The calcium release-activated calcium (CRAC) channel mediates store-operated calcium entry. Stim is a single-pass endoplasmic reticulum membrane protein whose luminal EF-hand senses ER calcium; when ER stores are depleted, Stim oligomerises, moves to ER-plasma membrane junctions and binds and opens Orai, the pore-forming plasma membrane channel subunit. Stim and Orai were identified in Drosophila S2 cell RNAi screens as essential for store-operated calcium entry.
GO has no specific term for the CRAC channel complex, so the parent term calcium channel complex is used. Stim and Orai sit in different membranes and interact at ER-plasma membrane junctions after store depletion. FlyBase groups realized by this module: CRAC (FBgg0000592). Participants were generated from the FlyBase gene-group membership and mapped to UniProtKB by projects/FLYBASE_GENE_GROUPS/generate_modules.py from module_specs/dmel_crac_channel_complex.yaml. Member genes with a gene review in this repository: Orai, Stim.
All recommended fields populated.
✗ none found
No MODULE:dmel_crac_channel_complex deep-research report alongside the module YAML.
✓ every leaf node grounds to a representative protein.
✓ every declared conforms_to bundle matches its template motif.
2 gene-function check(s) reflected in core functions · 0 conflict(s) · 0 evidence or coverage gap(s) (2 comparison(s))
Retained noncore functions are reported separately; they do not require promotion to core functions. Support from representative genes does not establish conservation across the whole family. Missing reviews or identifiers leave a claim unverified.
| Annoton | Participant | Function | Assessment |
|---|---|---|---|
| dmel_crac_channel_complex_orai_orai | Orai UniProtKB:Q9U6B8 Gene |
store-operated calcium channel activity GO:0015279 |
Core Supported
The GO molecular function is reflected in core_functions (exact term or a reviewed subclass). |
| dmel_crac_channel_complex_stim_stim | Stim UniProtKB:P83094 Gene |
calcium channel regulator activity GO:0005246 |
Core Supported
The GO molecular function is reflected in core_functions (exact term or a reviewed subclass). |
2 complete review(s) · 2 with deep research · 0 missing review · 0 reviewed but lacking deep research
| Gene | Review | Complete | Deep research |
|---|---|---|---|
| Orai Q9U6B8 | ✓ | ✓ | ✓ |
| Stim P83094 | ✓ | ✓ | ✓ |
Stim ER calcium sensor and Orai plasma membrane pore that together mediate store-operated calcium entry.
pore-forming plasma membrane subunit
ER calcium sensor and channel activator