Caenorhabditis elegans ABI-1 is the sole nematode member of the Abl-interactor adaptor family (PANTHER subfamily PTHR10460:SF0) and an essential subunit of the heteropentameric WAVE/SCAR regulatory complex alongside WVE-1, GEX-2, GEX-3, and BRK-1. Through its N-terminal WAVE-binding region, central proline-rich motifs, and C-terminal SH3 domain, ABI-1 couples upstream guidance, polarity, and trafficking effectors—including MIG-10/Lamellipodin, UNC-53/NAV2, and the F-BAR protein TOCA-2 directly (acting genetically alongside TOCA-1)—to WVE-1/Arp2/3-mediated branched actin filament assembly, while physically binding and being antagonized by the ABL-1 tyrosine kinase. In vivo, ABI-1 drives embryonic epidermal enclosure, excretory-canal and axon outgrowth, neuronal and distal-tip-cell migration, apoptotic cell engulfment, oocyte clathrin-mediated endocytosis, and Netrin/MIG-10B-dependent presynaptic actin organization and synaptic vesicle clustering.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0001764 neuron migration | IBA GO_REF:0000033 | KEEP AS NON CORE | Summary: IBA annotation from Eumetazoa node PTN000825624 seeded directly by C. elegans abi-1 (WB:WBGene00015146) and mouse Abi2. Reason: Directly supported in C. elegans (PMID:19168673, PMID:23022657), where abi-1 mutants display incomplete ALM/motoneuron migration; retained as non-core consistently across all PTHR10460 reviews because neuron migration is a tissue-specific developmental outcome of core WRC/adaptor function. Propagation Review Root cause: NO FAILURE NON CORE Sources checked: PANTHER:PTN000825624 · Eumetazoa ABI node SUPPORTS TRANSFER Self-seed WB:WBGene00015146 Supporting Evidence: PMID:19168673 ABI-1 and UNC-53 have an overlapping expression pattern, and display similar cell migration phenotypes in the excretory cell, and in mechanosensory and motoneurons. PMID:23022657 Further analysis using a co-immunoprecipitation system confirmed the interaction of MIG-10 and ABI-1 and showed that it requires the SH3 domain of ABI-1. Single mutants for mig-10 and abi-1 displayed similar phenotypes of incomplete migration of the ALM neurons and truncated outgrowth of the excretory cell canals, suggesting that the ABI-1/MIG-10 interaction is relevant in vivo. |
| GO:0001764 neuron migration | IMP PMID:19168673 The cell migration molecule UNC-53/NAV2 is linked to the ARP... | KEEP AS NON CORE | Summary: IMP annotation from PMID:19168673 showing mechanosensory and motoneuron migration defects in abi-1 mutants. Reason: Well-supported developmental phenotype downstream of ABI-1/UNC-53/Arp2/3 actin remodeling; kept as non-core harmonized across PTHR10460. Supporting Evidence: PMID:19168673 ABI-1 and UNC-53 have an overlapping expression pattern, and display similar cell migration phenotypes in the excretory cell, and in mechanosensory and motoneurons. |
| GO:0001764 neuron migration | IMP PMID:23022657 Abelson interactor-1 (ABI-1) interacts with MRL adaptor prot... | KEEP AS NON CORE | Summary: IMP annotation from PMID:23022657 showing incomplete ALM neuron migration in abi-1(tm494) mutants. Reason: Well-supported in vivo neuronal migration phenotype downstream of MIG-10/ABI-1/WRC actin assembly; kept as non-core. Supporting Evidence: PMID:23022657 Further analysis using a co-immunoprecipitation system confirmed the interaction of MIG-10 and ABI-1 and showed that it requires the SH3 domain of ABI-1. Single mutants for mig-10 and abi-1 displayed similar phenotypes of incomplete migration of the ALM neurons and truncated outgrowth of the excretory cell canals, suggesting that the ABI-1/MIG-10 interaction is relevant in vivo. |
| GO:0005515 protein binding | IPI PMID:19168673 The cell migration molecule UNC-53/NAV2 is linked to the ARP... | MODIFY | Summary: IPI annotation from PMID:19168673 showing ABI-1 binding to the N-terminal calponin-homology domain of UNC-53/NAV2 (WB:WBGene00006788). Reason: Bare GO:0005515 is uninformative per the protein-binding policy; ABI-1 acts as a signaling adaptor linking UNC-53 to the WVE-1/Arp2/3 actin-nucleating machinery, warranting GO:0035591 (signaling adaptor activity). Proposed replacements: signaling adaptor activity Supporting Evidence: PMID:19168673 We have identified ABI-1 as a novel molecular partner of UNC-53/NAV2 and have found that a restricted calponin homology (CH) domain of UNC-53 is sufficient to bind ABI-1. |
| GO:0005515 protein binding | IPI PMID:19447967 Shifted Transversal Design smart-pooling for high coverage i... | REMOVE | Summary: High-throughput smart-pooling yeast two-hybrid interaction with VAB-3 (UniProtKB:G5EDS1) in PMID:19447967. Reason: Bare GO:0005515 from a high-throughput yeast two-hybrid pooling screen (PMID:19447967) is uninformative per the protein-binding policy and lacks in vivo functional or domain-specific validation. Supporting Evidence: PMID:19447967 Here we evaluate STD smart-pooling in yeast two-hybrid (Y2H) interactome mapping. |
| GO:0005515 protein binding | IPI PMID:19447967 Shifted Transversal Design smart-pooling for high coverage i... | REMOVE | Summary: High-throughput smart-pooling yeast two-hybrid interaction with PHM-2 (UniProtKB:O62203) in PMID:19447967. Reason: Bare GO:0005515 from a high-throughput yeast two-hybrid pooling screen (PMID:19447967) is uninformative per the protein-binding policy and lacks in vivo functional or domain-specific validation. Supporting Evidence: PMID:19447967 Here we evaluate STD smart-pooling in yeast two-hybrid (Y2H) interactome mapping. |
| GO:0005515 protein binding | IPI PMID:19447967 Shifted Transversal Design smart-pooling for high coverage i... | MODIFY | Summary: Yeast two-hybrid interaction between ABI-1 and the MRL adaptor MIG-10/Lamellipodin (UniProtKB:P34400) in PMID:19447967. Reason: Bare GO:0005515 is uninformative; direct binding of the ABI-1 SH3 domain to MIG-10 is independently validated in vivo to bridge guidance receptors to the WAVE complex (PMID:23022657, PMID:23209429), supporting GO:0035591 (signaling adaptor activity). Proposed replacements: signaling adaptor activity Supporting Evidence: PMID:23022657 Further analysis using a co-immunoprecipitation system confirmed the interaction of MIG-10 and ABI-1 and showed that it requires the SH3 domain of ABI-1. Single mutants for mig-10 and abi-1 displayed similar phenotypes of incomplete migration of the ALM neurons and truncated outgrowth of the excretory cell canals, suggesting that the ABI-1/MIG-10 interaction is relevant in vivo. PMID:23209429 The SH3 domain of ABI-1 binds to MIG-10, and loss of function of either of these proteins causes similar axon guidance defects. Like MIG-10, ABI-1 functions in both the attractive UNC-6 (netrin) pathway and the repulsive SLT-1 (slit) pathway. |
| GO:0005515 protein binding | IPI PMID:19447967 Shifted Transversal Design smart-pooling for high coverage i... | REMOVE | Summary: High-throughput smart-pooling yeast two-hybrid interaction with M04F3.5 (UniProtKB:Q95Y95) in PMID:19447967. Reason: Bare GO:0005515 from a high-throughput yeast two-hybrid pooling screen (PMID:19447967) is uninformative per the protein-binding policy and lacks in vivo functional or domain-specific validation. Supporting Evidence: PMID:19447967 Here we evaluate STD smart-pooling in yeast two-hybrid (Y2H) interactome mapping. |
| GO:0005515 protein binding | IPI PMID:19447967 Shifted Transversal Design smart-pooling for high coverage i... | REMOVE | Summary: High-throughput smart-pooling yeast two-hybrid interaction with F41B4.1 (UniProtKB:Q966J6) in PMID:19447967. Reason: Bare GO:0005515 from a high-throughput yeast two-hybrid pooling screen (PMID:19447967) is uninformative per the protein-binding policy and lacks in vivo functional or domain-specific validation. Supporting Evidence: PMID:19447967 Here we evaluate STD smart-pooling in yeast two-hybrid (Y2H) interactome mapping. |
| GO:0005515 protein binding | IPI PMID:19447967 Shifted Transversal Design smart-pooling for high coverage i... | REMOVE | Summary: High-throughput smart-pooling yeast two-hybrid interaction with M57.1 (UniProtKB:Q9TYX9) in PMID:19447967. Reason: Bare GO:0005515 from a high-throughput yeast two-hybrid pooling screen (PMID:19447967) is uninformative per the protein-binding policy and lacks in vivo functional or domain-specific validation. Supporting Evidence: PMID:19447967 Here we evaluate STD smart-pooling in yeast two-hybrid (Y2H) interactome mapping. |
| GO:0005515 protein binding | IPI PMID:19798448 Requirements for F-BAR proteins TOCA-1 and TOCA-2 in actin d... | MODIFY | Summary: IPI annotation from PMID:19798448 (WormBase) showing ABI-1 direct interaction with F-BAR/SH3 protein TOCA-2 / CeTOCA-2 (WB:WBGene00010663 = UniProtKB:Q9XUS7; distinct from toca-1 WB:WBGene00017298). Reason: Bare GO:0005515 is uninformative; WB:WBGene00010663 is C. elegans toca-2 (CeTOCA-2, UniProtKB:Q9XUS7 in PANTHER PTHR15735:SF16, distinct from toca-1 WB:WBGene00017298 / UniProtKB:Q19253), whose C-terminal SH3 domain binds in vitro translated C. elegans ABI-1 directly (Figure 7C; the same interaction partner curated by UniProt as GO:0017124 on UniProtKB:Q9XUS7), warranting refinement to GO:0017124 (SH3 domain binding). Proposed replacements: SH3 domain binding Supporting Evidence: PMID:19798448 In keeping with this notion, we found that in vitro translated C. elegans ABI-1 readily interacted with the SH3 domain of CeTOCA-2 (Figure 7C), indicating that this interaction is conserved in the nematode. |
| GO:0005515 protein binding | IPI PMID:23022657 Abelson interactor-1 (ABI-1) interacts with MRL adaptor prot... | MODIFY | Summary: IPI annotation from PMID:23022657 showing co-immunoprecipitation of ABI-1 with MIG-10 (WB:WBGene00003243) via the ABI-1 SH3 domain. Reason: Bare GO:0005515 is uninformative; ABI-1 functions as a signaling adaptor whose C-terminal SH3 domain binds MIG-10 to couple guidance signaling to the WVE-1 complex, warranting GO:0035591 (signaling adaptor activity). Proposed replacements: signaling adaptor activity Supporting Evidence: PMID:23022657 Further analysis using a co-immunoprecipitation system confirmed the interaction of MIG-10 and ABI-1 and showed that it requires the SH3 domain of ABI-1. Single mutants for mig-10 and abi-1 displayed similar phenotypes of incomplete migration of the ALM neurons and truncated outgrowth of the excretory cell canals, suggesting that the ABI-1/MIG-10 interaction is relevant in vivo. PMID:23209429 The SH3 domain of ABI-1 binds to MIG-10, and loss of function of either of these proteins causes similar axon guidance defects. Like MIG-10, ABI-1 functions in both the attractive UNC-6 (netrin) pathway and the repulsive SLT-1 (slit) pathway. |
| GO:0005515 protein binding | IPI PMID:23549480 SH3 interactome conserves general function over specific for... | REMOVE | Summary: High-throughput C. elegans SH3 yeast two-hybrid interactome hit with PHM-2 (UniProtKB:O62203) in PMID:23549480. Reason: Bare GO:0005515 from a high-throughput yeast two-hybrid screen (PMID:23549480) is uninformative per the protein-binding policy and lacks physiological validation. Supporting Evidence: PMID:23549480 We then mapped the worm SH3 interactome using stringent yeast two-hybrid and compared it with the equivalent map for yeast. |
| GO:0005515 protein binding | IPI PMID:23549480 SH3 interactome conserves general function over specific for... | MODIFY | Summary: SH3 yeast two-hybrid interaction between ABI-1 and MIG-10 (UniProtKB:P34400) in PMID:23549480. Reason: Bare GO:0005515 is uninformative; while PMID:23549480's cached main text documents ABI-1 SH3#1 PxxP motif binding (PPPTPPRKPNI on B0303.7), the specific ABI-1–MIG-10 binary Y2H pair comes from PMID:23549480's supplementary worm SH3 interactome dataset rather than the main text and is validated physiologically in axon guidance, cell migration, and presynaptic actin organization (PMID:23022657, PMID:23028145), supporting GO:0035591 (signaling adaptor activity). Proposed replacements: signaling adaptor activity Supporting Evidence: PMID:23549480 One example of such a potentially coincident PPI involves ABI-1 SH3#1 and NCK-1 SH3#3, which both bind to B0303.7 with different specificities at two distinct binding regions: PPPTPPRKPNI (amino-acid residues 5–15) for ABI-1#1 and PPVPRH (residues 174–179) for NCK-1#3 (Supplementary Figure 7A). PMID:23022657 Further analysis using a co-immunoprecipitation system confirmed the interaction of MIG-10 and ABI-1 and showed that it requires the SH3 domain of ABI-1. Single mutants for mig-10 and abi-1 displayed similar phenotypes of incomplete migration of the ALM neurons and truncated outgrowth of the excretory cell canals, suggesting that the ABI-1/MIG-10 interaction is relevant in vivo. PMID:23028145 MIG-10B interacts with Abl-interacting protein-1 (ABI-1)/Abi1, a component of the WAVE complex, to organize the actin cytoskeleton at presynaptic sites and instruct vesicle clustering through SNN-1/Synapsin. |
| GO:0005515 protein binding | IPI PMID:23549480 SH3 interactome conserves general function over specific for... | REMOVE | Summary: High-throughput C. elegans SH3 yeast two-hybrid interactome hit with M04F3.5 (UniProtKB:Q95Y95) in PMID:23549480. Reason: Bare GO:0005515 from a high-throughput yeast two-hybrid screen (PMID:23549480) is uninformative per the protein-binding policy and lacks physiological validation. Supporting Evidence: PMID:23549480 We then mapped the worm SH3 interactome using stringent yeast two-hybrid and compared it with the equivalent map for yeast. |
| GO:0005515 protein binding | IPI PMID:23549480 SH3 interactome conserves general function over specific for... | REMOVE | Summary: High-throughput C. elegans SH3 yeast two-hybrid interactome hit with F41B4.1 (UniProtKB:Q966J6) in PMID:23549480. Reason: Bare GO:0005515 from a high-throughput yeast two-hybrid screen (PMID:23549480) is uninformative per the protein-binding policy and lacks physiological validation. Supporting Evidence: PMID:23549480 We then mapped the worm SH3 interactome using stringent yeast two-hybrid and compared it with the equivalent map for yeast. |
| GO:0005737 cytoplasm | IDA PMID:19168673 The cell migration molecule UNC-53/NAV2 is linked to the ARP... | ACCEPT | Summary: WormBase IDA annotation from PMID:19168673 (abstract-only in local cache) for cytoplasmic localization of C. elegans ABI-1, reinforced by cytoplasmic WRC residence. Reason: PMID:19168673 is abstract-only (full_text_available: false) in the local cache, where its abstract reports overlapping abi-1 and unc-53 expression and shared excretory-cell and neuronal migration phenotypes rather than fluorescence microscopy; ACCEPT defers to the WormBase curator's full-text IDA evaluation of PMID:19168673, reinforced by C. elegans ABI-1's established identity as a cytoplasmic protein and obligate WAVE regulatory complex subunit (PMID:23022657, PMID:23028145). Supporting Evidence: PMID:23022657 Each of the cytoplasmic proteins MIG-10/Lpd and ABI-1 has previously been shown to promote cell migration and process outgrowth in nematodes and vertebrates, but a direct interaction between these molecules has not been reported previously. PMID:23028145 MIG-10B interacts with Abl-interacting protein-1 (ABI-1)/Abi1, a component of the WAVE complex, to organize the actin cytoskeleton at presynaptic sites and instruct vesicle clustering through SNN-1/Synapsin. |
| GO:0005856 cytoskeleton | IEA GO_REF:0000044 | ACCEPT | Summary: UniProt subcellular-location mapping to the cytoskeleton, accepted at the broad organelle/network level for a dynamically recruited cytoskeletal regulatory adaptor. Reason: Broad GO:0005856 is deliberately accepted as-is because ABI-1 is a cytoplasmic signaling adaptor and WVE-1/WAVE complex subunit dynamically recruited across multiple cytoskeletal remodeling sites via UNC-53/NAV2 and MIG-10 rather than an intrinsic structural subunit of a single filament polymer (PMID:19168673, PMID:23028145). Supporting Evidence: PMID:19168673 We have identified ABI-1 as a novel molecular partner of UNC-53/NAV2 and have found that a restricted calponin homology (CH) domain of UNC-53 is sufficient to bind ABI-1. PMID:23028145 MIG-10B interacts with Abl-interacting protein-1 (ABI-1)/Abi1, a component of the WAVE complex, to organize the actin cytoskeleton at presynaptic sites and instruct vesicle clustering through SNN-1/Synapsin. |
| GO:0016477 cell migration | IGI PMID:19402756 Abl kinase inhibits the engulfment of apoptotic [corrected] ... | ACCEPT | Summary: IGI annotation from PMID:19402756 demonstrating that abi-1 acts with ced-10/Rac in distal tip cell migration. Reason: Core conserved biological process driven by the C. elegans WVE-1/ABI-1 complex during epidermal, excretory, distal tip cell, and neuronal migrations (PMID:19168673, PMID:19402756). Supporting Evidence: PMID:19402756 We found that ABI-1 the C. elegans homolog of mammalian Abi (Abl interactor) proteins, is required for engulfment of apoptotic cells and proper DTC migration. PMID:19168673 ABI-1 and UNC-53 have an overlapping expression pattern, and display similar cell migration phenotypes in the excretory cell, and in mechanosensory and motoneurons. |
| GO:0017124 SH3 domain binding | IPI PMID:19798448 Requirements for F-BAR proteins TOCA-1 and TOCA-2 in actin d... | ACCEPT | Summary: IPI annotation from PMID:19798448 showing ABI-1 binding to the SH3 domain of TOCA-2 (UniProtKB:Q9XUS7). Reason: Direct biochemical evidence in PMID:19798448 demonstrating conserved binding of C. elegans ABI-1 to the SH3 domain of CeTOCA-2. Supporting Evidence: PMID:19798448 In keeping with this notion, we found that in vitro translated C. elegans ABI-1 readily interacted with the SH3 domain of CeTOCA-2 (Figure 7C), indicating that this interaction is conserved in the nematode. |
| GO:0019901 protein kinase binding | IPI PMID:19402756 Abl kinase inhibits the engulfment of apoptotic [corrected] ... | ACCEPT | Summary: IPI annotation from PMID:19402756 demonstrating physical interaction in vitro between C. elegans ABI-1 and ABL-1 tyrosine kinase (WB:WBGene00000018). Reason: Establishes deep bilaterian conservation of direct Abl tyrosine kinase binding by the sole invertebrate ABI ortholog. Supporting Evidence: PMID:19402756 ABL-1 and ABI-1 interact physically in vitro. We propose that ABL-1 opposes the engulfment of apoptotic cells by inhibiting ABI-1 via a pathway that is distinct from the two known engulfment pathways. |
| GO:0030027 lamellipodium | IBA GO_REF:0000033 | ACCEPT | Summary: IBA annotation from PTN000048906 for active localization at the lamellipodium. Reason: Conserved core localization of the WVE-1/ABI-1 WAVE regulatory complex at protrusive leading edges (PMID:19798448, PMID:23209429). Propagation Review Root cause: NO FAILURE CORE Sources checked: PANTHER:PTN000048906 · Ancestral ABI family node SUPPORTS TRANSFER Conserved WRC localization across Metazoa Supporting Evidence: PMID:19798448 loss of CeTOCA proteins reduced the efficiency of Clathrin-mediated endocytosis (CME) in oocytes. Genetic interference with CeTOCAs interacting proteins WSP-1 and WVE-1, and other components of the WVE-1 complex, produced a similar effect. PMID:23209429 The SH3 domain of ABI-1 binds to MIG-10, and loss of function of either of these proteins causes similar axon guidance defects. Like MIG-10, ABI-1 functions in both the attractive UNC-6 (netrin) pathway and the repulsive SLT-1 (slit) pathway. |
| GO:0030027 lamellipodium | IEA GO_REF:0000044 | ACCEPT | Summary: UniProt subcellular-location mapping to the lamellipodium. Reason: Consistent with conserved WRC localization and IBA from PTN000048906. Supporting Evidence: PMID:23209429 The SH3 domain of ABI-1 binds to MIG-10, and loss of function of either of these proteins causes similar axon guidance defects. Like MIG-10, ABI-1 functions in both the attractive UNC-6 (netrin) pathway and the repulsive SLT-1 (slit) pathway. |
| GO:0030424 axon | IDA PMID:19168673 The cell migration molecule UNC-53/NAV2 is linked to the ARP... | KEEP AS NON CORE | Summary: WormBase IDA annotation from PMID:19168673 (abstract-only in local cache) for ABI-1 localization to C. elegans axons. Reason: PMID:19168673 is abstract-only (full_text_available: false) in the local cache and establishes ABI-1 co-expression and shared migration/outgrowth phenotypes with UNC-53/NAV2 in mechanosensory and motoneurons rather than microscopy details; deferring to the WormBase curator's full-text IDA evaluation of PMID:19168673 (reinforced by MIG-10B-ABI-1 presynaptic actin organization and axon guidance evidence in PMID:23028145 and PMID:23209429), this neuronal axonal compartment is retained as non-core. Supporting Evidence: PMID:19168673 ABI-1 and UNC-53 have an overlapping expression pattern, and display similar cell migration phenotypes in the excretory cell, and in mechanosensory and motoneurons. PMID:23028145 MIG-10B interacts with Abl-interacting protein-1 (ABI-1)/Abi1, a component of the WAVE complex, to organize the actin cytoskeleton at presynaptic sites and instruct vesicle clustering through SNN-1/Synapsin. PMID:23209429 The SH3 domain of ABI-1 binds to MIG-10, and loss of function of either of these proteins causes similar axon guidance defects. Like MIG-10, ABI-1 functions in both the attractive UNC-6 (netrin) pathway and the repulsive SLT-1 (slit) pathway. |
| GO:0030425 dendrite | IDA PMID:19168673 The cell migration molecule UNC-53/NAV2 is linked to the ARP... | KEEP AS NON CORE | Summary: WormBase IDA annotation from PMID:19168673 (abstract-only in local cache) for ABI-1 localization to C. elegans dendrites. Reason: PMID:19168673 is abstract-only (full_text_available: false) in the local cache and reports overlapping neuronal expression and migration phenotypes of ABI-1 and UNC-53/NAV2 in mechanosensory and motoneurons rather than subcellular microscopy; deferring to the WormBase curator's full-text IDA evaluation of PMID:19168673, this specialized dendritic compartment is retained as non-core. Supporting Evidence: PMID:19168673 ABI-1 and UNC-53 have an overlapping expression pattern, and display similar cell migration phenotypes in the excretory cell, and in mechanosensory and motoneurons. |
| GO:0031209 SCAR complex | IBA GO_REF:0000033 | ACCEPT | Summary: IBA annotation from PTN000048906 for membership in the SCAR/WAVE regulatory complex. Reason: ABI-1 is the sole C. elegans ABI subunit of the WVE-1/GEX-2/GEX-3/ABI-1/BRK-1 WAVE complex (PMID:19798448, PMID:23028145). Propagation Review Root cause: NO FAILURE CORE Sources checked: PANTHER:PTN000048906 · Ancestral ABI family node SUPPORTS TRANSFER Single-copy C. elegans ortholog in conserved WRC Supporting Evidence: PMID:19798448 loss of CeTOCA proteins reduced the efficiency of Clathrin-mediated endocytosis (CME) in oocytes. Genetic interference with CeTOCAs interacting proteins WSP-1 and WVE-1, and other components of the WVE-1 complex, produced a similar effect. PMID:23028145 MIG-10B interacts with Abl-interacting protein-1 (ABI-1)/Abi1, a component of the WAVE complex, to organize the actin cytoskeleton at presynaptic sites and instruct vesicle clustering through SNN-1/Synapsin. |
| GO:0035591 signaling adaptor activity | IBA GO_REF:0000033 | ACCEPT | Summary: IBA annotation from PTN000048906 for signaling adaptor activity. Reason: Core molecular function of ABI-1 coupling UNC-53, MIG-10, TOCA-1/2, and ABL-1 to the WVE-1/Arp2/3 actin nucleation machinery. Propagation Review Root cause: NO FAILURE CORE Sources checked: PANTHER:PTN000048906 · Ancestral ABI family node SUPPORTS TRANSFER Conserved WRC/SH3 adaptor activity Supporting Evidence: PMID:19168673 We have identified ABI-1 as a novel molecular partner of UNC-53/NAV2 and have found that a restricted calponin homology (CH) domain of UNC-53 is sufficient to bind ABI-1. PMID:23022657 Further analysis using a co-immunoprecipitation system confirmed the interaction of MIG-10 and ABI-1 and showed that it requires the SH3 domain of ABI-1. Single mutants for mig-10 and abi-1 displayed similar phenotypes of incomplete migration of the ALM neurons and truncated outgrowth of the excretory cell canals, suggesting that the ABI-1/MIG-10 interaction is relevant in vivo. PMID:23209429 The SH3 domain of ABI-1 binds to MIG-10, and loss of function of either of these proteins causes similar axon guidance defects. Like MIG-10, ABI-1 functions in both the attractive UNC-6 (netrin) pathway and the repulsive SLT-1 (slit) pathway. |
| GO:0043025 neuronal cell body | IDA PMID:19168673 The cell migration molecule UNC-53/NAV2 is linked to the ARP... | KEEP AS NON CORE | Summary: WormBase IDA annotation from PMID:19168673 (abstract-only in local cache) for ABI-1 localization to C. elegans neuronal cell bodies. Reason: PMID:19168673 is abstract-only (full_text_available: false) in the local cache and establishes neuronal expression of ABI-1 alongside UNC-53/NAV2 in mechanosensory and motoneurons rather than subcellular microscopy; deferring to the WormBase curator's full-text IDA evaluation of PMID:19168673, this tissue-specific neuronal soma localization is retained as non-core. Supporting Evidence: PMID:19168673 ABI-1 and UNC-53 have an overlapping expression pattern, and display similar cell migration phenotypes in the excretory cell, and in mechanosensory and motoneurons. |
| GO:0048858 cell projection morphogenesis | IGI PMID:23022657 Abelson interactor-1 (ABI-1) interacts with MRL adaptor prot... | ACCEPT | Summary: Experimental IGI and IMP annotations from PMID:23022657 demonstrating that ABI-1 and MIG-10 drive excretory-canal and axonal process outgrowth. Reason: Core organismal manifestation of ABI-1/WRC-mediated branched actin assembly in cellular projections (direct PAINT seed for Bilateria node PTN000048907). Supporting Evidence: PMID:23022657 Further analysis using a co-immunoprecipitation system confirmed the interaction of MIG-10 and ABI-1 and showed that it requires the SH3 domain of ABI-1. Single mutants for mig-10 and abi-1 displayed similar phenotypes of incomplete migration of the ALM neurons and truncated outgrowth of the excretory cell canals, suggesting that the ABI-1/MIG-10 interaction is relevant in vivo. PMID:23209429 The SH3 domain of ABI-1 binds to MIG-10, and loss of function of either of these proteins causes similar axon guidance defects. Like MIG-10, ABI-1 functions in both the attractive UNC-6 (netrin) pathway and the repulsive SLT-1 (slit) pathway. |
| GO:0048858 cell projection morphogenesis | IMP PMID:23022657 Abelson interactor-1 (ABI-1) interacts with MRL adaptor prot... | ACCEPT | Summary: Experimental IGI and IMP annotations from PMID:23022657 demonstrating that ABI-1 and MIG-10 drive excretory-canal and axonal process outgrowth. Reason: Core organismal manifestation of ABI-1/WRC-mediated branched actin assembly in cellular projections (direct PAINT seed for Bilateria node PTN000048907). Supporting Evidence: PMID:23022657 Further analysis using a co-immunoprecipitation system confirmed the interaction of MIG-10 and ABI-1 and showed that it requires the SH3 domain of ABI-1. Single mutants for mig-10 and abi-1 displayed similar phenotypes of incomplete migration of the ALM neurons and truncated outgrowth of the excretory cell canals, suggesting that the ABI-1/MIG-10 interaction is relevant in vivo. PMID:23209429 The SH3 domain of ABI-1 binds to MIG-10, and loss of function of either of these proteins causes similar axon guidance defects. Like MIG-10, ABI-1 functions in both the attractive UNC-6 (netrin) pathway and the repulsive SLT-1 (slit) pathway. |
| GO:0097091 synaptic vesicle clustering | IDA PMID:23028145 Synaptic vesicle clustering requires a distinct MIG-10/Lamel... | KEEP AS NON CORE | Summary: IDA and IMP annotations from PMID:23028145 showing that MIG-10B recruits ABI-1/WRC to instruct synaptic vesicle clustering via SNN-1/Synapsin. Reason: Well-supported specialized presynaptic deployment of ABI-1/WRC downstream of Netrin; kept as non-core. Supporting Evidence: PMID:23028145 MIG-10B interacts with Abl-interacting protein-1 (ABI-1)/Abi1, a component of the WAVE complex, to organize the actin cytoskeleton at presynaptic sites and instruct vesicle clustering through SNN-1/Synapsin. |
| GO:0097091 synaptic vesicle clustering | IMP PMID:23028145 Synaptic vesicle clustering requires a distinct MIG-10/Lamel... | KEEP AS NON CORE | Summary: IDA and IMP annotations from PMID:23028145 showing that MIG-10B recruits ABI-1/WRC to instruct synaptic vesicle clustering via SNN-1/Synapsin. Reason: Well-supported specialized presynaptic deployment of ABI-1/WRC downstream of Netrin; kept as non-core. Supporting Evidence: PMID:23028145 MIG-10B interacts with Abl-interacting protein-1 (ABI-1)/Abi1, a component of the WAVE complex, to organize the actin cytoskeleton at presynaptic sites and instruct vesicle clustering through SNN-1/Synapsin. |
| GO:0098793 presynapse | IEA GO_REF:0000108 | KEEP AS NON CORE | Summary: Inter-ontology IEA inference to presynapse from synaptic vesicle clustering (GO:0097091). Reason: Supported by MIG-10B/ABI-1 localization and actin organization at presynaptic sites in AIY interneurons (PMID:23028145); non-core neuronal compartment. Supporting Evidence: PMID:23028145 MIG-10B interacts with Abl-interacting protein-1 (ABI-1)/Abi1, a component of the WAVE complex, to organize the actin cytoskeleton at presynaptic sites and instruct vesicle clustering through SNN-1/Synapsin. |
| GO:0098858 actin-based cell projection | IBA GO_REF:0000033 | ACCEPT | Summary: IBA annotation from PTN000048906 for active localization in actin-based cell projections. Reason: Core conserved localization of ABI-1 at actin-driven excretory canal and axonal projections (PMID:23022657, PMID:23209429). Propagation Review Root cause: NO FAILURE CORE Sources checked: PANTHER:PTN000048906 · Ancestral ABI family node SUPPORTS TRANSFER Conserved WRC projection localization Supporting Evidence: PMID:23022657 Single mutants for mig-10 and abi-1 displayed similar phenotypes of incomplete migration of the ALM neurons and truncated outgrowth of the excretory cell canals, suggesting that the ABI-1/MIG-10 interaction is relevant in vivo. PMID:23209429 The SH3 domain of ABI-1 binds to MIG-10, and loss of function of either of these proteins causes similar axon guidance defects. Like MIG-10, ABI-1 functions in both the attractive UNC-6 (netrin) pathway and the repulsive SLT-1 (slit) pathway. |
| GO:0099140 presynaptic actin cytoskeleton organization | IDA PMID:23028145 Synaptic vesicle clustering requires a distinct MIG-10/Lamel... | KEEP AS NON CORE | Summary: IDA and IMP annotations from PMID:23028145 showing ABI-1 requirement for presynaptic F-actin organization. Reason: Direct cell-type-specific manifestation of ABI-1/WRC actin assembly at presynaptic terminals; kept as non-core. Supporting Evidence: PMID:23028145 MIG-10B interacts with Abl-interacting protein-1 (ABI-1)/Abi1, a component of the WAVE complex, to organize the actin cytoskeleton at presynaptic sites and instruct vesicle clustering through SNN-1/Synapsin. |
| GO:0099140 presynaptic actin cytoskeleton organization | IMP PMID:23028145 Synaptic vesicle clustering requires a distinct MIG-10/Lamel... | KEEP AS NON CORE | Summary: IDA and IMP annotations from PMID:23028145 showing ABI-1 requirement for presynaptic F-actin organization. Reason: Direct cell-type-specific manifestation of ABI-1/WRC actin assembly at presynaptic terminals; kept as non-core. Supporting Evidence: PMID:23028145 MIG-10B interacts with Abl-interacting protein-1 (ABI-1)/Abi1, a component of the WAVE complex, to organize the actin cytoskeleton at presynaptic sites and instruct vesicle clustering through SNN-1/Synapsin. |
| GO:1901076 positive regulation of engulfment of apoptotic cell | IGI PMID:19402756 Abl kinase inhibits the engulfment of apoptotic [corrected] ... | KEEP AS NON CORE | Summary: IGI annotations from PMID:19402756 showing that abi-1 promotes engulfment of apoptotic cells in parallel to ced-1/ced-6/ced-7 and ced-2/ced-5/ced-12 pathways and is opposed by abl-1. Reason: Well-established actin-driven phagocytic cup role of C. elegans ABI-1; retained as non-core relative to general WRC scaffolding. Supporting Evidence: PMID:19402756 We found that ABI-1 the C. elegans homolog of mammalian Abi (Abl interactor) proteins, is required for engulfment of apoptotic cells and proper DTC migration. PMID:19402756 ABL-1 and ABI-1 interact physically in vitro. We propose that ABL-1 opposes the engulfment of apoptotic cells by inhibiting ABI-1 via a pathway that is distinct from the two known engulfment pathways. |
| GO:1901076 positive regulation of engulfment of apoptotic cell | IGI PMID:19402756 Abl kinase inhibits the engulfment of apoptotic [corrected] ... | KEEP AS NON CORE | Summary: IGI annotations from PMID:19402756 showing that abi-1 promotes engulfment of apoptotic cells in parallel to ced-1/ced-6/ced-7 and ced-2/ced-5/ced-12 pathways and is opposed by abl-1. Reason: Well-established actin-driven phagocytic cup role of C. elegans ABI-1; retained as non-core relative to general WRC scaffolding. Supporting Evidence: PMID:19402756 We found that ABI-1 the C. elegans homolog of mammalian Abi (Abl interactor) proteins, is required for engulfment of apoptotic cells and proper DTC migration. PMID:19402756 ABL-1 and ABI-1 interact physically in vitro. We propose that ABL-1 opposes the engulfment of apoptotic cells by inhibiting ABI-1 via a pathway that is distinct from the two known engulfment pathways. |
| GO:2000370 positive regulation of clathrin-dependent endocytosis | IMP PMID:19798448 Requirements for F-BAR proteins TOCA-1 and TOCA-2 in actin d... | KEEP AS NON CORE | Summary: IMP annotation from PMID:19798448 showing reduced oocyte clathrin-mediated endocytosis upon depletion of WVE-1/ABI-1 complex components interacting with TOCA-1/2. Reason: Supported secondary membrane-trafficking role of ABI-1 bridging TOCA-1/2 to WVE-1; kept as non-core. Supporting Evidence: PMID:19798448 Furthermore, mammalian TOCA-1 and C. elegans CeTOCAs physically associated with N-WASP and WSP-1 directly, or WAVE2 indirectly via ABI-1. PMID:19798448 loss of CeTOCA proteins reduced the efficiency of Clathrin-mediated endocytosis (CME) in oocytes. Genetic interference with CeTOCAs interacting proteins WSP-1 and WVE-1, and other components of the WVE-1 complex, produced a similar effect. |
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Download this section (compressed HTML)Q: Which tyrosine residues on C. elegans ABI-1 or WVE-1 are phosphorylated by ABL-1 in vivo to inhibit apoptotic cell engulfment while permitting axon guidance?
Q: Does the C. elegans ABI-1/GEX-2 interface conserve the WIRS-peptide binding surface used by Drosophila and mammalian WAVE regulatory complexes?
Experiment: Phosphoproteomic mapping and CRISPR non-phosphorylatable/phosphomimetic mutagenesis of endogenous C. elegans abi-1 in abl-1 single and ced-1;abl-1 double mutants.
Experiment: Endogenous affinity purification of GFP::ABI-1 from embryonic epidermis versus adult neurons to compare tissue-specific WVE-1, MIG-10A/B, UNC-53, and TOCA-1/2 stoichiometries.
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