Flavoprotein subunit (Sdh1p, YKL148C; SDHA homolog) of mitochondrial succinate dehydrogenase (complex II, succinate:ubiquinone oxidoreductase, EC 1.3.5.1). Sdh1 carries a covalently bound FAD and the dicarboxylate site at which succinate is oxidised to fumarate in the TCA cycle; electrons pass from FAD through the iron-sulfur clusters of Sdh2 to ubiquinone bound by the membrane anchor Sdh3/Sdh4 (with heme b562), linking the TCA cycle to the respiratory chain. Sdh1 is a peripheral subunit on the matrix face of the inner membrane. Its flavination needs the assembly factor Sdh5, the chaperone Sdh8 keeps flavinated Sdh1 competent to bind Sdh2, and Tcm62 assists complex assembly. Loss of Sdh1 abolishes SDH activity and respiratory growth; the weakly expressed paralog SDH1b (YJL045W) can partially substitute.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0005515 protein binding | IPI PMID:16429126 Proteome survey reveals modularity of the yeast cell machine... | REMOVE | Summary: IPI Sdh1-Sdh2 from the Krogan et al. 2006 TAP-MS survey. Sdh1 and Sdh2 form the catalytic dimer of complex II, so the interaction is genuine and removal does not mean it is false; 'protein binding' adds nothing beyond part_of respiratory chain complex II (GO:0045273). Supporting Evidence: PMID:16429126 Proteome survey reveals modularity of the yeast cell machinery. PMID:24954416 The Sdh3/Sdh4 dimer binds the peripheral membrane protein Sdh2 (SDHB), which tethers the catalytic Sdh1 (SDHA) subunit to the complex |
| GO:0005515 protein binding | IPI PMID:19628817 SDH5, a gene required for flavination of succinate dehydroge... | REMOVE | Summary: IPI Sdh1-Sdh5 (UniProtKB:Q08230) from Hao et al. 2009. Sdh5 is the assembly factor required for covalent FAD attachment to Sdh1; the interaction is real, but the informative function lies with Sdh5 (flavination), and 'protein binding' on Sdh1 is uninformative. Removal does not assert the interaction is false. Supporting Evidence: PMID:19628817 Sdh5 is required for SDH-dependent respiration and for Sdh1 flavination PMID:19628817 Both yeast and human Sdh5 interact with the catalytic subunit of the succinate dehydrogenase (SDH) complex |
| GO:0005515 protein binding | IPI PMID:24954416 SDHAF4 promotes mitochondrial succinate dehydrogenase activi... | REMOVE | Summary: IPI Sdh1-Sdh2 from Van Vranken et al. 2014. Sdh1 and Sdh2 form the catalytic dimer of complex II, so the interaction is genuine and removal does not mean it is false; 'protein binding' adds nothing beyond part_of respiratory chain complex II (GO:0045273). Supporting Evidence: PMID:24954416 Sdh8 interacts specifically with the catalytic Sdh1 subunit in the mitochondrial matrix, facilitating its association with Sdh2 and the subsequent assembly of the SDH holocomplex PMID:24954416 The Sdh3/Sdh4 dimer binds the peripheral membrane protein Sdh2 (SDHB), which tethers the catalytic Sdh1 (SDHA) subunit to the complex |
| GO:0005515 protein binding | IPI PMID:24954416 SDHAF4 promotes mitochondrial succinate dehydrogenase activi... | REMOVE | Summary: IPI Sdh1-Sdh8 (UniProtKB:P38345, SDHAF4) from Van Vranken et al. 2014. Sdh8 is a matrix chaperone/assembly factor for flavinated Sdh1; the interaction is real but the function belongs to Sdh8, and 'protein binding' on Sdh1 is uninformative. Removal does not assert the interaction is false. Supporting Evidence: PMID:24954416 Sdh8 interacts specifically with the catalytic Sdh1 subunit in the mitochondrial matrix, facilitating its association with Sdh2 and the subsequent assembly of the SDH holocomplex |
| GO:0005515 protein binding | IPI PMID:37968396 The social and structural architecture of the yeast protein ... | REMOVE | Summary: IPI Sdh1-Sdh2 from the Michaelis et al. 2023 interactome. Sdh1 and Sdh2 form the catalytic dimer of complex II, so the interaction is genuine and removal does not mean it is false; 'protein binding' adds nothing beyond part_of respiratory chain complex II (GO:0045273). Supporting Evidence: PMID:37968396 The social and structural architecture of the yeast protein interactome. PMID:24954416 The Sdh3/Sdh4 dimer binds the peripheral membrane protein Sdh2 (SDHB), which tethers the catalytic Sdh1 (SDHA) subunit to the complex |
| GO:0005739 mitochondrion | HDA PMID:16823961 Toward the complete yeast mitochondrial proteome: multidimen... | ACCEPT | Summary: HDA from a mitochondrial proteomics study detecting Sdh1. Correct. Supporting Evidence: PMID:16823961 Toward the complete yeast mitochondrial proteome: multidimensional separation techniques for mitochondrial proteomics. |
| GO:0005739 mitochondrion | HDA PMID:24769239 Quantitative variations of the mitochondrial proteome and ph... | ACCEPT | Summary: HDA from a mitochondrial proteomics study (fermentative vs respiratory growth) detecting Sdh1. Correct. Supporting Evidence: PMID:24769239 Quantitative variations of the mitochondrial proteome and phosphoproteome during fermentative and respiratory growth in Saccharomyces cerevisiae. |
| GO:0005739 mitochondrion | IBA GO_REF:0000033 | ACCEPT | Summary: IBA from PANTHER PTN000180258 (SDHA clade; donors include human SDHA, mouse, rat, Arabidopsis and yeast Sdh1). Correct and inherited. Supporting Evidence: file:yeast/SDH1/SDH1-uniprot.txt SUBCELLULAR LOCATION: Mitochondrion inner membrane |
| GO:0005739 mitochondrion | IDA PMID:2826438 Direct interaction between yeast NADH-ubiquinone oxidoreduct... | ACCEPT | Summary: IDA from Zhu & Beattie 1988: succinate-dependent quinone reduction measured in isolated yeast mitochondria. Abstract-only; deferring to curator. Correct location. Supporting Evidence: PMID:2826438 The reduction of these small quinones by succinate also occurred in wild type mitochondria |
| GO:0005743 mitochondrial inner membrane | IDA PMID:9929002 The Saccharomyces cerevisiae succinate-ubiquinone reductase ... | ACCEPT | Summary: IDA from Oyedotun & Lemire 1999 characterising the membrane-bound yeast SDH tetramer (Sdh1-4) with its cytochrome b562. Correct; Sdh1 is a peripheral, matrix-facing subunit of the inner-membrane complex. Supporting Evidence: PMID:9929002 The Saccharomyces cerevisiae succinate-ubiquinone reductase or succinate dehydrogenase (SDH) is a tetramer of non-equivalent subunits encoded by the SDH1, SDH2, SDH3, and SDH4 genes |
| GO:0005743 mitochondrial inner membrane | IEA GO_REF:0000044 | ACCEPT | Summary: IEA from UniProt subcellular location (SL-0168, Mitochondrion inner membrane). Correct. Supporting Evidence: file:yeast/SDH1/SDH1-uniprot.txt SUBCELLULAR LOCATION: Mitochondrion inner membrane |
| GO:0005743 mitochondrial inner membrane | RCA GO_REF:0000123 | ACCEPT | Summary: RCA from YeastPathways TCA-EUK-PWY placing succinate:ubiquinone oxidoreduction at the inner membrane. Correct. Supporting Evidence: file:yeast/SDH1/SDH1-uniprot.txt SUBCELLULAR LOCATION: Mitochondrion inner membrane |
| GO:0006099 tricarboxylic acid cycle | IDA PMID:9929002 The Saccharomyces cerevisiae succinate-ubiquinone reductase ... | ACCEPT | Summary: IDA from Oyedotun & Lemire 1999 on the purified succinate-ubiquinone reductase; SDH is the TCA-cycle enzyme oxidising succinate to fumarate. Supporting Evidence: PMID:9929002 The Saccharomyces cerevisiae succinate-ubiquinone reductase or succinate dehydrogenase (SDH) is a tetramer of non-equivalent subunits encoded by the SDH1, SDH2, SDH3, and SDH4 genes |
| GO:0006099 tricarboxylic acid cycle | IEA GO_REF:0000120 | ACCEPT | Summary: IEA from InterPro IPR011281 / UniPathway UPA00223. Correct. Supporting Evidence: file:yeast/SDH1/SDH1-uniprot.txt Reaction=a quinone + succinate = fumarate + a quinol; |
| GO:0006121 mitochondrial electron transport, succinate to ubiquinone | IBA GO_REF:0000033 | ACCEPT | Summary: IBA from PANTHER PTN000180258 (SDHA clade, yeast Sdh1 among donors). Correct; complex II passes electrons from succinate to ubiquinone. Supporting Evidence: file:yeast/SDH1/SDH1-uniprot.txt Catalytic subunit of succinate dehydrogenase (SDH) that is |
| GO:0006121 mitochondrial electron transport, succinate to ubiquinone | IDA PMID:2826438 Direct interaction between yeast NADH-ubiquinone oxidoreduct... | ACCEPT | Summary: IDA from Zhu & Beattie 1988: succinate-driven reduction of exogenous quinones in yeast mitochondria. Correct. Supporting Evidence: PMID:2826438 The reduction of these small quinones by succinate also occurred in wild type mitochondria |
| GO:0006121 mitochondrial electron transport, succinate to ubiquinone | IDA PMID:9929002 The Saccharomyces cerevisiae succinate-ubiquinone reductase ... | ACCEPT | Summary: IDA from Oyedotun & Lemire 1999 on the succinate-ubiquinone reductase complex. Correct. Supporting Evidence: PMID:9929002 The Saccharomyces cerevisiae succinate-ubiquinone reductase or succinate dehydrogenase (SDH) is a tetramer of non-equivalent subunits encoded by the SDH1, SDH2, SDH3, and SDH4 genes |
| GO:0008177 succinate dehydrogenase (quinone) activity | IDA PMID:13445213 Studies on succinic dehydrogenase. V. Isolation and properti... | ACCEPT | Summary: IDA from Singer et al. 1957, isolation and characterisation of succinate dehydrogenase from baker's yeast (abstract not cached; deferring to curator). Consistent with all genetic data. Supporting Evidence: PMID:13445213 Studies on succinic dehydrogenase. V. Isolation and properties of the dehydrogenase from baker's yeast. |
| GO:0008177 succinate dehydrogenase (quinone) activity | IEA GO_REF:0000120 | ACCEPT | Summary: IEA from Rhea RHEA:40523 / EC 1.3.5.1 / InterPro IPR011281 / ARBA. Correct. Supporting Evidence: file:yeast/SDH1/SDH1-uniprot.txt Reaction=a quinone + succinate = fumarate + a quinol; |
| GO:0008177 succinate dehydrogenase (quinone) activity | IGI PMID:16232921 Effect of gene disruption of succinate dehydrogenase on succ... | ACCEPT | Summary: IGI from Kubo et al. 2005: SDH activity is lost only in double disruptants of SDH1 with SDH2 or with its paralog SDH1b, showing Sdh1 provides the activity redundantly with Sdh1b in this sake strain. Supporting Evidence: PMID:16232921 the activity disappeared in double disruptants of the SDH1 and SDH2 or SDH1b (the SDH1 homologue) genes |
| GO:0008177 succinate dehydrogenase (quinone) activity | IGI PMID:16232921 Effect of gene disruption of succinate dehydrogenase on succ... | ACCEPT | Summary: IGI from Kubo et al. 2005 (second genetic partner, SDH2 or SDH1b): double disruption abolishes SDH activity. Correct. Supporting Evidence: PMID:16232921 the activity disappeared in double disruptants of the SDH1 and SDH2 or SDH1b (the SDH1 homologue) genes |
| GO:0008177 succinate dehydrogenase (quinone) activity | IMP PMID:16232921 Effect of gene disruption of succinate dehydrogenase on succ... | ACCEPT | Summary: IMP from Kubo et al. 2005: SDH1 disruption reduces SDH activity. Correct. Supporting Evidence: PMID:16232921 the activity disappeared in double disruptants of the SDH1 and SDH2 or SDH1b (the SDH1 homologue) genes |
| GO:0008177 succinate dehydrogenase (quinone) activity | IMP PMID:197391 Isolation and characterization of yeast mutants defective in... | ACCEPT | Summary: IMP from Ciriacy 1977: sdh1 mutants isolated as defective in succinate dehydrogenase (abstract-only). Correct. Supporting Evidence: PMID:197391 Homozygous mdh and sdh1 diploids are unable to sporulate |
| GO:0008177 succinate dehydrogenase (quinone) activity | IMP PMID:9730279 Suppression of sdh1 mutations by the SDH1b gene of Saccharom... | ACCEPT | Summary: IMP from Colby et al. 1998: the sdh1 mutant E264/U2 has no detectable mitochondrial SDH activity and is complemented by SDH1. Supporting Evidence: PMID:9730279 One of the plasmids (pG52/T3) contained SDH1 coding for the flavoprotein subunit of mitochondrial succinate dehydrogenase |
| GO:0008177 succinate dehydrogenase (quinone) activity | ISS GO_REF:0000024 | ACCEPT | Summary: ISS from human SDHA (UniProtKB:P31040). Correct; SDHA is the orthologous flavoprotein subunit. Supporting Evidence: file:yeast/SDH1/SDH1-uniprot.txt Reaction=a quinone + succinate = fumarate + a quinol; |
| GO:0008177 succinate dehydrogenase (quinone) activity | RCA GO_REF:0000123 | ACCEPT | Summary: RCA from YeastPathways TCA-EUK-PWY with contributes_to (succinate + ubiquinone -> fumarate + ubiquinol at the inner membrane). Correct; the quinone-reducing activity requires the full complex. Supporting Evidence: file:yeast/SDH1/SDH1-uniprot.txt Reaction=a quinone + succinate = fumarate + a quinol; PMID:8120006 Sdh4p contributes both to the membrane attachment of the catalytic flavoprotein and iron-sulfur subunits and to electron transfer to ubiquinone |
| GO:0009060 aerobic respiration | RCA GO_REF:0000123 | ACCEPT | Summary: RCA from YeastPathways TCA-EUK-PWY. Correct; SDH links the TCA cycle to the respiratory chain. Supporting Evidence: PMID:16232921 Two double disruptants showed no growth on a medium containing glycerol as the sole carbon source |
| GO:0016491 oxidoreductase activity | IEA GO_REF:0000002 | MODIFY | Summary: IEA from InterPro (FAD-dependent oxidoreductase / FRD-SDH flavoprotein signatures) to the very general 'oxidoreductase activity'. Correct but uninformative. Proposed replacements: succinate dehydrogenase (quinone) activity Supporting Evidence: file:yeast/SDH1/SDH1-uniprot.txt Reaction=a quinone + succinate = fumarate + a quinol; |
| GO:0016627 oxidoreductase activity, acting on the CH-CH group of donors | IEA GO_REF:0000002 | MODIFY | Summary: IEA from InterPro IPR014006 (succinate dehydrogenase/fumarate reductase flavoprotein) to 'oxidoreductase activity, acting on the CH-CH group of donors'. Correct parent; the specific activity is succinate dehydrogenase (quinone). Proposed replacements: succinate dehydrogenase (quinone) activity Supporting Evidence: file:yeast/SDH1/SDH1-uniprot.txt Reaction=a quinone + succinate = fumarate + a quinol; |
| GO:0022900 electron transport chain | IEA GO_REF:0000002 | MODIFY | Summary: IEA from InterPro IPR014006 to 'electron transport chain'. Correct but general; the specific process is mitochondrial electron transport from succinate to ubiquinone. Proposed replacements: mitochondrial electron transport, succinate to ubiquinone Supporting Evidence: file:yeast/SDH1/SDH1-uniprot.txt Catalytic subunit of succinate dehydrogenase (SDH) that is |
| GO:0031966 mitochondrial membrane | IDA PMID:1939170 Isolation and characterization of a Saccharomyces cerevisiae... | TOO GENERAL | Summary: IDA from Robinson et al. 1991: sdh1 disruption removes the flavoprotein and iron-sulfur subunits from mitochondrial membranes. Correct, though less specific than inner membrane. Supporting Evidence: PMID:1939170 Disruption of the flavoprotein subunit gene results in the simultaneous loss of both the iron-sulfur and the flavoprotein subunits from mitochondrial membranes |
| GO:0045273 respiratory chain complex II (succinate dehydrogenase) | IBA GO_REF:0000033 | ACCEPT | Summary: IBA from PANTHER PTN000180258 (SDHA clade). Correct. Supporting Evidence: PMID:9929002 The Saccharomyces cerevisiae succinate-ubiquinone reductase or succinate dehydrogenase (SDH) is a tetramer of non-equivalent subunits encoded by the SDH1, SDH2, SDH3, and SDH4 genes |
| GO:0045273 respiratory chain complex II (succinate dehydrogenase) | IDA PMID:9822678 The Saccharomyces cerevisiae TCM62 gene encodes a chaperone ... | ACCEPT | Summary: IDA from Dibrov et al. 1998: SDH holoenzyme subunits including Sdh1 analysed in the TCM62 assembly study; the extrinsic domain consists of Sdh1 and Sdh2. Supporting Evidence: PMID:9822678 The membrane extrinsic domain, consisting of Sdh1p and Sdh2p, contains a covalent FAD cofactor and three iron-sulfur clusters PMID:9822678 Tcm62p forms a complex containing at least three SDH subunits |
| GO:0045273 respiratory chain complex II (succinate dehydrogenase) | IMP PMID:24954416 SDHAF4 promotes mitochondrial succinate dehydrogenase activi... | ACCEPT | Summary: IMP from Van Vranken et al. 2014: Sdh1 association with Sdh2 and holocomplex assembly analysed in mutants. Supporting Evidence: PMID:24954416 Sdh8 interacts specifically with the catalytic Sdh1 subunit in the mitochondrial matrix, facilitating its association with Sdh2 and the subsequent assembly of the SDH holocomplex PMID:24954416 The Sdh3/Sdh4 dimer binds the peripheral membrane protein Sdh2 (SDHB), which tethers the catalytic Sdh1 (SDHA) subunit to the complex |
| GO:0045273 respiratory chain complex II (succinate dehydrogenase) | IPI PMID:8120006 Isolation and characterization of the Saccharomyces cerevisi... | ACCEPT | Summary: IPI from Bullis & Lemire 1994: SDH purified by blue native electrophoresis contains the flavoprotein, iron-sulfur and two membrane subunits. Supporting Evidence: PMID:8120006 We purified succinate dehydrogenase by blue native gel electrophoresis PMID:8120006 Sdh4p contributes both to the membrane attachment of the catalytic flavoprotein and iron-sulfur subunits and to electron transfer to ubiquinone |
| GO:0045333 cellular respiration | IEA GO_REF:0000117 | TOO GENERAL | Summary: IEA from ARBA00026750. Correct but general. Supporting Evidence: file:yeast/SDH1/SDH1-uniprot.txt Catalytic subunit of succinate dehydrogenase (SDH) that is |
| GO:0045333 cellular respiration | IGI PMID:16232921 Effect of gene disruption of succinate dehydrogenase on succ... | TOO GENERAL | Summary: IGI from Kubo et al. 2005: double disruptants of SDH1 with SDH2 or SDH1b fail to grow on glycerol. Supporting Evidence: PMID:16232921 Two double disruptants showed no growth on a medium containing glycerol as the sole carbon source |
| GO:0045333 cellular respiration | IGI PMID:16232921 Effect of gene disruption of succinate dehydrogenase on succ... | TOO GENERAL | Summary: IGI from Kubo et al. 2005 (second genetic partner). Correct. Supporting Evidence: PMID:16232921 Two double disruptants showed no growth on a medium containing glycerol as the sole carbon source |
| GO:0045333 cellular respiration | IGI PMID:9730279 Suppression of sdh1 mutations by the SDH1b gene of Saccharom... | TOO GENERAL | Summary: IGI from Colby et al. 1998: SDH1b on a plasmid restores respiration of the sdh1 mutant. Supporting Evidence: PMID:9730279 Subclones containing the SDH1 homologue (SDH1b), restored respiration in E264/U2 |
| GO:0045333 cellular respiration | IMP PMID:16232921 Effect of gene disruption of succinate dehydrogenase on succ... | TOO GENERAL | Summary: IMP from Kubo et al. 2005. Correct; respiratory growth depends on SDH. Supporting Evidence: PMID:16232921 Two double disruptants showed no growth on a medium containing glycerol as the sole carbon source |
| GO:0045333 cellular respiration | IMP PMID:9730279 Suppression of sdh1 mutations by the SDH1b gene of Saccharom... | TOO GENERAL | Summary: IMP from Colby et al. 1998: the sdh1 mutant cannot grow on non-fermentable substrates. Supporting Evidence: PMID:9730279 One of the plasmids (pG52/T3) contained SDH1 coding for the flavoprotein subunit of mitochondrial succinate dehydrogenase |
| GO:0050660 flavin adenine dinucleotide binding | IDA PMID:18279395 Succinate dehydrogenase flavoprotein subunit expression in S... | KEEP AS NON CORE | Summary: IDA from Giancaspero et al. 2008 on the FAD-binding flavoprotein Sdh1 and its dependence on the FAD transporter Flx1. Sdh1 carries covalent FAD; cofactor binding is integral to, but subsidiary to, its dehydrogenase activity. Supporting Evidence: PMID:18279395 maintaining the normal activity of mitochondrial FAD-binding enzymes, including lipoamide dehydrogenase and succinate dehydrogenase flavoprotein subunit Sdh1p |
| GO:0050660 flavin adenine dinucleotide binding | IEA GO_REF:0000002 | KEEP AS NON CORE | Summary: IEA from InterPro IPR011281/IPR014006. Correct; covalent FAD is the Sdh1 cofactor. Supporting Evidence: PMID:24954416 Sdh1 harbors a covalently bound FAD cofactor that is required for the oxidation of succinate |
| GO:0050660 flavin adenine dinucleotide binding | IMP PMID:1939170 Isolation and characterization of a Saccharomyces cerevisiae... | KEEP AS NON CORE | Summary: IMP from Robinson et al. 1991: sdh1 disruption strongly reduces covalently attached FAD in mitochondria. Supporting Evidence: PMID:1939170 the mutant contains significantly lower levels of covalently attached flavin adenine dinucleotide cofactor than does the wild type |
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Download this section (compressed HTML)Flavoprotein subunit of succinate dehydrogenase; couples the oxidation of succinate to the transfer of electrons to ubiquinone as part of the TCA cycle and the mitochondrial respiratory chain; FAD binding to Sdh1p is required for the assembly of the succinate dehydrogenase subunits; mutations in human ortholog SDHA are associated with Leigh syndrome and predisposition to several cancers
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