CCT2 encodes the beta subunit of the eukaryotic cytosolic chaperonin TRiC (also called CCT), a hetero-oligomeric complex composed of eight paralogous subunits arranged in two stacked rings. TRiC assists the ATP-dependent folding of approximately 10% of cytosolic proteins, with actin and tubulin being its best-characterized obligate substrates. Cct2p, like other TRiC subunits, contributes both to the ATPase-driven conformational cycle that encapsulates substrates in the folding chamber and to substrate recognition through its apical domain. In yeast, TRiC is essential and required for proper assembly of the actin cytoskeleton and tubulin-based structures including the mitotic spindle. Orthologous to human CCT2.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
|
GO:0006457
protein folding
|
IBA
GO_REF:0000033 |
ACCEPT |
Summary: Manual review: protein folding is consistent with known biology of CCT2.
Reason: Retained as supported or plausible for this gene and evidence context.
|
|
GO:0005832
chaperonin-containing T-complex
|
IBA
GO_REF:0000033 |
ACCEPT |
Summary: Manual review: chaperonin-containing T-complex is consistent with known biology of CCT2.
Reason: Retained as supported or plausible for this gene and evidence context.
|
|
GO:0051082
unfolded protein binding
|
IBA
GO_REF:0000033 |
MODIFY |
Summary: Manual review: unfolded protein binding is better represented by a more specific replacement term for CCT2.
Reason: Modified to align with current curation guidance and improve term specificity.
Proposed replacements:
protein folding chaperone
|
|
GO:0000166
nucleotide binding
|
IEA
GO_REF:0000043 |
ACCEPT |
Summary: Manual review: nucleotide binding is consistent with known biology of CCT2.
Reason: Retained as supported or plausible for this gene and evidence context.
|
|
GO:0005524
ATP binding
|
IEA
GO_REF:0000120 |
ACCEPT |
Summary: Manual review: ATP binding is consistent with known biology of CCT2.
Reason: Retained as supported or plausible for this gene and evidence context.
|
|
GO:0005737
cytoplasm
|
IEA
GO_REF:0000044 |
ACCEPT |
Summary: Manual review: cytoplasm is consistent with known biology of CCT2.
Reason: Retained as supported or plausible for this gene and evidence context.
|
|
GO:0005829
cytosol
|
IEA
GO_REF:0000002 |
ACCEPT |
Summary: Manual review: cytosol is consistent with known biology of CCT2.
Reason: Retained as supported or plausible for this gene and evidence context.
|
|
GO:0005832
chaperonin-containing T-complex
|
IEA
GO_REF:0000120 |
ACCEPT |
Summary: Manual review: chaperonin-containing T-complex is consistent with known biology of CCT2.
Reason: Retained as supported or plausible for this gene and evidence context.
|
|
GO:0006457
protein folding
|
IEA
GO_REF:0000120 |
ACCEPT |
Summary: Manual review: protein folding is consistent with known biology of CCT2.
Reason: Retained as supported or plausible for this gene and evidence context.
|
|
GO:0016887
ATP hydrolysis activity
|
IEA
GO_REF:0000002 |
ACCEPT |
Summary: Manual review: ATP hydrolysis activity is consistent with known biology of CCT2.
Reason: Retained as supported or plausible for this gene and evidence context.
|
|
GO:0051082
unfolded protein binding
|
IEA
GO_REF:0000120 |
MODIFY |
Summary: Manual review: unfolded protein binding is better represented by a more specific replacement term for CCT2.
Reason: Modified to align with current curation guidance and improve term specificity.
Proposed replacements:
protein folding chaperone
|
|
GO:0140662
ATP-dependent protein folding chaperone
|
IEA
GO_REF:0000002 |
ACCEPT |
Summary: Manual review: ATP-dependent protein folding chaperone is consistent with known biology of CCT2.
Reason: Retained as supported or plausible for this gene and evidence context.
|
|
GO:0005515
protein binding
|
IPI
PMID:16554755 Global landscape of protein complexes in the yeast Saccharom... |
MARK AS OVER ANNOTATED |
Summary: Manual review: protein binding is too generic or over-extended for CCT2.
Reason: Marked over-annotated because more specific terms capture the biology more accurately.
|
|
GO:0005515
protein binding
|
IPI
PMID:19536198 An atlas of chaperone-protein interactions in Saccharomyces ... |
MARK AS OVER ANNOTATED |
Summary: Manual review: protein binding is too generic or over-extended for CCT2.
Reason: Marked over-annotated because more specific terms capture the biology more accurately.
|
|
GO:0005515
protein binding
|
IPI
PMID:37968396 The social and structural architecture of the yeast protein ... |
MARK AS OVER ANNOTATED |
Summary: Manual review: protein binding is too generic or over-extended for CCT2.
Reason: Marked over-annotated because more specific terms capture the biology more accurately.
|
|
GO:0006457
protein folding
|
IDA
PMID:16762366 Quantitative actin folding reactions using yeast CCT purifie... |
ACCEPT |
Summary: Manual review: protein folding is consistent with known biology of CCT2.
Reason: Retained as supported or plausible for this gene and evidence context.
|
|
GO:0005515
protein binding
|
IPI
PMID:17429077 Functional interaction between phosducin-like protein 2 and ... |
MARK AS OVER ANNOTATED |
Summary: Manual review: protein binding is too generic or over-extended for CCT2.
Reason: Marked over-annotated because more specific terms capture the biology more accurately.
|
|
GO:0005737
cytoplasm
|
HDA
PMID:11914276 Subcellular localization of the yeast proteome. |
ACCEPT |
Summary: Manual review: cytoplasm is consistent with known biology of CCT2.
Reason: Retained as supported or plausible for this gene and evidence context.
|
|
GO:0005832
chaperonin-containing T-complex
|
IPI
PMID:15704212 Physiological effects of unassembled chaperonin Cct subunits... |
ACCEPT |
Summary: Manual review: chaperonin-containing T-complex is consistent with known biology of CCT2.
Reason: Retained as supported or plausible for this gene and evidence context.
|
|
GO:0005832
chaperonin-containing T-complex
|
IDA
PMID:16762366 Quantitative actin folding reactions using yeast CCT purifie... |
ACCEPT |
Summary: Manual review: chaperonin-containing T-complex is consistent with known biology of CCT2.
Reason: Retained as supported or plausible for this gene and evidence context.
|
|
GO:0051082
unfolded protein binding
|
IDA
PMID:16762366 Quantitative actin folding reactions using yeast CCT purifie... |
MODIFY |
Summary: Manual review: unfolded protein binding is better represented by a more specific replacement term for CCT2.
Reason: Modified to align with current curation guidance and improve term specificity.
Proposed replacements:
protein folding chaperone
|
id: P39076
gene_symbol: CCT2
product_type: PROTEIN
status: DRAFT
taxon:
id: NCBITaxon:559292
label: Saccharomyces cerevisiae
description: >-
CCT2 encodes the beta subunit of the eukaryotic cytosolic chaperonin TRiC
(also called CCT), a hetero-oligomeric complex composed of eight paralogous
subunits arranged in two stacked rings. TRiC assists the ATP-dependent
folding of approximately 10% of cytosolic proteins, with actin and tubulin
being its best-characterized obligate substrates. Cct2p, like other TRiC
subunits, contributes both to the ATPase-driven conformational cycle that
encapsulates substrates in the folding chamber and to substrate recognition
through its apical domain. In yeast, TRiC is essential and required for
proper assembly of the actin cytoskeleton and tubulin-based structures
including the mitotic spindle. Orthologous to human CCT2.
existing_annotations:
- term:
id: GO:0006457
label: protein folding
evidence_type: IBA
original_reference_id: GO_REF:0000033
review:
summary: 'Manual review: protein folding is consistent with known biology of CCT2.'
action: ACCEPT
reason: Retained as supported or plausible for this gene and evidence context.
- term:
id: GO:0005832
label: chaperonin-containing T-complex
evidence_type: IBA
original_reference_id: GO_REF:0000033
review:
summary: 'Manual review: chaperonin-containing T-complex is consistent with known biology of CCT2.'
action: ACCEPT
reason: Retained as supported or plausible for this gene and evidence context.
- term:
id: GO:0051082
label: unfolded protein binding
evidence_type: IBA
original_reference_id: GO_REF:0000033
review:
summary: 'Manual review: unfolded protein binding is better represented by a more specific replacement term for CCT2.'
action: MODIFY
reason: Modified to align with current curation guidance and improve term specificity.
proposed_replacement_terms:
- id: GO:0044183
label: protein folding chaperone
- term:
id: GO:0000166
label: nucleotide binding
evidence_type: IEA
original_reference_id: GO_REF:0000043
review:
summary: 'Manual review: nucleotide binding is consistent with known biology of CCT2.'
action: ACCEPT
reason: Retained as supported or plausible for this gene and evidence context.
- term:
id: GO:0005524
label: ATP binding
evidence_type: IEA
original_reference_id: GO_REF:0000120
review:
summary: 'Manual review: ATP binding is consistent with known biology of CCT2.'
action: ACCEPT
reason: Retained as supported or plausible for this gene and evidence context.
- term:
id: GO:0005737
label: cytoplasm
evidence_type: IEA
original_reference_id: GO_REF:0000044
review:
summary: 'Manual review: cytoplasm is consistent with known biology of CCT2.'
action: ACCEPT
reason: Retained as supported or plausible for this gene and evidence context.
- term:
id: GO:0005829
label: cytosol
evidence_type: IEA
original_reference_id: GO_REF:0000002
review:
summary: 'Manual review: cytosol is consistent with known biology of CCT2.'
action: ACCEPT
reason: Retained as supported or plausible for this gene and evidence context.
- term:
id: GO:0005832
label: chaperonin-containing T-complex
evidence_type: IEA
original_reference_id: GO_REF:0000120
review:
summary: 'Manual review: chaperonin-containing T-complex is consistent with known biology of CCT2.'
action: ACCEPT
reason: Retained as supported or plausible for this gene and evidence context.
- term:
id: GO:0006457
label: protein folding
evidence_type: IEA
original_reference_id: GO_REF:0000120
review:
summary: 'Manual review: protein folding is consistent with known biology of CCT2.'
action: ACCEPT
reason: Retained as supported or plausible for this gene and evidence context.
- term:
id: GO:0016887
label: ATP hydrolysis activity
evidence_type: IEA
original_reference_id: GO_REF:0000002
review:
summary: 'Manual review: ATP hydrolysis activity is consistent with known biology of CCT2.'
action: ACCEPT
reason: Retained as supported or plausible for this gene and evidence context.
- term:
id: GO:0051082
label: unfolded protein binding
evidence_type: IEA
original_reference_id: GO_REF:0000120
review:
summary: 'Manual review: unfolded protein binding is better represented by a more specific replacement term for CCT2.'
action: MODIFY
reason: Modified to align with current curation guidance and improve term specificity.
proposed_replacement_terms:
- id: GO:0044183
label: protein folding chaperone
- term:
id: GO:0140662
label: ATP-dependent protein folding chaperone
evidence_type: IEA
original_reference_id: GO_REF:0000002
review:
summary: 'Manual review: ATP-dependent protein folding chaperone is consistent with known biology of CCT2.'
action: ACCEPT
reason: Retained as supported or plausible for this gene and evidence context.
- term:
id: GO:0005515
label: protein binding
evidence_type: IPI
original_reference_id: PMID:16554755
review:
summary: 'Manual review: protein binding is too generic or over-extended for CCT2.'
action: MARK_AS_OVER_ANNOTATED
reason: Marked over-annotated because more specific terms capture the biology more accurately.
- term:
id: GO:0005515
label: protein binding
evidence_type: IPI
original_reference_id: PMID:19536198
review:
summary: 'Manual review: protein binding is too generic or over-extended for CCT2.'
action: MARK_AS_OVER_ANNOTATED
reason: Marked over-annotated because more specific terms capture the biology more accurately.
- term:
id: GO:0005515
label: protein binding
evidence_type: IPI
original_reference_id: PMID:37968396
review:
summary: 'Manual review: protein binding is too generic or over-extended for CCT2.'
action: MARK_AS_OVER_ANNOTATED
reason: Marked over-annotated because more specific terms capture the biology more accurately.
- term:
id: GO:0006457
label: protein folding
evidence_type: IDA
original_reference_id: PMID:16762366
review:
summary: 'Manual review: protein folding is consistent with known biology of CCT2.'
action: ACCEPT
reason: Retained as supported or plausible for this gene and evidence context.
- term:
id: GO:0005515
label: protein binding
evidence_type: IPI
original_reference_id: PMID:17429077
review:
summary: 'Manual review: protein binding is too generic or over-extended for CCT2.'
action: MARK_AS_OVER_ANNOTATED
reason: Marked over-annotated because more specific terms capture the biology more accurately.
- term:
id: GO:0005737
label: cytoplasm
evidence_type: HDA
original_reference_id: PMID:11914276
review:
summary: 'Manual review: cytoplasm is consistent with known biology of CCT2.'
action: ACCEPT
reason: Retained as supported or plausible for this gene and evidence context.
- term:
id: GO:0005832
label: chaperonin-containing T-complex
evidence_type: IPI
original_reference_id: PMID:15704212
review:
summary: 'Manual review: chaperonin-containing T-complex is consistent with known biology of CCT2.'
action: ACCEPT
reason: Retained as supported or plausible for this gene and evidence context.
- term:
id: GO:0005832
label: chaperonin-containing T-complex
evidence_type: IDA
original_reference_id: PMID:16762366
review:
summary: 'Manual review: chaperonin-containing T-complex is consistent with known biology of CCT2.'
action: ACCEPT
reason: Retained as supported or plausible for this gene and evidence context.
- term:
id: GO:0051082
label: unfolded protein binding
evidence_type: IDA
original_reference_id: PMID:16762366
review:
summary: 'Manual review: unfolded protein binding is better represented by a more specific replacement term for CCT2.'
action: MODIFY
reason: Modified to align with current curation guidance and improve term specificity.
proposed_replacement_terms:
- id: GO:0044183
label: protein folding chaperone
references:
- id: GO_REF:0000002
title: Gene Ontology annotation through association of InterPro records with GO terms
findings: []
- id: GO_REF:0000033
title: Annotation inferences using phylogenetic trees
findings: []
- id: GO_REF:0000043
title: Gene Ontology annotation based on UniProtKB/Swiss-Prot keyword mapping
findings: []
- id: GO_REF:0000044
title: Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
findings: []
- id: GO_REF:0000120
title: Combined Automated Annotation using Multiple IEA Methods
findings: []
- id: PMID:11914276
title: Subcellular localization of the yeast proteome.
findings: []
- id: PMID:15704212
title: Physiological effects of unassembled chaperonin Cct subunits in the yeast Saccharomyces cerevisiae.
findings: []
- id: PMID:16554755
title: Global landscape of protein complexes in the yeast Saccharomyces cerevisiae.
findings: []
- id: PMID:16762366
title: Quantitative actin folding reactions using yeast CCT purified via an internal tag in the CCT3/gamma subunit.
findings: []
- id: PMID:17429077
title: Functional interaction between phosducin-like protein 2 and cytosolic chaperonin is essential for cytoskeletal protein function and cell cycle progression.
findings: []
- id: PMID:19536198
title: 'An atlas of chaperone-protein interactions in Saccharomyces cerevisiae: implications to protein folding pathways in the cell.'
findings: []
- id: PMID:37968396
title: The social and structural architecture of the yeast protein interactome.
findings: []