| Source (with year) | Publication type | Experimental system | Key findings about *nhr-47* | Evidence type (expression/RNAi/other) | Quantitative details (doses, fold-changes, generations, sample sizes) | URL/DOI |
|---|---|---|---|---|---|---|
| Liu et al., 2024 | Primary research article | *C. elegans* gonads; polystyrene nanoplastic (PS-NP) exposure with germline RNAi and transgenerational assays | *nhr-47* is identified as a germline nuclear hormone receptor whose expression increases after PS-NP exposure; germline *nhr-47*(RNAi) confers resistance to transgenerational toxicity, suppressing PS-NP-induced locomotion and brood-size defects; *nhr-47* RNAi decreases gonadal expression of insulin ligands (*ins-3, ins-39, daf-28*) and *efn-3*, and reduces offspring receptor expression (*daf-2, vab-1*), implicating insulin/Ephrin-associated signaling in the response (pqac-00000001, pqac-00000002, pqac-00000010) | Expression; RNAi; pathway inference | PS-NPs at 0.1, 1, 10 µg/L; *nhr-47* increased in P0 after 1 and 10 µg/L; transgenerational elevation persisted to F2 at 1 µg/L and F4 at 10 µg/L, returning to control by F5 at 10 µg/L; qRT-PCR on isolated gonads with 30 gonads/treatment; Figures 1D, 2, 3, 4 support expression and phenotype claims (pqac-00000001, pqac-00000002, pqac-00000010) | https://doi.org/10.3390/toxics12060420 ; DOI: 10.3390/toxics12060420 |
| Novillo et al., 2005 | Primary research article | *C. elegans* exposed to estradiol; DNA microarray profiling | Exogenous estradiol induces over-expression of *nhr-47*, supporting that *nhr-47* is environmentally/hormonally responsive, but no direct mechanistic or phenotypic role was established in this study (pqac-00000003) | Expression | Reported fold change for *nhr-47* = 3.4 under estradiol treatment; estradiol concentration reported as 10^-5 M in the table/text excerpt; microarray-based mRNA profiling via Stanford Microarray Database (pqac-00000003) | https://doi.org/10.1093/icb/45.1.61 ; DOI: 10.1093/icb/45.1.61 |
| Brožová, 2006 | Thesis/dissertation (methods/results excerpts) | *C. elegans* RNAi and *nhr-40::gfp* reporter strains | *nhr-47* was targeted by dsRNA corresponding to part of its putative ligand-binding domain; in reporter assays, *nhr-47* RNAi did not alter *nhr-40::gfp* expression, suggesting no detectable regulation of *nhr-40* reporter output in that assay; no direct phenotype for *nhr-47* itself was reported in the excerpt (pqac-00000004, pqac-00000006, pqac-00000009) | RNAi; other | 1,040 bp PCR fragment from mixed-stage N2 cDNA; dsRNA concentration ~2–3 µg/µl; assay performed by microinjection into young adult hermaphrodites carrying *nhr-40::gfp* strains #4586 and #4523; result was negative for reporter change (pqac-00000004, pqac-00000006) | No stable URL provided in retrieved evidence |
| Ciche & Sternberg, 2007 | Primary research article | *Heterorhabditis bacteriophora* EST mapping against *C. elegans* homologs; RNAi resource context | An EST annotated as Hba-*nhr-47* was mapped to *C. elegans nhr-47* (C24G6.4.1), confirming orthology/resource linkage, but the retrieved excerpt provides no direct functional, expression, or phenotype data for *C. elegans nhr-47* itself (pqac-00000005, pqac-00000008) | Other | Hba-*nhr-47* EST listed with GenBank accession EE724175; evidence is mapping/alignment-based rather than mechanistic functional analysis (pqac-00000005, pqac-00000008) | https://doi.org/10.1186/1471-213X-7-101 ; DOI: 10.1186/1471-213X-7-101 |
| Pohludka, 2009 | Review/thesis-style functional genomics source | Comparative/domain-based analysis of nematode nuclear receptors | Places NHR-47 within *C. elegans* class I subgroup 8 nuclear hormone receptors characterized by the P-box sequence CNGCKT, consistent with HNF4/NR2-like DNA-binding properties and nuclear receptor domain architecture; does not provide direct *nhr-47*-specific functional assays (pqac-00000000, pqac-00000007) | Other; classification/domain inference | No *nhr-47*-specific quantitative functional data; provides general NHR architecture: conserved DBD with zinc fingers, hinge region linked to nuclear localization, and ligand-binding domain used for transcriptional regulation (pqac-00000000, pqac-00000007) | No stable URL provided in retrieved evidence |


*Table: This table summarizes the retrieved evidence specifically relevant to *Caenorhabditis elegans nhr-47* (Q17370/C24G6.4), distinguishing direct functional data from indirect classification or resource-based evidence. It is useful for separating experimentally supported claims from weaker inferences when annotating this relatively understudied nuclear hormone receptor.*