Chemokine-mediated signaling pathway module

Chemokines activate seven-transmembrane chemokine receptors and heterotrimeric G proteins, coordinating PI3K, calcium, and Rho-family pathways that direct leukocyte migration and positioning.

MODULE:chemokine_signalingDRAFTSignaling Pathwaymodules/chemokine_signaling.yaml
chemokine-mediated signaling pathwayGO:0070098
file:modules/chemokine_signaling-deep-research-falcon.md
Chemokine-mediated signaling pathway Falcon module review
Falcon/Edison module review generated 2026-06-28 is linked as supporting evidence for module boundaries, core mechanism, variation, constraints, and failure modes; open questions in that report require manual review before promotion to curated knowledge gaps.
GO:0070098
chemokine-mediated signaling pathway
The module is grounded in the GO biological process term for chemokine-mediated signaling pathway.
PANTHER:PTN000005673
PAINT node PTN000005673 for PIK3CG
Local PAINT IBD node in PTHR10048 with propagated terms C:GO:0005737, C:GO:0016020, F:GO:0052742, P:GO:0046854; PAINT date 20260528.
PANTHER:PTN000005674
PAINT node PTN000005674 for PIK3CG
Local PAINT IBD node in PTHR10048 with propagated terms C:GO:0005886, F:GO:0016303, F:GO:0035005, P:GO:0043491; PAINT date 20240205.
PANTHER:PTN000632671
PAINT node PTN000632671 for RHOA
Local PAINT IBD node in PTHR24072 with propagated terms C:GO:0005886, F:GO:0003924, F:GO:0005525, P:GO:0007015, P:GO:0007165; PAINT date 20260529.
PANTHER:PTN000799655
PAINT node PTN000799655 for PIK3CG
Local PAINT IBD node in PTHR10048 with propagated terms C:GO:0005942; PAINT date 20240516.
PANTHER:PTN000799659
PAINT node PTN000799659 for PIK3CG
Local PAINT IBD node in PTHR10048 with propagated terms C:GO:0005943, C:GO:0005944, F:GO:0046934; PAINT date 20240918.
PANTHER:PTN001178055
PAINT node PTN001178055 for RHOA
Local PAINT IBD node in PTHR24072 with propagated terms P:GO:0016477; PAINT date 20250903.
PANTHER:PTN002784793
PAINT node PTN002784793 for RHOA
Local PAINT IBD node in PTHR24072 with propagated terms P:GO:0007266, P:GO:0043149; PAINT date 20231109.
PANTHER:PTN008393484
PAINT node PTN008393484 for RHOA
Local PAINT IBD node in PTHR24072 with propagated terms C:GO:0005829, P:GO:0032956; PAINT date 20260529.

Curated compact signaling-pathway module generated from verified UniProt exemplar accessions and local PAINT IBD rows. PTN anchors are included only when the exemplar accession appears in an IBD seed row in interpro/panther; roles without local PAINT seeds remain grounded by verified UniProt exemplar accessions. Falcon integration (2026-06-28): Falcon findings are linked as evidence and family-level support. Automatically extracted open-question headings were intentionally not retained as knowledge_gap entries because they were not manually curated. Machine-generated Falcon citations remain curation support and are not manually adjudicated here.

4Nodes
3Parts
0Variant Sets
0Variants
6Annotons
2Connections

Derived QC

Recommended-field compliance

100.0% recommended fields populated

All recommended fields populated.

Module deep research

✓ present

  • chemokine_signaling-deep-research-falcon.md (falcon)

Leaf nodes lacking representative members

✓ every leaf node grounds to a representative protein.

Template conformance

✓ every declared conforms_to bundle matches its template motif.

Gene-review completeness (0/6 grounded genes reviewed)

0 complete review(s) · 0 with deep research · 6 missing review · 0 reviewed but lacking deep research

Gene Review Complete Deep research
GNAI2 P04899 ✗ — —
CXCL8 P10145 ✗ — —
CXCR2 P25025 ✗ — —
PIK3CG P48736 ✗ — —
CCR5 P51681 ✗ — —
RHOA P61586 ✗ — —

Details

Context
metazoaNCBITaxon:33208
plasma membraneGO:0005886 cytosolGO:0005829 nucleusGO:0005634
Chemokine-mediated signaling pathwaySignaling Pathwaychemokine_signaling
chemokine-mediated signaling pathwayGO:0070098
Context
metazoaNCBITaxon:33208
plasma membraneGO:0005886 cytosolGO:0005829 nucleusGO:0005634

Connections

The initiating event promotes heterotrimeric g protein coupling.
Gi protein coupling leads to chemotaxis effector output.
Part 1: chemokine-receptor engagement
Chemokine receptor engagementRegulatory Stepchemokine_receptor_engagement

Chemokine ligands activate GPCR-class chemokine receptors.

Annotons

CXCL8 chemokine ligand
cxcl8_cxcl8_chemokine_ligand
Participant: Gene Product: CXCL8
Gene Product:
CXCL8UniProtKB:P10145

Curated exemplar for the cxcl8 chemokine ligand role in chemokine-mediated signaling pathway.

CXCR2 chemokine receptor
cxcr2_cxcr2_chemokine_receptor
Participant: Gene Product: CXCR2
Gene Product:
CXCR2UniProtKB:P25025

Curated exemplar for the cxcr2 chemokine receptor role in chemokine-mediated signaling pathway.

CCR5 chemokine receptor
ccr5_ccr5_chemokine_receptor
Participant: Gene Product: CCR5
Gene Product:
CCR5UniProtKB:P51681

Curated exemplar for the ccr5 chemokine receptor role in chemokine-mediated signaling pathway.

Part 2: heterotrimeric g protein coupling
Gi protein couplingRegulatory Stepgi_protein_coupling

Activated chemokine receptors couple to Gi-family heterotrimeric G proteins.

Annotons

GNAI2 alpha subunit
gnai2_gnai2_alpha_subunit
Participant: Gene Product: GNAI2
Gene Product:
GNAI2UniProtKB:P04899

Curated exemplar for the gnai2 alpha subunit role in chemokine-mediated signaling pathway.

Part 3: chemotaxis effector output
Chemotaxis effector outputRegulatory Stepchemotaxis_effector_output

PI3K and Rho-family branches coordinate directional migration and actin remodeling.

Annotons

PI3K gamma catalytic branch
pik3cg_pi3k_gamma_catalytic_branch
Participant: Family: PI3K gamma catalytic branch family/ortholog group
Family:
PI3K gamma catalytic branch family/ortholog groupPANTHER:PTHR10048
Representative Members: PIK3CGUniProtKB:P48736

Curated exemplar for the pi3k gamma catalytic branch role in chemokine-mediated signaling pathway.

RhoA cytoskeletal switch
rhoa_rhoa_cytoskeletal_switch
Participant: Family: RhoA cytoskeletal switch family/ortholog group
Family:
RhoA cytoskeletal switch family/ortholog groupPANTHER:PTHR24072
Representative Members: RHOAUniProtKB:P61586

Curated exemplar for the rhoa cytoskeletal switch role in chemokine-mediated signaling pathway.