Aspartate aminotransferases interconvert oxaloacetate and L-aspartate by transferring an amino group from L-glutamate, producing 2-oxoglutarate. Drosophila has a cytosolic isozyme (Got1) and a mitochondrial isozyme (Got2), which also act in the malate-aspartate shuttle. Asparagine synthetase (Asns) then converts aspartate to L-asparagine in an ATP-dependent reaction that uses glutamine as the nitrogen donor.
Boundary. The module covers the three FlyBase members. The malate-aspartate shuttle partners (malate dehydrogenases and mitochondrial carriers) and asparaginase-mediated asparagine breakdown are not parts. Enzyme functions are assigned by orthology to the conserved mammalian enzymes. FlyBase groups realized by this module: ASPASNM (FBgg0002185). Participants were generated from the FlyBase gene-group membership and mapped to UniProtKB by projects/FLYBASE_GENE_GROUPS/generate_modules.py from module_specs/dmel_aspartate_asparagine_metabolism.yaml. Member genes with a gene review in this repository: Asns, Got1, Got2.
All recommended fields populated.
✗ none found
No MODULE:dmel_aspartate_asparagine_metabolism deep-research report alongside the module YAML.
✓ every leaf node grounds to a representative protein.
✓ every declared conforms_to bundle matches its template motif.
✓ every PRECEDES step chains, or its break is acknowledged via chaining_status.
3 gene-function check(s) reflected in core functions · 0 conflict(s) · 0 evidence or coverage gap(s) (3 comparison(s))
Retained noncore functions are reported separately; they do not require promotion to core functions. Support from representative genes does not establish conservation across the whole family. Missing reviews or identifiers leave a claim unverified.
| Annoton | Participant | Function | Assessment |
|---|---|---|---|
| aspartate_transamination_got1 | Got1 UniProtKB:A1ZAA5 Gene |
L-aspartate:2-oxoglutarate transaminase activity GO:0004069 |
Core Supported
The GO molecular function is reflected in core_functions (exact term or a reviewed subclass). |
| aspartate_transamination_got2 | Got2 UniProtKB:Q8IPY3 Gene |
L-aspartate:2-oxoglutarate transaminase activity GO:0004069 |
Core Supported
The GO molecular function is reflected in core_functions (exact term or a reviewed subclass). |
| asparagine_synthesis_asns | Asns UniProtKB:Q7KTW9 Gene |
asparagine synthase (glutamine-hydrolyzing) activity GO:0004066 |
Core Supported
The GO molecular function is reflected in core_functions (exact term or a reviewed subclass). |
2 complete review(s) · 3 with deep research · 0 missing review · 0 reviewed but lacking deep research
| Gene | Review | Complete | Deep research |
|---|---|---|---|
| Asns Q7KTW9 | ✓ | ✓ | ✓ |
| Got1 A1ZAA5 | ✓ | 24/25 | ✓ |
| Got2 Q8IPY3 | ✓ | ✓ | ✓ |
Got1 and Got2 make aspartate from oxaloacetate, and Asns amidates aspartate to asparagine.
Cytosolic (Got1) and mitochondrial (Got2) aspartate aminotransferases; the reaction is reversible.