PGS1

UniProt ID: P25578
Organism: Saccharomyces cerevisiae S288C
Review Status: COMPLETE
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Gene Description

Pgs1 (Pel1, YCL004W) is the CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase (phosphatidylglycerophosphate synthase, EC 2.7.8.5) of Saccharomyces cerevisiae, a phospholipase D-superfamily (HKD motif) enzyme of the mitochondrial inner membrane. It condenses CDP-diacylglycerol with sn-glycerol 3-phosphate to form phosphatidylglycerophosphate, the committed and rate-limiting step of phosphatidylglycerol and cardiolipin synthesis inside mitochondria (followed by Gep4 and Crd1). pgs1 null cells lack PG and CL, are temperature sensitive, cannot grow on non-fermentable carbon sources and are petite-lethal; PGS1 expression is regulated by inositol and choline through the Ino2/Ino4-Opi1 circuit.

Existing Annotations Review

GO Term Evidence Action Reason
GO:0003824 catalytic activity
IEA
GO_REF:0000002 		MODIFY
Summary: InterPro IPR001736 (PLD/transphosphatidylase) to the root term catalytic activity. Uninformative.
Reason: Replace with the specific PGP synthase activity.
Supporting Evidence:
PMID:9545322
Overexpression of the PGS1 gene product under the inducible GAL1 promoter resulted in a 14-fold increase in in vitro PG-P synthase activity.
GO:0005739 mitochondrion
IBA
GO_REF:0000033 		ACCEPT
Summary: IBA is_active_in mitochondrion from PTN000287047 (PGS1 orthologs incl. S. pombe, trypanosome, human). Correct.
Supporting Evidence:
PMID:9799363
its fluorescent protein was localized to mitochondria
GO:0005739 mitochondrion
IDA
PMID:9799363
Phosphatidylglycerolphosphate synthase encoded by the PEL1/P... 		ACCEPT
Summary: Pel1-GFP (functional) localized to mitochondria.
Supporting Evidence:
PMID:9799363
its fluorescent protein was localized to mitochondria
GO:0005739 mitochondrion
IEA
GO_REF:0000120 		ACCEPT
Summary: ARBA/UniProt subcellular location mapping to mitochondrion. Correct.
Supporting Evidence:
PMID:9799363
its fluorescent protein was localized to mitochondria
GO:0005739 mitochondrion
RCA
GO_REF:0000123 		ACCEPT
Summary: YeastPathways PHOS-PWY places the PGP synthase step in mitochondria. Correct (inner membrane activity).
Supporting Evidence:
PMID:3005242
Phosphatidylserine decarboxylase and phosphatidylglycerolphosphate synthase were localized exclusively in the inner mitochondrial membrane
GO:0005829 cytosol
RCA
GO_REF:0000123 		REMOVE
Summary: YeastPathways PHOSLIPSYN2-PWY-1 / PWY3O-2 RCA places the step in the cytosol. Pgs1 is a mitochondrial inner-membrane enzyme; cytosol is wrong and contradicts the other RCA row (mitochondrion).
Reason: Localization default of the pathway conversion; contradicted by IDA mitochondrial localization.
Supporting Evidence:
PMID:9799363
its fluorescent protein was localized to mitochondria
PMID:3005242
Phosphatidylserine decarboxylase and phosphatidylglycerolphosphate synthase were localized exclusively in the inner mitochondrial membrane
GO:0005829 cytosol
RCA
GO_REF:0000123 		REMOVE
Summary: YeastPathways PHOSLIPSYN2-PWY-1 / PWY3O-2 RCA places the step in the cytosol. Pgs1 is a mitochondrial inner-membrane enzyme; cytosol is wrong and contradicts the other RCA row (mitochondrion).
Reason: Localization default of the pathway conversion; contradicted by IDA mitochondrial localization.
Supporting Evidence:
PMID:9799363
its fluorescent protein was localized to mitochondria
PMID:3005242
Phosphatidylserine decarboxylase and phosphatidylglycerolphosphate synthase were localized exclusively in the inner mitochondrial membrane
GO:0006655 phosphatidylglycerol biosynthetic process
IEA
GO_REF:0000041 		ACCEPT
Summary: UniPathway UPA00084 (PG from CDP-DAG, step 1/2) mapping. pgs1 null lacks PG. Correct.
Supporting Evidence:
PMID:9545322
The pgs1 null mutant exhibited no detectable in vitro PG-P synthase activity and no detectable CL or phosphatidylglycerol (PG); significant CL synthase activity was still present.
GO:0008444 CDP-diacylglycerol-glycerol-3-phosphate 3-phosphatidyltransferase activity
IBA
GO_REF:0000033 		ACCEPT
Summary: IBA from PTN000287047. Core activity.
Supporting Evidence:
PMID:9545322
Overexpression of the PGS1 gene product under the inducible GAL1 promoter resulted in a 14-fold increase in in vitro PG-P synthase activity.
PMID:9545322
These results unequivocally demonstrate that the PGS1 gene encodes the major PG-P synthase of yeast
GO:0008444 CDP-diacylglycerol-glycerol-3-phosphate 3-phosphatidyltransferase activity
IEA
GO_REF:0000120 		ACCEPT
Summary: Combined IEA from ARBA/InterPro IPR016270/RHEA:12593/EC 2.7.8.5. Correct.
Supporting Evidence:
PMID:9545322
These results unequivocally demonstrate that the PGS1 gene encodes the major PG-P synthase of yeast
GO:0008444 CDP-diacylglycerol-glycerol-3-phosphate 3-phosphatidyltransferase activity
IMP
PMID:9545322
The PEL1 gene (renamed PGS1) encodes the phosphatidylglycero... 		ACCEPT
Summary: pgs1 null has no detectable PGP synthase activity; overexpression raises activity 14-fold. Core activity.
Supporting Evidence:
PMID:9545322
Overexpression of the PGS1 gene product under the inducible GAL1 promoter resulted in a 14-fold increase in in vitro PG-P synthase activity.
PMID:9545322
The pgs1 null mutant exhibited no detectable in vitro PG-P synthase activity and no detectable CL or phosphatidylglycerol (PG); significant CL synthase activity was still present.
GO:0008444 CDP-diacylglycerol-glycerol-3-phosphate 3-phosphatidyltransferase activity
RCA
GO_REF:0000123 		ACCEPT
Summary: YeastPathways assigns Pgs1 to CDP-DAG + glycerol-3-P -> PGP + CMP (EC 2.7.8.5). Correct.
Supporting Evidence:
PMID:9545322
These results unequivocally demonstrate that the PGS1 gene encodes the major PG-P synthase of yeast
GO:0008444 CDP-diacylglycerol-glycerol-3-phosphate 3-phosphatidyltransferase activity
RCA
GO_REF:0000123 		ACCEPT
Summary: YeastPathways assigns Pgs1 to CDP-DAG + glycerol-3-P -> PGP + CMP (EC 2.7.8.5). Correct.
Supporting Evidence:
PMID:9545322
These results unequivocally demonstrate that the PGS1 gene encodes the major PG-P synthase of yeast
GO:0008444 CDP-diacylglycerol-glycerol-3-phosphate 3-phosphatidyltransferase activity
RCA
GO_REF:0000123 		ACCEPT
Summary: YeastPathways assigns Pgs1 to CDP-DAG + glycerol-3-P -> PGP + CMP (EC 2.7.8.5). Correct.
Supporting Evidence:
PMID:9545322
These results unequivocally demonstrate that the PGS1 gene encodes the major PG-P synthase of yeast
GO:0008654 phospholipid biosynthetic process
RCA
GO_REF:0000123 		TOO GENERAL
Summary: YeastPathways superpathway RCA to phospholipid biosynthetic process. Correct general term.
Reason: True, but entailed by the more specific GO:0006655 phosphatidylglycerol biosynthetic process and GO:0032049 cardiolipin biosynthetic process, accepted in this review.
Supporting Evidence:
PMID:9545322
functions as the committed and rate-limiting step in the biosynthesis of cardiolipin (CL)
GO:0008654 phospholipid biosynthetic process
RCA
GO_REF:0000123 		TOO GENERAL
Summary: YeastPathways superpathway RCA to phospholipid biosynthetic process. Correct general term.
Reason: True, but entailed by the more specific GO:0006655 phosphatidylglycerol biosynthetic process and GO:0032049 cardiolipin biosynthetic process, accepted in this review.
Supporting Evidence:
PMID:9545322
functions as the committed and rate-limiting step in the biosynthesis of cardiolipin (CL)
GO:0008654 phospholipid biosynthetic process
RCA
GO_REF:0000123 		TOO GENERAL
Summary: YeastPathways superpathway RCA to phospholipid biosynthetic process. Correct general term.
Reason: True, but entailed by the more specific GO:0006655 phosphatidylglycerol biosynthetic process and GO:0032049 cardiolipin biosynthetic process, accepted in this review.
Supporting Evidence:
PMID:9545322
functions as the committed and rate-limiting step in the biosynthesis of cardiolipin (CL)
GO:0032049 cardiolipin biosynthetic process
IBA
GO_REF:0000033 		ACCEPT
Summary: IBA from PTN000287047. PGP synthase is the committed step of cardiolipin synthesis.
Supporting Evidence:
PMID:9545322
functions as the committed and rate-limiting step in the biosynthesis of cardiolipin (CL)
GO:0032049 cardiolipin biosynthetic process
IEA
GO_REF:0000120 		ACCEPT
Summary: ARBA/InterPro IPR016270 mapping. Correct.
Supporting Evidence:
PMID:9545322
functions as the committed and rate-limiting step in the biosynthesis of cardiolipin (CL)
GO:0032049 cardiolipin biosynthetic process
IMP
PMID:9545322
The PEL1 gene (renamed PGS1) encodes the phosphatidylglycero... 		ACCEPT
Summary: pgs1 null has no detectable cardiolipin.
Supporting Evidence:
PMID:9545322
The pgs1 null mutant exhibited no detectable in vitro PG-P synthase activity and no detectable CL or phosphatidylglycerol (PG); significant CL synthase activity was still present.
GO:0005743 mitochondrial inner membrane
IC
PMID:3005242
Subcellular and submitochondrial localization of phospholipi... 		NEW
Summary: Submitochondrial fractionation of yeast mitochondria places phosphatidylglycerolphosphate synthase activity exclusively in the inner membrane.
Reason: Per the organelle policy the mitochondrion rows are kept and the sub-compartment is added beside them. This is a curator inference (IC): PMID:3005242 (1986) localized PGP synthase activity to the inner membrane before the gene was identified, and PGS1 was later shown to encode essentially all PGP synthase activity (PMID:9545322); the inner-membrane location of the downstream cardiolipin synthase CRD1 agrees. The core_functions location already uses this term.
Supporting Evidence:
PMID:9545322
The pgs1 null mutant exhibited no detectable in vitro PG-P synthase activity
PMID:3005242
Phosphatidylserine decarboxylase and phosphatidylglycerolphosphate synthase were localized exclusively in the inner mitochondrial membrane

Core Functions

Condensation of CDP-diacylglycerol with sn-glycerol 3-phosphate to phosphatidylglycerophosphate, the committed step of phosphatidylglycerol and cardiolipin synthesis in the mitochondrial inner membrane.

Molecular Function:
CDP-diacylglycerol-glycerol-3-phosphate 3-phosphatidyltransferase activity
Directly Involved In:
cardiolipin biosynthetic process phosphatidylglycerol biosynthetic process
Cellular Locations:
mitochondrial inner membrane
Supporting Evidence:
  • PMID:9545322
    Overexpression of the PGS1 gene product under the inducible GAL1 promoter resulted in a 14-fold increase in in vitro PG-P synthase activity.
  • PMID:9545322
    The pgs1 null mutant exhibited no detectable in vitro PG-P synthase activity and no detectable CL or phosphatidylglycerol (PG); significant CL synthase activity was still present.
  • PMID:3005242
    Phosphatidylserine decarboxylase and phosphatidylglycerolphosphate synthase were localized exclusively in the inner mitochondrial membrane

References

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Deep Research

SGD

(PGS1-deep-research-sgd.md)
SGD gene description for PGS1 (yeast) SGD

SGD gene description for PGS1 (yeast)

Phosphatidylglycerolphosphate synthase; catalyzes the synthesis of phosphatidylglycerolphosphate from CDP-diacylglycerol and sn-glycerol 3-phosphate in the first committed and rate-limiting step of cardiolipin biosynthesis

📚 Additional Documentation

Notes

(PGS1-notes.md)

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