Bacterial Pst phosphate uptake

A reusable bacterial high-affinity phosphate-uptake module in which PstS captures inorganic phosphate, the PstA/PstC membrane subunits form the translocation pathway, and a PstB dimer supplies ATP-dependent energy coupling. The module represents uptake by the PstSACB transporter and ends with cytoplasmic phosphate delivery. PhoU-mediated homeostasis and PhoR/PhoB starvation signaling regulate this system but are not transport steps.

MODULE:bacterial_pst_phosphate_uptakeCOMPLETECONCRETEBiological Processmodules/bacterial_pst_phosphate_uptake.yaml
phosphate ion transmembrane transportGO:0035435
GO:0035435
phosphate ion transmembrane transport
Defines movement of phosphate across the membrane by the Pst system.
GO:0015415
ATPase-coupled phosphate ion transmembrane transporter activity
Defines the collective ATP-coupled transport function of PstSACB.
UniProtKB:P0AG82
Reviewed Escherichia coli K-12 PstS
The reviewed PstS entry describes a transporter containing one solute-binding PstS, PstA and PstC membrane proteins, and two PstB ATP-binding proteins.
UniProtKB:P0AAH0
Reviewed Escherichia coli K-12 PstB
The reviewed PstB entry identifies the phosphate-import ATPase and the two-copy PstB stoichiometry of the PstSACB transporter.
file:modules/bacterial_pst_phosphate_uptake-deep-research-openscientist.md
OpenScientist research for the reusable bacterial Pst uptake module
The generic review supports the PstS capture, PstA/PstC translocation, and PstB energy-coupling boundary while keeping Pho signaling outside.
file:ECOLI/pstS/pstS-ai-review.yaml
Reviewed Escherichia coli K-12 PstS gene function
The E. coli PstS review grounds phosphate binding in the periplasm as the substrate-capture step of PstSACB.
file:ECOLI/pstA/pstA-ai-review.yaml
Reviewed Escherichia coli K-12 PstA gene function
The E. coli PstA review grounds PstA as an inner-membrane permease subunit contributing to phosphate transmembrane transporter activity.
file:ECOLI/pstC/pstC-ai-review.yaml
Reviewed Escherichia coli K-12 PstC gene function
The E. coli PstC review grounds PstC as the partner inner-membrane permease subunit contributing to phosphate transmembrane transport.
file:ECOLI/pstB/pstB-ai-review.yaml
Reviewed Escherichia coli K-12 PstB gene function
The E. coli PstB review grounds ATP hydrolysis and complex-level energy coupling as the PstB contribution to phosphate import.

Reviewed Escherichia coli K-12 proteins orient each conserved family role. Exact PANTHER subfamilies are used only where the family-specific local entries contain the representative protein. PAINT nodes are asserted only where the local IBD record supports phosphate transport by the represented family. Molecular functions occur only on leaf annotons, and no generic location is asserted. PhoU, PhoR, PhoB, low-affinity phosphate carriers, phosphonate uptake, phosphate assimilation, and downstream metabolism are outside this transport module.

4Nodes
3Parts
0Variant Sets
0Variants
3Annotons
2Connections

Derived QC

Recommended-field compliance

100.0% recommended fields populated

All recommended fields populated.

Module deep research

✓ present

  • bacterial_pst_phosphate_uptake-deep-research-openscientist.md (openscientist)

Leaf nodes lacking representative members

✓ every leaf node grounds to a representative protein.

Template conformance

✓ every declared conforms_to bundle matches its template motif.

Function consistency and core coverage

2 gene-function check(s) reflected in core functions · 0 conflict(s) · 2 evidence or coverage gap(s) (5 comparison(s))

Retained noncore functions are reported separately; they do not require promotion to core functions. Support from representative genes does not establish conservation across the whole family. Missing reviews or identifiers leave a claim unverified.

AnnotonParticipantFunctionAssessment
psts_phosphate_binding pstS (Escherichia coli K-12)
UniProtKB:P0AG82
Family Representative 		phosphate ion binding
GO:0042301 		Core Supported
The GO molecular function is reflected in core_functions (exact term or a reviewed subclass).
pstsacb_transporter_activity PROTEIN_COMPLEX
Gene 		ATPase-coupled phosphate ion transmembrane transporter activity
GO:0015415 		Participant Unchecked
No direct UniProt participant to compare. Abstract selectors and complex-wide activities cannot be inferred from donor or subunit gene reviews.
pstb_atp_hydrolysis pstB (Escherichia coli K-12)
UniProtKB:P0AAH0
Family Representative 		ATP hydrolysis activity
GO:0016887 		Core Supported
The GO molecular function is reflected in core_functions (exact term or a reviewed subclass).
psts_phosphate_binding bacterial PstS phosphate-binding proteins
PANTHER:PTHR42996:SF1
Family 		phosphate ion binding
GO:0042301 		Family Review Missing
No supported structured family review is available for PANTHER:PTHR42996:SF1.
pstb_atp_hydrolysis bacterial phosphate-import PstB ATPases
PANTHER:PTHR43423:SF3
Family 		ATP hydrolysis activity
GO:0016887 		Family Review Missing
No supported structured family review is available for PANTHER:PTHR43423:SF3.

Gene-review completeness (4/4 grounded genes reviewed)

4 complete review(s) · 0 with deep research · 0 missing review · 4 reviewed but lacking deep research

Gene Review Complete Deep research
pstA P07654 ✓ ✓ ✗
pstB P0AAH0 ✓ ✓ ✗
pstC P0AGH8 ✓ ✓ ✗
pstS P0AG82 ✓ ✓ ✗

Details

Context
bacteriaNCBITaxon:2
Bacterial Pst phosphate uptakeBiological Processbacterial_pst_phosphate_uptake
phosphate ion transmembrane transportGO:0035435
Context
bacteriaNCBITaxon:2

Connections

Phosphate-loaded PstS presents substrate to PstCAB.
PstB ATP hydrolysis drives phosphate passage through PstA/PstC.
Part 1: phosphate capture by the substrate-binding subunit
PstS phosphate captureMolecular Functionpsts_phosphate_capture

Annotons

PstS phosphate-binding activity
psts_phosphate_binding
Participant: Family: bacterial PstS phosphate-binding proteins
Family:
bacterial PstS phosphate-binding proteinsPANTHER:PTHR42996:SF1
Representative Members: pstS (Escherichia coli K-12)UniProtKB:P0AG82

Function

phosphate ion bindingGO:0042301
Substrates: extracytoplasmic inorganic phosphate
Products: PstS-bound inorganic phosphate

Captures inorganic phosphate and presents it to PstCAB.

Part 2: ATP-coupled phosphate translocation by PstSACB
PstSACB phosphate translocationProtein Complexpstsacb_phosphate_translocation

Annotons

PstSACB ATP-coupled phosphate transporter activity
pstsacb_transporter_activity
Participant: Protein Complex: bacterial PstSACB phosphate importer
Protein Complex:
bacterial PstSACB phosphate importerGO:0055052
Active units:
PstS phosphate-binding subunit1
Participant: Family: bacterial PstS phosphate-binding proteins
Family:
bacterial PstS phosphate-binding proteinsPANTHER:PTHR42996:SF1
Representative Members: pstS (Escherichia coli K-12)UniProtKB:P0AG82
Role: Captures phosphate and delivers it to the transmembrane subunits.
PstA permease subunit1
Participant: Family: bacterial PstA permease proteins
Family:
bacterial PstA permease proteinsPANTHER:PTHR42922:SF1
Representative Members: pstA (Escherichia coli K-12)UniProtKB:P07654
Role: Contributes membrane-spanning segments to the phosphate pathway.
PstC permease subunit1
Participant: Family: bacterial PstC permease proteins
Family:
bacterial PstC permease proteinsPANTHER:PTHR30425:SF1
Representative Members: pstC (Escherichia coli K-12)UniProtKB:P0AGH8
Role: Partners PstA in the membrane-spanning phosphate pathway.
PstB ATPase subunits2
Participant: Family: bacterial phosphate-import PstB ATPases
Family:
bacterial phosphate-import PstB ATPasesPANTHER:PTHR43423:SF3
Representative Members: pstB (Escherichia coli K-12)UniProtKB:P0AAH0
Role: A PstB dimer hydrolyzes ATP to energize phosphate import.

Function

ATPase-coupled phosphate ion transmembrane transporter activityGO:0015415
Substrates: extracytoplasmic inorganic phosphate ATP water
Products: cytoplasmic inorganic phosphate ADP phosphate from ATP hydrolysis

Processes

phosphate ion transmembrane transportGO:0035435

Couples PstS-dependent phosphate capture to PstA/PstC translocation and PstB ATP hydrolysis.

Part 3: ATP hydrolysis by the energy-coupling subunit
PstB ATP hydrolysisReactionpstb_energy_coupling

Annotons

PstB ATP hydrolysis activity
pstb_atp_hydrolysis
Participant: Family: bacterial phosphate-import PstB ATPases
Family:
bacterial phosphate-import PstB ATPasesPANTHER:PTHR43423:SF3
Representative Members: pstB (Escherichia coli K-12)UniProtKB:P0AAH0

Function

ATP hydrolysis activityGO:0016887
Substrates: ATP water
Products: ADP phosphate

Supplies the energy coupled to phosphate translocation.