GTPBP3-MTO1 mitochondrial tRNA modification complex in Drosophila melanogaster

GTPBP3 and MTO1, the mitochondrial orthologs of bacterial MnmE and MnmG, act together to install a taurinomethyl group at the wobble uridine (U34) of several mitochondrial tRNAs, forming taurinomethyluridine (taum5U). This modification supports accurate decoding during mitochondrial translation. GTPBP3 is a GTPase that binds the methylene donor, and MTO1 is an FAD-dependent enzyme. In Drosophila the orthologs are CG18528 (GTPBP3) and CG4610 (MTO1).

MODULE:dmel_gtpbp3_mto1_complexDRAFTCONCRETEProtein Complexmodules/dmel_gtpbp3_mto1_complex.yaml
GTPBP3-MTO1 complexGO:7770010
FB:FBgg0002142
FlyBase gene group GBP3MC: Gtpbp3-mto1 complex
FlyBase release FB2026_03 groups 2 D. melanogaster genes as gtpbp3-mto1 complex (GBP3MC); this module models that group.
GO:7770010
GTPBP3-MTO1 complex
GO term grounding the module as GTPBP3-MTO1 complex.

Modelled one part per subunit. Subunit functions are inferred by orthology to human GTPBP3 and MTO1 and bacterial MnmE and MnmG; neither Drosophila protein has been characterized biochemically. GTPase activity is assigned to CG18528 on the basis of its conserved TrmE-type G domain. FlyBase groups realized by this module: GBP3MC (FBgg0002142). Participants were generated from the FlyBase gene-group membership and mapped to UniProtKB by projects/FLYBASE_GENE_GROUPS/generate_modules.py from module_specs/dmel_gtpbp3_mto1_complex.yaml. Member genes with a gene review in this repository: CG18528, CG4610.

3Nodes
2Parts
0Variant Sets
0Variants
2Annotons
0Connections

Derived QC

Recommended-field compliance

100.0% recommended fields populated

All recommended fields populated.

Module deep research

✗ none found

No MODULE:dmel_gtpbp3_mto1_complex deep-research report alongside the module YAML.

Leaf nodes lacking representative members

✓ every leaf node grounds to a representative protein.

Template conformance

✓ every declared conforms_to bundle matches its template motif.

Function consistency and core coverage

1 gene-function check(s) reflected in core functions · 0 conflict(s) · 0 evidence or coverage gap(s) (1 comparison(s))

Retained noncore functions are reported separately; they do not require promotion to core functions. Support from representative genes does not establish conservation across the whole family. Missing reviews or identifiers leave a claim unverified.

AnnotonParticipantFunctionAssessment
cg18528_subunit_cg18528 CG18528
UniProtKB:Q9VC87
Gene 		GTPase activity
GO:0003924 		Core Supported
The GO molecular function is reflected in core_functions (exact term or a reviewed subclass).

Gene-review completeness (2/2 grounded genes reviewed)

2 complete review(s) · 2 with deep research · 0 missing review · 0 reviewed but lacking deep research

Gene Review Complete Deep research
CG18528 Q9VC87 ✓ ✓ ✓
CG4610 Q9W245 ✓ ✓ ✓

Details

Context
Drosophila melanogasterNCBITaxon:7227
mitochondrionGO:0005739
GTPBP3-MTO1 complexProtein Complexdmel_gtpbp3_mto1_complex

CG18528 (GTPBP3) and CG4610 (MTO1) complex that modifies mitochondrial tRNA wobble uridines.

GTPBP3-MTO1 complexGO:7770010
Context
Drosophila melanogasterNCBITaxon:7227
mitochondrionGO:0005739
Part 1: GTPase subunit (GTPBP3/MnmE)
CG18528 subunitProtein Complexcg18528_subunit

Annotons

CG18528 CG18528 subunit
cg18528_subunit_cg18528
Participant: Gene Product: CG18528
Gene Product:
CG18528UniProtKB:Q9VC87

Function

GTPase activityGO:0003924

Processes

mitochondrial tRNA wobble uridine modificationGO:0070899
Part 2: FAD-dependent subunit (MTO1/MnmG)
CG4610 subunitProtein Complexcg4610_subunit

Annotons

CG4610 CG4610 subunit
cg4610_subunit_cg4610
Participant: Gene Product: CG4610
Gene Product:
CG4610UniProtKB:Q9W245

Processes

mitochondrial tRNA wobble uridine modificationGO:0070899