The methionine salvage pathway recycles the methylthio group of 5'-methylthioadenosine (MTA), a by-product of polyamine synthesis, back into methionine. MTA phosphorylase (Mtap) cleaves MTA to adenine and 5-methylthioribose-1-phosphate, which is isomerised to methylthioribulose-1-phosphate (Mri1) and dehydrated to a diketo phosphate (CG11134). The bifunctional enolase-phosphatase Enoph converts this to acireductone, which acireductone dioxygenase (Adi1) oxidises to 4-methylthio-2-oxobutanoate. A final transamination regenerates methionine. As in other animals, the pathway in flies lacks the methylthioribose kinase step used by bacteria and plants, because MTA is cleaved phosphorolytically.
Boundary. The final transamination of 4-methylthio-2-oxobutanoate to methionine is carried out by broad-specificity aminotransferases that FlyBase does not include in this group, so it is not modelled. Adi1 is given the iron-dependent dioxygenase activity, which yields the methionine precursor; the nickel-dependent activity that yields methylthiopropionate is a side reaction. Molecular functions follow orthology to the characterized enzymes of other organisms; except for Mtap, the fly enzymes have not been assayed directly. FlyBase groups realized by this module: LMETSA (FBgg0002175). Participants were generated from the FlyBase gene-group membership and mapped to UniProtKB by projects/FLYBASE_GENE_GROUPS/generate_modules.py from module_specs/dmel_methionine_salvage.yaml. Member genes with a gene review in this repository: Adi1, CG11134, Enoph, Mri1, Mtap.
All recommended fields populated.
✗ none found
No MODULE:dmel_methionine_salvage deep-research report alongside the module YAML.
✓ every leaf node grounds to a representative protein.
✓ every declared conforms_to bundle matches its template motif.
✓ every PRECEDES step chains, or its break is acknowledged via chaining_status.
5 gene-function check(s) reflected in core functions · 0 conflict(s) · 0 evidence or coverage gap(s) (5 comparison(s))
Retained noncore functions are reported separately; they do not require promotion to core functions. Support from representative genes does not establish conservation across the whole family. Missing reviews or identifiers leave a claim unverified.
| Annoton | Participant | Function | Assessment |
|---|---|---|---|
| mta_phosphorolysis_mtap | Mtap UniProtKB:Q9V813 Gene |
S-methyl-5-thioadenosine phosphorylase activity GO:0017061 |
Core Supported
The GO molecular function is reflected in core_functions (exact term or a reviewed subclass). |
| mtr1p_isomerization_mri1 | Mri1 UniProtKB:Q9V9X4 Gene |
S-methyl-5-thioribose-1-phosphate isomerase activity GO:0046523 |
Core Supported
The GO molecular function is reflected in core_functions (exact term or a reviewed subclass). |
| mtru1p_dehydration_cg11134 | CG11134 UniProtKB:Q9VY93 Gene |
methylthioribulose 1-phosphate dehydratase activity GO:0046570 |
Core Supported
The GO molecular function is reflected in core_functions (exact term or a reviewed subclass). |
| enolase_phosphatase_enoph | Enoph UniProtKB:Q9VN95 Gene |
acireductone synthase activity GO:0043874 |
Core Supported
The GO molecular function is reflected in core_functions (exact term or a reviewed subclass). |
| acireductone_dioxygenation_adi1 | Adi1 UniProtKB:Q6AWN0 Gene |
acireductone dioxygenase [iron(II)-requiring] activity GO:0010309 |
Core Supported
The GO molecular function is reflected in core_functions (exact term or a reviewed subclass). |
5 complete review(s) · 5 with deep research · 0 missing review · 0 reviewed but lacking deep research
| Gene | Review | Complete | Deep research |
|---|---|---|---|
| Adi1 Q6AWN0 | ✓ | ✓ | ✓ |
| CG11134 Q9VY93 | ✓ | ✓ | ✓ |
| Enoph Q9VN95 | ✓ | ✓ | ✓ |
| Mri1 Q9V9X4 | ✓ | ✓ | ✓ |
| Mtap Q9V813 | ✓ | ✓ | ✓ |
Five-enzyme pathway that converts 5'-methylthioadenosine back to the methionine precursor 4-methylthio-2-oxobutanoate.