Bacterial flagellar assembly and type III export

Reusable gammaproteobacterial module spanning basal-body and switch construction, flagellar type III export, rod-hook assembly and length control, late transcriptional staging, filament completion, and proton- coupled stator installation. Exact Pseudomonas putida representatives ground the architectural parts without making the module specific to its locus.

MODULE:bacterial_flagellar_assembly_exportDRAFTCONCRETEBiological Processmodules/bacterial_flagellar_assembly_export.yaml
bacterial-type flagellum assemblyGO:0044780
GO:0044780
bacterial-type flagellum assembly
Grounds ordered construction of the bacterial flagellum.
KEGG:ppu02040
Pseudomonas putida KT2440 flagellar assembly
Identifies the PSEPK regulatory, export, structural, and motor realization.
file:projects/P_PUTIDA/deep-research/PSEPK__bacterial_flagellar_assembly_export__ppu02040-deep-research-openscientist.md
OpenScientist PSEPK flagellar assembly pathway review
The species-aware report finds all six architectural parts represented, identifies omitted export, switch, rod, and junction subunits, and flags KEGG name-collision and regulatory-boundary issues.

The module captures a representative architectural panel, not a claim that every protein required to construct a flagellum has been curated. Additional rod, ring, junction, chaperone, and export-gate proteins in ppu02040 remain outside this focused review boundary. Chemotactic control of rotation is modeled in the bacterial chemotaxis signal-transduction module. Molecular functions are not assigned to structural subunits merely from complex membership. FliA/FlgM staging and proton-coupled MotAB are scoped to the gammaproteobacterial realization rather than asserted as universal bacterial requirements.

7Nodes
6Parts
0Variant Sets
0Variants
27Annotons
6Connections

Derived QC

Recommended-field compliance

100.0% recommended fields populated

All recommended fields populated.

Module deep research

✗ none found

No MODULE:bacterial_flagellar_assembly_export deep-research report alongside the module YAML.

Leaf nodes lacking representative members

✓ every leaf node grounds to a representative protein.

Template conformance

✓ every declared conforms_to bundle matches its template motif.

Reaction chaining (advisory)

✓ every PRECEDES step chains, or its break is acknowledged via chaining_status.

  • ms_ring_switch_assembly → flagellar_type_iii_export [NOT_CHECKED]
  • rod_hook_assembly → filament_completion [NOT_CHECKED]
  • ms_ring_switch_assembly → motab_stator_installation [NOT_CHECKED]

Gene-review completeness (16/27 grounded genes reviewed)

16 complete review(s) · 16 with deep research · 11 missing review · 0 reviewed but lacking deep research

Gene Review Complete Deep research
flgE Q88ER5 ✓ ✓ ✓
flgG Q88ER8 ✓ ✓ ✓
flgK Q88ES2 ✓ ✓ ✓
flgL Q88ES3 ✓ ✓ ✓
flgM Q88EQ8 ✓ ✓ ✓
flhA Q88EV8 ✓ ✓ ✓
fliA Q88EW1 ✓ ✓ ✓
fliC Q88ES5 ✓ ✓ ✓
fliD Q88ES7 ✓ ✓ ✓
fliF Q88ET4 ✓ ✓ ✓
fliG Q88ET5 ✓ ✓ ✓
fliI Q88ET7 ✓ ✓ ✓
fliK Q88EU2 ✓ ✓ ✓
fliP Q88EU8 ✓ ✓ ✓
motA Q88DC2 ✓ ✓ ✓
motB Q88DC3 ✓ ✓ ✓
PSEPK FlgB exemplar Q88ER2 ✗ — —
PSEPK FlgC exemplar Q88ER3 ✗ — —
PSEPK FlgD exemplar Q88ER4 ✗ — —
PSEPK FlgF exemplar Q88ER7 ✗ — —
PSEPK FliH exemplar Q88ET6 ✗ — —
PSEPK FliJ exemplar Q88ET8 ✗ — —
PSEPK FliM exemplar Q88EU5 ✗ — —
PSEPK FliN exemplar Q88EU6 ✗ — —
PSEPK FliQ exemplar Q88EU9 ✗ — —
PSEPK FliR exemplar Q88EV0 ✗ — —
PSEPK FlhB exemplar Q88EV1 ✗ — —

Details

Context
GammaproteobacteriaNCBITaxon:1236
Bacterial flagellar assembly and exportBiological Processbacterial_flagellar_assembly_export
bacterial-type flagellum assemblyGO:0044780
Context
GammaproteobacteriaNCBITaxon:1236

Connections

Part 4: late flagellar transcription control
FliA and FlgM transcriptional checkpointRegulatory Stepflagellar_late_transcription

Annotons

FliA sigma factor
flia_sigma_factor
Participant: Family: flagellar sigma factor FliA family
Family:
flagellar sigma factor FliA family
Representative Members: PSEPK FliA exemplarUniProtKB:Q88EW1

Directs late flagellar gene transcription after assembly reaches the secretion checkpoint.

FlgM anti-sigma factor
flgm_antisigma_factor
Participant: Family: flagellar anti-sigma factor FlgM family
Family:
flagellar anti-sigma factor FlgM family
Representative Members: PSEPK FlgM exemplarUniProtKB:Q88EQ8

Restrains FliA until the hook-basal-body export checkpoint is satisfied.

Part 2: flagellar type III export
Membrane export gate and cytosolic ATPaseProtein Complexflagellar_type_iii_export

Annotons

FlhA export-gate component
flha_export_gate
Participant: Family: FlhA family
Family:
FlhA familyPANTHER:PTHR30161:SF1
Representative Members: PSEPK FlhA exemplarUniProtKB:Q88EV8

Major membrane component coordinating substrate export and assembly state.

FliP export-gate component
flip_export_gate
Participant: Family: FliP family
Family:
FliP familyPANTHER:PTHR30587:SF0
Representative Members: PSEPK FliP exemplarUniProtKB:Q88EU8

Conserved membrane export-gate component.

FlhB export-switch component
flhb_export_switch
Participant: Family: FlhB family
Family:
FlhB familyPANTHER:PTHR30531:SF12
Representative Members: PSEPK FlhB exemplarUniProtKB:Q88EV1

Participates in export-substrate specificity switching.

FliQ export-gate component
fliq_export_gate
Participant: Family: FliQ family
Family:
FliQ familyPANTHER:PTHR34040:SF8
Representative Members: PSEPK FliQ exemplarUniProtKB:Q88EU9

Forms part of the membrane export-gate core.

FliR export-gate component
flir_export_gate
Participant: Family: FliR family
Family:
FliR familyPANTHER:PTHR30065:SF8
Representative Members: PSEPK FliR exemplarUniProtKB:Q88EV0

Forms part of the membrane export-gate core.

FliI export ATPase
flii_export_atpase
Participant: Family: FliI flagellar export ATPase family
Family:
FliI flagellar export ATPase family
Representative Members: PSEPK FliI exemplarUniProtKB:Q88ET7

Supplies ATP-dependent substrate handling at the export apparatus.

FliH export ATPase regulator
flih_atpase_regulator
Participant: Family: FliH family
Family:
FliH familyPANTHER:PTHR34982:SF1
Representative Members: PSEPK FliH exemplarUniProtKB:Q88ET6

Regulates and positions the FliI ATPase at the export apparatus.

FliJ export apparatus component
flij_export_chaperone
Participant: Family: FliJ family
Family:
FliJ familyPANTHER:PTHR38786:SF1
Representative Members: PSEPK FliJ exemplarUniProtKB:Q88ET8

Couples export substrates and chaperones to the export ATPase apparatus.

Part 1: basal-body MS ring and switch assembly
FliF MS ring and FliG switchProtein Complexms_ring_switch_assembly

Annotons

FliF MS-ring component
flif_ms_ring
Participant: Family: FliF family
Family:
FliF familyPANTHER:PTHR30046:SF0
Representative Members: PSEPK FliF exemplarUniProtKB:Q88ET4

Forms the membrane-embedded MS ring that anchors export and switch components.

FliG switch component
flig_switch
Participant: Family: FliG family
Family:
FliG familyPANTHER:PTHR30534
Representative Members: PSEPK FliG exemplarUniProtKB:Q88ET5

Couples the rotor to stator torque and chemotactic switching.

FliM switch component
flim_switch
Participant: Family: FliM family
Family:
FliM familyPANTHER:PTHR30034:SF3
Representative Members: PSEPK FliM exemplarUniProtKB:Q88EU5

Forms the C-ring switch and receives chemotactic switching inputs.

FliN switch component
flin_switch
Participant: Family: FliN family
Family:
FliN familyPANTHER:PTHR43484:SF1
Representative Members: PSEPK FliN exemplarUniProtKB:Q88EU6

Forms the C-ring switch and supports rotor assembly.

Part 3: rod-hook construction and length control
Distal rod, hook, and hook-length checkpointBiological Processrod_hook_assembly

Annotons

FlgG distal rod
flgg_distal_rod
Participant: Family: FlgG family
Family:
FlgG familyPANTHER:PTHR30435:SF19
Representative Members: PSEPK FlgG exemplarUniProtKB:Q88ER8

Builds the distal rod through the periplasm.

FlgB proximal rod
flgb_proximal_rod
Participant: Family: FlgB family
Family:
FlgB familyPANTHER:PTHR30435:SF12
Representative Members: PSEPK FlgB exemplarUniProtKB:Q88ER2

Contributes to the proximal rod linking the MS ring to distal structures.

FlgC proximal rod
flgc_proximal_rod
Participant: Family: FlgC family
Family:
FlgC familyPANTHER:PTHR30435:SF29
Representative Members: PSEPK FlgC exemplarUniProtKB:Q88ER3

Contributes to the proximal rod linking the MS ring to distal structures.

FlgF rod component
flgf_rod
Participant: Family: FlgF family
Family:
FlgF familyPANTHER:PTHR30435:SF18
Representative Members: PSEPK FlgF exemplarUniProtKB:Q88ER7

Connects proximal and distal rod structures.

FlgD hook assembly cap
flgd_hook_cap
Participant: Family: FlgD hook assembly family
Family:
FlgD hook assembly family
Representative Members: PSEPK FlgD exemplarUniProtKB:Q88ER4

Acts as the hook assembly cap before filament-junction completion.

FlgE hook
flge_hook
Participant: Family: FlgE hook family
Family:
FlgE hook family
Representative Members: PSEPK FlgE exemplarUniProtKB:Q88ER5

Polymerizes the flexible hook joining rod and filament.

FliK hook-length control
flik_length_control
Participant: Family: FliK family
Family:
FliK familyPANTHER:PTHR37533:SF2
Representative Members: PSEPK FliK exemplarUniProtKB:Q88EU2

Triggers export-substrate switching when the hook reaches its target length.

Part 5: filament junction, polymerization, and capping
Hook-filament junction, flagellin filament, and capBiological Processfilament_completion

Annotons

FlgK hook-filament junction
flgk_junction
Participant: Family: FlgK family
Family:
FlgK family
Representative Members: PSEPK FlgK exemplarUniProtKB:Q88ES2

Forms the proximal hook-filament junction.

FliC flagellin
flic_flagellin
Participant: Family: bacterial flagellin family
Family:
bacterial flagellin family
Representative Members: PSEPK FliC exemplarUniProtKB:Q88ES5

Polymerizes into the extracellular flagellar filament.

FlgL hook-filament junction
flgl_junction
Participant: Family: FlgL family
Family:
FlgL familyPANTHER:PTHR42792:SF1
Representative Members: PSEPK FlgL exemplarUniProtKB:Q88ES3

Forms the distal hook-filament junction with FlgK.

FliD filament cap
flid_cap
Participant: Family: FliD cap family
Family:
FliD cap familyPANTHER:PTHR30288:SF0
Representative Members: PSEPK FliD exemplarUniProtKB:Q88ES7

Caps the distal filament and promotes flagellin incorporation.

Part 6: stator installation and torque generation
MotAB stator complexProtein Complexmotab_stator_installation

Annotons

MotA stator subunit
mota_stator
Participant: Family: MotA family
Family:
MotA familyPANTHER:PTHR30433
Representative Members: PSEPK MotA exemplarUniProtKB:Q88DC2

Membrane stator subunit contributing to proton-driven torque.

MotB stator subunit
motb_stator
Participant: Family: MotB family
Family:
MotB familyPANTHER:PTHR30329
Representative Members: PSEPK MotB exemplarUniProtKB:Q88DC3

Anchors the stator to peptidoglycan and contributes to proton-driven torque.