The MRN complex senses DNA double-strand breaks. Rad50, an SMC-like ATPase with a long coiled-coil, tethers DNA ends; Mre11 is a nuclease with 3'-5' exonuclease and single-stranded endonuclease activities that starts resection of break ends; Nbs (Nibrin) mediates recruitment and activation of the ATM kinase. In Drosophila, mre11, rad50 and nbs are also required to prevent telomere fusions.
Catalytic activities are assigned to Mre11 and Rad50 by conservation with the characterized yeast and human proteins. ATM (tefu) and downstream repair factors are outside the module. FlyBase groups realized by this module: MRN (FBgg0001923). Participants were generated from the FlyBase gene-group membership and mapped to UniProtKB by projects/FLYBASE_GENE_GROUPS/generate_modules.py from module_specs/dmel_mrn_complex.yaml. Member genes with a gene review in this repository: mre11, nbs, rad50.
All recommended fields populated.
✗ none found
No MODULE:dmel_mrn_complex deep-research report alongside the module YAML.
✓ every leaf node grounds to a representative protein.
✓ every declared conforms_to bundle matches its template motif.
1 gene-function check(s) reflected in core functions · 0 conflict(s) · 1 evidence or coverage gap(s) (2 comparison(s))
Retained noncore functions are reported separately; they do not require promotion to core functions. Support from representative genes does not establish conservation across the whole family. Missing reviews or identifiers leave a claim unverified.
| Annoton | Participant | Function | Assessment |
|---|---|---|---|
| dmel_mrn_complex_mre11_mre11 | mre11 UniProtKB:Q9XYZ4 Gene |
3'-5'-DNA exonuclease activity GO:0008296 |
Annotation Supported
A retained annotation or curated replacement supports this function, but core_functions does not yet reflect it. |
| dmel_mrn_complex_rad50_rad50 | rad50 UniProtKB:Q9W252 Gene |
ATP hydrolysis activity GO:0016887 |
Core Supported
The GO molecular function is reflected in core_functions (exact term or a reviewed subclass). |
3 complete review(s) · 3 with deep research · 0 missing review · 0 reviewed but lacking deep research
| Gene | Review | Complete | Deep research |
|---|---|---|---|
| mre11 Q9XYZ4 | ✓ | ✓ | ✓ |
| nbs Q9VT40 | ✓ | ✓ | ✓ |
| rad50 Q9W252 | ✓ | ✓ | ✓ |
Double-strand break sensor of Mre11 nuclease, Rad50 ATPase and Nbs that initiates end resection and ATM signalling.
Mre11 nuclease subunit
Nbs subunit for ATM recruitment
Rad50 SMC-like ATPase and DNA-tethering subunit