Bacterial glutamate-to-P5C branch of proline biosynthesis

Species-neutral bacterial module for the two-enzyme conversion of L-glutamate to L-glutamate 5-semialdehyde, which cyclizes to 1-pyrroline-5-carboxylate (P5C), during proline biosynthesis. ProB phosphorylates L-glutamate to L-glutamyl 5-phosphate, and ProA reduces that acyl phosphate intermediate to L-glutamate 5-semialdehyde using NADPH. This module deliberately stops before the terminal P5C reductase step that makes L-proline.

MODULE:bacterial_glutamate_to_p5c_biosynthesisDRAFTCONCRETEMetabolic Pathwaymodules/bacterial_glutamate_to_p5c_biosynthesis.yaml
L-proline biosynthetic processGO:0055129
KEGG:ppu00332
Pseudomonas putida KT2440 ProB/ProA bucket
The local PSEPK pathway partition assigns proB/PP_0691 and proA/PP_4811 as the two primary genes in this compact bucket. Although the KEGG label is carbapenem biosynthesis, the PSEPK UniProt records support canonical proline-biosynthesis ProB and ProA functions.
GO:0055129
L-proline biosynthetic process
GO:0055129 captures the proline-biosynthetic context for ProB and ProA.
file:PSEPK/proB/proB-uniprot.txt
UniProtKB entry for PSEPK proB
Q88Q07 is a reviewed glutamate 5-kinase and step 1/2 of L-glutamate-5-semialdehyde formation from L-glutamate.
file:PSEPK/proA/proA-uniprot.txt
UniProtKB entry for PSEPK proA
Q88DL4 is a reviewed gamma-glutamyl phosphate reductase and step 2/2 of L-glutamate-5-semialdehyde formation from L-glutamate.

First-pass PSEPK interpretation: proB and proA satisfy the glutamate-to-P5C branch but not the complete L-proline biosynthesis pathway, which also needs a P5C reductase/ProC-type terminal step. Keep the misleading KEGG carbapenem-biosynthesis label out of the module biology.

3Nodes
2Parts
0Variant Sets
0Variants
2Annotons
1Connections

Derived QC

Recommended-field compliance

60.0% recommended fields populated
  • module.knowledge_gaps[0] · status (0/1)
  • module.knowledge_gaps[0] · provenance (0/1)

Module deep research

✓ present

  • bacterial_glutamate_to_p5c_biosynthesis-deep-research-openscientist.md (openscientist)

Leaf nodes lacking representative members

✓ every leaf node grounds to a representative protein.

Template conformance

✓ every declared conforms_to bundle matches its template motif.

Gene-review completeness (2/2 grounded genes reviewed)

2 complete review(s) · 0 with deep research · 0 missing review · 2 reviewed but lacking deep research

Gene Review Complete Deep research
proA Q88DL4 ✓ ✓ ✗
proB Q88Q07 ✓ ✓ ✗

Details

Context
bacteriaNCBITaxon:2
cytoplasmGO:0005737
Bacterial glutamate-to-P5C branch of proline biosynthesisMetabolic Pathwaybacterial_glutamate_to_p5c_biosynthesis
L-proline biosynthetic processGO:0055129
Context
bacteriaNCBITaxon:2
cytoplasmGO:0005737

Connections

ProB produces L-glutamyl 5-phosphate, the substrate reduced by ProA to L-glutamate 5-semialdehyde/P5C.
Part 1: glutamate phosphorylation
ProB glutamate 5-kinaseReactionprob_glutamate_5_kinase_step

Annotons

proB: glutamate 5-kinase
prob_glutamate_5_kinase
Participant: Family: bacterial glutamate 5-kinase family
Family:
bacterial glutamate 5-kinase familyPANTHER:PTHR43654
Representative Members: PSEPK proB exemplarUniProtKB:Q88Q07

Function

glutamate 5-kinase activityGO:0004349
Substrates: L-glutamate ATP
Products: L-glutamyl 5-phosphate ADP

Processes

L-proline biosynthetic processGO:0055129

Locations

cytoplasmGO:0005737

First committed step that activates L-glutamate as L-glutamyl 5-phosphate.

Part 2: gamma-glutamyl phosphate reduction to P5C precursor
ProA gamma-glutamyl phosphate reductaseReactionproa_gamma_glutamyl_phosphate_reductase_step

Annotons

proA: gamma-glutamyl phosphate reductase
proa_glutamate_5_semialdehyde_dehydrogenase
Participant: Family: bacterial gamma-glutamyl phosphate reductase family
Family:
bacterial gamma-glutamyl phosphate reductase familyPANTHER:PTHR11063
Representative Members: PSEPK proA exemplarUniProtKB:Q88DL4

Function

glutamate-5-semialdehyde dehydrogenase activityGO:0004350
Substrates: L-glutamyl 5-phosphate NADPH
Products: L-glutamate 5-semialdehyde phosphate NADP+

Processes

L-proline biosynthetic processGO:0055129

Locations

cytoplasmGO:0005737

Reduces the ProB product to L-glutamate 5-semialdehyde, which can cyclize to P5C.