Integrator complex in Drosophila melanogaster

Integrator is a multisubunit complex that binds promoter-proximal RNA polymerase II and cleaves nascent transcripts. Its endonuclease module, in which IntS11 is the catalytic subunit related to the cleavage and polyadenylation endonuclease Cpsf73, processes the 3' ends of snRNAs and terminates transcription of paused or non-productive polymerases at protein-coding genes, enhancers and long noncoding RNA loci. Integrator also recruits protein phosphatase 2A through IntS6 and IntS8, forming the Integrator-PP2A (INTAC) complex that dephosphorylates the polymerase C-terminal domain and Spt5 and opposes P-TEFb. In Drosophila, Integrator attenuates transcription at many genes and is required for snRNA processing.

MODULE:dmel_integrator_complexDRAFTCONCRETEProtein Complexmodules/dmel_integrator_complex.yaml
integrator complexGO:0032039 INTAC complexGO:0160232
FB:FBgg0002030
FlyBase gene group INT: Integrator complex
FlyBase release FB2026_03 groups 15 D. melanogaster genes as integrator complex (INT); this module models that group.
FB:FBgg0002031
FlyBase gene group INTAC: Integrator-pp2a complex
FlyBase release FB2026_03 groups 17 D. melanogaster genes as integrator-pp2a complex (INTAC); this module models that group.
GO:0032039
integrator complex
GO term grounding the module as integrator complex.
GO:0160232
INTAC complex
GO term grounding the module as INTAC complex.

Boundary. FlyBase groups the core Integrator (INT) and Integrator-PP2A (INTAC); INTAC adds the PP2A catalytic (mts) and scaffolding (Pp2A-29B) subunits, which are general PP2A subunits that also act in many other complexes. The four-part decomposition follows the endonuclease, backbone/shoulder, IntS10-13-14 and phosphatase modules described for human Integrator; IntS3 and IntS12 are placed with the core scaffold for simplicity. FlyBase groups realized by this module: INT (FBgg0002030), INTAC (FBgg0002031). Participants were generated from the FlyBase gene-group membership and mapped to UniProtKB by projects/FLYBASE_GENE_GROUPS/generate_modules.py from module_specs/dmel_integrator_complex.yaml. Member genes with a gene review in this repository: mts, Pp2A-29B.

5Nodes
4Parts
0Variant Sets
0Variants
4Annotons
0Connections

Derived QC

Recommended-field compliance

100.0% recommended fields populated

All recommended fields populated.

Module deep research

✗ none found

No MODULE:dmel_integrator_complex deep-research report alongside the module YAML.

Leaf nodes lacking representative members

✓ every leaf node grounds to a representative protein.

Template conformance

✓ every declared conforms_to bundle matches its template motif.

Function consistency and core coverage

1 gene-function check(s) reflected in core functions · 0 conflict(s) · 1 evidence or coverage gap(s) (2 comparison(s))

Retained noncore functions are reported separately; they do not require promotion to core functions. Support from representative genes does not establish conservation across the whole family. Missing reviews or identifiers leave a claim unverified.

AnnotonParticipantFunctionAssessment
endonuclease_module_complex/ints11 IntS11
UniProtKB:Q9VAH9
Complex Unit 		RNA endonuclease activity
GO:0004521 		Review Missing
No ai-review exists for this protein; its function and core-function coverage cannot yet be checked.
phosphatase_module_complex/mts mts
UniProtKB:P23696
Complex Unit 		protein serine/threonine phosphatase activity
GO:0004722 		Core Supported
The GO molecular function is reflected in core_functions (exact term or a reviewed subclass).

Gene-review completeness (2/17 grounded genes reviewed)

0 complete review(s) · 2 with deep research · 15 missing review · 0 reviewed but lacking deep research

Gene Review Complete Deep research
IntS8 A1ZAK1 ✗ — —
mts P23696 ✓ 88/91 ✓
Pp2A-29B P36179 ✓ 67/69 ✓
IntS3 Q7PLS8 ✗ — —
IntS9 Q95TS5 ✗ — —
IntS11 Q9VAH9 ✗ — —
IntS12 Q9VBB3 ✗ — —
asun Q9VEX5 ✗ — —
omd Q9VFS6 ✗ — —
CG5274 Q9VPF9 ✗ — —
IntS14 Q9VPY0 ✗ — —
defl Q9VT41 ✗ — —
IntS2 Q9VX31 ✗ — —
IntS10 Q9VZM7 ✗ — —
IntS1 Q9W1C5 ✗ — —
IntS4 Q9W3E1 ✗ — —
IntS6 Q9W485 ✗ — —

Details

Context
Drosophila melanogasterNCBITaxon:7227
nucleusGO:0005634
Integrator complexProtein Complexdmel_integrator_complex

Promoter-proximal RNA polymerase II-associated endonuclease and PP2A phosphatase complex for snRNA processing and premature transcription termination.

integrator complexGO:0032039 INTAC complexGO:0160232
Context
Drosophila melanogasterNCBITaxon:7227
nucleusGO:0005634
Part 1: nascent RNA cleavage
Integrator endonuclease moduleProtein Complexendonuclease_module

IntS4, IntS9 and the catalytic endonuclease IntS11.

Annotons

Integrator endonuclease module
endonuclease_module_complex
Participant: Protein Complex: Integrator endonuclease module
Protein Complex:
Integrator endonuclease module
Active units:
IntS4
Participant: Gene Product: IntS4
Gene Product:
IntS4UniProtKB:Q9W3E1
IntS9
Participant: Gene Product: IntS9
Gene Product:
IntS9UniProtKB:Q95TS5
IntS11
Participant: Gene Product: IntS11
Gene Product:
IntS11UniProtKB:Q9VAH9
Function:
RNA endonuclease activityGO:0004521

Processes

snRNA 3'-end processingGO:0034472
Part 2: structural core and polymerase binding
Integrator backbone, shoulder and associated subunitsProtein Complexcore_scaffold

IntS1, IntS2, IntS7 (defl), IntS5 (omd), IntS8, IntS3, IntS12 and IntS15 (CG5274); IntS8 also contributes to PP2A binding.

Annotons

Integrator backbone, shoulder and associated subunits
core_scaffold_complex
Participant: Protein Complex: Integrator backbone, shoulder and associated subunits
Protein Complex:
Integrator backbone, shoulder and associated subunits
Active units:
IntS1
Participant: Gene Product: IntS1
Gene Product:
IntS1UniProtKB:Q9W1C5
IntS2
Participant: Gene Product: IntS2
Gene Product:
IntS2UniProtKB:Q9VX31
defl
Participant: Gene Product: defl
Gene Product:
deflUniProtKB:Q9VT41
omd
Participant: Gene Product: omd
Gene Product:
omdUniProtKB:Q9VFS6
IntS8
Participant: Gene Product: IntS8
Gene Product:
IntS8UniProtKB:A1ZAK1
IntS3
Participant: Gene Product: IntS3
Gene Product:
IntS3UniProtKB:Q7PLS8
IntS12
Participant: Gene Product: IntS12
Gene Product:
IntS12UniProtKB:Q9VBB3
CG5274
Participant: Gene Product: CG5274
Gene Product:
CG5274UniProtKB:Q9VPF9
Part 3: auxiliary module
IntS10-IntS13-IntS14 moduleProtein Complextail_module

IntS10, IntS13 (asun) and IntS14.

Annotons

IntS10-IntS13-IntS14 module
tail_module_complex
Participant: Protein Complex: IntS10-IntS13-IntS14 module
Protein Complex:
IntS10-IntS13-IntS14 module
Active units:
IntS10
Participant: Gene Product: IntS10
Gene Product:
IntS10UniProtKB:Q9VZM7
asun
Participant: Gene Product: asun
Gene Product:
asunUniProtKB:Q9VEX5
IntS14
Participant: Gene Product: IntS14
Gene Product:
IntS14UniProtKB:Q9VPY0
Part 4: RNA polymerase II dephosphorylation in INTAC
Integrator-associated PP2A phosphatase moduleProtein Complexphosphatase_module

IntS6 with the PP2A catalytic subunit mts and the A subunit Pp2A-29B.

Annotons

Integrator-associated PP2A phosphatase module
phosphatase_module_complex
Participant: Protein Complex: Integrator-associated PP2A phosphatase module
Protein Complex:
Integrator-associated PP2A phosphatase module
Active units:
IntS6
Participant: Gene Product: IntS6
Gene Product:
IntS6UniProtKB:Q9W485
mts
Participant: Gene Product: mts
Gene Product:
mtsUniProtKB:P23696
Function:
protein serine/threonine phosphatase activityGO:0004722
Pp2A-29B
Participant: Gene Product: Pp2A-29B
Gene Product:
Pp2A-29BUniProtKB:P36179