Most eukaryotic mRNAs acquire their 3' end by endonucleolytic cleavage of the pre-mRNA followed by addition of a poly(A) tail. The cleavage and polyadenylation specificity factor (CPSF) recognizes the AAUAAA polyadenylation signal through Cpsf160, Wdr33 and Clp (CPSF30), and Fip1 recruits poly(A) polymerase. The cleavage stimulation factor (CstF; CstF50, CstF64 and Su(f), the CstF77 homolog) binds GU-rich sequences downstream of the cleavage site. The core cleavage complex of Cpsf73, Cpsf100 and the scaffold Symplekin (Sym) carries out cleavage, with Cpsf73 as the endonuclease. Cpsf73, Cpsf100 and Sym are shared with the histone pre-mRNA processing machinery.
Boundary. FlyBase groups the polyadenylation factors (PF: CPSF signal recognition subunits plus CstF) separately from the core cleavage complex (CCC); this module splits PF into its CPSF and CstF subcomplexes. Poly(A) polymerase, the CFIm and CFIIm factors and poly(A)-binding proteins have no FlyBase group in this set and are not added. The histone pre-mRNA processing machinery that reuses the core cleavage complex is in dmel_histone_mrna_3prime_processing. FlyBase groups realized by this module: PF (FBgg0000618), CCC (FBgg0000621). Participants were generated from the FlyBase gene-group membership and mapped to UniProtKB by projects/FLYBASE_GENE_GROUPS/generate_modules.py from module_specs/dmel_cleavage_polyadenylation.yaml. None of the member genes has a gene review in this repository yet.
All recommended fields populated.
✗ none found
No MODULE:dmel_cleavage_polyadenylation deep-research report alongside the module YAML.
✓ every leaf node grounds to a representative protein.
✓ every declared conforms_to bundle matches its template motif.
0 gene-function check(s) reflected in core functions · 0 conflict(s) · 1 evidence or coverage gap(s) (1 comparison(s))
Retained noncore functions are reported separately; they do not require promotion to core functions. Support from representative genes does not establish conservation across the whole family. Missing reviews or identifiers leave a claim unverified.
| Annoton | Participant | Function | Assessment |
|---|---|---|---|
| core_cleavage_complex_complex/cpsf73 | Cpsf73 UniProtKB:Q9VE51 Complex Unit |
RNA endonuclease activity GO:0004521 |
Review Missing
No ai-review exists for this protein; its function and core-function coverage cannot yet be checked. |
0 complete review(s) · 0 with deep research · 10 missing review · 0 reviewed but lacking deep research
| Gene | Review | Complete | Deep research |
|---|---|---|---|
| su(f) P25991 | ✗ | — | — |
| Sym Q8MSU4 | ✗ | — | — |
| Cpsf100 Q9V3D6 | ✗ | — | — |
| Cpsf160 Q9V726 | ✗ | — | — |
| CstF50 Q9V9V0 | ✗ | — | — |
| Cpsf73 Q9VE51 | ✗ | — | — |
| CstF64 Q9VE52 | ✗ | — | — |
| Fip1 Q9VN31 | ✗ | — | — |
| Wdr33 Q9VNG2 | ✗ | — | — |
| Clp Q9VPT8 | ✗ | — | — |
CPSF, CstF and the Cpsf73-Cpsf100-Symplekin core cleavage complex, which cleave pre-mRNAs at the polyadenylation site.