E. coli periplasmic organophosphate scavenging

A phosphate-starvation effector module in which E. coli periplasmic phosphatases liberate inorganic phosphate from extracellular-facing organophosphate substrates. PhoA hydrolyzes phosphate monoesters through its alkaline phosphatase activity, while AppA contributes a phytase/acid phosphatase branch under phosphate limitation. Sec export, PhoB/PhoR transcriptional induction, and Pst phosphate import are adjacent processes outside this mature periplasmic scavenging boundary.

MODULE:bacterial_periplasmic_phosphate_scavengingCOMPLETECONCRETEBiological Processmodules/bacterial_periplasmic_phosphate_scavenging.yaml
dephosphorylationGO:0016311
GO:0016311
dephosphorylation
Defines phosphate-group removal as the shared process represented by both hydrolase branches.
file:genes/ECOLI/phoA/phoA-ai-review.yaml
ECOLI phoA gene review
The completed E. coli phoA review grounds PhoA as the periplasmic alkaline phosphatase used for phosphate scavenging.
file:genes/ECOLI/appA/appA-ai-review.yaml
ECOLI appA gene review
The completed E. coli appA review grounds AppA as the periplasmic histidine acid phosphatase/phytase used for phosphate scavenging, with GTPase and nucleotidase rows marked over-annotated and generic sugar-phosphatase activity retained as a non-core biochemical capability.
PMID:8387749
Purification and characterization of two phytases from Escherichia coli.
Purifies E. coli periplasmic phytases and identifies the P2 phytase with the pH 2.5 acid phosphatase described by Dassa and colleagues.
PMID:11035187
Stereospecificity of myo-inositol hexakisphosphate dephosphorylation by a phytate-degrading enzyme of Escherichia coli.
Shows that the E. coli P2 phytate-degrading enzyme dephosphorylates myo-inositol hexakisphosphate stepwise.
PMID:13826559
A fine-structure genetic and chemical study of the enzyme alkaline phosphatase of E. coli. I. Purification and characterization of alkaline phosphatase.
Establishes the foundational purification and characterization of E. coli PhoA.
PMID:14487220
Alkaline phosphatase of Escherichia coli: a zinc metalloenzyme.
Establishes PhoA as a zinc metalloenzyme.
PMID:6282821
The acid phosphatase with optimum pH of 2.5 of Escherichia coli. Physiological and Biochemical study.
Establishes AppA as an acid phosphatase and phosphoanhydride phosphatase whose synthesis is prematurely fully induced by inorganic phosphate starvation.

PhoA also oxidizes phosphite slowly in vitro, but phosphate monoester hydrolysis is its physiological phosphate-scavenging activity. GO:0016036 cellular response to phosphate starvation is a valid process term for the effector genes, but is not listed as a module concept because the term also covers PhoB/PhoR transcriptional regulation, which is outside this boundary.

3Nodes
2Parts
0Variant Sets
0Variants
2Annotons
0Connections

Derived QC

Recommended-field compliance

100.0% recommended fields populated

All recommended fields populated.

Module deep research

✗ none found

No MODULE:bacterial_periplasmic_phosphate_scavenging deep-research report alongside the module YAML.

Leaf nodes lacking representative members

✓ every leaf node grounds to a representative protein.

Template conformance

✓ every declared conforms_to bundle matches its template motif.

Function consistency and core coverage

2 gene-function check(s) reflected in core functions · 0 conflict(s) · 0 evidence or coverage gap(s) (2 comparison(s))

Retained noncore functions are reported separately; they do not require promotion to core functions. Support from representative genes does not establish conservation across the whole family. Missing reviews or identifiers leave a claim unverified.

AnnotonParticipantFunctionAssessment
phoa_alkaline_phosphatase E. coli PhoA
UniProtKB:P00634
Gene 		alkaline phosphatase activity
GO:0004035 		Core Supported
The GO molecular function is reflected in core_functions (exact term or a reviewed subclass).
appa_phytase E. coli AppA
UniProtKB:P07102
Gene 		inositol phosphate phosphatase activity
GO:0052745 		Core Supported
The GO molecular function is reflected in core_functions (exact term or a reviewed subclass).

Gene-review completeness (2/2 grounded genes reviewed)

1 complete review(s) · 2 with deep research · 0 missing review · 0 reviewed but lacking deep research

Gene Review Complete Deep research
appA P07102 ✓ ✓ ✓
phoA P00634 ✓ 20/22 ✓

Details

Context
Escherichia coli K-12NCBITaxon:83333
E. coli periplasmic organophosphate scavengingBiological Processbacterial_periplasmic_phosphate_scavenging
dephosphorylationGO:0016311
Context
Escherichia coli K-12NCBITaxon:83333
Part 1: alkaline phosphate-monoester hydrolysis
PhoA alkaline phosphatase branchReactionphoa_phosphate_monoester_hydrolysis

Annotons

PhoA alkaline phosphatase
phoa_alkaline_phosphatase
Participant: Gene Product: E. coli PhoA
Gene Product:
E. coli PhoAUniProtKB:P00634

Function

alkaline phosphatase activityGO:0004035
Substrates: phosphate monoester water
Products: alcohol inorganic phosphate
Cofactors: zinc ion magnesium ion

Processes

cellular response to phosphate starvationGO:0016036

Locations

outer membrane-bounded periplasmic spaceGO:0030288

Periplasmic alkaline phosphatase that hydrolyzes diverse organic phosphomonoesters and releases Pi under phosphate limitation.

Part 2: AppA acid phosphatase and phytase hydrolysis
AppA acid phosphatase branchReactionappa_phytate_and_phosphoanhydride_hydrolysis

Annotons

AppA phytase and acid phosphatase
appa_phytase
Participant: Gene Product: E. coli AppA
Gene Product:
E. coli AppAUniProtKB:P07102

Function

inositol phosphate phosphatase activityGO:0052745
Substrates: myo-inositol hexakisphosphate water
Products: less phosphorylated myo-inositol phosphates inorganic phosphate

Processes

cellular response to phosphate starvationGO:0016036

Locations

outer membrane-bounded periplasmic spaceGO:0030288

Periplasmic 6-phytase and acid phosphatase, adding a second organophosphate hydrolase branch to the phosphate-scavenging response.