Mitochondrial C1-tetrahydrofolate synthase of Saccharomyces cerevisiae, a homodimeric trifunctional enzyme (subunit about 100 kDa) and the mitochondrial isozyme of cytosolic Ade3. Its N-terminal dehydrogenase/cyclohydrolase domain carries NADP+-dependent 5,10-methylene-THF dehydrogenase (EC 1.5.1.5) and 5,10-methenyl-THF cyclohydrolase (EC 3.5.4.9) activities; its C-terminal domain is an ATP-dependent 10-formyl-THF synthetase (EC 6.3.4.3). Together they interconvert formate, 10-formyl-THF, 5,10-methenyl-THF and 5,10-methylene-THF in the mitochondrial matrix. This links mitochondrial one-carbon units, e.g. from glycine cleavage, with the cytosolic folate pool through formate.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0003824 catalytic activity | IEA GO_REF:0000002 | MARK AS OVER ANNOTATED | Summary: InterPro2GO generic catalytic activity (IPR020867); uninformative given the three specific activities. Reason: Uninformative root-level MF. |
| GO:0004329 formate-tetrahydrofolate ligase activity | IDA PMID:3528153 Purification and characterization of a mitochondrial isozyme... | ACCEPT | Summary: Shannon & Rabinowitz purified the mitochondrial isozyme from an ade3 deletion strain and showed 10-formyl-THF synthetase activity co-purifying with the other two activities. Supporting Evidence: PMID:3528153 Synthetase, cyclohydrolase, and dehydrogenase activities in an ade3 deletion strain co-purify 4,000-fold to yield a single protein species PMID:3528153 catalyzes 10-formyltetrahydrofolate synthetase (EC 6.3.4.3), 5,10-methenyltetrahydrofolate cyclohydrolase (EC 3.5.4.9), and 5,10-methylenetetrahydrofolate dehydrogenase (EC 1.5.1.5) activities |
| GO:0004329 formate-tetrahydrofolate ligase activity | IEA GO_REF:0000120 | ACCEPT | Summary: Combined IEA (InterPro FTHFS IPR000559, RHEA:20221, EC 6.3.4.3); correct for the C-terminal synthetase domain. Supporting Evidence: file:yeast/MIS1/MIS1-uniprot.txt (6S)-5,6,7,8-tetrahydrofolate + formate + ATP = (6R)-10- |
| GO:0004329 formate-tetrahydrofolate ligase activity | RCA GO_REF:0000123 | ACCEPT | Summary: YeastPathways RCA from ALL-CHORISMATE-PWY-1 (superpathway of chorismate metabolism) assigning Mis1 to the formate-tetrahydrofolate ligase reaction. The activity is experimentally shown for the mitochondrial isozyme; correct whatever the pathway context. Supporting Evidence: PMID:3528153 catalyzes 10-formyltetrahydrofolate synthetase (EC 6.3.4.3), 5,10-methenyltetrahydrofolate cyclohydrolase (EC 3.5.4.9), and 5,10-methylenetetrahydrofolate dehydrogenase (EC 1.5.1.5) activities |
| GO:0004329 formate-tetrahydrofolate ligase activity | RCA GO_REF:0000123 | ACCEPT | Summary: YeastPathways RCA from FOLSYN-PWY-1 (folate transformations) assigning Mis1 to the formate-tetrahydrofolate ligase reaction. The activity is experimentally shown for the mitochondrial isozyme; correct whatever the pathway context. Supporting Evidence: PMID:3528153 catalyzes 10-formyltetrahydrofolate synthetase (EC 6.3.4.3), 5,10-methenyltetrahydrofolate cyclohydrolase (EC 3.5.4.9), and 5,10-methylenetetrahydrofolate dehydrogenase (EC 1.5.1.5) activities |
| GO:0004329 formate-tetrahydrofolate ligase activity | RCA GO_REF:0000123 | ACCEPT | Summary: YeastPathways RCA from PWY-2201 (folate transformations I) assigning Mis1 to the formate-tetrahydrofolate ligase reaction. The activity is experimentally shown for the mitochondrial isozyme; correct whatever the pathway context. Supporting Evidence: PMID:3528153 catalyzes 10-formyltetrahydrofolate synthetase (EC 6.3.4.3), 5,10-methenyltetrahydrofolate cyclohydrolase (EC 3.5.4.9), and 5,10-methylenetetrahydrofolate dehydrogenase (EC 1.5.1.5) activities |
| GO:0004329 formate-tetrahydrofolate ligase activity | RCA GO_REF:0000123 | ACCEPT | Summary: YeastPathways RCA from PWY3O-20 (folate polyglutamylation) assigning Mis1 to the formate-tetrahydrofolate ligase reaction. The activity is experimentally shown for the mitochondrial isozyme; correct whatever the pathway context. Supporting Evidence: PMID:3528153 catalyzes 10-formyltetrahydrofolate synthetase (EC 6.3.4.3), 5,10-methenyltetrahydrofolate cyclohydrolase (EC 3.5.4.9), and 5,10-methylenetetrahydrofolate dehydrogenase (EC 1.5.1.5) activities |
| GO:0004329 formate-tetrahydrofolate ligase activity | RCA GO_REF:0000123 | ACCEPT | Summary: YeastPathways RCA from PWY3O-697 (folate interconversions) assigning Mis1 to the formate-tetrahydrofolate ligase reaction. The activity is experimentally shown for the mitochondrial isozyme; correct whatever the pathway context. Supporting Evidence: PMID:3528153 catalyzes 10-formyltetrahydrofolate synthetase (EC 6.3.4.3), 5,10-methenyltetrahydrofolate cyclohydrolase (EC 3.5.4.9), and 5,10-methylenetetrahydrofolate dehydrogenase (EC 1.5.1.5) activities |
| GO:0004477 methenyltetrahydrofolate cyclohydrolase activity | IBA GO_REF:0000033 | ACCEPT | Summary: PAINT IBA (PTN000002250) for methenyl-THF cyclohydrolase in the DHFR/CH family; Mis1 has direct evidence. Supporting Evidence: PMID:3528153 catalyzes 10-formyltetrahydrofolate synthetase (EC 6.3.4.3), 5,10-methenyltetrahydrofolate cyclohydrolase (EC 3.5.4.9), and 5,10-methylenetetrahydrofolate dehydrogenase (EC 1.5.1.5) activities |
| GO:0004477 methenyltetrahydrofolate cyclohydrolase activity | IDA PMID:3528153 Purification and characterization of a mitochondrial isozyme... | ACCEPT | Summary: Cyclohydrolase activity co-purifies with the mitochondrial isozyme. Supporting Evidence: PMID:3528153 Synthetase, cyclohydrolase, and dehydrogenase activities in an ade3 deletion strain co-purify 4,000-fold to yield a single protein species |
| GO:0004477 methenyltetrahydrofolate cyclohydrolase activity | IEA GO_REF:0000120 | ACCEPT | Summary: Combined IEA (ARBA, RHEA:23700, EC 3.5.4.9); correct. Supporting Evidence: PMID:3528153 catalyzes 10-formyltetrahydrofolate synthetase (EC 6.3.4.3), 5,10-methenyltetrahydrofolate cyclohydrolase (EC 3.5.4.9), and 5,10-methylenetetrahydrofolate dehydrogenase (EC 1.5.1.5) activities |
| GO:0004477 methenyltetrahydrofolate cyclohydrolase activity | RCA GO_REF:0000123 | ACCEPT | Summary: YeastPathways RCA from ALL-CHORISMATE-PWY-1 (superpathway of chorismate metabolism) assigning Mis1 to the methenyltetrahydrofolate cyclohydrolase reaction. The activity is experimentally shown for the mitochondrial isozyme; correct whatever the pathway context. Supporting Evidence: PMID:3528153 catalyzes 10-formyltetrahydrofolate synthetase (EC 6.3.4.3), 5,10-methenyltetrahydrofolate cyclohydrolase (EC 3.5.4.9), and 5,10-methylenetetrahydrofolate dehydrogenase (EC 1.5.1.5) activities |
| GO:0004477 methenyltetrahydrofolate cyclohydrolase activity | RCA GO_REF:0000123 | ACCEPT | Summary: YeastPathways RCA from FOLSYN-PWY-1 (folate transformations) assigning Mis1 to the methenyltetrahydrofolate cyclohydrolase reaction. The activity is experimentally shown for the mitochondrial isozyme; correct whatever the pathway context. Supporting Evidence: PMID:3528153 catalyzes 10-formyltetrahydrofolate synthetase (EC 6.3.4.3), 5,10-methenyltetrahydrofolate cyclohydrolase (EC 3.5.4.9), and 5,10-methylenetetrahydrofolate dehydrogenase (EC 1.5.1.5) activities |
| GO:0004477 methenyltetrahydrofolate cyclohydrolase activity | RCA GO_REF:0000123 | ACCEPT | Summary: YeastPathways RCA from PWY-2201 (folate transformations I) assigning Mis1 to the methenyltetrahydrofolate cyclohydrolase reaction. The activity is experimentally shown for the mitochondrial isozyme; correct whatever the pathway context. Supporting Evidence: PMID:3528153 catalyzes 10-formyltetrahydrofolate synthetase (EC 6.3.4.3), 5,10-methenyltetrahydrofolate cyclohydrolase (EC 3.5.4.9), and 5,10-methylenetetrahydrofolate dehydrogenase (EC 1.5.1.5) activities |
| GO:0004477 methenyltetrahydrofolate cyclohydrolase activity | RCA GO_REF:0000123 | ACCEPT | Summary: YeastPathways RCA from PWY3O-697 (folate interconversions) assigning Mis1 to the methenyltetrahydrofolate cyclohydrolase reaction. The activity is experimentally shown for the mitochondrial isozyme; correct whatever the pathway context. Supporting Evidence: PMID:3528153 catalyzes 10-formyltetrahydrofolate synthetase (EC 6.3.4.3), 5,10-methenyltetrahydrofolate cyclohydrolase (EC 3.5.4.9), and 5,10-methylenetetrahydrofolate dehydrogenase (EC 1.5.1.5) activities |
| GO:0004488 methylenetetrahydrofolate dehydrogenase (NADP+) activity | IBA GO_REF:0000033 | ACCEPT | Summary: PAINT IBA for NADP+-dependent methylene-THF dehydrogenase; Mis1 has direct evidence. Supporting Evidence: PMID:3528153 catalyzes 10-formyltetrahydrofolate synthetase (EC 6.3.4.3), 5,10-methenyltetrahydrofolate cyclohydrolase (EC 3.5.4.9), and 5,10-methylenetetrahydrofolate dehydrogenase (EC 1.5.1.5) activities |
| GO:0004488 methylenetetrahydrofolate dehydrogenase (NADP+) activity | IDA PMID:3528153 Purification and characterization of a mitochondrial isozyme... | ACCEPT | Summary: Dehydrogenase activity co-purifies with the mitochondrial isozyme. Supporting Evidence: PMID:3528153 Synthetase, cyclohydrolase, and dehydrogenase activities in an ade3 deletion strain co-purify 4,000-fold to yield a single protein species |
| GO:0004488 methylenetetrahydrofolate dehydrogenase (NADP+) activity | IEA GO_REF:0000120 | ACCEPT | Summary: Combined IEA (ARBA, InterPro THF DH/CH domains, RHEA:22812, EC 1.5.1.5); correct. Supporting Evidence: PMID:3528153 catalyzes 10-formyltetrahydrofolate synthetase (EC 6.3.4.3), 5,10-methenyltetrahydrofolate cyclohydrolase (EC 3.5.4.9), and 5,10-methylenetetrahydrofolate dehydrogenase (EC 1.5.1.5) activities |
| GO:0004488 methylenetetrahydrofolate dehydrogenase (NADP+) activity | RCA GO_REF:0000123 | ACCEPT | Summary: YeastPathways RCA from PWY3O-697 (folate interconversions) assigning Mis1 to the NADP+-dependent methylenetetrahydrofolate dehydrogenase reaction. The activity is experimentally shown for the mitochondrial isozyme; correct whatever the pathway context. Supporting Evidence: PMID:3528153 catalyzes 10-formyltetrahydrofolate synthetase (EC 6.3.4.3), 5,10-methenyltetrahydrofolate cyclohydrolase (EC 3.5.4.9), and 5,10-methylenetetrahydrofolate dehydrogenase (EC 1.5.1.5) activities |
| GO:0005524 ATP binding | IEA GO_REF:0000002 | ACCEPT | Summary: InterPro2GO ATP binding (FTHFS domain); the synthetase reaction consumes ATP. Supporting Evidence: file:yeast/MIS1/MIS1-uniprot.txt (6S)-5,6,7,8-tetrahydrofolate + formate + ATP = (6R)-10- |
| GO:0005737 cytoplasm | RCA GO_REF:0000123 | REMOVE | Summary: YeastPathways RCA (PWY3O-697) placing Mis1 activity in the cytoplasm. Mis1 is the mitochondrial C1-THF synthase; cell fractionation shows the isozyme, unlike cytosolic Ade3, in mitochondria, and proteomics and GFP agree. The cytoplasmic folate-interconversion reactions are carried out by Ade3 (and Mtd1). Reason: Compartment taken from the pathway, which mixes the cytosolic Ade3 and mitochondrial Mis1 isozymes. Supporting Evidence: PMID:3528153 Cell fractionation experiments show that the isozyme, but not C1-tetrahydrofolate synthase, is localized in the mitochondria |
| GO:0005739 mitochondrion | HDA PMID:14576278 The proteome of Saccharomyces cerevisiae mitochondria. | ACCEPT | Summary: Mis1 found in the purified mitochondrial proteome. Supporting Evidence: PMID:14576278 The proteins of highly pure yeast mitochondria were separated by several independent methods and analyzed by tandem MS |
| GO:0005739 mitochondrion | HDA PMID:16823961 Toward the complete yeast mitochondrial proteome: multidimen... | ACCEPT | Summary: Mis1 found in the PROMITO mitochondrial proteome. Supporting Evidence: PMID:16823961 A total of 851 different proteins (PROMITO dataset) were |
| GO:0005739 mitochondrion | HDA PMID:24769239 Quantitative variations of the mitochondrial proteome and ph... | ACCEPT | Summary: Mis1 quantified in the mitochondrial proteome. Supporting Evidence: PMID:24769239 The yeast Saccharomyces cerevisiae is a facultative aerobe able to adapt its metabolism according to the carbon substrate |
| GO:0005739 mitochondrion | IBA GO_REF:0000033 | ACCEPT | Summary: PAINT IBA at the mitochondrial isozyme node (PTN000002392), with Mis1 as the experimental leaf. Supporting Evidence: PMID:3528153 Cell fractionation experiments show that the isozyme, but not C1-tetrahydrofolate synthase, is localized in the mitochondria |
| GO:0005739 mitochondrion | IDA PMID:3528153 Purification and characterization of a mitochondrial isozyme... | ACCEPT | Summary: Cell fractionation localises the isozyme to mitochondria. Supporting Evidence: PMID:3528153 Cell fractionation experiments show that the isozyme, but not C1-tetrahydrofolate synthase, is localized in the mitochondria |
| GO:0005739 mitochondrion | IEA GO_REF:0000044 | ACCEPT | Summary: UniProt SubCell mapping (mitochondrion); correct. Supporting Evidence: PMID:3528153 Cell fractionation experiments show that the isozyme, but not C1-tetrahydrofolate synthase, is localized in the mitochondria file:yeast/MIS1/MIS1-uniprot.txt Mitochondrial isozyme of C-1-tetrahydrofolate synthase. The |
| GO:0005829 cytosol | IBA GO_REF:0000033 | REMOVE | Summary: PAINT IBA for cytosol at the broad C1-THF synthase node (PTN000002250), which includes cytosolic Ade3 and Mtd1. Mis1 is the mitochondrial C1-THF synthase; cell fractionation shows the isozyme, unlike cytosolic Ade3, in mitochondria, and proteomics and GFP agree. Mis1 has diverged into a mitochondrial paralog, so the cytosolic location does not propagate to it. Reason: Target-specific evidence that Mis1 is mitochondrial; the IBA node conflates cytosolic and mitochondrial paralogs. Propagation Review Root cause: PROPAGATION BAD Failure modes: COMPARTMENT OR COMPLEX MISMATCH WRONG ORTHOLOG OR PARALOG Sources checked: PANTHER:PTN000002250 · PTN000002250 SUPPORTS SOURCE BUT NOT TARGET Cytosol is correct for Ade3/Mtd1/MTHFD1 leaves; Mis1 is the experimentally mitochondrial isozyme and separately receives mitochondrion from PTN000002392. Supporting Evidence: PMID:3528153 Cell fractionation experiments show that the isozyme, but not C1-tetrahydrofolate synthase, is localized in the mitochondria |
| GO:0005829 cytosol | RCA GO_REF:0000123 | REMOVE | Summary: YeastPathways RCA from ALL-CHORISMATE-PWY-1 (superpathway of chorismate metabolism) placing Mis1 activity in the cytosol (default compartment). Mis1 is the mitochondrial C1-THF synthase; cell fractionation shows the isozyme, unlike cytosolic Ade3, in mitochondria, and proteomics and GFP agree. Reason: Default-compartment artefact; Mis1 is mitochondrial. Supporting Evidence: PMID:3528153 Cell fractionation experiments show that the isozyme, but not C1-tetrahydrofolate synthase, is localized in the mitochondria |
| GO:0005829 cytosol | RCA GO_REF:0000123 | REMOVE | Summary: YeastPathways RCA from FOLSYN-PWY-1 (folate transformations) placing Mis1 activity in the cytosol (default compartment). Mis1 is the mitochondrial C1-THF synthase; cell fractionation shows the isozyme, unlike cytosolic Ade3, in mitochondria, and proteomics and GFP agree. Reason: Default-compartment artefact; Mis1 is mitochondrial. Supporting Evidence: PMID:3528153 Cell fractionation experiments show that the isozyme, but not C1-tetrahydrofolate synthase, is localized in the mitochondria |
| GO:0005829 cytosol | RCA GO_REF:0000123 | REMOVE | Summary: YeastPathways RCA from PWY-2201 (folate transformations I) placing Mis1 activity in the cytosol (default compartment). Mis1 is the mitochondrial C1-THF synthase; cell fractionation shows the isozyme, unlike cytosolic Ade3, in mitochondria, and proteomics and GFP agree. Reason: Default-compartment artefact; Mis1 is mitochondrial. Supporting Evidence: PMID:3528153 Cell fractionation experiments show that the isozyme, but not C1-tetrahydrofolate synthase, is localized in the mitochondria |
| GO:0005829 cytosol | RCA GO_REF:0000123 | REMOVE | Summary: YeastPathways RCA from PWY3O-20 (folate polyglutamylation) placing Mis1 activity in the cytosol (default compartment). Mis1 is the mitochondrial C1-THF synthase; cell fractionation shows the isozyme, unlike cytosolic Ade3, in mitochondria, and proteomics and GFP agree. Reason: Default-compartment artefact; Mis1 is mitochondrial. Supporting Evidence: PMID:3528153 Cell fractionation experiments show that the isozyme, but not C1-tetrahydrofolate synthase, is localized in the mitochondria |
| GO:0005829 cytosol | RCA GO_REF:0000123 | REMOVE | Summary: YeastPathways RCA from PWY3O-697 (folate interconversions) placing Mis1 activity in the cytosol (default compartment). Mis1 is the mitochondrial C1-THF synthase; cell fractionation shows the isozyme, unlike cytosolic Ade3, in mitochondria, and proteomics and GFP agree. Reason: Default-compartment artefact; Mis1 is mitochondrial. Supporting Evidence: PMID:3528153 Cell fractionation experiments show that the isozyme, but not C1-tetrahydrofolate synthase, is localized in the mitochondria |
| GO:0016874 ligase activity | IEA GO_REF:0000117 | MARK AS OVER ANNOTATED | Summary: ARBA ligase activity; uninformative parent of formate-tetrahydrofolate ligase activity. Reason: Uninformative parent of GO:0004329. |
| GO:0035999 folate cycle | IBA GO_REF:0000033 | ACCEPT | Summary: PAINT IBA for tetrahydrofolate interconversion; all three Mis1 activities are THF one-carbon interconversions. Supporting Evidence: PMID:3528153 catalyzes 10-formyltetrahydrofolate synthetase (EC 6.3.4.3), 5,10-methenyltetrahydrofolate cyclohydrolase (EC 3.5.4.9), and 5,10-methylenetetrahydrofolate dehydrogenase (EC 1.5.1.5) activities |
| GO:0035999 folate cycle | IEA GO_REF:0000041 | ACCEPT | Summary: UniPathway mapping (UPA00193); correct. Supporting Evidence: file:yeast/MIS1/MIS1-uniprot.txt Mitochondrial isozyme of C-1-tetrahydrofolate synthase. The |
| GO:0035999 folate cycle | RCA GO_REF:0000123 | ACCEPT | Summary: YeastPathways RCA (FOLSYN-PWY-1); correct. Supporting Evidence: PMID:3528153 catalyzes 10-formyltetrahydrofolate synthetase (EC 6.3.4.3), 5,10-methenyltetrahydrofolate cyclohydrolase (EC 3.5.4.9), and 5,10-methylenetetrahydrofolate dehydrogenase (EC 1.5.1.5) activities |
| GO:0035999 folate cycle | RCA GO_REF:0000123 | ACCEPT | Summary: YeastPathways RCA (PWY-2201); correct. Supporting Evidence: PMID:3528153 catalyzes 10-formyltetrahydrofolate synthetase (EC 6.3.4.3), 5,10-methenyltetrahydrofolate cyclohydrolase (EC 3.5.4.9), and 5,10-methylenetetrahydrofolate dehydrogenase (EC 1.5.1.5) activities |
| GO:0046417 chorismate metabolic process | RCA GO_REF:0000123 | REMOVE | Summary: YeastPathways RCA inherited from the superpathway of chorismate metabolism (ALL-CHORISMATE-PWY-1), which bundles folate one-carbon reactions with chorismate-derived pathways. Mis1 interconverts one-carbon THF derivatives and does not act on chorismate or any chorismate-derived intermediate. Reason: Superpathway inflation; C1-THF synthase does not participate in chorismate metabolism. Supporting Evidence: PMID:3528153 catalyzes 10-formyltetrahydrofolate synthetase (EC 6.3.4.3), 5,10-methenyltetrahydrofolate cyclohydrolase (EC 3.5.4.9), and 5,10-methylenetetrahydrofolate dehydrogenase (EC 1.5.1.5) activities |
| GO:0046655 folic acid metabolic process | RCA GO_REF:0000123 | MODIFY | Summary: YeastPathways RCA from PWY3O-20 (folate polyglutamylation) to 'folic acid metabolic process', defined as reactions of folic acid itself (pteroylglutamic acid). Mis1 acts on reduced one-carbon THF derivatives; tetrahydrofolate interconversion is the correct term. Reason: Wrong chemical entity (folic acid vs THF derivatives). Proposed replacements: tetrahydrofolate interconversion Supporting Evidence: PMID:3528153 catalyzes 10-formyltetrahydrofolate synthetase (EC 6.3.4.3), 5,10-methenyltetrahydrofolate cyclohydrolase (EC 3.5.4.9), and 5,10-methylenetetrahydrofolate dehydrogenase (EC 1.5.1.5) activities |
| GO:0046655 folic acid metabolic process | RCA GO_REF:0000123 | MODIFY | Summary: YeastPathways RCA from PWY3O-697 (folate interconversions) to 'folic acid metabolic process', defined as reactions of folic acid itself (pteroylglutamic acid). Mis1 acts on reduced one-carbon THF derivatives; tetrahydrofolate interconversion is the correct term. Reason: Wrong chemical entity (folic acid vs THF derivatives). Proposed replacements: tetrahydrofolate interconversion Supporting Evidence: PMID:3528153 catalyzes 10-formyltetrahydrofolate synthetase (EC 6.3.4.3), 5,10-methenyltetrahydrofolate cyclohydrolase (EC 3.5.4.9), and 5,10-methylenetetrahydrofolate dehydrogenase (EC 1.5.1.5) activities |
| GO:0046656 folic acid biosynthetic process | IDA PMID:3528153 Purification and characterization of a mitochondrial isozyme... | MODIFY | Summary: Shannon & Rabinowitz characterised the three C1-THF synthase activities of the mitochondrial isozyme. These interconvert one-carbon units already on THF (formate + THF to 10-formyl-THF to 5,10-methenyl-THF to 5,10-methylene-THF); none synthesises folic acid (pteroylglutamate) de novo, which in yeast is the Fol1/Fol2/Fol3 pathway. The experimental evidence is sound; only the process term is wrong. Reason: The demonstrated activities are tetrahydrofolate interconversions, not folic acid biosynthesis. Proposed replacements: tetrahydrofolate interconversion Supporting Evidence: PMID:3528153 catalyzes 10-formyltetrahydrofolate synthetase (EC 6.3.4.3), 5,10-methenyltetrahydrofolate cyclohydrolase (EC 3.5.4.9), and 5,10-methylenetetrahydrofolate dehydrogenase (EC 1.5.1.5) activities PMID:3528153 Synthetase, cyclohydrolase, and dehydrogenase activities in an ade3 deletion strain co-purify 4,000-fold to yield a single protein species |
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Mitochondrial C1-tetrahydrofolate synthase; involved in interconversion between different oxidation states of tetrahydrofolate (THF); provides activities of formyl-THF synthetase, methenyl-THF cyclohydrolase, and methylene-THF dehydrogenase
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