Bacterial nitrogen-regulatory PTS phosphorelay

A species-neutral bacterial signaling module in which enzyme I(Ntr) accepts phosphate from phosphoenolpyruvate, NPr relays it, and EIIA(Ntr) acts as the terminal phosphorylation-state-dependent regulator. Unlike carbohydrate PTS pathways, this module does not transport or phosphorylate a sugar.

MODULE:bacterial_nitrogen_regulatory_ptsDRAFTCONCRETEBiological Processmodules/bacterial_nitrogen_regulatory_pts.yaml
protein phosphorylationGO:0006468
PMID:17416664
The phosphotransferase system formed by PtsP, PtsO, and PtsN proteins controls production of polyhydroxyalkanoates in Pseudomonas putida
Genetic analysis identifies PtsP, PtsO/NPr, and PtsN as a regulatory phosphotransfer system controlling PHA accumulation in KT2440.
PMID:18296519
Evidence of in vivo cross talk between the nitrogen-related and fructose-related branches of the carbohydrate phosphotransferase system of Pseudomonas putida
In vivo phosphorylation-state analysis establishes the primary flow as PEP to PtsP to PtsO/NPr to PtsN and separates this branch from sugar uptake, while documenting conditional FruB cross-talk.
PMID:26224366
Interplay of the PtsN (EIIA(Ntr)) protein of Pseudomonas putida with its target sensor kinase KdpD
KT2440 PtsN directly interacts with KdpD and controls kdpFABC expression in a phosphorylation-state- and potassium-dependent manner.
PMID:21236318
The interplay of the EIIA(Ntr) component of the nitrogen-related phosphotransferase system (PTS(Ntr)) of Pseudomonas putida with pyruvate dehydrogenase
Non-phosphorylated KT2440 PtsN directly interacts with AceE and inhibits pyruvate dehydrogenase activity.
PMID:19400808
Stimulation of the potassium sensor KdpD kinase activity by interaction with the phosphotransferase protein IIA(Ntr) in Escherichia coli
E. coli EI(Ntr), NPr, and IIA(Ntr) form a regulatory phosphotransfer chain parallel to the carbohydrate-transport PTS, supporting the cross-species module boundary.
file:PSEPK/ptsP/ptsP-uniprot.txt
PSEPK PtsP UniProt record
Q88CN5 contains GAF, EI(Ntr), and PEP-utilizer domains.
file:PSEPK/ptsH/ptsH-uniprot.txt
PSEPK NPr/PtsH UniProt record
Q88PA2 is the phosphocarrier experimentally assigned as NPr/PtsO between PtsP and PtsN in KT2440.
file:PSEPK/ptsN/ptsN-uniprot.txt
PSEPK PtsN UniProt record
Q88PA0 carries the nitrogen-regulatory EIIA domain.

In the current UniProt record, KT2440 NPr is named ptsH (PP_0948, Q88PA2), whereas the primary literature calls the same functional role ptsO/NPr. FruB can feed phosphate into NPr/PtsN during fructose growth, but this experimentally demonstrated cross-talk is an alternative input rather than a required part of the nitrogen-regulatory PTS. The conserved PtsN-KdpD kinase regulatory interaction is represented on the terminal annoton, but the Kdp transport apparatus itself, PHA control, AceE regulation, and other lineage-specific outputs are not required module parts. E. coli K-12 proteins are included as cross-species exemplars of each conserved relay role. PANTHER:PTHR33705:SF2 is not used as the NPr selector because its local member set also contains canonical carbohydrate-PTS HPr proteins.

4Nodes
3Parts
0Variant Sets
0Variants
3Annotons
2Connections

Derived QC

Recommended-field compliance

53.8% recommended fields populated
  • module.knowledge_gaps[0] · status (0/1)
  • module.knowledge_gaps[0] · provenance (0/1)
  • module.knowledge_gaps[1] · status (0/1)
  • module.knowledge_gaps[1] · provenance (0/1)
  • module.knowledge_gaps[2] · status (0/1)
  • module.knowledge_gaps[2] · provenance (0/1)

Module deep research

✗ none found

No MODULE:bacterial_nitrogen_regulatory_pts deep-research report alongside the module YAML.

Leaf nodes lacking representative members

✓ every leaf node grounds to a representative protein.

Template conformance

✓ every declared conforms_to bundle matches its template motif.

Gene-review completeness (3/6 grounded genes reviewed)

3 complete review(s) · 1 with deep research · 3 missing review · 2 reviewed but lacking deep research

Gene Review Complete Deep research
E. coli K-12 NPr exemplar P0A9N0 ✗ — —
E. coli K-12 PtsP exemplar P37177 ✗ — —
E. coli K-12 PtsN exemplar P69829 ✗ — —
ptsH Q88PA2 ✓ ✓ ✗
ptsN Q88PA0 ✓ ✓ ✓
ptsP Q88CN5 ✓ ✓ ✗

Details

Context
bacteriaNCBITaxon:2
Bacterial nitrogen-regulatory PTS phosphorelayBiological Processbacterial_nitrogen_regulatory_pts
protein phosphorylationGO:0006468
Context
bacteriaNCBITaxon:2

Connections

Part 1: PEP-dependent phosphorylation entry
PtsP enzyme I(Ntr) phosphate entryReactionptsp_phosphate_entry

PtsP accepts phosphate from phosphoenolpyruvate on its EI histidine and transfers it to the NPr phosphocarrier.

Annotons

PtsP enzyme I(Ntr)
ptsp_enzyme_i_ntr
Participant: Family: PtsP enzyme I(Ntr) family
Family:
PtsP enzyme I(Ntr) familyPANTHER:PTHR46244:SF1
Representative Members: E. coli K-12 PtsP exemplarUniProtKB:P37177 PSEPK PtsP exemplarUniProtKB:Q88CN5

Function

phosphoenolpyruvate-protein phosphotransferase activityGO:0008965

Entry enzyme that couples PEP dephosphorylation to phosphorylation of the soluble NPr carrier.

Part 2: NPr phosphocarrier relay
NPr phosphocarrier relayReactionnpr_phosphocarrier_relay

NPr receives phosphate from PtsP and transfers it to EIIA(Ntr).

Annotons

NPr phosphocarrier
npr_phosphocarrier
Participant: Family: nitrogen-regulatory NPr phosphocarrier family
Family:
nitrogen-regulatory NPr phosphocarrier family NPr proteins assigned by pathway context to the carrier step between EI(Ntr) and EIIA(Ntr). No PANTHER term is asserted because the available subfamily also includes canonical carbohydrate-PTS HPr proteins.
Representative Members: E. coli K-12 NPr exemplarUniProtKB:P0A9N0 PSEPK NPr exemplarUniProtKB:Q88PA2

Function

transferase activity, transferring phosphorus-containing groupsGO:0016772

Soluble intermediate carrier between PtsP and PtsN; the broad GO function is used because GO lacks a specific NPr phosphocarrier molecular-function term.

Part 3: Terminal EIIA(Ntr) phosphorylation-state regulator
PtsN EIIA(Ntr) terminal regulatorReactionptsn_terminal_regulator

PtsN receives phosphate from NPr; its phosphorylation state controls interactions with downstream metabolic and transport targets.

Annotons

PtsN EIIA(Ntr)
ptsn_eiia_ntr
Participant: Family: nitrogen-regulatory EIIA family
Family:
nitrogen-regulatory EIIA familyPANTHER:PTHR47738:SF1
Representative Members: E. coli K-12 PtsN exemplarUniProtKB:P69829 PSEPK PtsN exemplarUniProtKB:Q88PA0

Function

protein kinase regulator activityGO:0019887

Processes

intracellular signal transductionGO:0035556 regulation of potassium ion transmembrane transportGO:1901379

Terminal phosphorelay component and phosphorylation-state-dependent regulatory effector. KdpD regulation is supported in both P. putida and E. coli, although the direction differs; AceE and PHA outputs remain lineage-specific extensions of the conserved relay.