ADK2 (AKY3/PAK3; YER170W) encodes the mitochondrial matrix GTP:AMP phosphotransferase of S. cerevisiae, the orthologue of vertebrate AK3. It catalyses NTP + AMP -> NDP + ADP using GTP or ITP but not ATP as phosphate donor. A strain-dependent frameshift near the 3' end yields a short (PAK3) or long (AKY3) C-terminus; both forms are active at 30 C, but the long form is more stable to heat and denaturation. Expression is low on glucose and rises on non-fermentable carbon sources. Disruption has no detectable phenotype, so its physiological role in mitochondrial nucleotide homeostasis (e.g. recycling AMP and GTP generated in the matrix) remains undefined.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0004017 AMP kinase activity | IBA GO_REF:0000033 | REMOVE | Summary: IBA from PTN000599576, the adenylate kinase node that also covers ATP-using AK1/AK2 enzymes. Adk2 sits in the AK3 (GTP:AMP) subclade and has target-specific biochemical evidence that ATP is not a phosphate donor, so the ATP:AMP activity is lost/diverged in this lineage. The correct activity is captured by the IBA to nucleoside triphosphate adenylate kinase activity from the AK3 node. Reason: Adk2 (yeast AK3 orthologue) uses GTP or ITP but does not accept ATP as phosphate donor, so ATP:AMP 'AMP kinase activity' is incorrect for this protein. Propagation Review Root cause: PROPAGATION BAD Failure modes: WRONG ORTHOLOG OR PARALOG FUNCTIONAL DIVERGENCE Sources checked: PANTHER:PTN000599576 SUPPORTS SOURCE BUT NOT TARGET Node covers ATP-using AK1/AK2-type adenylate kinases; Adk2 belongs to the AK3 subclade (PTN008598552) that uses GTP/ITP and not ATP. Supporting Evidence: PMID:8537371 The encoded protein exhibited GTP:AMP and ITP:AMP phosphotransferase activities but did not accept ATP as phosphate donor file:yeast/ADK2/ADK2-uniprot.txt Has GTP:AMP phosphotransferase and ITP:AMP phosphotransferase activities. Does not accept ATP as phosphate donor. |
| GO:0004017 AMP kinase activity | IEA GO_REF:0000002 | REMOVE | Summary: IEA from InterPro IPR006259/IPR007862 (adenylate kinase family/lid domain) mapped to AMP kinase activity. The family signature does not discriminate the GTP-specific AK3 subfamily; biochemistry shows Adk2 does not use ATP. Reason: Adk2 (yeast AK3 orthologue) uses GTP or ITP but does not accept ATP as phosphate donor, so ATP:AMP 'AMP kinase activity' is incorrect for this protein. Supporting Evidence: PMID:8537371 The encoded protein exhibited GTP:AMP and ITP:AMP phosphotransferase activities but did not accept ATP as phosphate donor file:yeast/ADK2/ADK2-uniprot.txt Has GTP:AMP phosphotransferase and ITP:AMP phosphotransferase activities. Does not accept ATP as phosphate donor. |
| GO:0004017 AMP kinase activity | RCA GO_REF:0000123 | REMOVE | Summary: RCA from YeastPathways DENOVOPURINE2-PWY (superpathway of purine nucleotides de novo biosynthesis II), where YeastCyc attaches Adk2 to the AMP + ATP <-> 2 ADP reaction alongside Adk1. This is a paralog over-assignment: Adk2 is a matrix GTP:AMP phosphotransferase that does not accept ATP. Reason: Adk2 (yeast AK3 orthologue) uses GTP or ITP but does not accept ATP as phosphate donor, so ATP:AMP 'AMP kinase activity' is incorrect for this protein. Supporting Evidence: PMID:8537371 The encoded protein exhibited GTP:AMP and ITP:AMP phosphotransferase activities but did not accept ATP as phosphate donor file:yeast/ADK2/ADK2-uniprot.txt Has GTP:AMP phosphotransferase and ITP:AMP phosphotransferase activities. Does not accept ATP as phosphate donor. |
| GO:0004017 AMP kinase activity | RCA GO_REF:0000123 | REMOVE | Summary: RCA from YeastPathways DENOVOPURINE3-PWY (de novo biosynthesis of purine nucleotides), where YeastCyc attaches Adk2 to the AMP + ATP <-> 2 ADP reaction alongside Adk1. This is a paralog over-assignment: Adk2 is a matrix GTP:AMP phosphotransferase that does not accept ATP. Reason: Adk2 (yeast AK3 orthologue) uses GTP or ITP but does not accept ATP as phosphate donor, so ATP:AMP 'AMP kinase activity' is incorrect for this protein. Supporting Evidence: PMID:8537371 The encoded protein exhibited GTP:AMP and ITP:AMP phosphotransferase activities but did not accept ATP as phosphate donor file:yeast/ADK2/ADK2-uniprot.txt Has GTP:AMP phosphotransferase and ITP:AMP phosphotransferase activities. Does not accept ATP as phosphate donor. |
| GO:0004017 AMP kinase activity | RCA GO_REF:0000123 | REMOVE | Summary: RCA from YeastPathways PRPP-PWY-1 (superpathway of histidine, purine, and pyrimidine biosynthesis), where YeastCyc attaches Adk2 to the AMP + ATP <-> 2 ADP reaction alongside Adk1. This is a paralog over-assignment: Adk2 is a matrix GTP:AMP phosphotransferase that does not accept ATP. Reason: Adk2 (yeast AK3 orthologue) uses GTP or ITP but does not accept ATP as phosphate donor, so ATP:AMP 'AMP kinase activity' is incorrect for this protein. Supporting Evidence: PMID:8537371 The encoded protein exhibited GTP:AMP and ITP:AMP phosphotransferase activities but did not accept ATP as phosphate donor file:yeast/ADK2/ADK2-uniprot.txt Has GTP:AMP phosphotransferase and ITP:AMP phosphotransferase activities. Does not accept ATP as phosphate donor. |
| GO:0004017 AMP kinase activity | RCA GO_REF:0000123 | REMOVE | Summary: RCA from YeastPathways PWY-6126-1 (superpathway of adenosine nucleotides de novo biosynthesis II), where YeastCyc attaches Adk2 to the AMP + ATP <-> 2 ADP reaction alongside Adk1. This is a paralog over-assignment: Adk2 is a matrix GTP:AMP phosphotransferase that does not accept ATP. Reason: Adk2 (yeast AK3 orthologue) uses GTP or ITP but does not accept ATP as phosphate donor, so ATP:AMP 'AMP kinase activity' is incorrect for this protein. Supporting Evidence: PMID:8537371 The encoded protein exhibited GTP:AMP and ITP:AMP phosphotransferase activities but did not accept ATP as phosphate donor file:yeast/ADK2/ADK2-uniprot.txt Has GTP:AMP phosphotransferase and ITP:AMP phosphotransferase activities. Does not accept ATP as phosphate donor. |
| GO:0004017 AMP kinase activity | RCA GO_REF:0000123 | REMOVE | Summary: RCA from YeastPathways PWY-7219 (adenosine ribonucleotides de novo biosynthesis), where YeastCyc attaches Adk2 to the AMP + ATP <-> 2 ADP reaction alongside Adk1. This is a paralog over-assignment: Adk2 is a matrix GTP:AMP phosphotransferase that does not accept ATP. Reason: Adk2 (yeast AK3 orthologue) uses GTP or ITP but does not accept ATP as phosphate donor, so ATP:AMP 'AMP kinase activity' is incorrect for this protein. Supporting Evidence: PMID:8537371 The encoded protein exhibited GTP:AMP and ITP:AMP phosphotransferase activities but did not accept ATP as phosphate donor file:yeast/ADK2/ADK2-uniprot.txt Has GTP:AMP phosphotransferase and ITP:AMP phosphotransferase activities. Does not accept ATP as phosphate donor. |
| GO:0004017 AMP kinase activity | RCA GO_REF:0000123 | REMOVE | Summary: RCA from YeastPathways PWY3O-285 (superpathway of purine biosynthesis and salvage pathways), where YeastCyc attaches Adk2 to the AMP + ATP <-> 2 ADP reaction alongside Adk1. This is a paralog over-assignment: Adk2 is a matrix GTP:AMP phosphotransferase that does not accept ATP. Reason: Adk2 (yeast AK3 orthologue) uses GTP or ITP but does not accept ATP as phosphate donor, so ATP:AMP 'AMP kinase activity' is incorrect for this protein. Supporting Evidence: PMID:8537371 The encoded protein exhibited GTP:AMP and ITP:AMP phosphotransferase activities but did not accept ATP as phosphate donor file:yeast/ADK2/ADK2-uniprot.txt Has GTP:AMP phosphotransferase and ITP:AMP phosphotransferase activities. Does not accept ATP as phosphate donor. |
| GO:0005524 ATP binding | IEA GO_REF:0000002 | MARK AS OVER ANNOTATED | Summary: IEA from InterPro IPR000850/IPR006259 (adenylate kinase family), inferring ATP binding. Adk2 does not accept ATP as phosphate donor; its nucleotide donor site binds GTP/ITP. ATP binding is a family-level over-generalisation for this AK3 enzyme. Reason: ATP is not a substrate of Adk2; the P-loop binds GTP/ITP. Supporting Evidence: PMID:8537371 The encoded protein exhibited GTP:AMP and ITP:AMP phosphotransferase activities but did not accept ATP as phosphate donor |
| GO:0005737 cytoplasm | IBA GO_REF:0000033 | MODIFY | Summary: IBA (is_active_in cytoplasm) from PTN000599576, the broad adenylate kinase node dominated by cytosolic AK1/AK2 enzymes. Adk2 is located exclusively in the mitochondrial matrix; the matrix is technically within the cytoplasm in GO, but the IBA reflects cytosolic family members, not Adk2. Reason: Adk2 is a mitochondrial matrix protein; cytoplasm (which in GO includes the mitochondria) is too general. Propagation Review Root cause: PROPAGATION BAD Failure modes: COMPARTMENT OR COMPLEX MISMATCH Sources checked: PANTHER:PTN000599576 SUPPORTS SOURCE BUT NOT TARGET Cytoplasmic activity is true of the AK1/AK2-type members of this node but not of the matrix-localised AK3-type Adk2. Proposed replacements: mitochondrial matrix Supporting Evidence: PMID:8537371 was located exclusively in the mitochondrial matrix file:yeast/ADK2/ADK2-uniprot.txt SUBCELLULAR LOCATION: Mitochondrion matrix |
| GO:0005739 mitochondrion | HDA PMID:16823961 Toward the complete yeast mitochondrial proteome: multidimen... | ACCEPT | Summary: HDA from the PROMITO mitochondrial proteome (Reinders et al. 2006). Correct. Supporting Evidence: PMID:16823961 A total of 851 different proteins (PROMITO dataset) were identified PMID:8537371 was located exclusively in the mitochondrial matrix |
| GO:0005739 mitochondrion | IEA GO_REF:0000002 | ACCEPT | Summary: IEA from InterPro IPR028586 (AK3 mitochondrial). Correct. Supporting Evidence: file:yeast/ADK2/ADK2-uniprot.txt SUBCELLULAR LOCATION: Mitochondrion matrix |
| GO:0005743 mitochondrial inner membrane | IDA PMID:15753074 A GTP:AMP phosphotransferase, Adk2p, in Saccharomyces cerevi... | KEEP AS NON CORE | Summary: IDA (PMID:15753074) assigning Adk2 to the mitochondrial inner membrane. Both abstracts describe Adk2 as a matrix protein, so the inner-membrane annotation probably reflects a peripherally associated fraction in subfractionation. Abstract-only caches; defer to curator but treat as non-core. Reason: Experimental but conflicts with the predominant matrix localisation; may reflect membrane-associated pool. Supporting Evidence: PMID:15753074 the corresponding protein exists as either Adk2p (short) or Adk2p (long) in the mitochondrial matrix |
| GO:0005743 mitochondrial inner membrane | IDA PMID:8537371 Strain-dependent occurrence of functional GTP:AMP phosphotra... | KEEP AS NON CORE | Summary: IDA (PMID:8537371) assigning Adk2 to the mitochondrial inner membrane. Both abstracts describe Adk2 as a matrix protein, so the inner-membrane annotation probably reflects a peripherally associated fraction in subfractionation. Abstract-only caches; defer to curator but treat as non-core. Reason: Experimental but conflicts with the predominant matrix localisation; may reflect membrane-associated pool. Supporting Evidence: PMID:8537371 was located exclusively in the mitochondrial matrix |
| GO:0005759 mitochondrial matrix | IBA GO_REF:0000033 | ACCEPT | Summary: IBA (is_active_in) from PTN008598552 (AK3 clade). Correct; core location. Supporting Evidence: file:yeast/ADK2/ADK2-uniprot.txt SUBCELLULAR LOCATION: Mitochondrion matrix |
| GO:0005759 mitochondrial matrix | IDA PMID:8537371 Strain-dependent occurrence of functional GTP:AMP phosphotra... | ACCEPT | Summary: IDA (Schricker et al. 1995): Adk2 protein was located exclusively in the mitochondrial matrix. Core location. Supporting Evidence: PMID:8537371 was located exclusively in the mitochondrial matrix |
| GO:0005759 mitochondrial matrix | IDA PMID:8537371 Strain-dependent occurrence of functional GTP:AMP phosphotra... | ACCEPT | Summary: IDA (Schricker et al. 1995): Adk2 protein was located exclusively in the mitochondrial matrix. Core location. Supporting Evidence: PMID:8537371 was located exclusively in the mitochondrial matrix |
| GO:0005759 mitochondrial matrix | IEA GO_REF:0000120 | ACCEPT | Summary: IEA from UniProt subcellular location SL-0170 / HAMAP MF_03169. Correct. Supporting Evidence: file:yeast/ADK2/ADK2-uniprot.txt SUBCELLULAR LOCATION: Mitochondrion matrix |
| GO:0005829 cytosol | RCA GO_REF:0000123 | REMOVE | Summary: RCA (is_active_in cytosol) from YeastPathways DENOVOPURINE2-PWY (superpathway of purine nucleotides de novo biosynthesis II), which places all adenylate kinase reaction participants in the cytosol. Adk2 is exclusively mitochondrial (matrix). Reason: Adk2 is a mitochondrial matrix protein; cytosol is inherited from a pathway built around Adk1. Supporting Evidence: PMID:8537371 was located exclusively in the mitochondrial matrix file:yeast/ADK2/ADK2-uniprot.txt SUBCELLULAR LOCATION: Mitochondrion matrix |
| GO:0005829 cytosol | RCA GO_REF:0000123 | REMOVE | Summary: RCA (is_active_in cytosol) from YeastPathways DENOVOPURINE3-PWY (de novo biosynthesis of purine nucleotides), which places all adenylate kinase reaction participants in the cytosol. Adk2 is exclusively mitochondrial (matrix). Reason: Adk2 is a mitochondrial matrix protein; cytosol is inherited from a pathway built around Adk1. Supporting Evidence: PMID:8537371 was located exclusively in the mitochondrial matrix file:yeast/ADK2/ADK2-uniprot.txt SUBCELLULAR LOCATION: Mitochondrion matrix |
| GO:0005829 cytosol | RCA GO_REF:0000123 | REMOVE | Summary: RCA (is_active_in cytosol) from YeastPathways PRPP-PWY-1 (superpathway of histidine, purine, and pyrimidine biosynthesis), which places all adenylate kinase reaction participants in the cytosol. Adk2 is exclusively mitochondrial (matrix). Reason: Adk2 is a mitochondrial matrix protein; cytosol is inherited from a pathway built around Adk1. Supporting Evidence: PMID:8537371 was located exclusively in the mitochondrial matrix file:yeast/ADK2/ADK2-uniprot.txt SUBCELLULAR LOCATION: Mitochondrion matrix |
| GO:0005829 cytosol | RCA GO_REF:0000123 | REMOVE | Summary: RCA (is_active_in cytosol) from YeastPathways PWY-6126-1 (superpathway of adenosine nucleotides de novo biosynthesis II), which places all adenylate kinase reaction participants in the cytosol. Adk2 is exclusively mitochondrial (matrix). Reason: Adk2 is a mitochondrial matrix protein; cytosol is inherited from a pathway built around Adk1. Supporting Evidence: PMID:8537371 was located exclusively in the mitochondrial matrix file:yeast/ADK2/ADK2-uniprot.txt SUBCELLULAR LOCATION: Mitochondrion matrix |
| GO:0005829 cytosol | RCA GO_REF:0000123 | REMOVE | Summary: RCA (is_active_in cytosol) from YeastPathways PWY-7219 (adenosine ribonucleotides de novo biosynthesis), which places all adenylate kinase reaction participants in the cytosol. Adk2 is exclusively mitochondrial (matrix). Reason: Adk2 is a mitochondrial matrix protein; cytosol is inherited from a pathway built around Adk1. Supporting Evidence: PMID:8537371 was located exclusively in the mitochondrial matrix file:yeast/ADK2/ADK2-uniprot.txt SUBCELLULAR LOCATION: Mitochondrion matrix |
| GO:0005829 cytosol | RCA GO_REF:0000123 | REMOVE | Summary: RCA (is_active_in cytosol) from YeastPathways PWY3O-285 (superpathway of purine biosynthesis and salvage pathways), which places all adenylate kinase reaction participants in the cytosol. Adk2 is exclusively mitochondrial (matrix). Reason: Adk2 is a mitochondrial matrix protein; cytosol is inherited from a pathway built around Adk1. Supporting Evidence: PMID:8537371 was located exclusively in the mitochondrial matrix file:yeast/ADK2/ADK2-uniprot.txt SUBCELLULAR LOCATION: Mitochondrion matrix |
| GO:0006164 purine nucleotide biosynthetic process | RCA GO_REF:0000123 | MARK AS OVER ANNOTATED | Summary: RCA from YeastPathways DENOVOPURINE2-PWY (superpathway of purine nucleotides de novo biosynthesis II), which attaches Adk2 to the cytosolic ATP:AMP step. Adk2 is a matrix GTP:AMP phosphotransferase whose loss has no phenotype; there is no evidence that it contributes to purine nucleotide biosynthesis. Reason: Adk2 is dispensable and acts on GTP:AMP in the matrix, not in de novo purine synthesis. Supporting Evidence: PMID:8537371 yeast mitochondria can completely dispense with GTP:AMP phosphotransferase activity PMID:8537371 The encoded protein exhibited GTP:AMP and ITP:AMP phosphotransferase activities but did not accept ATP as phosphate donor |
| GO:0006164 purine nucleotide biosynthetic process | RCA GO_REF:0000123 | MARK AS OVER ANNOTATED | Summary: RCA from YeastPathways DENOVOPURINE3-PWY (de novo biosynthesis of purine nucleotides), which attaches Adk2 to the cytosolic ATP:AMP step. Adk2 is a matrix GTP:AMP phosphotransferase whose loss has no phenotype; there is no evidence that it contributes to purine nucleotide biosynthesis. Reason: Adk2 is dispensable and acts on GTP:AMP in the matrix, not in de novo purine synthesis. Supporting Evidence: PMID:8537371 yeast mitochondria can completely dispense with GTP:AMP phosphotransferase activity PMID:8537371 The encoded protein exhibited GTP:AMP and ITP:AMP phosphotransferase activities but did not accept ATP as phosphate donor |
| GO:0006172 ADP biosynthetic process | IEA GO_REF:0000104 | KEEP AS NON CORE | Summary: IEA from HAMAP MF_03169. ADP is a direct product of the GTP:AMP phosphotransferase reaction, so the term is chemically correct, though the physiological importance is unclear (null has no phenotype). Reason: Correct product-level BP, but the enzyme is dispensable. Supporting Evidence: file:yeast/ADK2/ADK2-uniprot.txt Reaction=a ribonucleoside 5'-triphosphate + AMP = a ribonucleoside 5'- PMID:8537371 disruption of the AKY3 allele displayed no phenotype |
| GO:0009117 nucleotide metabolic process | IDA PMID:15753074 A GTP:AMP phosphotransferase, Adk2p, in Saccharomyces cerevi... | ACCEPT | Summary: IDA (Gu et al. 2005): Adk2 is an active nucleoside phosphate interconverting enzyme. Correct general BP. Supporting Evidence: PMID:15753074 Adenylate kinases participate in maintaining the homeostasis of cellular nucleotides PMID:15753074 both short and long forms of Adk2p are enzymatically active |
| GO:0009117 nucleotide metabolic process | IEA GO_REF:0000117 | ACCEPT | Summary: IEA from ARBA00027584. Correct general BP. Supporting Evidence: file:yeast/ADK2/ADK2-uniprot.txt Has GTP:AMP phosphotransferase and ITP:AMP phosphotransferase activities. Does not accept ATP as phosphate donor. |
| GO:0009142 nucleoside triphosphate biosynthetic process | IBA GO_REF:0000033 | MARK AS OVER ANNOTATED | Summary: IBA from PTN008598552 (AK3 clade). The GTP:AMP phosphotransferase reaction converts GTP + AMP to GDP + ADP, i.e. consumes rather than synthesises a nucleoside triphosphate. Any NTP formation would be via other enzymes regenerating GTP/ATP from the products. Reason: The reaction consumes an NTP; it does not synthesise one. Propagation Review Root cause: TERM SCOPING PROBLEM Failure modes: ROLE CONFLATION Sources checked: PANTHER:PTN008598552 SOURCE WEAK OR INFERRED The AK3 node correctly carries GTP:AMP phosphotransferase activity, but NTP biosynthesis conflates the NTP-consuming reaction with NTP synthesis. Supporting Evidence: file:yeast/ADK2/ADK2-uniprot.txt Reaction=a ribonucleoside 5'-triphosphate + AMP = a ribonucleoside 5'- |
| GO:0009165 nucleotide biosynthetic process | RCA GO_REF:0000123 | MARK AS OVER ANNOTATED | Summary: RCA from YeastPathways superpathway PRPP-PWY-1 (superpathway of histidine, purine, and pyrimidine biosynthesis) via the ATP:AMP reaction Adk2 does not catalyse. Not supported for Adk2. Reason: Inherited from a superpathway via the wrong (ATP:AMP) reaction. Supporting Evidence: PMID:8537371 The encoded protein exhibited GTP:AMP and ITP:AMP phosphotransferase activities but did not accept ATP as phosphate donor PMID:8537371 yeast mitochondria can completely dispense with GTP:AMP phosphotransferase activity |
| GO:0016776 phosphotransferase activity, phosphate group as acceptor | IEA GO_REF:0000120 | TOO GENERAL | Summary: IEA from ARBA00026253 / InterPro IPR006259. Correct parent of nucleoside triphosphate adenylate kinase activity, uninformative. Reason: Uninformative parent of the core MF. Supporting Evidence: file:yeast/ADK2/ADK2-uniprot.txt Reaction=a ribonucleoside 5'-triphosphate + AMP = a ribonucleoside 5'- |
| GO:0019205 nucleobase-containing compound kinase activity | IEA GO_REF:0000120 | TOO GENERAL | Summary: IEA from ARBA00028083 / InterPro IPR000850. Correct parent, uninformative. Reason: Uninformative parent of the core MF. Supporting Evidence: file:yeast/ADK2/ADK2-uniprot.txt Reaction=a ribonucleoside 5'-triphosphate + AMP = a ribonucleoside 5'- |
| GO:0043101 purine-containing compound salvage | RCA GO_REF:0000123 | MARK AS OVER ANNOTATED | Summary: RCA from YeastPathways PWY3O-285 (superpathway of purine biosynthesis and salvage pathways). Adk2 is a dispensable matrix GTP:AMP phosphotransferase; no evidence for a role in purine salvage. Reason: Inherited from a biosynthesis+salvage superpathway via the wrong reaction. Supporting Evidence: PMID:8537371 yeast mitochondria can completely dispense with GTP:AMP phosphotransferase activity |
| GO:0046033 AMP metabolic process | IBA GO_REF:0000033 | ACCEPT | Summary: IBA from PTN008598552 (AK3 clade). AMP is the phosphate acceptor of Adk2; correct and the most appropriate BP for this enzyme. Supporting Evidence: file:yeast/ADK2/ADK2-uniprot.txt Reaction=a ribonucleoside 5'-triphosphate + AMP = a ribonucleoside 5'- |
| GO:0046033 AMP metabolic process | IEA GO_REF:0000104 | ACCEPT | Summary: IEA from HAMAP MF_03169. Correct. Supporting Evidence: file:yeast/ADK2/ADK2-uniprot.txt Reaction=a ribonucleoside 5'-triphosphate + AMP = a ribonucleoside 5'- |
| GO:0046039 GTP metabolic process | IEA GO_REF:0000104 | KEEP AS NON CORE | Summary: IEA from HAMAP MF_03169 (GTP metabolic process). Adk2 uses GTP as phosphate donor (GTP:AMP and ITP:AMP activities), so it participates in GTP metabolism; correct but secondary. Reason: GTP is a phosphate donor of Adk2; correct but secondary. Supporting Evidence: PMID:8537371 The encoded protein exhibited GTP:AMP and ITP:AMP phosphotransferase activities but did not accept ATP as phosphate donor |
| GO:0046041 ITP metabolic process | IEA GO_REF:0000104 | KEEP AS NON CORE | Summary: IEA from HAMAP MF_03169 (ITP metabolic process). Adk2 uses ITP as phosphate donor (GTP:AMP and ITP:AMP activities), so it participates in ITP metabolism; correct but secondary. Reason: ITP is a phosphate donor of Adk2; correct but secondary. Supporting Evidence: PMID:8537371 The encoded protein exhibited GTP:AMP and ITP:AMP phosphotransferase activities but did not accept ATP as phosphate donor |
| GO:0046086 adenosine biosynthetic process | RCA GO_REF:0000123 | REMOVE | Summary: RCA from YeastPathways PWY-6126-1 (superpathway of adenosine nucleotides de novo biosynthesis II); the pathway name 'adenosine ribonucleotides' was mapped to the nucleoside term 'adenosine biosynthetic process'. Adk2 does not synthesise adenosine. Reason: Adenosine is a nucleoside; Adk2 neither makes nor uses it, and the ATP:AMP reaction behind the row is not an Adk2 activity. Supporting Evidence: file:yeast/ADK2/ADK2-uniprot.txt Reaction=a ribonucleoside 5'-triphosphate + AMP = a ribonucleoside 5'- PMID:8537371 The encoded protein exhibited GTP:AMP and ITP:AMP phosphotransferase activities but did not accept ATP as phosphate donor |
| GO:0046086 adenosine biosynthetic process | RCA GO_REF:0000123 | REMOVE | Summary: RCA from YeastPathways PWY-7219 (adenosine ribonucleotides de novo biosynthesis); the pathway name 'adenosine ribonucleotides' was mapped to the nucleoside term 'adenosine biosynthetic process'. Adk2 does not synthesise adenosine. Reason: Adenosine is a nucleoside; Adk2 neither makes nor uses it, and the ATP:AMP reaction behind the row is not an Adk2 activity. Supporting Evidence: file:yeast/ADK2/ADK2-uniprot.txt Reaction=a ribonucleoside 5'-triphosphate + AMP = a ribonucleoside 5'- PMID:8537371 The encoded protein exhibited GTP:AMP and ITP:AMP phosphotransferase activities but did not accept ATP as phosphate donor |
| GO:0046899 nucleoside triphosphate adenylate kinase activity | IBA GO_REF:0000033 | ACCEPT | Summary: IBA from PTN008598552 (AK3 clade, GTP:AMP phosphotransferase). Correct; core MF. Supporting Evidence: file:yeast/ADK2/ADK2-uniprot.txt Reaction=a ribonucleoside 5'-triphosphate + AMP = a ribonucleoside 5'- file:yeast/ADK2/ADK2-uniprot.txt Belongs to the adenylate kinase family. AK3 subfamily. |
| GO:0046899 nucleoside triphosphate adenylate kinase activity | IDA PMID:15753074 A GTP:AMP phosphotransferase, Adk2p, in Saccharomyces cerevi... | ACCEPT | Summary: IDA (Gu et al. 2005): purified short and long forms of Adk2 are both enzymatically active GTP:AMP phosphotransferases at 30 C. Core MF. Supporting Evidence: PMID:15753074 both short and long forms of Adk2p are enzymatically active |
| GO:0046899 nucleoside triphosphate adenylate kinase activity | IDA PMID:8537371 Strain-dependent occurrence of functional GTP:AMP phosphotra... | ACCEPT | Summary: IDA (Schricker et al. 1995): the active AKY3 allele product showed GTP:AMP and ITP:AMP phosphotransferase activity but did not use ATP. Core MF. Supporting Evidence: PMID:8537371 The encoded protein exhibited GTP:AMP and ITP:AMP phosphotransferase activities but did not accept ATP as phosphate donor |
| GO:0046899 nucleoside triphosphate adenylate kinase activity | IEA GO_REF:0000120 | ACCEPT | Summary: IEA via RHEA:13749 / EC 2.7.4.10 and HAMAP MF_03169. Correct. Supporting Evidence: file:yeast/ADK2/ADK2-uniprot.txt Reaction=a ribonucleoside 5'-triphosphate + AMP = a ribonucleoside 5'- |
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Download this section (compressed HTML)Q: What is the physiological role of the matrix GTP:AMP phosphotransferase, given that its loss has no phenotype (e.g. recycling AMP from matrix acyl-CoA synthetases using succinyl-CoA synthetase-derived GTP)?
Q: Is the inner-membrane association of Adk2 functionally significant or a fractionation artefact?
Experiment: Measure matrix AMP/GTP/GDP pools and acyl-CoA synthetase-dependent processes (e.g. lipoylation, mtFAS) in adk2 null cells under respiratory growth and heat stress.
Mitochondrial adenylate kinase; catalyzes the reversible synthesis of GTP and AMP from GDP and ADP; may serve as a back-up for synthesizing GTP or ADP depending on metabolic conditions; 3' sequence of ADK2 varies with strain background
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