N-terminal acetyltransferase complexes in Drosophila melanogaster

N-terminal acetyltransferases (NATs) transfer an acetyl group from acetyl-CoA to the alpha-amino group of protein N termini, mostly co-translationally at the ribosome. Each complex pairs a catalytic subunit with one or more auxiliary subunits that anchor it to the ribosome and shape substrate choice. NatA (catalytic Naa10, vnc in flies, with auxiliary Naa15-16) acetylates N termini exposed after removal of the initiator methionine; Naa50 (san) associates with NatA. NatB (Naa20 with Naa25, psidin) acetylates Met-Asp/Glu/Asn/Gln N termini. NatC (Naa30 with Naa35 and Naa38) acetylates Met followed by a hydrophobic residue. Drosophila has a testis-specific NatC catalytic paralog, Naa30B.

MODULE:dmel_n_terminal_acetyltransferase_complexesDRAFTCONCRETEProtein Complexmodules/dmel_n_terminal_acetyltransferase_complexes.yaml
NatA complexGO:0031415 NatB complexGO:0031416 NatC complexGO:0031417
FB:FBgg0000650
FlyBase gene group NAT-CPX: N-terminal acetyltransferase complexes
FlyBase release FB2026_03 groups 9 D. melanogaster genes as n-terminal acetyltransferase complexes (NAT-CPX); this module models that group.
GO:0031415
NatA complex
GO term grounding the module as NatA complex.
GO:0031416
NatB complex
GO term grounding the module as NatB complex.
GO:0031417
NatC complex
GO term grounding the module as NatC complex.

Parts follow FlyBase subgroups NATA, NATB, NATC and NATC-T. Catalytic activity is assigned to the Naa10, Naa20 and Naa30 orthologs by conservation with yeast and human NATs; no activity is assigned to san (Naa50), whose own substrate specificity in flies is not settled here, or to auxiliary subunits. Other NATs that act alone (NatD/Naa40, NatF/Naa60, NatH/Naa80) are not in these FlyBase groups. Naa20B is not a FlyBase member of NATB and is not included. FlyBase groups realized by this module: NAT-CPX (FBgg0000650). Subgroups folded into it: NATA, NATB, NATC, NATC-T. Participants were generated from the FlyBase gene-group membership and mapped to UniProtKB by projects/FLYBASE_GENE_GROUPS/generate_modules.py from module_specs/dmel_n_terminal_acetyltransferase_complexes.yaml. None of the member genes has a gene review in this repository yet.

6Nodes
3Parts
1Variant Sets
2Variants
4Annotons
0Connections

Derived QC

Recommended-field compliance

100.0% recommended fields populated

All recommended fields populated.

Module deep research

✗ none found

No MODULE:dmel_n_terminal_acetyltransferase_complexes deep-research report alongside the module YAML.

Leaf nodes lacking representative members

✓ every leaf node grounds to a representative protein.

Template conformance

✓ every declared conforms_to bundle matches its template motif.

Function consistency and core coverage

0 gene-function check(s) reflected in core functions · 0 conflict(s) · 4 evidence or coverage gap(s) (4 comparison(s))

Retained noncore functions are reported separately; they do not require promotion to core functions. Support from representative genes does not establish conservation across the whole family. Missing reviews or identifiers leave a claim unverified.

AnnotonParticipantFunctionAssessment
nata_complex/vnc vnc
UniProtKB:Q9VT75
Complex Unit 		protein-N-terminal amino-acid acetyltransferase activity
GO:0004596 		Review Missing
No ai-review exists for this protein; its function and core-function coverage cannot yet be checked.
natb_complex/naa20a Naa20A
UniProtKB:M9PI14
Complex Unit 		protein-N-terminal amino-acid acetyltransferase activity
GO:0004596 		Review Missing
No ai-review exists for this protein; its function and core-function coverage cannot yet be checked.
natc_somatic_complex/naa30a Naa30A
UniProtKB:Q95RC0
Complex Unit 		protein-N-terminal amino-acid acetyltransferase activity
GO:0004596 		Review Missing
No ai-review exists for this protein; its function and core-function coverage cannot yet be checked.
natc_testis_complex/naa30b Naa30B
UniProtKB:Q8IRH2
Complex Unit 		protein-N-terminal amino-acid acetyltransferase activity
GO:0004596 		Review Missing
No ai-review exists for this protein; its function and core-function coverage cannot yet be checked.

Gene-review completeness (0/9 grounded genes reviewed)

0 complete review(s) · 0 with deep research · 9 missing review · 0 reviewed but lacking deep research

Gene Review Complete Deep research
Naa20A M9PI14 ✗ — —
Sbat Q8IPZ7 ✗ — —
Naa30B Q8IRH2 ✗ — —
Naa30A Q95RC0 ✗ — —
san Q9NHD5 ✗ — —
psidin Q9VDQ7 ✗ — —
vnc Q9VT75 ✗ — —
Naa15-16 Q9VWI2 ✗ — —
Naa35 Q9W1A2 ✗ — —

Details

Context
Drosophila melanogasterNCBITaxon:7227
N-terminal acetyltransferase complexesProtein Complexdmel_n_terminal_acetyltransferase_complexes

NatA, NatB and NatC ribosome-associated N-terminal acetyltransferase complexes, including a testis-specific NatC variant.

NatA complexGO:0031415 NatB complexGO:0031416 NatC complexGO:0031417
Context
Drosophila melanogasterNCBITaxon:7227
Part 1: N-terminal acetylation after methionine removal
NatA complexProtein Complexnata

Annotons

NatA complex
nata_complex
Participant: Protein Complex: NatA complex
Protein Complex:
NatA complexGO:0031415
Active units:
Naa15-16
Participant: Gene Product: Naa15-16
Gene Product:
Naa15-16UniProtKB:Q9VWI2
Role: auxiliary ribosome-anchoring subunit
san
Participant: Gene Product: san
Gene Product:
sanUniProtKB:Q9NHD5
Role: associated acetyltransferase (Naa50)
vnc
Participant: Gene Product: vnc
Gene Product:
vncUniProtKB:Q9VT75
Role: catalytic subunit (Naa10)
Function:
protein-N-terminal amino-acid acetyltransferase activityGO:0004596

Processes

N-terminal protein amino acid acetylationGO:0006474
Part 2: acetylation of Met-acidic/amide N termini
NatB complexProtein Complexnatb

Annotons

NatB complex
natb_complex
Participant: Protein Complex: NatB complex
Protein Complex:
NatB complexGO:0031416
Active units:
Naa20A
Participant: Gene Product: Naa20A
Gene Product:
Naa20AUniProtKB:M9PI14
Role: catalytic subunit (Naa20)
Function:
protein-N-terminal amino-acid acetyltransferase activityGO:0004596
psidin
Participant: Gene Product: psidin
Gene Product:
psidinUniProtKB:Q9VDQ7
Role: auxiliary subunit (Naa25)

Processes

N-terminal protein amino acid acetylationGO:0006474
Part 3: acetylation of Met-hydrophobic N termini
NatC complexesProtein Complexnatc
Variant set: Somatic or testis-specific NatC (One Or More)
NatC with Naa30AProtein Complexnatc_somatic

Annotons

NatC with Naa30A
natc_somatic_complex
Participant: Protein Complex: NatC with Naa30A
Protein Complex:
NatC with Naa30AGO:0031417
Active units:
Naa30A
Participant: Gene Product: Naa30A
Gene Product:
Naa30AUniProtKB:Q95RC0
Function:
protein-N-terminal amino-acid acetyltransferase activityGO:0004596
Naa35
Participant: Gene Product: Naa35
Gene Product:
Naa35UniProtKB:Q9W1A2
Sbat
Participant: Gene Product: Sbat
Gene Product:
SbatUniProtKB:Q8IPZ7

Processes

N-terminal protein amino acid acetylationGO:0006474
Testis-specific NatC with Naa30BProtein Complexnatc_testis

Annotons

Testis-specific NatC with Naa30B
natc_testis_complex
Participant: Protein Complex: Testis-specific NatC with Naa30B
Protein Complex:
Testis-specific NatC with Naa30BGO:0031417
Active units:
Naa30B
Participant: Gene Product: Naa30B
Gene Product:
Naa30BUniProtKB:Q8IRH2
Function:
protein-N-terminal amino-acid acetyltransferase activityGO:0004596
Naa35
Participant: Gene Product: Naa35
Gene Product:
Naa35UniProtKB:Q9W1A2
Sbat
Participant: Gene Product: Sbat
Gene Product:
SbatUniProtKB:Q8IPZ7

Processes

N-terminal protein amino acid acetylationGO:0006474