Core Mediator
core_mediator_complex
Participant: Protein Complex: Core Mediator
Protein Complex:
Mediator complex in Drosophila melanogaster
Mediator is a large multisubunit coactivator that bridges DNA-bound transcription factors and RNA polymerase II, helping assemble and activate the preinitiation complex. The core Mediator (head, middle and tail modules, with MED14 as a central scaffold) binds the polymerase, while tail subunits contact activators. The CDK8 kinase module (CKM), consisting of Cdk8, CycC, MED12 (kto) and MED13 (skd), associates reversibly with core Mediator and generally restrains its interaction with polymerase; Drosophila kto and skd were identified through their roles in imaginal disc patterning.
mediator complexGO:0016592
FB:FBgg0001867
FlyBase gene group MEDC: Mediator complexes
FlyBase release FB2026_03 groups 30 D. melanogaster genes as mediator complexes (MEDC); this module models that group.
Boundary. The core is modelled as a single complex because head, middle and tail assignments of several metazoan-specific subunits (MED26-MED30) are not uniform across studies. ix is the Drosophila MED29. FlyBase groups realized by this module: MEDC (FBgg0001867). Subgroups folded into it: MED, CKM. Participants were generated from the FlyBase gene-group membership and mapped to UniProtKB by projects/FLYBASE_GENE_GROUPS/generate_modules.py from module_specs/dmel_mediator_complex.yaml. None of the member genes has a gene review in this repository yet.
3Nodes
2Parts
0Variant Sets
0Variants
2Annotons
1Connections
Derived QC
Recommended-field compliance
100.0%
recommended fields populated
All recommended fields populated.
Module deep research
✗ none found
No MODULE:dmel_mediator_complex deep-research report alongside the module YAML.
Leaf nodes lacking representative members
✓ every leaf node grounds to a representative protein.
Template conformance
✓ every declared conforms_to bundle matches its template motif.
Function consistency and core coverage
0 gene-function check(s) reflected in core functions · 0 conflict(s) · 1 evidence or coverage gap(s) (1 comparison(s))
Retained noncore functions are reported separately; they do not require promotion to core functions. Support from representative genes does not establish conservation across the whole family. Missing reviews or identifiers leave a claim unverified.
| Annoton | Participant | Function | Assessment |
|---|---|---|---|
| ckm_complex/cdk8 | Cdk8 UniProtKB:Q9VT57 Complex Unit |
RNA polymerase II CTD heptapeptide repeat kinase activity GO:0008353 |
Review Missing
No ai-review exists for this protein; its function and core-function coverage cannot yet be checked. |
Feedback loops (advisory, prose-driven: a regulator counts as "feedback" when its own description says so)
✓ every negative regulator described as feedback has an upstream edge closing the loop.
-
ckm ⊣ core_mediator [input]
ckm is a negative regulator with no upstream edge (not described as feedback)
Gene-review completeness (0/30 grounded genes reviewed)
0 complete review(s) · 0 with deep research · 30 missing review · 0 reviewed but lacking deep research
| Gene | Review | Complete | Deep research |
|---|---|---|---|
| MED9 A1ZB42 | ✗ | — | — |
| MED8 A1ZBT5 | ✗ | — | — |
| CycC P25008 | ✗ | — | — |
| MED20 P91641 | ✗ | — | — |
| ix Q7KBL8 | ✗ | — | — |
| skd Q7KTX8 | ✗ | — | — |
| MED31 Q8IH24 | ✗ | — | — |
| MED6 Q8MSX2 | ✗ | — | — |
| MED10 Q9GYU7 | ✗ | — | — |
| MED7 Q9GYV9 | ✗ | — | — |
| MED22 Q9V439 | ✗ | — | — |
| MED26 Q9V4F9 | ✗ | — | — |
| MED28 Q9VBQ9 | ✗ | — | — |
| MED25 Q9VDR1 | ✗ | — | — |
| MED17 Q9VEC1 | ✗ | — | — |
| MED27 Q9VNG0 | ✗ | — | — |
| MED1 Q9VP05 | ✗ | — | — |
| MED4 Q9VS38 | ✗ | — | — |
| MED24 Q9VSF2 | ✗ | — | — |
| Cdk8 Q9VT57 | ✗ | — | — |
| MED19 Q9VVL6 | ✗ | — | — |
| MED11 Q9VVS4 | ✗ | — | — |
| kto Q9VW47 | ✗ | — | — |
| MED30 Q9W0P3 | ✗ | — | — |
| MED14 Q9W0P8 | ✗ | — | — |
| MED23 Q9W1X7 | ✗ | — | — |
| MED16 Q9W278 | ✗ | — | — |
| MED21 Q9W5P1 | ✗ | — | — |
| MED18 Q9XZT1 | ✗ | — | — |
| MED15 Q9Y149 | ✗ | — | — |
Details
Context
Drosophila melanogasterNCBITaxon:7227
nucleusGO:0005634
Mediator complexProtein Complexdmel_mediator_complex
Core Mediator coactivator and its reversibly associated CDK8 kinase module.
mediator complexGO:0016592
Context
Drosophila melanogasterNCBITaxon:7227
nucleusGO:0005634
Connections
CKM binding to core Mediator restrains its association with RNA polymerase II.
Part 1: activator-polymerase bridge
Core MediatorProtein Complexcore_mediator
Annotons
Part 2: regulatory kinase module
CDK8 kinase moduleProtein Complexckm
Annotons
CDK8 kinase module
ckm_complex
Participant: Protein Complex: CDK8 kinase module
Protein Complex:
CDK8 kinase moduleGO:1990508
Active units:
Cdk8
Participant: Gene Product: Cdk8
Gene Product:
Cdk8UniProtKB:Q9VT57
Function:
RNA polymerase II CTD heptapeptide repeat kinase activityGO:0008353