ESCRT-0
escrt_0_complex
Participant: Protein Complex: ESCRT-0
Protein Complex:
ESCRT machinery in Drosophila melanogaster
The endosomal sorting complexes required for transport (ESCRTs) sort ubiquitylated membrane proteins into intralumenal vesicles of multivesicular bodies and drive membrane scission that buds away from the cytoplasm. ESCRT-0 (Hrs, Stam) binds ubiquitylated cargo on endosomes; ESCRT-I and ESCRT-II bind cargo and membrane and nucleate ESCRT-III, whose subunits polymerize into spirals that constrict the bud neck. The AAA ATPase Vps4, with its cofactor Vta1, disassembles ESCRT-III and recycles it. The same ESCRT-III and Vps4 machinery acts in cytokinetic abscission, nuclear envelope sealing and membrane repair. In Drosophila, loss of ESCRT function causes accumulation of signalling receptors such as Notch on endosomes and neoplastic overgrowth in epithelia.
endosome transport via multivesicular body sorting pathwayGO:0032509
FB:FBgg0000215
FlyBase gene group ESCRT: Endosomal sorting complexes required for transport
FlyBase release FB2026_03 groups 20 D. melanogaster genes as endosomal sorting complexes required for transport (ESCRT); this module models that group.
GO:0032509
endosome transport via multivesicular body sorting pathway
GO term grounding the module as endosome transport via multivesicular body sorting pathway.
Decomposition. The five ESCRT subcomplexes are modelled as ordered parts, following the sequential recruitment established for the MVB pathway. The two ESCRT-I variants that differ in their Vps37 paralog are alternatives. Accessory factors such as ALiX are outside the module. FlyBase groups realized by this module: ESCRT (FBgg0000215). Subgroups folded into it: VSP4C, ESCRT-0, ESCRT-III, ESCRT-I, ESCRT-II, ESCRT-IB, ESCRT-IA. Participants were generated from the FlyBase gene-group membership and mapped to UniProtKB by projects/FLYBASE_GENE_GROUPS/generate_modules.py from module_specs/dmel_escrt_machinery.yaml. None of the member genes has a gene review in this repository yet.
8Nodes
5Parts
1Variant Sets
2Variants
6Annotons
4Connections
Derived QC
Recommended-field compliance
100.0%
recommended fields populated
All recommended fields populated.
Module deep research
✗ none found
No MODULE:dmel_escrt_machinery deep-research report alongside the module YAML.
Leaf nodes lacking representative members
✓ every leaf node grounds to a representative protein.
Template conformance
✓ every declared conforms_to bundle matches its template motif.
Reaction chaining (advisory)
✓ every PRECEDES step chains, or its break is acknowledged via chaining_status.
- escrt_0 → escrt_i [NOT_CHECKED]
- escrt_i → escrt_ii [NOT_CHECKED]
- escrt_ii → escrt_iii [NOT_CHECKED]
- escrt_iii → vps4_vta1 [NOT_CHECKED]
Function consistency and core coverage
0 gene-function check(s) reflected in core functions · 0 conflict(s) · 1 evidence or coverage gap(s) (1 comparison(s))
Retained noncore functions are reported separately; they do not require promotion to core functions. Support from representative genes does not establish conservation across the whole family. Missing reviews or identifiers leave a claim unverified.
| Annoton | Participant | Function | Assessment |
|---|---|---|---|
| vps4_vta1_complex/vps4 | Vps4 UniProtKB:Q9Y162 Complex Unit |
ATP hydrolysis activity GO:0016887 |
Review Missing
No ai-review exists for this protein; its function and core-function coverage cannot yet be checked. |
Gene-review completeness (0/20 grounded genes reviewed)
0 complete review(s) · 0 with deep research · 20 missing review · 0 reviewed but lacking deep research
| Gene | Review | Complete | Deep research |
|---|---|---|---|
| Vsp37A O77259 | ✗ | — | — |
| Vps25 Q7JXV9 | ✗ | — | — |
| CG5498 Q7KTZ0 | ✗ | — | — |
| Chmp1 Q7KUS3 | ✗ | — | — |
| shrb Q8T0Q4 | ✗ | — | — |
| Hrs Q960X8 | ✗ | — | — |
| Vps28 Q9V359 | ✗ | — | — |
| Vps2 Q9VBI3 | ✗ | — | — |
| lsn Q9VD72 | ✗ | — | — |
| Vps24 Q9VN02 | ✗ | — | — |
| Vps37B Q9VN88 | ✗ | — | — |
| CHMP2B Q9VRJ5 | ✗ | — | — |
| Vps36 Q9VU87 | ✗ | — | — |
| TSG101 Q9VVA7 | ✗ | — | — |
| Vps60 Q9VVI9 | ✗ | — | — |
| Mvb12 Q9VWY7 | ✗ | — | — |
| Vta1 Q9W0B3 | ✗ | — | — |
| Vps20 Q9W236 | ✗ | — | — |
| Stam Q9XTL2 | ✗ | — | — |
| Vps4 Q9Y162 | ✗ | — | — |
Details
Context
Drosophila melanogasterNCBITaxon:7227
endosome membraneGO:0010008
ESCRT machineryProtein Complexdmel_escrt_machinery
ESCRT-0, -I, -II and -III with Vps4-Vta1 that sort ubiquitylated cargo into multivesicular bodies and drive outward membrane scission.
endosome transport via multivesicular body sorting pathwayGO:0032509
Context
Drosophila melanogasterNCBITaxon:7227
endosome membraneGO:0010008
Connections
Part 1: ubiquitylated cargo clustering
ESCRT-0Protein Complexescrt_0
Annotons
Part 2: cargo binding and ESCRT-II recruitment
ESCRT-IProtein Complexescrt_i
Variant set: ESCRT-I Vps37A or Vps37B variant (One Or More)
ESCRT-I Vps37A variantProtein Complexescrt_ia
Annotons
ESCRT-I Vps37A variant
escrt_ia_complex
Participant: Protein Complex: ESCRT-I Vps37A variant
Protein Complex:
ESCRT-I Vps37B variantProtein Complexescrt_ib
Annotons
ESCRT-I Vps37B variant
escrt_ib_complex
Participant: Protein Complex: ESCRT-I Vps37B variant
Protein Complex:
Part 3: ESCRT-III nucleation
ESCRT-IIProtein Complexescrt_ii
Annotons
ESCRT-II
escrt_ii_complex
Participant: Protein Complex: ESCRT-II
Protein Complex:
Part 4: membrane-constricting polymer
ESCRT-IIIProtein Complexescrt_iii
Annotons
ESCRT-III
escrt_iii_complex
Participant: Protein Complex: ESCRT-III
Protein Complex:
Processes
membrane fissionGO:0090148
Part 5: ESCRT-III disassembly ATPase
Vps4-Vta1Protein Complexvps4_vta1
Annotons
Vps4-Vta1
vps4_vta1_complex
Participant: Protein Complex: Vps4-Vta1
Protein Complex:
Vps4-Vta1
Active units:
Vps4
Participant: Gene Product: Vps4
Gene Product:
Vps4UniProtKB:Q9Y162
Function:
ATP hydrolysis activityGO:0016887