. A broad Ras signaling annotation is too pathway-level and indirect for the available evidence. Supporting Evidence: file:mouse/Trp53/Trp53-deep-research-falcon.md Mouse p53 is primarily a sequence-specific DNA-binding transcription factor; generic binding and broad phenotype terms do not capture the curated core activity. |
| GO:0008104 intracellular protein localization | IEA GO_REF:0000107 | MARK AS OVER ANNOTATED | Summary: Intracellular protein localization is an inferred broad process annotation. p53 itself is regulated by MDM2-mediated nuclear export and stress-dependent nuclear accumulation, but this does not make protein-localization control a core p53 output. Reason: The evidence supports p53 subcellular regulation, not a direct core role for p53 in intracellular protein localization. Supporting Evidence: file:mouse/Trp53/Trp53-deep-research-falcon.md Functionally, p53 acts predominantly in the nucleus as a transcription factor. Consistent with this, MDM2-mediated regulation explicitly includes export of nuclear p53 to the cytoplasm, and stress-dependent disruption of this repression allows nuclear accumulation and transcriptional activation. file:mouse/Trp53/Trp53-uniprot.txt GO; GO:0008104; P:intracellular protein localization; ISO:GO_Central. |
| GO:0008285 negative regulation of cell population proliferation | IEA GO_REF:0000107 | ACCEPT | Summary: negative regulation of cell population proliferation is consistent with the curated mouse p53 biology as a stress-activated DNA-binding transcription factor, transcriptional regulator, or well-supported subcellular context. Reason: The term fits the direct p53 activity or a well-established cellular context for that activity, supported by UniProt and the Falcon literature synthesis. |
| GO:0009299 mRNA transcription | IEA GO_REF:0000107 | KEEP AS NON CORE | Summary: mRNA transcription is supported as a context-specific p53 output or phenotype, but it is not the core molecular function of Trp53. Reason: p53 has broad tissue- and stress-specific downstream effects; this annotation can be retained as non-core rather than treated as the primary evolved activity. Supporting Evidence: file:mouse/Trp53/Trp53-deep-research-falcon.md The p53 transcriptional program is tissue-, stress-, and context-dependent; many annotated responses are downstream or specialized outputs rather than the core molecular activity. |
| GO:0010332 response to gamma radiation | IEA GO_REF:0000107 | KEEP AS NON CORE | Summary: response to gamma radiation is a supported p53-associated context, interaction, localization, or downstream output, but it is not the core Trp53 molecular function. Reason: Retain as non-core because Trp53/p53 is primarily a sequence-specific DNA-binding transcription factor; this term reflects regulatory context, stress response, localization, or a tissue-specific downstream consequence. Supporting Evidence: file:mouse/Trp53/Trp53-deep-research-falcon.md The p53 transcriptional program is tissue-, stress-, and context-dependent; many annotated responses are downstream or specialized outputs rather than the core molecular activity. |
| GO:0010628 positive regulation of gene expression | IEA GO_REF:0000107 | ACCEPT | Summary: positive regulation of gene expression is consistent with the curated mouse p53 biology as a stress-activated DNA-binding transcription factor, transcriptional regulator, or well-supported subcellular context. Reason: The term fits the direct p53 activity or a well-established cellular context for that activity, supported by UniProt and the Falcon literature synthesis. |
| GO:0016032 viral process | IEA GO_REF:0000107 | KEEP AS NON CORE | Summary: viral process is supported as a context-specific p53 output or phenotype, but it is not the core molecular function of Trp53. Reason: p53 has broad tissue- and stress-specific downstream effects; this annotation can be retained as non-core rather than treated as the primary evolved activity. Supporting Evidence: file:mouse/Trp53/Trp53-deep-research-falcon.md The p53 transcriptional program is tissue-, stress-, and context-dependent; many annotated responses are downstream or specialized outputs rather than the core molecular activity. |
| GO:0016363 nuclear matrix | IEA GO_REF:0000107 | KEEP AS NON CORE | Summary: nuclear matrix is a supported p53-associated context, interaction, localization, or downstream output, but it is not the core Trp53 molecular function. Reason: Retain as non-core because Trp53/p53 is primarily a sequence-specific DNA-binding transcription factor; this term reflects regulatory context, stress response, localization, or a tissue-specific downstream consequence. Supporting Evidence: file:mouse/Trp53/Trp53-deep-research-falcon.md The p53 transcriptional program is tissue-, stress-, and context-dependent; many annotated responses are downstream or specialized outputs rather than the core molecular activity. |
| GO:0016604 nuclear body | IEA GO_REF:0000107 | KEEP AS NON CORE | Summary: nuclear body is a supported p53-associated context, interaction, localization, or downstream output, but it is not the core Trp53 molecular function. Reason: Retain as non-core because Trp53/p53 is primarily a sequence-specific DNA-binding transcription factor; this term reflects regulatory context, stress response, localization, or a tissue-specific downstream consequence. Supporting Evidence: file:mouse/Trp53/Trp53-deep-research-falcon.md The p53 transcriptional program is tissue-, stress-, and context-dependent; many annotated responses are downstream or specialized outputs rather than the core molecular activity. |
| GO:0019899 enzyme binding | IEA GO_REF:0000107 | REMOVE | Summary: enzyme binding is not a useful retained annotation for mouse p53 in this review, usually because it is too generic, weakly informative, or not directly tied to the curated p53 activity. Reason: The core Trp53 function is sequence-specific transcriptional regulation of stress-response programs; this term does not add a biologically informative or direct GO assertion. Supporting Evidence: file:mouse/Trp53/Trp53-deep-research-falcon.md Mouse p53 is primarily a sequence-specific DNA-binding transcription factor; generic binding and broad phenotype terms do not capture the curated core activity. |
| GO:0030308 negative regulation of cell growth | IEA GO_REF:0000107 | ACCEPT | Summary: negative regulation of cell growth is consistent with the curated mouse p53 biology as a stress-activated DNA-binding transcription factor, transcriptional regulator, or well-supported subcellular context. Reason: The term fits the direct p53 activity or a well-established cellular context for that activity, supported by UniProt and the Falcon literature synthesis. |
| GO:0030330 DNA damage response, signal transduction by p53 class mediator | IEA GO_REF:0000107 | ACCEPT | Summary: DNA damage response, signal transduction by p53 class mediator is consistent with the curated mouse p53 biology as a stress-activated DNA-binding transcription factor, transcriptional regulator, or well-supported subcellular context. Reason: The term fits the direct p53 activity or a well-established cellular context for that activity, supported by UniProt and the Falcon literature synthesis. |
| GO:0030971 receptor tyrosine kinase binding | IEA GO_REF:0000107 | REMOVE | Summary: Receptor tyrosine kinase binding is not supported as a direct retained p53 molecular function in the reviewed evidence. UniProt notes many p53 regulatory interactions, including non-receptor PTK2/PTK2B contexts, but not a well-supported receptor tyrosine kinase binding activity. Reason: The annotation appears to be an over-propagated generic binding claim. It is not justified by the Falcon synthesis or UniProt evidence for p53's core DNA-binding transcription factor activity. Supporting Evidence: file:mouse/Trp53/Trp53-deep-research-falcon.md Mouse p53 is primarily a sequence-specific DNA-binding transcription factor; generic binding and broad phenotype terms do not capture the curated core activity. file:mouse/Trp53/Trp53-uniprot.txt Interacts (via N-terminus) with PTK2/FAK1; this promotes ubiquitination by MDM2. Interacts with PTK2B/PYK2. |
| GO:0031571 mitotic G1 DNA damage checkpoint signaling | IEA GO_REF:0000107 | ACCEPT | Summary: mitotic G1 DNA damage checkpoint signaling is consistent with the curated mouse p53 biology as a stress-activated DNA-binding transcription factor, transcriptional regulator, or well-supported subcellular context. Reason: The term fits the direct p53 activity or a well-established cellular context for that activity, supported by UniProt and the Falcon literature synthesis. |
| GO:0031625 ubiquitin protein ligase binding | IEA GO_REF:0000120 | KEEP AS NON CORE | Summary: Ubiquitin protein ligase binding is a valid non-core regulatory interaction for p53 stability control. Reason: p53 binding to E3 ligases such as MDM2 and FATS regulates p53 stability, but this is regulatory context rather than the core p53 transcription factor activity. Supporting Evidence: file:mouse/Trp53/Trp53-deep-research-falcon.md MDM2 is a major negative regulator; it ubiquitinates p53 to maintain low basal levels. file:mouse/Trp53/Trp53-uniprot.txt Interacts with C10orf90/FATS; the interaction inhibits binding of TP53 and MDM2. |
| GO:0032211 negative regulation of telomere maintenance via telomerase | IEA GO_REF:0000107 | KEEP AS NON CORE | Summary: negative regulation of telomere maintenance via telomerase is a supported p53-associated context, interaction, localization, or downstream output, but it is not the core Trp53 molecular function. Reason: Retain as non-core because Trp53/p53 is primarily a sequence-specific DNA-binding transcription factor; this term reflects regulatory context, stress response, localization, or a tissue-specific downstream consequence. Supporting Evidence: file:mouse/Trp53/Trp53-deep-research-falcon.md The p53 transcriptional program is tissue-, stress-, and context-dependent; many annotated responses are downstream or specialized outputs rather than the core molecular activity. |
| GO:0032991 protein-containing complex | IEA GO_REF:0000107 | KEEP AS NON CORE | Summary: protein-containing complex is a supported p53-associated context, interaction, localization, or downstream output, but it is not the core Trp53 molecular function. Reason: Retain as non-core because Trp53/p53 is primarily a sequence-specific DNA-binding transcription factor; this term reflects regulatory context, stress response, localization, or a tissue-specific downstream consequence. Supporting Evidence: file:mouse/Trp53/Trp53-deep-research-falcon.md The p53 transcriptional program is tissue-, stress-, and context-dependent; many annotated responses are downstream or specialized outputs rather than the core molecular activity. |
| GO:0033209 tumor necrosis factor-mediated signaling pathway | IEA GO_REF:0000107 | KEEP AS NON CORE | Summary: tumor necrosis factor-mediated signaling pathway is a supported p53-associated context, interaction, localization, or downstream output, but it is not the core Trp53 molecular function. Reason: Retain as non-core because Trp53/p53 is primarily a sequence-specific DNA-binding transcription factor; this term reflects regulatory context, stress response, localization, or a tissue-specific downstream consequence. Supporting Evidence: file:mouse/Trp53/Trp53-deep-research-falcon.md The p53 transcriptional program is tissue-, stress-, and context-dependent; many annotated responses are downstream or specialized outputs rather than the core molecular activity. |
| GO:0034644 cellular response to UV | IEA GO_REF:0000107 | KEEP AS NON CORE | Summary: cellular response to UV is a supported p53-associated context, interaction, localization, or downstream output, but it is not the core Trp53 molecular function. Reason: Retain as non-core because Trp53/p53 is primarily a sequence-specific DNA-binding transcription factor; this term reflects regulatory context, stress response, localization, or a tissue-specific downstream consequence. Supporting Evidence: file:mouse/Trp53/Trp53-deep-research-falcon.md The p53 transcriptional program is tissue-, stress-, and context-dependent; many annotated responses are downstream or specialized outputs rather than the core molecular activity. |
| GO:0036310 ATP-dependent DNA/DNA annealing activity | IEA GO_REF:0000107 | REMOVE | Summary: ATP-dependent DNA/DNA annealing activity is not supported by the reviewed mouse p53 evidence. Reason: The evidence supports sequence-specific DNA binding and transcriptional regulation, not ATP-dependent DNA annealing enzyme activity. |
| GO:0042771 intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator | IEA GO_REF:0000107 | ACCEPT | Summary: intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator is consistent with the curated mouse p53 biology as a stress-activated DNA-binding transcription factor, transcriptional regulator, or well-supported subcellular context. Reason: The term fits the direct p53 activity or a well-established cellular context for that activity, supported by UniProt and the Falcon literature synthesis. |
| GO:0042802 identical protein binding | IEA GO_REF:0000107 | ACCEPT | Summary: identical protein binding is consistent with the curated mouse p53 biology as a stress-activated DNA-binding transcription factor, transcriptional regulator, or well-supported subcellular context. Reason: The term fits the direct p53 activity or a well-established cellular context for that activity, supported by UniProt and the Falcon literature synthesis. |
| GO:0042826 histone deacetylase binding | IEA GO_REF:0000107 | KEEP AS NON CORE | Summary: Histone deacetylase binding is present as an orthology-derived interaction annotation and fits p53 post-translational regulation by deacetylases such as SIRT1, but it is not the core Trp53 molecular function. Reason: Retain as a supported regulatory interaction context rather than treating histone deacetylase binding as a core p53 function. Supporting Evidence: file:mouse/Trp53/Trp53-uniprot.txt Deacetylation of Lys-379 by SIRT1 impairs its ability to induce proapoptotic program and modulate cell senescence. file:mouse/Trp53/Trp53-uniprot.txt GO; GO:0042826; F:histone deacetylase binding; ISO:GO_Central. |
| GO:0043065 positive regulation of apoptotic process | IEA GO_REF:0000120 | ACCEPT | Summary: positive regulation of apoptotic process is consistent with the curated mouse p53 biology as a stress-activated DNA-binding transcription factor, transcriptional regulator, or well-supported subcellular context. Reason: The term fits the direct p53 activity or a well-established cellular context for that activity, supported by UniProt and the Falcon literature synthesis. |
| GO:0043066 negative regulation of apoptotic process | IEA GO_REF:0000107 | MARK AS OVER ANNOTATED | Summary: Negative regulation of apoptotic process is an inferred annotation that does not match the main p53 apoptotic program; p53 is better supported as a positive regulator of intrinsic apoptosis through targets such as Puma/Bbc3, Bax, Noxa, and Apaf1. Reason: The cited support favors p53-induced apoptosis, not a core anti-apoptotic role for Trp53. Supporting Evidence: file:mouse/Trp53/Trp53-deep-research-falcon.md Canonical apoptotic targets include Bbc3/Puma, Bax, Pmaip1/Noxa, and Apaf1. Mouse studies show PUMA is essential for many p53-induced apoptotic responses, while p21 is critical for arrest/senescence. file:mouse/Trp53/Trp53-uniprot.txt GO; GO:0043066; P:negative regulation of apoptotic process; IMP:MGI. |
| GO:0045815 transcription initiation-coupled chromatin remodeling | IEA GO_REF:0000107 | KEEP AS NON CORE | Summary: transcription initiation-coupled chromatin remodeling is a supported p53-associated context, interaction, localization, or downstream output, but it is not the core Trp53 molecular function. Reason: Retain as non-core because Trp53/p53 is primarily a sequence-specific DNA-binding transcription factor; this term reflects regulatory context, stress response, localization, or a tissue-specific downstream consequence. Supporting Evidence: file:mouse/Trp53/Trp53-deep-research-falcon.md The p53 transcriptional program is tissue-, stress-, and context-dependent; many annotated responses are downstream or specialized outputs rather than the core molecular activity. |
| GO:0045892 negative regulation of DNA-templated transcription | IEA GO_REF:0000107 | ACCEPT | Summary: negative regulation of DNA-templated transcription is consistent with the curated mouse p53 biology as a stress-activated DNA-binding transcription factor, transcriptional regulator, or well-supported subcellular context. Reason: The term fits the direct p53 activity or a well-established cellular context for that activity, supported by UniProt and the Falcon literature synthesis. |
| GO:0045893 positive regulation of DNA-templated transcription | IEA GO_REF:0000107 | ACCEPT | Summary: positive regulation of DNA-templated transcription is consistent with the curated mouse p53 biology as a stress-activated DNA-binding transcription factor, transcriptional regulator, or well-supported subcellular context. Reason: The term fits the direct p53 activity or a well-established cellular context for that activity, supported by UniProt and the Falcon literature synthesis. |
| GO:0045899 positive regulation of RNA polymerase II transcription preinitiation complex assembly | IEA GO_REF:0000107 | KEEP AS NON CORE | Summary: positive regulation of RNA polymerase II transcription preinitiation complex assembly is a supported p53-associated context, interaction, localization, or downstream output, but it is not the core Trp53 molecular function. Reason: Retain as non-core because Trp53/p53 is primarily a sequence-specific DNA-binding transcription factor; this term reflects regulatory context, stress response, localization, or a tissue-specific downstream consequence. Supporting Evidence: file:mouse/Trp53/Trp53-deep-research-falcon.md The p53 transcriptional program is tissue-, stress-, and context-dependent; many annotated responses are downstream or specialized outputs rather than the core molecular activity. |
| GO:0046677 response to antibiotic | IEA GO_REF:0000107 | KEEP AS NON CORE | Summary: Response to antibiotic is a broad treatment-response phenotype, not a direct p53 molecular function. Reason: This can be retained as a context-specific stress-response output, but it should not be core for Trp53. Supporting Evidence: file:mouse/Trp53/Trp53-deep-research-falcon.md The p53 transcriptional program is tissue-, stress-, and context-dependent. |
| GO:0046982 protein heterodimerization activity | IEA GO_REF:0000107 | REMOVE | Summary: Protein heterodimerization activity is not supported as a direct p53 activity; p53 forms homodimers and homotetramers. Reason: The reviewed evidence supports p53 homodimerization/homotetrameric DNA binding rather than heterodimerization. Supporting Evidence: file:mouse/Trp53/Trp53-uniprot.txt Forms homodimers and homotetramers. Binds DNA as a homotetramer. |
| GO:0048147 negative regulation of fibroblast proliferation | IEA GO_REF:0000107 | KEEP AS NON CORE | Summary: negative regulation of fibroblast proliferation is supported as a context-specific p53 output or phenotype, but it is not the core molecular function of Trp53. Reason: p53 has broad tissue- and stress-specific downstream effects; this annotation can be retained as non-core rather than treated as the primary evolved activity. Supporting Evidence: file:mouse/Trp53/Trp53-deep-research-falcon.md The p53 transcriptional program is tissue-, stress-, and context-dependent; many annotated responses are downstream or specialized outputs rather than the core molecular activity. |
| GO:0048539 bone marrow development | IEA GO_REF:0000107 | KEEP AS NON CORE | Summary: bone marrow development is supported as a context-specific p53 output or phenotype, but it is not the core molecular function of Trp53. Reason: p53 has broad tissue- and stress-specific downstream effects; this annotation can be retained as non-core rather than treated as the primary evolved activity. Supporting Evidence: file:mouse/Trp53/Trp53-deep-research-falcon.md The p53 transcriptional program is tissue-, stress-, and context-dependent; many annotated responses are downstream or specialized outputs rather than the core molecular activity. |
| GO:0051087 protein-folding chaperone binding | IEA GO_REF:0000107 | KEEP AS NON CORE | Summary: protein-folding chaperone binding is supported as a context-specific p53 output or phenotype, but it is not the core molecular function of Trp53. Reason: p53 has broad tissue- and stress-specific downstream effects; this annotation can be retained as non-core rather than treated as the primary evolved activity. Supporting Evidence: file:mouse/Trp53/Trp53-deep-research-falcon.md The p53 transcriptional program is tissue-, stress-, and context-dependent; many annotated responses are downstream or specialized outputs rather than the core molecular activity. |
| GO:0051721 protein phosphatase 2A binding | IEA GO_REF:0000107 | KEEP AS NON CORE | Summary: protein phosphatase 2A binding is a supported p53-associated context, interaction, localization, or downstream output, but it is not the core Trp53 molecular function. Reason: Retain as non-core because Trp53/p53 is primarily a sequence-specific DNA-binding transcription factor; this term reflects regulatory context, stress response, localization, or a tissue-specific downstream consequence. Supporting Evidence: file:mouse/Trp53/Trp53-deep-research-falcon.md The p53 transcriptional program is tissue-, stress-, and context-dependent; many annotated responses are downstream or specialized outputs rather than the core molecular activity. |
| GO:0051726 regulation of cell cycle | IEA GO_REF:0000107 | ACCEPT | Summary: regulation of cell cycle is consistent with the curated mouse p53 biology as a stress-activated DNA-binding transcription factor, transcriptional regulator, or well-supported subcellular context. Reason: The term fits the direct p53 activity or a well-established cellular context for that activity, supported by UniProt and the Falcon literature synthesis. |
| GO:0060218 hematopoietic stem cell differentiation | IEA GO_REF:0000107 | KEEP AS NON CORE | Summary: hematopoietic stem cell differentiation is supported as a context-specific p53 output or phenotype, but it is not the core molecular function of Trp53. Reason: p53 has broad tissue- and stress-specific downstream effects; this annotation can be retained as non-core rather than treated as the primary evolved activity. Supporting Evidence: file:mouse/Trp53/Trp53-deep-research-falcon.md The p53 transcriptional program is tissue-, stress-, and context-dependent; many annotated responses are downstream or specialized outputs rather than the core molecular activity. |
| GO:0061629 RNA polymerase II-specific DNA-binding transcription factor binding | IEA GO_REF:0000107 | KEEP AS NON CORE | Summary: RNA polymerase II-specific DNA-binding transcription factor binding is a supported p53-associated context, interaction, localization, or downstream output, but it is not the core Trp53 molecular function. Reason: Retain as non-core because Trp53/p53 is primarily a sequence-specific DNA-binding transcription factor; this term reflects regulatory context, stress response, localization, or a tissue-specific downstream consequence. Supporting Evidence: file:mouse/Trp53/Trp53-deep-research-falcon.md The p53 transcriptional program is tissue-, stress-, and context-dependent; many annotated responses are downstream or specialized outputs rather than the core molecular activity. |
| GO:0065003 protein-containing complex assembly | IEA GO_REF:0000107 | KEEP AS NON CORE | Summary: protein-containing complex assembly is a supported p53-associated context, interaction, localization, or downstream output, but it is not the core Trp53 molecular function. Reason: Retain as non-core because Trp53/p53 is primarily a sequence-specific DNA-binding transcription factor; this term reflects regulatory context, stress response, localization, or a tissue-specific downstream consequence. Supporting Evidence: file:mouse/Trp53/Trp53-deep-research-falcon.md The p53 transcriptional program is tissue-, stress-, and context-dependent; many annotated responses are downstream or specialized outputs rather than the core molecular activity. |
| GO:0071456 cellular response to hypoxia | IEA GO_REF:0000120 | KEEP AS NON CORE | Summary: cellular response to hypoxia is a supported p53-associated context, interaction, localization, or downstream output, but it is not the core Trp53 molecular function. Reason: Retain as non-core because Trp53/p53 is primarily a sequence-specific DNA-binding transcription factor; this term reflects regulatory context, stress response, localization, or a tissue-specific downstream consequence. Supporting Evidence: file:mouse/Trp53/Trp53-deep-research-falcon.md The p53 transcriptional program is tissue-, stress-, and context-dependent; many annotated responses are downstream or specialized outputs rather than the core molecular activity. |
| GO:0071466 cellular response to xenobiotic stimulus | IEA GO_REF:0000107 | KEEP AS NON CORE | Summary: cellular response to xenobiotic stimulus is a supported p53-associated context, interaction, localization, or downstream output, but it is not the core Trp53 molecular function. Reason: Retain as non-core because Trp53/p53 is primarily a sequence-specific DNA-binding transcription factor; this term reflects regulatory context, stress response, localization, or a tissue-specific downstream consequence. Supporting Evidence: file:mouse/Trp53/Trp53-deep-research-falcon.md The p53 transcriptional program is tissue-, stress-, and context-dependent; many annotated responses are downstream or specialized outputs rather than the core molecular activity. |
| GO:0071479 cellular response to ionizing radiation | IEA GO_REF:0000107 | KEEP AS NON CORE | Summary: cellular response to ionizing radiation is a supported p53-associated context, interaction, localization, or downstream output, but it is not the core Trp53 molecular function. Reason: Retain as non-core because Trp53/p53 is primarily a sequence-specific DNA-binding transcription factor; this term reflects regulatory context, stress response, localization, or a tissue-specific downstream consequence. Supporting Evidence: file:mouse/Trp53/Trp53-deep-research-falcon.md The p53 transcriptional program is tissue-, stress-, and context-dependent; many annotated responses are downstream or specialized outputs rather than the core molecular activity. |
| GO:0071480 cellular response to gamma radiation | IEA GO_REF:0000107 | KEEP AS NON CORE | Summary: cellular response to gamma radiation is a supported p53-associated context, interaction, localization, or downstream output, but it is not the core Trp53 molecular function. Reason: Retain as non-core because Trp53/p53 is primarily a sequence-specific DNA-binding transcription factor; this term reflects regulatory context, stress response, localization, or a tissue-specific downstream consequence. Supporting Evidence: file:mouse/Trp53/Trp53-deep-research-falcon.md The p53 transcriptional program is tissue-, stress-, and context-dependent; many annotated responses are downstream or specialized outputs rather than the core molecular activity. |
| GO:0072331 signal transduction by p53 class mediator | IEA GO_REF:0000107 | ACCEPT | Summary: signal transduction by p53 class mediator is consistent with the curated mouse p53 biology as a stress-activated DNA-binding transcription factor, transcriptional regulator, or well-supported subcellular context. Reason: The term fits the direct p53 activity or a well-established cellular context for that activity, supported by UniProt and the Falcon literature synthesis. |
| GO:0072332 intrinsic apoptotic signaling pathway by p53 class mediator | IEA GO_REF:0000107 | ACCEPT | Summary: intrinsic apoptotic signaling pathway by p53 class mediator is consistent with the curated mouse p53 biology as a stress-activated DNA-binding transcription factor, transcriptional regulator, or well-supported subcellular context. Reason: The term fits the direct p53 activity or a well-established cellular context for that activity, supported by UniProt and the Falcon literature synthesis. |
| GO:0072717 cellular response to actinomycin D | IEA GO_REF:0000107 | KEEP AS NON CORE | Summary: cellular response to actinomycin D is a supported p53-associated context, interaction, localization, or downstream output, but it is not the core Trp53 molecular function. Reason: Retain as non-core because Trp53/p53 is primarily a sequence-specific DNA-binding transcription factor; this term reflects regulatory context, stress response, localization, or a tissue-specific downstream consequence. Supporting Evidence: file:mouse/Trp53/Trp53-deep-research-falcon.md The p53 transcriptional program is tissue-, stress-, and context-dependent; many annotated responses are downstream or specialized outputs rather than the core molecular activity. |
| GO:0090200 positive regulation of release of cytochrome c from mitochondria | IEA GO_REF:0000107 | KEEP AS NON CORE | Summary: positive regulation of release of cytochrome c from mitochondria is a supported p53-associated context, interaction, localization, or downstream output, but it is not the core Trp53 molecular function. Reason: Retain as non-core because Trp53/p53 is primarily a sequence-specific DNA-binding transcription factor; this term reflects regulatory context, stress response, localization, or a tissue-specific downstream consequence. Supporting Evidence: file:mouse/Trp53/Trp53-deep-research-falcon.md The p53 transcriptional program is tissue-, stress-, and context-dependent; many annotated responses are downstream or specialized outputs rather than the core molecular activity. |
| GO:0090398 cellular senescence | IEA GO_REF:0000107 | ACCEPT | Summary: cellular senescence is consistent with the curated mouse p53 biology as a stress-activated DNA-binding transcription factor, transcriptional regulator, or well-supported subcellular context. Reason: The term fits the direct p53 activity or a well-established cellular context for that activity, supported by UniProt and the Falcon literature synthesis. |
| GO:0090399 replicative senescence | IEA GO_REF:0000107 | ACCEPT | Summary: replicative senescence is consistent with the curated mouse p53 biology as a stress-activated DNA-binding transcription factor, transcriptional regulator, or well-supported subcellular context. Reason: The term fits the direct p53 activity or a well-established cellular context for that activity, supported by UniProt and the Falcon literature synthesis. |
| GO:0090403 oxidative stress-induced premature senescence | IEA GO_REF:0000107 | ACCEPT | Summary: oxidative stress-induced premature senescence is consistent with the curated mouse p53 biology as a stress-activated DNA-binding transcription factor, transcriptional regulator, or well-supported subcellular context. Reason: The term fits the direct p53 activity or a well-established cellular context for that activity, supported by UniProt and the Falcon literature synthesis. |
| GO:0097252 oligodendrocyte apoptotic process | IEA GO_REF:0000107 | KEEP AS NON CORE | Summary: Oligodendrocyte apoptotic process is a tissue-specific p53 apoptotic output, not the core Trp53 molecular function. Reason: Retain as non-core because p53 apoptotic programs are highly tissue- and stress-specific; the core activity is DNA-binding transcriptional regulation of cell-fate targets. Supporting Evidence: file:mouse/Trp53/Trp53-deep-research-falcon.md Tissue specificity / localization of function | p53 functions predominantly in the nucleus as a transcription factor, but outputs are markedly cell- and tissue-specific. |
| GO:0097718 disordered domain specific binding | IEA GO_REF:0000107 | KEEP AS NON CORE | Summary: disordered domain specific binding is supported as a context-specific p53 output or phenotype, but it is not the core molecular function of Trp53. Reason: p53 has broad tissue- and stress-specific downstream effects; this annotation can be retained as non-core rather than treated as the primary evolved activity. Supporting Evidence: file:mouse/Trp53/Trp53-deep-research-falcon.md The p53 transcriptional program is tissue-, stress-, and context-dependent; many annotated responses are downstream or specialized outputs rather than the core molecular activity. |
| GO:0140296 general transcription initiation factor binding | IEA GO_REF:0000107 | KEEP AS NON CORE | Summary: general transcription initiation factor binding is a supported p53-associated context, interaction, localization, or downstream output, but it is not the core Trp53 molecular function. Reason: Retain as non-core because Trp53/p53 is primarily a sequence-specific DNA-binding transcription factor; this term reflects regulatory context, stress response, localization, or a tissue-specific downstream consequence. Supporting Evidence: file:mouse/Trp53/Trp53-deep-research-falcon.md The p53 transcriptional program is tissue-, stress-, and context-dependent; many annotated responses are downstream or specialized outputs rather than the core molecular activity. |
| GO:0140677 molecular function activator activity | IEA GO_REF:0000107 | KEEP AS NON CORE | Summary: molecular function activator activity is a supported p53-associated context, interaction, localization, or downstream output, but it is not the core Trp53 molecular function. Reason: Retain as non-core because Trp53/p53 is primarily a sequence-specific DNA-binding transcription factor; this term reflects regulatory context, stress response, localization, or a tissue-specific downstream consequence. Supporting Evidence: file:mouse/Trp53/Trp53-deep-research-falcon.md The p53 transcriptional program is tissue-, stress-, and context-dependent; many annotated responses are downstream or specialized outputs rather than the core molecular activity. |
| GO:0140693 molecular condensate scaffold activity | IEA GO_REF:0000107 | KEEP AS NON CORE | Summary: Molecular condensate scaffold activity has UniProt support through p53 condensate regulation, but it is specialized regulatory biophysics rather than the core annotation. Reason: Retain as non-core because phase-separation behavior modulates p53 regulation while the main curated function remains DNA-binding transcriptional control. Supporting Evidence: file:mouse/Trp53/Trp53-uniprot.txt Phosphorylation at Ser-389 regulates its ability to undergo liquid-liquid phase separation by increasing fluidity of TP53/p53 condensates |
| GO:1900119 positive regulation of execution phase of apoptosis | IEA GO_REF:0000107 | KEEP AS NON CORE | Summary: positive regulation of execution phase of apoptosis is a supported p53-associated context, interaction, localization, or downstream output, but it is not the core Trp53 molecular function. Reason: Retain as non-core because Trp53/p53 is primarily a sequence-specific DNA-binding transcription factor; this term reflects regulatory context, stress response, localization, or a tissue-specific downstream consequence. Supporting Evidence: file:mouse/Trp53/Trp53-deep-research-falcon.md The p53 transcriptional program is tissue-, stress-, and context-dependent; many annotated responses are downstream or specialized outputs rather than the core molecular activity. |
| GO:1902895 positive regulation of miRNA transcription | IEA GO_REF:0000107 | KEEP AS NON CORE | Summary: positive regulation of miRNA transcription is a supported p53-associated context, interaction, localization, or downstream output, but it is not the core Trp53 molecular function. Reason: Retain as non-core because Trp53/p53 is primarily a sequence-specific DNA-binding transcription factor; this term reflects regulatory context, stress response, localization, or a tissue-specific downstream consequence. Supporting Evidence: file:mouse/Trp53/Trp53-deep-research-falcon.md The p53 transcriptional program is tissue-, stress-, and context-dependent; many annotated responses are downstream or specialized outputs rather than the core molecular activity. |
| GO:1903451 negative regulation of G1 to G0 transition | IEA GO_REF:0000107 | KEEP AS NON CORE | Summary: negative regulation of G1 to G0 transition is a supported p53-associated context, interaction, localization, or downstream output, but it is not the core Trp53 molecular function. Reason: Retain as non-core because Trp53/p53 is primarily a sequence-specific DNA-binding transcription factor; this term reflects regulatory context, stress response, localization, or a tissue-specific downstream consequence. Supporting Evidence: file:mouse/Trp53/Trp53-deep-research-falcon.md The p53 transcriptional program is tissue-, stress-, and context-dependent; many annotated responses are downstream or specialized outputs rather than the core molecular activity. |
| GO:1905856 negative regulation of pentose-phosphate shunt | IEA GO_REF:0000107 | KEEP AS NON CORE | Summary: Negative regulation of the pentose-phosphate shunt is supported by a specific G6PD-containing complex, but it is a specialized metabolic branch of p53 activity. Reason: Retain as non-core because this metabolic regulation is context-specific and downstream of p53 regulatory interactions rather than the central DNA-binding transcription factor function. Supporting Evidence: file:mouse/Trp53/Trp53-uniprot.txt Forms a ternary complex with ALDOB and G6PD; this interaction is direct. ALDOB stabilizes the complex inhibiting G6PD activity and keeping oxidative pentose phosphate metabolism in check. file:mouse/Trp53/Trp53-deep-research-falcon.md Metabolic targets include GLS2 and SCO2 (promote OXPHOS) and repression of GLUT1/GLUT4. |
| GO:1990841 promoter-specific chromatin binding | IEA GO_REF:0000107 | ACCEPT | Summary: promoter-specific chromatin binding is consistent with the curated mouse p53 biology as a stress-activated DNA-binding transcription factor, transcriptional regulator, or well-supported subcellular context. Reason: The term fits the direct p53 activity or a well-established cellular context for that activity, supported by UniProt and the Falcon literature synthesis. |
| GO:2000379 positive regulation of reactive oxygen species metabolic process | IEA GO_REF:0000107 | KEEP AS NON CORE | Summary: positive regulation of reactive oxygen species metabolic process is a supported p53-associated context, interaction, localization, or downstream output, but it is not the core Trp53 molecular function. Reason: Retain as non-core because Trp53/p53 is primarily a sequence-specific DNA-binding transcription factor; this term reflects regulatory context, stress response, localization, or a tissue-specific downstream consequence. Supporting Evidence: file:mouse/Trp53/Trp53-deep-research-falcon.md The p53 transcriptional program is tissue-, stress-, and context-dependent; many annotated responses are downstream or specialized outputs rather than the core molecular activity. |
| GO:2000774 positive regulation of cellular senescence | IEA GO_REF:0000107 | ACCEPT | Summary: positive regulation of cellular senescence is consistent with the curated mouse p53 biology as a stress-activated DNA-binding transcription factor, transcriptional regulator, or well-supported subcellular context. Reason: The term fits the direct p53 activity or a well-established cellular context for that activity, supported by UniProt and the Falcon literature synthesis. |
| GO:2001244 positive regulation of intrinsic apoptotic signaling pathway | IEA GO_REF:0000107 | ACCEPT | Summary: positive regulation of intrinsic apoptotic signaling pathway is consistent with the curated mouse p53 biology as a stress-activated DNA-binding transcription factor, transcriptional regulator, or well-supported subcellular context. Reason: The term fits the direct p53 activity or a well-established cellular context for that activity, supported by UniProt and the Falcon literature synthesis. |
| GO:0140693 molecular condensate scaffold activity | ISS GO_REF:0000024 | KEEP AS NON CORE | Summary: Molecular condensate scaffold activity has UniProt support through p53 condensate regulation, but it is specialized regulatory biophysics rather than the core annotation. Reason: Retain as non-core because phase-separation behavior modulates p53 regulation while the main curated function remains DNA-binding transcriptional control. Supporting Evidence: file:mouse/Trp53/Trp53-uniprot.txt Phosphorylation at Ser-389 regulates its ability to undergo liquid-liquid phase separation by increasing fluidity of TP53/p53 condensates |
| GO:0045944 positive regulation of transcription by RNA polymerase II | IMP PMID:16407291 Endoplasmic reticulum stress-induced apoptosis: multiple pat... | ACCEPT | Summary: p53 positively regulates transcription of PUMA and NOXA during ER stress in MEFs. Reason: PMID:16407291 directly supports p53-dependent transcriptional activation of pro-apoptotic targets in this context. Supporting Evidence: PMID:16407291 ER stress selectively activates BH3-only proteins PUMA and NOXA at the transcript level through the tumor suppressor gene p53. |
| GO:0045944 positive regulation of transcription by RNA polymerase II | ISS PMID:30089260 p53 Regulates the Expression of LRP1 and Apoptosis through a... | ACCEPT | Summary: positive regulation of transcription by RNA polymerase II is consistent with the curated mouse p53 biology as a stress-activated DNA-binding transcription factor, transcriptional regulator, or well-supported subcellular context. Reason: The term fits the direct p53 activity or a well-established cellular context for that activity, supported by UniProt and the Falcon literature synthesis. Supporting Evidence: PMID:30089260 LRP1 transcript expression is upregulated in response to both sub-lethal and lethal doses of p53-activating stress |
| GO:0006357 regulation of transcription by RNA polymerase II | ISS PMID:30089260 p53 Regulates the Expression of LRP1 and Apoptosis through a... | ACCEPT | Summary: regulation of transcription by RNA polymerase II is consistent with the curated mouse p53 biology as a stress-activated DNA-binding transcription factor, transcriptional regulator, or well-supported subcellular context. Reason: The term fits the direct p53 activity or a well-established cellular context for that activity, supported by UniProt and the Falcon literature synthesis. Supporting Evidence: PMID:30089260 Our results define a negative feedback loop involving the p53-regulated coding gene LRP1 and p53-regulated miRNA genes. |
| GO:0006974 DNA damage response | ISS PMID:30089260 p53 Regulates the Expression of LRP1 and Apoptosis through a... | ACCEPT | Summary: DNA damage response is consistent with the curated mouse p53 biology as a stress-activated DNA-binding transcription factor, transcriptional regulator, or well-supported subcellular context. Reason: The term fits the direct p53 activity or a well-established cellular context for that activity, supported by UniProt and the Falcon literature synthesis. Supporting Evidence: PMID:30089260 LRP1 transcript expression is upregulated in response to both sub-lethal and lethal doses of p53-activating stress |
| GO:0005654 nucleoplasm | TAS Reactome:R-MMU-9816449 | ACCEPT | Summary: nucleoplasm is consistent with the curated mouse p53 biology as a stress-activated DNA-binding transcription factor, transcriptional regulator, or well-supported subcellular context. Reason: The term fits the direct p53 activity or a well-established cellular context for that activity, supported by UniProt and the Falcon literature synthesis. |
| GO:2000774 positive regulation of cellular senescence | IMP PMID:10562313 A proinflammatory cytokine inhibits p53 tumor suppressor act... | ACCEPT | Summary: positive regulation of cellular senescence is consistent with the curated mouse p53 biology as a stress-activated DNA-binding transcription factor, transcriptional regulator, or well-supported subcellular context. Reason: The term fits the direct p53 activity or a well-established cellular context for that activity, supported by UniProt and the Falcon literature synthesis. |
| GO:0090398 cellular senescence | ISS GO_REF:0000024 | ACCEPT | Summary: cellular senescence is consistent with the curated mouse p53 biology as a stress-activated DNA-binding transcription factor, transcriptional regulator, or well-supported subcellular context. Reason: The term fits the direct p53 activity or a well-established cellular context for that activity, supported by UniProt and the Falcon literature synthesis. |
| GO:0036310 ATP-dependent DNA/DNA annealing activity | ISS GO_REF:0000024 | REMOVE | Summary: ATP-dependent DNA/DNA annealing activity is not supported by the reviewed mouse p53 evidence. Reason: The evidence supports sequence-specific DNA binding and transcriptional regulation, not ATP-dependent DNA annealing enzyme activity. |
| GO:0000785 chromatin | ISS PMID:30089260 p53 Regulates the Expression of LRP1 and Apoptosis through a... | ACCEPT | Summary: chromatin is consistent with the curated mouse p53 biology as a stress-activated DNA-binding transcription factor, transcriptional regulator, or well-supported subcellular context. Reason: The term fits the direct p53 activity or a well-established cellular context for that activity, supported by UniProt and the Falcon literature synthesis. Supporting Evidence: PMID:30089260 Here, we identify low-density lipoprotein receptor-related protein 1 (LRP1), a transmembrane endocytic receptor, as a p53 target gene. |
| GO:0005813 centrosome | ISS GO_REF:0000024 | KEEP AS NON CORE | Summary: centrosome is supported as a context-specific p53 output or phenotype, but it is not the core molecular function of Trp53. Reason: p53 has broad tissue- and stress-specific downstream effects; this annotation can be retained as non-core rather than treated as the primary evolved activity. Supporting Evidence: file:mouse/Trp53/Trp53-deep-research-falcon.md The p53 transcriptional program is tissue-, stress-, and context-dependent; many annotated responses are downstream or specialized outputs rather than the core molecular activity. |
| GO:0043065 positive regulation of apoptotic process | ISS GO_REF:0000024 | ACCEPT | Summary: positive regulation of apoptotic process is consistent with the curated mouse p53 biology as a stress-activated DNA-binding transcription factor, transcriptional regulator, or well-supported subcellular context. Reason: The term fits the direct p53 activity or a well-established cellular context for that activity, supported by UniProt and the Falcon literature synthesis. |
| GO:0005634 nucleus | IDA PMID:15532030 Coexpression of Brn-3a POU protein with p53 in a population ... | ACCEPT | Summary: nucleus is consistent with the curated mouse p53 biology as a stress-activated DNA-binding transcription factor, transcriptional regulator, or well-supported subcellular context. Reason: The term fits the direct p53 activity or a well-established cellular context for that activity, supported by UniProt and the Falcon literature synthesis. |
| GO:2001244 positive regulation of intrinsic apoptotic signaling pathway | ISS GO_REF:0000024 | ACCEPT | Summary: positive regulation of intrinsic apoptotic signaling pathway is consistent with the curated mouse p53 biology as a stress-activated DNA-binding transcription factor, transcriptional regulator, or well-supported subcellular context. Reason: The term fits the direct p53 activity or a well-established cellular context for that activity, supported by UniProt and the Falcon literature synthesis. |
| GO:0062100 positive regulation of programmed necrotic cell death | IMP PMID:27258785 The long noncoding RNA NRF regulates programmed necrosis and... | KEEP AS NON CORE | Summary: PMID:27258785 supports p53-dependent promotion of programmed necrosis in cardiomyocytes through the NRF-miR-873-RIPK1/RIPK3 axis. Reason: Retain as non-core because this is a cardiomyocyte ischemia/reperfusion output of p53 transcriptional regulation rather than the general core Trp53 function. Supporting Evidence: PMID:27258785 P53 regulates cardiomyocytes necrosis and myocardial I/R injury through NRF and miR-873. file:mouse/Trp53/Trp53-deep-research-falcon.md The p53 transcriptional program is tissue-, stress-, and context-dependent; many annotated responses are downstream or specialized outputs rather than the core molecular activity. |
| GO:0031625 ubiquitin protein ligase binding | IPI PMID:24240685 FATS is an E2-independent ubiquitin ligase that stabilizes p... | KEEP AS NON CORE | Summary: Ubiquitin protein ligase binding is a valid non-core regulatory interaction for p53 stability control. Reason: p53 binding to E3 ligases such as MDM2 and FATS regulates p53 stability, but this is regulatory context rather than the core p53 transcription factor activity. Supporting Evidence: PMID:24240685 FATS acts as a p53 activator by inhibiting Mdm2 binding to p53 file:mouse/Trp53/Trp53-deep-research-falcon.md MDM2 is a major negative regulator; it ubiquitinates p53 to maintain low basal levels. file:mouse/Trp53/Trp53-uniprot.txt Interacts with C10orf90/FATS; the interaction inhibits binding of TP53 and MDM2. |
| GO:0000978 RNA polymerase II cis-regulatory region sequence-specific DNA binding | IDA PMID:24051492 p53 regulates Period2 expression and the circadian clock. | ACCEPT | Summary: PMID:24051492 directly links p53 to Per2 promoter binding, transcriptional repression, and mouse circadian behavior. Reason: PMID:24051492 directly supports p53 binding to the Per2 promoter and repression of BMAL1/CLOCK-mediated Per2 transcription. Supporting Evidence: PMID:24051492 p53 directly binds to the Per2 promoter and represses its transcriptional activation by BMAL1/CLOCK. PMID:24051492 The p53β/β mouse period lengths were 22.8 Β± 0.1 h |
| GO:1990841 promoter-specific chromatin binding | ISS GO_REF:0000024 | ACCEPT | Summary: promoter-specific chromatin binding is consistent with the curated mouse p53 biology as a stress-activated DNA-binding transcription factor, transcriptional regulator, or well-supported subcellular context. Reason: The term fits the direct p53 activity or a well-established cellular context for that activity, supported by UniProt and the Falcon literature synthesis. |
| GO:0042771 intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator | IDA PMID:16213212 Regulation of p53 translation and induction after DNA damage... | ACCEPT | Summary: intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator is consistent with the curated mouse p53 biology as a stress-activated DNA-binding transcription factor, transcriptional regulator, or well-supported subcellular context. Reason: The term fits the direct p53 activity or a well-established cellular context for that activity, supported by UniProt and the Falcon literature synthesis. |
| GO:0071479 cellular response to ionizing radiation | IDA PMID:16213212 Regulation of p53 translation and induction after DNA damage... | KEEP AS NON CORE | Summary: cellular response to ionizing radiation is a supported p53-associated context, interaction, localization, or downstream output, but it is not the core Trp53 molecular function. Reason: Retain as non-core because Trp53/p53 is primarily a sequence-specific DNA-binding transcription factor; this term reflects regulatory context, stress response, localization, or a tissue-specific downstream consequence. Supporting Evidence: file:mouse/Trp53/Trp53-deep-research-falcon.md The p53 transcriptional program is tissue-, stress-, and context-dependent; many annotated responses are downstream or specialized outputs rather than the core molecular activity. |
| GO:0005515 protein binding | IPI PMID:22262176 Ribosomal stress induces L11- and p53-dependent apoptosis in... | REMOVE | Summary: protein binding is not a useful retained annotation for mouse p53 in this review, usually because it is too generic, weakly informative, or not directly tied to the curated p53 activity. Reason: The core Trp53 function is sequence-specific transcriptional regulation of stress-response programs; this term does not add a biologically informative or direct GO assertion. Supporting Evidence: file:mouse/Trp53/Trp53-deep-research-falcon.md Mouse p53 is primarily a sequence-specific DNA-binding transcription factor; generic binding and broad phenotype terms do not capture the curated core activity. |
| GO:0005634 nucleus | IDA PMID:22262176 Ribosomal stress induces L11- and p53-dependent apoptosis in... | ACCEPT | Summary: nucleus is consistent with the curated mouse p53 biology as a stress-activated DNA-binding transcription factor, transcriptional regulator, or well-supported subcellular context. Reason: The term fits the direct p53 activity or a well-established cellular context for that activity, supported by UniProt and the Falcon literature synthesis. |
| GO:0045892 negative regulation of DNA-templated transcription | IMP PMID:23629966 Deacetylation of p53 induces autophagy by suppressing Bmf ex... | ACCEPT | Summary: negative regulation of DNA-templated transcription is consistent with the curated mouse p53 biology as a stress-activated DNA-binding transcription factor, transcriptional regulator, or well-supported subcellular context. Reason: The term fits the direct p53 activity or a well-established cellular context for that activity, supported by UniProt and the Falcon literature synthesis. Supporting Evidence: PMID:23629966 p53 also suppressed Bmf expression in response to other cell death-stimulating agents, including ultraviolet radiation and histone deacetylase inhibitors. |
| GO:0060333 type II interferon-mediated signaling pathway | IMP PMID:23629966 Deacetylation of p53 induces autophagy by suppressing Bmf ex... | KEEP AS NON CORE | Summary: type II interferon-mediated signaling pathway is supported as a context-specific p53 output or phenotype, but it is not the core molecular function of Trp53. Reason: p53 has broad tissue- and stress-specific downstream effects; this annotation can be retained as non-core rather than treated as the primary evolved activity. Supporting Evidence: file:mouse/Trp53/Trp53-deep-research-falcon.md The p53 transcriptional program is tissue-, stress-, and context-dependent; many annotated responses are downstream or specialized outputs rather than the core molecular activity. |
| GO:0005634 nucleus | IDA PMID:16407291 Endoplasmic reticulum stress-induced apoptosis: multiple pat... | ACCEPT | Summary: The PMID:16407291 MEF evidence directly reports nuclear p53 during ER stress. Reason: This supports nuclear localization in the context of the ER-stress apoptotic response. Supporting Evidence: PMID:16407291 In multiple MEF lines, p53 is primarily nuclear and its level is elevated upon ER stress. |
| GO:0070059 intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress | IMP PMID:16407291 Endoplasmic reticulum stress-induced apoptosis: multiple pat... | ACCEPT | Summary: ER stress-induced apoptosis is directly supported in MEFs and is partially p53-dependent through PUMA and NOXA activation. Reason: PMID:16407291 shows p53 contributes to ER stress-induced apoptosis by transcriptionally activating PUMA and NOXA in MEFs. Supporting Evidence: PMID:16407291 In p53(-/-) MEFs, ER stress-induced apoptosis is partially suppressed. |
| GO:0007623 circadian rhythm | IEP PMID:24051492 p53 regulates Period2 expression and the circadian clock. | KEEP AS NON CORE | Summary: PMID:24051492 directly links p53 to Per2 promoter binding, transcriptional repression, and mouse circadian behavior. Reason: The paper supports p53 influence on circadian rhythm behavior, but this is a context-specific organismal output, not core molecular function. Supporting Evidence: PMID:24051492 p53 directly binds to the Per2 promoter and represses its transcriptional activation by BMAL1/CLOCK. PMID:24051492 The p53β/β mouse period lengths were 22.8 Β± 0.1 h |
| GO:0043153 entrainment of circadian clock by photoperiod | IMP PMID:24051492 p53 regulates Period2 expression and the circadian clock. | KEEP AS NON CORE | Summary: PMID:24051492 supports altered light-cue phase-shift behavior in p53-null mice, downstream of p53 repression of Per2. Reason: Retain as non-core because the light-entrainment phenotype is an organismal circadian output, while the direct p53 activity is promoter binding and transcriptional repression. Supporting Evidence: PMID:24051492 phase shift response to a light cue displays an enhanced phase delay response during free running conditions PMID:24051492 p53 directly binds to the Per2 promoter and represses its transcriptional activation by BMAL1/CLOCK. |
| GO:0045892 negative regulation of DNA-templated transcription | IDA PMID:24051492 p53 regulates Period2 expression and the circadian clock. | ACCEPT | Summary: PMID:24051492 directly links p53 to Per2 promoter binding, transcriptional repression, and mouse circadian behavior. Reason: PMID:24051492 directly supports p53 binding to the Per2 promoter and repression of BMAL1/CLOCK-mediated Per2 transcription. Supporting Evidence: PMID:24051492 p53 directly binds to the Per2 promoter and represses its transcriptional activation by BMAL1/CLOCK. PMID:24051492 The p53β/β mouse period lengths were 22.8 Β± 0.1 h |
| GO:0048512 circadian behavior | IMP PMID:24051492 p53 regulates Period2 expression and the circadian clock. | KEEP AS NON CORE | Summary: PMID:24051492 directly links p53 to Per2 promoter binding, transcriptional repression, and mouse circadian behavior. Reason: The paper supports altered circadian behavior in p53-null mice, but this is a context-specific phenotype, not core molecular function. Supporting Evidence: PMID:24051492 p53 directly binds to the Per2 promoter and represses its transcriptional activation by BMAL1/CLOCK. PMID:24051492 The p53β/β mouse period lengths were 22.8 Β± 0.1 h |
| GO:0005739 mitochondrion | IDA PMID:12667443 p53 has a direct apoptogenic role at the mitochondria. | KEEP AS NON CORE | Summary: mitochondrion is a supported p53-associated context, interaction, localization, or downstream output, but it is not the core Trp53 molecular function. Reason: Retain as non-core because Trp53/p53 is primarily a sequence-specific DNA-binding transcription factor; this term reflects regulatory context, stress response, localization, or a tissue-specific downstream consequence. Supporting Evidence: file:mouse/Trp53/Trp53-deep-research-falcon.md The p53 transcriptional program is tissue-, stress-, and context-dependent; many annotated responses are downstream or specialized outputs rather than the core molecular activity. |
| GO:0097252 oligodendrocyte apoptotic process | ISS GO_REF:0000024 | KEEP AS NON CORE | Summary: Oligodendrocyte apoptotic process is a tissue-specific p53 apoptotic output, not the core Trp53 molecular function. Reason: Retain as non-core because p53 apoptotic programs are highly tissue- and stress-specific; the core activity is DNA-binding transcriptional regulation of cell-fate targets. Supporting Evidence: file:mouse/Trp53/Trp53-deep-research-falcon.md Tissue specificity / localization of function | p53 functions predominantly in the nucleus as a transcription factor, but outputs are markedly cell- and tissue-specific. |
| GO:0005515 protein binding | IPI PMID:20599664 A novel role for cardiac ankyrin repeat protein Ankrd1/CARP ... | REMOVE | Summary: protein binding is not a useful retained annotation for mouse p53 in this review, usually because it is too generic, weakly informative, or not directly tied to the curated p53 activity. Reason: The core Trp53 function is sequence-specific transcriptional regulation of stress-response programs; this term does not add a biologically informative or direct GO assertion. Supporting Evidence: file:mouse/Trp53/Trp53-deep-research-falcon.md Mouse p53 is primarily a sequence-specific DNA-binding transcription factor; generic binding and broad phenotype terms do not capture the curated core activity. |
| GO:0008340 determination of adult lifespan | IMP PMID:20818388 Puma is required for p53-induced depletion of adult stem cel... | KEEP AS NON CORE | Summary: determination of adult lifespan is supported as a context-specific p53 output or phenotype, but it is not the core molecular function of Trp53. Reason: p53 has broad tissue- and stress-specific downstream effects; this annotation can be retained as non-core rather than treated as the primary evolved activity. Supporting Evidence: file:mouse/Trp53/Trp53-deep-research-falcon.md The p53 transcriptional program is tissue-, stress-, and context-dependent; many annotated responses are downstream or specialized outputs rather than the core molecular activity. |
| GO:0042771 intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator | IMP PMID:20818388 Puma is required for p53-induced depletion of adult stem cel... | ACCEPT | Summary: intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator is consistent with the curated mouse p53 biology as a stress-activated DNA-binding transcription factor, transcriptional regulator, or well-supported subcellular context. Reason: The term fits the direct p53 activity or a well-established cellular context for that activity, supported by UniProt and the Falcon literature synthesis. |
| GO:0070245 positive regulation of thymocyte apoptotic process | IMP PMID:20818388 Puma is required for p53-induced depletion of adult stem cel... | KEEP AS NON CORE | Summary: Positive regulation of thymocyte apoptotic process is a tissue-specific p53 apoptotic output, not the core Trp53 molecular function. Reason: Retain as non-core because thymocyte apoptosis is a cell-type-specific consequence of p53 activation; the core activity is DNA-binding transcriptional regulation of stress-response targets. Supporting Evidence: file:mouse/Trp53/Trp53-deep-research-falcon.md Radiosensitive tissues such as thymus, bone marrow, spleen, intestinal epithelium preferentially undergo apoptosis PMID:20818388 p53-dependent apoptosis after DNA damage |
| GO:0097371 MDM2/MDM4 family protein binding | IPI PMID:20818388 Puma is required for p53-induced depletion of adult stem cel... | KEEP AS NON CORE | Summary: MDM2/MDM4 family protein binding is a valid p53 regulatory interaction, but it belongs outside the core function set. Reason: Retain as non-core because MDM2/MDM4 binding controls p53 stability and activity, whereas the core Trp53 function is sequence-specific transcriptional regulation of stress-response targets. Supporting Evidence: file:mouse/Trp53/Trp53-uniprot.txt Interacts with MDM2; leading to ubiquitination and proteasomal degradation of TP53. file:mouse/Trp53/Trp53-deep-research-falcon.md MDM4/MDMX restrains p53 transactivation and forms a heterodimer with MDM2 to enhance p53 degradation. |
| GO:0000122 negative regulation of transcription by RNA polymerase II | IMP PMID:19749791 Repression of SHP-1 expression by p53 leads to trkA tyrosine... | ACCEPT | Summary: PMID:19749791 directly supports p53-dependent repression of SHP-1 transcription. Reason: The paper reports endogenous or transfected wild-type p53 repressing SHP-1 expression through the SHP-1 promoter, supporting negative regulation of transcription. Supporting Evidence: PMID:19749791 endogenous wtp53, activated by therapeutic agents, and transfected wtp53 repress expression of SHP-1 PMID:19749791 p53 repression of SHP-1 expression leads to trkA-Y674/Y675 phosphorylation |
| GO:0008285 negative regulation of cell population proliferation | IMP PMID:19749791 Repression of SHP-1 expression by p53 leads to trkA tyrosine... | ACCEPT | Summary: PMID:19749791 supports p53-dependent suppression of breast-cancer cell proliferation through SHP-1 repression and TrkA activation. Reason: The original evidence directly reports suppressed proliferation when wild-type p53 represses SHP-1 and activates TrkA-dependent signaling. Supporting Evidence: PMID:19749791 show suppressed cell proliferation PMID:19749791 p53 repression of SHP-1 expression leads to trkA-Y674/Y675 phosphorylation and trkA-dependent suppression of breast-cancer cell proliferation. |
| GO:0045892 negative regulation of DNA-templated transcription | IMP PMID:19749791 Repression of SHP-1 expression by p53 leads to trkA tyrosine... | ACCEPT | Summary: PMID:19749791 directly supports p53-dependent repression of SHP-1 transcription. Reason: The paper reports endogenous or transfected wild-type p53 repressing SHP-1 expression through the SHP-1 promoter, supporting negative regulation of transcription. Supporting Evidence: PMID:19749791 endogenous wtp53, activated by therapeutic agents, and transfected wtp53 repress expression of SHP-1 PMID:19749791 p53 repression of SHP-1 expression leads to trkA-Y674/Y675 phosphorylation |
| GO:0005515 protein binding | IPI PMID:11672522 Negative control of p53 by Sir2alpha promotes cell survival ... | REMOVE | Summary: protein binding is not a useful retained annotation for mouse p53 in this review, usually because it is too generic, weakly informative, or not directly tied to the curated p53 activity. Reason: The core Trp53 function is sequence-specific transcriptional regulation of stress-response programs; this term does not add a biologically informative or direct GO assertion. Supporting Evidence: file:mouse/Trp53/Trp53-deep-research-falcon.md Mouse p53 is primarily a sequence-specific DNA-binding transcription factor; generic binding and broad phenotype terms do not capture the curated core activity. |
| GO:0003677 DNA binding | ISS GO_REF:0000024 | ACCEPT | Summary: DNA binding is consistent with the curated mouse p53 biology as a stress-activated DNA-binding transcription factor, transcriptional regulator, or well-supported subcellular context. Reason: The term fits the direct p53 activity or a well-established cellular context for that activity, supported by UniProt and the Falcon literature synthesis. |
| GO:0005507 copper ion binding | ISS GO_REF:0000024 | MODIFY | Summary: Copper ion binding is not supported for mouse p53 by the cited evidence; UniProt documents Zn(2+) binding instead. Reason: Replace the unsupported copper-binding term with GO:0008270 zinc ion binding, the metal cofactor documented for p53. Proposed replacements: zinc ion binding Supporting Evidence: file:mouse/Trp53/Trp53-uniprot.txt Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Note=Binds 1 zinc ion per subunit. |
| GO:0005634 nucleus | ISS GO_REF:0000024 | ACCEPT | Summary: nucleus is consistent with the curated mouse p53 biology as a stress-activated DNA-binding transcription factor, transcriptional regulator, or well-supported subcellular context. Reason: The term fits the direct p53 activity or a well-established cellular context for that activity, supported by UniProt and the Falcon literature synthesis. |
| GO:0005730 nucleolus | ISS GO_REF:0000024 | KEEP AS NON CORE | Summary: nucleolus is a supported p53-associated context, interaction, localization, or downstream output, but it is not the core Trp53 molecular function. Reason: Retain as non-core because Trp53/p53 is primarily a sequence-specific DNA-binding transcription factor; this term reflects regulatory context, stress response, localization, or a tissue-specific downstream consequence. Supporting Evidence: file:mouse/Trp53/Trp53-deep-research-falcon.md The p53 transcriptional program is tissue-, stress-, and context-dependent; many annotated responses are downstream or specialized outputs rather than the core molecular activity. |
| GO:0005737 cytoplasm | ISS GO_REF:0000024 | KEEP AS NON CORE | Summary: cytoplasm is a supported p53-associated context, interaction, localization, or downstream output, but it is not the core Trp53 molecular function. Reason: Retain as non-core because Trp53/p53 is primarily a sequence-specific DNA-binding transcription factor; this term reflects regulatory context, stress response, localization, or a tissue-specific downstream consequence. Supporting Evidence: file:mouse/Trp53/Trp53-deep-research-falcon.md The p53 transcriptional program is tissue-, stress-, and context-dependent; many annotated responses are downstream or specialized outputs rather than the core molecular activity. |
| GO:0005739 mitochondrion | ISS GO_REF:0000024 | KEEP AS NON CORE | Summary: mitochondrion is a supported p53-associated context, interaction, localization, or downstream output, but it is not the core Trp53 molecular function. Reason: Retain as non-core because Trp53/p53 is primarily a sequence-specific DNA-binding transcription factor; this term reflects regulatory context, stress response, localization, or a tissue-specific downstream consequence. Supporting Evidence: file:mouse/Trp53/Trp53-deep-research-falcon.md The p53 transcriptional program is tissue-, stress-, and context-dependent; many annotated responses are downstream or specialized outputs rather than the core molecular activity. |
| GO:0006289 nucleotide-excision repair | ISS GO_REF:0000024 | ACCEPT | Summary: nucleotide-excision repair is consistent with the curated mouse p53 biology as a stress-activated DNA-binding transcription factor, transcriptional regulator, or well-supported subcellular context. Reason: The term fits the direct p53 activity or a well-established cellular context for that activity, supported by UniProt and the Falcon literature synthesis. |
| GO:0030308 negative regulation of cell growth | ISS GO_REF:0000024 | ACCEPT | Summary: negative regulation of cell growth is consistent with the curated mouse p53 biology as a stress-activated DNA-binding transcription factor, transcriptional regulator, or well-supported subcellular context. Reason: The term fits the direct p53 activity or a well-established cellular context for that activity, supported by UniProt and the Falcon literature synthesis. |
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Download this section (compressed HTML)Q: Which mouse tissues use conserved p53 target programs versus tissue-specific target genes after comparable stress inputs?
Q: Which mitochondrial and cytoplasmic p53 annotations should be retained as direct activities rather than stress-context localizations?
Experiment: Use Trp53 response-reporter mouse cells with matched DNA damage, hypoxia, and metabolic stress treatments to map direct p53 binding, target transcription, and cell fate outputs by tissue.
Experiment: Compare wild-type and DNA-binding-defective Trp53 alleles in primary mouse cells to separate direct transcriptional effects from non-transcriptional mitochondrial apoptosis functions.
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