Mitochondrial glutamyl-tRNA(Gln) amidotransferase (GatCAB) in Drosophila melanogaster

In mitochondria of animals there is no glutaminyl-tRNA synthetase. Instead, mitochondrial tRNA-Gln is first mischarged with glutamate by the non-discriminating mitochondrial glutamyl-tRNA synthetase, and the heterotrimeric GatCAB amidotransferase converts the bound glutamate to glutamine. GatA is a glutaminase that releases ammonia from glutamine, GatB phosphorylates the glutamate and transfers the ammonia to form Gln-tRNA(Gln), and GatC is a small subunit that stabilizes the complex.

MODULE:dmel_glutamyl_trna_amidotransferaseDRAFTCONCRETEProtein Complexmodules/dmel_glutamyl_trna_amidotransferase.yaml
glutamyl-tRNA(Gln) amidotransferase complexGO:0030956
FB:FBgg0002000
FlyBase gene group GATCAB: Glutamyl-trna(gln) amidotransferase complex
FlyBase release FB2026_03 groups 3 D. melanogaster genes as glutamyl-trna(gln) amidotransferase complex (GATCAB); this module models that group.
GO:0030956
glutamyl-tRNA(Gln) amidotransferase complex
GO term grounding the module as glutamyl-tRNA(Gln) amidotransferase complex.

Modelled one part per subunit. The glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity (GO:0050567) belongs to the assembled complex and is recorded on a complex-level annoton whose active units are GatA (glutaminase site) and GatB (kinase and transamidation site); GatC has no catalytic site and is not an active unit. GatA and GatB both carry GO:0050567 in GOA (IBA, ISS). The mitochondrial glutamyl-tRNA synthetase that provides the substrate is outside this module. FlyBase groups realized by this module: GATCAB (FBgg0002000). Participants were generated from the FlyBase gene-group membership and mapped to UniProtKB by projects/FLYBASE_GENE_GROUPS/generate_modules.py from module_specs/dmel_glutamyl_trna_amidotransferase.yaml. Member genes with a gene review in this repository: GatA, GatB, GatC.

4Nodes
3Parts
0Variant Sets
0Variants
4Annotons
0Connections

Derived QC

Recommended-field compliance

100.0% recommended fields populated

All recommended fields populated.

Module deep research

✗ none found

No MODULE:dmel_glutamyl_trna_amidotransferase deep-research report alongside the module YAML.

Leaf nodes lacking representative members

✓ every leaf node grounds to a representative protein.

Template conformance

✓ every declared conforms_to bundle matches its template motif.

Function consistency and core coverage

0 gene-function check(s) reflected in core functions · 0 conflict(s) · 0 evidence or coverage gap(s) (1 comparison(s))

Retained noncore functions are reported separately; they do not require promotion to core functions. Support from representative genes does not establish conservation across the whole family. Missing reviews or identifiers leave a claim unverified.

AnnotonParticipantFunctionAssessment
dmel_glutamyl_trna_amidotransferase_complex_activity PROTEIN_COMPLEX
Gene 		glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity
GO:0050567 		Participant Unchecked
No direct UniProt participant to compare. Abstract selectors and complex-wide activities cannot be inferred from donor or subunit gene reviews.

Gene-review completeness (3/3 grounded genes reviewed)

3 complete review(s) · 3 with deep research · 0 missing review · 0 reviewed but lacking deep research

Gene Review Complete Deep research
GatA Q9VE09 ✓ ✓ ✓
GatB Q9VCD0 ✓ ✓ ✓
GatC Q86BL4 ✓ ✓ ✓

Details

Context
Drosophila melanogasterNCBITaxon:7227
mitochondrionGO:0005739
GatCAB amidotransferaseProtein Complexdmel_glutamyl_trna_amidotransferase

Mitochondrial GatA-GatB-GatC complex that forms Gln-tRNA(Gln) by transamidation.

glutamyl-tRNA(Gln) amidotransferase complexGO:0030956
Context
Drosophila melanogasterNCBITaxon:7227
mitochondrionGO:0005739

Annotons

GatCAB amidotransferase activity
dmel_glutamyl_trna_amidotransferase_complex_activity
Participant: Protein Complex: GatCAB amidotransferase
Protein Complex:
GatCAB amidotransferaseGO:0030956
Active units:
GatA
Participant: Gene Product: GatA
Gene Product:
GatAUniProtKB:Q9VE09
GatB
Participant: Gene Product: GatB
Gene Product:
GatBUniProtKB:Q9VCD0

Function

glutaminyl-tRNA synthase (glutamine-hydrolyzing) activityGO:0050567

Processes

tRNA aminoacylation for mitochondrial protein translationGO:0070127

Locations

mitochondrionGO:0005739

Converts Glu-tRNA(Gln), made by the non-discriminating mitochondrial glutamyl-tRNA synthetase, to Gln-tRNA(Gln) using glutamine as the amide donor and ATP.

Part 1: glutaminase subunit
GatA subunitProtein Complexgata_subunit

Annotons

GatA GatA subunit
gata_subunit_gata
Participant: Gene Product: GatA
Gene Product:
GatAUniProtKB:Q9VE09

Processes

tRNA aminoacylation for mitochondrial protein translationGO:0070127
Part 2: kinase and transamidation subunit
GatB subunitProtein Complexgatb_subunit

Annotons

GatB GatB subunit
gatb_subunit_gatb
Participant: Gene Product: GatB
Gene Product:
GatBUniProtKB:Q9VCD0

Processes

tRNA aminoacylation for mitochondrial protein translationGO:0070127
Part 3: stabilizing subunit
GatC subunitProtein Complexgatc_subunit

Annotons

GatC GatC subunit
gatc_subunit_gatc
Participant: Gene Product: GatC
Gene Product:
GatCUniProtKB:Q86BL4

Processes

tRNA aminoacylation for mitochondrial protein translationGO:0070127