Mettl1-Wuho tRNA m7G46 methyltransferase in Drosophila melanogaster

The METTL1-WDR4 complex (Trm8-Trm82 in yeast) methylates guanosine 46 in the variable loop of many tRNAs at the N7 position, forming m7G46, which stabilizes tRNA structure. METTL1 is the S-adenosylmethionine-dependent catalytic subunit and the WD40-repeat protein WDR4 is required for its stability and activity. In Drosophila, the WDR4 ortholog is wuho (wh), which binds Mettl1 and is required for male and female germline development.

MODULE:dmel_mettl1_wuho_methyltransferaseDRAFTCONCRETEProtein Complexmodules/dmel_mettl1_wuho_methyltransferase.yaml
tRNA (m7G46) methyltransferase complexGO:0106143
FB:FBgg0002114
FlyBase gene group METWD: Mettl1-wuho trna (guanine-n(7)-)-methyltransferase complex
FlyBase release FB2026_03 groups 2 D. melanogaster genes as mettl1-wuho trna (guanine-n(7)-)-methyltransferase complex (METWD); this module models that group.
GO:0106143
tRNA (m7G46) methyltransferase complex
GO term grounding the module as tRNA (m7G46) methyltransferase complex.

Modelled one part per subunit. The catalytic activity is assigned to Mettl1 by orthology to METTL1 and Trm8. FlyBase groups realized by this module: METWD (FBgg0002114). Participants were generated from the FlyBase gene-group membership and mapped to UniProtKB by projects/FLYBASE_GENE_GROUPS/generate_modules.py from module_specs/dmel_mettl1_wuho_methyltransferase.yaml. Member genes with a gene review in this repository: Mettl1, wuho.

3Nodes
2Parts
0Variant Sets
0Variants
2Annotons
0Connections

Derived QC

Recommended-field compliance

100.0% recommended fields populated

All recommended fields populated.

Module deep research

✗ none found

No MODULE:dmel_mettl1_wuho_methyltransferase deep-research report alongside the module YAML.

Leaf nodes lacking representative members

✓ every leaf node grounds to a representative protein.

Template conformance

✓ every declared conforms_to bundle matches its template motif.

Function consistency and core coverage

1 gene-function check(s) reflected in core functions · 0 conflict(s) · 0 evidence or coverage gap(s) (1 comparison(s))

Retained noncore functions are reported separately; they do not require promotion to core functions. Support from representative genes does not establish conservation across the whole family. Missing reviews or identifiers leave a claim unverified.

AnnotonParticipantFunctionAssessment
mettl1_subunit_mettl1 Mettl1
UniProtKB:O77263
Gene 		tRNA (guanine(46)-N7)-methyltransferase activity
GO:0008176 		Core Supported
The GO molecular function is reflected in core_functions (exact term or a reviewed subclass).

Gene-review completeness (2/2 grounded genes reviewed)

2 complete review(s) · 2 with deep research · 0 missing review · 0 reviewed but lacking deep research

Gene Review Complete Deep research
Mettl1 O77263 ✓ ✓ ✓
wuho Q9W415 ✓ ✓ ✓

Details

Context
Drosophila melanogasterNCBITaxon:7227
Mettl1-Wuho methyltransferaseProtein Complexdmel_mettl1_wuho_methyltransferase

Mettl1 catalytic and Wuho WD40 subunits that form m7G46 in tRNAs.

tRNA (m7G46) methyltransferase complexGO:0106143
Context
Drosophila melanogasterNCBITaxon:7227
Part 1: catalytic methyltransferase subunit
Mettl1 subunitProtein Complexmettl1_subunit

Annotons

Mettl1 Mettl1 subunit
mettl1_subunit_mettl1
Participant: Gene Product: Mettl1
Gene Product:
Mettl1UniProtKB:O77263

Function

tRNA (guanine(46)-N7)-methyltransferase activityGO:0008176

Processes

tRNA (guanine-N7)-methylationGO:0106004
Part 2: WD40 non-catalytic subunit
wuho subunitProtein Complexwuho_subunit

Annotons

wuho wuho subunit
wuho_subunit_wuho
Participant: Gene Product: wuho
Gene Product:
wuhoUniProtKB:Q9W415

Processes

tRNA (guanine-N7)-methylationGO:0106004