E. coli ManXYZ mannose-family PTS

A bacterial phosphoenolpyruvate-dependent group-translocation module in which the soluble/peripheral ManX EIIAB subunit relays phosphate from HPr through its EIIA and EIIB histidines to sugar entering through the inner-membrane ManY/ManZ channel. ManY and ManZ form the EIIC/EIID membrane translocation subcomplex, while all three subunits are required for efficient transport and concomitant phosphorylation of mannose-family substrates. Upstream enzyme I and HPr, dedicated fructose PTS systems, nitrogen-regulatory PTS signaling, sugar chemotaxis, and bacteriophage lambda DNA penetration are neighboring processes outside this transport module.

MODULE:bacterial_mannose_ptsDRAFTCONCRETETransport Stepmodules/bacterial_mannose_pts.yaml
phosphoenolpyruvate-dependent sugar phosphotransferase systemGO:0009401 mannose transmembrane transportGO:0015761
GO:0009401
phosphoenolpyruvate-dependent sugar phosphotransferase system
Defines the PEP-dependent sugar group-translocation process.
GO:0015761
mannose transmembrane transport
Defines the core mannose import process catalyzed by ManXYZ.
GO:0022870
protein-N(PI)-phosphohistidine-mannose phosphotransferase system transporter activity
Defines the ManXYZ complex-level transport and phosphorylation activity.
file:genes/ECOLI/manX/manX-ai-review.yaml
ECOLI manX gene review
The completed manX review grounds ManX as the EIIAB phosphorylating subunit of the E. coli mannose-specific PTS.
file:genes/ECOLI/manY/manY-ai-review.yaml
ECOLI manY gene review
The completed manY review grounds ManY as the EIIC channel component of the ManXYZ inner-membrane translocation subcomplex.
file:genes/ECOLI/manZ/manZ-ai-review.yaml
ECOLI manZ gene review
The completed manZ review grounds ManZ as the EIID partner of ManY in the ManXYZ inner-membrane translocation subcomplex.
PMID:2951378
The mannose permease of Escherichia coli consists of three different proteins. Amino acid sequence and function in sugar transport, sugar phosphorylation, and penetration of phage lambda DNA.
Establishes the IIIMan/ManX, II-PMan/ManY, and II-MMan/ManZ composition of E. coli mannose permease and the requirement for all three subunits in sugar transport and phosphorylation.
PMID:2999119
The mannose-permease of the bacterial phosphotransferase system. Gene cloning and purification of the enzyme IIMan/IIIMan complex of Escherichia coli.
Establishes IIMan/IIIMan complex purification, PEP-dependent phosphorylation of IIIMan/ManX, and the requirement for membrane IIMan and soluble ManX in 2-deoxyglucose phosphorylation.
PMID:2681202
Mannose permease of Escherichia coli. Domain structure and function of the phosphorylating subunit.
Establishes the ManX EIIA and EIIB domain architecture and their distinct histidyl phosphorylation sites.
PMID:8262947
The mannose transporter of Escherichia coli. Structure and function of the IIABMan subunit.
Mutational analysis supports the catalytic importance of the ManX EIIA and EIIB histidines.
PMID:31209249
Structure of the mannose transporter of the bacterial phosphotransferase system.
Cryo-EM of E. coli ManYZ establishes the ManY-ManZ membrane architecture.

ManXYZ is broad-substrate relative to many sugar PTS transporters: mannose is the named core substrate, but the same EII complex also imports and phosphorylates 2-deoxyglucose and several other hexoses or amino sugars. This module therefore represents the ManXYZ EII apparatus rather than trying to partition every secondary sugar into a separate route. Phage lambda DNA penetration and sugar chemotaxis use the ManYZ membrane subcomplex but are separate biological outputs.

3Nodes
2Parts
0Variant Sets
0Variants
2Annotons
1Connections

Derived QC

Recommended-field compliance

100.0% recommended fields populated

All recommended fields populated.

Module deep research

✗ none found

No MODULE:bacterial_mannose_pts deep-research report alongside the module YAML.

Leaf nodes lacking representative members

✓ every leaf node grounds to a representative protein.

Template conformance

✓ every declared conforms_to bundle matches its template motif.

Function consistency and core coverage

0 gene-function check(s) reflected in core functions · 0 conflict(s) · 1 evidence or coverage gap(s) (2 comparison(s))

Retained noncore functions are reported separately; they do not require promotion to core functions. Support from representative genes does not establish conservation across the whole family. Missing reviews or identifiers leave a claim unverified.

AnnotonParticipantFunctionAssessment
manx_internal_relay_activity E. coli ManX
UniProtKB:P69797
Gene 		intramolecular ManX EIIA-to-EIIB phosphorelay
No function ID 		No Function Id
A gene review exists, but this function lacks a GO identifier. Free-text chemistry is not automatically compared.
manxyz_mannose_pts_activity PROTEIN_COMPLEX
Gene 		protein-N(PI)-phosphohistidine-mannose phosphotransferase system transporter activity
GO:0022870 		Participant Unchecked
No direct UniProt participant to compare. Abstract selectors and complex-wide activities cannot be inferred from donor or subunit gene reviews.

Gene-review completeness (3/3 grounded genes reviewed)

3 complete review(s) · 3 with deep research · 0 missing review · 0 reviewed but lacking deep research

Gene Review Complete Deep research
manX P69797 ✓ ✓ ✓
manY P69801 ✓ ✓ ✓
manZ P69805 ✓ ✓ ✓

Details

Context
Escherichia coliNCBITaxon:562
E. coli ManXYZ mannose-family PTSTransport Stepbacterial_mannose_pts
phosphoenolpyruvate-dependent sugar phosphotransferase systemGO:0009401 mannose transmembrane transportGO:0015761
Context
Escherichia coliNCBITaxon:562

Connections

Phosphorylated ManX EIIB His-175 donates phosphate to incoming D-mannose.
Part 1: ManX internal EIIA-to-EIIB phosphorelay
ManX His-10 to His-175 phosphorelayReactionmanx_internal_phosphorelay

The ManX EIIAB subunit relays phosphate from its EIIA His-10 site to the EIIB His-175 site that donates phosphate to incoming mannose-family sugars.

Annotons

ManX internal EIIA-to-EIIB relay
manx_internal_relay_activity
Participant: Gene Product: E. coli ManX
Gene Product:
E. coli ManXUniProtKB:P69797

Function

intramolecular ManX EIIA-to-EIIB phosphorelay
Substrates: phosphorylated ManX EIIA His-10 unphosphorylated ManX EIIB His-175
Products: unphosphorylated ManX EIIA His-10 phosphorylated ManX EIIB His-175

Processes

phosphoenolpyruvate-dependent sugar phosphotransferase systemGO:0009401

Locations

cytosolGO:0005829 plasma membraneGO:0005886

His-10 in the ManX EIIA domain receives phosphate from HPr and His-175 in the EIIB domain receives it next, priming the terminal phosphoryl transfer to the transported sugar.

Part 2: ManYZ-coupled mannose transport and phosphorylation
ManXYZ transport and mannose phosphorylationTransport Stepmanxyz_transport_phosphorylation

ManY and ManZ form the inner-membrane translocation subcomplex, while the phosphorylated ManX EIIB domain donates phosphate to the sugar as it enters the cytoplasm.

Annotons

ManXYZ mannose-family PTS activity
manxyz_mannose_pts_activity
Participant: Protein Complex: E. coli ManXYZ EII complex
Protein Complex:
E. coli ManXYZ EII complexGO:1902495
Active units:
ManX EIIAB phosphorelay subunit
Participant: Gene Product: E. coli ManX
Gene Product:
E. coli ManXUniProtKB:P69797
Role: Cytosolic/peripheral EIIAB subunit whose His-175 donates phosphate to the incoming sugar.
ManY EIIC translocation subunit
Participant: Gene Product: E. coli ManY
Gene Product:
E. coli ManYUniProtKB:P69801
Role: Integral membrane EIIC subunit that partners with ManZ to form the sugar-translocation channel.
ManZ EIID translocation subunit
Participant: Gene Product: E. coli ManZ
Gene Product:
E. coli ManZUniProtKB:P69805
Role: Integral membrane EIID subunit that partners with ManY in the translocation subcomplex.

Function

protein-N(PI)-phosphohistidine-mannose phosphotransferase system transporter activityGO:0022870
Substrates: extracellular D-mannose phosphorylated ManX EIIB His-175
Products: intracellular D-mannose 6-phosphate unphosphorylated ManX

Processes

phosphoenolpyruvate-dependent sugar phosphotransferase systemGO:0009401 mannose transmembrane transportGO:0015761

Locations

plasma membraneGO:0005886

ManY and ManZ form the membrane channel that positions incoming mannose-family sugars for phosphorylation by the ManX EIIB domain.