Diphthamide is a modified histidine residue on translation elongation factor 2 (eEF2) that is required for accurate translation and is the target of diphtheria toxin. Its first step, transfer of a 3-amino-3-carboxypropyl group from S-adenosylmethionine to the histidine of eEF2, is catalysed by a heterodimer of the iron-sulfur radical SAM-like enzymes Dph1 and Dph2. Later steps are performed by Dph3, Dph4, Dph5, Dph6 and Dph7, which are not part of this complex.
Modelled one part per subunit. The activity belongs to the heterodimer and is given to both subunits; assignments are by orthology to yeast and human Dph1-Dph2. FlyBase groups realized by this module: DPH1-DPH2 (FBgg0001996). Participants were generated from the FlyBase gene-group membership and mapped to UniProtKB by projects/FLYBASE_GENE_GROUPS/generate_modules.py from module_specs/dmel_diphthamide_dph1_dph2_complex.yaml. Member genes with a gene review in this repository: Dph1, Dph2.
All recommended fields populated.
✗ none found
No MODULE:dmel_diphthamide_dph1_dph2_complex deep-research report alongside the module YAML.
✓ every leaf node grounds to a representative protein.
✓ every declared conforms_to bundle matches its template motif.
0 gene-function check(s) reflected in core functions · 0 conflict(s) · 2 evidence or coverage gap(s) (2 comparison(s))
Retained noncore functions are reported separately; they do not require promotion to core functions. Support from representative genes does not establish conservation across the whole family. Missing reviews or identifiers leave a claim unverified.
| Annoton | Participant | Function | Assessment |
|---|---|---|---|
| dph1_subunit_dph1 | Dph1 UniProtKB:Q9VTM2 Gene |
2-(3-amino-3-carboxypropyl)histidine synthase activity GO:0090560 |
Annotation Supported
A retained annotation or curated replacement supports this function, but core_functions does not yet reflect it. |
| dph2_subunit_dph2 | Dph2 UniProtKB:Q9VFE9 Gene |
2-(3-amino-3-carboxypropyl)histidine synthase activity GO:0090560 |
Annotation Supported
A retained annotation or curated replacement supports this function, but core_functions does not yet reflect it. |
2 complete review(s) · 1 with deep research · 0 missing review · 1 reviewed but lacking deep research
| Gene | Review | Complete | Deep research |
|---|---|---|---|
| Dph1 Q9VTM2 | ✓ | ✓ | ✓ |
| Dph2 Q9VFE9 | ✓ | ✓ | ✗ |
Dph1-Dph2 heterodimer that catalyses the first step of eEF2 diphthamide synthesis.