VPS45

UniProt ID: P38932
Organism: Saccharomyces cerevisiae
Review Status: DRAFT
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Gene Description

VPS45 encodes a Sec1/Munc18 (SM) family protein essential for vacuolar protein sorting and membrane traffic between the late Golgi and the prevacuolar compartment. Vps45p binds and regulates the SNARE proteins Pep12p (a prevacuolar t-SNARE) and Tlg2p (a late Golgi/early endosome t-SNARE), promoting their proper function in SNARE complex assembly and membrane fusion. It is required for fusion of Golgi-derived transport vesicles with the prevacuolar compartment and also contributes to vacuole inheritance. Mutations in human VPS45 cause severe congenital neutropenia, underscoring the conserved importance of this trafficking regulator. Orthologous to human VPS45.

Existing Annotations Review

GO Term Evidence Action Reason
GO:0016192 vesicle-mediated transport
IBA
GO_REF:0000033
ACCEPT
Summary: Manual review: vesicle-mediated transport is consistent with known biology of VPS45.
Reason: Retained as supported or plausible for this gene and evidence context.
GO:0000139 Golgi membrane
IBA
GO_REF:0000033
ACCEPT
Summary: Manual review: Golgi membrane is consistent with known biology of VPS45.
Reason: Retained as supported or plausible for this gene and evidence context.
GO:0006886 intracellular protein transport
IBA
GO_REF:0000033
ACCEPT
Summary: Manual review: intracellular protein transport is consistent with known biology of VPS45.
Reason: Retained as supported or plausible for this gene and evidence context.
GO:0005737 cytoplasm
IEA
GO_REF:0000044
ACCEPT
Summary: Manual review: cytoplasm is consistent with known biology of VPS45.
Reason: Retained as supported or plausible for this gene and evidence context.
GO:0005774 vacuolar membrane
IEA
GO_REF:0000044
ACCEPT
Summary: Manual review: vacuolar membrane is consistent with known biology of VPS45.
Reason: Retained as supported or plausible for this gene and evidence context.
GO:0005794 Golgi apparatus
IEA
GO_REF:0000117
ACCEPT
Summary: Manual review: Golgi apparatus is consistent with known biology of VPS45.
Reason: Retained as supported or plausible for this gene and evidence context.
GO:0006886 intracellular protein transport
IEA
GO_REF:0000117
ACCEPT
Summary: Manual review: intracellular protein transport is consistent with known biology of VPS45.
Reason: Retained as supported or plausible for this gene and evidence context.
GO:0015031 protein transport
IEA
GO_REF:0000043
ACCEPT
Summary: Manual review: protein transport is consistent with known biology of VPS45.
Reason: Retained as supported or plausible for this gene and evidence context.
GO:0016192 vesicle-mediated transport
IEA
GO_REF:0000120
ACCEPT
Summary: Manual review: vesicle-mediated transport is consistent with known biology of VPS45.
Reason: Retained as supported or plausible for this gene and evidence context.
GO:0031201 SNARE complex
IEA
GO_REF:0000117
ACCEPT
Summary: Manual review: SNARE complex is consistent with known biology of VPS45.
Reason: Retained as supported or plausible for this gene and evidence context.
GO:0031338 regulation of vesicle fusion
IEA
GO_REF:0000117
ACCEPT
Summary: Manual review: regulation of vesicle fusion is consistent with known biology of VPS45.
Reason: Retained as supported or plausible for this gene and evidence context.
GO:0098588 bounding membrane of organelle
IEA
GO_REF:0000117
ACCEPT
Summary: Manual review: bounding membrane of organelle is consistent with known biology of VPS45.
Reason: Retained as supported or plausible for this gene and evidence context.
GO:0005515 protein binding
IPI
PMID:10397773
A role for Tlg1p in the transport of proteins within the Gol...
MARK AS OVER ANNOTATED
Summary: Manual review: protein binding is too generic or over-extended for VPS45.
Reason: Marked over-annotated because more specific terms capture the biology more accurately.
GO:0005515 protein binding
IPI
PMID:10978279
Pep3p/Pep5p complex: a putative docking factor at multiple s...
MARK AS OVER ANNOTATED
Summary: Manual review: protein binding is too generic or over-extended for VPS45.
Reason: Marked over-annotated because more specific terms capture the biology more accurately.
GO:0005515 protein binding
IPI
PMID:12553664
A novel phospholipid-binding protein from the yeast Saccharo...
MARK AS OVER ANNOTATED
Summary: Manual review: protein binding is too generic or over-extended for VPS45.
Reason: Marked over-annotated because more specific terms capture the biology more accurately.
GO:0005515 protein binding
IPI
PMID:16429126
Proteome survey reveals modularity of the yeast cell machine...
MARK AS OVER ANNOTATED
Summary: Manual review: protein binding is too generic or over-extended for VPS45.
Reason: Marked over-annotated because more specific terms capture the biology more accurately.
GO:0005515 protein binding
IPI
PMID:18719252
High-quality binary protein interaction map of the yeast int...
MARK AS OVER ANNOTATED
Summary: Manual review: protein binding is too generic or over-extended for VPS45.
Reason: Marked over-annotated because more specific terms capture the biology more accurately.
GO:0005515 protein binding
IPI
PMID:19667197
The N-terminal peptide of the syntaxin Tlg2p modulates bindi...
MARK AS OVER ANNOTATED
Summary: Manual review: protein binding is too generic or over-extended for VPS45.
Reason: Marked over-annotated because more specific terms capture the biology more accurately.
GO:0005515 protein binding
IPI
PMID:27107014
An inter-species protein-protein interaction network across ...
MARK AS OVER ANNOTATED
Summary: Manual review: protein binding is too generic or over-extended for VPS45.
Reason: Marked over-annotated because more specific terms capture the biology more accurately.
GO:0005515 protein binding
IPI
PMID:37968396
The social and structural architecture of the yeast protein ...
MARK AS OVER ANNOTATED
Summary: Manual review: protein binding is too generic or over-extended for VPS45.
Reason: Marked over-annotated because more specific terms capture the biology more accurately.
GO:0000011 vacuole inheritance
IMP
PMID:1493335
Morphological classification of the yeast vacuolar protein s...
ACCEPT
Summary: Manual review: vacuole inheritance is consistent with known biology of VPS45.
Reason: Retained as supported or plausible for this gene and evidence context.
GO:0000139 Golgi membrane
IDA
PMID:7720726
Yeast Vps45p is a Sec1p-like protein required for the consum...
ACCEPT
Summary: Manual review: Golgi membrane is consistent with known biology of VPS45.
Reason: Retained as supported or plausible for this gene and evidence context.
GO:0000149 SNARE binding
IPI
PMID:16769821
The Sec1p/Munc18 protein Vps45p binds its cognate SNARE prot...
ACCEPT
Summary: Manual review: SNARE binding is consistent with known biology of VPS45.
Reason: Retained as supported or plausible for this gene and evidence context.
GO:0005829 cytosol
IDA
PMID:9624182
The vesicle transport protein Vps33p is an ATP-binding prote...
ACCEPT
Summary: Manual review: cytosol is consistent with known biology of VPS45.
Reason: Retained as supported or plausible for this gene and evidence context.
GO:0006623 protein targeting to vacuole
IMP
PMID:7628704
STT10, a novel class-D VPS yeast gene required for osmotic i...
ACCEPT
Summary: Manual review: protein targeting to vacuole is consistent with known biology of VPS45.
Reason: Retained as supported or plausible for this gene and evidence context.
GO:0006895 Golgi to endosome transport
IGI
PMID:9335586
Genetic interactions between a pep7 mutation and the PEP12 a...
ACCEPT
Summary: Manual review: Golgi to endosome transport is consistent with known biology of VPS45.
Reason: Retained as supported or plausible for this gene and evidence context.
GO:0006896 Golgi to vacuole transport
IMP
PMID:7720726
Yeast Vps45p is a Sec1p-like protein required for the consum...
ACCEPT
Summary: Manual review: Golgi to vacuole transport is consistent with known biology of VPS45.
Reason: Retained as supported or plausible for this gene and evidence context.
GO:0007033 vacuole organization
IMP
PMID:9650782
Traffic into the prevacuolar/endosomal compartment of Saccha...
ACCEPT
Summary: Manual review: vacuole organization is consistent with known biology of VPS45.
Reason: Retained as supported or plausible for this gene and evidence context.
GO:0007035 vacuolar acidification
IMP
PMID:7628704
STT10, a novel class-D VPS yeast gene required for osmotic i...
ACCEPT
Summary: Manual review: vacuolar acidification is consistent with known biology of VPS45.
Reason: Retained as supported or plausible for this gene and evidence context.
GO:0031201 SNARE complex
IPI
PMID:10397773
A role for Tlg1p in the transport of proteins within the Gol...
ACCEPT
Summary: Manual review: SNARE complex is consistent with known biology of VPS45.
Reason: Retained as supported or plausible for this gene and evidence context.
GO:0032258 cytoplasm to vacuole targeting by the Cvt pathway
IMP
PMID:10545112
Cytoplasm to vacuole trafficking of aminopeptidase I require...
ACCEPT
Summary: Manual review: cytoplasm to vacuole targeting by the Cvt pathway is consistent with known biology of VPS45.
Reason: Retained as supported or plausible for this gene and evidence context.
GO:0035543 positive regulation of SNARE complex assembly
IMP
PMID:11432826
Vps45p stabilizes the syntaxin homologue Tlg2p and positivel...
ACCEPT
Summary: Manual review: positive regulation of SNARE complex assembly is consistent with known biology of VPS45.
Reason: Retained as supported or plausible for this gene and evidence context.
GO:0048210 Golgi vesicle fusion to target membrane
IMP
PMID:9650782
Traffic into the prevacuolar/endosomal compartment of Saccha...
ACCEPT
Summary: Manual review: Golgi vesicle fusion to target membrane is consistent with known biology of VPS45.
Reason: Retained as supported or plausible for this gene and evidence context.
GO:0051082 unfolded protein binding
IMP
PMID:11432826
Vps45p stabilizes the syntaxin homologue Tlg2p and positivel...
MARK AS OVER ANNOTATED
Summary: Manual review: unfolded protein binding is too generic or over-extended for VPS45.
Reason: Marked over-annotated because more specific terms capture the biology more accurately.
GO:0051082 unfolded protein binding
IPI
PMID:11432826
Vps45p stabilizes the syntaxin homologue Tlg2p and positivel...
MARK AS OVER ANNOTATED
Summary: Manual review: unfolded protein binding is too generic or over-extended for VPS45.
Reason: Marked over-annotated because more specific terms capture the biology more accurately.

References

Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot keyword mapping
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Electronic Gene Ontology annotations created by ARBA machine learning models
Combined Automated Annotation using Multiple IEA Methods
A role for Tlg1p in the transport of proteins within the Golgi apparatus of Saccharomyces cerevisiae.
Cytoplasm to vacuole trafficking of aminopeptidase I requires a t-SNARE-Sec1p complex composed of Tlg2p and Vps45p.
Pep3p/Pep5p complex: a putative docking factor at multiple steps of vesicular transport to the vacuole of Saccharomyces cerevisiae.
Vps45p stabilizes the syntaxin homologue Tlg2p and positively regulates SNARE complex formation.
A novel phospholipid-binding protein from the yeast Saccharomyces cerevisiae with dual binding specificities for the transport GTPase Ypt7p and the Sec1-related Vps33p.
Morphological classification of the yeast vacuolar protein sorting mutants: evidence for a prevacuolar compartment in class E vps mutants.
Proteome survey reveals modularity of the yeast cell machinery.
The Sec1p/Munc18 protein Vps45p binds its cognate SNARE proteins via two distinct modes.
High-quality binary protein interaction map of the yeast interactome network.
The N-terminal peptide of the syntaxin Tlg2p modulates binding of its closed conformation to Vps45p.
An inter-species protein-protein interaction network across vast evolutionary distance.
The social and structural architecture of the yeast protein interactome.
STT10, a novel class-D VPS yeast gene required for osmotic integrity related to the PKC1/STT1 protein kinase pathway.
Yeast Vps45p is a Sec1p-like protein required for the consumption of vacuole-targeted, post-Golgi transport vesicles.
Genetic interactions between a pep7 mutation and the PEP12 and VPS45 genes: evidence for a novel SNARE component in transport between the Saccharomyces cerevisiae Golgi complex and endosome.
The vesicle transport protein Vps33p is an ATP-binding protein that localizes to the cytosol in an energy-dependent manner.
Traffic into the prevacuolar/endosomal compartment of Saccharomyces cerevisiae: a VPS45-dependent intracellular route and a VPS45-independent, endocytic route.

📄 View Raw YAML

id: P38932
gene_symbol: VPS45
product_type: PROTEIN
status: DRAFT
taxon:
  id: NCBITaxon:559292
  label: Saccharomyces cerevisiae
description: >-
  VPS45 encodes a Sec1/Munc18 (SM) family protein essential for vacuolar
  protein sorting and membrane traffic between the late Golgi and the
  prevacuolar compartment. Vps45p binds and regulates the SNARE proteins
  Pep12p (a prevacuolar t-SNARE) and Tlg2p (a late Golgi/early endosome
  t-SNARE), promoting their proper function in SNARE complex assembly and
  membrane fusion. It is required for fusion of Golgi-derived transport
  vesicles with the prevacuolar compartment and also contributes to vacuole
  inheritance. Mutations in human VPS45 cause severe congenital neutropenia,
  underscoring the conserved importance of this trafficking regulator.
  Orthologous to human VPS45.
existing_annotations:
- term:
    id: GO:0016192
    label: vesicle-mediated transport
  evidence_type: IBA
  original_reference_id: GO_REF:0000033
  review:
    summary: 'Manual review: vesicle-mediated transport is consistent with known biology of VPS45.'
    action: ACCEPT
    reason: Retained as supported or plausible for this gene and evidence context.
- term:
    id: GO:0000139
    label: Golgi membrane
  evidence_type: IBA
  original_reference_id: GO_REF:0000033
  review:
    summary: 'Manual review: Golgi membrane is consistent with known biology of VPS45.'
    action: ACCEPT
    reason: Retained as supported or plausible for this gene and evidence context.
- term:
    id: GO:0006886
    label: intracellular protein transport
  evidence_type: IBA
  original_reference_id: GO_REF:0000033
  review:
    summary: 'Manual review: intracellular protein transport is consistent with known biology of VPS45.'
    action: ACCEPT
    reason: Retained as supported or plausible for this gene and evidence context.
- term:
    id: GO:0005737
    label: cytoplasm
  evidence_type: IEA
  original_reference_id: GO_REF:0000044
  review:
    summary: 'Manual review: cytoplasm is consistent with known biology of VPS45.'
    action: ACCEPT
    reason: Retained as supported or plausible for this gene and evidence context.
- term:
    id: GO:0005774
    label: vacuolar membrane
  evidence_type: IEA
  original_reference_id: GO_REF:0000044
  review:
    summary: 'Manual review: vacuolar membrane is consistent with known biology of VPS45.'
    action: ACCEPT
    reason: Retained as supported or plausible for this gene and evidence context.
- term:
    id: GO:0005794
    label: Golgi apparatus
  evidence_type: IEA
  original_reference_id: GO_REF:0000117
  review:
    summary: 'Manual review: Golgi apparatus is consistent with known biology of VPS45.'
    action: ACCEPT
    reason: Retained as supported or plausible for this gene and evidence context.
- term:
    id: GO:0006886
    label: intracellular protein transport
  evidence_type: IEA
  original_reference_id: GO_REF:0000117
  review:
    summary: 'Manual review: intracellular protein transport is consistent with known biology of VPS45.'
    action: ACCEPT
    reason: Retained as supported or plausible for this gene and evidence context.
- term:
    id: GO:0015031
    label: protein transport
  evidence_type: IEA
  original_reference_id: GO_REF:0000043
  review:
    summary: 'Manual review: protein transport is consistent with known biology of VPS45.'
    action: ACCEPT
    reason: Retained as supported or plausible for this gene and evidence context.
- term:
    id: GO:0016192
    label: vesicle-mediated transport
  evidence_type: IEA
  original_reference_id: GO_REF:0000120
  review:
    summary: 'Manual review: vesicle-mediated transport is consistent with known biology of VPS45.'
    action: ACCEPT
    reason: Retained as supported or plausible for this gene and evidence context.
- term:
    id: GO:0031201
    label: SNARE complex
  evidence_type: IEA
  original_reference_id: GO_REF:0000117
  review:
    summary: 'Manual review: SNARE complex is consistent with known biology of VPS45.'
    action: ACCEPT
    reason: Retained as supported or plausible for this gene and evidence context.
- term:
    id: GO:0031338
    label: regulation of vesicle fusion
  evidence_type: IEA
  original_reference_id: GO_REF:0000117
  review:
    summary: 'Manual review: regulation of vesicle fusion is consistent with known biology of VPS45.'
    action: ACCEPT
    reason: Retained as supported or plausible for this gene and evidence context.
- term:
    id: GO:0098588
    label: bounding membrane of organelle
  evidence_type: IEA
  original_reference_id: GO_REF:0000117
  review:
    summary: 'Manual review: bounding membrane of organelle is consistent with known biology of VPS45.'
    action: ACCEPT
    reason: Retained as supported or plausible for this gene and evidence context.
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:10397773
  review:
    summary: 'Manual review: protein binding is too generic or over-extended for VPS45.'
    action: MARK_AS_OVER_ANNOTATED
    reason: Marked over-annotated because more specific terms capture the biology more accurately.
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:10978279
  review:
    summary: 'Manual review: protein binding is too generic or over-extended for VPS45.'
    action: MARK_AS_OVER_ANNOTATED
    reason: Marked over-annotated because more specific terms capture the biology more accurately.
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:12553664
  review:
    summary: 'Manual review: protein binding is too generic or over-extended for VPS45.'
    action: MARK_AS_OVER_ANNOTATED
    reason: Marked over-annotated because more specific terms capture the biology more accurately.
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:16429126
  review:
    summary: 'Manual review: protein binding is too generic or over-extended for VPS45.'
    action: MARK_AS_OVER_ANNOTATED
    reason: Marked over-annotated because more specific terms capture the biology more accurately.
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:18719252
  review:
    summary: 'Manual review: protein binding is too generic or over-extended for VPS45.'
    action: MARK_AS_OVER_ANNOTATED
    reason: Marked over-annotated because more specific terms capture the biology more accurately.
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:19667197
  review:
    summary: 'Manual review: protein binding is too generic or over-extended for VPS45.'
    action: MARK_AS_OVER_ANNOTATED
    reason: Marked over-annotated because more specific terms capture the biology more accurately.
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:27107014
  review:
    summary: 'Manual review: protein binding is too generic or over-extended for VPS45.'
    action: MARK_AS_OVER_ANNOTATED
    reason: Marked over-annotated because more specific terms capture the biology more accurately.
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:37968396
  review:
    summary: 'Manual review: protein binding is too generic or over-extended for VPS45.'
    action: MARK_AS_OVER_ANNOTATED
    reason: Marked over-annotated because more specific terms capture the biology more accurately.
- term:
    id: GO:0000011
    label: vacuole inheritance
  evidence_type: IMP
  original_reference_id: PMID:1493335
  review:
    summary: 'Manual review: vacuole inheritance is consistent with known biology of VPS45.'
    action: ACCEPT
    reason: Retained as supported or plausible for this gene and evidence context.
- term:
    id: GO:0000139
    label: Golgi membrane
  evidence_type: IDA
  original_reference_id: PMID:7720726
  review:
    summary: 'Manual review: Golgi membrane is consistent with known biology of VPS45.'
    action: ACCEPT
    reason: Retained as supported or plausible for this gene and evidence context.
- term:
    id: GO:0000149
    label: SNARE binding
  evidence_type: IPI
  original_reference_id: PMID:16769821
  review:
    summary: 'Manual review: SNARE binding is consistent with known biology of VPS45.'
    action: ACCEPT
    reason: Retained as supported or plausible for this gene and evidence context.
- term:
    id: GO:0005829
    label: cytosol
  evidence_type: IDA
  original_reference_id: PMID:9624182
  review:
    summary: 'Manual review: cytosol is consistent with known biology of VPS45.'
    action: ACCEPT
    reason: Retained as supported or plausible for this gene and evidence context.
- term:
    id: GO:0006623
    label: protein targeting to vacuole
  evidence_type: IMP
  original_reference_id: PMID:7628704
  review:
    summary: 'Manual review: protein targeting to vacuole is consistent with known biology of VPS45.'
    action: ACCEPT
    reason: Retained as supported or plausible for this gene and evidence context.
- term:
    id: GO:0006895
    label: Golgi to endosome transport
  evidence_type: IGI
  original_reference_id: PMID:9335586
  review:
    summary: 'Manual review: Golgi to endosome transport is consistent with known biology of VPS45.'
    action: ACCEPT
    reason: Retained as supported or plausible for this gene and evidence context.
- term:
    id: GO:0006896
    label: Golgi to vacuole transport
  evidence_type: IMP
  original_reference_id: PMID:7720726
  review:
    summary: 'Manual review: Golgi to vacuole transport is consistent with known biology of VPS45.'
    action: ACCEPT
    reason: Retained as supported or plausible for this gene and evidence context.
- term:
    id: GO:0007033
    label: vacuole organization
  evidence_type: IMP
  original_reference_id: PMID:9650782
  review:
    summary: 'Manual review: vacuole organization is consistent with known biology of VPS45.'
    action: ACCEPT
    reason: Retained as supported or plausible for this gene and evidence context.
- term:
    id: GO:0007035
    label: vacuolar acidification
  evidence_type: IMP
  original_reference_id: PMID:7628704
  review:
    summary: 'Manual review: vacuolar acidification is consistent with known biology of VPS45.'
    action: ACCEPT
    reason: Retained as supported or plausible for this gene and evidence context.
- term:
    id: GO:0031201
    label: SNARE complex
  evidence_type: IPI
  original_reference_id: PMID:10397773
  review:
    summary: 'Manual review: SNARE complex is consistent with known biology of VPS45.'
    action: ACCEPT
    reason: Retained as supported or plausible for this gene and evidence context.
- term:
    id: GO:0032258
    label: cytoplasm to vacuole targeting by the Cvt pathway
  evidence_type: IMP
  original_reference_id: PMID:10545112
  review:
    summary: 'Manual review: cytoplasm to vacuole targeting by the Cvt pathway is consistent with known biology of VPS45.'
    action: ACCEPT
    reason: Retained as supported or plausible for this gene and evidence context.
- term:
    id: GO:0035543
    label: positive regulation of SNARE complex assembly
  evidence_type: IMP
  original_reference_id: PMID:11432826
  review:
    summary: 'Manual review: positive regulation of SNARE complex assembly is consistent with known biology of VPS45.'
    action: ACCEPT
    reason: Retained as supported or plausible for this gene and evidence context.
- term:
    id: GO:0048210
    label: Golgi vesicle fusion to target membrane
  evidence_type: IMP
  original_reference_id: PMID:9650782
  review:
    summary: 'Manual review: Golgi vesicle fusion to target membrane is consistent with known biology of VPS45.'
    action: ACCEPT
    reason: Retained as supported or plausible for this gene and evidence context.
- term:
    id: GO:0051082
    label: unfolded protein binding
  evidence_type: IMP
  original_reference_id: PMID:11432826
  review:
    summary: 'Manual review: unfolded protein binding is too generic or over-extended for VPS45.'
    action: MARK_AS_OVER_ANNOTATED
    reason: Marked over-annotated because more specific terms capture the biology more accurately.
- term:
    id: GO:0051082
    label: unfolded protein binding
  evidence_type: IPI
  original_reference_id: PMID:11432826
  review:
    summary: 'Manual review: unfolded protein binding is too generic or over-extended for VPS45.'
    action: MARK_AS_OVER_ANNOTATED
    reason: Marked over-annotated because more specific terms capture the biology more accurately.
references:
- id: GO_REF:0000033
  title: Annotation inferences using phylogenetic trees
  findings: []
- id: GO_REF:0000043
  title: Gene Ontology annotation based on UniProtKB/Swiss-Prot keyword mapping
  findings: []
- id: GO_REF:0000044
  title: Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
  findings: []
- id: GO_REF:0000117
  title: Electronic Gene Ontology annotations created by ARBA machine learning models
  findings: []
- id: GO_REF:0000120
  title: Combined Automated Annotation using Multiple IEA Methods
  findings: []
- id: PMID:10397773
  title: A role for Tlg1p in the transport of proteins within the Golgi apparatus of Saccharomyces cerevisiae.
  findings: []
- id: PMID:10545112
  title: Cytoplasm to vacuole trafficking of aminopeptidase I requires a t-SNARE-Sec1p complex composed of Tlg2p and Vps45p.
  findings: []
- id: PMID:10978279
  title: 'Pep3p/Pep5p complex: a putative docking factor at multiple steps of vesicular transport to the vacuole of Saccharomyces cerevisiae.'
  findings: []
- id: PMID:11432826
  title: Vps45p stabilizes the syntaxin homologue Tlg2p and positively regulates SNARE complex formation.
  findings: []
- id: PMID:12553664
  title: A novel phospholipid-binding protein from the yeast Saccharomyces cerevisiae with dual binding specificities for the transport GTPase Ypt7p and the Sec1-related Vps33p.
  findings: []
- id: PMID:1493335
  title: 'Morphological classification of the yeast vacuolar protein sorting mutants: evidence for a prevacuolar compartment in class E vps mutants.'
  findings: []
- id: PMID:16429126
  title: Proteome survey reveals modularity of the yeast cell machinery.
  findings: []
- id: PMID:16769821
  title: The Sec1p/Munc18 protein Vps45p binds its cognate SNARE proteins via two distinct modes.
  findings: []
- id: PMID:18719252
  title: High-quality binary protein interaction map of the yeast interactome network.
  findings: []
- id: PMID:19667197
  title: The N-terminal peptide of the syntaxin Tlg2p modulates binding of its closed conformation to Vps45p.
  findings: []
- id: PMID:27107014
  title: An inter-species protein-protein interaction network across vast evolutionary distance.
  findings: []
- id: PMID:37968396
  title: The social and structural architecture of the yeast protein interactome.
  findings: []
- id: PMID:7628704
  title: STT10, a novel class-D VPS yeast gene required for osmotic integrity related to the PKC1/STT1 protein kinase pathway.
  findings: []
- id: PMID:7720726
  title: Yeast Vps45p is a Sec1p-like protein required for the consumption of vacuole-targeted, post-Golgi transport vesicles.
  findings: []
- id: PMID:9335586
  title: 'Genetic interactions between a pep7 mutation and the PEP12 and VPS45 genes: evidence for a novel SNARE component in transport between the Saccharomyces cerevisiae Golgi complex and endosome.'
  findings: []
- id: PMID:9624182
  title: The vesicle transport protein Vps33p is an ATP-binding protein that localizes to the cytosol in an energy-dependent manner.
  findings: []
- id: PMID:9650782
  title: 'Traffic into the prevacuolar/endosomal compartment of Saccharomyces cerevisiae: a VPS45-dependent intracellular route and a VPS45-independent, endocytic route.'
  findings: []