| Property | Details |
|---|---|
| Gene Name | **AMD2**; synonyms reported in UniProt: **AMDY, AMDY1** |
| Systematic Name | **YDR242W** |
| UniProt Accession | **P22580** |
| Organism | **Saccharomyces cerevisiae** (strain ATCC 204508 / S288c), baker's yeast |
| EC Number | **EC 3.5.1.4** (amidase / acylamide amidohydrolase), assigned as a probable function by annotation rather than direct biochemical validation for AMD2 (pqac-00000026, pqac-00000027) |
| Protein Family | **Amidase signature (AS) family**; broadly distributed amidohydrolases characterized by a conserved serine/glycine-rich amidase-signature region (pqac-00000035, pqac-00000036, pqac-00000037) |
| Key Domains | **Amidase_CS (IPR020556)**, **Amidase_dom (IPR023631)**, **AS_sf (IPR036928)**; consistent with UniProt/InterPro annotation and the AS-family structural framework (pqac-00000034, pqac-00000035) |
| Predicted Catalytic Triad | **Ser-cisSer-Lys**; canonical catalytic configuration of amidase-signature enzymes (pqac-00000034, pqac-00000035, pqac-00000036) |
| Predicted Function | **Probable amidase** predicted to hydrolyze amide bonds, yielding a carboxylic acid plus ammonia; however, the exact physiological substrate of AMD2 is unknown (pqac-00000027, pqac-00000032) |
| Subcellular Localization | **Cytoplasm**; consistent with database annotation and with a soluble non-membrane amidase, though direct AMD2-focused localization evidence is limited in the retrieved literature |
| Protein Length | **598 amino acids** (UniProt) |
| Functional Status | **Uncharacterized / probable**; there is no direct experimental validation of AMD2 enzymatic activity or native substrate specificity in the retrieved AMD2-specific literature |
| Note on Acetamide | Wild-type **S. cerevisiae S288C does not grow on acetamide as a sole nitrogen source**, indicating AMD2 is unlikely to function as a classical acetamidase for acetamide under those conditions, despite amidase-family annotation (pqac-00000007, pqac-00000008, pqac-00000011) |


*Table: This table condenses the verified identity, predicted biochemical role, family/domain architecture, and current evidence gaps for the yeast gene AMD2/YDR242W. It is useful for distinguishing annotation-based inference from experimentally demonstrated function, especially the key point that wild-type S. cerevisiae does not utilize acetamide.*