Bacterial ethanolamine uptake and cobalamin-dependent catabolism

A reusable bacterial pathway in which ethanolamine uptake supplies the adenosylcobalamin-dependent EutB/EutC ammonia-lyase, producing acetaldehyde and ammonium. Acetaldehyde is then oxidized either to acetyl-CoA by an acetylating EutE-like enzyme or to acetate by a non-acylating NAD-dependent aldehyde dehydrogenase. Transporter family and microcompartment encapsulation vary between organisms; shell assembly, cobalamin supply, regulation, and downstream assimilation are outside the core boundary.

MODULE:bacterial_ethanolamine_uptake_catabolismDRAFTCONCRETEMetabolic Pathwaymodules/bacterial_ethanolamine_uptake_catabolism.yaml
ethanolamine catabolic processGO:0046336
PMID:19783625
Comparative genomics of ethanolamine utilization.
Establishes EutBC as the conserved ethanolamine-to-acetaldehyde-and-ammonia step and documents evolutionary variation in auxiliary eut genes and metabolosomes.
PMID:19762342
Purification and some properties of wild-type and N-terminal-truncated ethanolamine ammonia-lyase of Escherichia coli.
Directly establishes EutB/EutC large-small subunit composition and alpha6-beta6 assembly.
PMID:20519496
Crystal structures of ethanolamine ammonia-lyase complexed with coenzyme B12 analogs and substrates.
Resolves the active-site barrel in EutB and the interfacial cobalamin-capping role of EutC.
PMID:7868611
Ethanolamine utilization in Salmonella typhimurium: nucleotide sequence, protein expression, and mutational analysis of the cchA cchB eutE eutJ eutG eutH gene cluster.
Supports transport and acetaldehyde-disposition roles in a characterized shell-encoding eut operon.
file:PSEPK/eutC/eutC-deep-research-openscientist.md
OpenScientist gene research for PSEPK EutC
Corroborates the EutBC chemistry and subunit structure. Its unaccompanied proteome-wide Pfam absence claim is not used as curation evidence.
file:projects/P_PUTIDA/data/psepk_pathway_partition.tsv
PSEPK pathway partition
Provides exact KT2440 accessions and exposes the misleading KEGG ppu00564 placement of eutBC, which is treated as a database bucket rather than the pathway boundary.

Molecular functions occur only on leaf annotons. EutB and EutC are active units of one enzyme-complex annoton; individual gene reviews use contributes_to qualifiers. KT2440 exemplifies an APC-family transporter and has an adjacent non-acylating AldB/AcoD-like candidate, not classical EutH or acetylating EutE; its acetaldehyde specificity remains unverified. Microcompartment encapsulation is an optional implementation context and is not required by the pathway definition. RHEA:23288 and the RHEA:25294 master reaction were verified against Rhea. The GOA source PTN PTN002446609 was verified exactly from Q88QF2 GOA. Canonical local PAINT data instead place GO:0009350 at PTN002217404 in PTHR39330, seeded by P19636 and P19265; only that canonical node is used as an ancestral-node assertion.

8Nodes
3Parts
2Variant Sets
4Variants
5Annotons
2Connections

Derived QC

Recommended-field compliance

30.8% recommended fields populated
  • module.knowledge_gaps[0] · boundary (0/1)
  • module.knowledge_gaps[0] · status (0/1)
  • module.knowledge_gaps[0] · provenance (0/1)
  • module.knowledge_gaps[1] · boundary (0/1)
  • module.knowledge_gaps[1] · status (0/1)
  • module.knowledge_gaps[1] · provenance (0/1)
  • module.knowledge_gaps[2] · boundary (0/1)
  • module.knowledge_gaps[2] · status (0/1)
  • module.knowledge_gaps[2] · provenance (0/1)

Module deep research

✗ none found

No MODULE:bacterial_ethanolamine_uptake_catabolism deep-research report alongside the module YAML.

Leaf nodes lacking representative members

✓ every leaf node grounds to a representative protein.

Template conformance

✓ every declared conforms_to bundle matches its template motif.

Gene-review completeness (3/8 grounded genes reviewed)

1 complete review(s) · 1 with deep research · 5 missing review · 2 reviewed but lacking deep research

Gene Review Complete Deep research
eutB Q88QF1 ✓ 8/10 ✗
eutC Q88QF2 ✓ 7/9 ✓
EutB (Escherichia coli K-12) P0AEJ6 ✗ — —
EutC (Escherichia coli K-12) P19636 ✗ — —
EutE (Salmonella Typhimurium LT2) P41793 ✗ — —
EutH (Salmonella Typhimurium LT2) P41796 ✗ — —
PP_0544 Q88QF0 ✓ ✓ ✗
ExaC (Pseudomonas aeruginosa) Q9ZAA1 ✗ — —

Details

Context
bacteriaNCBITaxon:2
Bacterial ethanolamine uptake and cobalamin-dependent catabolismMetabolic Pathwaybacterial_ethanolamine_uptake_catabolism
ethanolamine catabolic processGO:0046336
Context
bacteriaNCBITaxon:2

Connections

Uptake supplies intracellular ethanolamine to EutB/EutC.
EutB/EutC supplies acetaldehyde to either downstream oxidation route.
Part 1: ethanolamine uptake
Ethanolamine transmembrane uptakeTransport Stepethanolamine_uptake
Variant set: Ethanolamine transporter implementations by transporter family (One Or More)
Classical EutH-family uptakeTransport Stepclassical_euth_transport

Annotons

EutH ethanolamine permease
euth_transport_activity
Participant: Family: EutH ethanolamine permease family
Family:
EutH ethanolamine permease familyPANTHER:PTHR40089:SF1
Representative Members: EutH (Salmonella Typhimurium LT2)UniProtKB:P41796

Function

ethanolamine transmembrane transporter activityGO:0034228
Substrates: extracellular ethanolamine
Products: intracellular ethanolamine

Locations

plasma membraneGO:0005886

Classical EutH implementation found in enterobacterial eut loci.

APC-family ethanolamine-permease uptakeTransport Stepapc_ethanolamine_permease_transport

Annotons

APC-family ethanolamine permease
apc_ethanolamine_transport_activity
Participant: Family: APC-superfamily ethanolamine permeases
Family:
APC-superfamily ethanolamine permeasesInterPro:IPR004757
Representative Members: PP_0544 (Pseudomonas putida KT2440)UniProtKB:Q88QF0

Function

ethanolamine transmembrane transporter activityGO:0034228
Substrates: extracellular ethanolamine
Products: intracellular ethanolamine

Locations

plasma membraneGO:0005886

Alternative 12-pass APC-family implementation represented by the IPR004757/TIGR00908 KT2440 candidate; direct transport kinetics remain needed.

Part 2: cobalamin-dependent ethanolamine deamination
EutB/EutC ethanolamine ammonia-lyase reactionReactioneutbc_ethanolamine_deamination

Annotons

Cobalamin-dependent EutB/EutC ammonia-lyase
eutbc_complex_activity
Participant: Protein Complex: bacterial ethanolamine ammonia-lyase complex
Protein Complex:
bacterial ethanolamine ammonia-lyase complexGO:0009350
Active units:
EutB large subunit
Participant: Family: EutB ethanolamine ammonia-lyase large-subunit family
Family:
EutB ethanolamine ammonia-lyase large-subunit familyPANTHER:PTHR39329:SF1
Representative Members: EutB (Pseudomonas putida KT2440)UniProtKB:Q88QF1 EutB (Escherichia coli K-12)UniProtKB:P0AEJ6
Role: Catalytic large subunit containing the active-site TIM barrel.
EutC small subunit
Participant: Family: EutC ethanolamine ammonia-lyase small-subunit family
Family:
EutC ethanolamine ammonia-lyase small-subunit familyPANTHER:PTHR39330
Representative Members: EutC (Pseudomonas putida KT2440)UniProtKB:Q88QF2 EutC (Escherichia coli K-12)UniProtKB:P19636
Role: Small subunit completing the interfacial cobalamin environment.

Function

ethanolamine ammonia-lyase activityGO:0008851
Substrates: ethanolamine
Products: acetaldehyde ammonium

The assembled EutB/EutC complex performs the adenosylcobalamin-dependent pathway-defining cleavage; neither subunit independently enables the activity.

Part 3: acetaldehyde oxidation
Oxidation of EutBC-derived acetaldehydeMetabolic Pathwayacetaldehyde_oxidation
Variant set: Acetaldehyde oxidation routes by carbon product and CoA dependence (One Or More)
Acetylating EutE route to acetyl-CoAReactionacetylating_eute_route

Annotons

EutE acetaldehyde dehydrogenase (acetylating)
eute_acetylating_activity
Participant: Family: acetylating EutE family
Family:
acetylating EutE familyPANTHER:PTHR11699:SF68 P41793 is indexed in the EutE-specific PTHR11699:SF68 subfamily. This selector applies only to the acetylating acetaldehyde-to-acetyl-CoA variant, not to non-acylating aldehyde dehydrogenases.
Representative Members: EutE (Salmonella Typhimurium LT2)UniProtKB:P41793

Function

acetaldehyde dehydrogenase (acetylating) activityGO:0008774
Substrates: acetaldehyde coenzyme A NAD+
Products: acetyl-CoA NADH proton

Classical EutE route that conserves the acetaldehyde carbon as acetyl-CoA.

Non-acylating ALDH route to acetateReactionnon_acylating_aldh_route

Annotons

Non-acylating NAD-dependent acetaldehyde dehydrogenase
aldb_acetaldehyde_dehydrogenase_activity
Participant: Family: AldB/AcoD-like non-acylating aldehyde dehydrogenases
Family:
AldB/AcoD-like non-acylating aldehyde dehydrogenasesPANTHER:PTHR43111:SF1
Representative Members: ExaC (Pseudomonas aeruginosa)UniProtKB:Q9ZAA1

Function

acetaldehyde dehydrogenase (NAD+) activityGO:0140087
Substrates: acetaldehyde NAD+ water
Products: acetate NADH protons

Alternative non-acylating route represented by biochemically characterized ExaC. Whether KT2440 AldB-I implements this route is unresolved.