Chrna9 encodes the alpha9 subunit of an acetylcholine-gated cation channel associated with efferent signaling to inner-ear hair cells. Rat alpha9 can form functional homomers in oocytes, but the native auditory receptor includes alpha10. Calcium entry through the receptor recruits calcium-dependent potassium channels, allowing a cation channel to mediate net inhibition of hair cells. Rat alpha9/alpha10 receptors have high relative calcium permeability compared with chicken; choline is a partial agonist in rat. Nicotine antagonizes the ordinary alpha9/alpha10 channel rather than serving as its canonical activating agonist.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0004888 transmembrane signaling receptor activity | IEA GO_REF:0000002 | MODIFY | Summary: Rat alpha9/alpha10 receptor assays resolve the channel to acetylcholine-gated cation transport. Reason: The subunit supplies part of the pentameric pore and gating machinery; it is not being treated as a complete isolated channel. Refine to the existing specific MF. The original IEA assertion (GO_REF:0000002) is retained in the payload; this assessment uses independent receptor measurements rather than a GO annotation as evidence. Proposed replacements: acetylcholine-gated monoatomic cation-selective channel activity |
| GO:0005216 monoatomic ion channel activity | IEA GO_REF:0000002 | MODIFY | Summary: Rat alpha9/alpha10 receptor assays resolve the channel to acetylcholine-gated cation transport. Reason: The subunit supplies part of the pentameric pore and gating machinery; it is not being treated as a complete isolated channel. Refine to the existing specific MF. The original IEA assertion (GO_REF:0000002) is retained in the payload; this assessment uses independent receptor measurements rather than a GO annotation as evidence. Proposed replacements: acetylcholine-gated monoatomic cation-selective channel activity |
| GO:0005230 extracellular ligand-gated monoatomic ion channel activity | IEA GO_REF:0000002 | MODIFY | Summary: Rat alpha9/alpha10 receptor assays resolve the channel to acetylcholine-gated cation transport. Reason: The subunit supplies part of the pentameric pore and gating machinery; it is not being treated as a complete isolated channel. Refine to the existing specific MF. The original IEA assertion (GO_REF:0000002) is retained in the payload; this assessment uses independent receptor measurements rather than a GO annotation as evidence. Proposed replacements: acetylcholine-gated monoatomic cation-selective channel activity |
| GO:0005886 plasma membrane | EXP PMID:10713500 High calcium permeability and calcium block of the alpha9 ni... | ACCEPT | Summary: Recombinant currents demonstrate functional surface channels; native hair-cell experiments establish the physiological membrane pool. Reason: Rat recombinant/native comparisons are used at their stated species and assay scope. The original EXP assertion (PMID:10713500) is retained in the payload; this assessment uses independent receptor measurements rather than a GO annotation as evidence. |
| GO:0005886 plasma membrane | EXP PMID:11248107 alpha10: a determinant of nicotinic cholinergic receptor fun... | ACCEPT | Summary: Recombinant currents demonstrate functional surface channels; native hair-cell experiments establish the physiological membrane pool. Reason: Rat recombinant/native comparisons are used at their stated species and assay scope. The original EXP assertion (PMID:11248107) is retained in the payload; this assessment uses independent receptor measurements rather than a GO annotation as evidence. |
| GO:0005886 plasma membrane | EXP PMID:12117536 The alpha9alpha10 nicotinic acetylcholine receptor is permea... | ACCEPT | Summary: Recombinant currents demonstrate functional surface channels; native hair-cell experiments establish the physiological membrane pool. Reason: Rat recombinant/native comparisons are used at their stated species and assay scope. The original EXP assertion (PMID:12117536) is retained in the payload; this assessment uses independent receptor measurements rather than a GO annotation as evidence. |
| GO:0005886 plasma membrane | EXP PMID:7954834 Alpha 9: an acetylcholine receptor with novel pharmacologica... | ACCEPT | Summary: Recombinant currents demonstrate functional surface channels; native hair-cell experiments establish the physiological membrane pool. Reason: Rat recombinant/native comparisons are used at their stated species and assay scope. The original EXP assertion (PMID:7954834) is retained in the payload; this assessment uses independent receptor measurements rather than a GO annotation as evidence. |
| GO:0005886 plasma membrane | IBA GO_REF:0000033 | ACCEPT | Summary: Recombinant currents demonstrate functional surface channels; native hair-cell experiments establish the physiological membrane pool. Reason: Rat recombinant/native comparisons are used at their stated species and assay scope. The original IBA assertion (GO_REF:0000033) is retained in the payload; this assessment uses independent receptor measurements rather than a GO annotation as evidence. |
| GO:0005886 plasma membrane | IEA GO_REF:0000120 | ACCEPT | Summary: Recombinant currents demonstrate functional surface channels; native hair-cell experiments establish the physiological membrane pool. Reason: Rat recombinant/native comparisons are used at their stated species and assay scope. The original IEA assertion (GO_REF:0000120) is retained in the payload; this assessment uses independent receptor measurements rather than a GO annotation as evidence. |
| GO:0005886 plasma membrane | ISO GO_REF:0000121 | ACCEPT | Summary: Recombinant currents demonstrate functional surface channels; native hair-cell experiments establish the physiological membrane pool. Reason: Rat recombinant/native comparisons are used at their stated species and assay scope. The original ISO assertion (GO_REF:0000121) is retained in the payload; this assessment uses independent receptor measurements rather than a GO annotation as evidence. |
| GO:0005886 plasma membrane | TAS Reactome:R-RNO-9667850 | ACCEPT | Summary: Recombinant currents demonstrate functional surface channels; native hair-cell experiments establish the physiological membrane pool. Reason: Rat recombinant/native comparisons are used at their stated species and assay scope. The original TAS assertion (Reactome:R-RNO-9667850) is retained in the payload; this assessment uses independent receptor measurements rather than a GO annotation as evidence. |
| GO:0005886 plasma membrane | TAS Reactome:R-RNO-9667857 | ACCEPT | Summary: Recombinant currents demonstrate functional surface channels; native hair-cell experiments establish the physiological membrane pool. Reason: Rat recombinant/native comparisons are used at their stated species and assay scope. The original TAS assertion (Reactome:R-RNO-9667857) is retained in the payload; this assessment uses independent receptor measurements rather than a GO annotation as evidence. |
| GO:0005892 acetylcholine-gated channel complex | IDA PMID:12401316 Coexpression of alpha 9 and alpha 10 nicotinic acetylcholine... | ACCEPT | Summary: Coexpressed alpha9 and alpha10 form functional acetylcholine-gated receptor complexes. Reason: Rat recombinant/native comparisons are used at their stated species and assay scope. The original IDA assertion (PMID:12401316) is retained in the payload; this assessment uses independent receptor measurements rather than a GO annotation as evidence. |
| GO:0005892 acetylcholine-gated channel complex | IEA GO_REF:0000120 | ACCEPT | Summary: Coexpressed alpha9 and alpha10 form functional acetylcholine-gated receptor complexes. Reason: Rat recombinant/native comparisons are used at their stated species and assay scope. The original IEA assertion (GO_REF:0000120) is retained in the payload; this assessment uses independent receptor measurements rather than a GO annotation as evidence. |
| GO:0005892 acetylcholine-gated channel complex | ISO GO_REF:0000121 | ACCEPT | Summary: Coexpressed alpha9 and alpha10 form functional acetylcholine-gated receptor complexes. Reason: Rat recombinant/native comparisons are used at their stated species and assay scope. The original ISO assertion (GO_REF:0000121) is retained in the payload; this assessment uses independent receptor measurements rather than a GO annotation as evidence. |
| GO:0006811 monoatomic ion transport | IEA GO_REF:0000002 | MODIFY | Summary: The measured receptor conducts cations across the membrane, including calcium. Reason: Refine the broad transport term to cation transmembrane transport, preserving monovalent and divalent flux rather than narrowing the entire current to calcium. The original IEA assertion (GO_REF:0000002) is retained in the payload; this assessment uses independent receptor measurements rather than a GO annotation as evidence. Proposed replacements: monoatomic cation transmembrane transport |
| GO:0006812 monoatomic cation transport | ISO GO_REF:0000121 | MODIFY | Summary: The measured receptor conducts cations across the membrane, including calcium. Reason: Refine the broad transport term to cation transmembrane transport, preserving monovalent and divalent flux rather than narrowing the entire current to calcium. The original ISO assertion (GO_REF:0000121) is retained in the payload; this assessment uses independent receptor measurements rather than a GO annotation as evidence. Propagation Review Root cause: NO FAILURE CORE Sources checked: MGI:MGI:1202403 SUPPORTS TRANSFER The measured receptor conducts cations across the membrane, including calcium. Refine the broad transport term to cation transmembrane transport, preserving monovalent and divalent flux rather than narrowing the entire current to calcium. The original ISO assertion (GO_REF:0000121) is retained in the payload; this assessment uses independent receptor measurements rather than a GO annotation as evidence. Proposed replacements: monoatomic cation transmembrane transport |
| GO:0006816 calcium ion transport | IBA GO_REF:0000033 | ACCEPT | Summary: Alpha9/alpha10 channels conduct calcium; direct rat measurements and the human functional study support the conserved subunit contribution. Reason: This term states calcium transport, not calcium exclusivity or identical permeability across species. |
| GO:0007204 positive regulation of cytosolic calcium ion concentration | ISO GO_REF:0000121 | ACCEPT | Summary: Receptor-mediated calcium entry increases cytosolic calcium and recruits downstream conductances; it is not simply calcium release from stores. Reason: Rat recombinant/native comparisons are used at their stated species and assay scope. The original ISO assertion (GO_REF:0000121) is retained in the payload; this assessment uses independent receptor measurements rather than a GO annotation as evidence. |
| GO:0007271 synaptic transmission, cholinergic | IBA GO_REF:0000033 | ACCEPT | Summary: The receptor performs the postsynaptic receiver step of efferent cholinergic transmission. Reason: Rat recombinant/native comparisons are used at their stated species and assay scope. The original IBA assertion (GO_REF:0000033) is retained in the payload; this assessment uses independent receptor measurements rather than a GO annotation as evidence. |
| GO:0010996 response to auditory stimulus | IEA GO_REF:0000107 | KEEP AS NON CORE | Summary: Native auditory experiments and alpha9/alpha10 knockout studies establish efferent modulation of cochlear responses. Reason: Retain a role in auditory response at that resolution, without calling the receptor a mechanically gated sound sensor or inferring all mammalian perceptual phenotypes in this species. |
| GO:0010996 response to auditory stimulus | ISO GO_REF:0000121 | KEEP AS NON CORE | Summary: Native auditory experiments and alpha9/alpha10 knockout studies establish efferent modulation of cochlear responses. Reason: Retain a role in auditory response at that resolution, without calling the receptor a mechanically gated sound sensor or inferring all mammalian perceptual phenotypes in this species. |
| GO:0016020 membrane | IEA GO_REF:0000002 | MODIFY | Summary: The relevant auditory receptor pool is on the postsynaptic hair-cell plasma membrane. Reason: Refine this general membrane location to the already represented postsynaptic membrane; recombinant surface currents corroborate membrane insertion but are not native localization measurements. The original IEA assertion (GO_REF:0000002) is retained in the payload; this assessment uses independent receptor measurements rather than a GO annotation as evidence. Proposed replacements: postsynaptic membrane |
| GO:0022848 acetylcholine-gated monoatomic cation-selective channel activity | IDA PMID:7954834 Alpha 9: an acetylcholine receptor with novel pharmacologica... | ACCEPT | Summary: ACh evokes cation currents through the receptor assembled from these subunits. Reason: Rat recombinant/native comparisons are used at their stated species and assay scope. The original IDA assertion (PMID:7954834) is retained in the payload; this assessment uses independent receptor measurements rather than a GO annotation as evidence. |
| GO:0022848 acetylcholine-gated monoatomic cation-selective channel activity | IEA GO_REF:0000120 | ACCEPT | Summary: ACh evokes cation currents through the receptor assembled from these subunits. Reason: Rat recombinant/native comparisons are used at their stated species and assay scope. The original IEA assertion (GO_REF:0000120) is retained in the payload; this assessment uses independent receptor measurements rather than a GO annotation as evidence. |
| GO:0022848 acetylcholine-gated monoatomic cation-selective channel activity | ISO GO_REF:0000121 | ACCEPT | Summary: ACh evokes cation currents through the receptor assembled from these subunits. Reason: Rat recombinant/native comparisons are used at their stated species and assay scope. The original ISO assertion (GO_REF:0000121) is retained in the payload; this assessment uses independent receptor measurements rather than a GO annotation as evidence. |
| GO:0022848 acetylcholine-gated monoatomic cation-selective channel activity | ISO GO_REF:0000121 | ACCEPT | Summary: ACh evokes cation currents through the receptor assembled from these subunits. Reason: Rat recombinant/native comparisons are used at their stated species and assay scope. The original ISO assertion (GO_REF:0000121) is retained in the payload; this assessment uses independent receptor measurements rather than a GO annotation as evidence. |
| GO:0022848 acetylcholine-gated monoatomic cation-selective channel activity | ISO GO_REF:0000121 | ACCEPT | Summary: ACh evokes cation currents through the receptor assembled from these subunits. Reason: Rat recombinant/native comparisons are used at their stated species and assay scope. The original ISO assertion (GO_REF:0000121) is retained in the payload; this assessment uses independent receptor measurements rather than a GO annotation as evidence. |
| GO:0034220 monoatomic ion transmembrane transport | IBA GO_REF:0000033 | MODIFY | Summary: The measured receptor conducts cations across the membrane, including calcium. Reason: Refine the broad transport term to cation transmembrane transport, preserving monovalent and divalent flux rather than narrowing the entire current to calcium. The original IBA assertion (GO_REF:0000033) is retained in the payload; this assessment uses independent receptor measurements rather than a GO annotation as evidence. Propagation Review Root cause: NO FAILURE CORE Sources checked: PANTHER:PTN000434994 SUPPORTS TRANSFER The inherited broad claim is retained as biologically sound; independent target receptor evidence supports the stated refinement. The historical IBD placement was not re-inferred. Proposed replacements: monoatomic cation transmembrane transport |
| GO:0034220 monoatomic ion transmembrane transport | IEA GO_REF:0000002 | MODIFY | Summary: The measured receptor conducts cations across the membrane, including calcium. Reason: Refine the broad transport term to cation transmembrane transport, preserving monovalent and divalent flux rather than narrowing the entire current to calcium. The original IEA assertion (GO_REF:0000002) is retained in the payload; this assessment uses independent receptor measurements rather than a GO annotation as evidence. Proposed replacements: monoatomic cation transmembrane transport |
| GO:0042472 inner ear morphogenesis | ISO GO_REF:0000121 | KEEP AS NON CORE | Summary: The mouse alpha9 knockout source PMID:10402196 establishes abnormal maturation of cochlear efferent synaptic connections. Reason: Retain this as an inferred, anatomically restricted contribution to inner-ear organization in rat, not a claim that alpha9 constructs the whole membranous labyrinth. The receptor-mediated activity is the plausible developmental contribution; direct rat morphogenetic experiments were not established here. |
| GO:0045211 postsynaptic membrane | IEA GO_REF:0000002 | ACCEPT | Summary: Native auditory receptors occupy the hair-cell postsynaptic membrane facing efferent cholinergic terminals. Reason: Rat recombinant/native comparisons are used at their stated species and assay scope. The original IEA assertion (GO_REF:0000002) is retained in the payload; this assessment uses independent receptor measurements rather than a GO annotation as evidence. |
| GO:0050910 detection of mechanical stimulus involved in sensory perception of sound | ISO GO_REF:0000121 | MARK AS OVER ANNOTATED | Summary: The live GO definition specifies receipt of sound vibration and conversion into a molecular signal. Reason: Alpha9/alpha10 instead receives efferent acetylcholine and modulates the sensory cell through ion-channel signaling. Native comparison and mouse knockout experiments support efferent control and synaptic integrity, not assignment of this receptor as the mechanical stimulus detector. This is a role-scope correction, not a claim of absent auditory function or an invented loss in the PAINT lineage. Propagation Review Root cause: TERM SCOPING PROBLEM Sources checked: MGI:MGI:1202403 UNRESOLVED The live GO definition specifies receipt of sound vibration and conversion into a molecular signal. Alpha9/alpha10 instead receives efferent acetylcholine and modulates the sensory cell through ion-channel signaling. Native comparison and mouse knockout experiments support efferent control and synaptic integrity, not assignment of this receptor as the mechanical stimulus detector. This is a role-scope correction, not a claim of absent auditory function or an invented loss in the PAINT lineage. |
| GO:0051899 membrane depolarization | IBA GO_REF:0000033 | ACCEPT | Summary: The receptor itself produces an inward, depolarizing cation current under the tested ionic conditions. Reason: In native hair cells this calcium entry recruits a separate potassium conductance that can dominate the net response and hyperpolarize the cell. Retain the direct depolarizing component without equating it with net excitatory auditory transmission. |
| GO:0051899 membrane depolarization | IEA GO_REF:0000107 | ACCEPT | Summary: The receptor itself produces an inward, depolarizing cation current under the tested ionic conditions. Reason: In native hair cells this calcium entry recruits a separate potassium conductance that can dominate the net response and hyperpolarize the cell. Retain the direct depolarizing component without equating it with net excitatory auditory transmission. |
| GO:0051899 membrane depolarization | ISO GO_REF:0000121 | ACCEPT | Summary: The receptor itself produces an inward, depolarizing cation current under the tested ionic conditions. Reason: In native hair cells this calcium entry recruits a separate potassium conductance that can dominate the net response and hyperpolarize the cell. Retain the direct depolarizing component without equating it with net excitatory auditory transmission. |
| GO:0051899 membrane depolarization | ISO GO_REF:0000121 | ACCEPT | Summary: The receptor itself produces an inward, depolarizing cation current under the tested ionic conditions. Reason: In native hair cells this calcium entry recruits a separate potassium conductance that can dominate the net response and hyperpolarize the cell. Retain the direct depolarizing component without equating it with net excitatory auditory transmission. |
| GO:0060078 regulation of postsynaptic membrane potential | IEA GO_REF:0000120 | ACCEPT | Summary: Cation entry and downstream potassium-channel coupling regulate the hair-cell postsynaptic potential; the net sign can be inhibitory. Reason: Rat recombinant/native comparisons are used at their stated species and assay scope. The original IEA assertion (GO_REF:0000120) is retained in the payload; this assessment uses independent receptor measurements rather than a GO annotation as evidence. |
| GO:0060079 excitatory postsynaptic potential | IEA GO_REF:0000108 | MARK AS OVER ANNOTATED | Summary: The logical inference from acetylcholine-gated cation-channel activity to an excitatory postsynaptic potential overstates the physiological sign. Reason: Native alpha9-containing hair-cell receptors couple calcium entry to calcium-dependent potassium channels and inhibitory synaptic responses. Real inward cation current is retained separately; it does not by itself establish a net EPSP in the native sensory circuit. Supporting Evidence: PMID:10875922 neonatal inner hair cells are inhibited by cholinergic synaptic input before the onset of hearing. |
| GO:0070373 negative regulation of ERK1 and ERK2 cascade | IEA GO_REF:0000117 | KEEP AS NON CORE | Summary: Full PMID:20505147 tests rat RBL-2H3 mast/basophil cells: prolonged low-dose nicotine inhibits Fc-epsilon-RI-associated ERK signaling and cytokine/leukotriene production, with receptor-subunit knockdowns affecting the suppressive response. Reason: Retain this context-specific signaling contribution. The proposed alpha7/alpha9/alpha10 hybrid is not a structurally established pentamer and the experiment does not establish nicotine agonism of the ordinary hair-cell alpha9alpha10 channel. Supporting Evidence: |
| GO:0070373 negative regulation of ERK1 and ERK2 cascade | IMP PMID:20505147 Nicotine inhibits Fc epsilon RI-induced cysteinyl leukotrien... | KEEP AS NON CORE | Summary: Full PMID:20505147 tests rat RBL-2H3 mast/basophil cells: prolonged low-dose nicotine inhibits Fc-epsilon-RI-associated ERK signaling and cytokine/leukotriene production, with receptor-subunit knockdowns affecting the suppressive response. Reason: Retain this context-specific signaling contribution. The proposed alpha7/alpha9/alpha10 hybrid is not a structurally established pentamer and the experiment does not establish nicotine agonism of the ordinary hair-cell alpha9alpha10 channel. Supporting Evidence: |
| GO:0080164 regulation of nitric oxide metabolic process | ISO GO_REF:0000121 | UNDECIDED | Summary: The transfer names human CHRNA9/Q9UGM1 (including a processed-chain identifier), but the current human review and inspected human channel/structural sources do not identify the nitric-oxide pathway experiment underlying this rat claim. Reason: Neither calcium permeability nor expression establishes that specific metabolic regulation. The source mechanism remains unresolved; this is not evidence that nitric-oxide effects are impossible. Propagation Review Root cause: UNRESOLVED Sources checked: UniProtKB:Q9UGM1 UNRESOLVED The transfer names human CHRNA9/Q9UGM1 (including a processed-chain identifier), but the current human review and inspected human channel/structural sources do not identify the nitric-oxide pathway experiment underlying this rat claim. Neither calcium permeability nor expression establishes that specific metabolic regulation. The source mechanism remains unresolved; this is not evidence that nitric-oxide effects are impossible. UniProtKB:Q9UGM1-PRO_0000000371 UNRESOLVED The transfer names human CHRNA9/Q9UGM1 (including a processed-chain identifier), but the current human review and inspected human channel/structural sources do not identify the nitric-oxide pathway experiment underlying this rat claim. Neither calcium permeability nor expression establishes that specific metabolic regulation. The source mechanism remains unresolved; this is not evidence that nitric-oxide effects are impossible. |
| GO:0095500 acetylcholine receptor signaling pathway | IEA GO_REF:0000107 | ACCEPT | Summary: ACh binding is coupled to channel gating and calcium-dependent downstream signaling. Reason: Rat recombinant/native comparisons are used at their stated species and assay scope. The original IEA assertion (GO_REF:0000107) is retained in the payload; this assessment uses independent receptor measurements rather than a GO annotation as evidence. |
| GO:0095500 acetylcholine receptor signaling pathway | ISO GO_REF:0000121 | ACCEPT | Summary: ACh binding is coupled to channel gating and calcium-dependent downstream signaling. Reason: Rat recombinant/native comparisons are used at their stated species and assay scope. The original ISO assertion (GO_REF:0000121) is retained in the payload; this assessment uses independent receptor measurements rather than a GO annotation as evidence. |
| GO:0097060 synaptic membrane | IEA GO_REF:0000044 | MODIFY | Summary: The relevant auditory receptor pool is on the postsynaptic hair-cell plasma membrane. Reason: Refine this general membrane location to the already represented postsynaptic membrane; recombinant surface currents corroborate membrane insertion but are not native localization measurements. The original IEA assertion (GO_REF:0000044) is retained in the payload; this assessment uses independent receptor measurements rather than a GO annotation as evidence. Proposed replacements: postsynaptic membrane |
| GO:0098981 cholinergic synapse | EXP PMID:10875922 Cholinergic synaptic inhibition of inner hair cells in the n... | ACCEPT | Summary: Native hair-cell cholinergic synapses contain the alpha9/alpha10 receptor pair. Reason: Rat recombinant/native comparisons are used at their stated species and assay scope. The original EXP assertion (PMID:10875922) is retained in the payload; this assessment uses independent receptor measurements rather than a GO annotation as evidence. |
| GO:0098981 cholinergic synapse | IDA PMID:10875922 Cholinergic synaptic inhibition of inner hair cells in the n... | ACCEPT | Summary: Native hair-cell cholinergic synapses contain the alpha9/alpha10 receptor pair. Reason: Rat recombinant/native comparisons are used at their stated species and assay scope. The original IDA assertion (PMID:10875922) is retained in the payload; this assessment uses independent receptor measurements rather than a GO annotation as evidence. |
| GO:0098981 cholinergic synapse | IDA PMID:18420419 Muscle-like nicotinic receptor accessory molecules in sensor... | ACCEPT | Summary: Native hair-cell cholinergic synapses contain the alpha9/alpha10 receptor pair. Reason: Rat recombinant/native comparisons are used at their stated species and assay scope. The original IDA assertion (PMID:18420419) is retained in the payload; this assessment uses independent receptor measurements rather than a GO annotation as evidence. |
| GO:0098981 cholinergic synapse | IEA GO_REF:0000117 | ACCEPT | Summary: Native hair-cell cholinergic synapses contain the alpha9/alpha10 receptor pair. Reason: Rat recombinant/native comparisons are used at their stated species and assay scope. The original IEA assertion (GO_REF:0000117) is retained in the payload; this assessment uses independent receptor measurements rather than a GO annotation as evidence. |
| GO:0099565 chemical synaptic transmission, postsynaptic | IEA GO_REF:0000108 | ACCEPT | Summary: This receptor mediates postsynaptic reception of efferent transmitter; it is not the presynaptic ACh release machinery. Reason: Rat recombinant/native comparisons are used at their stated species and assay scope. The original IEA assertion (GO_REF:0000108) is retained in the payload; this assessment uses independent receptor measurements rather than a GO annotation as evidence. |
| GO:0099634 postsynaptic specialization membrane | IDA PMID:18420419 Muscle-like nicotinic receptor accessory molecules in sensor... | ACCEPT | Summary: The native receptor pool is in the specialized hair-cell postsynaptic membrane. Reason: Rat recombinant/native comparisons are used at their stated species and assay scope. The original IDA assertion (PMID:18420419) is retained in the payload; this assessment uses independent receptor measurements rather than a GO annotation as evidence. |
| GO:1902495 transmembrane transporter complex | IBA GO_REF:0000033 | MODIFY | Summary: The complex is specifically an acetylcholine-gated receptor pentamer. Reason: Refine the inherited general transmembrane-transporter-complex annotation to that identity; no exact native stoichiometry is deduced from a general family descriptor. The original IBA assertion (GO_REF:0000033) is retained in the payload; this assessment uses independent receptor measurements rather than a GO annotation as evidence. Propagation Review Root cause: NO FAILURE CORE Sources checked: PANTHER:PTN000434994 SUPPORTS TRANSFER The inherited broad claim is retained as biologically sound; independent target receptor evidence supports the stated refinement. The historical IBD placement was not re-inferred. Proposed replacements: acetylcholine-gated channel complex |
| GO:1904315 transmitter-gated monoatomic ion channel activity involved in regulation of postsynaptic membrane potential | EXP PMID:10875922 Cholinergic synaptic inhibition of inner hair cells in the n... | MODIFY | Summary: Rat alpha9/alpha10 receptor assays resolve the channel to acetylcholine-gated cation transport. Reason: The subunit supplies part of the pentameric pore and gating machinery; it is not being treated as a complete isolated channel. Refine to the existing specific MF. The original EXP assertion (PMID:10875922) is retained in the payload; this assessment uses independent receptor measurements rather than a GO annotation as evidence. Proposed replacements: acetylcholine-gated monoatomic cation-selective channel activity |
| GO:1904315 transmitter-gated monoatomic ion channel activity involved in regulation of postsynaptic membrane potential | IDA PMID:10875922 Cholinergic synaptic inhibition of inner hair cells in the n... | MODIFY | Summary: Rat alpha9/alpha10 receptor assays resolve the channel to acetylcholine-gated cation transport. Reason: The subunit supplies part of the pentameric pore and gating machinery; it is not being treated as a complete isolated channel. Refine to the existing specific MF. The original IDA assertion (PMID:10875922) is retained in the payload; this assessment uses independent receptor measurements rather than a GO annotation as evidence. Proposed replacements: acetylcholine-gated monoatomic cation-selective channel activity |
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