Cup is an ovary-enriched eIF4E-binding protein that represses translation of specific maternal mRNAs during oogenesis. Cup binds the cap-binding factor eIF4E1 through a motif resembling that of eIF4G, so that eIF4E1 bound to the mRNA cap cannot recruit eIF4G and the ribosome. Cup is recruited to target mRNAs such as oskar and nanos by sequence-specific RNA-binding repressors (Bruno and Smaug), so that these mRNAs stay silent until they are localized in the oocyte. Cup is also required for eIF4E localization to the oocyte.
Two-subunit complex modelled one part per subunit. Bruno (aret) and Smaug (smg), which recruit the complex to target mRNAs, are not FlyBase members and are not modelled as parts. eIF4E1 also acts in general cap-dependent translation initiation with eIF4G, outside this module. FlyBase groups realized by this module: ECUP (FBgg0001981). Participants were generated from the FlyBase gene-group membership and mapped to UniProtKB by projects/FLYBASE_GENE_GROUPS/generate_modules.py from module_specs/dmel_eif4e_cup_complex.yaml. Member genes with a gene review in this repository: cup, eIF4E1.
All recommended fields populated.
✗ none found
No MODULE:dmel_eif4e_cup_complex deep-research report alongside the module YAML.
✓ every leaf node grounds to a representative protein.
✓ every declared conforms_to bundle matches its template motif.
1 gene-function check(s) reflected in core functions · 0 conflict(s) · 1 evidence or coverage gap(s) (2 comparison(s))
Retained noncore functions are reported separately; they do not require promotion to core functions. Support from representative genes does not establish conservation across the whole family. Missing reviews or identifiers leave a claim unverified.
| Annoton | Participant | Function | Assessment |
|---|---|---|---|
| eif4e1_subunit_eif4e1 | eIF4E1 UniProtKB:P48598 Gene |
RNA 7-methylguanosine cap binding GO:0000340 |
Annotation Supported
A retained annotation or curated replacement supports this function, but core_functions does not yet reflect it. |
| cup_subunit_cup | cup UniProtKB:Q9VMA3 Gene |
eukaryotic initiation factor 4E binding GO:0008190 |
Core Supported
The GO molecular function is reflected in core_functions (exact term or a reviewed subclass). |
0 complete review(s) · 2 with deep research · 0 missing review · 0 reviewed but lacking deep research
| Gene | Review | Complete | Deep research |
|---|---|---|---|
| cup Q9VMA3 | ✓ | 55/57 | ✓ |
| eIF4E1 P48598 | ✓ | 60/62 | ✓ |
Cup-eIF4E1 complex that blocks cap-dependent translation initiation on localized maternal mRNAs.