family_id: PANTHER:PTHR12632
family_name: TRANSCRIPTION FACTOR NF-Y ALPHA-RELATED
preferred_name: NF-YA/Hap2 CCAAT-binding complex subunits
summary: NF-YA/Hap2 proteins form the sequence-recognition component of the heteromeric CCAAT-binding
  transcription factor. DNA binding and transcriptional regulation depend on assembly with the other NF-Y
  subunits. Fungal iron and oxidative-stress responses and plant developmental functions represent deployment
  of this conserved transcriptional machinery in different regulatory networks.
functional_coherence: COHERENT
coherence_reason: The inventory consistently identifies NF-YA/Hap2, the CCAAT-recognition subunit of a
  heteromeric transcription factor. Curated IPR001289 separates its subunit-association and DNA-recognition
  regions. DNA recognition and transcriptional regulation require the assembled NF-Y/Hap complex; mitochondrial
  respiratory targets in yeast or iron-responsive circuits in particular fungi are not universal target
  regulons.
subfamilies:
- label: NF-YA/Hap2 CCAAT-binding complex subunits
  description: The inventory consistently contains NF-YA or Hap2 proteins. The relevant conserved property
    is CCAAT-binding complex participation, not a particular iron-responsive circuit or an ability to
    bind promoter DNA as an isolated polypeptide.
  representative_members:
  - id: UniProtKB:Q7SGY4
    gene_symbol: NCU03033
    organism: Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
    label: Transcriptional activator HAP2
    gene_review: genes/NEUCR/NCU03033/NCU03033-ai-review.yaml
term_assessments:
- assessed_term:
    id: GO:0006355
    label: regulation of DNA-templated transcription
  scope: FAMILY_WIDE
  scope_reason: The inventory consistently contains NF-YA or Hap2 proteins. The relevant conserved property
    is CCAAT-binding complex participation, not a particular iron-responsive circuit or an ability to
    bind promoter DNA as an isolated polypeptide.
  supported_by:
  - reference_id: file:projects/PROTNLM_EVALUATION/family-curation/evidence-c/PTHR12632.md
    supporting_text: 'Diverse DNA binding proteins are known to bind the CCAAT box, a common cis- acting
      element found in the promoter and enhancer regions of a large number of genes in eukaryotes. Amongst
      these proteins is one known as the CCAAT-binding factor (CBF) or nuclear transcription factor Y
      (NF-Y) [[cite:PUB00004401]]. CBF is a heteromeric transcription factor that consists of two different
      components both needed for DNA-binding.


      The HAP protein complex of yeast binds to the upstream activation site of cytochrome C iso-1 gene
      (CYC1) as well as other genes involved in mitochondrial electron transport and activates their expression.
      It also recognises the sequence CCAAT and is structurally and evolutionary related to CBF.


      The first subunit of CBF is known as CBF-A or NF-YB in vertebrates, and HAP3 in budding yeast. The
      second subunit is known as CBF-B or NF-YA in vertebrates and HAP2 in budding yeast. It is a protein
      of 265 to 350 amino-acid residues which contains a highly conserved region of about 60 residues.
      This region, called the ''essential core'' [[cite:PUB00003675]], seems to consist of two subdomains:
      an N-terminal subunit-association domain and a C-terminal DNA recognition domain.


      This entry represents the NF-YA subunit.'
references:
- id: file:projects/PROTNLM_EVALUATION/family-curation/evidence-c/PTHR12632.md
  title: PANTHER/InterPro and UniProt source extracts for PTHR12632
- id: file:interpro/panther/PTHR12632/PTHR12632-entries.csv
  title: PTHR12632 cached PANTHER member inventory
review_status: COMPLETE
reviewed_by: codex
review_date: '2026-09-10'
notes: Family and subfamily names are read from panther.obo; representative assignments are checked against
  the frozen UniProt PANTHER cross-references and available member inventory. Source provenance and sequence
  lengths are recorded in projects/PROTNLM_EVALUATION/family-curation/evidence-c/PTHR12632.md. Functional
  statements derived from ARBA and LLM-generated descriptions are not independent validation. No PAINT
  ancestral-node placement or residue-loss assertion is made without the corresponding phylogenetic or
  sequence analysis.