Chromatin assembly factor 1 in Drosophila melanogaster

Chromatin assembly factor 1 (CAF-1) is a three-subunit histone chaperone that deposits newly synthesised histone H3-H4 onto DNA behind the replication fork, coupling nucleosome assembly to DNA replication through its interaction with PCNA. It consists of Caf1-180 (p180), Caf1-105 (p105) and the WD-repeat histone-binding protein Caf1-55 (p55). Caf1-55 is also a subunit of several other chromatin complexes.

MODULE:dmel_chromatin_assembly_factor_1DRAFTCONCRETEProtein Complexmodules/dmel_chromatin_assembly_factor_1.yaml
CAF-1 complexGO:0033186
FB:FBgg0000295
FlyBase gene group CAF-1: Chromatin assembly factor 1
FlyBase release FB2026_03 groups 3 D. melanogaster genes as chromatin assembly factor 1 (CAF-1); this module models that group.
GO:0033186
CAF-1 complex
GO term grounding the module as CAF-1 complex.

A three-subunit complex decomposed one part per subunit. Caf1-55 is shared with NURF, NuRD, PRC2 and MuvB complexes, which are modelled in their own modules. The histone H3-H4 chaperone activity (GO:0000510), which Caf1-180 carries in GOA by IBA, is recorded on a complex-level annoton with all three subunits as active units. FlyBase groups realized by this module: CAF-1 (FBgg0000295). Participants were generated from the FlyBase gene-group membership and mapped to UniProtKB by projects/FLYBASE_GENE_GROUPS/generate_modules.py from module_specs/dmel_chromatin_assembly_factor_1.yaml. Member genes with a gene review in this repository: Caf1-105, Caf1-180, Caf1-55.

4Nodes
3Parts
0Variant Sets
0Variants
4Annotons
0Connections

Derived QC

Recommended-field compliance

100.0% recommended fields populated

All recommended fields populated.

Module deep research

✗ none found

No MODULE:dmel_chromatin_assembly_factor_1 deep-research report alongside the module YAML.

Leaf nodes lacking representative members

✓ every leaf node grounds to a representative protein.

Template conformance

✓ every declared conforms_to bundle matches its template motif.

Function consistency and core coverage

0 gene-function check(s) reflected in core functions · 0 conflict(s) · 0 evidence or coverage gap(s) (1 comparison(s))

Retained noncore functions are reported separately; they do not require promotion to core functions. Support from representative genes does not establish conservation across the whole family. Missing reviews or identifiers leave a claim unverified.

AnnotonParticipantFunctionAssessment
dmel_chromatin_assembly_factor_1_complex_activity PROTEIN_COMPLEX
Gene 		H3-H4 histone complex chaperone activity
GO:0000510 		Participant Unchecked
No direct UniProt participant to compare. Abstract selectors and complex-wide activities cannot be inferred from donor or subunit gene reviews.

Gene-review completeness (3/3 grounded genes reviewed)

2 complete review(s) · 3 with deep research · 0 missing review · 0 reviewed but lacking deep research

Gene Review Complete Deep research
Caf1-105 A1Z898 ✓ ✓ ✓
Caf1-180 Q9W3D1 ✓ ✓ ✓
Caf1-55 Q24572 ✓ 90/91 ✓

Details

Context
Drosophila melanogasterNCBITaxon:7227
chromatinGO:0000785
CAF-1Protein Complexdmel_chromatin_assembly_factor_1

Three-subunit histone H3-H4 chaperone that assembles nucleosomes during DNA replication.

CAF-1 complexGO:0033186
Context
Drosophila melanogasterNCBITaxon:7227
chromatinGO:0000785

Annotons

CAF-1 activity
dmel_chromatin_assembly_factor_1_complex_activity
Participant: Protein Complex: CAF-1
Protein Complex:
CAF-1GO:0033186
Active units:
Caf1-105
Participant: Gene Product: Caf1-105
Gene Product:
Caf1-105UniProtKB:A1Z898
Role: middle subunit
Caf1-180
Participant: Gene Product: Caf1-180
Gene Product:
Caf1-180UniProtKB:Q9W3D1
Role: large subunit
Caf1-55
Participant: Gene Product: Caf1-55
Gene Product:
Caf1-55UniProtKB:Q24572
Role: WD-repeat histone-binding subunit

Function

H3-H4 histone complex chaperone activityGO:0000510

Delivers newly synthesized histone H3-H4 to replicating DNA for replication-coupled nucleosome assembly.

Part 1: middle subunit
Caf1-105Moduledmel_chromatin_assembly_factor_1_caf1_105

Annotons

Caf1-105 subunit
dmel_chromatin_assembly_factor_1_caf1_105_caf1_105
Participant: Gene Product: Caf1-105
Gene Product:
Caf1-105UniProtKB:A1Z898

middle subunit

Part 2: large subunit
Caf1-180Moduledmel_chromatin_assembly_factor_1_caf1_180

Annotons

Caf1-180 subunit
dmel_chromatin_assembly_factor_1_caf1_180_caf1_180
Participant: Gene Product: Caf1-180
Gene Product:
Caf1-180UniProtKB:Q9W3D1

large subunit

Part 3: WD-repeat histone-binding subunit
Caf1-55Moduledmel_chromatin_assembly_factor_1_caf1_55

Annotons

Caf1-55 subunit
dmel_chromatin_assembly_factor_1_caf1_55_caf1_55
Participant: Gene Product: Caf1-55
Gene Product:
Caf1-55UniProtKB:Q24572

WD-repeat histone-binding subunit