Bacterial twin-arginine protein translocation

A species-neutral bacterial membrane-translocation module for export of folded, twin-arginine-signal-bearing proteins by the TatA, TatB, and TatC machinery. TatC and TatB form the substrate-receptor complex, after which TatA oligomerization supports proton-motive-force-dependent passage across the cytoplasmic membrane. Signal-peptide recognition, receptor assembly, and translocation are modeled as separate substantive roles.

MODULE:bacterial_twin_arginine_translocationDRAFTBiological Processmodules/bacterial_twin_arginine_translocation.yaml
protein transport by the Tat complexGO:0043953
GO:0043953
protein transport by the Tat complex
GO:0043953 defines the biological process shared by TatA-, TatB-, and TatC-family components.
GO:0033281
TAT protein transport complex
GO:0033281 identifies the membrane complex formed by the Tat machinery.
PMID:23530902
The type II secretion system (Xcp) of Pseudomonas putida is active and involved in the secretion of phosphatases.
Primary research in P. putida KT2440 identifies two Tat gene clusters and shows that both complete systems can support transport of UxpB. This supports paralogous module satisfiability without assigning a distinct substrate repertoire to either locus.
Both Tat systems appeared to be capable of transporting the UxpB protein.
file:projects/P_PUTIDA/deep-research/PSEPK__bacterial_twin_arginine_translocation__ppu03070-deep-research-openscientist.md
OpenScientist module, pathway, and taxon review of bacterial twin-arginine translocation in Pseudomonas putida KT2440
The commissioned species-aware review found both PSEPK tatABC loci to satisfy the three reusable roles and identified their primary KEGG assignment as protein export (ppu03060), distinct from downstream Xcp type II secretion in ppu03070.
file:interpro/panther/panther-members.tsv
Curated UniProt-to-PANTHER membership index
The local index verifies the E. coli K-12 and both PSEPK exemplars in PTHR30371:SF0 (TatC), PTHR33162:SF1 (TatB), and PTHR42982:SF1 (TatA).

The reusable boundary starts with recognition of a folded Tat substrate and ends with membrane translocation. Cofactor loading and folding of individual substrates are upstream; signal-peptide cleavage and periplasmic destination functions are downstream. Some bacteria encode multiple complete tatABC loci; their physiological division of labor must not be assumed from sequence alone. In P. putida KT2440, PMID:23530902 shows that both complete systems can transport UxpB, but does not establish isolated-subunit activities or broader locus-specific substrate repertoires. The reusable architecture is modeled once here; copy number, operon-specific regulation, and concrete PSEPK satisfiability belong in the species batch rather than in this module.

4Nodes
3Parts
0Variant Sets
0Variants
3Annotons
2Connections

Derived QC

Recommended-field compliance

55.6% recommended fields populated
  • module.knowledge_gaps[0] · status (0/1)
  • module.knowledge_gaps[0] · provenance (0/1)
  • module.knowledge_gaps[1] · status (0/1)
  • module.knowledge_gaps[1] · provenance (0/1)

Module deep research

✗ none found

No MODULE:bacterial_twin_arginine_translocation deep-research report alongside the module YAML.

Leaf nodes lacking representative members

✓ every leaf node grounds to a representative protein.

Template conformance

✓ every declared conforms_to bundle matches its template motif.

Reaction chaining (advisory)

✓ every PRECEDES step chains, or its break is acknowledged via chaining_status.

  • tatc_substrate_recognition → tata_translocation_assembly [NOT_CHECKED]
    TatC-dependent substrate recognition precedes recruitment of TatA into the active translocation assembly; this is not a metabolite chain.
  • tatb_receptor_organization → tata_translocation_assembly [NOT_CHECKED]
    TatB-dependent receptor organization precedes recruitment of TatA into the active translocation assembly; this is not a metabolite chain.

Gene-review completeness (6/9 grounded genes reviewed)

6 complete review(s) · 1 with deep research · 3 missing review · 5 reviewed but lacking deep research

Gene Review Complete Deep research
Escherichia coli K-12 TatC exemplar P69423 ✗ — —
Escherichia coli K-12 TatB exemplar P69425 ✗ — —
Escherichia coli K-12 TatA exemplar P69428 ✗ — —
tatA-I Q88P12 ✓ ✓ ✗
tatA-II Q88D13 ✓ ✓ ✓
tatB Q88D12 ✓ ✓ ✗
tatB-I Q88P13 ✓ ✓ ✗
tatC-I Q88P14 ✓ ✓ ✗
tatC-II Q88D11 ✓ ✓ ✗

Details

Context
bacteriaNCBITaxon:2
plasma membraneGO:0005886
Bacterial twin-arginine protein translocationBiological Processbacterial_twin_arginine_translocation
protein transport by the Tat complexGO:0043953
Context
bacteriaNCBITaxon:2
plasma membraneGO:0005886

Connections

Part 1: Twin-arginine signal recognition and receptor scaffolding
TatC substrate recognitionBiological Processtatc_substrate_recognition

TatC provides the multispanning membrane scaffold that recognizes twin-arginine signal peptides together with TatB.

Annotons

TatC-family receptor component
tatc_family_component
Participant: Family: TatC family
Family:
TatC familyPANTHER:PTHR30371:SF0
Representative Members: Escherichia coli K-12 TatC exemplarUniProtKB:P69423 PSEPK TatC-I exemplarUniProtKB:Q88P14 PSEPK TatC-II exemplarUniProtKB:Q88D11

Processes

protein transport by the Tat complexGO:0043953

Conserved receptor/scaffold component that initiates productive Tat-complex assembly around a signal-bearing folded substrate.

Part 2: Tat receptor-complex organization
TatB receptor-complex organizationBiological Processtatb_receptor_organization

TatB associates with TatC to organize the substrate receptor and couple substrate recognition to TatA recruitment.

Annotons

TatB-family receptor component
tatb_family_component
Participant: Family: TatB family
Family:
TatB familyPANTHER:PTHR33162:SF1
Representative Members: Escherichia coli K-12 TatB exemplarUniProtKB:P69425 PSEPK TatB-I exemplarUniProtKB:Q88P13 PSEPK TatB exemplar from the second Tat locusUniProtKB:Q88D12

Processes

protein transport by the Tat complexGO:0043953

Receptor-associated component required before recruitment of the TatA translocation assembly.

Part 3: Proton-motive-force-dependent translocation assembly
Active Tat translocon assemblyProtein Complextata_translocation_assembly

TatA is recruited to the substrate-bound TatBC receptor to form the dynamic, proton-motive-force-dependent translocon.

Annotons

Assembled TatABC protein-translocation activity
tatabc_transport_activity
Participant: Protein Complex: bacterial TatABC translocon
Protein Complex:
bacterial TatABC transloconGO:0033281
Active units:
TatA translocation subunit
Participant: Family: TatA family
Family:
TatA familyPANTHER:PTHR42982:SF1
Representative Members: Escherichia coli K-12 TatA exemplarUniProtKB:P69428 PSEPK TatA-I exemplarUniProtKB:Q88P12 PSEPK TatA-II exemplarUniProtKB:Q88D13
Role: Forms the recruited, dynamic membrane assembly required for folded-substrate passage.
TatB receptor subunit
Participant: Family: TatB family
Family:
TatB familyPANTHER:PTHR33162:SF1
Representative Members: Escherichia coli K-12 TatB exemplarUniProtKB:P69425 PSEPK TatB-I exemplarUniProtKB:Q88P13 PSEPK TatB exemplar from the second Tat locusUniProtKB:Q88D12
Role: Couples the substrate-bound receptor to TatA recruitment.
TatC receptor/scaffold subunit
Participant: Family: TatC family
Family:
TatC familyPANTHER:PTHR30371:SF0
Representative Members: Escherichia coli K-12 TatC exemplarUniProtKB:P69423 PSEPK TatC-I exemplarUniProtKB:Q88P14 PSEPK TatC-II exemplarUniProtKB:Q88D11
Role: Recognizes twin-arginine signal peptides with TatB.

Function

proton motive force dependent protein transmembrane transporter activityGO:0009977
Substrates: folded protein bearing a twin-arginine signal peptide
Products: translocated folded protein

Processes

protein transport by the Tat complexGO:0043953

The assembled TatABC machinery carries out membrane translocation after TatBC-dependent substrate recognition and TatA recruitment.