Histidinol-phosphate aminotransferase (EC 2.6.1.9) of Saccharomyces cerevisiae, the HisC orthologue and a class II (fold type I) pyridoxal 5'-phosphate-dependent aminotransferase. It catalyses step 7 of L-histidine biosynthesis, the reversible transamination of imidazole-acetol phosphate with L-glutamate to give L-histidinol phosphate and 2-oxoglutarate. It is a cytosolic enzyme and its gene is under GCN4 general amino acid control. Bacterial HisC enzymes can transaminate aromatic amino acids in vitro, but in yeast the aromatic aminotransferases Aro8 and Aro9 account for phenylalanine and tyrosine transamination, since aro8 aro9 double mutants are Phe/Tyr auxotrophs despite an intact HIS5.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0000105 L-histidine biosynthetic process | IEA GO_REF:0000120 | ACCEPT | Summary: Electronic annotation (InterPro/UniPathway/ARBA) consistent with the enzyme's catalytic step in histidine biosynthesis. HIS5 catalyses step 7, histidinol-phosphate aminotransferase of the pathway, so this is the core biological process. Supporting Evidence: PMID:14190241 Each of the histidine mutants of Saccharomyces cerevisiae has been correlated with a particular step in histidine biosynthesis |
| GO:0000105 L-histidine biosynthetic process | IMP PMID:14190241 GENE-ENZYME RELATIONS IN HISTIDINE BIOSYNTHESIS IN YEAST. | ACCEPT | Summary: Mutant phenotype (histidine auxotrophy with loss of the corresponding enzymatic step) directly ties the gene product to histidine biosynthesis. HIS5 catalyses step 7, histidinol-phosphate aminotransferase of the pathway, so this is the core biological process. Supporting Evidence: PMID:14190241 Each of the histidine mutants of Saccharomyces cerevisiae has been correlated with a particular step in histidine biosynthesis |
| GO:0000105 L-histidine biosynthetic process | RCA GO_REF:0000123 | ACCEPT | Summary: Derived from YeastPathways HISTSYN-PWY (L-histidine biosynthesis), in which this gene catalyses a defined step. HIS5 catalyses step 7, histidinol-phosphate aminotransferase of the pathway, so this is the core biological process. Supporting Evidence: PMID:14190241 Each of the histidine mutants of Saccharomyces cerevisiae has been correlated with a particular step in histidine biosynthesis |
| GO:0004400 L-histidinol-phosphate:2-oxoglutarate transaminase activity | IEA GO_REF:0000120 | ACCEPT | Summary: Electronic annotation from InterPro/Rhea/EC mapping; consistent with domain architecture and experimental data. This is the core catalytic activity of HIS5 (step 7, histidinol-phosphate aminotransferase). Supporting Evidence: PMID:14190241 Each of the histidine mutants of Saccharomyces cerevisiae has been correlated with a particular step in histidine biosynthesis file:yeast/HIS5/HIS5-uniprot.txt RecName: Full=Histidinol-phosphate aminotransferase {ECO:0000305}; |
| GO:0004400 L-histidinol-phosphate:2-oxoglutarate transaminase activity | IMP PMID:14190241 GENE-ENZYME RELATIONS IN HISTIDINE BIOSYNTHESIS IN YEAST. | ACCEPT | Summary: Mutants in this gene lack the corresponding enzymatic step (Fink 1964 gene-enzyme correlation). This is the core catalytic activity of HIS5 (step 7, histidinol-phosphate aminotransferase). Supporting Evidence: PMID:14190241 Each of the histidine mutants of Saccharomyces cerevisiae has been correlated with a particular step in histidine biosynthesis file:yeast/HIS5/HIS5-uniprot.txt RecName: Full=Histidinol-phosphate aminotransferase {ECO:0000305}; |
| GO:0004400 L-histidinol-phosphate:2-oxoglutarate transaminase activity | RCA GO_REF:0000123 | ACCEPT | Summary: YeastPathways (ALL-CHORISMATE-PWY-1) assignment of this reaction to HIS5; MF correct irrespective of which pathway diagram generated the row. This is the core catalytic activity of HIS5 (step 7, histidinol-phosphate aminotransferase). Supporting Evidence: PMID:14190241 Each of the histidine mutants of Saccharomyces cerevisiae has been correlated with a particular step in histidine biosynthesis file:yeast/HIS5/HIS5-uniprot.txt RecName: Full=Histidinol-phosphate aminotransferase {ECO:0000305}; |
| GO:0004400 L-histidinol-phosphate:2-oxoglutarate transaminase activity | RCA GO_REF:0000123 | ACCEPT | Summary: YeastPathways (HISTSYN-PWY) assignment of this reaction to HIS5; MF correct irrespective of which pathway diagram generated the row. This is the core catalytic activity of HIS5 (step 7, histidinol-phosphate aminotransferase). Supporting Evidence: PMID:14190241 Each of the histidine mutants of Saccharomyces cerevisiae has been correlated with a particular step in histidine biosynthesis file:yeast/HIS5/HIS5-uniprot.txt RecName: Full=Histidinol-phosphate aminotransferase {ECO:0000305}; |
| GO:0004400 L-histidinol-phosphate:2-oxoglutarate transaminase activity | RCA GO_REF:0000123 | ACCEPT | Summary: YeastPathways (PRPP-PWY-1) assignment of this reaction to HIS5; MF correct irrespective of which pathway diagram generated the row. This is the core catalytic activity of HIS5 (step 7, histidinol-phosphate aminotransferase). Supporting Evidence: PMID:14190241 Each of the histidine mutants of Saccharomyces cerevisiae has been correlated with a particular step in histidine biosynthesis file:yeast/HIS5/HIS5-uniprot.txt RecName: Full=Histidinol-phosphate aminotransferase {ECO:0000305}; |
| GO:0005737 cytoplasm | HDA PMID:14562095 Global analysis of protein localization in budding yeast. | ACCEPT | Summary: High-throughput GFP-fusion localisation (Huh et al. 2003) placed the protein in the cytoplasm. Consistent with a soluble cytosolic biosynthetic enzyme. Supporting Evidence: PMID:14562095 We classify these proteins, representing 75% of the yeast proteome, into 22 distinct subcellular localization categories |
| GO:0005829 cytosol | RCA GO_REF:0000123 | ACCEPT | Summary: RCA from YeastPathways (ALL-CHORISMATE-PWY-1). The histidine biosynthetic enzymes of S. cerevisiae are soluble cytosolic proteins with no targeting signals, so is_active_in cytosol is correct regardless of which pathway diagram generated the row. Supporting Evidence: PMID:14562095 We classify these proteins, representing 75% of the yeast proteome, into 22 distinct subcellular localization categories |
| GO:0005829 cytosol | RCA GO_REF:0000123 | ACCEPT | Summary: RCA from YeastPathways (HISTSYN-PWY). The histidine biosynthetic enzymes of S. cerevisiae are soluble cytosolic proteins with no targeting signals, so is_active_in cytosol is correct regardless of which pathway diagram generated the row. Supporting Evidence: PMID:14562095 We classify these proteins, representing 75% of the yeast proteome, into 22 distinct subcellular localization categories |
| GO:0005829 cytosol | RCA GO_REF:0000123 | ACCEPT | Summary: RCA from YeastPathways (PRPP-PWY-1). The histidine biosynthetic enzymes of S. cerevisiae are soluble cytosolic proteins with no targeting signals, so is_active_in cytosol is correct regardless of which pathway diagram generated the row. Supporting Evidence: PMID:14562095 We classify these proteins, representing 75% of the yeast proteome, into 22 distinct subcellular localization categories |
| GO:0005829 cytosol | RCA GO_REF:0000123 | ACCEPT | Summary: RCA from YeastPathways (PWY3O-4115). The histidine biosynthetic enzymes of S. cerevisiae are soluble cytosolic proteins with no targeting signals, so is_active_in cytosol is correct regardless of which pathway diagram generated the row. Supporting Evidence: PMID:14562095 We classify these proteins, representing 75% of the yeast proteome, into 22 distinct subcellular localization categories |
| GO:0006559 L-phenylalanine catabolic process | RCA GO_REF:0000123 | REMOVE | Summary: RCA from YeastPathways PWY3O-4115. Phenylalanine catabolism (Ehrlich pathway) transamination in yeast is attributed to Aro8/Aro9; there is no experimental evidence that His5 contributes, and its demonstrated role is histidine biosynthesis. The pathway-database inclusion appears to be by analogy to bacterial HisC promiscuity and is not supported for the yeast enzyme. Supporting Evidence: PMID:9491082 aro8 and aro9 double mutants which are auxotrophic for both phenylalanine and tyrosine PMID:14190241 Each of the histidine mutants of Saccharomyces cerevisiae has been correlated with a particular step in histidine biosynthesis |
| GO:0008793 aromatic-amino-acid:2-oxoglutarate transaminase activity | RCA GO_REF:0000123 | MARK AS OVER ANNOTATED | Summary: RCA from YeastPathways PWY3O-4115 (phenylalanine degradation/Ehrlich pathway transamination), which lists HIS5 alongside the aromatic aminotransferases Aro8/Aro9, presumably by analogy to the substrate promiscuity of bacterial HisC. No yeast experiment shows His5 transaminating aromatic amino acids, and aro8 aro9 double mutants are phenylalanine/tyrosine auxotrophs, indicating His5 cannot substitute in vivo. A minor in vitro side activity cannot be excluded, so marked as over-annotation rather than removed. Supporting Evidence: PMID:9491082 aro8 and aro9 double mutants which are auxotrophic for both phenylalanine and tyrosine |
| GO:0009165 nucleotide biosynthetic process | RCA GO_REF:0000123 | REMOVE | Summary: RCA propagated from the YeastPathways superpathway PRPP-PWY-1 (superpathway of histidine, purine and pyrimidine biosynthesis), which assigns the umbrella process to every member enzyme. HIS5 catalyses step 7, histidinol-phosphate aminotransferase of L-histidine biosynthesis; its substrates and products are histidine-pathway intermediates, and it performs no step of nucleotide biosynthesis. Sharing PRPP as a precursor with nucleotide synthesis is not participation. The pathway-bundling artefact should be removed. Supporting Evidence: PMID:14190241 Each of the histidine mutants of Saccharomyces cerevisiae has been correlated with a particular step in histidine biosynthesis |
| GO:0016740 transferase activity | IEA GO_REF:0000002 | MODIFY | Summary: Generic electronic annotation (transferase activity); the specific activity is L-histidinol-phosphate:2-oxoglutarate transaminase (GO:0004400). Note that histidinol phosphate is not an amino acid, so the amino-acid-transaminase grouping is also imprecise. Proposed replacements: L-histidinol-phosphate:2-oxoglutarate transaminase activity Supporting Evidence: file:yeast/HIS5/HIS5-uniprot.txt RecName: Full=Histidinol-phosphate aminotransferase {ECO:0000305}; |
| GO:0030170 pyridoxal phosphate binding | IEA GO_REF:0000002 | KEEP AS NON CORE | Summary: PLP is the cofactor of this class II aminotransferase; correct cofactor-binding annotation but non-core. Supporting Evidence: file:yeast/HIS5/HIS5-uniprot.txt Name=pyridoxal 5'-phosphate; Xref=ChEBI:CHEBI:597326; |
| GO:0046417 chorismate metabolic process | RCA GO_REF:0000123 | REMOVE | Summary: RCA from the YeastPathways superpathway ALL-CHORISMATE-PWY-1 (superpathway of chorismate metabolism), which bundles aromatic amino acid branches. His5 neither produces nor consumes chorismate, and its inclusion rests on a putative aromatic aminotransferase side activity not supported in yeast. Supporting Evidence: PMID:9491082 aro8 and aro9 double mutants which are auxotrophic for both phenylalanine and tyrosine |
| GO:0140385 amino acid transaminase activity | IEA GO_REF:0000117 | MODIFY | Summary: Generic electronic annotation (amino acid transaminase activity); the specific activity is L-histidinol-phosphate:2-oxoglutarate transaminase (GO:0004400). Note that histidinol phosphate is not an amino acid, so the amino-acid-transaminase grouping is also imprecise. Proposed replacements: L-histidinol-phosphate:2-oxoglutarate transaminase activity Supporting Evidence: file:yeast/HIS5/HIS5-uniprot.txt RecName: Full=Histidinol-phosphate aminotransferase {ECO:0000305}; |
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