| Aspect | Key points | Evidence (with citation IDs) | Publication (author, year, journal) | URL | Publication date/month if available |
|---|---|---|---|---|---|
| Identity / complex membership | Budding-yeast Snf5 is a core, non-ATPase subunit of the SWI/SNF ATP-dependent chromatin-remodeling complex; it is evolutionarily conserved with human SMARCB1/INI1 and contributes to complex integrity and function. | Snf5 is identified as a core SWI/SNF component in *S. cerevisiae* and homologous to SMARCB1/INI1; SWI/SNF is a large multi-subunit chromatin remodeler. (pqac-00000000, pqac-00000003, pqac-00000004, pqac-00000006) | Kuwahara et al., 2023, *Cancer Medicine*; Lampersberger, 2023, Dissertation | https://doi.org/10.1002/cam4.6255 ; https://doi.org/10.17863/cam.93003 | Jun 2023; Jan 2023 |
| Mechanistic role | Snf5 mainly forms the nucleosome-binding lobe (NBL) of SWI/SNF and acts as a structural/regulatory subunit that helps couple nucleosome recognition to remodeling rather than serving as the ATPase. | Reviews describe Snf5 as the principal component of the NBL and a structural subunit within SWI/SNF family remodelers. (pqac-00000007, pqac-00000020, pqac-00000022) | Chen et al., 2023, *Nucleus*; Eustermann et al., 2024, *Nature Reviews Molecular Cell Biology* | https://doi.org/10.1080/19491034.2023.2165604 ; https://doi.org/10.1038/s41580-023-00683-y | Jan 2023; Dec 2024 |
| Nucleosome interaction | Snf5 engages the nucleosomal H2A-H2B acidic patch through its C-terminal finger helix; Arg669 is the canonical arginine anchor. This acidic-patch contact cooperates with the Snf2 motor and SnAc domain to support ATP-coupled nucleosome sliding/ejection. | Snf5 is annotated as a proximal acidic-patch binder; the finger helix packs against the acidic patch, and finger-helix mutations reduce remodeling in vitro and cell fitness in vivo. (pqac-00000001, pqac-00000007, pqac-00000020) | Eustermann et al., 2024, *Nature Reviews Molecular Cell Biology*; Chen et al., 2023, *Nucleus* | https://doi.org/10.1038/s41580-023-00683-y ; https://doi.org/10.1080/19491034.2023.2165604 | Dec 2024; Jan 2023 |
| Transcription regulation | Snf5 contributes to SWI/SNF-mediated transcriptional control by interacting with activator domains and participating in promoter chromatin remodeling. Recent yeast studies show Swi/Snf can directly repress as well as activate transcription, including via LUTI-based transcriptional interference and metabolic control. | Snf5 binds activator TADs such as Ino2/Gcn4; SWI/SNF mediates direct repression through co-transcriptional downstream remodeling and regulates sulfur metabolic genes. (pqac-00000005, pqac-00000012, pqac-00000013, pqac-00000016, pqac-00000019) | Wendegatz et al., 2024, *Current Genetics*; Morse et al., 2024, *Molecular Cell*; Church et al., 2023, *Nucleic Acids Research* | https://doi.org/10.1007/s00294-024-01300-x ; https://doi.org/10.1016/j.molcel.2024.06.029 ; https://doi.org/10.1093/nar/gkad711 | Sep 2024; Aug 2024; Aug 2023 |
| Quantitative findings | Recent quantified SWI/SNF effects in yeast include: 250 defined Swi/Snf target genes in Morse et al.; average 1.9-fold increase in NDR occupancy at the proximal promoter during HNT1 LUTI repression (p=0.0445); HNT1LUTI induced within 5 min and ~3-fold by 30 min; all 11 validated LUTI-escape mutations mapped to Swi/Snf genes. Church et al. found RNA-seq pathway enrichment of sulfur/MET genes in both snf2Δ and snf5Δ mutants. | Quantitative values and gene-set sizes come from recent genomic and reporter-based yeast studies on Swi/Snf-dependent repression/activation. (pqac-00000008, pqac-00000012, pqac-00000013, pqac-00000016) | Church et al., 2023, *Nucleic Acids Research*; Morse et al., 2024, *Molecular Cell* | https://doi.org/10.1093/nar/gkad711 ; https://doi.org/10.1016/j.molcel.2024.06.029 | Aug 2023; Aug 2024 |
| Applications / implications | Yeast Snf5 provides a mechanistic model for the conserved SNF5/SMARCB1 class in eukaryotes. Its acidic-patch engagement and scaffold-like role inform interpretation of disease-linked mammalian orthologs and broader chromatin-remodeler targeting strategies; in yeast, SWI/SNF studies also inform metabolic engineering and stress-response transcription models. | Expert reviews use yeast Snf5/SMARCB1 conservation to explain SWI/SNF mechanism and disease relevance; recent metabolism-focused work highlights SWI/SNF as a regulator of metabolic transcription. (pqac-00000000, pqac-00000002, pqac-00000020, pqac-00000022) | Kuwahara et al., 2023, *Cancer Medicine*; Church & Workman, 2024, *Biochemical Society Transactions*; Eustermann et al., 2024, *Nature Reviews Molecular Cell Biology* | https://doi.org/10.1002/cam4.6255 ; https://doi.org/10.1042/bst20231141 ; https://doi.org/10.1038/s41580-023-00683-y | Jun 2023; Apr 2024; Dec 2024 |


*Table: This table compiles the main evidence retrieved for Saccharomyces cerevisiae Snf5, focusing on identity, mechanism, nucleosome interactions, transcriptional roles, quantitative findings, and broader implications. It is useful as a concise source map for building a full research report on UniProt P18480.*