| Aspect | Key findings | Evidence type | Citations |
|---|---|---|---|
| Identity / domains | CGH-1 in *Caenorhabditis elegans* is consistently identified as the ortholog of yeast Dhh1 and metazoan DDX6/RCK, i.e., a DEAD-box ATP-dependent RNA helicase. Structural work indicates the canonical two RecA-like helicase domains, with the RecA2 domain acting as a major partner-binding surface; this matches the UniProt DDX6/DHH1-family assignment. | Comparative annotation, primary genetics, structural modeling | (pqac-00000001, pqac-00000002, pqac-00000003, pqac-00000004) |
| Enzymatic activity | CGH-1 is an ATPase/RNA helicase-family protein rather than a classical metabolic enzyme; its ATPase activity is stimulated by the NTL-1a MIF4G domain in the presence of poly(U) RNA and ATP, consistent with conserved DDX6-family RNA remodeling. Direct worm biochemical data support ATPase function, while recent human DDX6 work strengthens the conserved model that ATPase activity and RecA2-mediated interactions are required for coupling inefficient translation to mRNA decay. | Biochemistry, structural/interaction assays, cross-species mechanistic inference | (pqac-00000003, pqac-00000004, pqac-00000016, pqac-00000019) |
| Localization / condensates | CGH-1 localizes to distinct cytoplasmic RNP condensates depending on context: somatic P bodies, oocyte storage bodies, embryonic/germline P-bodies, and perinuclear P-body condensates adjacent to P granules. Imaging and figure-based spatial analysis show CGH-1 lies predominantly on the cytoplasmic side of P-granule markers and often adjacent to Z granules/Mutator foci, supporting a role at the interface of mRNA regulation and germ-granule organization. | Imaging, cell biology, figure-based spatial quantification | (pqac-00000005, pqac-00000008, pqac-00000010, pqac-00000012, pqac-00000024) |
| Interaction partners | CGH-1 binds core P-body / translational control factors including EDC-3, PATR-1, and CAR-1. Quantitative binding data from structural/ITC analyses show EDC-3 FDF peptide binds CGH-1 RecA2 with KD ~0.34 uM; reported affinities for PATR-1 and CAR-1 are ~2.13 uM and ~3.03 uM, respectively, indicating a conserved interaction hub for decapping and repression factors. | Structural biology, ITC, GST pulldown, co-localization, IP | (pqac-00000002, pqac-00000003, pqac-00000004, pqac-00000005) |
| Biological processes / pathways | In somatic tissues, CGH-1 participates in PATR-1-dependent P bodies linked to decapping-mediated mRNA turnover; during oogenesis, it instead forms PATR-1-independent storage bodies that protect specific maternal mRNAs from degradation. In embryos and adult germlines, CGH-1-containing germline P-bodies contribute to maternal mRNA regulation, germ cell fate specification, and organization of small-RNA silencing condensates around germ granules. | Genetics, imaging, RIP/omics, developmental cell biology | (pqac-00000000, pqac-00000008, pqac-00000009, pqac-00000010, pqac-00000023) |
| Phenotypes / functional outcomes | Loss or perturbation of CGH-1 disrupts maternal mRNA storage/protection, CAR-1 organization, and ER remodeling in arrested oocytes, and affects fertility/developmental outcomes. Quantitatively, 92% of CGH-1-associated mRNAs are gonad-enriched and 85% are maternal; patr-1 loss causes about a 12-fold reduction in somatic CGH-1 foci by the ~100-cell stage, highlighting context-specific condensate assembly. | Genetics, RIP-chip/omics, imaging, developmental phenotyping | (pqac-00000005, pqac-00000015) |
| Recent 2023-2024 developments | New work places CGH-1 at the center of condensate cooperativity in the germline: P bodies containing CGH-1 coat germ granules, help localize PRG-1/CSR-1 and related factors, and are required for efficient maintenance of transgenerational silencing through secondary small-RNA amplification. Broader 2024 DDX6 studies show conserved ATPase- and cofactor-dependent control of P-body assembly, stress-granule docking, and selective repression/decay of inefficiently translated mRNAs; in human cells, DDX6 loss altered 1707 upregulated and 1484 downregulated transcripts, with 260 transcripts showing increased translation efficiency. | Recent primary literature, imaging, IP-MS, FRAP, RNA-seq/ribosome profiling, cross-species mechanistic inference | (pqac-00000010, pqac-00000012, pqac-00000017, pqac-00000018, pqac-00000021, pqac-00000022) |


*Table: This table summarizes key functional annotation evidence for *C. elegans* CGH-1, emphasizing molecular function, localization, interaction partners, pathways, phenotypes, and recent 2023-2024 advances. It is useful as a compact evidence map linking classical worm genetics/cell biology with newer mechanistic and condensate-focused studies.*