A Tus-dependent bacterial sulfur-relay module that mobilizes sulfur from cysteine and passes it through TusA, the TusBCD complex, and TusE to MnmA, which installs 2-thiouridine at the wobble uridine of substrate tRNAs. Sulfur mobilization, carrier handoffs, complex-mediated transfer, and tRNA thiolation are modeled as separate substantive roles.
This module represents the Tus-dependent implementation of the MnmA pathway; it is not asserted to be universal across bacteria. IscS also supplies sulfur to iron-sulfur cluster and cofactor pathways; those branches are outside this boundary. MnmC-dependent elaboration of the modified uridine is downstream and is likewise excluded. The exact KT2440 gene names for Q88FT8, Q88FT7, and Q88FT6 remain locus-tag based in the review files, while their family roles are represented as TusC, TusB, and TusE here.
All recommended fields populated.
✗ none found
No MODULE:bacterial_mnma_trna_thiolation deep-research report alongside the module YAML.
✓ every leaf node grounds to a representative protein.
✓ every declared conforms_to bundle matches its template motif.
5 gene-function check(s) reflected in core functions · 0 conflict(s) · 5 evidence or coverage gap(s) (10 comparison(s))
Retained noncore functions are reported separately; they do not require promotion to core functions. Support from representative genes does not establish conservation across the whole family. Missing reviews or identifiers leave a claim unverified.
| Annoton | Participant | Function | Assessment |
|---|---|---|---|
| iscs_cysteine_desulfurase | PSEPK IscS exemplar UniProtKB:Q88PK8 Family Representative |
cysteine desulfurase activity GO:0031071 |
Core Supported
The GO molecular function is reflected in core_functions (exact term or a reviewed subclass). |
| tusa_sulfur_carrier | PSEPK TusA exemplar UniProtKB:Q88L21 Family Representative |
sulfur carrier activity GO:0097163 |
Core Supported
The GO molecular function is reflected in core_functions (exact term or a reviewed subclass). |
| tusd_sulfur_carrier | PSEPK TusD exemplar UniProtKB:Q88FT9 Family Representative |
sulfur carrier activity GO:0097163 |
Core Supported
The GO molecular function is reflected in core_functions (exact term or a reviewed subclass). |
| tuse_sulfur_carrier | PSEPK TusE exemplar UniProtKB:Q88FT6 Family Representative |
sulfur carrier activity GO:0097163 |
Core Supported
The GO molecular function is reflected in core_functions (exact term or a reviewed subclass). |
| mnma_trna_uridine_sulfurtransferase | PSEPK MnmA exemplar UniProtKB:Q88FR9 Family Representative |
tRNA-uridine 2-sulfurtransferase activity GO:0103016 |
Core Supported
The GO molecular function is reflected in core_functions (exact term or a reviewed subclass). |
| iscs_cysteine_desulfurase | IscS family InterPro:IPR010240 Family |
cysteine desulfurase activity GO:0031071 |
Family Review Missing
No supported structured family review is available for InterPro:IPR010240. |
| tusa_sulfur_carrier | TusA family InterPro:IPR022931 Family |
sulfur carrier activity GO:0097163 |
Family Review Missing
No supported structured family review is available for InterPro:IPR022931. |
| tusd_sulfur_carrier | TusD family PANTHER:PTHR34874 Family |
sulfur carrier activity GO:0097163 |
Family Review Missing
No supported structured family review is available for PANTHER:PTHR34874. |
| tuse_sulfur_carrier | TusE family PANTHER:PTHR37010 Family |
sulfur carrier activity GO:0097163 |
Family Review Missing
No supported structured family review is available for PANTHER:PTHR37010. |
| mnma_trna_uridine_sulfurtransferase | MnmA family InterPro:IPR004506 Family |
tRNA-uridine 2-sulfurtransferase activity GO:0103016 |
Family Review Missing
No supported structured family review is available for InterPro:IPR004506. |
6 complete review(s) · 1 with deep research · 0 missing review · 6 reviewed but lacking deep research
| Gene | Review | Complete | Deep research |
|---|---|---|---|
| iscS Q88PK8 | ✓ | 5/6 | ✓ |
| mnmA Q88FR9 | ✓ | ✓ | ✗ |
| PP_3994 Q88FT8 | ✓ | ✓ | ✗ |
| PP_3995 Q88FT7 | ✓ | ✓ | ✗ |
| tusA Q88L21 | ✓ | ✓ | ✗ |
| tusD Q88FT9 | ✓ | ✓ | ✗ |
| tusE Q88FT6 | ✓ | ✓ | ✗ |
A cysteine desulfurase generates enzyme-bound persulfide sulfur that enters the Tus relay.
Mobilizes sulfur from cysteine for entry into the relay.
TusA receives mobilized sulfur and transfers it to TusBCD.
Carries sulfur from IscS to the TusBCD complex.
TusB, TusC, and TusD form a heterohexameric sulfur-transfer complex; TusD carries the transferable sulfur while all three subunits are required for the organized relay.
Sulfur-bearing TusBCD subunit.
Structural TusBCD subunit required for relay-complex assembly.
Structural TusBCD subunit required for relay-complex assembly.
TusE carries sulfur from TusBCD to MnmA.
Terminal soluble sulfur carrier that supplies MnmA.
MnmA activates wobble uridine using ATP and incorporates relay-derived sulfur to form 2-thiouridine.
Catalyzes the terminal ATP-dependent tRNA thiolation reaction.