SAS3

UniProt ID: P34218
Organism: Saccharomyces cerevisiae
Review Status: COMPLETE
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Gene Description

Histone acetyltransferase SAS3 is the catalytic subunit of the NuA3 histone acetyltransferase complex.

Proposed New Ontology Terms

histone H3K14 acetyltransferase activity

Definition: Catalysis of acetylation of histone H3 at lysine 14.

Justification: Critical substrate-specific function documented in literature.

Existing Annotations Review

GO Term Evidence Action Reason
GO:0000781 chromosome, telomeric region
IEA
GO_REF:0000108 		KEEP AS NON CORE
Summary: Telomeric-region localization is plausible but secondary to SAS3 catalytic and complex-level functions.
Reason: Kept as non-core because SAS3 has reported chromatin-region associations, but this location term is peripheral compared with its core HAT activity in NuA3 complexes.
GO:0000785 chromatin
IBA
GO_REF:0000033 		ACCEPT
Summary: chromatin is consistent with curated evidence for SAS3 function in NuA3-associated chromatin acetylation.
Reason: Accepted as concordant with SAS3 molecular role and literature-supported complex/process context.
GO:0003682 chromatin binding
IBA
GO_REF:0000033 		ACCEPT
Summary: chromatin binding is consistent with curated evidence for SAS3 function in NuA3-associated chromatin acetylation.
Reason: Accepted as concordant with SAS3 molecular role and literature-supported complex/process context.
GO:0003712 transcription coregulator activity
IBA
GO_REF:0000033 		ACCEPT
Summary: transcription coregulator activity is consistent with curated evidence for SAS3 function in NuA3-associated chromatin acetylation.
Reason: Accepted as concordant with SAS3 molecular role and literature-supported complex/process context.
GO:0004402 histone acetyltransferase activity
IBA
GO_REF:0000033 		ACCEPT
Summary: histone acetyltransferase activity is consistent with curated evidence for SAS3 function in NuA3-associated chromatin acetylation.
Reason: Accepted as concordant with SAS3 molecular role and literature-supported complex/process context.
GO:0005515 protein binding
IPI
PMID:12077334
Yng1p modulates the activity of Sas3p as a component of the ... 		REMOVE
Summary: Generic protein binding is not sufficiently informative for SAS3 curation.
Reason: Removed because SAS3 interaction biology is better captured by specific NuA3 complex and histone-acetyltransferase annotations than by broad GO:0005515.
Supporting Evidence:
PMID:12077334
Yng1p modulates the activity of Sas3p as a component of the yeast NuA3 Hhistone acetyltransferase complex.
GO:0005634 nucleus
IBA
GO_REF:0000033 		ACCEPT
Summary: nucleus is consistent with curated evidence for SAS3 function in NuA3-associated chromatin acetylation.
Reason: Accepted as concordant with SAS3 molecular role and literature-supported complex/process context.
GO:0006325 chromatin organization
IEA
GO_REF:0000043 		ACCEPT
Summary: chromatin organization is consistent with curated evidence for SAS3 function in NuA3-associated chromatin acetylation.
Reason: Accepted as concordant with SAS3 molecular role and literature-supported complex/process context.
GO:0006351 DNA-templated transcription
IEA
GO_REF:0000043 		MARK AS OVER ANNOTATED
Summary: This process term is broader than the specific mechanistic role supported for SAS3.
Reason: Marked as over-annotated to favor more specific SAS3 functions (histone acetyltransferase activity, NuA3 complex membership, and transcription-regulatory process terms).
GO:0006355 regulation of DNA-templated transcription
IEA
GO_REF:0000002 		ACCEPT
Summary: regulation of DNA-templated transcription is consistent with curated evidence for SAS3 function in NuA3-associated chromatin acetylation.
Reason: Accepted as concordant with SAS3 molecular role and literature-supported complex/process context.
GO:0006357 regulation of transcription by RNA polymerase II
IBA
GO_REF:0000033 		ACCEPT
Summary: regulation of transcription by RNA polymerase II is consistent with curated evidence for SAS3 function in NuA3-associated chromatin acetylation.
Reason: Accepted as concordant with SAS3 molecular role and literature-supported complex/process context.
GO:0008270 zinc ion binding
IEA
GO_REF:0000043 		ACCEPT
Summary: zinc ion binding is consistent with curated evidence for SAS3 function in NuA3-associated chromatin acetylation.
Reason: Accepted as concordant with SAS3 molecular role and literature-supported complex/process context.
GO:0016740 transferase activity
IEA
GO_REF:0000043 		ACCEPT
Summary: transferase activity is consistent with curated evidence for SAS3 function in NuA3-associated chromatin acetylation.
Reason: Accepted as concordant with SAS3 molecular role and literature-supported complex/process context.
GO:0016746 acyltransferase activity
IEA
GO_REF:0000043 		ACCEPT
Summary: acyltransferase activity is consistent with curated evidence for SAS3 function in NuA3-associated chromatin acetylation.
Reason: Accepted as concordant with SAS3 molecular role and literature-supported complex/process context.
GO:0030466 silent mating-type cassette heterochromatin formation
IMP
PMID:11731479
The yeast SAS (something about silencing) protein complex co... 		ACCEPT
Summary: silent mating-type cassette heterochromatin formation is consistent with curated evidence for SAS3 function in NuA3-associated chromatin acetylation.
Reason: Accepted as concordant with SAS3 molecular role and literature-supported complex/process context.
Supporting Evidence:
PMID:11731479
The yeast SAS (something about silencing) protein complex contains a MYST-type putative acetyltransferase and functions with chromatin assembly factor ASF1.
GO:0031507 heterochromatin formation
IEA
GO_REF:0000117 		MARK AS OVER ANNOTATED
Summary: This process term is broader than the specific mechanistic role supported for SAS3.
Reason: Marked as over-annotated to favor more specific SAS3 functions (histone acetyltransferase activity, NuA3 complex membership, and transcription-regulatory process terms).
GO:0031509 subtelomeric heterochromatin formation
IMP
PMID:11731479
The yeast SAS (something about silencing) protein complex co... 		ACCEPT
Summary: subtelomeric heterochromatin formation is consistent with curated evidence for SAS3 function in NuA3-associated chromatin acetylation.
Reason: Accepted as concordant with SAS3 molecular role and literature-supported complex/process context.
Supporting Evidence:
PMID:11731479
The yeast SAS (something about silencing) protein complex contains a MYST-type putative acetyltransferase and functions with chromatin assembly factor ASF1.
GO:0033100 NuA3 histone acetyltransferase complex
IDA
PMID:10817755
The something about silencing protein, Sas3, is the catalyti... 		ACCEPT
Summary: NuA3 histone acetyltransferase complex is consistent with curated evidence for SAS3 function in NuA3-associated chromatin acetylation.
Reason: Accepted as concordant with SAS3 molecular role and literature-supported complex/process context.
Supporting Evidence:
PMID:10817755
The something about silencing protein, Sas3, is the catalytic subunit of NuA3, a yTAF(II)30-containing HAT complex that interacts with the Spt16 subunit of the yeast CP (Cdc68/Pob3)-FACT complex.
GO:0046872 metal ion binding
IEA
GO_REF:0000043 		ACCEPT
Summary: metal ion binding is consistent with curated evidence for SAS3 function in NuA3-associated chromatin acetylation.
Reason: Accepted as concordant with SAS3 molecular role and literature-supported complex/process context.
GO:0061733 protein-lysine-acetyltransferase activity
IEA
GO_REF:0000120 		ACCEPT
Summary: protein-lysine-acetyltransferase activity is consistent with curated evidence for SAS3 function in NuA3-associated chromatin acetylation.
Reason: Accepted as concordant with SAS3 molecular role and literature-supported complex/process context.
GO:0070775 H3 histone acetyltransferase complex
IEA
GO_REF:0000117 		ACCEPT
Summary: H3 histone acetyltransferase complex is consistent with curated evidence for SAS3 function in NuA3-associated chromatin acetylation.
Reason: Accepted as concordant with SAS3 molecular role and literature-supported complex/process context.
GO:1990467 NuA3a histone acetyltransferase complex
IBA
GO_REF:0000033 		ACCEPT
Summary: NuA3a histone acetyltransferase complex is consistent with curated evidence for SAS3 function in NuA3-associated chromatin acetylation.
Reason: Accepted as concordant with SAS3 molecular role and literature-supported complex/process context.
GO:1990468 NuA3b histone acetyltransferase complex
IDA
PMID:25104842
A PWWP domain-containing protein targets the NuA3 acetyltran... 		ACCEPT
Summary: NuA3b histone acetyltransferase complex is consistent with curated evidence for SAS3 function in NuA3-associated chromatin acetylation.
Reason: Accepted as concordant with SAS3 molecular role and literature-supported complex/process context.
Supporting Evidence:
PMID:25104842
Epub 2014 Aug 6. A PWWP domain-containing protein targets the NuA3 acetyltransferase complex via histone H3 lysine 36 trimethylation to coordinate transcriptional elongation at coding regions.
GO:0004402 histone acetyltransferase activity
IEA
GO_REF:0000120 		ACCEPT
Summary: histone acetyltransferase activity is consistent with curated evidence for SAS3 function in NuA3-associated chromatin acetylation.
Reason: Accepted as concordant with SAS3 molecular role and literature-supported complex/process context.
GO:0005634 nucleus
IEA
GO_REF:0000044 		ACCEPT
Summary: nucleus is consistent with curated evidence for SAS3 function in NuA3-associated chromatin acetylation.
Reason: Accepted as concordant with SAS3 molecular role and literature-supported complex/process context.
GO:0005515 protein binding
IPI
PMID:12672825
Opposite role of yeast ING family members in p53-dependent t... 		REMOVE
Summary: Generic protein binding is not sufficiently informative for SAS3 curation.
Reason: Removed because SAS3 interaction biology is better captured by specific NuA3 complex and histone-acetyltransferase annotations than by broad GO:0005515.
Supporting Evidence:
PMID:12672825
2003 Apr 2. Opposite role of yeast ING family members in p53-dependent transcriptional activation.
GO:0005515 protein binding
IPI
PMID:16554755
Global landscape of protein complexes in the yeast Saccharom... 		REMOVE
Summary: Generic protein binding is not sufficiently informative for SAS3 curation.
Reason: Removed because SAS3 interaction biology is better captured by specific NuA3 complex and histone-acetyltransferase annotations than by broad GO:0005515.
Supporting Evidence:
PMID:16554755
Global landscape of protein complexes in the yeast Saccharomyces cerevisiae.
GO:0005515 protein binding
IPI
PMID:17157260
Yng1 PHD finger binding to H3 trimethylated at K4 promotes N... 		REMOVE
Summary: Generic protein binding is not sufficiently informative for SAS3 curation.
Reason: Removed because SAS3 interaction biology is better captured by specific NuA3 complex and histone-acetyltransferase annotations than by broad GO:0005515.
Supporting Evidence:
PMID:17157260
Yng1 PHD finger binding to H3 trimethylated at K4 promotes NuA3 HAT activity at K14 of H3 and transcription at a subset of targeted ORFs.
GO:0005515 protein binding
IPI
PMID:21179020
Defining the budding yeast chromatin-associated interactome. 		REMOVE
Summary: Generic protein binding is not sufficiently informative for SAS3 curation.
Reason: Removed because SAS3 interaction biology is better captured by specific NuA3 complex and histone-acetyltransferase annotations than by broad GO:0005515.
Supporting Evidence:
PMID:21179020
Defining the budding yeast chromatin-associated interactome.
GO:0005515 protein binding
IPI
PMID:25473596
Comprehensive analysis of interacting proteins and genome-wi... 		REMOVE
Summary: Generic protein binding is not sufficiently informative for SAS3 curation.
Reason: Removed because SAS3 interaction biology is better captured by specific NuA3 complex and histone-acetyltransferase annotations than by broad GO:0005515.
Supporting Evidence:
PMID:25473596
eCollection 2014. Comprehensive analysis of interacting proteins and genome-wide location studies of the Sas3-dependent NuA3 histone acetyltransferase complex.
GO:0005515 protein binding
IPI
PMID:37968396
The social and structural architecture of the yeast protein ... 		REMOVE
Summary: Generic protein binding is not sufficiently informative for SAS3 curation.
Reason: Removed because SAS3 interaction biology is better captured by specific NuA3 complex and histone-acetyltransferase annotations than by broad GO:0005515.
Supporting Evidence:
PMID:37968396
Nov 15. The social and structural architecture of the yeast protein interactome.
GO:0005634 nucleus
NAS
PMID:17157260
Yng1 PHD finger binding to H3 trimethylated at K4 promotes N... 		ACCEPT
Summary: nucleus is consistent with curated evidence for SAS3 function in NuA3-associated chromatin acetylation.
Reason: Accepted as concordant with SAS3 molecular role and literature-supported complex/process context.
Supporting Evidence:
PMID:17157260
Yng1 PHD finger binding to H3 trimethylated at K4 promotes NuA3 HAT activity at K14 of H3 and transcription at a subset of targeted ORFs.
GO:0006351 DNA-templated transcription
NAS
PMID:17157260
Yng1 PHD finger binding to H3 trimethylated at K4 promotes N... 		MARK AS OVER ANNOTATED
Summary: This process term is broader than the specific mechanistic role supported for SAS3.
Reason: Marked as over-annotated to favor more specific SAS3 functions (histone acetyltransferase activity, NuA3 complex membership, and transcription-regulatory process terms).
Supporting Evidence:
PMID:17157260
Yng1 PHD finger binding to H3 trimethylated at K4 promotes NuA3 HAT activity at K14 of H3 and transcription at a subset of targeted ORFs.
GO:0008270 zinc ion binding
RCA
PMID:30358795
The cellular economy of the Saccharomyces cerevisiae zinc pr... 		ACCEPT
Summary: zinc ion binding is consistent with curated evidence for SAS3 function in NuA3-associated chromatin acetylation.
Reason: Accepted as concordant with SAS3 molecular role and literature-supported complex/process context.
Supporting Evidence:
PMID:30358795
The cellular economy of the Saccharomyces cerevisiae zinc proteome.
GO:1990467 NuA3a histone acetyltransferase complex
IDA
PMID:25104842
A PWWP domain-containing protein targets the NuA3 acetyltran... 		ACCEPT
Summary: NuA3a histone acetyltransferase complex is consistent with curated evidence for SAS3 function in NuA3-associated chromatin acetylation.
Reason: Accepted as concordant with SAS3 molecular role and literature-supported complex/process context.
Supporting Evidence:
PMID:25104842
Epub 2014 Aug 6. A PWWP domain-containing protein targets the NuA3 acetyltransferase complex via histone H3 lysine 36 trimethylation to coordinate transcriptional elongation at coding regions.
GO:0004402 histone acetyltransferase activity
IDA
PMID:10817755
The something about silencing protein, Sas3, is the catalyti... 		ACCEPT
Summary: histone acetyltransferase activity is consistent with curated evidence for SAS3 function in NuA3-associated chromatin acetylation.
Reason: Accepted as concordant with SAS3 molecular role and literature-supported complex/process context.
Supporting Evidence:
PMID:10817755
The something about silencing protein, Sas3, is the catalytic subunit of NuA3, a yTAF(II)30-containing HAT complex that interacts with the Spt16 subunit of the yeast CP (Cdc68/Pob3)-FACT complex.
GO:0004402 histone acetyltransferase activity
IMP
PMID:10817755
The something about silencing protein, Sas3, is the catalyti... 		ACCEPT
Summary: histone acetyltransferase activity is consistent with curated evidence for SAS3 function in NuA3-associated chromatin acetylation.
Reason: Accepted as concordant with SAS3 molecular role and literature-supported complex/process context.
Supporting Evidence:
PMID:10817755
The something about silencing protein, Sas3, is the catalytic subunit of NuA3, a yTAF(II)30-containing HAT complex that interacts with the Spt16 subunit of the yeast CP (Cdc68/Pob3)-FACT complex.
GO:0033100 NuA3 histone acetyltransferase complex
IDA
PMID:17157260
Yng1 PHD finger binding to H3 trimethylated at K4 promotes N... 		ACCEPT
Summary: NuA3 histone acetyltransferase complex is consistent with curated evidence for SAS3 function in NuA3-associated chromatin acetylation.
Reason: Accepted as concordant with SAS3 molecular role and literature-supported complex/process context.
Supporting Evidence:
PMID:17157260
Yng1 PHD finger binding to H3 trimethylated at K4 promotes NuA3 HAT activity at K14 of H3 and transcription at a subset of targeted ORFs.

Core Functions

SAS3 catalyzes histone H3K14 acetylation in NuA3a and NuA3b complexes.

Molecular Function:
histone acetyltransferase activity
Supporting Evidence:

References

GO_REF:0000002
Gene Ontology annotation through association of InterPro records with GO terms
GO_REF:0000033
Annotation inferences using phylogenetic trees
GO_REF:0000043
Gene Ontology annotation based on UniProtKB/Swiss-Prot keyword mapping
GO_REF:0000108
Automatic assignment of GO terms using logical inference, based on on inter-ontology links
GO_REF:0000117
Electronic Gene Ontology annotations created by ARBA machine learning models
GO_REF:0000120
Combined Automated Annotation using Multiple IEA Methods
PMID:10817755
The something about silencing protein, Sas3, is the catalytic subunit of NuA3, a yTAF(II)30-containing HAT complex that interacts with the Spt16 subunit of the yeast CP (Cdc68/Pob3)-FACT complex.
PMID:11731479
The yeast SAS (something about silencing) protein complex contains a MYST-type putative acetyltransferase and functions with chromatin assembly factor ASF1.
PMID:12077334
Yng1p modulates the activity of Sas3p as a component of the yeast NuA3 Hhistone acetyltransferase complex.
PMID:17157260
Yng1 PHD finger binding to H3 trimethylated at K4 promotes NuA3 HAT activity at K14 of H3 and transcription at a subset of targeted ORFs.
PMID:25104842
A PWWP domain-containing protein targets the NuA3 acetyltransferase complex via histone H3 lysine 36 trimethylation to coordinate transcriptional elongation at coding regions.
GO_REF:0000044
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
PMID:12672825
Opposite role of yeast ING family members in p53-dependent transcriptional activation.
PMID:16554755
Global landscape of protein complexes in the yeast Saccharomyces cerevisiae.
PMID:21179020
Defining the budding yeast chromatin-associated interactome.
PMID:25473596
Comprehensive analysis of interacting proteins and genome-wide location studies of the Sas3-dependent NuA3 histone acetyltransferase complex.
PMID:30358795
The cellular economy of the Saccharomyces cerevisiae zinc proteome.
PMID:37968396
The social and structural architecture of the yeast protein interactome.

Suggested Questions for Experts

Q: Does SAS3 have in vivo role in H4 acetylation?

Q: What is relative contribution of SAS3 to total H3K14ac?

Q: What distinguishes NuA3a vs NuA3b targeting?

Q: Does SAS3 interact with HDAC complexes?

Suggested Experiments

Experiment: Substrate specificity assays with histone variants

Experiment: Genome-wide ChIP-seq for SAS3 and H3K14ac

Experiment: Activity analysis in cells lacking GCN5

Experiment: Test interactions with HDAC complexes

📚 Additional Documentation

Curation Summary

(SAS3-CURATION-SUMMARY.md)

SAS3 Gene Annotation Review - Curation Summary

Gene: SAS3 (Histone acetyltransferase SAS3)
UniProt ID: P34218
Organism: Saccharomyces cerevisiae
Review Date: 2025-12-31

Executive Summary

Reviewed 38 GO annotations for yeast SAS3, the catalytic subunit of the NuA3 histone acetyltransferase complex. SAS3 is fundamentally different from its paralog SAS2:
- SAS3 primarily regulates transcription activation via H3K14 acetylation
- SAS2 primarily mediates transcriptional silencing via H4K16 acetylation

Key Findings

Substrate Specificity (Critical)

Primary substrate: Histone H3K14 (NOT H3K9)
- Documented in UniProt function field
- Confirmed by PMID:10817755, PMID:17157260, PMID:25104842
- In vitro also acetylates free histones H3 and H4

Complex Organization

SAS3 functions in TWO distinct NuA3 forms:
1. NuA3a - binds H3K4me3 at promoters → transcription initiation
- Composition: NTO1, SAS3, TAF14, YNG1, EAF6
- Evidence: PMID:17157260 (PMID:25104842)
2. NuA3b - binds H3K36me3 at coding regions → transcription elongation
- Unique subunit: PDP3 (PWWP domain)
- Evidence: PMID:25104842

Annotation Actions Summary

Annotations ACCEPTED (27)

Well-supported, mechanistically accurate:
- GO:0000785 (chromatin) - IBA
- GO:0004402 (histone acetyltransferase activity) - IBA, IEA, IDA, IMP
- GO:0005634 (nucleus) - IBA, IEA, NAS
- GO:0006357 (regulation of transcription by RNA polymerase II) - IBA
- GO:0003682 (chromatin binding) - IBA
- GO:1990467 (NuA3a histone acetyltransferase complex) - IBA, IDA
- GO:1990468 (NuA3b histone acetyltransferase complex) - IDA
- GO:0003712 (transcription coregulator activity) - IBA
- GO:0006325 (chromatin organization) - IEA
- GO:0006355 (regulation of DNA-templated transcription) - IEA
- GO:0008270 (zinc ion binding) - IEA, RCA
- GO:0016740 (transferase activity) - IEA
- GO:0016746 (acyltransferase activity) - IEA
- GO:0046872 (metal ion binding) - IEA
- GO:0061733 (protein-lysine-acetyltransferase activity) - IEA
- GO:0070775 (H3 histone acetyltransferase complex) - IEA
- GO:0031509 (subtelomeric heterochromatin formation) - IMP
- GO:0030466 (silent mating-type cassette heterochromatin formation) - IMP
- GO:0033100 (NuA3 histone acetyltransferase complex) - IDA (×2)

Annotations REMOVED (7)

Generic "protein binding" (GO:0005515) with IPI evidence
- PMID:12077334, PMID:12672825, PMID:16554755, PMID:17157260, PMID:21179020, PMID:25473596, PMID:37968396

Reason: GO:0005515 is minimally informative and violates GO annotation guidelines. The specific functionally-relevant interactions (SAS3-YNG1, SAS3-TAF14, SAS3-SPT16) are better captured by complex membership annotations (GO:0033100, GO:1990467, GO:1990468) which provide proper mechanistic context.

Annotations MARKED AS OVER-ANNOTATED (2)

  • GO:0006351 (DNA-templated transcription) - IEA, NAS
  • Mechanistically misleading: SAS3 regulates transcription but doesn't catalyze it directly

  • Better terms: GO:0006357 (regulation), GO:0031509 (heterochromatin formation)

  • GO:0031507 (heterochromatin formation) - IEA

  • Secondary to primary transcription activation role
  • More specific terms available: GO:0031509, GO:0030466

Annotations MARKED AS NON-CORE (2)

  • GO:0000781 (chromosome, telomeric region) - IEA
  • Technically sound logical inference but too general
  • More specific functional terms more informative

Missing Annotations (NEW)

GO:0036408 - histone H3K14 acetyltransferase activity
- Evidence: IDA (Direct Assay)
- References: PMID:10817755, PMID:17157260
- Justification: This substrate-specific function is critical to SAS3 and well-documented but missing from annotations. Parallel to GO:0046972 (H4K16 acetyltransferase) for related HATs.

Core Functions Identified

  1. Histone H3K14 Acetylation (primary molecular function)
  2. Catalytic activity of SAS3 in both NuA3a and NuA3b

  3. Essential for transcription regulation

  4. Transcription Initiation (NuA3a)

  5. H3K4me3 recognition → H3K14 acetylation at promoters

  6. YNG1 PHD finger coordinates targeting

  7. Transcription Elongation (NuA3b)

  8. H3K36me3 recognition → H3K14 acetylation at coding regions

  9. PDP3 PWWP domain coordinates targeting

  10. Heterochromatin Formation (secondary)

  11. HML silencing and subtelomeric heterochromatin (IMP evidence)
  12. Less important than transcription activation roles

Comparison with SAS2 Paralog

Feature SAS3 SAS2
Complex NuA3 (two forms) SAS (SAS2/SAS4/SAS5)
Primary Substrate H3K14 H4K16 + H3K14
Primary Function Transcription activation Transcriptional silencing
Key Histone Mark H3K4me3, H3K36me3 Telomeric/subtelomeric regions
Secondary Function HML silencing Limited

Literature Evidence Summary

Foundational Papers:
- PMID:10817755 - Discovery of SAS3 as NuA3 catalytic subunit; biochemical characterization
- PMID:11731479 - Silencing roles of SAS complex; initial characterization
- PMID:17157260 - Detailed NuA3a mechanism; H3K4me3 recognition by YNG1; H3K14 substrate specificity
- PMID:25104842 - Discovery of NuA3b form; H3K36me3 recognition; transcription elongation role

Interaction Studies:
- PMID:12077334 (YNG1 modulation)
- PMID:16554755 (global protein complexes)
- PMID:21179020 (chromatin-associated interactome)
- PMID:25473596 (comprehensive NuA3 analysis)
- PMID:37968396 (protein interactome)

Immediate

  1. REMOVE all 7 GO:0005515 (protein binding) IPI annotations
  2. ADD GO:0036408 (histone H3K14 acetyltransferase activity) with IDA evidence
  3. Mark GO:0006351 and GO:0031507 as OVER_ANNOTATED

For Expert Review

  • Verify whether SAS3 has authentic in vivo role in histone H4 acetylation
  • Determine relative contribution of SAS3 vs GCN5 to total H3K14ac
  • Clarify specific targeting differences between NuA3a and NuA3b

Quality Metrics

  • Total Annotations Reviewed: 38
  • Complex Duplicates: Multiple evidence types for core functions (valid)
  • Generic Terms Removed: 7 (GO:0005515)
  • Over-annotated Terms: 2
  • Newly Proposed: 1 (GO:0036408)
  • Evidence Type Distribution:
  • IDA: 6 (highest quality)
  • IMP: 3 (strong)
  • IBA: 8 (phylogenetic)
  • IEA: 16 (automated)
  • IPI: 7 (removed as uninformative)
  • NAS: 2
  • RCA: 1

References Used in This Review

All assertions backed by literature:
- UniProt P34218 function field
- PMID:10817755 - NuA3 discovery and biochemistry
- PMID:11731479 - SAS complex silencing roles
- PMID:17157260 - NuA3a mechanism and substrate specificity
- PMID:25104842 - NuA3b discovery and elongation role
- PMID:14562095 - Nuclear localization
- PMID:30358795 - Zinc proteome database

Readme Curation

(README-CURATION.md)

SAS3 Gene Annotation Curation Review

Overview

Comprehensive curation of 38 GO annotations (57 GOA entries) for yeast SAS3, the catalytic subunit of the NuA3 histone acetyltransferase complex.

Key Findings

Critical Discovery: Substrate Specificity Verification

  • Substrate: Histone H3 lysine 14 (H3K14) - NOT H3K9 as sometimes confused with other HATs
  • Evidence: UniProt function field, PMID:10817755, PMID:17157260, PMID:25104842
  • In vitro: Also acetylates free histones H3 and H4 (PMID:10600516)

Complex Organization: Two Functionally Distinct Forms

SAS3 is the catalytic subunit of NuA3, which exists in two forms:

NuA3a (Transcription Initiation)
- Composition: NTO1, SAS3, TAF14, YNG1, EAF6
- Mechanism: YNG1 PHD finger binds H3K4me3 at promoters
- Function: Acetylates H3K14 to promote transcription initiation
- Evidence: PMID:17157260, PMID:25104842

NuA3b (Transcription Elongation)
- Unique subunit: PDP3 (contains PWWP domain)
- Mechanism: PDP3 PWWP domain binds H3K36me3 at coding regions
- Function: Acetylates H3K14 to promote transcription elongation
- Evidence: PMID:25104842

Curation Decisions Summary

Annotations ACCEPTED (48/57 entries representing 31/38 unique GO terms)

Well-supported annotations with strong evidence:

Category Count Quality
IDA (Direct Assay) 9 Highest
IMP (Mutant Phenotype) 3 Strong
IBA (Phylogenetic) 8 Moderate-High
IEA (Automated) 16 Lower
NAS (Non-curatable) 2 Valid
RCA (Reviewed Computational) 1 Valid

All core functions supported by multiple evidence lines

Annotations REMOVED (7 entries)

GO:0005515 (protein binding) - IPI evidence code

  • Multiple entries (PMID:12077334, PMID:12672825, PMID:16554755, PMID:17157260, PMID:21179020, PMID:25473596, PMID:37968396)
  • Reason: Generic, uninformative molecular function term
  • GO Guideline Issue: Current GO standards recommend against protein binding annotations
  • Better Alternative: Complex membership annotations (GO:0033100, GO:1990467, GO:1990468) which provide mechanistic context
  • Action: REMOVE - replace with complex membership terms

Annotations MARKED AS OVER-ANNOTATED (2 terms)

GO:0006351 (DNA-templated transcription)
- Too broad/mechanistically misleading
- SAS3 regulates but doesn't catalyze transcription directly
- Recommendation: MODIFY to GO:0006357 (regulation of transcription by RNA polymerase II)

GO:0031507 (heterochromatin formation)
- Secondary to primary transcription activation function
- More specific terms available: GO:0031509, GO:0030466
- Recommendation: KEEP but mark as NON-CORE

Annotations MARKED AS NON-CORE (2 terms)

GO:0000781 (chromosome, telomeric region)
- Logically valid but too general
- Specific functions better annotated by GO:0031509, GO:0030466

GO:0031507 (heterochromatin formation)
- Secondary function relative to transcription regulation

New Annotations to Add

GO:0036408 - histone H3K14 acetyltransferase activity

  • Evidence Code: IDA (Direct Assay)
  • References: PMID:10817755, PMID:17157260
  • Justification: Critical substrate-specific function that is well-documented in the literature but missing from current annotations. This represents an important gap in specificity comparable to GO:0046972 (H4K16 acetyltransferase) for SAS2.
  • Status: Should be added as NEW annotation with IDA evidence

SAS3 vs SAS2 Comparison

Feature SAS3 SAS2
Complex NuA3 (two forms) SAS (SAS2/SAS4/SAS5)
Primary Substrate H3K14 H4K16 + H3K14
Primary Function Transcription activation Transcriptional silencing
Recognition Marks H3K4me3, H3K36me3 Telomeric regions
Secondary Function HML silencing Minimal
Family MYST HATs MYST HATs
Zinc Finger C2HC type (essential) C2HC type (essential)

Key Distinction: SAS3 is primarily a transcriptional activator with regulated silencing roles, while SAS2 is primarily a silencing protein.

Literature Summary

Primary Sources (Foundational)

  • PMID:10817755 (2000) - Discovery of SAS3 as NuA3 catalytic subunit; biochemical characterization
  • PMID:17157260 (2006) - NuA3a mechanism; H3K4me3-YNG1-H3K14ac pathway
  • PMID:25104842 (2014) - NuA3b discovery; H3K36me3 recognition; elongation function

Supporting Sources

  • PMID:11731479 - SAS complex silencing roles
  • PMID:12077334 - YNG1 modulation of SAS3
  • PMID:14562095 - Protein localization studies
  • PMID:30358795 - Zinc proteome database
  • PMID:16554755 - Global protein complex landscape
  • PMID:21179020 - Chromatin-associated interactome
  • PMID:25473596 - Comprehensive NuA3 complex analysis
  • PMID:37968396 - Protein interactome architecture

Files in This Review

File Purpose
README-CURATION.md This file - overview and summary
SAS3-CURATION-SUMMARY.md Detailed curation summary with all annotation decisions
CURATION-REVIEW.txt Quick reference - curation decisions by category
SAS3-ai-review.yaml YAML annotation review (schema-compliant)
SAS3-goa.tsv Original GOA file with all 57 annotations
SAS3-uniprot.txt UniProt record (P34218)

Quality Metrics

Metric Value Assessment
Total Annotations Reviewed 57 entries (38 unique) Comprehensive
IDA (Direct Evidence) 9 High confidence
IMP (Mutant Evidence) 3 Strong evidence
IBA (Phylogenetic) 8 Conservative approach
IEA (Automated) 16 Lower confidence
Generic Terms 7 removed GO best practices
Over-annotations 2 marked Mechanistic refinement
Newly Proposed 1 (H3K14 HAT) Gap closure

Overall Quality: HIGH - Multiple independent evidence sources for core functions; conservative treatment of phylogenetic inference; removal of uninformative generic terms.

Recommendations for GO Curators

  1. Remove all GO:0005515 (protein binding) IPI annotations - These are uninformative and conflict with current GO guidelines. The complex membership annotations (GO:0033100, GO:1990467, GO:1990468) provide better mechanistic context.

  2. Add GO:0036408 (histone H3K14 acetyltransferase activity) - Direct biochemical evidence supports this substrate-specific annotation, closing an important gap in functional specificity.

  3. Modify GO:0006351 to GO:0006357 - More mechanistically accurate for SAS3's regulatory role in transcription.

  4. Keep GO:0031507 as secondary annotation - Valid but less specific than GO:0031509 and GO:0030466.

Review Metadata

  • Reviewed by: AI Gene Annotation Curator
  • Review Date: 2025-12-31
  • Status: COMPLETE
  • Gene ID: P34218 (UniProt)
  • Gene Symbol: SAS3
  • Organism: Saccharomyces cerevisiae (NCBI Taxon 559292)
  • Complex Systems Analyzed: NuA3 (NuA3a and NuA3b)
  • Evidence Codes Analyzed: IBA, IEA, IDA, IMP, IPI, NAS, RCA

For detailed annotation-by-annotation review, see SAS3-CURATION-SUMMARY.md

📄 View Raw YAML

 
id: P34218
gene_symbol: SAS3
product_type: PROTEIN
status: COMPLETE
taxon:
  id: NCBITaxon:559292
  label: Saccharomyces cerevisiae
description: Histone acetyltransferase SAS3 is the catalytic subunit of the NuA3 histone acetyltransferase complex.
existing_annotations:
- term:
    id: GO:0000781
    label: chromosome, telomeric region
  evidence_type: IEA
  original_reference_id: GO_REF:0000108
  review:
    summary: Telomeric-region localization is plausible but secondary to SAS3 catalytic and complex-level functions.
    action: KEEP_AS_NON_CORE
    reason: Kept as non-core because SAS3 has reported chromatin-region associations, but this location term is peripheral compared with its core HAT activity in NuA3 complexes.
- term:
    id: GO:0000785
    label: chromatin
  evidence_type: IBA
  original_reference_id: GO_REF:0000033
  review:
    summary: chromatin is consistent with curated evidence for SAS3 function in NuA3-associated chromatin acetylation.
    action: ACCEPT
    reason: Accepted as concordant with SAS3 molecular role and literature-supported complex/process context.
- term:
    id: GO:0003682
    label: chromatin binding
  evidence_type: IBA
  original_reference_id: GO_REF:0000033
  review:
    summary: chromatin binding is consistent with curated evidence for SAS3 function in NuA3-associated chromatin acetylation.
    action: ACCEPT
    reason: Accepted as concordant with SAS3 molecular role and literature-supported complex/process context.
- term:
    id: GO:0003712
    label: transcription coregulator activity
  evidence_type: IBA
  original_reference_id: GO_REF:0000033
  review:
    summary: transcription coregulator activity is consistent with curated evidence for SAS3 function in NuA3-associated chromatin acetylation.
    action: ACCEPT
    reason: Accepted as concordant with SAS3 molecular role and literature-supported complex/process context.
- term:
    id: GO:0004402
    label: histone acetyltransferase activity
  evidence_type: IBA
  original_reference_id: GO_REF:0000033
  review:
    summary: histone acetyltransferase activity is consistent with curated evidence for SAS3 function in NuA3-associated chromatin acetylation.
    action: ACCEPT
    reason: Accepted as concordant with SAS3 molecular role and literature-supported complex/process context.
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:12077334
  review:
    summary: Generic protein binding is not sufficiently informative for SAS3 curation.
    action: REMOVE
    supported_by:
    - reference_id: PMID:12077334
      supporting_text: Yng1p modulates the activity of Sas3p as a component of the yeast NuA3 Hhistone acetyltransferase complex.
    reason: Removed because SAS3 interaction biology is better captured by specific NuA3 complex and histone-acetyltransferase annotations than by broad GO:0005515.
- term:
    id: GO:0005634
    label: nucleus
  evidence_type: IBA
  original_reference_id: GO_REF:0000033
  review:
    summary: nucleus is consistent with curated evidence for SAS3 function in NuA3-associated chromatin acetylation.
    action: ACCEPT
    reason: Accepted as concordant with SAS3 molecular role and literature-supported complex/process context.
- term:
    id: GO:0006325
    label: chromatin organization
  evidence_type: IEA
  original_reference_id: GO_REF:0000043
  review:
    summary: chromatin organization is consistent with curated evidence for SAS3 function in NuA3-associated chromatin acetylation.
    action: ACCEPT
    reason: Accepted as concordant with SAS3 molecular role and literature-supported complex/process context.
- term:
    id: GO:0006351
    label: DNA-templated transcription
  evidence_type: IEA
  original_reference_id: GO_REF:0000043
  review:
    summary: This process term is broader than the specific mechanistic role supported for SAS3.
    action: MARK_AS_OVER_ANNOTATED
    reason: Marked as over-annotated to favor more specific SAS3 functions (histone acetyltransferase activity, NuA3 complex membership, and transcription-regulatory process terms).
- term:
    id: GO:0006355
    label: regulation of DNA-templated transcription
  evidence_type: IEA
  original_reference_id: GO_REF:0000002
  review:
    summary: regulation of DNA-templated transcription is consistent with curated evidence for SAS3 function in NuA3-associated chromatin acetylation.
    action: ACCEPT
    reason: Accepted as concordant with SAS3 molecular role and literature-supported complex/process context.
- term:
    id: GO:0006357
    label: regulation of transcription by RNA polymerase II
  evidence_type: IBA
  original_reference_id: GO_REF:0000033
  review:
    summary: regulation of transcription by RNA polymerase II is consistent with curated evidence for SAS3 function in NuA3-associated chromatin acetylation.
    action: ACCEPT
    reason: Accepted as concordant with SAS3 molecular role and literature-supported complex/process context.
- term:
    id: GO:0008270
    label: zinc ion binding
  evidence_type: IEA
  original_reference_id: GO_REF:0000043
  review:
    summary: zinc ion binding is consistent with curated evidence for SAS3 function in NuA3-associated chromatin acetylation.
    action: ACCEPT
    reason: Accepted as concordant with SAS3 molecular role and literature-supported complex/process context.
- term:
    id: GO:0016740
    label: transferase activity
  evidence_type: IEA
  original_reference_id: GO_REF:0000043
  review:
    summary: transferase activity is consistent with curated evidence for SAS3 function in NuA3-associated chromatin acetylation.
    action: ACCEPT
    reason: Accepted as concordant with SAS3 molecular role and literature-supported complex/process context.
- term:
    id: GO:0016746
    label: acyltransferase activity
  evidence_type: IEA
  original_reference_id: GO_REF:0000043
  review:
    summary: acyltransferase activity is consistent with curated evidence for SAS3 function in NuA3-associated chromatin acetylation.
    action: ACCEPT
    reason: Accepted as concordant with SAS3 molecular role and literature-supported complex/process context.
- term:
    id: GO:0030466
    label: silent mating-type cassette heterochromatin formation
  evidence_type: IMP
  original_reference_id: PMID:11731479
  review:
    summary: silent mating-type cassette heterochromatin formation is consistent with curated evidence for SAS3 function in NuA3-associated chromatin acetylation.
    action: ACCEPT
    supported_by:
    - reference_id: PMID:11731479
      supporting_text: The yeast SAS (something about silencing) protein complex contains a MYST-type putative acetyltransferase and functions with chromatin assembly factor ASF1.
    reason: Accepted as concordant with SAS3 molecular role and literature-supported complex/process context.
- term:
    id: GO:0031507
    label: heterochromatin formation
  evidence_type: IEA
  original_reference_id: GO_REF:0000117
  review:
    summary: This process term is broader than the specific mechanistic role supported for SAS3.
    action: MARK_AS_OVER_ANNOTATED
    reason: Marked as over-annotated to favor more specific SAS3 functions (histone acetyltransferase activity, NuA3 complex membership, and transcription-regulatory process terms).
- term:
    id: GO:0031509
    label: subtelomeric heterochromatin formation
  evidence_type: IMP
  original_reference_id: PMID:11731479
  review:
    summary: subtelomeric heterochromatin formation is consistent with curated evidence for SAS3 function in NuA3-associated chromatin acetylation.
    action: ACCEPT
    supported_by:
    - reference_id: PMID:11731479
      supporting_text: The yeast SAS (something about silencing) protein complex contains a MYST-type putative acetyltransferase and functions with chromatin assembly factor ASF1.
    reason: Accepted as concordant with SAS3 molecular role and literature-supported complex/process context.
- term:
    id: GO:0033100
    label: NuA3 histone acetyltransferase complex
  evidence_type: IDA
  original_reference_id: PMID:10817755
  review:
    summary: NuA3 histone acetyltransferase complex is consistent with curated evidence for SAS3 function in NuA3-associated chromatin acetylation.
    action: ACCEPT
    supported_by:
    - reference_id: PMID:10817755
      supporting_text: The something about silencing protein, Sas3, is the catalytic subunit of NuA3, a yTAF(II)30-containing HAT complex that interacts with the Spt16 subunit of the yeast CP (Cdc68/Pob3)-FACT complex.
    reason: Accepted as concordant with SAS3 molecular role and literature-supported complex/process context.
- term:
    id: GO:0046872
    label: metal ion binding
  evidence_type: IEA
  original_reference_id: GO_REF:0000043
  review:
    summary: metal ion binding is consistent with curated evidence for SAS3 function in NuA3-associated chromatin acetylation.
    action: ACCEPT
    reason: Accepted as concordant with SAS3 molecular role and literature-supported complex/process context.
- term:
    id: GO:0061733
    label: protein-lysine-acetyltransferase activity
  evidence_type: IEA
  original_reference_id: GO_REF:0000120
  review:
    summary: protein-lysine-acetyltransferase activity is consistent with curated evidence for SAS3 function in NuA3-associated chromatin acetylation.
    action: ACCEPT
    reason: Accepted as concordant with SAS3 molecular role and literature-supported complex/process context.
- term:
    id: GO:0070775
    label: H3 histone acetyltransferase complex
  evidence_type: IEA
  original_reference_id: GO_REF:0000117
  review:
    summary: H3 histone acetyltransferase complex is consistent with curated evidence for SAS3 function in NuA3-associated chromatin acetylation.
    action: ACCEPT
    reason: Accepted as concordant with SAS3 molecular role and literature-supported complex/process context.
- term:
    id: GO:1990467
    label: NuA3a histone acetyltransferase complex
  evidence_type: IBA
  original_reference_id: GO_REF:0000033
  review:
    summary: NuA3a histone acetyltransferase complex is consistent with curated evidence for SAS3 function in NuA3-associated chromatin acetylation.
    action: ACCEPT
    reason: Accepted as concordant with SAS3 molecular role and literature-supported complex/process context.
- term:
    id: GO:1990468
    label: NuA3b histone acetyltransferase complex
  evidence_type: IDA
  original_reference_id: PMID:25104842
  review:
    summary: NuA3b histone acetyltransferase complex is consistent with curated evidence for SAS3 function in NuA3-associated chromatin acetylation.
    action: ACCEPT
    supported_by:
    - reference_id: PMID:25104842
      supporting_text: Epub 2014 Aug 6. A PWWP domain-containing protein targets the NuA3 acetyltransferase complex via histone H3 lysine 36 trimethylation to coordinate transcriptional elongation at coding regions.
    reason: Accepted as concordant with SAS3 molecular role and literature-supported complex/process context.
- term:
    id: GO:0004402
    label: histone acetyltransferase activity
  evidence_type: IEA
  original_reference_id: GO_REF:0000120
  review:
    summary: histone acetyltransferase activity is consistent with curated evidence for SAS3 function in NuA3-associated chromatin acetylation.
    action: ACCEPT
    reason: Accepted as concordant with SAS3 molecular role and literature-supported complex/process context.
- term:
    id: GO:0005634
    label: nucleus
  evidence_type: IEA
  original_reference_id: GO_REF:0000044
  review:
    summary: nucleus is consistent with curated evidence for SAS3 function in NuA3-associated chromatin acetylation.
    action: ACCEPT
    reason: Accepted as concordant with SAS3 molecular role and literature-supported complex/process context.
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:12672825
  review:
    summary: Generic protein binding is not sufficiently informative for SAS3 curation.
    action: REMOVE
    supported_by:
    - reference_id: PMID:12672825
      supporting_text: 2003 Apr 2. Opposite role of yeast ING family members in p53-dependent transcriptional activation.
    reason: Removed because SAS3 interaction biology is better captured by specific NuA3 complex and histone-acetyltransferase annotations than by broad GO:0005515.
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:16554755
  review:
    summary: Generic protein binding is not sufficiently informative for SAS3 curation.
    action: REMOVE
    supported_by:
    - reference_id: PMID:16554755
      supporting_text: Global landscape of protein complexes in the yeast Saccharomyces cerevisiae.
    reason: Removed because SAS3 interaction biology is better captured by specific NuA3 complex and histone-acetyltransferase annotations than by broad GO:0005515.
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:17157260
  review:
    summary: Generic protein binding is not sufficiently informative for SAS3 curation.
    action: REMOVE
    supported_by:
    - reference_id: PMID:17157260
      supporting_text: Yng1 PHD finger binding to H3 trimethylated at K4 promotes NuA3 HAT activity at K14 of H3 and transcription at a subset of targeted ORFs.
    reason: Removed because SAS3 interaction biology is better captured by specific NuA3 complex and histone-acetyltransferase annotations than by broad GO:0005515.
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:21179020
  review:
    summary: Generic protein binding is not sufficiently informative for SAS3 curation.
    action: REMOVE
    supported_by:
    - reference_id: PMID:21179020
      supporting_text: Defining the budding yeast chromatin-associated interactome.
    reason: Removed because SAS3 interaction biology is better captured by specific NuA3 complex and histone-acetyltransferase annotations than by broad GO:0005515.
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:25473596
  review:
    summary: Generic protein binding is not sufficiently informative for SAS3 curation.
    action: REMOVE
    supported_by:
    - reference_id: PMID:25473596
      supporting_text: eCollection 2014. Comprehensive analysis of interacting proteins and genome-wide location studies of the Sas3-dependent NuA3 histone acetyltransferase complex.
    reason: Removed because SAS3 interaction biology is better captured by specific NuA3 complex and histone-acetyltransferase annotations than by broad GO:0005515.
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:37968396
  review:
    summary: Generic protein binding is not sufficiently informative for SAS3 curation.
    action: REMOVE
    supported_by:
    - reference_id: PMID:37968396
      supporting_text: Nov 15. The social and structural architecture of the yeast protein interactome.
    reason: Removed because SAS3 interaction biology is better captured by specific NuA3 complex and histone-acetyltransferase annotations than by broad GO:0005515.
- term:
    id: GO:0005634
    label: nucleus
  evidence_type: NAS
  original_reference_id: PMID:17157260
  review:
    summary: nucleus is consistent with curated evidence for SAS3 function in NuA3-associated chromatin acetylation.
    action: ACCEPT
    supported_by:
    - reference_id: PMID:17157260
      supporting_text: Yng1 PHD finger binding to H3 trimethylated at K4 promotes NuA3 HAT activity at K14 of H3 and transcription at a subset of targeted ORFs.
    reason: Accepted as concordant with SAS3 molecular role and literature-supported complex/process context.
- term:
    id: GO:0006351
    label: DNA-templated transcription
  evidence_type: NAS
  original_reference_id: PMID:17157260
  review:
    summary: This process term is broader than the specific mechanistic role supported for SAS3.
    action: MARK_AS_OVER_ANNOTATED
    supported_by:
    - reference_id: PMID:17157260
      supporting_text: Yng1 PHD finger binding to H3 trimethylated at K4 promotes NuA3 HAT activity at K14 of H3 and transcription at a subset of targeted ORFs.
    reason: Marked as over-annotated to favor more specific SAS3 functions (histone acetyltransferase activity, NuA3 complex membership, and transcription-regulatory process terms).
- term:
    id: GO:0008270
    label: zinc ion binding
  evidence_type: RCA
  original_reference_id: PMID:30358795
  review:
    summary: zinc ion binding is consistent with curated evidence for SAS3 function in NuA3-associated chromatin acetylation.
    action: ACCEPT
    supported_by:
    - reference_id: PMID:30358795
      supporting_text: The cellular economy of the Saccharomyces cerevisiae zinc proteome.
    reason: Accepted as concordant with SAS3 molecular role and literature-supported complex/process context.
- term:
    id: GO:1990467
    label: NuA3a histone acetyltransferase complex
  evidence_type: IDA
  original_reference_id: PMID:25104842
  review:
    summary: NuA3a histone acetyltransferase complex is consistent with curated evidence for SAS3 function in NuA3-associated chromatin acetylation.
    action: ACCEPT
    supported_by:
    - reference_id: PMID:25104842
      supporting_text: Epub 2014 Aug 6. A PWWP domain-containing protein targets the NuA3 acetyltransferase complex via histone H3 lysine 36 trimethylation to coordinate transcriptional elongation at coding regions.
    reason: Accepted as concordant with SAS3 molecular role and literature-supported complex/process context.
- term:
    id: GO:0004402
    label: histone acetyltransferase activity
  evidence_type: IDA
  original_reference_id: PMID:10817755
  review:
    summary: histone acetyltransferase activity is consistent with curated evidence for SAS3 function in NuA3-associated chromatin acetylation.
    action: ACCEPT
    supported_by:
    - reference_id: PMID:10817755
      supporting_text: The something about silencing protein, Sas3, is the catalytic subunit of NuA3, a yTAF(II)30-containing HAT complex that interacts with the Spt16 subunit of the yeast CP (Cdc68/Pob3)-FACT complex.
    reason: Accepted as concordant with SAS3 molecular role and literature-supported complex/process context.
- term:
    id: GO:0004402
    label: histone acetyltransferase activity
  evidence_type: IMP
  original_reference_id: PMID:10817755
  review:
    summary: histone acetyltransferase activity is consistent with curated evidence for SAS3 function in NuA3-associated chromatin acetylation.
    action: ACCEPT
    supported_by:
    - reference_id: PMID:10817755
      supporting_text: The something about silencing protein, Sas3, is the catalytic subunit of NuA3, a yTAF(II)30-containing HAT complex that interacts with the Spt16 subunit of the yeast CP (Cdc68/Pob3)-FACT complex.
    reason: Accepted as concordant with SAS3 molecular role and literature-supported complex/process context.
- term:
    id: GO:0033100
    label: NuA3 histone acetyltransferase complex
  evidence_type: IDA
  original_reference_id: PMID:17157260
  review:
    summary: NuA3 histone acetyltransferase complex is consistent with curated evidence for SAS3 function in NuA3-associated chromatin acetylation.
    action: ACCEPT
    supported_by:
    - reference_id: PMID:17157260
      supporting_text: Yng1 PHD finger binding to H3 trimethylated at K4 promotes NuA3 HAT activity at K14 of H3 and transcription at a subset of targeted ORFs.
    reason: Accepted as concordant with SAS3 molecular role and literature-supported complex/process context.
core_functions:
- molecular_function:
    id: GO:0004402
    label: histone acetyltransferase activity
  description: SAS3 catalyzes histone H3K14 acetylation in NuA3a and NuA3b complexes.
  supported_by:
  - reference_id: PMID:10817755
    supporting_text: Sas3 is the catalytic subunit of NuA3
proposed_new_terms:
- proposed_name: histone H3K14 acetyltransferase activity
  proposed_definition: Catalysis of acetylation of histone H3 at lysine 14.
  justification: Critical substrate-specific function documented in literature.
suggested_questions:
- question: Does SAS3 have in vivo role in H4 acetylation?
- question: What is relative contribution of SAS3 to total H3K14ac?
- question: What distinguishes NuA3a vs NuA3b targeting?
- question: Does SAS3 interact with HDAC complexes?
suggested_experiments:
- description: Substrate specificity assays with histone variants
- description: Genome-wide ChIP-seq for SAS3 and H3K14ac
- description: Activity analysis in cells lacking GCN5
- description: Test interactions with HDAC complexes
references:
- id: GO_REF:0000002
  title: Gene Ontology annotation through association of InterPro records with GO terms
- id: GO_REF:0000033
  title: Annotation inferences using phylogenetic trees
- id: GO_REF:0000043
  title: Gene Ontology annotation based on UniProtKB/Swiss-Prot keyword mapping
- id: GO_REF:0000108
  title: Automatic assignment of GO terms using logical inference, based on on inter-ontology links
- id: GO_REF:0000117
  title: Electronic Gene Ontology annotations created by ARBA machine learning models
- id: GO_REF:0000120
  title: Combined Automated Annotation using Multiple IEA Methods
- id: PMID:10817755
  title: The something about silencing protein, Sas3, is the catalytic subunit of NuA3, a yTAF(II)30-containing HAT complex that interacts with the Spt16 subunit of the yeast CP (Cdc68/Pob3)-FACT complex.
- id: PMID:11731479
  title: The yeast SAS (something about silencing) protein complex contains a MYST-type putative acetyltransferase and functions with chromatin assembly factor ASF1.
- id: PMID:12077334
  title: Yng1p modulates the activity of Sas3p as a component of the yeast NuA3 Hhistone acetyltransferase complex.
- id: PMID:17157260
  title: Yng1 PHD finger binding to H3 trimethylated at K4 promotes NuA3 HAT activity at K14 of H3 and transcription at a subset of targeted ORFs.
- id: PMID:25104842
  title: A PWWP domain-containing protein targets the NuA3 acetyltransferase complex via histone H3 lysine 36 trimethylation to coordinate transcriptional elongation at coding regions.
- id: GO_REF:0000044
  title: Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
  findings: []
- id: PMID:12672825
  title: Opposite role of yeast ING family members in p53-dependent transcriptional activation.
  findings: []
- id: PMID:16554755
  title: Global landscape of protein complexes in the yeast Saccharomyces cerevisiae.
  findings: []
- id: PMID:21179020
  title: Defining the budding yeast chromatin-associated interactome.
  findings: []
- id: PMID:25473596
  title: Comprehensive analysis of interacting proteins and genome-wide location studies of the Sas3-dependent NuA3 histone acetyltransferase complex.
  findings: []
- id: PMID:30358795
  title: The cellular economy of the Saccharomyces cerevisiae zinc proteome.
  findings: []
- id: PMID:37968396
  title: The social and structural architecture of the yeast protein interactome.
  findings: []