Hypusine is a modified lysine found only in translation factor eIF5A, where it is needed for eIF5A to support translation elongation through difficult sequences such as polyproline stretches. It is made in two steps. Deoxyhypusine synthase (Dhps) transfers the aminobutyl group of spermidine to a specific lysine of eIF5A, forming deoxyhypusine. Deoxyhypusine hydroxylase, encoded in Drosophila by nero, then hydroxylates this residue to hypusine. In flies, nero mutations affect cell and organ growth and autophagy.
Boundary. eIF5A is the substrate of both enzymes and is not modelled as a part; spermidine synthesis is upstream context. FlyBase groups realized by this module: HYPUBIO (FBgg0002221). Participants were generated from the FlyBase gene-group membership and mapped to UniProtKB by projects/FLYBASE_GENE_GROUPS/generate_modules.py from module_specs/dmel_hypusine_biosynthesis.yaml. Member genes with a gene review in this repository: Dhps, nero.
All recommended fields populated.
✗ none found
No MODULE:dmel_hypusine_biosynthesis deep-research report alongside the module YAML.
✓ every leaf node grounds to a representative protein.
✓ every declared conforms_to bundle matches its template motif.
✓ every PRECEDES step chains, or its break is acknowledged via chaining_status.
2 gene-function check(s) reflected in core functions · 0 conflict(s) · 0 evidence or coverage gap(s) (2 comparison(s))
Retained noncore functions are reported separately; they do not require promotion to core functions. Support from representative genes does not establish conservation across the whole family. Missing reviews or identifiers leave a claim unverified.
| Annoton | Participant | Function | Assessment |
|---|---|---|---|
| deoxyhypusine_synthesis_dhps | Dhps UniProtKB:Q9VSF4 Gene |
deoxyhypusine synthase activity GO:0034038 |
Core Supported
The GO molecular function is reflected in core_functions (exact term or a reviewed subclass). |
| deoxyhypusine_hydroxylation_nero | nero UniProtKB:Q9V9U4 Gene |
deoxyhypusine monooxygenase activity GO:0019135 |
Core Supported
The GO molecular function is reflected in core_functions (exact term or a reviewed subclass). |
2 complete review(s) · 2 with deep research · 0 missing review · 0 reviewed but lacking deep research
| Gene | Review | Complete | Deep research |
|---|---|---|---|
| Dhps Q9VSF4 | ✓ | ✓ | ✓ |
| nero Q9V9U4 | ✓ | ✓ | ✓ |
Dhps and Nero modify a lysine of eIF5A to hypusine using spermidine.