NADPH supplies reducing power for fatty acid synthesis and for antioxidant systems such as glutathione and thioredoxin reduction. In Drosophila, as in other animals, it is regenerated outside mitochondria by three sets of NADP-dependent dehydrogenases. The oxidative branch of the pentose phosphate pathway produces two NADPH per glucose 6-phosphate through glucose-6-phosphate dehydrogenase (G6pd, Zw) and 6-phosphogluconate dehydrogenase (Pgd). NADP-dependent isocitrate dehydrogenase (Idh) oxidizes isocitrate to 2-oxoglutarate, and NADP-dependent malic enzyme (Men) decarboxylates malate to pyruvate, each reducing NADP+. Studies of fly mutants and allozyme variants link these enzymes to lipid accumulation.
Boundary. The module covers the five FlyBase NADPH regeneration enzymes. The three parts are parallel sources of NADPH and are not linked by connections. The 6-phosphogluconolactonase step between G6pd and Pgd is not a FlyBase member and is not modelled. Mitochondrial NADPH sources (for example nicotinamide nucleotide transhydrogenase) are outside the module. FlyBase groups realized by this module: NADPHRE (FBgg0002243). Participants were generated from the FlyBase gene-group membership and mapped to UniProtKB by projects/FLYBASE_GENE_GROUPS/generate_modules.py from module_specs/dmel_cytosolic_nadph_regeneration.yaml. Member genes with a gene review in this repository: G6pd, Idh, Men, Men-b, Pgd.
All recommended fields populated.
✗ none found
No MODULE:dmel_cytosolic_nadph_regeneration deep-research report alongside the module YAML.
✓ every leaf node grounds to a representative protein.
✓ every declared conforms_to bundle matches its template motif.
4 gene-function check(s) reflected in core functions · 0 conflict(s) · 0 evidence or coverage gap(s) (4 comparison(s))
Retained noncore functions are reported separately; they do not require promotion to core functions. Support from representative genes does not establish conservation across the whole family. Missing reviews or identifiers leave a claim unverified.
| Annoton | Participant | Function | Assessment |
|---|---|---|---|
| g6p_dehydrogenation_g6pd | G6pd UniProtKB:P12646 Gene |
glucose-6-phosphate dehydrogenase activity GO:0004345 |
Core Supported
The GO molecular function is reflected in core_functions (exact term or a reviewed subclass). |
| phosphogluconate_decarboxylation_pgd | Pgd UniProtKB:P41572 Gene |
phosphogluconate dehydrogenase (decarboxylating) activity GO:0004616 |
Core Supported
The GO molecular function is reflected in core_functions (exact term or a reviewed subclass). |
| isocitrate_oxidation_idh | Idh UniProtKB:B7Z0E0 Gene |
isocitrate dehydrogenase (NADP+) activity GO:0004450 |
Core Supported
The GO molecular function is reflected in core_functions (exact term or a reviewed subclass). |
| malate_decarboxylation_men | Men UniProtKB:Q9VG31 Gene |
malate dehydrogenase (decarboxylating) (NADP+) activity GO:0004473 |
Core Supported
The GO molecular function is reflected in core_functions (exact term or a reviewed subclass). |
5 complete review(s) · 5 with deep research · 0 missing review · 0 reviewed but lacking deep research
| Gene | Review | Complete | Deep research |
|---|---|---|---|
| G6pd P12646 | ✓ | ✓ | ✓ |
| Idh B7Z0E0 | ✓ | ✓ | ✓ |
| Men Q9VG31 | ✓ | ✓ | ✓ |
| Men-b E1JIZ4 | ✓ | ✓ | ✓ |
| Pgd P41572 | ✓ | ✓ | ✓ |
NADPH regeneration by the oxidative pentose phosphate pathway (G6pd, Pgd), NADP-isocitrate dehydrogenase (Idh) and malic enzymes (Men, Men-b).
Men is the main NADP-malic enzyme; Men-b is a paralog whose activity has not been characterized.