PET100 encodes a nuclear-encoded assembly chaperone integral to the mitochondrial inner membrane that is required for biogenesis of cytochrome c oxidase (Complex IV). Pet100p specifically interacts with a late-assembly subcomplex containing subunits VII (Cox7p), VIIa (Cox9p), and VIII (Cox8p), facilitating their incorporation into the holoenzyme, but does not remain associated with the fully assembled complex. Loss of Pet100p results in failure to assemble functional cytochrome c oxidase and a respiratory- deficient (petite) phenotype. The protein is conserved in metazoans, and mutations in the human ortholog PET100 cause mitochondrial complex IV deficiency.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
|
GO:0005743
mitochondrial inner membrane
|
IBA
GO_REF:0000033 |
ACCEPT |
Summary: Manual review: mitochondrial inner membrane is consistent with known biology of PET100.
Reason: Retained as supported or plausible for this gene and evidence context.
|
|
GO:0033617
mitochondrial respiratory chain complex IV assembly
|
IBA
GO_REF:0000033 |
ACCEPT |
Summary: Manual review: mitochondrial respiratory chain complex IV assembly is consistent with known biology of PET100.
Reason: Retained as supported or plausible for this gene and evidence context.
|
|
GO:0051082
unfolded protein binding
|
IBA
GO_REF:0000033 |
MARK AS OVER ANNOTATED |
Summary: Manual review: unfolded protein binding is too generic or over-extended for PET100.
Reason: Marked over-annotated because more specific terms capture the biology more accurately.
|
|
GO:0005739
mitochondrion
|
IEA
GO_REF:0000002 |
ACCEPT |
Summary: Manual review: mitochondrion is consistent with known biology of PET100.
Reason: Retained as supported or plausible for this gene and evidence context.
|
|
GO:0031966
mitochondrial membrane
|
IEA
GO_REF:0000044 |
ACCEPT |
Summary: Manual review: mitochondrial membrane is consistent with known biology of PET100.
Reason: Retained as supported or plausible for this gene and evidence context.
|
|
GO:0033617
mitochondrial respiratory chain complex IV assembly
|
IEA
GO_REF:0000002 |
ACCEPT |
Summary: Manual review: mitochondrial respiratory chain complex IV assembly is consistent with known biology of PET100.
Reason: Retained as supported or plausible for this gene and evidence context.
|
|
GO:0005743
mitochondrial inner membrane
|
IDA
PMID:15507444 A role for Pet100p in the assembly of yeast cytochrome c oxi... |
ACCEPT |
Summary: Manual review: mitochondrial inner membrane is consistent with known biology of PET100.
Reason: Retained as supported or plausible for this gene and evidence context.
|
|
GO:0005739
mitochondrion
|
HDA
PMID:16823961 Toward the complete yeast mitochondrial proteome: multidimen... |
ACCEPT |
Summary: Manual review: mitochondrion is consistent with known biology of PET100.
Reason: Retained as supported or plausible for this gene and evidence context.
|
|
GO:0005743
mitochondrial inner membrane
|
IDA
PMID:11498004 Structure and function of Pet100p, a molecular chaperone req... |
ACCEPT |
Summary: Manual review: mitochondrial inner membrane is consistent with known biology of PET100.
Reason: Retained as supported or plausible for this gene and evidence context.
|
|
GO:0033617
mitochondrial respiratory chain complex IV assembly
|
IMP
PMID:11498004 Structure and function of Pet100p, a molecular chaperone req... |
ACCEPT |
Summary: Manual review: mitochondrial respiratory chain complex IV assembly is consistent with known biology of PET100.
Reason: Retained as supported or plausible for this gene and evidence context.
|
|
GO:0033617
mitochondrial respiratory chain complex IV assembly
|
IMP
PMID:15507444 A role for Pet100p in the assembly of yeast cytochrome c oxi... |
ACCEPT |
Summary: Manual review: mitochondrial respiratory chain complex IV assembly is consistent with known biology of PET100.
Reason: Retained as supported or plausible for this gene and evidence context.
|
|
GO:0051082
unfolded protein binding
|
IDA
PMID:11498004 Structure and function of Pet100p, a molecular chaperone req... |
MARK AS OVER ANNOTATED |
Summary: Manual review: unfolded protein binding is too generic or over-extended for PET100.
Reason: Marked over-annotated because more specific terms capture the biology more accurately.
|
id: P38958
gene_symbol: PET100
product_type: PROTEIN
status: DRAFT
taxon:
id: NCBITaxon:559292
label: Saccharomyces cerevisiae
description: >-
PET100 encodes a nuclear-encoded assembly chaperone integral to the
mitochondrial inner membrane that is required for biogenesis of cytochrome c
oxidase (Complex IV). Pet100p specifically interacts with a late-assembly
subcomplex containing subunits VII (Cox7p), VIIa (Cox9p), and VIII (Cox8p),
facilitating their incorporation into the holoenzyme, but does not remain
associated with the fully assembled complex. Loss of Pet100p results in
failure to assemble functional cytochrome c oxidase and a respiratory-
deficient (petite) phenotype. The protein is conserved in metazoans, and
mutations in the human ortholog PET100 cause mitochondrial complex IV
deficiency.
existing_annotations:
- term:
id: GO:0005743
label: mitochondrial inner membrane
evidence_type: IBA
original_reference_id: GO_REF:0000033
review:
summary: 'Manual review: mitochondrial inner membrane is consistent with known biology of PET100.'
action: ACCEPT
reason: Retained as supported or plausible for this gene and evidence context.
- term:
id: GO:0033617
label: mitochondrial respiratory chain complex IV assembly
evidence_type: IBA
original_reference_id: GO_REF:0000033
review:
summary: 'Manual review: mitochondrial respiratory chain complex IV assembly is consistent with known biology of PET100.'
action: ACCEPT
reason: Retained as supported or plausible for this gene and evidence context.
- term:
id: GO:0051082
label: unfolded protein binding
evidence_type: IBA
original_reference_id: GO_REF:0000033
review:
summary: 'Manual review: unfolded protein binding is too generic or over-extended for PET100.'
action: MARK_AS_OVER_ANNOTATED
reason: Marked over-annotated because more specific terms capture the biology more accurately.
- term:
id: GO:0005739
label: mitochondrion
evidence_type: IEA
original_reference_id: GO_REF:0000002
review:
summary: 'Manual review: mitochondrion is consistent with known biology of PET100.'
action: ACCEPT
reason: Retained as supported or plausible for this gene and evidence context.
- term:
id: GO:0031966
label: mitochondrial membrane
evidence_type: IEA
original_reference_id: GO_REF:0000044
review:
summary: 'Manual review: mitochondrial membrane is consistent with known biology of PET100.'
action: ACCEPT
reason: Retained as supported or plausible for this gene and evidence context.
- term:
id: GO:0033617
label: mitochondrial respiratory chain complex IV assembly
evidence_type: IEA
original_reference_id: GO_REF:0000002
review:
summary: 'Manual review: mitochondrial respiratory chain complex IV assembly is consistent with known biology of PET100.'
action: ACCEPT
reason: Retained as supported or plausible for this gene and evidence context.
- term:
id: GO:0005743
label: mitochondrial inner membrane
evidence_type: IDA
original_reference_id: PMID:15507444
review:
summary: 'Manual review: mitochondrial inner membrane is consistent with known biology of PET100.'
action: ACCEPT
reason: Retained as supported or plausible for this gene and evidence context.
- term:
id: GO:0005739
label: mitochondrion
evidence_type: HDA
original_reference_id: PMID:16823961
review:
summary: 'Manual review: mitochondrion is consistent with known biology of PET100.'
action: ACCEPT
reason: Retained as supported or plausible for this gene and evidence context.
- term:
id: GO:0005743
label: mitochondrial inner membrane
evidence_type: IDA
original_reference_id: PMID:11498004
review:
summary: 'Manual review: mitochondrial inner membrane is consistent with known biology of PET100.'
action: ACCEPT
reason: Retained as supported or plausible for this gene and evidence context.
- term:
id: GO:0033617
label: mitochondrial respiratory chain complex IV assembly
evidence_type: IMP
original_reference_id: PMID:11498004
review:
summary: 'Manual review: mitochondrial respiratory chain complex IV assembly is consistent with known biology of PET100.'
action: ACCEPT
reason: Retained as supported or plausible for this gene and evidence context.
- term:
id: GO:0033617
label: mitochondrial respiratory chain complex IV assembly
evidence_type: IMP
original_reference_id: PMID:15507444
review:
summary: 'Manual review: mitochondrial respiratory chain complex IV assembly is consistent with known biology of PET100.'
action: ACCEPT
reason: Retained as supported or plausible for this gene and evidence context.
- term:
id: GO:0051082
label: unfolded protein binding
evidence_type: IDA
original_reference_id: PMID:11498004
review:
summary: 'Manual review: unfolded protein binding is too generic or over-extended for PET100.'
action: MARK_AS_OVER_ANNOTATED
reason: Marked over-annotated because more specific terms capture the biology more accurately.
references:
- id: GO_REF:0000002
title: Gene Ontology annotation through association of InterPro records with GO terms
findings: []
- id: GO_REF:0000033
title: Annotation inferences using phylogenetic trees
findings: []
- id: GO_REF:0000044
title: Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
findings: []
- id: PMID:11498004
title: Structure and function of Pet100p, a molecular chaperone required for the assembly of cytochrome c oxidase in Saccharomyces cerevisiae.
findings: []
- id: PMID:15507444
title: 'A role for Pet100p in the assembly of yeast cytochrome c oxidase: interaction with a subassembly that accumulates in a pet100 mutant.'
findings: []
- id: PMID:16823961
title: 'Toward the complete yeast mitochondrial proteome: multidimensional separation techniques for mitochondrial proteomics.'
findings: []