Bacterial Lol-dependent outer-membrane lipoprotein trafficking

A species-neutral diderm-bacterial module for ATP-dependent release of outer-membrane-destined mature lipoproteins from the inner membrane by LolCDE, periplasmic carriage by LolA, and delivery into the outer membrane by LolB. Lipoprotein maturation and inner-membrane retention decisions are upstream of this module.

MODULE:bacterial_lol_lipoprotein_traffickingDRAFTBiological Processmodules/bacterial_lol_lipoprotein_trafficking.yaml
lipoprotein localization to outer membraneGO:0044874
GO:0044874
lipoprotein localization to outer membrane
GO:0044874 captures the shared biological-process outcome of the three-stage Lol pathway.
file:PSEPK/lolA/lolA-uniprot.txt
PSEPK LolA UniProt record
Q88FS9 is the periplasmic carrier between LolCDE and LolB.
file:PSEPK/lolB/lolB-uniprot.txt
PSEPK LolB UniProt record
Q88PX4 incorporates LolA-delivered lipoproteins into the outer membrane.
file:PSEPK/lolD/lolD-uniprot.txt
PSEPK LolD UniProt record
Q88KY4 is the ATPase subunit of the LolCDE release complex.
file:modules/bacterial_lol_lipoprotein_trafficking-deep-research-openscientist.md
OpenScientist research for the reusable Lol trafficking module
The report supports the post-maturation three-stage boundary, confines ATP use to LolCDE extraction, distinguishes mechanotransmission from transmembrane pore transport, and identifies lineage variation in extractor and outer-membrane-delivery architecture.
file:projects/P_PUTIDA/deep-research/PSEPK__bacterial_lol_lipoprotein_trafficking__ppu02010-deep-research-openscientist.md
OpenScientist PSEPK Lol-pathway satisfiability review
The report independently identifies lolC/PP_2154, lolD/PP_2155, lolE/PP_2156, lolA/PP_4003, and lolB/PP_0724 as the complete canonical KT2440 system and finds no biological gap.

Reusable boundary: this module begins with extraction of an already mature outer-membrane-destined lipoprotein and ends with outer-membrane delivery. Lgt/LspA/Lnt maturation is upstream and substrate-specific lipoprotein function is downstream. Pseudomonas putida KT2440 provides exemplars for all five canonical Lol components. The modeled three-part relay is the canonical LolCDE-LolA-LolB implementation, not a claim that every diderm lineage has LolB or a heterodimeric LolC/LolE extractor.

4Nodes
3Parts
0Variant Sets
0Variants
3Annotons
2Connections

Derived QC

Recommended-field compliance

52.9% recommended fields populated
  • module.knowledge_gaps[0] · status (0/1)
  • module.knowledge_gaps[0] · provenance (0/1)
  • module.knowledge_gaps[1] · status (0/1)
  • module.knowledge_gaps[1] · provenance (0/1)
  • module.knowledge_gaps[2] · status (0/1)
  • module.knowledge_gaps[2] · provenance (0/1)
  • module.knowledge_gaps[3] · status (0/1)
  • module.knowledge_gaps[3] · provenance (0/1)

Module deep research

✓ present

  • bacterial_lol_lipoprotein_trafficking-deep-research-openscientist.md (openscientist)

Leaf nodes lacking representative members

✓ every leaf node grounds to a representative protein.

Template conformance

✓ every declared conforms_to bundle matches its template motif.

Gene-review completeness (5/5 grounded genes reviewed)

5 complete review(s) · 4 with deep research · 0 missing review · 1 reviewed but lacking deep research

Gene Review Complete Deep research
lolA Q88FS9 ✓ ✓ ✗
lolB Q88PX4 ✓ ✓ ✓
lolC Q88KY5 ✓ ✓ ✓
lolD Q88KY4 ✓ ✓ ✓
lolE Q88KY3 ✓ ✓ ✓

Details

Context
diderm bacteriaNCBITaxon:2
Bacterial Lol-dependent outer-membrane lipoprotein traffickingBiological Processbacterial_lol_lipoprotein_trafficking
lipoprotein localization to outer membraneGO:0044874
Context
diderm bacteriaNCBITaxon:2

Connections

LolCDE loads released lipoprotein onto LolA.
lola_carriage_step -> lolb_delivery_step Provides Input For
LolA presents its lipoprotein cargo to LolB.
Part 1: ATP-dependent inner-membrane extraction and release
LolCDE lipoprotein releaseTransport Steplolcde_release_step

LolCDE uses ATP hydrolysis to extract an outer-membrane-destined mature lipoprotein from the inner membrane and load it onto LolA.

Annotons

LolCDE lipoprotein-release complex
lolcde_release_complex
Participant: Protein Complex: LolCDE ABC lipoprotein-release complex
Protein Complex:
LolCDE ABC lipoprotein-release complex Heteromeric inner-membrane complex with LolC, LolD, and LolE subunits.
Active units:
LolC membrane subunit
Participant: Family: LolC family
Family:
LolC family
Representative Members: PSEPK LolC exemplarUniProtKB:Q88KY5
Role: Integral membrane subunit contributing to lipoprotein extraction.
LolD ATPase subunit
Participant: Family: LolD family
Family:
LolD familyPANTHER:PTHR24220:SF689
Representative Members: PSEPK LolD exemplarUniProtKB:Q88KY4
Role: Cytoplasmic ATPase that powers lipoprotein extraction.
Function:
ATP hydrolysis activityGO:0016887
LolE membrane subunit
Participant: Family: LolE family
Family:
LolE family
Representative Members: PSEPK LolE exemplarUniProtKB:Q88KY3
Role: Integral membrane subunit contributing to lipoprotein extraction. LolC/E sequence classifications may not distinguish this role from LolC, so concrete assignments require orthology and genomic context.

Function

ATP-dependent lipoprotein extraction and release activity

Processes

lipoprotein transportGO:0042953 lipoprotein localization to outer membraneGO:0044874

Locations

plasma membraneGO:0005886

Initiates trafficking by releasing the mature lipoprotein from the inner membrane to LolA.

Part 2: Periplasmic lipoprotein carriage
LolA periplasmic carriageTransport Steplola_carriage_step

LolA shields the lipid anchor while carrying the released lipoprotein across the periplasm.

Annotons

LolA periplasmic lipoprotein carrier
lola_carrier
Participant: Family: LolA family
Family:
LolA familyPANTHER:PTHR35869:SF1
Representative Members: PSEPK LolA exemplarUniProtKB:Q88FS9

Function

periplasmic lipoprotein carrier activity

Processes

lipoprotein transportGO:0042953 lipoprotein localization to outer membraneGO:0044874

Locations

outer membrane-bounded periplasmic spaceGO:0030288

Accepts lipoprotein from LolCDE and presents it to LolB.

Part 3: Outer-membrane delivery and incorporation
LolB outer-membrane deliveryTransport Steplolb_delivery_step

LolB receives the LolA-bound lipoprotein and promotes its incorporation into the outer membrane.

Annotons

LolB outer-membrane lipoprotein receptor
lolb_receptor
Participant: Family: LolB family
Family:
LolB family
Representative Members: PSEPK LolB exemplarUniProtKB:Q88PX4

Function

outer-membrane lipoprotein delivery activity

Processes

lipoprotein transportGO:0042953 lipoprotein localization to outer membraneGO:0044874

Locations

cell outer membraneGO:0009279

Terminal pathway step that incorporates the delivered lipoprotein into the outer membrane.