CCR4-NOT deadenylase complex in Drosophila melanogaster

CCR4-NOT is the major cytoplasmic mRNA deadenylase in eukaryotes and also acts in transcription and translational repression. Its large scaffold subunit Not1 binds two deadenylases, Pop2 (CAF1) and twin (CCR4), which shorten poly(A) tails, the first step of most mRNA decay. Not1 also binds Rcd-1 (CNOT9), Not10 and Not11, and a NOT module of Rga (Not2) and Not3. RNA-binding proteins, microRNA silencing complexes and translational repressors recruit CCR4-NOT to specific mRNAs; in Drosophila it controls maternal mRNA decay and germline stem cell maintenance.

MODULE:dmel_ccr4_not_complexDRAFTCONCRETEProtein Complexmodules/dmel_ccr4_not_complex.yaml
CCR4-NOT complexGO:0030014
FB:FBgg0000469
FlyBase gene group CCR4-NOT: Ccr4-not complex
FlyBase release FB2026_03 groups 8 D. melanogaster genes as ccr4-not complex (CCR4-NOT); this module models that group.
GO:0030014
CCR4-NOT complex
GO term grounding the module as CCR4-NOT complex.

Boundary. The deadenylase subunits are modelled as one complex part; the two enzymes are distinct catalytic subunits that both sit on Not1. Recruiting RNA-binding proteins (for example Nanos, Pumilio, Smaug, Bam) are regulators and not parts. FlyBase groups realized by this module: CCR4-NOT (FBgg0000469). Participants were generated from the FlyBase gene-group membership and mapped to UniProtKB by projects/FLYBASE_GENE_GROUPS/generate_modules.py from module_specs/dmel_ccr4_not_complex.yaml. None of the member genes has a gene review in this repository yet.

4Nodes
3Parts
0Variant Sets
0Variants
3Annotons
0Connections

Derived QC

Recommended-field compliance

100.0% recommended fields populated

All recommended fields populated.

Module deep research

✗ none found

No MODULE:dmel_ccr4_not_complex deep-research report alongside the module YAML.

Leaf nodes lacking representative members

✓ every leaf node grounds to a representative protein.

Template conformance

✓ every declared conforms_to bundle matches its template motif.

Function consistency and core coverage

0 gene-function check(s) reflected in core functions · 0 conflict(s) · 0 evidence or coverage gap(s) (1 comparison(s))

Retained noncore functions are reported separately; they do not require promotion to core functions. Support from representative genes does not establish conservation across the whole family. Missing reviews or identifiers leave a claim unverified.

AnnotonParticipantFunctionAssessment
deadenylase_module_complex PROTEIN_COMPLEX
Gene 		poly(A)-specific ribonuclease activity
GO:0004535 		Participant Unchecked
No direct UniProt participant to compare. Abstract selectors and complex-wide activities cannot be inferred from donor or subunit gene reviews.

Gene-review completeness (0/8 grounded genes reviewed)

0 complete review(s) · 0 with deep research · 8 missing review · 0 reviewed but lacking deep research

Gene Review Complete Deep research
Not3 A0A0B4KEC4 ✗ — —
Not1 A0A0B4LEZ3 ✗ — —
Rcd-1 M9PF85 ✗ — —
twin Q8IMX1 ✗ — —
Not11 Q8MLP8 ✗ — —
Rga Q94547 ✗ — —
Not10 Q9V3G6 ✗ — —
Pop2 X2JCC3 ✗ — —

Details

Context
Drosophila melanogasterNCBITaxon:7227
cytoplasmGO:0005737
CCR4-NOT complexProtein Complexdmel_ccr4_not_complex

Not1-scaffolded Pop2/twin deadenylase complex that shortens mRNA poly(A) tails.

CCR4-NOT complexGO:0030014
Context
Drosophila melanogasterNCBITaxon:7227
cytoplasmGO:0005737
Part 1: poly(A) tail shortening
Deadenylase moduleProtein Complexdeadenylase_module

Annotons

Deadenylase module
deadenylase_module_complex
Participant: Protein Complex: Deadenylase module
Protein Complex:
Deadenylase module
Active units:
Pop2
Participant: Gene Product: Pop2
Gene Product:
Pop2UniProtKB:X2JCC3
twin
Participant: Gene Product: twin
Gene Product:
twinUniProtKB:Q8IMX1

Function

poly(A)-specific ribonuclease activityGO:0004535

Processes

nuclear-transcribed mRNA poly(A) tail shorteningGO:0000289
Part 2: scaffold and recruitment surfaces
Not1 scaffold and associated subunitsProtein Complexnot1_scaffold

Annotons

Not1 scaffold and associated subunits
not1_scaffold_complex
Participant: Protein Complex: Not1 scaffold and associated subunits
Protein Complex:
Not1 scaffold and associated subunits
Active units:
Not1
Participant: Gene Product: Not1
Gene Product:
Not1UniProtKB:A0A0B4LEZ3
Rcd-1
Participant: Gene Product: Rcd-1
Gene Product:
Rcd-1UniProtKB:M9PF85
Not10
Participant: Gene Product: Not10
Gene Product:
Not10UniProtKB:Q9V3G6
Not11
Participant: Gene Product: Not11
Gene Product:
Not11UniProtKB:Q8MLP8
Part 3: Not2-Not3 module
NOT moduleProtein Complexnot_module

Annotons

NOT module
not_module_complex
Participant: Protein Complex: NOT module
Protein Complex:
NOT module
Active units:
Rga
Participant: Gene Product: Rga
Gene Product:
RgaUniProtKB:Q94547
Not3
Participant: Gene Product: Not3
Gene Product:
Not3UniProtKB:A0A0B4KEC4